JPH0588300U - Cell culture vessel - Google Patents

Abstract

(57) [Abstract] [Purpose] To provide a cell culture container having a pedestal that can stably fix the tip of a pipette or the tip of a liquid aspirator without touching the cell growth surface when aspirating the culture solution. .. [Claim] A culture container having a cell culture surface for growing cultured cells on a bottom surface of the container and containing a liquid medium therein, wherein a step is provided on at least a part of an inner surface of a side wall of the container, A cell culture container, characterized in that a base portion for locking and fixing the tip of a pipette or a liquid absorber is formed.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to a cell culture container used for cell culture in the fields of medicine and biology.

[0002]

[Prior Art]

 Research using cultured cells is expanding its range of applications, particularly in the fields of medicine and biology. Studies using cultured cells include, for example, preparation of monoclonal antibodies by the cell fusion method and introduction of foreign genes by the electroporation method.

When a large amount of cells are treated in a test tube and then the cells are cultured in a culture container to establish a target clone, as in the cell fusion method and gene transfer method, a selective medium is used. It is necessary to frequently exchange the used liquid. This liquid exchange is performed by suction with a Pasteur pipette or micropipette, or by using an automatic machine.

FIG. 7 shows the above-mentioned state, and is a cross-sectional view of the conventional cell culture container 1 when the suction operation of the culture solution is performed. As shown in FIG. 7, in the conventional method, there is a risk of sucking the cultured cells 10 themselves when sucking the culture solution. In particular, such a danger is remarkable near the cell growth surface 2. To avoid this risk, the tip of the pipette needs to be adjusted so that it does not touch the cell growth surface. However, such adjustment is very difficult, and the liquid exchange itself becomes incomplete, or the culture cell 10 itself is aspirated to cause a cell-deficient site 5 as shown in FIG. Sometimes.

[0005]

[Problems to be solved by the device]

 The present invention was made to overcome the above-mentioned drawbacks of the conventional method. The purpose of the present invention is to stably aspirate the tip of the pipette or the liquid during liquid aspiration without touching the cell growth surface. It is to provide a cell culture container having a pedestal to which the tip of the machine can be fixed.

[0006]

[Means for Solving the Problems]

 The above-mentioned problem is a culture container having a cell culture surface for growing cultured cells on the bottom surface of the container, and a liquid medium is contained therein. And a pedestal for locking and fixing the tip of a pipette or a liquid absorber are formed.

The pedestal of the present invention includes wells of a multi-plate, multi-plate, flat-bottomed container used for cell fusion and gene transfer, and a porous U-shaped container (used for cloning cytotoxic T cells). It can also be installed on a U-bottom multi-plate). In this case, the pedestal is preferably installed on the same side of each well.

[0008]

[Action]

 In the above structure, when the tip of the pipette or the automatic liquid suction machine is abutted against the table formed on the inner surface of the side wall of the container, the tip is locked and fixed to the table. Therefore, it is possible to perform liquid aspiration and liquid exchange without aspirating the cultured cells. In the case of multi-plate, fix the tip of the multi-pipette tip to the pedestal installed in each well and perform liquid suction.

[0009]

【Example】

 Example 1

FIG. 2 is a sectional view of a cell culture container 1 according to an embodiment of the present invention, and FIG. 3 is a plan view thereof. Further, FIG. 1 shows a usage state of the cell culture container 1. As shown in the figure, the culture container 1 has a substantially cylindrical shape, and the inner bottom surface thereof constitutes a cell growth surface 2 for growing the culture cell 10. The inside of the culture container 1 is filled with the liquid medium 3, and the cultured cells 10 are grown in a state of being in contact with the cell growth surface 2. A step 6 is provided on a part of the side wall of the container 1 at a position separated from the cell growth surface 2 by a predetermined distance. As a result, as clearly shown in FIG. 3, a base portion 7 is formed on the inner side surface of the container 1 at a position corresponding to the step portion 6. The base 7 is formed with a sufficient width so that the tip of the pipette 4 or the automatic suction device abuts on the base and is locked and fixed. Further, the height of the base 7 is set so that the liquid medium 3 can be sufficiently exchanged with a pipette or the like, and the suction of the cultured cells 10 can be suppressed to a minimum.

The cell culture container 1 of the above embodiment is used as shown in FIG. First, the cultured cells 10 are propagated on the cell growth surface 3 of the container 1 filled with the liquid medium 3. Next, when the liquid medium 3 needs to be replaced, the medium 3 is aspirated while the pipette 4 or the tip of the automatic suction machine is in contact with the base 7 (step 6), and the liquid is drained. To do. After sufficient drainage is completed, a new liquid medium 3 may be replenished. If the pipette 4 is drained while the tip of the pipet 4 is in contact with the base 7, the tip of the pipette or the like is locked and fixed to the base, and the cell growth surface 2 does not come too close. Therefore, it is possible to perform the liquid exchange accurately and quantitatively without sucking the cultured cells 10. Example 2

4 and 5 show a second embodiment of the present invention. In this embodiment, the present invention is applied to a flat-bottomed multi-plate 20 (perforated flat-bottomed container) used for cell fusion and gene transfer. As is clear from the figure, in this embodiment, each well of the multi-plate 20 has a structure similar to that of the embodiment of FIG. 1 and has a step 6 (a base 7).

Therefore, as shown in FIG. 4, by making each tip end of the multi-pipette 4 abut on the base of the well, the level of the tip end of each well can be fixed. . If the medium 3 is sucked in this state, suction of the cultured cells 10 can be prevented and the drainage operation can be performed in all the wells. The state after the liquid medium 3 is drained in this way is shown in FIG. Since the tip of the pipette 4 in each well can be fixed at a fixed level, the residual amount of the medium 3 can be made constant for all wells as shown in the figure. As a result, even when the medium 3 is subsequently replenished, the concentration of each component in the replenished medium can be maintained constant in all wells. Example 3

FIG. 4 shows an embodiment in which the present invention is applied to a porous U-shaped container (U-bottom multi-plate). Such a U-bottomed multiplate is used, for example, for cloning cytotoxic T cells. As is clear from the figure, this embodiment is the same as the embodiment of FIGS. 4 and 5 except that the bottom surface of each well has a U-shaped cross section, and each well has a step 6 ( Since the base 7) is provided, the same effect as that of the embodiment shown in FIGS. 4 and 5 can be obtained.

That is, even when the cell growth surface is not flat as in this embodiment, the tip of each tip of the multi-pipette 4 is brought into contact with the base of each well to minimize damage to the cultured cells 10. The liquid exchange can be performed accurately and quantitatively.

[0016]

[Effect of the device]

 As described in detail above, according to the present invention, the following effects can be obtained by providing a step at one end of the cell growth surface in the culture container and installing the base. 1) No aspiration of cultured cells. 2) Since the liquid exchange can be performed quantitatively and accurately, the exchange amount of the selective medium becomes constant. 3) Since the suction operation with a pipette can be performed stably, the liquid exchange operation can be performed easily and quickly.

As a result, experimental operations such as cell fusion and gene transfer can be appropriately continued. In particular, when the present invention is applied to a porous container, the concentration of the selected drug becomes constant, so that the gene transfer conditions are set accurately.

[Brief description of drawings]

FIG. 1 is a sectional view of a culture solution suction operation in a cell culture container of the present invention.

FIG. 2 is a sectional view of the cell culture container of the present invention.

FIG. 3 is a plan view of the cell culture container of the present invention.

FIG. 4 is a cross-sectional view of a porous container comprising the cell culture container of the present invention before liquid suction.

FIG. 5 is a cross-sectional view of a porous container comprising the cell culture container of the present invention after liquid suction.

FIG. 6 is a cross-sectional view of a U-bottomed multi-plate comprising the cell culture container of the present invention.

FIG. 7 is a cross-sectional view of a culture solution suction operation in a conventional cell culture container.

FIG. 8 is a plan view of a cell growth surface after sucking a culture solution in a conventional cell culture container.

[Explanation of symbols]

1 ... Cell culture vessel, 2 ... Cell growth surface (bottom of vessel), 3 ...
Culture solution, 4 ... Pipette, multi-pipette, or automatic aspirator, 5 ... Cell loss site, 6 ... Step, 7 ... Stand, 10 ...
Cultured cells, 20 ... Flat bottom multiplate, 30 ... U bottom multiplate

Claims (1)

[Scope of utility model registration request] 1. A culture container having a cell culture surface for growing cultured cells on the bottom surface of the container and containing a liquid medium therein, wherein a step is formed on at least a part of an inner surface of a side wall of the container. A cell culture container provided with a base portion for locking and fixing the tip of a pipette or a liquid absorber.