CN110546272B - 将衔接子附接至样品核酸的方法 - Google Patents

将衔接子附接至样品核酸的方法 Download PDF

Info

Publication number
CN110546272B
CN110546272B CN201880025117.1A CN201880025117A CN110546272B CN 110546272 B CN110546272 B CN 110546272B CN 201880025117 A CN201880025117 A CN 201880025117A CN 110546272 B CN110546272 B CN 110546272B
Authority
CN
China
Prior art keywords
sample
adaptors
nucleic acid
stranded
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201880025117.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN110546272A (zh
Inventor
安德鲁·肯尼迪
斯特凡尼·安·沃德·莫蒂默
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guardant Health Inc
Original Assignee
Guardant Health Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2017/027809 external-priority patent/WO2017181146A1/en
Application filed by Guardant Health Inc filed Critical Guardant Health Inc
Publication of CN110546272A publication Critical patent/CN110546272A/zh
Application granted granted Critical
Publication of CN110546272B publication Critical patent/CN110546272B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201880025117.1A 2017-04-14 2018-04-13 将衔接子附接至样品核酸的方法 Active CN110546272B (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762485769P 2017-04-14 2017-04-14
PCT/US2017/027809 WO2017181146A1 (en) 2016-04-14 2017-04-14 Methods for early detection of cancer
US62/485,769 2017-04-14
USPCT/US2017/027809 2017-04-14
US201762486663P 2017-04-18 2017-04-18
US62/486,663 2017-04-18
US201762517145P 2017-06-08 2017-06-08
US62/517,145 2017-06-08
PCT/US2018/027632 WO2018191702A2 (en) 2017-04-14 2018-04-13 Methods of attaching adapters to sample nucleic acids

Publications (2)

Publication Number Publication Date
CN110546272A CN110546272A (zh) 2019-12-06
CN110546272B true CN110546272B (zh) 2024-06-07

Family

ID=63792821

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880025117.1A Active CN110546272B (zh) 2017-04-14 2018-04-13 将衔接子附接至样品核酸的方法

Country Status (10)

Country Link
US (2) US20200131567A1 (enExample)
EP (2) EP3610032B1 (enExample)
JP (2) JP7046097B2 (enExample)
CN (1) CN110546272B (enExample)
AU (1) AU2018252018A1 (enExample)
CA (1) CA3057163A1 (enExample)
ES (2) ES2962223T3 (enExample)
HU (1) HUE063675T2 (enExample)
PL (1) PL3885445T3 (enExample)
WO (1) WO2018191702A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
EP4488686A3 (en) 2016-04-14 2025-04-30 Guardant Health, Inc. Methods for early detection of cancer
AU2017315769B2 (en) 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
CN116144735A (zh) * 2019-08-12 2023-05-23 深圳市真迈生物科技有限公司 核酸样本处理方法、测序方法和试剂盒
WO2021067484A1 (en) * 2019-09-30 2021-04-08 Guardant Health, Inc. Compositions and methods for analyzing cell-free dna in methylation partitioning assays
JP2022553164A (ja) * 2019-10-25 2022-12-22 ガーダント ヘルス， インコーポレイテッド ３’オーバーハングを修復する方法
KR20230065295A (ko) * 2020-09-08 2023-05-11 레졸루션 바이오사이언스, 인크. 유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법
WO2022115608A1 (en) * 2020-11-25 2022-06-02 Alida Biosciences, Inc. Multiplexed profiling of rna and dna modifications
EP4265721A4 (en) * 2020-12-15 2024-11-13 Genodive Pharma Inc. METHOD FOR EVALUATING ADAPTER LIGATION EFFICIENCY IN A DNA SAMPLE SEQUENCE
WO2023023402A2 (en) * 2021-08-20 2023-02-23 Guardant Health, Inc. Methods for simultaneous molecular and sample barcoding
EP4437125A1 (en) * 2021-11-24 2024-10-02 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
WO2024220475A1 (en) * 2023-04-21 2024-10-24 Twist Bioscience Corporation Polymerase variants

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105722978A (zh) * 2013-09-25 2016-06-29 赛默飞世尔科技波罗的海有限公司 用于dna末端修复、腺苷酸化、磷酸化的酶组合物

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
AU2002359522A1 (en) 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9096952B2 (en) * 2010-06-24 2015-08-04 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US8829172B2 (en) * 2011-03-11 2014-09-09 Academia Sinica Multiplex barcoded paired-end diTag (mbPED) sequencing approach and ITS application in fusion gene identification
ES2912183T3 (es) * 2013-12-30 2022-05-24 Atreca Inc Análisis de ácidos nucleicos asociados a células individuales utilizando códigos de barras de ácidos nucleicos
WO2016019360A1 (en) * 2014-08-01 2016-02-04 Dovetail Genomics Llc Tagging nucleic acids for sequence assembly
WO2016135300A1 (en) 2015-02-26 2016-09-01 Qiagen Gmbh Efficiency improving methods for gene library generation

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105722978A (zh) * 2013-09-25 2016-06-29 赛默飞世尔科技波罗的海有限公司 用于dna末端修复、腺苷酸化、磷酸化的酶组合物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase;Yoshiyuki Ohtsubo等;Scientific Reports;第7卷;摘要和第5页最后一段 *

Also Published As

Publication number Publication date
JP7046097B2 (ja) 2022-04-01
EP3885445A1 (en) 2021-09-29
PL3885445T3 (pl) 2024-01-29
EP3610032A4 (en) 2020-05-06
JP7514263B2 (ja) 2024-07-10
US20200131567A1 (en) 2020-04-30
EP3610032A2 (en) 2020-02-19
EP3610032B1 (en) 2021-03-10
HUE063675T2 (hu) 2024-01-28
CA3057163A1 (en) 2018-10-18
JP2020516281A (ja) 2020-06-11
WO2018191702A3 (en) 2019-02-21
ES2868074T3 (es) 2021-10-21
JP2022048389A (ja) 2022-03-25
EP3885445B1 (en) 2023-08-23
WO2018191702A2 (en) 2018-10-18
CN110546272A (zh) 2019-12-06
AU2018252018A1 (en) 2019-10-10
US20200283839A1 (en) 2020-09-10
EP3885445C0 (en) 2023-08-23
ES2962223T3 (es) 2024-03-18

Similar Documents

Publication Publication Date Title
CN110546272B (zh) 将衔接子附接至样品核酸的方法
EP3177740B1 (en) Digital measurements from targeted sequencing
WO2019055715A1 (en) UNIVERSAL SHORT ADAPTERS WITH UNIQUE NON-RANDOM MOLECULAR IDENTIFIERS OF VARIABLE LENGTH
EP3532635B1 (en) Barcoded circular library construction for identification of chimeric products
CN105408496A (zh) 检测稀有突变和拷贝数变异的系统和方法
CN113227393B (zh) 用于校准表观遗传分区测定的方法、组合物和系统
US20240141425A1 (en) Correcting for deamination-induced sequence errors
EP4048812B1 (en) Methods for 3' overhang repair
HK40027193B (zh) 具有可变长度非随机独特分子标识符的通用短衔接子

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant