JP2021095379A - Method for preparing ginger rhizome extract - Google Patents

Abstract

To provide a method for preparing ginger rhizome extract from ginger rhizome in a manner that is inexpensive and friendly to the environment and human bodies, ginger rhizome extract extracted by the method, and compositions and cosmetics comprising the ginger rhizome extract.SOLUTION: The present disclosure provides a method for preparing ginger rhizome extract by bringing a nanobubble liquid, which contains nanosized fine bubbles, into contact with ginger (Zingiber Officinale) rhizome. There are also provided ginger rhizome extract extracted by the method, and compositions, cosmetics and quasi drugs comprising the ginger rhizome extract.SELECTED DRAWING: Figure 2

Description

The present invention relates to a solution containing nano-sized fine bubbles (also referred to as “nano bubbles” in the present specification and the like) (also referred to as “nano bubble liquid” in the present specification and the like) and ginger (Zingiber Official (Ginger)). The present invention relates to a method for preparing a ginger rhizome extract by contacting with the ginger rhizome, a ginger rhizome extract extracted by the method, and a composition containing the ginger rhizome extract, cosmetics and non-medicinal products.

Ginger is a perennial plant of the family Zingiberaceae and is used as a raw material for condiments and spices. Furthermore, ginger, which is the rhizome of ginger, and its dried product, ginger, have various actions such as stomachic, appetite-promoting, detoxification, anti-inflammatory, antibacterial, cough suppressant, and blood circulation promotion. It is a typical crude drug and is included in Chinese herbal medicines for promoting gastrointestinal function and relieving various cold symptoms. Ginger is also widely used in the fields of cosmetics and pharmaceuticals by external methods.

For example, an extract extracted from ginger rhizome (ginger rhizome extract) is used for the purpose of preventing / ameliorating changes associated with skin aging, that is, skin symptoms such as wrinkles, dullness, loss of texture, and decreased elasticity. It is used (Patent Document 1).

In addition, ginger rhizome extract prevents, reduces or ameliorate dryness, rough skin, cracks, redheads, dandruff, scabies, and inflammatory diseases on the skin, and also prevents and reduces dryness, dryness, split ends, split ends, and hair breakage on the hair. Alternatively, it is blended in cosmetics, bathing agents, detergent compositions and the like that are effective for improving and imparting luster (Patent Document 2).

Furthermore, gingerol and shogaol, which are known as the main active ingredients in ginger rhizome, are known to have blood flow / blood circulation promoting action, antioxidant action, anti-inflammatory action, and melanin production inhibitory action, and are intended for skin beautification effect. (Patent Documents 3, 4 and 5).

JP-A-2009-256272 Japanese Unexamined Patent Publication No. 2001-039823 Japanese Unexamined Patent Publication No. 06-183959 JP-A-2017-002010 Japanese Unexamined Patent Publication No. 2011-178763

Many of the active ingredients in ginger, such as shogaol, are highly lipophilic and extremely poorly soluble in water. As an efficient extraction method, extraction with alcohol is known. For example, refer to Japanese Patent Application Laid-Open No. 2008-565772. However, in the cosmetics market these days, the number of products claiming to be "alcohol-free / ethanol-free" is increasing in order to avoid irritation caused by alcohol, and water-based extraction is more preferable.

From this point of view, a supercritical fluid extraction method (SCFE) is known as an efficient method for extracting ginger. For example, refer to Japanese Patent Application Laid-Open No. 2017-137294. Carbon dioxide in the supercritical fluid state dissolves various substances well, and the supercritical carbon dioxide in which the target substance is dissolved evaporates when it is below the critical point. Therefore, the target substance, that is, the active component of ginger should be efficiently extracted. Can be done. Therefore, a ginger extract (GE) containing a large amount of fat-soluble active ingredient in ginger has been obtained by the supercritical fluid extraction method, and attempts to utilize the extract for various purposes have been studied. There is. However, since the supercritical fluid extraction method uses expensive dedicated equipment, it is necessary to make a large-scale capital investment.

Therefore, the present invention is a method for preparing a ginger rhizome extract from ginger rhizome by an environment-friendly and inexpensive method, a ginger rhizome extract extracted by the method, and a composition containing the ginger rhizome extract. , Cosmetics and non-pharmaceutical products.

As a result of diligent studies to solve the above problems, the present inventors have used a nanobubble solution containing nano-sized fine bubbles to extract ginger rhizome from ginger rhizome (in the present specification, etc., "ginger rhizome". The ginger rhizome nanobubble solution extract obtained by preparing (also referred to as "nano bubble solution extract") has a blood flow promoting function, a moisturizing function, an antioxidant function and a whitening function, which cannot be obtained by the prior art. The heading, the present invention was completed.

Here, the nanobubble liquid is a solution containing fine bubbles having a diameter of nanometer level called nanobubbles. For example, nanobubble water obtained by injecting nanobubbles into water is characterized in that the particles of the nanobubbles are extremely small, so that the bubbles cannot be visually observed like carbonated water for drinking water and are transparent. Nanobubble water is known to have excellent permeability to ordinary water. The effect of nanobubble water varies depending on the type of gas to be nano-sized, but when ozone is used, it has a bactericidal effect on microorganisms and cells (see, for example, International Publication No. 2016/088731). It is known that there is. As a method of using nanobubbles, a bathtub water containing nanobubbles of carbon dioxide is prepared, and the bathtub water is used to dissolve calcium ions and magnesium ions and contain medicinal components of a crude drug, thereby increasing blood flow. A device for manufacturing artificial carbonated hot springs (see, for example, Japanese Patent Application Laid-Open No. 2008-212276), a shower head in which micro-nano bubbles are generated, and a cartridge containing volcanic ash and volcanic lava are combined to provide new functions of detergency and refreshing feeling. There is a shower device to be realized (see, for example, Japanese Patent Application Laid-Open No. 2017-12644).

That is, the present invention includes the following.
(1) A method for preparing a ginger rhizome extract by contacting a nanobubble solution containing nano-sized fine bubbles with the rhizome of ginger (Zingiber Official (Ginger)).
(2) The method according to (1), wherein the nano-sized fine bubbles are carbon dioxide.
(3) The method according to (1) or (2), wherein the nanobubble liquid is nanobubble water.
(4) A ginger rhizome extract extracted from the rhizome of ginger (Zingiber Official (Ginger)) using a nanobubble solution containing nano-sized fine bubbles.
(5) The ginger rhizome extract according to (4), wherein the nano-sized fine bubbles are carbon dioxide.
(6) The ginger rhizome extract according to (4) or (5), wherein the nanobubble liquid is nanobubble water.
(7) A composition containing the ginger rhizome extract according to any one of (4) to (6).
(8) The composition according to (7), which further contains a nanobubble liquid containing nano-sized fine bubbles.
(9) The composition according to (8), wherein the nano-sized fine bubbles are carbon dioxide.
(10) The composition according to (8) or (9), wherein the nanobubble liquid is nanobubble water.
(11) The composition according to any one of (7) to (10), which is a blood flow promoting composition having a blood flow promoting function.
(12) The composition according to any one of (7) to (11), which is a moisturizing composition having a moisturizing function.
(13) A cosmetic containing the ginger rhizome extract according to any one of (4) to (6) or the composition according to any one of (7) to (12).
(14) A quasi-drug containing the ginger rhizome extract according to any one of (4) to (6) or the composition according to any one of (7) to (12).

According to the present invention, a method for preparing a ginger rhizome extract from ginger rhizome by an inexpensive method that is friendly to the environment and the human body, the ginger rhizome extract extracted by the method, a composition containing the ginger rhizome extract, and cosmetics. Fees and non-pharmaceutical products are provided. According to the present invention, active ingredients contained in ginger rhizome such as gingerol and shogaol can be efficiently extracted without requiring a large-scale capital investment, and excellent in blood flow promoting effect, moisturizing property, etc. It is possible to provide a beauty ingredient having a skin-beautifying effect such as an antioxidant effect and an anti-inflammatory effect and having an excellent usability.

It is an image which shows the blood flow after 15 minutes of the test group immersed in each sample. It is a graph which shows the relationship between the addition amount of the sample liquid of Example 1 and Comparative Examples 1 and 2 and the absorbance at 520 nm in the DPPH method.

Hereinafter, the present invention will be described in detail. The present invention is not limited to the following embodiments, and can be carried out in various forms with modifications, improvements, etc. that can be made by those skilled in the art without departing from the gist of the present invention.

The present invention provides a method for preparing a ginger rhizome extract by contacting a ginger rhizome with a nanobubble solution containing nano-sized fine bubbles (nano bubbles), a blood flow promoting function and a moisturizing function extracted by the method. The present invention relates to a ginger rhizome nanobubble solution extract, and a composition, a cosmetic, and a pharmaceutical non-medicinal product containing the ginger rhizome nanobubble solution extract.

The present invention is based on the finding that the ginger rhizome nanobubble liquid extract has an excellent blood flow promoting function and a moisturizing function. The present invention further relates to an extract of ginger rhizome with carbon dioxide nanobubble water (also referred to as "ginger rhizome carbon dioxide nanobubble water extract" in the present specification and the like). The extract of the present invention has an excellent effect of contributing to a blood flow promoting function and a moisturizing function, and can be used as a component of cosmetics, quasi-drugs or pharmaceuticals.

The ginger rhizome used in the present invention is not limited, and various types of ginger rhizome can be used.

A method for preparing a ginger rhizome extract by contacting a nanobubble solution with ginger rhizome is known in the art except that the nanobubble solution prepared as described below is used as the solvent used for the extraction. Any extraction technique can be used.

With respect to the nanobubble liquid to be used, a general treatment that can be generally applied can be arbitrarily selected. For example, a water nanocyclone type multifunctional mixing device (for example, manufactured by CO2 Systems Co., Ltd.) may be used to inject nanobubbles. In this device, by injecting gas at the same time while flowing liquid into the device, a jet water flow can be created by multiple partition plates and slits provided inside the device, and the liquid and gas are compressed inside the device. Mix with. At this time, the gas dissolved in the liquid becomes finer to the nano level and becomes a high-concentration nanobubble liquid.

Examples of the gas to be nano-sized include, but are not limited to, carbon dioxide, nitrogen, oxygen, and ozone, and carbon dioxide and oxygen are preferable, and carbon dioxide is more preferable. The flow rate and temperature of the liquid flowing through the device, and the pressure of the gas, for example, carbon dioxide, are usually 1 L / min when using a small model of a water nanocyclone type multifunctional mixer (manufactured by CO2 Systems Co., Ltd.). ~ 20 L / min, temperature 1 ° C. to 70 ° C., pressure 0.1 Mpa to 1.0 Mpa, flow rate 1 L / min to 10 L / min, temperature 1 ° C. to 60 ° C., pressure 0.1 Mpa to 0.7 Mpa, preferably. Most preferably, the flow rate is 1 L / min to 7 L / min, the temperature is 1 ° C. to 50 ° C., and the pressure is 0.1 Mpa to 0.5 Mpa.

For example, the ginger rhizome nanobubble liquid extract can be obtained by immersing the ginger rhizome in a nanobubble liquid as an extraction solvent, for example, nanobubble water, and eluting the plant components in the extraction solvent. Here, the ginger rhizome may be used as it is for extraction, or may be used for extraction after being processed suitable for the extraction process, for example, drying, cutting to an appropriate size, or crushing. In terms of extraction efficiency, it is preferable that all or part of the ginger rhizome is extracted after drying and crushing. The liquid used for preparing the nanobubble liquid as the extraction solvent includes, but is not limited to, water, a buffer solution, a dilute acid, and a dilute alkali, and any combination or mixture thereof. As the nanobubble liquid, nanobubble water is preferable.

As an extraction method, for example, 5 parts by weight to 100 parts by weight, more preferably 10 parts by weight to 50 parts by weight of crushed ginger rhizome, for example, crushed ginger rhizome is added to 100 parts by weight of the extraction solvent, and the mixture is added to the extraction solvent. Extraction can be performed by immersing the ginger rhizome for a certain period of time, but the method is not limited to this method.

The extraction temperature and extraction time in the extraction method can be appropriately adjusted by those skilled in the art in consideration of the type and amount of the liquid used for preparing the nanobubble liquid. For example, the extraction temperature (temperature of the nanobubble solution) is not limited, but is usually a boiling point of more than 0 ° C. to under pressure, preferably a boiling point of room temperature (10 ° C. to 30 ° C.) to under pressure. More preferably, it can be 15 ° C to 100 ° C, for example 20 ° C to 70 ° C. For example, the extraction time (time to immerse in the nanobubble liquid) varies depending on the type of liquid used for preparing the nanobubble liquid and the extraction temperature, but is usually 1 hour to 6 weeks, preferably 2 hours to 4 weeks, more preferably. It is desirable that it is 3 hours to 1 week. The extraction time can be, for example, 10 hours to 72 hours.

As a preferred example, when nanobubble water is used as the extraction solvent, the extraction temperature is 20 ° C. to 50 ° C., for example, room temperature (10 ° C. to 30 ° C.), and the extraction time is, for example, 1 hour to 2 weeks, preferably 10 hours. It can be up to 72 hours.

After extracting the ginger rhizome with the nanobubble solution as described above, further extraction is performed using water, a buffer solution, a dilute acid, a dilute alkali, their nanobubble solution, and any combination or mixture thereof. You may do it.

The ginger rhizome nanobubble solution extract prepared (extracted) in this manner may be used as it is as the ginger rhizome nanobubble solution extract, but it is said that the extract does not significantly affect the blood flow promoting function and the moisturizing function. The extract may be subjected to conventional treatments that are generally applicable.

For example, the extracted ginger rhizome nanobubble solution extract may be purified by a well-known purification method such as filtration of a precipitate by centrifugation or filtration using a membrane filter. By purifying the ginger rhizome nanobubble liquid extract, the obtained purified product can be suitably used as a skin moisturizer.

Further, a wintering treatment may be carried out on the ginger rhizome nanobubble liquid extract after filtration. It is desirable that the temperature is −30 ° C. to 0 ° C. and the wintering treatment time is 3 to 30 days.

Further, the extracted ginger rhizome nanobubble liquid extract may be subjected to a purification treatment for deodorization and decolorization using an activated charcoal column or the like, and the extract containing the components of the extracted ginger rhizome nanobubble liquid extract is always used. By the method, a concentration treatment, a dilution treatment or a solvent exchange treatment may be carried out.

In addition, the extracted ginger rhizome nanobubble liquid extract, the extract containing the components and the processed product thereof are dried by an arbitrary drying technique such as a heat drying method such as a spray drying method or a freeze drying method to dry the dried product. May be.

In the present invention, the ginger rhizome nanobubble solution extract is a dried ginger rhizome nanobubble solution extract thus prepared, an extract containing the components thereof, and a processed product thereof (for example, a freeze-dried product containing a vacuum freeze-dried product). Includes products, purified products, concentrates, diluents, solutions in solvents other than the extraction solvent, or combinations thereof).

The ginger rhizome nanobubble solution extract according to the present invention may be subjected to conventional sterilization that can be generally applied. The sterilization method is not limited, and examples thereof include heat sterilization and filter sterilization.

The ginger rhizome nanobubble solution extract according to the present invention is further combined with an additional solvent, for example, an additional nanobubble solution to form a composition containing the ginger rhizome nanobubble solution extract and an additional solvent, for example, an additional nanobubble solution. You may. The solid content concentration in the composition containing the ginger rhizome nanobubble liquid extract is not limited, but is usually 0.01% to 10%, preferably 0.1% to 5%, based on the total weight of the composition. is there. For example, the liquid used as the additional solvent may be the same liquid as the extraction solvent in the combined ginger rhizome nanobubble solution extract, but is not limited to water, buffer, dilute acid, dilute alkali, and their nanobubbles. The liquid and any combination or mixture thereof may be used.

The ginger rhizome nanobubble liquid extract according to the present invention exhibits a blood flow promoting function and a moisturizing function as shown in the following examples.

The blood flow promoting function and the moisturizing function of the ginger rhizome nanobubble liquid extract according to the present invention can be measured and evaluated by a conventional method. Specifically, each activity can be measured and evaluated as described in the following examples.

For the blood flow promoting function and the moisturizing function according to the present invention, in vivo skin or skin cells (for example, skin fibroblasts) may be used as an action target, or skin cells (for example, skin fibroblasts) may be used in vitro or ex vivo. (Cells) may be used as the target of action, cells other than skin cells (for example, skin fibroblasts) (for example, fibroblasts in tissues or organs (such as lungs) other than skin) or non-cellular substances such as enzymes and radicals. May be used as an action target.

As described above and below, the ginger rhizome nanobubble liquid extract according to the present invention has a blood flow promoting function and a moisturizing function, and while maintaining high safety, moisturizes the skin and after use. It is less sticky and can be used, for example, in cosmetics (various preparations used for humans and other animals), pharmaceuticals, non-medicinal products, bathing agents, and the like.

Therefore, the ginger rhizome nanobubble liquid extract or composition according to the present invention can be used by blending with the following cosmetics, quasi-drugs or pharmaceuticals. That is, the present invention relates to cosmetics, quasi-drugs or pharmaceuticals containing the ginger rhizome nanobubble liquid extract according to the present invention. Such cosmetics, quasi-drugs or pharmaceuticals according to the present invention may be used orally or parenterally, but when applied as an external preparation, particularly a skin external preparation, each effect can be exhibited. Can be done.

The cosmetics, quasi-drugs or pharmaceuticals according to the present invention may be a composition in any form such as a solution, a suspension, an emulsified product, a powdered product, a paste product, a mousse product or a gel product. The cosmetics, non-pharmaceutical products or pharmaceuticals according to the present invention may be any cosmetics, non-pharmaceutical products or pharmaceuticals that can be used in contact with the skin, and specifically, for example, facial cleansers and emulsions. , Beauty liquid, general cream (face cream, hand cream, body cream, etc.), washing pigment (cleansing cream, etc.), pack, shaving cream, sun cream, sunscreen cream, sunscreen lotion, sunscreen lotion, makeup soap, Foundation, whitening, powder, lipstick, lip cream, eyeliner, eye cream, eyeshadow, mascara, bath cosmetics, shampoo, rinse, hair treatment, hair pack, scalp care agent, body rinse, body gel, antiperspirant deodorant Examples include agents, hair dyes, and hair cosmetics. The amount of the composition containing the ginger rhizome nanobubble solution extract or the ginger rhizome nanobubble solution extract, for example, the ginger rhizome carbon dioxide nanobubble water extract or the ginger rhizome carbon dioxide nanobubble water extract is determined by the cosmetics and pharmaceutical department. The concentration can be 0.001% to 100%, preferably 0.1% to 10%, based on the total weight of the foreign product or the drug. The ginger rhizome nanobubble liquid extract according to the present invention can be blended in any form in cosmetics, quasi-drugs or pharmaceuticals, for example, in liquid form, gel form, powder form, granule form, capsule enclosure and the like. It can be blended in the form.

In addition to the ginger rhizome nanobubble liquid extract, the cosmetics, quasi-drugs or pharmaceuticals according to the present invention may contain any other ingredients that can be incorporated into cosmetics, quasi-drugs or pharmaceuticals. .. Other ingredients include bases, drugs, additives, excipients, and other active ingredients commonly used in cosmetics, quasi-drugs, pharmaceuticals, or bathing agents (eg, other anti-skin aging agents, etc.) Moisturizers, whitening agents, sunscreen agents, astringents, etc.), but are not limited to these, and can be used by arbitrarily selecting and using cosmetics, quasi-drugs, or those suitable for the purpose and form of the drug. Can be done. The cosmetics, quasi-drugs or pharmaceuticals according to the present invention can be produced by a conventional method by mixing and stirring the raw materials.

For example, any of the following components can be added to the cosmetics, quasi-drugs or pharmaceuticals according to the present invention together with the ginger rhizome nanobubble liquid extract.

For example, the following ingredients are exemplified:
Proteins such as collagen, collagen hydrolyzate, gelatin, gelatin hydrolyzate, elastin, elastin hydrolyzate, lactoferrin, keratin, keratin hydrolyzate, casein, albumin, royal jelly-derived protein hydrolyzate, honey-derived protein hydrolyzate And protein hydrolysates;

Natural highs such as sodium hyaluronate and hyaluronic acid derivatives, xanthan gum, carrageenan, guar gum, alginic acid and its salts, pectin, chondroitin sulfate and its salts, water-soluble chitin, chitosan derivatives and their salts, pullulan, deoxyribonucleic acid, arabic rubber, tragant rubber, etc. Molecules and their derivatives;

Fruit-derived products such as licorice extract, aloe extract, chamomile extract, perilla extract, aloe, apple, mandarin orange, thigh, mandarin orange, melon, strawberry, seek arthur, fig, peanut, chestnut, longan, millet, barley, rice, goyomatsu, Seed-derived products such as soybeans, leaf-derived products such as Japanese pear and ginkgo, rhizome-derived products such as wasabi, and plant-derived products such as tree-derived products such as cherry blossoms and peanuts;

Humic acid, fulvic acid in humic acid, which is the final product formed by decomposition of plants by microorganisms;
Crude drug derivatives such as physalis, luo han guo, jujube, adlay, licorice, tochu, and udo root;
Microbial derivatives such as yeast extract;
Bacterial origins such as sclerotium, sclerotium, mushrooms;
Seaweed-derived polysaccharides such as seaweed powder and seaweed extract;
Animal origin such as placenta extract;
Water such as carbonated water, hot spring water, micro-nano bubble water, purified water, distilled water;

Proteins such as collagen, natural polymers such as pullulan, plant-derived products such as fruits and seeds, leaves, stems and roots, tree-derived products, corrosive substances-derived products, crude drug-derived products, fungal-derived products such as mushrooms, placenta, etc. Fermented products obtained by fermenting these, such as animal-derived products;
Carboxyvinyl polymers and salts thereof, polyacrylic acid and salts thereof, acidic polymers such as carboxymethyl cellulose and salts thereof, polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinylpyrrolidone, nitrocellulose, polyvinyl methyl ether and the like. Neutral polymer;

Cationic polymers such as cationized cellulose, polyethyleneimine, cationized guar gum;
Lower alcohols such as ethanol and isopropyl alcohol;
Ultraviolet absorbers such as para-aminobenzoic acid-based UV absorbers, salicylic acid-based UV absorbers, cinnamic acid-based UV absorbers, anthranieric acid-based UV absorbers, and benzophenone-based UV absorbers;

Vitamin A, B vitamins, vitamin C, L-ascorbyl magnesium phosphate and its derivatives, vitamin D group, d-α-tocophenol acetate, vitamin E group, folic acids, β-carotene, γ-orizanol, nicotinic acid, Vitamins such as pantothenic acids, biotins, ferulic acid;

Anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, allantoin;
Antioxidants such as stearic acid ester, nordihydroguaceretenic acid, dibutylhydroxytoluene, butylhydroxyanisole, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol;
Parabenzoic acid esters such as methyl parabenzoate, ethyl parabenzoate, propyl para benzoate, butyl para benzoate, preservatives such as sorbic acid, dehydroacetic acid, phenoxyethanol, benzoic acid;

Edeto acids such as edetic acid and disodium edetate and their salts, sequestrants such as phytic acid and hydroxyethanedisulfonic acid;
Polyhydric alcohols such as glycerin, 1,3-butylene glycol, propylene glycol;
Amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, glycine and salts thereof;
Sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, honey;
Organic acids such as citric acid, lactic acid, α-hydroxyacetic acid, pyrrolidone carboxylic acid and salts thereof;

Bases such as aqueous ammonia, monoethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide;
Hydrocarbons such as liquid paraffin, squalane, petrolatum;
Oils and fats such as olive oil, palm oil, evening primrose oil, jojoba oil, castor oil, and hardened castor oil;
Fatty acids such as lauric acid, myristyl acid, palmitic acid, stearic acid, behenic acid;
Higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol;
Esters such as isopropyl myristate, isopropyl palmitate, cetyl octanoate, glycerin trioctanoate, octyldodecyl myristate, octyl stearate, stearyl stearate;

Phospholipids such as lecithin and its derivatives;
Animal and plant-derived lipids such as bovine bone marrow fat and bovine brain lipids;
Alkyl sulfates such as ammonium lauryl sulfate, ethanolamine lauryl sulfate, sodium lauryl sulfate, and triethanolamine lauryl sulfate;
Polyoxyethylene (2EO) Lauryl Ether Triethanolamine Sulfate (EO is ethylene oxide, and the value before EO indicates the number of moles of ethylene oxide added;), Polyoxyethylene (3EO) alkyl (11 to 15 carbon atoms) One or a mixture of two or more) Polyoxyethylene alkyl sulfates such as ether sodium sulfate;

Alkylbenzene sulfonates such as sodium laurylbenzene sulfonate, triethanolamine laurylbenzene sulfonate;
Polyoxyethylene alkyl ether sulfates such as polyoxyethylene (3EO) tridecyl ether sodium acetate;

Palm oil fatty acid sarcosin sodium, lauroyl sarcosin triethanolamine, lauroyl methyl-L-sodium glutamate, coconut oil fatty acid-L-glutamate sodium, coconut oil fatty acid-L-glutamate triethanolamine, coconut oil fatty acid methyl taurine sodium, lauroyl methyl taurine N-acylamino acid salts such as sodium, sodium ether sulfate alkane sulfonate, hardened coconut oil fatty acid sodium glycerin sulfate, hardened coconut oil fatty acid sodium glycerin sulfate, disodium undecinoylamide ethyl sulfosuccinate, sodium octylphenoxydientoxyethyl sulfonate , Amino sulfosuccinate disodium oleate, dioctyl sulfosuccinate, dioctyl sodium sulfosuccinate, lauryl disodium sulfosuccinate, polyoxyethylene alkyl (carbon chains 12-15) ether phosphate (8EO-10EO), polyoxyethylene oleyl ether Anionic surfactants such as sodium phosphate, sodium polyoxyethylene cetyl ether phosphate, disodium lauryl polyoxyethylene sulfosuccinate, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, sodium tetradecene sulfonate;

Stearyldimethylammonium chloride, di (polyoxyethylene) oleylmethylammonium, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, tri (polyoxyethylene) stearylammonium chloride, polyoxypropylene methyldiethylammonium chloride, myristyldimethylbenzylammonium chloride , Cationic surfactants such as lauryltrimethylammonium chloride;

2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, undecyl hydroxyethyl imidazolinium betaine sodium, undecyl-N-hydroxyethyl-N-carboxymethyl imidazolinium betaine, stearyldihydroxyethyl betaine, palm Oil fatty acid amide propyl betaine, coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine sodium, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethyl imidazolinium disodium lauryl sulfate, N-palm Amphoteric surfactants such as the oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate;

Polyoxyethylene alkyl (12-14 carbon atoms) ether (7EO), polyoxyethylene octylphenyl ether, polyoxyethylene oleyl ether, glycerin polyoxyethylene oleate, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxy Ethylenecetylstearyldiether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyltetradecyl ether, polyoxyethylene lanolin, polyoxy Nonionic surfactants such as ethylene lanolin alcohol and polyoxypropylene stearyl ether;

Isostearic acid diethanolamide, undecylene acid monoethanolamide, oleic acid diethanolamide, beef fatty acid monoethanolamide, hardened beef fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristate diethanolamide, Thickeners such as coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, lanolin fatty acid diethanolamide;

Silicone oils such as chain or cyclic methylpolysiloxane, methylphenylpolysiloxane, dimethylpolysiloxane polyethylene glycol copolymer, dimethylpolysiloxane polypropylene copolymer, amino-modified silicone oil, quaternary ammonium-modified silicone oil;
Thioglycolic acid and its salts;
Cysteamine and its salts;
Peroxides such as hydrogen peroxide, persulfates, borates, urea peroxides;
Bromates such as sodium bromate, potassium bromate;

In addition, pH regulators, colorants, fragrances, stabilizers, refreshing agents, blood flow promoters, keratolytic agents, astringents, wound healing agents, foam thickeners, oral agents, deodorants / deodorants, antiallergic agents Agents, cell activators, inert carriers (solid or liquid carriers), excipients, surfactants, binders, disintegrants, lubricants, solubilizers, suspending agents, coating agents, preservatives, buffers Agent etc.
At least these ingredients can be blended together with the cosmetic of the present invention and used in combination.

The ginger rhizome nanobubble solution extract, composition, or cosmetics, non-pharmaceutical products or pharmaceuticals according to the present invention can be applied to any mammal (subject) including humans, livestock, pet animals, experimental (test) animals, and the like. Can be administered (applied).

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the technical scope of the present invention is not limited to these examples.

1. 1. Sample preparation <Preparation of nanobubble water using carbon dioxide>
Carbon dioxide nanobubbles are injected into a water nanocyclone type multifunctional mixer (manufactured by CO2 Systems Co., Ltd.) with a flow rate of 5 L / min and demineralized water at a water temperature of 38 ° C. 10 L of water was prepared.

Example 1 Ginger rhizomes are crushed into a paste, 20 times by weight of carbon dioxide nanobubble water prepared as described above is added, and the ginger rhizomes are extracted over 3 days at room temperature, passed through a prefilter, and then passed through a prefilter. Filtration was carried out with a 0.45 μm membrane filter to remove insoluble matter. Then, the obtained solution was diluted and adjusted to a dry weight of 1% using carbon dioxide nanobubble water prepared as described above to obtain a ginger rhizome carbon dioxide nanobubble water extract.

Comparative Example 1
An aqueous ginger rhizome ethanol solution extract was obtained in the same manner as in Example 1 except that a 50% (w / w) ethanol aqueous solution was used instead of carbon dioxide nanobubble water.

Comparative Example 2
The ginger rhizome was crushed into a paste, 20 times the amount of hot water at 60 ° C. was added by weight, and the mixture was kept at 60 ° C. or higher for 6 hours of extraction. The obtained suspension was cooled to 30 ° C., passed through a pre-filter, and filtered through a 0.45 μm membrane filter to remove insoluble matter. Then, the obtained solution was diluted and adjusted with water so as to have a dry weight of 1%, and a ginger rhizome hot water extract was obtained.

2. Analysis <Analysis of gingerol and shogaol>
High performance liquid chromatography (high performance liquid chromatography) of the components in the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1, and the ginger rhizome hot water extract prepared in Comparative Example 2. In the present specification and the like, the analysis was performed using “HPLC”) including a high performance liquid chromatogram.

(Environmental conditions of HPLC system)
-Device: Prominence UFCL system column: Sim-pack XR-ODS (75 mm L x 3.0 mm ID, 2.2 μm)
・ Mobile phase: A: Water
B: Acetonylyl
B: Conc. 30% (0.00min) → 90% (2.10min)
→ 90% (2.50 min) → 100% (2.51-3.5 min)
→ 30% (3.51 min)
・ Flow rate: 1.2 ml / min
-Column temperature: 40 ° C
・ Injection amount: 2 μL
-Detection: Photodiode array detector (SPD-M20A) Wavelength 280 nm
・ Cell: Semi-micro cell ・ Detection wavelength: UV280nm

Table 1 shows the peak area values of gingerol and shogaol of Example 1 and Comparative Example 1 with the peak area value of Comparative Example 2 as 100. From the results in Table 1, the carbon dioxide nanobubble water used in the ginger rhizome carbon dioxide nanobubble water extract of Example 1 had the same level of zingeol and the ethanol aqueous solution used in the ginger rhizome ethanol aqueous solution extract of Comparative Example 1. It was confirmed that it has the extraction efficiency of shogaol.

<Blood flow promoting effect>
Ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1, ginger rhizome hot water extract prepared in Comparative Example 2, and blood from a control group (desalted water). The flow promoting effect was evaluated.
(A) Acquisition of capillary images (blood flow images) of fingertips with a microscope (b) Measurement of blood flow with a laser blood flow meter-Number of subjects = 5

In both tests (a) and (b), the starting point was after the entire palm was immersed in a water bath at 10 ° C. for 1 minute, and the blood flow image and blood flow rate at this point were measured.

After the start point, the fingertips are immersed in a sample (Example 1, Comparative Example 1, Comparative Example 2 and demineralized water) kept at 30 ° C., and blood flow images and blood flow rates are obtained after 5 minutes, 10 minutes, and 15 minutes. Was measured.

(A) Capillary image of fingertips by microscope FIG. 1 shows a blood flow image 15 minutes after the test group immersed in each sample. From FIG. 1, when the fingertips were immersed in the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, a clear improvement in the blood flow rate improvement rate was observed from the amount of blood flowing through the capillaries. In addition, the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1 also showed an improvement rate similar to that in Example 1. On the other hand, when the fingertips were immersed in the ginger rhizome hot water extract or desalted water prepared in Comparative Example 2, the blood flowing through the capillaries remained stagnant, and no significant improvement in blood flow was observed.
(B) Blood flow by laser blood flow meter Table 2 shows the results of blood flow by the laser blood flow meter. From Table 2, when the fingertips were immersed in the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, a clear improvement in blood flow improvement rate was observed. In addition, the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1 also showed an improvement rate similar to that in Example 1. On the other hand, when the fingertips were immersed in the ginger rhizome hot water extract or desalinated water prepared in Comparative Example 2, no significant improvement in blood flow was observed.

<Moisturizing effect>
The moisturizing effects of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1, and the ginger rhizome hot water extract prepared in Comparative Example 2 were evaluated.

Four test plots were set in the forearms of 10 subjects, and the water content of each plot was measured. Then, the four test plots were allocated as test plots to which distilled water as Example 1, Comparative Example 1, Comparative Example 2, or Comparative Example 3 was applied, and each sample was applied. Appropriate amounts of the corresponding samples were applied daily to each of these test plots before bedtime. The water content of each test group was measured one week, two weeks, and one month after the start of application of the sample. A moisture meter Corneometer (Corneometer CM825, Curage + Khazaka) was used for measuring the amount of water.

The results are shown in Table 3. From Table 3, while no increase in water content was observed in the distilled water of Comparative Example 3 during the test period, the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1 and the prepared in Comparative Example 1 were prepared. An increase in water content was observed in the test plots to which the ginger rhizome ethanol aqueous solution extract and the ginger rhizome hot water extract prepared in Comparative Example 2 were applied. The effect of increasing the water content was that the test group of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1 had the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1 and the ginger rhizome hot water prepared in Comparative Example 2. It was the highest compared to the extract, and a clear increase in water content, that is, a moisturizing effect was observed. The effect of increasing the water content in the test plot to which the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1 was applied was slightly inferior to that of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, and was prepared in Comparative Example 2. The effect of increasing the water content in the test plot to which the ginger rhizome hot water extract was applied was even lower than that of Comparative Example 1.

<Antioxidant effect>
The antioxidant activity of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1, and the ginger rhizome hot water extract prepared in Comparative Example 2 was evaluated.

As the sample solution, a solution adjusted so that each extract was 1000 ppm in a 50% (w / w) ethanol aqueous solution was used.

Antioxidant activity was measured using the DPPH (1,1-diphenyl-2-picrylhydrazil) method as follows.
0.9 mL of reaction indicator (800 μM 1,1-diphenyl-2-picrylhydrazil 12 mL, 400 mM 2-morpholinoetan sulfonic acid buffer 12 mL, ethanol 24 mL) was dispensed into a test tube, and the sample solution (300-A) was dispensed. μL was added. After about 30 seconds, AμL of the sample solution was added and stirred, and after reacting at room temperature for 20 minutes, the absorbance at 520 nm was measured to measure the radical scavenging activity of the sample solution. Experiments were carried out at A = 0 μL, 30 μL, 60 μL, 120 μL, 180 μL, and 240 μL.

The results are shown in FIG. The decrease in measured absorbance indicates the disappearance of artificially generated DPPH radicals, indicating the radical scavenging activity of the sample solution, that is, the antioxidant capacity. From FIG. 2, it was found that Comparative Example 2 had almost no radical scavenging activity because no decrease in absorbance was observed regardless of the amount of the sample solution added. On the other hand, in the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1 and the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1, the absorbance decreased as the amount of the sample solution added increased. , Concentration-dependent radical scavenging activity was confirmed. Further, the radical scavenging activity of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1 was larger than the radical scavenging activity of the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1. From this result, high antioxidant activity was confirmed in the ginger rhizome carbon dioxide nanobubble water extract in the present invention.

<Tyrosinase inhibitory activity>
The tyrosinase inhibitory activity of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1, the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1, and the ginger rhizome hot water extract prepared in Comparative Example 2 was evaluated.

As the sample solution, a solution adjusted so that each extract was 1000 ppm in a 50% (w / w) ethanol aqueous solution was used.

The tyrosinase inhibitory activity was measured as follows using L-tyrosine as a substrate.
A total of 0.15 mL of 50% (w / w) ethanol solution as a sample solution or control, 0.1 mL of tyrosinase solution (from mushrooms, 1000 U / mL) and phosphate buffer (1 / 15M, pH 6.8) The mixture was added to 2.6 mL and allowed to stand at 25 ° C. for 10 minutes. Then, 0.4 mL of L-tyrosine solution (1.5 mM) was added to this solution and reacted at 25 ° C. for 10 minutes, and then the absorbance of the obtained solution at 470 nm was measured (A: sample solution was added). Absorbance in the case, C: Absorbance when 50% (w / w) ethanol is added). As a blank, a phosphate buffer solution was added instead of the substrate solution L-tyrosine solution, and the absorbance was measured in the same manner (B: absorbance when the sample solution in the blank was added, D: 50% in the blank. (W / w) Absorbance when ethanol is added). The tyrosinase inhibitory activity was calculated by the formula shown below.

Tyrosinase inhibitory activity (%) = (1- (AB) / (CD)) × 100
A: Absorbance when sample solution is added B: Absorbance when sample solution is added in blank C: Absorbance when ethanol is added D: 50% (w / w) ethanol in blank Absorbance when

The results are shown in Table 4. From Table 4, in contrast to the ginger rhizome hot water extract prepared in Comparative Example 2, the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1 and the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1 were used. It was confirmed that there is a clear superiority in the tyrosinase inhibitory activity. Furthermore, the tyrosinase inhibitory activity of the ginger rhizome carbon dioxide nanobubble water extract prepared in Example 1 was larger than that of the ginger rhizome ethanol aqueous solution extract prepared in Comparative Example 1. From this result, high tyrosinase inhibitory activity was confirmed in the ginger rhizome carbon dioxide nanobubble water extract in the present invention.

<Manufacturing of lotion>

According to the formulation shown in Table 5, the components (1) to (10) were stirred at 80 ° C., melted, cooled to room temperature, and mixed with the ginger rhizome carbon dioxide nanobubble water extract of Example 1 and the lotion of Example 2. , Ginger rhizome carbon dioxide nanobubble water extract and the lotion of Comparative Example 4 not mixed with each other were obtained. All of the obtained lotions were clear and stable under the conditions of 40 ° C. and 75% relative humidity (RH) for 3 months without causing cloudiness.

A use test was conducted on the obtained lotions of Example 2 and Comparative Example 4 for 10 specialized panelists. After one month of use, the following items were evaluated on a five-point scale.

(1) Moisturizing skin 1 Drying 2 Slightly dry 3 Normal 4 Slightly moist 5 Moist

(2) Skin smoothness 1 Rough 2 Slightly rough 3 Normal 4 Slightly smooth 5 Smooth

(3) Skin stickiness 1 Sticky 2 Slightly sticky 3 Normal 4 Slightly refreshing 5 Refreshing

Table 6 shows the average score of 10 panelists.

As is clear from Table 6, the lotion of Example 2 containing the ginger rhizome carbon dioxide nanobubble water extract is superior in moisturizing to the lotion not containing the ginger rhizome carbon dioxide nanobubble water extract of Comparative Example 4. It showed sex and skin smoothness.

<Manufacturing of cream agent>

According to the formulation shown in Table 7, (1) to (7) were mixed and stirred at 80 ° C., and (8) to (12) were separately mixed and stirred at 80 ° C., homogenized, and at room temperature while stirring. The mixture was cooled to obtain the cream agent of Example 3.

The obtained cream of Example 3 was not sticky during use and moisturized the skin.

<Manufacturing of body gel agent>

According to the formulation shown in Table 8, (1) to (8) were stirred and dissolved to obtain the body gel agent of Example 4.

The obtained body gel agent of Example 4 was not sticky during use and moisturized the skin.

<Manufacturing of shampoo>

According to the formulation in Table 9, (1) and (2) were heated and dissolved, (3), (4) and (5) were added and stirred at 60 ° C., and (6) to (9) were further added. Stir, then add (10) to pH 5.5-6.0, then dissolve (12) and (13) in (11) and add, and finally add (14). Addition gave the shampoo agent of Example 5.

When the hair was washed with the obtained shampoo agent of Example 5, the feel of the hair became smooth and the hair was moisturized.

Claims (14)

A method for preparing a ginger rhizome extract by contacting a nanobubble solution containing nano-sized fine bubbles with the rhizome of ginger (Zingiber Official). The method according to claim 1, wherein the nano-sized fine bubbles are carbon dioxide. The method according to claim 1 or 2, wherein the nanobubble liquid is nanobubble water. A ginger rhizome extract extracted from the rhizome of ginger (Zingiber Official) using a nanobubble solution containing nano-sized fine bubbles. The ginger rhizome extract according to claim 4, wherein the nano-sized fine bubbles are carbon dioxide. The ginger rhizome extract according to claim 4 or 5, wherein the nanobubble liquid is nanobubble water. A composition containing the ginger rhizome extract according to any one of claims 4 to 6. The composition according to claim 7, further comprising a nanobubble liquid containing nano-sized fine bubbles. The composition according to claim 8, wherein the nano-sized fine bubbles are carbon dioxide. The composition according to claim 8 or 9, wherein the nanobubble liquid is nanobubble water. The composition according to any one of claims 7 to 10, which is a composition for promoting blood flow having a blood flow promoting function. The composition according to any one of claims 7 to 11, which is a moisturizing composition having a moisturizing function. A cosmetic comprising the ginger rhizome extract according to any one of claims 4 to 6 or the composition according to any one of claims 7 to 12. A quasi-drug containing the ginger rhizome extract according to any one of claims 4 to 6 or the composition according to any one of claims 7 to 12.

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