JP2021058174A - Container with lid - Google Patents

Abstract

To provide a technique that can control a fluid volume and fluid level height inside a recess.SOLUTION: A container with a lid has: a tabular member that has a plurality of recesses and has a common opening whose bottom is end faces on the opening sides of the at least two recesses; and a lid member having a contact part that is formed to be able to contact the bottom of the common opening of the tabular member and a plurality of projections that are disposed so as to face the recesses and project from the contact part.SELECTED DRAWING: None

Description

The present invention relates to a container with a lid.

In the safety and efficacy evaluation of compositions such as pharmaceuticals and cosmetics, there is an increasing demand for an evaluation model using cultured human cells as an experimental method that replaces animal experiments. In addition, it is becoming possible to produce various types of cultured human cells using iPS cells.

By the way, in the safety and effectiveness evaluation of a composition using cultured human cells, a container (well) containing cells in a plurality of recesses is used. Further, in order to reduce the cost of the container, a container (multi-well plate) in which the recesses containing cells are reduced and the recesses are integrated is already known.

For example, Patent Document 1 describes a well plate in which the surface of the plate is not contaminated by the sample added to the well and the risk of the sample adhering to the fingers operating the plate is low.

However, in the conventionally used container, it is difficult to control the amount of liquid and the height of the liquid level in the recess without using a dispenser for pouring a constant amount of liquid into the recess.
Therefore, an object of the present invention is to provide a technique capable of controlling the amount of liquid in the recess and the height of the liquid level.

The present invention includes the following aspects.
A plate-shaped member having a plurality of recesses and having a common opening having at least two or more end faces on the opening side of the recess as the bottom surface, and the bottom surface of the common opening of the plate-shaped member can be contacted. A container with a lid, which comprises a contact portion formed on the surface of the container and a lid member provided with a plurality of protrusions arranged so as to face the recess and projecting from the contact portion.

According to the present invention, it is possible to provide a technique capable of controlling the amount of liquid in the recess and the height of the liquid level.

(A) is an exploded perspective view of the container with a lid 10A, and (b) and (c) are partial normal cross-sectional views of the container with a lid 10A. (A) is an exploded perspective view of the container with a lid 10B, and (b) and (c) are partial normal cross-sectional views of the container with a lid 10B. It is an exploded perspective view of the container 10C with a lid. It is an exploded perspective view of the container 10D with a lid. (A) is an exploded perspective view of the container with a lid 10E, (b) is a plan view of the container with a lid 10E, and (c) is a partial normal sectional view of the container with a lid 10E. (A) is an exploded perspective view of the container with a lid 10F, (b) is a plan view of the container with a lid 10F, and (c) is a partial normal sectional view of the container with a lid 10F. (A) is an exploded perspective view of a container with a lid 10G, and (b) and (c) are partial normal cross-sectional views of the container with a lid 10G. (A) is a partial regular cross-sectional view of the plate-shaped member 260, and (b) is a partial regular cross-sectional view of the plate-shaped member 270. (A) and (b) are exploded perspective views of the height positioning portion. (A) is an exploded perspective view of the height positioning portion, and (b) is a partial normal cross-sectional view of the height positioning portion. (A) is a partial regular cross-sectional view of the plate-shaped member 260, and (b) is a partial regular cross-sectional view of the plate-shaped member 270.

A container with a lid according to an embodiment of the present invention will be described with reference to the drawings.

As illustrated in FIGS. 1 and 2, the lidded container 10A is composed of a plate-shaped member 210 and a lid member 310.
In FIGS. 1 to 3, the upper side is referred to as the upper side and the lower side is referred to as the lower side.

As illustrated in FIG. 1 and the like, the plate-shaped member 210 is a plate in which a common opening 21b that opens on one side of the upper surface 21a and a plurality of recesses 21f that are recessed from the bottom surface 21c of the common opening 21b are formed. It is a shaped member. Further, the plate-shaped member 210 is also formed with a drainage passage 21j communicating with the common opening 21b and a liquid sucker insertion hole 21m.

As illustrated in FIG. 1B, the bottom surface 21c of the common opening 21b is a flat surface extending along the top surface 21a of the plate-shaped member 210.
In the plate-shaped member 210, each recess 21f is formed so as to extend perpendicularly to the bottom surface 21c of the common opening 21b. The depth of formation of each recess 21f downward from the bottom surface 21c of the common opening 21b is the same.
The shapes and dimensions of the plurality of recesses 21f of the common opening 21b are the same as each other.

As illustrated in FIGS. 1A and 1B, the lid member 310 has a contact portion 31 and a protrusion 32 protruding from the lower surface 31a of the contact portion 31. The lower surface 31a of the contact portion 31 of the lid member 310 (hereinafter, also referred to as the lower surface of the contact portion) is a flat surface capable of contacting the bottom surface 21c of the common opening 21b of the plate-shaped member 210. By abutting the lower surface 31a of the contact portion 31 with the bottom surface 21c of the common opening 21b of the plate-shaped member 210, it is possible to close all the openings of the recesses 21f that open to the bottom surface 21c of the common opening 21b. Has a size.

The contact portion 31 of the lid member 310 illustrated in FIGS. 1A and 1B has a lower surface 31a having the same external dimensions as the bottom surface 21c of the common opening 21b. The contact portion 31 is a member having a lower surface 31a as an end surface on one side in the axial direction and extending in the axial direction with a cross section equivalent to that of the lower surface 31a.
The contact portion 31 is inserted into the common opening 21b from above while the side surface 31b is inserted along the inner peripheral surface 21e of the common opening 21b, and the lower surface 31a is inserted into the bottom surface 21c of the common opening 21b of the plate-shaped member 210. Can be brought into contact. When the lower surface 31a of the contact portion 31 is brought into contact with the bottom surface 21c of the common opening 21b, the lower surface 31a of the contact portion 31 is in contact with the bottom surface of the common opening 21b around the opening 21g of the recess 21f. The contact portion 31 can close all the openings of the recesses 21f that open to the bottom surface 21c of the common opening 21b by bringing the lower surface 31a into contact with the bottom surface 21c of the common opening 21b of the plate-shaped member 210. ..

The downward protrusion lengths of the contact portions 31 of the protrusions 32 of the lid member 310 from the lower surface 31a are the same as each other. Each protrusion 32 of the lid member 310 projects from the lower surface 31a of the contact portion 31 in a direction perpendicular to the lower surface 31a.
As shown in FIGS. 1A and 1B, the plurality of protrusions 32 of the lid member 310 are formed on the lower surface 31a of the contact portion 31 at positions corresponding to the plurality of recesses 21f of the plate-shaped member 210. ing.
As illustrated in FIG. 1B, the protrusions 32 of the lid member 310 are inserted one by one into the plurality of recesses 21f of the plate-shaped member 210.

As illustrated in FIG. 1B, the formation depth (depth dimension) of the recess 21f of the common opening 21b downward from the bottom surface 21c of the common opening 21b is the protrusion 32 of the lid member 310. It is longer than the downward protrusion length from the lower surface 31a of the contact portion 31 of the lid member 310.
When the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the plate-shaped member 210, the lower surface 31a of the contact portion 31 of the lid member 310 is located above the bottom surface 21i of the recess 21f of the plate-shaped member 210. To do.

As illustrated in FIG. 1B, the cross-sectional dimension perpendicular to the axis of the protrusion 32 (corresponding to the protruding direction perpendicular to the lower surface of the contact portion 31 of the lid member 310) is the recess 21f of the plate-shaped member 210. It matches the cross-sectional dimension perpendicular to the axis (corresponding to the direction perpendicular to the bottom surface 21i of the recess 21f).
Therefore, when the contact portion 31 is brought into contact with the bottom surface 21c of the plate-shaped member 210, the protrusion 32 of the lid member 310 is fitted inside the opening 21g of the recess 21f, and the opening 21g of the recess 21f is liquid-tight. Can be sealed in.

When the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the plate-shaped member 210, each protrusion 32 of the lid member 310 is inserted into the recess 21f of the plate-shaped member 210. In all the recesses 21f into which the protrusions 32 are inserted, the distances from the lower surface of the protrusions 32 in the recesses 21f to the bottom surface 21i of the recesses 21f of the plate-shaped member 210 are the same.

As illustrated in FIGS. 1B and 1C, the drainage passage 21j has a hole shape extending from the common opening 21b along the upper surface 21a of the plate-shaped member 210 inside the plate-shaped member 210. It is formed. The drainage passage 21j extends from the inner peripheral surface 21e of the common opening 21b along the upper surface 21a of the plate-shaped member 210. The drainage bottom surface 21k of the drainage passage 21j is formed flush with the bottom surface 21c of the common opening 21b. Further, the drainage passage 21j illustrated in FIGS. 1, 2 and the like is a non-through hole having no opening other than one end in the extending direction that opens to the inner peripheral surface 21e of the common opening 21b of the plate-shaped member 210. ..

As illustrated in FIGS. 1A and 1B, the liquid sucker insertion hole 21m is formed in the plate-shaped member 210 extending from the upper surface 21a to the drainage passage 21j along the axis perpendicular to the upper surface 21a. It is a hole made. The tip of the liquid absorbing pipe 91 of the liquid absorbing device 90 (pipette or the like) can be inserted into the liquid absorbing device insertion hole 21 m.

Next, an example of a method of controlling the liquid level using the container with a lid 10A will be described.
Here, a case where the cells 2 and the medium 1 are placed in the recess 21f of the plate-shaped member 210 and then the reagent is placed in the recess 21f will be described as an example. As a matter of course, the sample to be put in the recess is not limited to cells and a medium, and may be, for example, a protein.
Cell adhesion is imparted to the bottom surface 21i (hereinafter, also referred to as the bottom surface of the recess) of the recess 21f of the plate-shaped member 210.
First, as shown in FIG. 1A, in a state where the lid member 310 is removed from the plate-shaped member 210, cells 2 (see FIG. 1B) are inserted into the recess 21f of the plate-shaped member 210 to form a bottom surface 21i. Adhere to.
The cell adhesion of the bottom surface 21i is imparted by, for example, plasma treatment, application of a cell adhesion material, or the like. Examples of the cell adhesive material include collagen, fibronectin, laminin, matrigel and the like.
Similarly, in other embodiments, cell adhesion may be imparted to the bottom surface of the recess to allow cells to adhere, but these may be omitted as appropriate.

Next, as shown in FIG. 1A, the medium 1 is injected into the recess 21f. The medium 1 is injected into the recess 21f in an amount capable of filling the recess 21f. Further, the medium 1 is a part or all of the common opening 21b and the drainage passage 21j as shown by the liquid level position shown by the virtual line (dashed line) in FIGS. 1 (a) and 1 (b). You may inject the amount to embed. By injecting an amount of medium 1 that embeds a part or all of the common opening 21b and the drainage passage 21j, the medium 1 can be surely filled in all the recesses 21f.

Subsequently, as illustrated in FIG. 1B, the lid member 310 is brought closer to the opening 21d of the common opening 21b of the plate-shaped member 210, and the protrusion 32 of the lid member 310 is recessed from the common opening 21b. It is inserted into 21f, and the lower surface 31a of the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the common opening 21b. At this time, the medium 1 in the recess 21f of the common opening 21b overflows and flows into the drainage passage 21j.

Subsequently, as illustrated in FIG. 1B, the liquid absorbing pipe 91 of the liquid absorbing device 90 is inserted into the liquid absorbing device insertion hole 21m of the plate-shaped member 210 to reach the drainage passage 21j, and the liquid absorbing device 90 is reached. The medium 1 in the drainage passage 21j is removed by suction.
For suction removal of the medium 1 in the drainage passage 21j by the liquid sucker 90, the tip of the liquid absorption pipe 91 inserted into the liquid sucker insertion hole 21m is inserted into the liquid suction bottom 21k of the drainage passage 21j. This is performed in a state where the height positioning portion 21n, which is a region located on the virtual extension of 21 m, is abutted against the height positioning portion 21n.
The suction removal of the medium 1 in the drainage passage 21j by the liquid sucker 90 is completed by suction-removing all the medium 1 inside the drainage passage 21j and the common opening 21b.

Subsequently, when the lid member 310 is moved upward and the lid member 310 is removed from the plate-shaped member 210, the protrusion 32 is removed from each recess 21f, and the liquid level of the medium 1 stored in each recess 21f The heights are the same as each other.
When the protrusion 32 is inserted into the recess 21f, an amount of medium corresponding to the volume of the protrusion 32 is discharged from the recess 21f. Therefore, when the protrusion 32 is removed from the recess 21f, the liquid level of the medium 1 in the recess 21f is lowered from the bottom surface 21c of the common opening 21b by the amount of liquid corresponding to the volume of the protrusion 32. Will be located.
Therefore, in this liquid level control method, it is possible to secure a space for injecting the analytical reagent into the recess 21f from which the protrusion 32 has been removed. As a result, in this liquid level control method, it is possible to proceed with the analysis of cells 2 for each recess 21f without mixing the mixed solution of the medium 1 and the reagent between the recesses 21f.

The protrusion 32 has a columnar structure extending in the axial direction with a constant cross-sectional dimension whose cross-sectional dimension perpendicular to the axis coincides with the cross-sectional dimension perpendicular to the axis of the recess 21f of the plate-shaped member 210 (FIG. 1A). It is not limited to the configuration exemplified in (c).
The protrusion 32 tightly seals the recess 21f opening in a part or the whole of the recess 21f opening in close contact with the entire circumference of the inner peripheral surface 21h of the recess 21f opening in the axial direction. Any configuration may be used as long as it has a sealing portion.

In the columnar protrusions 32 illustrated in FIGS. 1 (a) to 1 (c), the side peripheral portions forming the entire side surface form the sealing portion.
The recess 21f of the plate-shaped member 210 illustrated in FIGS. 1 (a) to 1 (c) specifically corresponds to a circular hole having a cylindrical inner peripheral surface, and the protrusion 32 matches the inner diameter of the inner peripheral surface of the recess 21f. It has a circular outer diameter and is formed in an extending columnar shape. The inner peripheral surface of the recess 21f is formed so as to extend with a constant inner diameter over the entire length in the axial direction thereof.
However, the cross-sectional shapes of the recess 21f and the protrusion 32 are not limited to a circle and can be changed as appropriate.

The protrusion 32 is not limited to the ones illustrated in FIGS. 1 (a) to 1 (c), and for example, a configuration having a sealing portion only at the end portion (base end portion) on the contact portion 31 side or a base end. It is also possible to adopt a configuration in which a sealing portion is provided only on one or both of the tip portion and the intermediate portion opposite to the portion.
In the case where the protrusion 32 does not have a sealing portion at the tip portion in the axial direction and has a sealing portion extending from the base end in the axial direction to the intermediate portion, the protrusion 32 is inserted into the recess 21f filled with the medium 1 and then inserted into the recess 21f. When removed from the recess 21f, the liquid surface of the medium 1 in the recess 21f is positioned lower than the bottom surface 21c of the common opening 21b by the amount of liquid corresponding to the volume of the protrusion 32.
Further, if the protrusion 32 has a configuration in which the sealing portion exists in the region including the tip in the axial direction, the protrusion 32 is located on a part or the entire side surface on the proximal end side in the axial direction from the sealing portion. Even if there is a recessed portion, the amount of liquid secured in the recess 21f is the same as when the total length in the axial direction is the sealing portion.
The sealing portion of the protrusion 32 may be formed, for example, in a shape having a portion protruding in a ring shape on the tip side as compared with the central portion of the cross section perpendicular to the axis of the protrusion 32.

When the protrusion 32 is inserted into the recess 21f, the entire surface of the recess 21f facing the region of the recess 21f on the bottom surface 21i side is also referred to as a liquid amount adjusting surface. The liquid amount adjusting surface includes a surface on the tip end side of the protrusion 32 of the sealing portion.
The protrusion 32 is a medium 1 filled so as to fill the entire recess 21f when the lower surface 31a of the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the common opening 21b and inserted into the recess 21f. A part of the medium is extruded from the recess 21f, and the volume of the medium 1 is limited to the volume corresponding to the volume on the bottom surface 21i side of the recess 21f from the liquid volume adjusting surface of the protrusion 32 in the recess 21f.

In the container 10B with a lid illustrated in FIGS. 2 (a) to 2 (c), the protrusion 32 of the lid member 310 of the container 1B with a lid of FIG. It is a modified version.
The container with a lid 10B illustrated in FIGS. 2 (a) to 2 (c) has a lid member 320 and a plate-shaped member 210 of a modified example. The lid member 320 of the container 10B with a lid is different from the lid member 320 of the container 10A with a lid of FIG. 1 only in that the protrusion is changed.
In FIGS. 2 (a) to 2 (c), common reference numerals are given to the components common to those in FIGS. 1 (a) to 1 (c), and the description thereof will be omitted or simplified.

As illustrated in FIG. 2B, the cross-sectional dimension perpendicular to the axis of the protrusion 321 (corresponding to the protruding direction perpendicular to the lower surface of the contact portion 31 of the lid member 320) is that of the recess 21f of the common opening 21b. It is smaller than the opening 21g size. The configuration of the protrusion 321 other than the cross-sectional dimensions perpendicular to the axis thereof is the same as that of the protrusion 32 illustrated in FIGS. 1 (a) to 1 (c). The protrusions 32 illustrated in FIGS. 2 (a) to 2 (c) are in close contact with the entire circumferential direction of a part of the inner peripheral surface 21h of the recess 21f opening in the axial direction to make the recess 21f opening liquidtight. It differs from the protrusion 32 illustrated in FIGS. 1 (a) to 1 (c) in that it does not have a sealing portion for sealing.
Therefore, when the contact portion 31 of the lid member 320 is brought into contact with the bottom surface 21c of the plate-shaped member 210 and the protrusion 321 is inserted into the recess 21f, the clearance 21o between the opening 21g of the recess 21f and the protrusion 321 Is secured.

As illustrated in FIGS. 2A and 2B, the horizontal position at which the protrusion 321 of the lid member 320 is inserted into the recess 21f of the common opening 21b is the same as the contact portion 31 of the lid member 320. It can be determined by abutting the inner peripheral portion of the opening 21b. The protrusion 321 of the lid member 320 is inserted, for example, into the center of the opening 21g of the recess 21f of the common opening 21b.

When the contact portion 31 of the lid member 320 is brought into contact with the bottom surface 21c of the plate-shaped member 210, the plurality of protrusions 321 of the lid member 320 have the entire portion protruding from the contact portion 31 in the recess 21f. Will be inserted. The volumes of the portions (portions inserted into the recesses 21f) protruding from the contact portions 31 of the plurality of contact portions 31 of the lid member 310 are aligned with each other.

The lidded container 10B illustrated in FIG. 2 can be used to control the liquid level in the same manner as the lidded container 10A. First, the cells 2 are adhered to the bottom surface 21i of the recess 21f, and then a medium 1 that fills the entire inside of the recess 21f and embeds a part or all of the common opening 21b and the drainage passage 21j is applied to the recess 21f. inject.

Next, the protrusion 321 of the lid member 320 is inserted into the recess 21f from the common opening 21b, and the lower surface 31a of the contact portion 31 of the lid member 320 is brought into contact with the bottom surface 21c of the common opening 21b. By inserting the protrusion 321 into the recess 21f, a part of the medium 1 in the recess 21f is pushed out from the recess 21f into the common opening 21b.

Subsequently, all the medium 1 inside the drainage passage 21j and the common opening 21b is sucked and removed. When the lid member 320 is moved upward and removed from the plate-shaped member 210, the heights of the liquid levels of the medium 1 in each recess 21f of the common opening 21b become uniform.

In the container 10B with a lid of FIG. 2, a clearance 21o is secured between the opening 21g of the recess 21f and the protrusion 321 inserted into the recess 21f. The medium 1 can be smoothly extruded from the recess 21f when the protrusion 321 is inserted.
Further, since the container 10B with a lid of FIG. 2 can secure a large degree of freedom in the shape and dimensions of the protrusion 321 as compared with the container 1B with a lid of FIG. 1, the recess 21f when the protrusion 321 is inserted into the recess 21f It is easy to adjust the amount of the medium 1 extruded from the medium. In the container 10B with a lid of FIG. 2, the amount of the medium 1 in the recess 21f after the protrusion 321 is removed can be finely set depending on the degree of freedom in the shape and size of the protrusion 321.

In the plate-shaped member 220 illustrated in FIG. 3, the liquid sucker insertion hole 21m and the hole-shaped drainage passage 21j are omitted from the plate-shaped member 210 described with reference to FIGS. 1 (a) to 1 (c). A groove-shaped drainage passage 221 recessed from the upper surface 21a is formed. Hereinafter, the drainage passage 221 is also referred to as a drainage groove. Hereinafter, the drainage groove will be described with the same reference numerals as the drainage passage.

As illustrated in FIG. 3, the drainage groove 221 of the plate-shaped member 220 is formed so as to communicate with the opening 21d of the common opening 21b. The drainage groove 221 is formed extending from the inner peripheral surface 21e of the common opening 21b toward the outside of the common opening 21b along the upper surface 21a of the plate-shaped member 220. Further, the drainage groove 221 is a non-penetrating groove in which the tip opposite to the base end on the common opening 21b side reaches the side surface of the plate-shaped member 220 in the extending direction and is not opened.

The groove bottom surface 222 of the drainage groove 221 is a flat surface flush with the bottom surface 21c of the common opening 21b.
The medium 1 that has flowed into the common opening 21b due to the injection of the medium 1 into the recess 21f or the extrusion of the medium 1 from the recess 21f due to the insertion of the protrusion 32 flows into the drainage groove 221 from the common opening 21b. ..

The plate-shaped member 220 of FIG. 3 can suck and remove the medium 1 inside the drainage groove 221 and the common opening 21b by the liquid absorber 90 having the tip of the liquid absorption pipe 91 abutted against the groove bottom surface 222 of the drainage groove 221. ..
The groove bottom surface 222 of the drainage groove 221 of the plate-shaped member 220 of FIG. 3 abuts the tip of the liquid-absorbing pipe 91 of the liquid absorber 90 and positions the tip of the liquid-absorbing pipe 91 with respect to the plate-shaped member 220 in the height direction. It acts as a height positioning unit for the purpose.

The plate-shaped member 230 illustrated in FIG. 4 is a plate-shaped member 220 described with reference to FIG. 3, in which the drainage groove 221 is changed to an external drainage path 231. The external drainage path 231 is different from the drainage groove 221 of the plate-shaped member 220 in that the tip opposite to the base end on the common opening 21b side reaches the side surface of the plate-shaped member 230 and is opened.

The bottom surface 232 of the discharge path of the external discharge path 231 is a flat surface flush with the bottom surface of the common opening 21b, and the medium 1 that has flowed into the common opening 21b from the recess 21f flows into the external discharge path 231 from the common opening 21b. To do.

The plate-shaped member 230 of FIG. 4 can discharge the medium 1 inside the external discharge path 231 and the common opening 21b from the discharge path opening 233 of the external discharge path 231 to the outside of the plate-shaped member 230.
The discharge path bottom surface 232 of the plate-shaped member 230 of FIG. 4 serves as a height positioning portion for abutting the tip of the liquid absorbing pipe 91 of the suction device 90 and positioning the plate-shaped member 230 in the height direction. ..

The suction removal of the surplus medium using the aspirator 90 can be performed more quickly than the case of relying only on the outflow of the surplus medium from the external discharge path 231 and the medium can be reliably returned in the recess 21f direction. Since it can be prevented, it is preferable in terms of preventing contamination.

As illustrated in FIGS. 5 and 6, the container with a lid 10E is composed of a plate-shaped member 240 and a lid member 330.

As illustrated in FIGS. 5 and 6, the plate-shaped member 240 is formed with a common opening 241 that opens to the upper surface 21a, which is one side thereof, and a recess 21f that is recessed from the bottom surface 244 of the common opening 241. .. The lid member 330 has a lid body 331 that comes into contact with the plate-shaped member 240, and a protrusion 332 that is inserted into the recess 21f.

Further, the plate-shaped member 240 is formed with a plurality of groove-shaped common openings 241 and a communication liquid passage 242 that enables liquid movement between the common openings 241. Recesses 21f may be formed at the locations.

As illustrated in FIG. 5A, the plate-shaped member 240 may have a drainage passage 243 in the common opening 241. In the drainage passage 243, the common opening 241 has a drainage passage 243 at both ends in the longitudinal direction. The recess 21f is opened on the bottom surface of the common opening 241. The bottom surface of the common opening 241, the bottom surface of the communication liquid passage 242, and the bottom surface of the drainage passage 243 are flush with each other.

As illustrated in FIG. 5C, when the lid body 331 of the lid member 330 is brought into contact with the upper surface 21a of the plate-shaped member 240, the protrusion 332 is inserted into the recess 21f and becomes the opening 21g of the recess 21f. A clearance 21o is secured between the protrusion 332 and the protrusion 332. The protrusion 332 penetrates the common opening 241 and reaches the recess 21f.

When the lid body 331 of the lid member 330 is brought into contact with the upper surface 21a of the plate-shaped member 240, the plurality of protrusions 332 of the lid member 330 each have a part of a portion protruding from the contact portion 31 in the recess 21f. Will be inserted. The volumes of the portions of the lid member 330 to be inserted into the recesses 21f of the plurality of protrusions 332 are the same as each other.

FIG. 5B is a top view of the plate-shaped member 240 shown in FIG. 5A.

The lidded container 10E illustrated in FIG. 5 can be used to control the liquid level in the same manner as the lidded container 10A. First, the cells 2 are adhered to the bottom surface 21i of the recess 21f, and then the medium 1 in an amount that fills the entire inside of the recess 21f and embeds a part or all of the common opening 241 and the communication liquid passage 242 and the drainage passage 243. Is injected into the recess 21f.

Next, the protrusion 332 of the lid member 330 is inserted into the recess 21f through the common opening 241 so that the lower surface 31a of the contact portion 31 of the lid member 330 is brought into contact with the upper surface 21a of the plate-shaped member 240.
By inserting the protrusion 332 into the recess 21f, a part of the medium 1 in the recess 21f passes through the clearance 21o between the opening 21g of the recess 21f and the protrusion 332, and the common opening 241 and the communication liquid passage 242. Alternatively, it is pushed out to the drainage passage 243.

Next, the tip of the liquid absorbing pipe 91 of the liquid sucker 90 is abutted against the bottom surface 21n of the end of one or more drainage passages 243, and the inside of the common opening 241, the communication liquid passage 242, and the drainage passage 243. Remove all of the medium 1 of the above by suction.
In this container with a lid 10E, even if the protrusion 332 of the lid member 330 is inserted into the recess 21f of the plate-shaped member 240, both portions and the communication liquid passage 242 are separated from each other via the recess 21f in the common opening 241. You can keep the communication state. Therefore, all the medium 1 inside the common opening 241 and the connecting liquid passage 242 can be sucked and removed by the liquid sucker 90 in which the tip of the liquid absorbing pipe 91 is arranged at the end of one drainage passage 243.

Next, when the lid member 330 is moved upward and removed from the plate-shaped member 240, the heights of the liquid levels of the medium 1 in each recess 21f of the plate-shaped member 240 become uniform.

In the container 10E with a lid of FIG. 5, the protrusion 332 of the lid member 330 is inserted into the recess 21f, and the medium 1 overflowing from the recess 21f passes through the common opening 241 and the communication liquid passage 242 and the communication liquid passage 242. The liquid can move between the drain passages 243.

The bottom surfaces of the common opening 241 and the communication liquid passage 242 or the drainage passage 243 are flush with each other. Therefore, at the bottom surface 21n at the end of one or more drainage passages 243, all the medium 1 in the common opening 241 and the communication liquid passage 242 or the drainage passage 243 can be sucked and removed.

The bottom surface 21n at the end of the drainage passage 243 is height-positioned for abutting the tip of the liquid-absorbing pipe 91 of the liquid sucker 90 and positioning the tip of the liquid-absorbing pipe 91 with respect to the plate-shaped member 240 in the height direction. Play the role of a department.

The plate-shaped member 250 illustrated in FIG. 6A differs from the plate-shaped member 240 only in that the concave portion is changed. When the lid body 331 of the lid member 330 is brought into contact with the upper surface 21a of the plate-shaped member 250, a clearance is secured between the protrusion 332 and the inner peripheral surface of the common opening 241.

In the container 10F with a lid of FIG. 6, the heights of the liquid levels in the recess 251 can be made uniform as in the case of using the container 10E with a lid of FIG.

The protrusion 332 tightly seals the recess 251 opening in a part or the whole in the axial direction in close contact with the entire circumference of the inner peripheral surface of the recess 251 opening in the axial direction. It may be configured to have a sealing portion.

In the plate-shaped member 260 illustrated in FIGS. 7 (a) to 7 (c), the common opening 21b of the plate-shaped member 210 of FIG. 1 is changed to the common opening 262 of FIG. The recess 21f is changed to the recess 264 in FIG.

As illustrated in FIGS. 7A to 7C, the lidded container 10G includes a plate-shaped member 260 in which a plurality of common openings 262 and recesses 21f are formed, a lid main body 341, and a protrusion 343. It has a lid member 340.

The plurality of common openings 262 are opened to the upper surface 261 and the recesses 264 are formed by being recessed from the respective bottom surfaces 263 of the common openings 262.

The protrusion 343 of the lid member 340 protrudes from the lower surface 342 of the lid main body 341, is inserted into the common opening 262, and is arranged on the plate-shaped member 260. When the protrusion 343 is inserted into the common opening 262 and brought into contact with the bottom surface 263 of the plate-shaped member 260, the protrusion 343 is brought into close contact with the bottom surface 263 of the common opening 262 and opens to the bottom surface 263 of the common opening 262. Close the opening 265.

The lidded container 10G illustrated in FIGS. 7A to 7C can be used to control the liquid level in the same manner as the lidded container 10A. First, an amount of medium 1 that fills the entire inside of the recess 264 of the plate-shaped member 260 and embeds a part or all of the common opening 262 is injected.

Next, when the protrusion 343 is inserted into the recess 264 and brought into close contact with the common opening 262, the medium 1 overflows to the upper surface 261 of the plate-shaped member 260. When the lid member 340 is removed from the plate-shaped member 260, the liquid level of the medium 1 in the recess 264 is aligned with the bottom surface 263 of the common opening 262.

The plate-shaped members 210 to 260 described so far have a common opening by sucking and removing the medium 1 of the common opening by a liquid sucker 90 in which the tip of the liquid absorbing pipe 91 is abutted against the bottom surface of the common opening from above. It is possible to align the liquid level of the medium 1 in all the recesses that open to the bottom surface of the common opening with the bottom surface of the common opening for each part.
In this specification, the plate-shaped member will be described with its thickness direction (direction perpendicular to the upper surface of the plate-shaped member) as the height direction (hereinafter, also referred to as plate height direction).

The plate-shaped member 260 of FIG. 8A sucks and removes the medium 1 in the common opening 262 by a liquid sucker 90 having the tip of the liquid absorbing pipe 91 abutted against the bottom surface 263 of the common opening 262 from above. Therefore, the liquid level of the medium 1 in the recess 264 can be aligned with the bottom surface 263 of the common opening 262.
The bottom surface 263 of the common opening 262 of the plate-shaped member 260 serves as a height positioning portion for positioning the tip of the liquid absorbing pipe 91 of the liquid sucker 90 abutted from above at the upper end of the recess 264 in the plate height direction. Fulfill.

The plate-shaped member 270 of FIG. 8B has a configuration in which the drainage passage 21j and the liquid sucker insertion hole 21m are omitted from the plate-shaped member 210 of FIGS. 1A to 1C.
In this plate-shaped member 270, the amount of the medium 1 dispensed into the recess 21f that overflows into the common opening 21b is a liquid sucker in which the tip of the liquid absorbing pipe 91 is abutted against the bottom surface 21c of the common opening 21b from above. It can be removed by suction by 90. As a result, in the plate-shaped member 270, the medium 1 in the common opening 21b is sucked and removed by the liquid sucker 90 having the tip of the liquid absorbing pipe 91 abutted against the bottom surface 21c of the common opening 21b from above. The liquid level of the medium 1 in all the recesses 21f that open to the bottom surface 21c of the common opening 21b can be aligned with the bottom surface 21c of the common opening 21b.
The bottom surface 21c of the common opening 21b of the plate-shaped member 270 serves as a height positioning portion for positioning the tip of the liquid absorbing pipe 91 of the liquid sucker 90 abutted from above at the upper end of the recess 21f in the plate height direction. Fulfill.

In the plate-shaped member 270 of FIG. 8B, aligning the liquid level of the medium 1 in each recess 21f with the bottom surface 21c of the common opening 21b is, for example, for each recess 21f opening in the bottom surface 21c of the common opening 21b. It can be used when a reagent having the same composition such as concentration and component is allowed to act on the cells 2 inside. In this case, the medium 1 in the common opening 21b is sucked and removed by the liquid sucker 90 having the tip of the liquid absorbing pipe 91 abutted against the bottom surface 21c of the common opening 21b, and opened to the bottom surface 21c of the common opening 21b. After aligning the liquid levels of the medium 1 in all the recesses 21f with the bottom surface 21c of the common opening 21b, the reagent is injected into each recess 21f, and the medium 1 and the reagent are placed inside each recess 21f and the common opening 21b. Fill the mixture and allow the reagent to act on cells 2.

9 (a) and 9 (b) show an example of the height positioning unit.
9 (a) and 9 (b) show that the height positioning portions 281 and 282 have the height positioning portions 281 and 282, and the opening 21 g of the recess 21 f of the bottom surface 21 c of the common opening 21 b of the plate-shaped member 270 of FIG. An example of applying to the area adjacent to the part) is shown. The height positioning portions 281 and 282 of FIGS. 9A and 9B are formed in a part of the recess 21f in the circumferential direction in the region adjacent to the opening 21g of the recess 21f of the bottom surface 21c of the common opening 21b, respectively. ing.

The height positioning portions 281 and 282 of FIGS. 9A and 9B can also be applied to the bottom surface 21c of the common opening 21b of the plate-shaped member 260 of FIG. 8A.
Further, the height positioning portions 281 and 282 in FIGS. 9A and 9B are common to the upper surfaces of the plate-shaped members of various embodiments such as the plate-shaped members 260 and 270 in FIGS. 11A and 11B. It can also be provided in the area adjacent to the opening.

The height positioning portion 281 of FIG. 9A is a colored portion formed in a region adjacent to the opening 21g of the recess 21f of the bottom surface 21c of the common opening 21b of the plate-shaped member 270 of FIG. 8B. .. As the height positioning portion 281, for example, a part of the bottom surface 21c of the common opening 21b colored in a color different from other regions of the bottom surface 21c by contact with a strong acid or a strong alkali can be adopted.
The height positioning portion 281 serves as a mark indicating the abutting position of the tip of the liquid absorbing pipe 91 of the liquid absorbing device 90 on the bottom surface 21c of the common opening 21b. The height positioning portion 281 is formed so as to be aligned with high accuracy so that the position in the plate height direction coincides with the upper end of the recess 21f. The tip of the liquid absorbing pipe 91 of the liquid absorbing device 90 is positioned with high accuracy so as to coincide with the upper end of the recess 21f in the plate height direction by abutting the tip of the liquid absorbing pipe 91 against the height positioning portion 281 from above.

The height positioning portion 282 of FIG. 9B is formed by recessing from the bottom surface 21c of the common opening 21b in the plate-shaped member 270 of FIG. 8B so as to face the recess 21f (open to the recess 21f). It refers to the vicinity of the notch 282a. The height positioning portion 282 of FIG. 9B includes a notch portion 282a.
The notch 282a is formed in the bottom surface 21c of the common opening 21b in a groove shape extending from the recess 21f with a groove width at which the tip of the liquid absorbing pipe 91 of the liquid sucker 90 cannot be inserted. The cutout portion 282a is formed in a groove shape having a width narrower than that of the tip outer diameter having a circular cross section, for example, the liquid absorbing pipe 91 of the liquid absorbing device 90.

The region near the notch 282a on the bottom surface 21c of the common opening 21b is hereinafter also referred to as a support surface 282b. The height positioning portion 282 of FIG. 9B includes a support surface 282b.
The height positioning portion 282 of FIG. 9B positions the tip of the liquid absorbing pipe 91 of the liquid sucker 90, which is abutted against the support surface 282b from above, at the same position as the upper end of the recess 21f in the plate height direction. .. Further, the liquid absorbent 90 has a common opening by abutting the tip of the liquid absorbing pipe 91 against the support surface 282b so that the liquid absorbing flow path inside the liquid absorbing pipe 91 communicates with the notch 282a of the height positioning portion 282. The medium 1 in the portion 21b can be smoothly sucked and removed through the notch 282a.
Further, the cutout portion 282a of the height positioning portion 282 also serves as a mark indicating the position of the height positioning portion 282.

10 (a) and 10 (b) show the height of the bottom surface of the notch 283 formed by recessing from the bottom surface 21c of the common opening 21b of the plate-shaped member 270 and facing the recess 21f (opening in the recess 21f). The positioning unit 283a is used. The height positioning portion 283a is formed on a flat surface extending perpendicularly to the plate height direction of the plate-shaped member.

The notch 283 is formed in a size capable of inserting the liquid absorbing pipe 91 of the liquid absorbing device 90 from above the bottom surface 21c of the common opening 21b and abutting the tip of the liquid absorbing pipe 91 against the height positioning portion 283a. There is.
The height positioning portion 283a is formed in a size capable of abutting the tip of the liquid absorbing pipe 91 of the liquid absorbing device 90.

The height positioning portion 283a, which is the bottom surface of the notch portions 283 shown in FIGS. 10A and 10B, opens a common opening in the plate height direction of the tip of the liquid absorbing pipe 91 of the liquid absorbing device 90 abutted from above. Positioning is performed at a position shifted downward from the bottom surface 21c of the portion 21b.
In the configurations shown in FIGS. 10A and 10B, the medium 1 in the recess 21f is sucked by the liquid absorber 90 having the tip of the liquid absorbing pipe 91 abutted against the height positioning portion 283a which is the bottom surface of the cutout portion 283. As a result, the position of the liquid level of the medium 1 in the recess 21f in the plate height direction can be aligned with the height positioning portion 283a. The formation position of the height positioning portion 283a, which is the bottom surface of the notch portion 283, in the plate height direction can be appropriately set at a position between the upper end in the depth direction and the lower end in the depth direction of the recess.
Further, the notch portion 283a also serves as a mark indicating the position of the height positioning portion 283a which is the bottom surface thereof.

The notch 283 and the height positioning portion 283a of FIGS. 10A and 10B are also applicable to the bottom surface 21c of the common opening 21b of the plate-shaped member 260 of FIG. 8A.
Further, the notch 283 and the height positioning portion 283a of FIGS. 10 (a) and 10 (b) are upper surfaces of the plate-shaped members of various embodiments such as the plate-shaped members 260 and 270 of FIGS. 11 (a) and 11 (b). It is also possible to form in the area adjacent to the common opening of.

In the configuration in which the height positioning portions of FIGS. 9A and 9B are formed in a region adjacent to the common opening on the upper surface of the plate-shaped member, the height positioning portion is formed on the upper surface of the plate-shaped member due to overflow from the common opening or the like. The existing medium 1 is sucked and removed by a liquid sucker 90 having the tip of the liquid absorbing pipe 91 abutted against the height positioning portion from above, and the position of the medium liquid surface in the common opening in the plate height direction is raised. It can be aligned with the positioning part.
In the configuration in which the height positioning portions of FIGS. 10A and 10B are formed in the region adjacent to the common opening on the upper surface of the plate-shaped member, the medium 1 in the common opening is placed on the height positioning portion from above. The position of the medium liquid surface in the common opening in the plate height direction can be aligned with the height positioning portion by suctioning with the liquid sucker 90 having the tip of the liquid absorbing pipe 91 abutted against it.

The liquid injected into the recess is not limited to the medium, and a buffer solution, a reagent, or the like may be used instead of the medium. For example, carbohydrates such as proteins, lipids, nucleic acids, monosaccharides, and polysaccharides can be stored in the recesses.

The present invention includes the following aspects.
[1] A plate-shaped member having a plurality of recesses and having a common opening having at least two or more end faces on the opening side of the recess as the bottom surface, and the bottom surface of the common opening of the plate-shaped member. A lid characterized by comprising a contact portion formed so as to be abuttable, and a lid member having a plurality of protrusions arranged so as to face the recess and projecting from the contact portion. With container.
[2] The protrusion of the lid member is formed so that the opening of the recess can be liquid-tightly sealed when the contact portion is brought into contact with the bottom surface of the common opening of the plate-shaped member. The container with a lid according to [1].
[3] When the contact portion of the lid member is brought into contact with the bottom surface of the common opening of the plate-shaped member, a clearance is secured between the entire inner peripheral surface of the recess and the protrusion. The container with a lid according to [1].
[4] In the plate-shaped member, a drainage passage through which the liquid discharged from the recess by inserting the protrusion of the lid member into the recess can flow in from the common opening communicates with the common opening. The container with a lid according to any one of [1] to [3], which is formed so as to be formed.
[5] A plate-shaped member formed in a plate shape and having a common opening formed on one surface of the common opening and a recess recessed from the bottom surface of the common opening, and on the upper surface of the plate-shaped member. It has a lid member including a lid body to be mounted on the lid body, and the lid member is projected from the lower surface of the lid body and the upper surface of the plate-shaped member of the lid body. A container with a lid having a protrusion that is inserted into the recess through the common opening when the lower surface is brought into contact with the upper surface of the plate-shaped member.
[6] A plurality of groove-shaped common openings are formed in parallel in the plate-shaped member, and liquid can be transferred between the common openings adjacent to each other between the common openings of the plate-shaped member. The container with a lid according to [5], wherein a communication liquid passage is formed, and the recesses are formed at a plurality of groove-shaped common openings in the extending direction.
[7] A plate-shaped member formed in a plate shape and having a plurality of common openings formed on one side of the common opening and recesses recessed from the bottom surface of each of the common openings, and the plate-shaped member. It has a lid member including a lid body arranged on the upper surface of the lid member, and the lid member faces the lid body and the plate-shaped member when arranged on the plate-shaped member of the lid body. It has a protrusion that protrudes from the lower surface and is inserted into the common opening, and the protrusion is inserted into the common opening of the plate-shaped member and brought into contact with the bottom surface of the common opening. A container with a lid that is in close contact with the entire inner surface of the common opening and closes the opening of the recess that opens to the bottom surface of the common opening.
[8] The container with a lid according to any one of [1] to [7], wherein cell adhesion is imparted to the bottom surface of at least one or more of the recesses.
[9] The container with a lid according to any one of [1] to [8], wherein the cells are adhered to at least one or more of the recesses.
[10] The plate-shaped body is formed with a common opening that opens on the upper surface, which is one side thereof, and one or a plurality of recesses that are recessed from the bottom surface of the common opening, and the recess on the bottom surface of the common opening. The height positioning portion and the height positioning portion for positioning the tip of the liquid absorbing pipe in the depth direction of the recess by contacting the tip of the liquid absorbing pipe of the liquid sucker from above with the region adjacent to the opening of the recess. A visible mark indicating the position of the portion is formed, and the height positioning portion is positioned at the upper end of the recess in the depth direction or between the upper end and the lower end in the depth direction in the depth direction of the recess. A plate-shaped member that extends vertically to form a support surface that supports the tip of the liquid absorbing pipe.
[11] The plate according to [10], wherein the height positioning portion includes a notch formed by recessing from the bottom surface of the common opening so as to face the common opening, and the notch also serves as the mark. Shape member.
[12] The liquid sucker is maintained in a state where the tip of the liquid absorption pipe of the liquid sucker is abutted against the height positioning portion of the plate-shaped member according to [10] or [11] from above. A liquid level height adjusting method in which the liquid in the recess is sucked and the position of the liquid level in the recess in the recess depth direction is aligned with the height positioning portion.

1 ... Medium (sample), 2 ... Cells (sample), 10A ... Lid container, 10B ... Lid container, 10C ... Lid container, 10D ... Lid container, 10E ... Lid container, 10F ... Lid container, 10G ... Container with lid, 21a ... Top surface of plate-shaped member, 21b ... Common opening, 21c ... Bottom surface (of common opening), 21d ... Opening (of common opening), 21e ... Inner circumference (of common opening) Surface, 21f ... concave, 21g ... (recessed) opening, 21h ... (recessed) inner peripheral surface, 21i ... (recessed) bottom surface, 21j ... drainage passage, 21k ... drainage bottom surface, 21m ... liquid absorber Insertion hole, 21n ... height positioning part, 21o ... clearance, 31 ... contact part, 31a ... bottom surface (of contact part), 31b ... side surface (of contact part), 32 ... protrusion, 90 ... liquid absorber , 91 ... Liquid absorbing pipe, 210 ... Plate-shaped member, 220 ... Plate-shaped member, 221 ... Liquid drainage groove, 222 ... Groove bottom surface, 230 ... Plate-shaped member, 231 ... External discharge path, 232 ... Discharge path bottom surface, 233 ... Discharge passage opening, 240 ... Plate-shaped member, 241 ... Common opening, 242 ... Connecting liquid passage, 243 ... Drain passage, 244 ... Bottom surface (of common opening), 250 ... Plate-shaped member, 251 ... Recess, 260 ... plate-shaped member, 261 ... plate-shaped member upper surface, 262 ... common opening, 263 ... bottom surface (of common opening), 264 ... recessed, 265 ... (recessed) opening, 270 ... plate-shaped member, 281 ... high Positioning part (colored part), 282 ... Height positioning part, 282a ... Notch part, 282b ... Support surface, 283 ... Notch part, 283a ... Height positioning part, Support surface (bottom surface of notch part), 310 ... Lid member , 320 ... lid member, 321 ... protrusion, 330 ... lid member, 331 ... lid body, 332 ... protrusion, 340 ... lid member, 341 ... lid body, 342 ... bottom surface (of lid body), 343 ... protrusion

Japanese Unexamined Patent Publication No. 2008-292444

Claims (12)

A plate-shaped member having a plurality of recesses and having a common opening having at least two or more recessed end faces on the opening side as a bottom surface.
A contact portion formed so as to be in contact with the bottom surface of the common opening of the plate-shaped member, and a plurality of protrusions arranged so as to face the recess and projecting from the contact portion. A container with a lid, which comprises a lid member to be provided. A claim in which the protrusion of the lid member is formed so that the opening of the recess can be liquid-tightly sealed when the contact portion is brought into contact with the bottom surface of the common opening of the plate-shaped member. Item 2. The container with a lid according to item 1. Claim that a clearance is secured between the entire inner peripheral surface of the recess and the protrusion when the contact portion of the lid member is brought into contact with the bottom surface of the common opening of the plate-shaped member. The container with a lid according to 1. The plate-shaped member is formed with a drainage passage through which the liquid discharged from the recess by inserting the protrusion of the lid member into the recess communicates with the common opening. The container with a lid according to any one of claims 1 to 3. A plate-shaped member formed in a plate shape and having a common opening formed on one side of the common opening and a recess formed from the bottom surface of the common opening.
It has a lid member including a lid body mounted on the upper surface of the plate-shaped member.
When the lid member is projected from the lower surface of the lid body and the upper surface of the plate-shaped member of the lid body and the lower surface of the lid body is brought into contact with the upper surface of the plate-shaped member. A container with a lid having a protrusion inserted into the recess through the common opening. A plurality of groove-shaped common openings are formed in parallel in the plate-shaped member.
A communication liquid passage is formed between the common openings of the plate-shaped member to allow liquid movement between the common openings adjacent to each other.
The container with a lid according to claim 5, wherein the recesses are formed at a plurality of groove-shaped common openings in the extending direction. A plate-shaped member formed in a plate shape and having a plurality of common openings opened on one surface of the common opening and recesses recessed from the bottom surface of each of the common openings.
It has a lid member having a lid body arranged on the upper surface of the plate-shaped member, and has a lid member.
The lid member has a lid body and a protrusion that protrudes from the lower surface facing the plate-shaped member when placed on the plate-shaped member of the lid body and is inserted into the common opening. ,
The protrusion is inserted into the common opening of the plate-shaped member and brought into contact with the bottom surface of the common opening so as to be brought into close contact with the entire inner surface of the common opening and the recess opened to the bottom surface of the common opening. A container with a lid that closes the opening of the. The container with a lid according to any one of claims 1 to 7, wherein cell adhesion is imparted to the bottom surface of at least one or more of the recesses. The container with a lid according to any one of claims 1 to 8, wherein the cells are adhered to at least one or more of the recesses. The plate-like body is formed with a common opening that opens on the upper surface, which is one side thereof, and one or a plurality of recesses that are recessed from the bottom surface of the common opening.
The tip of the liquid absorbing pipe of the liquid absorber is brought into contact with the region of the bottom surface of the common opening adjacent to the opening of the recess from above, so that the tip of the liquid absorbing pipe is positioned in the depth direction of the recess. A visible mark indicating the position of the height positioning portion and the height positioning portion is formed.
The height positioning portion extends perpendicularly to the depth direction of the recess at a position between the upper end in the depth direction of the recess or between the upper end and the lower end in the depth direction to support the tip of the liquid absorbing pipe. A plate-shaped member that forms a support surface. The plate-shaped member according to claim 10, wherein the height positioning portion includes a notch formed by recessing from the bottom surface of the common opening so as to face the common opening, and the notch also serves as a mark. The state in which the tip of the liquid absorbing pipe of the liquid sucker is abutted against the height positioning portion of the plate-shaped member according to claim 10 or 11 from above is maintained, and the liquid sucker in the recess is provided. A liquid level adjusting method in which a liquid is sucked and the position of the liquid level of the liquid in the recess in the depth direction of the recess is aligned with the height positioning portion.

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