CN110546272B - 将衔接子附接至样品核酸的方法 - Google Patents

将衔接子附接至样品核酸的方法 Download PDF

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Publication number
CN110546272B
CN110546272B CN201880025117.1A CN201880025117A CN110546272B CN 110546272 B CN110546272 B CN 110546272B CN 201880025117 A CN201880025117 A CN 201880025117A CN 110546272 B CN110546272 B CN 110546272B
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sample
stranded
nucleic acid
adaptors
double
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CN110546272A (zh
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安德鲁·肯尼迪
斯特凡尼·安·沃德·莫蒂默
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Guardant Health Inc
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Guardant Health Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201880025117.1A 2017-04-14 2018-04-13 将衔接子附接至样品核酸的方法 Active CN110546272B (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762485769P 2017-04-14 2017-04-14
PCT/US2017/027809 WO2017181146A1 (en) 2016-04-14 2017-04-14 Methods for early detection of cancer
USPCT/US2017/027809 2017-04-14
US62/485,769 2017-04-14
US201762486663P 2017-04-18 2017-04-18
US62/486,663 2017-04-18
US201762517145P 2017-06-08 2017-06-08
US62/517,145 2017-06-08
PCT/US2018/027632 WO2018191702A2 (en) 2017-04-14 2018-04-13 Methods of attaching adapters to sample nucleic acids

Publications (2)

Publication Number Publication Date
CN110546272A CN110546272A (zh) 2019-12-06
CN110546272B true CN110546272B (zh) 2024-06-07

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CN201880025117.1A Active CN110546272B (zh) 2017-04-14 2018-04-13 将衔接子附接至样品核酸的方法

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US (2) US20200131567A1 (enExample)
EP (2) EP3610032B1 (enExample)
JP (2) JP7046097B2 (enExample)
CN (1) CN110546272B (enExample)
AU (1) AU2018252018A1 (enExample)
CA (1) CA3057163A1 (enExample)
ES (2) ES2868074T3 (enExample)
HU (1) HUE063675T2 (enExample)
PL (1) PL3885445T3 (enExample)
WO (1) WO2018191702A2 (enExample)

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Publication number Priority date Publication date Assignee Title
CN105531375B (zh) 2012-12-10 2020-03-03 分析生物科学有限公司 靶向基因组分析的方法
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
WO2017181146A1 (en) 2016-04-14 2017-10-19 Guardant Health, Inc. Methods for early detection of cancer
KR102505122B1 (ko) 2016-08-25 2023-03-08 레졸루션 바이오사이언스, 인크. Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법
CN116144735A (zh) * 2019-08-12 2023-05-23 深圳市真迈生物科技有限公司 核酸样本处理方法、测序方法和试剂盒
EP4041919A1 (en) * 2019-09-30 2022-08-17 Guardant Health, Inc. Compositions and methods for analyzing cell-free dna in methylation partitioning assays
EP4775688A2 (en) * 2019-10-25 2026-07-15 Guardant Health, Inc. Methods for 3' overhang repair
DK4211246T3 (da) * 2020-09-08 2025-08-18 Resolution Bioscience Inc Adaptorer og fremgangsmåder til højeffektiv konstruktion af genetiske biblioteker og genetisk analyse
EP4251760A1 (en) 2020-11-25 2023-10-04 Alida Biosciences, Inc. Multiplexed profiling of rna and dna modifications
WO2022131285A1 (ja) * 2020-12-15 2022-06-23 ジェノダイブファーマ株式会社 Ｄｎａサンプルのシーケンスにおけるアダプター結合効率を評価する方法
WO2023023402A2 (en) * 2021-08-20 2023-02-23 Guardant Health, Inc. Methods for simultaneous molecular and sample barcoding
WO2023097295A1 (en) * 2021-11-24 2023-06-01 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
KR20250175336A (ko) * 2023-04-21 2025-12-16 트위스트 바이오사이언스 코포레이션 중합효소 변이체

Citations (1)

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CN105722978A (zh) * 2013-09-25 2016-06-29 赛默飞世尔科技波罗的海有限公司 用于dna末端修复、腺苷酸化、磷酸化的酶组合物

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US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
ATE449186T1 (de) 2001-11-28 2009-12-15 Applied Biosystems Llc Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP2585593B1 (en) * 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
US8829172B2 (en) * 2011-03-11 2014-09-09 Academia Sinica Multiplex barcoded paired-end diTag (mbPED) sequencing approach and ITS application in fusion gene identification
JP6608368B2 (ja) * 2013-12-30 2019-11-20 アトレカ インコーポレイテッド 核酸バーコードを用いた単一細胞と関連づけられた核酸の分析方法
AU2015296029B2 (en) * 2014-08-01 2022-01-27 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
WO2016135300A1 (en) * 2015-02-26 2016-09-01 Qiagen Gmbh Efficiency improving methods for gene library generation

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CN105722978A (zh) * 2013-09-25 2016-06-29 赛默飞世尔科技波罗的海有限公司 用于dna末端修复、腺苷酸化、磷酸化的酶组合物

Non-Patent Citations (1)

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Publication number Publication date
JP7046097B2 (ja) 2022-04-01
HUE063675T2 (hu) 2024-01-28
US20200283839A1 (en) 2020-09-10
EP3885445C0 (en) 2023-08-23
JP2020516281A (ja) 2020-06-11
CN110546272A (zh) 2019-12-06
WO2018191702A3 (en) 2019-02-21
WO2018191702A2 (en) 2018-10-18
US20200131567A1 (en) 2020-04-30
PL3885445T3 (pl) 2024-01-29
AU2018252018A1 (en) 2019-10-10
JP2022048389A (ja) 2022-03-25
EP3610032A4 (en) 2020-05-06
CA3057163A1 (en) 2018-10-18
ES2962223T3 (es) 2024-03-18
EP3885445B1 (en) 2023-08-23
JP7514263B2 (ja) 2024-07-10
EP3610032B1 (en) 2021-03-10
ES2868074T3 (es) 2021-10-21
EP3610032A2 (en) 2020-02-19
EP3885445A1 (en) 2021-09-29

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