JP2020516281A5 - - Google Patents
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- Publication number
- JP2020516281A5 JP2020516281A5 JP2019555645A JP2019555645A JP2020516281A5 JP 2020516281 A5 JP2020516281 A5 JP 2020516281A5 JP 2019555645 A JP2019555645 A JP 2019555645A JP 2019555645 A JP2019555645 A JP 2019555645A JP 2020516281 A5 JP2020516281 A5 JP 2020516281A5
- Authority
- JP
- Japan
- Prior art keywords
- adapter
- stranded
- double
- overhang
- polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims description 54
- 239000002773 nucleotide Substances 0.000 claims description 45
- 125000003729 nucleotide group Chemical group 0.000 claims description 45
- 150000007523 nucleic acids Chemical class 0.000 claims description 41
- 102000039446 nucleic acids Human genes 0.000 claims description 38
- 108020004707 nucleic acids Proteins 0.000 claims description 38
- 108020004414 DNA Proteins 0.000 claims description 34
- 102000053602 DNA Human genes 0.000 claims description 22
- 230000001915 proofreading effect Effects 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 10
- 238000012163 sequencing technique Methods 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 4
- 238000007481 next generation sequencing Methods 0.000 claims description 4
- 108010017826 DNA Polymerase I Proteins 0.000 claims description 3
- 102000004594 DNA Polymerase I Human genes 0.000 claims description 3
- 108091034117 Oligonucleotide Proteins 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 3
- 206010028980 Neoplasm Diseases 0.000 claims description 2
- 230000003321 amplification Effects 0.000 claims description 2
- 238000000137 annealing Methods 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 210000001124 body fluid Anatomy 0.000 claims description 2
- 239000010839 body fluid Substances 0.000 claims description 2
- 201000011510 cancer Diseases 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 210000004602 germ cell Anatomy 0.000 claims description 2
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 230000035772 mutation Effects 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- 210000002381 plasma Anatomy 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 claims description 2
- 210000001082 somatic cell Anatomy 0.000 claims description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022016339A JP7514263B2 (ja) | 2017-04-14 | 2022-02-04 | 試料核酸にアダプターを付着する方法 |
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762485769P | 2017-04-14 | 2017-04-14 | |
| PCT/US2017/027809 WO2017181146A1 (en) | 2016-04-14 | 2017-04-14 | Methods for early detection of cancer |
| US62/485,769 | 2017-04-14 | ||
| USPCT/US2017/027809 | 2017-04-14 | ||
| US201762486663P | 2017-04-18 | 2017-04-18 | |
| US62/486,663 | 2017-04-18 | ||
| US201762517145P | 2017-06-08 | 2017-06-08 | |
| US62/517,145 | 2017-06-08 | ||
| PCT/US2018/027632 WO2018191702A2 (en) | 2017-04-14 | 2018-04-13 | Methods of attaching adapters to sample nucleic acids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022016339A Division JP7514263B2 (ja) | 2017-04-14 | 2022-02-04 | 試料核酸にアダプターを付着する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020516281A JP2020516281A (ja) | 2020-06-11 |
| JP2020516281A5 true JP2020516281A5 (enExample) | 2021-05-20 |
| JP7046097B2 JP7046097B2 (ja) | 2022-04-01 |
Family
ID=63792821
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019555645A Active JP7046097B2 (ja) | 2017-04-14 | 2018-04-13 | 試料核酸にアダプターを付着する方法 |
| JP2022016339A Active JP7514263B2 (ja) | 2017-04-14 | 2022-02-04 | 試料核酸にアダプターを付着する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022016339A Active JP7514263B2 (ja) | 2017-04-14 | 2022-02-04 | 試料核酸にアダプターを付着する方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20200131567A1 (enExample) |
| EP (2) | EP3610032B1 (enExample) |
| JP (2) | JP7046097B2 (enExample) |
| CN (1) | CN110546272B (enExample) |
| AU (1) | AU2018252018A1 (enExample) |
| CA (1) | CA3057163A1 (enExample) |
| ES (2) | ES2962223T3 (enExample) |
| HU (1) | HUE063675T2 (enExample) |
| PL (1) | PL3885445T3 (enExample) |
| WO (1) | WO2018191702A2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3561072A1 (en) | 2012-12-10 | 2019-10-30 | Resolution Bioscience, Inc. | Methods for targeted genomic analysis |
| US20160053301A1 (en) | 2014-08-22 | 2016-02-25 | Clearfork Bioscience, Inc. | Methods for quantitative genetic analysis of cell free dna |
| EP3889257A1 (en) | 2015-11-11 | 2021-10-06 | Resolution Bioscience, Inc. | High efficiency construction of dna libraries |
| EP4488686A3 (en) | 2016-04-14 | 2025-04-30 | Guardant Health, Inc. | Methods for early detection of cancer |
| AU2017315769B2 (en) | 2016-08-25 | 2024-02-01 | Resolution Bioscience, Inc. | Methods for the detection of genomic copy changes in DNA samples |
| CN116144735A (zh) * | 2019-08-12 | 2023-05-23 | 深圳市真迈生物科技有限公司 | 核酸样本处理方法、测序方法和试剂盒 |
| WO2021067484A1 (en) * | 2019-09-30 | 2021-04-08 | Guardant Health, Inc. | Compositions and methods for analyzing cell-free dna in methylation partitioning assays |
| JP2022553164A (ja) * | 2019-10-25 | 2022-12-22 | ガーダント ヘルス， インコーポレイテッド | ３’オーバーハングを修復する方法 |
| KR20230065295A (ko) * | 2020-09-08 | 2023-05-11 | 레졸루션 바이오사이언스, 인크. | 유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법 |
| WO2022115608A1 (en) * | 2020-11-25 | 2022-06-02 | Alida Biosciences, Inc. | Multiplexed profiling of rna and dna modifications |
| EP4265721A4 (en) * | 2020-12-15 | 2024-11-13 | Genodive Pharma Inc. | METHOD FOR EVALUATING ADAPTER LIGATION EFFICIENCY IN A DNA SAMPLE SEQUENCE |
| WO2023023402A2 (en) * | 2021-08-20 | 2023-02-23 | Guardant Health, Inc. | Methods for simultaneous molecular and sample barcoding |
| EP4437125A1 (en) * | 2021-11-24 | 2024-10-02 | Alida Biosciences, Inc. | Rna and dna analysis using engineered surfaces |
| WO2024220475A1 (en) * | 2023-04-21 | 2024-10-24 | Twist Bioscience Corporation | Polymerase variants |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US20030017081A1 (en) | 1994-02-10 | 2003-01-23 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| AU2002359522A1 (en) | 2001-11-28 | 2003-06-10 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9096952B2 (en) * | 2010-06-24 | 2015-08-04 | Population Genetics Technologies Ltd. | Methods and compositions for polynucleotide library production, immortalization and region of interest extraction |
| US8829172B2 (en) * | 2011-03-11 | 2014-09-09 | Academia Sinica | Multiplex barcoded paired-end diTag (mbPED) sequencing approach and ITS application in fusion gene identification |
| US10023856B2 (en) * | 2013-09-25 | 2018-07-17 | Thermo Fisher Scientific Baltics Uab | Enzyme composition for DNA end repair, adenylation, phosphorylation |
| ES2912183T3 (es) * | 2013-12-30 | 2022-05-24 | Atreca Inc | Análisis de ácidos nucleicos asociados a células individuales utilizando códigos de barras de ácidos nucleicos |
| WO2016019360A1 (en) * | 2014-08-01 | 2016-02-04 | Dovetail Genomics Llc | Tagging nucleic acids for sequence assembly |
| WO2016135300A1 (en) | 2015-02-26 | 2016-09-01 | Qiagen Gmbh | Efficiency improving methods for gene library generation |
-
2018
- 2018-04-13 HU HUE21157081A patent/HUE063675T2/hu unknown
- 2018-04-13 AU AU2018252018A patent/AU2018252018A1/en not_active Abandoned
- 2018-04-13 JP JP2019555645A patent/JP7046097B2/ja active Active
- 2018-04-13 CA CA3057163A patent/CA3057163A1/en active Pending
- 2018-04-13 ES ES21157081T patent/ES2962223T3/es active Active
- 2018-04-13 WO PCT/US2018/027632 patent/WO2018191702A2/en not_active Ceased
- 2018-04-13 EP EP18783709.1A patent/EP3610032B1/en active Active
- 2018-04-13 ES ES18783709T patent/ES2868074T3/es active Active
- 2018-04-13 PL PL21157081.7T patent/PL3885445T3/pl unknown
- 2018-04-13 CN CN201880025117.1A patent/CN110546272B/zh active Active
- 2018-04-13 EP EP21157081.7A patent/EP3885445B1/en active Active
-
2019
- 2019-10-08 US US16/596,180 patent/US20200131567A1/en not_active Abandoned
-
2020
- 2020-05-21 US US16/880,706 patent/US20200283839A1/en not_active Abandoned
-
2022
- 2022-02-04 JP JP2022016339A patent/JP7514263B2/ja active Active
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