AU2018252018A1 - Methods of attaching adapters to sample nucleic acids - Google Patents

Methods of attaching adapters to sample nucleic acids Download PDF

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Publication number
AU2018252018A1
AU2018252018A1 AU2018252018A AU2018252018A AU2018252018A1 AU 2018252018 A1 AU2018252018 A1 AU 2018252018A1 AU 2018252018 A AU2018252018 A AU 2018252018A AU 2018252018 A AU2018252018 A AU 2018252018A AU 2018252018 A1 AU2018252018 A1 AU 2018252018A1
Authority
AU
Australia
Prior art keywords
adapters
sample
nucleic acids
stranded
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2018252018A
Other languages
English (en)
Inventor
Andrew Kennedy
Stefanie Ann Ward Mortimer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guardant Health Inc
Original Assignee
Guardant Health Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2017/027809 external-priority patent/WO2017181146A1/en
Application filed by Guardant Health Inc filed Critical Guardant Health Inc
Publication of AU2018252018A1 publication Critical patent/AU2018252018A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2018252018A 2017-04-14 2018-04-13 Methods of attaching adapters to sample nucleic acids Abandoned AU2018252018A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201762485769P 2017-04-14 2017-04-14
AUPCT/US2017/027809 2017-04-14
PCT/US2017/027809 WO2017181146A1 (en) 2016-04-14 2017-04-14 Methods for early detection of cancer
US62/485,769 2017-04-14
US201762486663P 2017-04-18 2017-04-18
US62/486,663 2017-04-18
US201762517145P 2017-06-08 2017-06-08
US62/517,145 2017-06-08
PCT/US2018/027632 WO2018191702A2 (en) 2017-04-14 2018-04-13 Methods of attaching adapters to sample nucleic acids

Publications (1)

Publication Number Publication Date
AU2018252018A1 true AU2018252018A1 (en) 2019-10-10

Family

ID=63792821

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2018252018A Abandoned AU2018252018A1 (en) 2017-04-14 2018-04-13 Methods of attaching adapters to sample nucleic acids

Country Status (10)

Country Link
US (2) US20200131567A1 (enExample)
EP (2) EP3610032B1 (enExample)
JP (2) JP7046097B2 (enExample)
CN (1) CN110546272B (enExample)
AU (1) AU2018252018A1 (enExample)
CA (1) CA3057163A1 (enExample)
ES (2) ES2962223T3 (enExample)
HU (1) HUE063675T2 (enExample)
PL (1) PL3885445T3 (enExample)
WO (1) WO2018191702A2 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3561072A1 (en) 2012-12-10 2019-10-30 Resolution Bioscience, Inc. Methods for targeted genomic analysis
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
EP3889257A1 (en) 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
EP4488686A3 (en) 2016-04-14 2025-04-30 Guardant Health, Inc. Methods for early detection of cancer
AU2017315769B2 (en) 2016-08-25 2024-02-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in DNA samples
CN116144735A (zh) * 2019-08-12 2023-05-23 深圳市真迈生物科技有限公司 核酸样本处理方法、测序方法和试剂盒
WO2021067484A1 (en) * 2019-09-30 2021-04-08 Guardant Health, Inc. Compositions and methods for analyzing cell-free dna in methylation partitioning assays
JP2022553164A (ja) * 2019-10-25 2022-12-22 ガーダント ヘルス, インコーポレイテッド 3’オーバーハングを修復する方法
KR20230065295A (ko) * 2020-09-08 2023-05-11 레졸루션 바이오사이언스, 인크. 유전자 라이브러리 및 유전자 분석의 고효율성 구축을 위한 어댑터 및 방법
WO2022115608A1 (en) * 2020-11-25 2022-06-02 Alida Biosciences, Inc. Multiplexed profiling of rna and dna modifications
EP4265721A4 (en) * 2020-12-15 2024-11-13 Genodive Pharma Inc. METHOD FOR EVALUATING ADAPTER LIGATION EFFICIENCY IN A DNA SAMPLE SEQUENCE
WO2023023402A2 (en) * 2021-08-20 2023-02-23 Guardant Health, Inc. Methods for simultaneous molecular and sample barcoding
EP4437125A1 (en) * 2021-11-24 2024-10-02 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
WO2024220475A1 (en) * 2023-04-21 2024-10-24 Twist Bioscience Corporation Polymerase variants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US20030017081A1 (en) 1994-02-10 2003-01-23 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
AU2002359522A1 (en) 2001-11-28 2003-06-10 Applera Corporation Compositions and methods of selective nucleic acid isolation
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9096952B2 (en) * 2010-06-24 2015-08-04 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US8829172B2 (en) * 2011-03-11 2014-09-09 Academia Sinica Multiplex barcoded paired-end diTag (mbPED) sequencing approach and ITS application in fusion gene identification
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
ES2912183T3 (es) * 2013-12-30 2022-05-24 Atreca Inc Análisis de ácidos nucleicos asociados a células individuales utilizando códigos de barras de ácidos nucleicos
WO2016019360A1 (en) * 2014-08-01 2016-02-04 Dovetail Genomics Llc Tagging nucleic acids for sequence assembly
WO2016135300A1 (en) 2015-02-26 2016-09-01 Qiagen Gmbh Efficiency improving methods for gene library generation

Also Published As

Publication number Publication date
JP7046097B2 (ja) 2022-04-01
EP3885445A1 (en) 2021-09-29
PL3885445T3 (pl) 2024-01-29
EP3610032A4 (en) 2020-05-06
JP7514263B2 (ja) 2024-07-10
US20200131567A1 (en) 2020-04-30
EP3610032A2 (en) 2020-02-19
EP3610032B1 (en) 2021-03-10
HUE063675T2 (hu) 2024-01-28
CA3057163A1 (en) 2018-10-18
JP2020516281A (ja) 2020-06-11
WO2018191702A3 (en) 2019-02-21
ES2868074T3 (es) 2021-10-21
JP2022048389A (ja) 2022-03-25
EP3885445B1 (en) 2023-08-23
WO2018191702A2 (en) 2018-10-18
CN110546272A (zh) 2019-12-06
CN110546272B (zh) 2024-06-07
US20200283839A1 (en) 2020-09-10
EP3885445C0 (en) 2023-08-23
ES2962223T3 (es) 2024-03-18

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MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted