JP7380053B2 - container with lid - Google Patents

Description

The present invention relates to containers with lids.

In evaluating the safety and effectiveness of compositions such as pharmaceuticals and cosmetics, there is an increasing demand for evaluation models using cultured human cells as an alternative experimental method to animal experiments. Furthermore, it is becoming possible to produce various types of cultured human cells using iPS cells.

By the way, in evaluating the safety and effectiveness of compositions using cultured human cells, containers (wells) containing cells in a plurality of recesses are used. Furthermore, in order to reduce the cost of containers, containers (multiwell plates) in which the recesses in which cells are stored are reduced in size and the recesses are integrated are already known.

For example, Patent Document 1 describes a well plate in which the surface of the plate is not contaminated by a sample added to the well, and there is little risk of the sample adhering to fingers operating the plate.

However, in conventional containers, it is difficult to control the amount of liquid and the height of the liquid level in the recess without using a dispenser that pours a fixed amount of liquid into the recess.
Therefore, an object of the present invention is to provide a technique that can control the amount of liquid and the height of the liquid level in the recess.

The present invention includes the following aspects.
a plate-like member having a plurality of recesses and a common opening whose bottom surface is an end face on the opening side of at least two of the recesses; and a plate-like member capable of abutting against the bottom surface of the common opening of the plate-like member. 1. A container with a lid, comprising: a contact portion formed in the recess; and a lid member provided with a plurality of protrusions that are arranged to face the recess and protrude beyond the contact portion.

According to the present invention, it is possible to provide a technique that can control the amount of liquid and the height of the liquid level in the recess.

(a) is an exploded perspective view of the lidded container 10A, and (b) and (c) are partial front sectional views of the lidded container 10A. (a) is an exploded perspective view of the lidded container 10B, and (b) and (c) are partial front sectional views of the lidded container 10B. It is an exploded perspective view of container 10C with a lid. It is an exploded perspective view of container 10D with a lid. (a) is an exploded perspective view of the lidded container 10E, (b) is a plan view of the lidded container 10E, and (c) is a partial front sectional view of the lidded container 10E. (a) is an exploded perspective view of the lidded container 10F, (b) is a plan view of the lidded container 10F, and (c) is a partial front sectional view of the lidded container 10F. (a) is an exploded perspective view of the lidded container 10G, and (b) and (c) are partial front sectional views of the lidded container 10G. (a) is a partial front sectional view of the plate-like member 260, and (b) is a partial front sectional view of the plate-like member 270. (a), (b) is an exploded perspective view of a height positioning part. (a) is an exploded perspective view of the height positioning part, and (b) is a partial front sectional view of the height positioning part. (a) is a partial front sectional view of the plate-like member 260, and (b) is a partial front sectional view of the plate-like member 270.

A container with a lid according to an embodiment of the present invention will be described with reference to the drawings.

As illustrated in FIGS. 1, 2, etc., the lidded container 10A is composed of a plate member 210 and a lid member 310.
Note that in FIGS. 1 to 3, the upper side is referred to as the top and the lower side is referred to as the bottom.

As illustrated in FIG. 1, etc., the plate-like member 210 is a plate in which a common opening 21b that opens on an upper surface 21a, which is one side thereof, and a plurality of recesses 21f that are depressed from a bottom surface 21c of the common opening 21b are formed. It is a shaped member. Further, the plate member 210 is also formed with a liquid drainage passage 21j communicating with the common opening 21b and a liquid absorber insertion hole 21m.

As illustrated in FIG. 1(b), the bottom surface 21c of the common opening 21b is a flat surface extending along the upper surface 21a of the plate-like member 210.
In the plate member 210, each recess 21f is formed to extend perpendicularly to the bottom surface 21c of the common opening 21b. The depth of each recess 21f downward from the bottom surface 21c of the common opening 21b is the same.
The shapes and dimensions of the plurality of recesses 21f of the common opening 21b are the same.

As illustrated in FIGS. 1A and 1B, the lid member 310 includes a contact portion 31 and a protrusion 32 protruding from the lower surface 31a of the contact portion 31. A lower surface 31a (hereinafter also referred to as a lower contact surface) of the contact portion 31 of the lid member 310 is a flat surface that can come into contact with the bottom surface 21c of the common opening 21b of the plate member 210. By bringing the lower surface 31a of the contact portion 31 into contact with the bottom surface 21c of the common opening 21b of the plate-shaped member 210, it is possible to close the openings of all the recesses 21f that open to the bottom surface 21c of the common opening 21b. It has a size.

The contact portion 31 of the lid member 310 illustrated in FIGS. 1(a), 1(b), etc. has a lower surface 31a with the same external dimensions as the bottom surface 21c of the common opening 21b. The contact portion 31 is a member having a lower surface 31a as an end surface on one side in the axial direction and extending in the axial direction with the same cross section as the lower surface 31a.
The contact portion 31 is inserted into the common opening 21b from above while aligning its side surface 31b along the inner circumferential surface 21e of the common opening 21b, and the lower surface 31a is aligned with the bottom surface 21c of the common opening 21b of the plate-shaped member 210. It can be brought into contact. When the lower surface 31a of the contact portion 31 is brought into contact with the bottom surface 21c of the common opening 21b, the lower surface 31a of the contact portion 31 is brought into contact with the bottom surface of the common opening 21b around the opening 21g of the recess 21f. By bringing the lower surface 31a into contact with the bottom surface 21c of the common opening 21b of the plate-shaped member 210, the contact portion 31 can close the openings of all the recesses 21f that open to the bottom surface 21c of the common opening 21b. .

The lengths of the protrusions 32 of the lid member 310 extending downward from the lower surface 31a of the contact portion 31 are the same. Each protrusion 32 of the lid member 310 protrudes from the lower surface 31a of the contact portion 31 in a direction perpendicular to the lower surface 31a.
As shown in FIGS. 1A and 1B, the plurality of protrusions 32 of the lid member 310 are formed on the lower surface 31a of the contact portion 31 at positions corresponding to the plurality of recesses 21f of the plate-like member 210. ing.
As illustrated in FIG. 1(b), the protrusions 32 of the lid member 310 are inserted one by one into the plurality of recesses 21f of the plate-like member 210.

As illustrated in FIG. 1(b), the formation depth (depth dimension) of the recess 21f of the common opening 21b downward from the bottom surface 21c of the common opening 21b is the same as that of the protrusion 32 of the lid member 310. It is longer than the length of the contact portion 31 of the lid member 310 that protrudes downward from the lower surface 31a.
When the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the plate member 210, the lower surface 31a of the contact portion 31 of the lid member 310 is located above the bottom surface 21i of the recess 21f of the plate member 210. do.

As illustrated in FIG. 1(b), the cross-sectional dimension perpendicular to the axis of the protrusion 32 (corresponding to the protrusion direction perpendicular to the lower surface of the contact portion 31 of the lid member 310) is It corresponds to the cross-sectional dimension perpendicular to the axis (which corresponds to the direction perpendicular to the bottom surface 21i of the recess 21f).
Therefore, when the contact part 31 is brought into contact with the bottom surface 21c of the plate member 210, the protrusion 32 of the lid member 310 is fitted inside the opening 21g of the recess 21f, making the opening 21g of the recess 21f liquid-tight. can be sealed.

When the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the plate member 210, each protrusion 32 of the lid member 310 is inserted into the recess 21f of the plate member 210. In all the recesses 21f into which the protrusions 32 are inserted, the distance from the lower surface of the protrusion 32 in the recess 21f to the bottom surface 21i of the recess 21f of the plate-like member 210 is the same.

As illustrated in FIGS. 1(b) and 1(c), the drain passage 21j is a hole-shaped hole extending inside the plate-like member 210 from the common opening 21b along the upper surface 21a of the plate-like member 210. It is formed. The drain passage 21j is formed to extend along the upper surface 21a of the plate member 210 from the inner peripheral surface 21e of the common opening 21b. A drain bottom surface 21k of the drain passage 21j is formed flush with a bottom surface 21c of the common opening 21b. Further, the drain passage 21j illustrated in FIGS. 1, 2, etc. is a non-penetrating hole that does not have an opening other than one end in the extending direction that opens to the inner circumferential surface 21e of the common opening 21b of the plate-shaped member 210. .

As illustrated in FIGS. 1(a) and 1(b), the liquid absorber insertion hole 21m is formed in the plate member 210 so as to extend from the upper surface 21a thereof to the liquid drain passage 21j along the axis perpendicular to the upper surface 21a. It is a hole made by The tip of a liquid suction pipe 91 of a liquid suction device 90 (such as a pipette) can be inserted into the liquid suction device insertion hole 21m.

Next, an example of a method of controlling the liquid level using the lidded container 10A will be described.
Here, an example will be described in which cells 2 and culture medium 1 are placed in the recess 21f of the plate-like member 210, and then a reagent is placed in the recess 21f. Note that, of course, the sample to be placed in the recess is not limited to cells or culture media, and may be, for example, proteins.
The bottom surface 21i of the recess 21f of the plate member 210 (hereinafter also referred to as the bottom surface of the recess) is provided with cell adhesive properties.
First, as shown in FIG. 1(a), with the lid member 310 removed from the plate-like member 210, cells 2 (see FIG. 1(b)) are placed in the recess 21f of the plate-like member 210, and the bottom surface 21i is Glue to.
The cell adhesive property of the bottom surface 21i is imparted by, for example, plasma treatment, application of a cell adhesive material, or the like. Examples of cell adhesive materials include collagen, fibronectin, laminin, matrigel, and the like.
In other embodiments, cell adhesion may be similarly imparted to the bottom surface of the recess to allow cells to adhere thereto, but these may be omitted as appropriate.

Next, as shown in FIG. 1(a), the medium 1 is injected into the recess 21f. The medium 1 is injected into the recess 21f in an amount sufficient to fill the recess 21f. In addition, the culture medium 1 is partially or completely located in the common opening 21b and the drain passage 21j, as shown in the liquid level position indicated by the virtual line (two-dot chain line) in FIGS. 1(a) and 1(b). You can also inject the amount that will embed. By injecting the medium 1 in an amount to fill part or all of the common opening 21b and the drain passage 21j, all the recesses 21f can be reliably filled with the medium 1.

Subsequently, as illustrated in FIG. 1(b), the lid member 310 is brought close to the opening 21d of the common opening 21b of the plate member 210, and the protrusion 32 of the lid member 310 is inserted into the recess from the common opening 21b. 21f, and the lower surface 31a of the contact portion 31 of the lid member 310 is brought into contact with the bottom surface 21c of the common opening 21b. At this time, the culture medium 1 in the recess 21f of the common opening 21b overflows and flows into the drain passage 21j.

Subsequently, as illustrated in FIG. 1(b), the liquid suction pipe 91 of the liquid suction device 90 is inserted into the liquid suction device insertion hole 21m of the plate-shaped member 210 to reach the liquid drainage passage 21j, and the liquid suction device 90 The medium 1 in the drain passage 21j is removed by suction.
The culture medium 1 in the liquid drainage passage 21j is suctioned and removed by the liquid suction device 90 by inserting the tip of the liquid suction pipe 91 inserted into the liquid suction device insertion hole 21m into the liquid suction device insertion hole in the liquid drainage bottom surface 21k of the liquid drainage passage 21j. This is performed in a state in which the height positioning portion 21n, which is an area located on a virtual extension of 21 m, is in contact with the height positioning portion 21n.
The suction removal of the culture medium 1 in the liquid drainage passage 21j by the liquid suction device 90 is completed when all the culture medium 1 inside the liquid drainage passage 21j and the common opening 21b is suctioned and removed.

Subsequently, when the lid member 310 is moved upward and removed from the plate member 210, the protrusion 32 is removed from each recess 21f, and the liquid level of the culture medium 1 stored in each recess 21f is lowered. The heights are the same.
When the protrusion 32 is inserted into the recess 21f, an amount of culture medium corresponding to the volume of the protrusion 32 is discharged from the recess 21f. Therefore, when the protrusion 32 is removed from the recess 21f, the liquid level of the culture medium 1 in the recess 21f is lower than the bottom surface 21c of the common opening 21b by an amount corresponding to the volume of the protrusion 32. It will be located.
Therefore, in this liquid level control method, a space can be secured in the recess 21f from which the protrusion 32 has been removed, allowing the injection of the analytical reagent. As a result, with this liquid level control method, it is possible to proceed with the analysis of the cells 2 for each recess 21f without mixing the mixed liquid of the culture medium 1 and the reagent between the recesses 21f.

Note that the protrusion 32 has a columnar structure extending in the axial direction with a constant dimension in which the cross-sectional dimension perpendicular to the axis coincides with the cross-sectional dimension perpendicular to the axis of the recess 21f of the plate-shaped member 210 (FIG. 1(a) to (c)).
The protrusion 32 liquid-tightly seals the opening of the recess 21f by being in close contact with the entire circumference of the inner peripheral surface 21h of the inner peripheral surface 21h of the opening of the recess 21f in the axial direction. Any structure having a sealing part that does this may be used.

In the columnar protrusion 32 illustrated in FIGS. 1(a) to 1(c), a side peripheral portion that forms the entire side surface constitutes a sealing portion.
Note that the recess 21f of the plate member 210 illustrated in FIGS. 1(a) to (c) is specifically a circular hole having a cylindrical inner peripheral surface, and the protrusion 32 matches the inner diameter of the inner peripheral surface of the recess 21f. It is formed into an extending cylindrical shape with a circular outer diameter cross section. The inner circumferential surface of the recess 21f is formed to extend with a constant inner diameter over its entire axial length.
However, the cross-sectional shapes of the recess 21f and the protrusion 32 are not limited to circular shapes, and can be changed as appropriate.

The protrusion 32 is not limited to those illustrated in FIGS. 1(a) to 1(c), and may have, for example, a structure having a sealing part only at the end (base end) on the side of the abutment part 31, or a structure in which the protrusion It is also possible to adopt a configuration in which the sealing portion is provided only at one or both of the tip portion opposite to the portion and the intermediate portion.
In the case of a configuration in which the protrusion 32 does not have a sealing part at the axially distal end and has a sealing part extending from the axially proximal end to the middle part, after inserting into the recess 21f filled with the culture medium 1, When removed from the recess 21f, the liquid level of the culture medium 1 in the recess 21f is positioned lower than the bottom surface 21c of the common opening 21b by the amount of liquid corresponding to the volume of the protrusion 32.
In addition, if the protrusion 32 has a configuration in which a sealing portion exists in a region including the tip in the axial direction, the protrusion 32 may be formed from the side surface located at the sealing portion on a part or all of the side surface on the proximal end side in the axial direction from the sealing portion. Even if there is a recessed portion, the amount of liquid secured within the recess 21f is the same as when the entire length in the axial direction is the sealing portion.
The sealing portion of the protrusion 32 may be formed, for example, in a shape that has a ring-shaped portion that protrudes toward the tip side compared to the central portion of the cross section of the protrusion 32 perpendicular to its axis.

Regarding the protrusion 32, when inserted into the recess 21f, the entire surface of the recess 21f that faces the area on the bottom surface 21i side of the recess 21f from the protrusion 32 is also referred to as a liquid volume adjustment surface hereinafter. The liquid volume adjustment surface includes a surface on the tip side of the protrusion 32 of the sealing portion.
When the protrusion 32 is inserted into the recess 21f with the lower surface 31a of the abutting portion 31 of the lid member 310 in contact with the bottom surface 21c of the common opening 21b, the protrusion 32 receives the medium 1 filled so as to fill the entire recess 21f. A portion is pushed out from the recess 21f to limit the liquid volume of the culture medium 1 to a volume equivalent to the volume from the liquid volume adjustment surface of the protrusion 32 in the recess 21f to the bottom surface 21i side of the recess 21f.

In the lidded container 10B illustrated in FIGS. 2(a) to 2(c), the protrusion 32 of the lid member 310 of the lidded container 1B in FIG. 1 is replaced with the protrusion 321 shown in FIGS. This has been changed.
The lidded container 10B illustrated in FIGS. 2(a) to 2(c) includes a modified lid member 320 and a plate-like member 210. The lid member 320 of this lidded container 10B differs from the lid member 320 of the lidded container 10A of FIG. 1 only in that the protrusion is changed.
Note that in FIGS. 2(a) to 2(c), components common to those in FIGS. 1(a) to (c) are given the same reference numerals, and their explanations will be omitted or simplified.

As illustrated in FIG. 2(b), the cross-sectional dimension perpendicular to the axis of the protrusion 321 (corresponding to the protrusion direction perpendicular to the lower surface of the contact portion 31 of the lid member 320) is the same as that of the recess 21f of the common opening 21b. It is smaller than the dimension of the opening 21g. Note that the configuration of the protrusion 321 other than the cross-sectional dimension perpendicular to its axis is the same as that of the protrusion 32 illustrated in FIGS. 1(a) to (c). The protrusion 32 illustrated in FIGS. 2(a) to 2(c) is in close contact with the entire circumference of a part of the inner peripheral surface 21h of the opening of the recess 21f in the axial direction to make the opening of the recess 21f liquid-tight. This differs from the protrusion 32 illustrated in FIGS. 1(a) to (c) in that it does not have a sealing portion.
Therefore, when the contact portion 31 of the lid member 320 is brought into contact with the bottom surface 21c of the plate member 210 and the protrusion 321 is inserted into the recess 21f, a clearance 21o is provided between the opening 21g of the recess 21f and the protrusion 321. is ensured.

As illustrated in FIGS. 2(a) and 2(b), the horizontal position where the protrusion 321 of the lid member 320 is inserted into the recess 21f of the common opening 21b is the same as the contact portion 31 of the lid member 320. This can be determined by the abutment of the inner circumference of the opening 21b. The protrusion 321 of the lid member 320 is inserted, for example, into the center of the opening 21g of the recess 21f of the common opening 21b.

When the contact portion 31 of the lid member 320 is brought into contact with the bottom surface 21c of the plate member 210, the entire portion of each of the plurality of protrusions 321 of the lid member 320 that protrudes from the contact portion 31 is in the recess 21f. inserted. The volumes of the portions of the plurality of abutting portions 31 of the lid member 310 that protrude from the abutting portions 31 (the portions that are inserted into the recessed portions 21f) are made equal to each other.

The lidded container 10B illustrated in FIG. 2 can be used to control the liquid level similarly to the lidded container 10A. First, the cells 2 are adhered to the bottom surface 21i of the recess 21f, and then an amount of the medium 1 that fills the entire interior of the recess 21f and embeds a part or all of the common opening 21b and the drain passage 21j is poured into the recess 21f. inject.

Next, the protrusion 321 of the lid member 320 is inserted into the recess 21f from the common opening 21b, and the lower surface 31a of the contact portion 31 of the lid member 320 is brought into contact with the bottom surface 21c of the common opening 21b. By inserting the protrusion 321 into the recess 21f, a portion of the culture medium 1 within the recess 21f is pushed out from the recess 21f to the common opening 21b.

Subsequently, all of the culture medium 1 inside the drain passage 21j and the common opening 21b is removed by suction. When the lid member 320 is moved upward and removed from the plate member 210, the liquid level of the culture medium 1 in each recess 21f of the common opening 21b becomes equal in height.

In the lidded container 10B of FIG. 2, a clearance 21o is ensured between the opening 21g of the recess 21f and the protrusion 321 inserted into the recess 21f, so compared to the lidded container 1B of FIG. When the protrusion 321 is inserted, the culture medium 1 can be smoothly extruded from the recess 21f.
In addition, since the container 10B with a lid shown in FIG. 2 can secure a greater degree of freedom in the shape and dimensions of the protrusion 321 than the container 1B with a lid shown in FIG. It is easy to adjust the amount of medium 1 to be extruded from the container. In the lidded container 10B of FIG. 2, the amount of liquid of the culture medium 1 in the recess 21f after the protrusion 321 is removed can be finely set by the degree of freedom in the shape and size of the protrusion 321.

The plate-like member 220 illustrated in FIG. 3 is the same as the plate-like member 210 described with reference to FIGS. A groove-shaped drainage passage 221 is formed that is depressed from the upper surface 21a. Hereinafter, this drain passage 221 will also be referred to as a drain groove. Note that, in the following explanation, the drain groove is given the same reference numeral as the drain passage.

As illustrated in FIG. 3, the drain groove 221 of the plate member 220 is formed to communicate with the opening 21d of the common opening 21b. The drain groove 221 is formed to extend along the upper surface 21a of the plate member 220 from the inner peripheral surface 21e of the common opening 21b toward the outside of the common opening 21b. Further, in the extending direction, the distal end of the drain groove 221 opposite to the base end on the side of the common opening 21b reaches the side surface of the plate-shaped member 220, and is a non-penetrating groove that is not opened.

A groove bottom surface 222 of the drain groove 221 is a flat surface flush with the bottom surface 21c of the common opening 21b.
The medium 1 that flows into the common opening 21b when the medium 1 is injected into the recess 21f or when the medium 1 is pushed out of the recess 21f when the protrusion 32 is inserted flows into the drain groove 221 from the common opening 21b. .

In the plate member 220 of FIG. 3, the culture medium 1 inside the drain groove 221 and the common opening 21b can be removed by suction using a liquid sucker 90 in which the tip of the liquid suction pipe 91 is brought into contact with the groove bottom surface 222 of the drain groove 221. .
The groove bottom surface 222 of the drain groove 221 of the plate-like member 220 in FIG. It plays the role of a height positioning part.

The plate-like member 230 illustrated in FIG. 4 is the same as the plate-like member 220 described with reference to FIG. The external drain path 231 differs from the drain groove 221 of the plate-shaped member 220 in that the tip opposite to the base end on the side of the common opening 21b reaches the side surface of the plate-shaped member 230 and opens.

The bottom surface 232 of the external discharge path 231 is a flat surface that is flush with the bottom surface of the common opening 21b, and the culture medium 1 that has flowed into the common opening 21b from the recess 21f flows into the external discharge path 231 from the common opening 21b. do.

The plate member 230 in FIG. 4 can discharge the culture medium 1 inside the external discharge path 231 and the common opening 21b to the outside of the plate member 230 from the discharge path opening 233 of the external discharge path 231.
The bottom surface 232 of the discharge path of the plate member 230 in FIG. 4 serves as a height positioning portion for positioning the tip of the liquid suction pipe 91 of the suction device 90 in the height direction with respect to the plate member 230. .

Suctioning and removing the surplus medium using the suction device 90 allows for faster removal compared to relying only on the outflow of the surplus medium from the external discharge path 231, and also ensures that the medium returns toward the recess 21f. This is preferable in terms of preventing contamination.

As illustrated in FIGS. 5 and 6, the lidded container 10E includes a plate member 240 and a lid member 330.

As illustrated in FIGS. 5 and 6, the plate member 240 has a common opening 241 that opens on the upper surface 21a, which is one surface thereof, and a recess 21f that is depressed from the bottom surface 244 of the common opening 241. . The lid member 330 has a lid main body 331 that comes into contact with the plate member 240, and a protrusion 332 that is inserted into the recess 21f.

Further, the plate-like member 240 is formed with a plurality of groove-shaped common openings 241 and a communication liquid passage 242 that enables liquid movement between the common openings 241. A recess 21f may be formed at a location.

As illustrated in FIG. 5A, the plate member 240 may have a drain passage 243 in the common opening 241. The common opening 241 has drain passages 243 at both ends in the longitudinal direction. The recess 21f is opened at the bottom of the common opening 241. The bottom surface of the common opening 241, the bottom surface of the communication liquid passage 242, and the bottom surface of the drainage passage 243 are flush with each other.

As illustrated in FIG. 5(c), when the lid main body 331 of the lid member 330 is brought into contact with the upper surface 21a of the plate member 240, the protrusion 332 is inserted into the recess 21f, and the projection 332 is inserted into the opening 21g of the recess 21f. A clearance 21o is secured between the protrusion 332 and the protrusion 332. The protrusion 332 passes through the common opening 241 and reaches the recess 21f.

When the lid main body 331 of the lid member 330 is brought into contact with the upper surface 21a of the plate member 240, a portion of each of the plurality of protrusions 332 of the lid member 330 that protrudes from the contact portion 31 is in the recess 21f. inserted. The volumes of the portions of the plurality of protrusions 332 of the lid member 330 that are inserted into the recesses 21f are made equal to each other.

FIG. 5(b) is a top view of the plate member 240 shown in FIG. 5(a).

The lidded container 10E illustrated in FIG. 5 can be used to control the liquid level similarly to the lidded container 10A. First, the cells 2 are adhered to the bottom surface 21i of the recess 21f, and then the medium 1 is in an amount that fills the entire interior of the recess 21f and embeds some or all of the common opening 241, the communication fluid passage 242, and the drain passage 243. is injected into the recess 21f.

Next, the protrusion 332 of the lid member 330 is inserted into the recess 21f through the common opening 241, and the lower surface 31a of the contact portion 31 of the lid member 330 is brought into contact with the upper surface 21a of the plate member 240.
By inserting the protrusion 332 into the recess 21f, a part of the culture medium 1 in the recess 21f passes through the clearance 21o between the opening 21g of the recess 21f and the protrusion 332, and passes through the common opening 241 and the communication fluid passage 242. Or it is pushed out to the drain passage 243.

Next, the tip of the liquid suction pipe 91 of the liquid suction device 90 is brought into contact with the bottom surface 21n of the end of one or more liquid drainage passages 243, and the inside of the common opening 241, the communication liquid passage 242, and the liquid drainage passage 243 is removed. Aspirate and remove all of the medium 1.
In this lidded container 10E, even if the protrusion 332 of the lid member 330 is inserted into the recess 21f of the plate member 240, the connection between the portions on both sides and the communication liquid passage 242 is via the recess 21f in the common opening 241. Stay connected. Therefore, all the culture medium 1 inside the common opening 241 and the communication liquid passage 242 can be removed by suction using the liquid suction device 90 in which the tip of the liquid suction pipe 91 is disposed at the end of one liquid drainage passage 243 .

Next, when the lid member 330 is moved upward and removed from the plate member 240, the liquid level of the culture medium 1 in each recess 21f of the plate member 240 becomes equal in height.

In the lidded container 10E of FIG. 5, the protrusion 332 of the lid member 330 is inserted into the recess 21f, and the culture medium 1 overflowing from the recess 21f is transferred to the common opening 241, the connecting liquid passage 242 and the connecting liquid passage 242. Liquid can be moved between the drain passages 243.

The bottom surfaces of the common opening 241, the communicating fluid passage 242, or the draining fluid passage 243 are flush with each other. Therefore, all the culture medium 1 in the common opening 241, the communication liquid passage 242, or the drainage passage 243 can be removed by suction at the bottom surface 21n of the end of one or more drainage passages 243.

The bottom surface 21n of the end of the liquid drain passage 243 is used for height positioning for positioning the tip of the liquid suction pipe 91 in the height direction with respect to the plate member 240 by abutting the tip of the liquid suction pipe 91 of the liquid suction device 90. fulfill the role of the department.

The plate-like member 250 illustrated in FIG. 6A differs from the plate-like member 240 only in that the recessed portion is changed. When the lid main body 331 of the lid member 330 is brought into contact with the upper surface 21a of the plate member 250, a clearance is ensured between the protrusion 332 and the inner peripheral surface of the common opening 241.

The container 10F with a lid shown in FIG. 6 allows the height of the liquid level in the recess 251 to be made the same as in the case where the container 10E with a lid shown in FIG. 5 is used.

The protrusion 332 liquid-tightly seals the opening of the recess 251 by being in close contact with the entire circumference of a part of the inner peripheral surface of the opening of the recess 251 in the axial direction. The structure may include a sealing part.

The plate-like member 260 illustrated in FIGS. 7(a) to (c) has the common opening 21b of the plate-like member 210 in FIG. 1 changed to the common opening 262 in FIG. The recess 21f is replaced with a recess 264 in FIG. 7.

As illustrated in FIGS. 7A to 7C, the lidded container 10G includes a plate member 260 in which a plurality of common openings 262 and a recess 21f are formed, a lid main body 341, and a protrusion 343. It has a lid member 340.

The plurality of common openings 262 are open to the upper surface 261, and the recesses 264 are formed by being depressed from the bottom surface 263 of each of the common openings 262.

The protrusion 343 of the lid member 340 protrudes from the lower surface 342 of the lid main body 341, is inserted into the common opening 262, and is disposed on the plate member 260. When the protrusion 343 is inserted into the common opening 262 and brought into contact with the bottom surface 263 of the plate-like member 260, the protrusion 343 is brought into close contact with the bottom surface 263 of the common opening 262 and opens into the bottom surface 263 of the common opening 262. The opening 265 is closed.

The lidded container 10G illustrated in FIGS. 7(a) to (c) can be used to control the liquid level similarly to the lidded container 10A. First, an amount of culture medium 1 is injected to fill the entire interior of the recess 264 of the plate-shaped member 260 and to embed part or all of the common opening 262 .

Next, when the protrusion 343 is inserted into the recess 264 and brought into close contact with the common opening 262, the culture medium 1 overflows onto the upper surface 261 of the plate member 260. When the lid member 340 is removed from the plate member 260, the liquid level of the culture medium 1 in the recess 264 is aligned with the bottom surface 263 of the common opening 262.

The plate-shaped members 210 to 260 described so far are constructed by suctioning and removing the culture medium 1 from the common opening using a liquid suction device 90 in which the tip of the liquid suction pipe 91 is brought into contact with the bottom surface of the common opening from above. For each section, it is possible to align the liquid level of the culture medium 1 in all the recesses opening at the bottom of the common opening to the bottom of the common opening.
Note that in this specification, the plate-like member will be described with its thickness direction (direction perpendicular to the top surface of the plate-like member) as the height direction (hereinafter also referred to as plate height direction).

The plate member 260 in FIG. 8(a) is configured to suck and remove the culture medium 1 in the common opening 262 using a liquid suction device 90 in which the tip of a liquid suction pipe 91 is brought into contact with the bottom surface 263 of the common opening 262 from above. Thus, the liquid level of the culture medium 1 in the recess 264 can be aligned with the bottom surface 263 of the common opening 262.
The bottom surface 263 of the common opening 262 of the plate-like member 260 serves as a height positioning portion for positioning the tip of the liquid suction pipe 91 of the liquid suction device 90 abutted from above at the upper end of the recess 264 in the plate height direction. Fulfill.

The plate member 270 in FIG. 8(b) has a configuration in which the liquid drain passage 21j and the liquid absorber insertion hole 21m are omitted from the plate member 210 in FIGS. 1(a) to (c).
In this plate-like member 270, a liquid suction device is used in which the tip of the liquid suction pipe 91 is brought into contact with the bottom surface 21c of the common opening 21b from above to absorb the portion of the culture medium 1 dispensed into the recess 21f that overflows into the common opening 21b. 90 can be removed by suction. As a result, in this plate-shaped member 270, the culture medium 1 in the common opening 21b is suctioned and removed by the liquid suction device 90 in which the tip of the liquid suction pipe 91 is brought into contact with the bottom surface 21c of the common opening 21b from above. , the liquid level of the culture medium 1 in all the recesses 21f opening to the bottom surface 21c of the common opening 21b can be made equal to the bottom surface 21c of the common opening 21b.
The bottom surface 21c of the common opening 21b of the plate-like member 270 serves as a height positioning portion for positioning the tip of the liquid suction pipe 91 of the liquid suction device 90 that is abutted from above at the upper end of the recess 21f in the plate height direction. Fulfill.

In the plate member 270 of FIG. 8(b), aligning the liquid level of the culture medium 1 in each recess 21f with the bottom surface 21c of the common opening 21b means, for example, that each recess 21f opens to the bottom surface 21c of the common opening 21b. It can be used when reagents having the same concentration, composition, etc., are applied to cells 2 in the same cell. In this case, the culture medium 1 in the common opening 21b is suctioned and removed by a liquid suction device 90 in which the tip of a liquid suction pipe 91 is brought into contact with the bottom surface 21c of the common opening 21b, and the medium 1 is opened in the bottom surface 21c of the common opening 21b. After aligning the liquid level of the medium 1 in all the recesses 21f with the bottom surface 21c of the common opening 21b, the reagent is injected into each recess 21f, and the medium 1 and the reagent are injected into each recess 21f and the common opening 21b. The mixture is filled and the reagent is applied to the cells 2.

FIGS. 9A and 9B show an example of a height positioning section.
9A and 9B, the height positioning parts 281 and 282 are connected to the opening 21g of the recess 21f of the bottom surface 21c of the common opening 21b of the plate member 270 in FIG. An example is shown in which the method is applied to the area adjacent to the area. The height positioning parts 281 and 282 in FIGS. 9A and 9B are respectively formed in a part of the circumferential direction of the recess 21f in a region adjacent to the opening 21g of the recess 21f on the bottom surface 21c of the common opening 21b. ing.

The height positioning parts 281 and 282 in FIGS. 9(a) and 9(b) can also be applied to the bottom surface 21c of the common opening 21b of the plate-like member 260 in FIG. 8(a).
Moreover, the height positioning parts 281 and 282 in FIGS. 9(a) and 9(b) are common to the upper surface of the plate-shaped members of various embodiments such as the plate-shaped members 260 and 270 in FIGS. 11(a) and 11(b). It is also possible to provide the area adjacent to the opening.

The height positioning part 281 in FIG. 9(a) is a colored part formed in the area adjacent to the opening 21g of the recess 21f on the bottom surface 21c of the common opening 21b of the plate-like member 270 in FIG. 8(b). . The height positioning portion 281 may be formed by, for example, coloring a portion of the bottom surface 21c of the common opening 21b in a color different from the other area of the bottom surface 21c by contacting with a strong acid or a strong alkali.
This height positioning portion 281 serves as a mark indicating the abutting position of the tip of the liquid suction pipe 91 of the liquid suction device 90 on the bottom surface 21c of the common opening 21b. The height positioning portion 281 is formed with high precision alignment so that the position in the plate height direction matches the upper end of the recess 21f. By abutting the tip of the liquid suction pipe 91 of the liquid suction device 90 from above against the height positioning portion 281, it is positioned with high precision so as to coincide with the upper end of the recess 21f in the plate height direction.

The height positioning portion 282 in FIG. 9(b) is formed in the plate member 270 in FIG. 8(b) by being depressed from the bottom surface 21c of the common opening 21b and facing the recess 21f (opening in the recess 21f). This refers to the vicinity of the notch 282a. The height positioning portion 282 in FIG. 9(b) includes a cutout portion 282a.
The notch 282a is formed in the bottom surface 21c of the common opening 21b in the shape of a groove extending from the recess 21f with a groove width that does not allow insertion of the tip of the liquid suction pipe 91 of the liquid suction device 90. The notch 282a is formed, for example, in the shape of a groove whose width is narrower than the outer diameter of the circular cross-sectional tip of the liquid suction pipe 91 of the liquid suction device 90.

The area near the notch 282a on the bottom surface 21c of the common opening 21b is hereinafter also referred to as a support surface 282b. The height positioning portion 282 in FIG. 9(b) includes a support surface 282b.
The height positioning part 282 in FIG. 9(b) positions the tip of the liquid suction pipe 91 of the liquid suction device 90, which is abutted against the support surface 282b from above, at the same position as the upper end of the recess 21f in the plate height direction. . In addition, the liquid suction device 90 can be opened by abutting the tip of the liquid suction pipe 91 against the support surface 282b such that the liquid suction flow path inside thereof communicates with the notch 282a of the height positioning part 282. The culture medium 1 in the portion 21b can be smoothly removed by suction through the cutout portion 282a.
Furthermore, the notch 282a of the height positioning section 282 also serves as a mark indicating the position of the height positioning section 282.

FIGS. 10A and 10B show the height of the bottom surface of a notch 283 that is recessed from the bottom surface 21c of the common opening 21b of the plate member 270 and is formed facing the recess 21f (opening in the recess 21f). This is a positioning portion 283a. The height positioning portion 283a is formed on a flat surface of the plate member that extends perpendicularly to the plate height direction.

The notch 283 is formed in a size that allows the liquid suction pipe 91 of the liquid suction device 90 to be inserted from above the bottom surface 21c of the common opening 21b and the tip of the liquid suction pipe 91 to be butted against the height positioning portion 283a. There is.
The height positioning portion 283a is formed in a size that allows the tip of the liquid suction pipe 91 of the liquid suction device 90 to abut against it.

The height positioning part 283a, which is the bottom surface of the cutout part 283 shown in FIGS. It is positioned at a position that is shifted downward from the bottom surface 21c of the portion 21b.
In the configuration shown in FIGS. 10(a) and 10(b), the culture medium 1 in the recess 21f is sucked by the liquid suction device 90 in which the tip of the liquid suction pipe 91 is brought into contact with the height positioning part 283a which is the bottom surface of the notch 283. By doing so, the position of the liquid level of the culture medium 1 in the recessed portion 21f in the plate height direction can be aligned with the height positioning portion 283a. The formation position of the height positioning portion 283a, which is the bottom surface of the notch portion 283, in the plate height direction can be appropriately set at a position between the upper end in the depth direction and the lower end in the depth direction of the recess.
Further, the cutout portion 283a also serves as a mark indicating the position of the height positioning portion 283a, which is the bottom surface of the cutout portion 283a.

The cutout portion 283 and height positioning portion 283a in FIGS. 10(a) and 10(b) can also be applied to the bottom surface 21c of the common opening 21b of the plate-like member 260 in FIG. 8(a).
Furthermore, the cutout portion 283 and the height positioning portion 283a in FIGS. 10(a) and 10(b) are the upper surfaces of plate-like members of various embodiments such as the plate-like members 260 and 270 in FIGS. 11(a) and 11(b). It is also possible to form it in the area adjacent to the common opening of.

In the configuration shown in FIGS. 9(a) and 9(b) in which the height positioning portion is formed in the area adjacent to the common opening on the top surface of the plate-like member, the height positioning portion may be formed on the top surface of the plate-like member due to overflow from the common opening. The existing culture medium 1 is suctioned and removed by a liquid suction device 90 in which the tip of a liquid suction pipe 91 is brought into contact with the height positioning part from above, and the position of the medium liquid level in the common opening in the plate height direction is adjusted to the height. It can be aligned with the positioning part.
In the configuration shown in FIGS. 10(a) and 10(b) in which the height positioning portion is formed in the area adjacent to the common opening on the upper surface of the plate-like member, the culture medium 1 in the common opening is placed in the height positioning portion from above. By suctioning with the liquid suction device 90 against which the tip of the liquid suction pipe 91 is abutted, the position of the medium liquid level in the common opening in the plate height direction can be aligned with the height positioning portion.

The liquid injected into the recess is not limited to the medium, and instead of the medium, for example, a buffer, a reagent, or the like may be used. For example, carbohydrates such as proteins, lipids, nucleic acids, monosaccharides, and polysaccharides can be accommodated in the recesses.

The present invention includes the following aspects.
[1] A plate-like member having a plurality of recesses and a common opening whose bottom face is an end face on the opening side of at least two or more of the recesses, and a plate-like member having a common opening on the bottom face of the common opening of the plate-like member. A lid characterized in that it is made up of a lid member that includes a contact portion that is formed to be able to come into contact with each other, and a plurality of protrusions that are arranged to face the recess and protrude beyond the contact portion. Container with.
[2] The protrusion of the lid member is formed to be capable of liquid-tightly sealing the opening of the recess when the contact portion is brought into contact with the bottom surface of the common opening of the plate member. The container with a lid according to [1].
[3] When the contact portion of the lid member is brought into contact with the bottom surface of the common opening of the plate member, a clearance is ensured between the entire inner peripheral surface of the recess and the protrusion. The container with a lid according to [1].
[4] The plate-like member has a drain passage that communicates with the common opening, through which the liquid discharged from the recess can flow through the common opening when the protrusion of the lid member is inserted into the recess. The lidded container according to any one of [1] to [3], which is formed by
[5] A plate-shaped member that is formed into a plate shape and has a common opening that opens on one side of the upper surface, and a recess that is depressed from the bottom surface of the common opening, and the upper surface of the plate-shaped member. a lid member including a lid body placed on the lid body, the lid member protruding from the lid body and a lower surface of the lid body that abuts the upper surface of the plate member of the lid body; and a protrusion that is inserted into the recess through the common opening when the lower surface is brought into contact with the upper surface of the plate-shaped member.
[6] A plurality of groove-shaped common openings are formed in parallel in the plate-shaped member, and liquid can be transferred between the common openings adjacent to each other in the plate-shaped member. The lidded container according to [5], wherein a communication liquid passage is formed, and the recesses are formed at a plurality of locations in the extending direction of each of the groove-shaped common openings.
[7] A plate-shaped member formed in a plate-shape and having a plurality of common openings opened on one upper surface thereof and a recessed part recessed from the bottom surface of each of the common openings, and the plate-shaped member a lid member including a lid body disposed on the upper surface of the lid body, the lid member facing the plate member when placed on the lid body and the plate member of the lid body. a protrusion protruding from a lower surface and inserted into the common opening; the protrusion is inserted into the common opening of the plate-shaped member and brought into contact with the bottom surface of the common opening; A container with a lid that is brought into close contact with the entire inner surface of the common opening and closes an opening of the recess that opens at the bottom of the common opening.
[8] The lidded container according to any one of [1] to [7], wherein the bottom surface of at least one of the recesses is provided with cell adhesive properties.
[9] The lidded container according to any one of [1] to [8], characterized in that cells are adhered to at least one or more of the recesses.
[10] A common opening opening on one top surface of the plate-shaped body and one or more recesses recessed from the bottom surface of the common opening are formed, and the recess on the bottom surface of the common opening a height positioning portion for positioning the tip of the liquid suction pipe in the depth direction of the recess by bringing the tip of the liquid suction pipe of the liquid suction device into contact with a region adjacent to the opening from above; and the height positioning unit. A visible mark indicating the position of the recess is formed, and the height positioning part is located at the upper end of the recess in the depth direction or at a position between the upper end and the lower end of the recess in the depth direction. A plate-shaped member that extends vertically and forms a support surface that supports the tip of the liquid suction pipe.
[11] The plate according to [10], wherein the height positioning portion includes a notch formed in a depression from the bottom surface of the common opening and facing the common opening, and the notch also serves as the mark. shaped member.
[12] Maintaining the state in which the tip of the liquid suction pipe of the liquid suction device is abutted against the height positioning portion of the plate-shaped member according to [10] or [11] from above, and the liquid suction device A method for adjusting a liquid level height, wherein the liquid level in the recess is suctioned by the recess to align the position of the liquid level in the recess in the depth direction of the recess to the same level as the height positioning part.

1... Medium (sample), 2... Cell (sample), 10A... Container with lid, 10B... Container with lid, 10C... Container with lid, 10D... Container with lid, 10E... Container with lid, 10F... Container with lid, 10G...Container with lid, 21a...Top surface of plate-shaped member, 21b...Common opening, 21c...Bottom surface (of the common opening), 21d...Opening (of the common opening), 21e...Inner periphery (of the common opening) Surface, 21f...recess, 21g...opening (of the recess), 21h...inner peripheral surface (of the recess), 21i...bottom (of the recess), 21j...drainage passage, 21k...drainage bottom, 21m...liquid absorber Insertion hole, 21n... Height positioning part, 21o... Clearance, 31... Contact part, 31a... Lower surface (of the contact part), 31b... Side surface (of the contact part), 32... Projection, 90... Liquid absorber , 91...Liquid suction pipe, 210...Plate member, 220...Plate member, 221...Drain groove, 222...Groove bottom surface, 230...Plate member, 231...External discharge path, 232...Drain path bottom surface, 233... Drainage passage opening, 240... Plate member, 241... Common opening, 242... Communication liquid passage, 243... Drainage passage, 244... Bottom surface (of the common opening), 250... Plate member, 251... Recess, 260 ...Plate member, 261...Top surface of plate member, 262...Common opening, 263...Bottom surface (of common opening), 264...Recess, 265...Opening (of recess), 270...Plate member, 281...Height Positioning part (colored part), 282... Height positioning part, 282a... Notch, 282b... Support surface, 283... Notch, 283a... Height positioning part, support surface (bottom of the cutout), 310... Lid member , 320... Lid member, 321... Protrusion, 330... Lid member, 331... Lid body, 332... Protrusion, 340... Lid member, 341... Lid body, 342... Lower surface (of the lid body), 343... Protrusion

JP2008-29244A

Claims (7)

a plate-like member having a plurality of recesses and a common opening whose bottom surface is an end surface on the opening side of at least two of the recesses;
a contact part formed to be able to come into contact with the bottom surface of the common opening of the plate-like member; and a plurality of protrusions arranged to face the recess and protrude beyond the contact part. comprising: a lid member ;
A drain passage is formed in the plate-like member so as to communicate with the common opening, through which liquid discharged from the recess can flow through the common opening when the protrusion of the lid member is inserted into the recess. A container with a lid. The protrusion of the lid member is formed to be capable of liquid-tightly sealing the opening of the recess when the contact portion is brought into contact with the bottom surface of the common opening of the plate-shaped member. A container with a lid according to item 1. A clearance is secured between the entire inner circumferential surface of the recess and the protrusion when the contact portion of the lid member is brought into contact with the bottom surface of the common opening of the plate member. 1. The container with a lid according to 1. A plate-shaped member formed in a plate shape and having a common opening opening on one side of the upper surface, and a recessed part recessed from the bottom surface of the common opening;
a lid member including a lid body placed on the upper surface of the plate-like member;
The lid member protrudes from the lid body and a lower surface of the lid body that abuts the upper surface of the plate member, and when the lower surface of the lid body abuts the upper surface of the plate member. a protrusion inserted into the recess through the common opening ;
A plurality of the groove-shaped common openings are formed in parallel in the plate-like member,
A communication liquid passageway is formed between the common openings of the plate-shaped member to enable liquid movement between the mutually adjacent common openings,
The recessed portions are formed at a plurality of locations in the extending direction of each of the groove-shaped common openings . A plate-shaped member formed in a plate-shape and having a plurality of common openings opening on one top surface thereof, and a recessed part recessed from the bottom surface of each of the common openings;
a lid member including a lid body disposed on the upper surface of the plate-like member;
The lid member includes the lid main body and a protrusion that protrudes from a lower surface facing the plate member when placed on the plate member of the lid main body and is inserted into the common opening. ,
The protrusion is inserted into the common opening of the plate-shaped member and brought into contact with the bottom of the common opening, so that the protrusion is brought into close contact with the entire inner surface of the common opening, and the recess opens at the bottom of the common opening. close the opening of
A drain passage is formed in the plate-like member so as to communicate with the common opening, through which liquid discharged from the recess can flow through the common opening when the protrusion of the lid member is inserted into the recess. A container with a lid. The lidded container according to any one of claims 1 to 5, wherein the bottom surface of at least one of the recesses is provided with cell adhesive properties. The lidded container according to any one of claims 1 to 6 , characterized in that cells are adhered to at least one of the recesses.

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