JP2020147567A - Method for producing cosmetic composition containing perilla oil beauty oil and plant omega-3, and cosmetic composition containing perilla oil beauty oil and plant omega-3 as active ingredient - Google Patents
Method for producing cosmetic composition containing perilla oil beauty oil and plant omega-3, and cosmetic composition containing perilla oil beauty oil and plant omega-3 as active ingredient Download PDFInfo
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- JP2020147567A JP2020147567A JP2020043405A JP2020043405A JP2020147567A JP 2020147567 A JP2020147567 A JP 2020147567A JP 2020043405 A JP2020043405 A JP 2020043405A JP 2020043405 A JP2020043405 A JP 2020043405A JP 2020147567 A JP2020147567 A JP 2020147567A
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- JUJBNYBVVQSIOU-UHFFFAOYSA-M sodium;4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate Chemical compound [Na+].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C(=CC(=CC=2)S([O-])(=O)=O)S([O-])(=O)=O)=N1 JUJBNYBVVQSIOU-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Emergency Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cosmetics (AREA)
Abstract
Description
本発明は、エゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法とエゴマ油ビューティーオイル及び植物性オメガ−３を有効成分として含有する化粧料組成物及びこれを含む化粧品に関するものであり、より詳細には、エゴマを冷圧着で搾油し、搾油したエゴマ油を冷低温で凝固及び沈殿させ、冷低温から生成された凝固浮遊物を除去し、同時に植物性オメガ−３であるリノレン酸の含有を向上させることができるエゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法とエゴマ油ビューティーオイル及び植物性オメガ−３を有効成分として含有する化粧料組成物及びこれを含む化粧品に関するものである。 The present invention is a cosmetic composition containing egoma oil beauty oil and vegetable omega-3 capable of blocking the ultraviolet rays of egoma oil, antioxidative effect, moisturizing and whitening the skin, and preventing wrinkles, stains and freckles. The present invention relates to a cosmetic composition containing beauty oil and vegetable omega-3 as active ingredients, and cosmetics containing the same. More specifically, the sesame oil is squeezed by cold pressure bonding and squeezed. UV blocking effect and antioxidant of egoma oil that can coagulate and precipitate oil at cold and low temperature, remove coagulated suspended matter generated from cold and low temperature, and at the same time improve the content of linolenic acid, which is a vegetable omega-3. Efficacy, skin moisturizing and whitening, and method for producing cosmetic composition containing egoma oil beauty oil and vegetable omega-3 capable of preventing wrinkles, stains and freckles, and egoma oil beauty oil and vegetable omega- It relates to a cosmetic composition containing 3 as an active ingredient and cosmetics containing the same.
大韓民国の代表の油脂作物の一つであるエゴマは、シソ科に属する一年生の植物であって、大韓民国を含む東南アジアが原産であり、昔から単純な食用だけでなく、健康食品、高血圧の予防などの食品として多様に利用してきた。 Perilla frutescens, one of the representative oil and fat crops of the Republic of Korea, is an annual plant belonging to the Labiatae family and is native to Southeast Asia including the Republic of Korea. It has long been not only a simple food, but also a health food and prevention of high blood pressure. It has been used in various ways as a food product.
大韓民国でのエゴマの利用は、統一新羅時代にゴマと一緒に栽培した記録があり、全国の麓や道端の湿地で簡単に育ち、全国どこでも分布している。 The use of perilla in the Republic of Korea has a record of being cultivated with sesame during the Unified Silla era, and it grows easily at the foot of the country and in wetlands on the roadside, and is distributed everywhere in the country.
エゴマは、脂肪４５％、タンパク質１８％、炭水化物２８％の３大栄養素を等しく持っており、重要な油脂源として古くから使用されてきた。特に、エゴマから抽出したエゴマ油には、脂肪酸が約３５〜４６％であり、飽和脂肪酸が約１０％前後であり、約９０％前後の不飽和脂肪酸を含有しており、そのうちの５０％以上が、ｎ−３系多価不飽和脂肪酸系であり、動脈硬化、血清脂質の変化、高血圧、血圧の低下、および血栓症の改善、癌細胞の増殖抑制、学習能力の向上、認知症とうつ病、網膜と脳の発達、アレルギー体質の改善、寿命の延長などエゴマ油が成人病の予防に効果的な生理作用を有しており、皮膚のシミ、そばかす抑制と保湿、美白などアンチエイジングの効果があるので、その利用価値が非常に高まっている。 Perilla has three major nutrients, 45% fat, 18% protein and 28% carbohydrate, and has long been used as an important source of fats and oils. In particular, the perilla oil extracted from perilla contains about 35 to 46% fatty acids, about 10% saturated fatty acids, and about 90% unsaturated fatty acids, of which 50% or more. However, it is an n-3 polyunsaturated fatty acid system, and has arteriosclerosis, changes in serum lipids, hypertension, decreased blood pressure, and improvement of thrombosis, suppression of cancer cell growth, improvement of learning ability, dementia and depression. Perilla oil has effective physiological effects on the prevention of adult diseases such as diseases, development of retina and brain, improvement of allergic constitution, extension of lifespan, and anti-aging such as skin stains, freckle suppression and moisturization, and whitening. Since it is effective, its utility value is greatly increased.
エゴマの効能は昔から東医宝鑑によれば、エゴマは性質が暖かく、毒がなく、味は酸っぱく、気を落ち着かせてくれる。 According to Dongui Bogam, the efficacy of perilla is warm, non-poisonous, sour in taste, and calming.
また、肝を豊かにし、その種は粥を沸かして食べると、私たちの体を滑らかに美しく作ってくれて元気を回復させてくれる。「方薬合編」には、精髄を保護してくれて、渇きと咳嗽をなくし、体内の毒素を除去し、血液をきれいにしてくれる効能があり、他の多くの文献によれば、エゴマはリノレン酸とビタミンＥ、Ｆがたくさん入っているので、女性の健康と美肌に絶対に必要なものであり、肌が粗くそばかすやシミが多い人と、日焼け後なかなか回復がない人に良いと紹介されている。 It also enriches the liver, and when the seeds are boiled and eaten, it makes our body smooth and beautiful and restores energy. "Pharmaceutical combination" has the effect of protecting the essence, eliminating thirst and coughing, removing toxins in the body, and cleaning the blood. According to many other documents, freckles Since it contains a lot of linolenic acid and vitamins E and F, it is absolutely necessary for women's health and beautiful skin, and it is good for people with rough skin and many freckles and age spots, and people who do not recover easily after sunburn. Introduced.
エゴマからエゴマ油を抽出する方法には、エゴマを炒めて圧着する炒め加熱圧着法が大部分を占めており、いくつかの高価な機械を利用して、溶媒によって抽出する超臨界抽出法があるが、溶媒の危険性に消費者に多くの拒否感が付いている。 Most of the methods for extracting perilla oil from perilla are the stir-fry heat crimping method in which perilla is roasted and crimped, and there is a supercritical extraction method in which the perilla is extracted with a solvent using some expensive machines. However, the danger of solvents is often rejected by consumers.
また、エゴマを炒めず、搾油する冷圧着方式（Ｃｏｏｌ Ｐｒｅｓｓｅｄｅ）にエゴマを乾燥した後、そのまま搾油して沈殿、濾過段階を通じてエゴマ油を製造する方法がある。 In addition, there is a method of producing perilla oil through a cold pressure bonding method (Cool Pressed) in which the perilla is not fried and oil is squeezed, and then the perilla is squeezed as it is, precipitated, and filtered.
前記炒め加熱圧着法は、高温（１６０〜２３０℃）にエゴマを炒めてエゴマ油を製造する方法として、ベンゾピレンなどの物質が検出される問題があり、前記超臨界抽出法は、迅速な抽出と選択抽出が可能で、溶媒抽出法に比べて有害性溶媒の残存リスクがなく、天然物または食品などの熱に敏感な物質を抽出することができるが、生産コストが非常に高く、現実的ではない。 The stir-fry heat-bonding method has a problem that a substance such as benzopyrene is detected as a method for producing stir-fry oil by frying egoma at a high temperature (160 to 230 ° C.), and the supercritical extraction method is a method of rapid extraction. Selective extraction is possible, there is no residual risk of harmful solvents compared to the solvent extraction method, and heat-sensitive substances such as natural products or foods can be extracted, but the production cost is very high and it is not realistic. Absent.
エゴマ油は酸化されやすい植物油で流通期間が短く、開封後はなるべく迅速に消費することを推奨しており、保管の際には、低温で冷蔵保管することを推奨している。 Perilla oil is a vegetable oil that is easily oxidized and has a short distribution period. It is recommended that it be consumed as soon as possible after opening, and it is recommended that it be stored refrigerated at a low temperature.
しかしながら、エゴマ油には、さまざまな脂肪酸が含有されており、冷低温または冷蔵保管時、エゴマ油が凝固されて白い斑点や白い沈殿物が発生する。 However, perilla oil contains various fatty acids, and when stored at low temperature or refrigerated, the perilla oil is coagulated to generate white spots and white precipitates.
よって、国内外の流通しているエゴマ油には、「冬季または低い温度や冷蔵保管時に凝固凍結現象（白い斑点や沈殿物の発生）が生じるが、製品には異常がありません。」という注意事項が記録された製品を簡単に見つけることができる。これは、前述したエゴマ油の凝固現象から発生する問題について、消費者にとって否定的な認識を持たないようにするためのもので、エゴマ油から発生する凝固凍結の現象に起因する浮遊物の発生に対する根本的な問題を解決していない。 Therefore, it should be noted that the perilla oil distributed in Japan and overseas has a coagulation-freezing phenomenon (white spots and precipitates) in winter or during low temperature or refrigerated storage, but there is no abnormality in the product. You can easily find the product with the recorded product. This is to prevent consumers from having a negative perception of the problem caused by the above-mentioned coagulation phenomenon of perilla oil, and the generation of suspended matter caused by the coagulation freezing phenomenon generated from perilla oil. Has not solved the underlying problem with.
即ち、エゴマ油には一般的に植物性オメガ３脂肪酸と言うα−ｌｉｎｏｌｅｎｉｃ ａｃｉｄが主成分として、約６０％ほどが含有されているが、これは酸敗しやすい成分として、冷蔵保管が不可欠であり、高温で加熱処理して搾油する場合には、ベンゾピレンなどの発癌性物質及びトランス脂肪酸が生成されるので、エゴマ油を搾油するための加工過程だけではなく、保管の過程でもエゴマ油の特性に合わせて加工や搾油、保管まで行われるが、このような過程が順調に行われていないため、優れた植物油であるエゴマ油を適切にビューティー化粧品の原料として使用していない。また、酸敗を防ぐために、冷蔵保管を行う場合には、低温により生成される浮遊物のために、これを摂取する人々に不快感を与えることになる。 That is, perilla oil generally contains about 60% of α-linolenic acid, which is a vegetable omega-3 fatty acid, as a main component, but it is indispensable to store it in a refrigerator as a component that easily loses acid. When oil is squeezed by heat treatment at high temperature, carcinogens such as benzopyrene and trans fatty acids are produced. Therefore, not only in the processing process for squeezing perilla oil, but also in the storage process, the characteristics of perilla oil are affected. In addition, processing, oil extraction, and storage are performed, but since such a process is not carried out smoothly, perilla oil, which is an excellent vegetable oil, is not properly used as a raw material for beauty cosmetics. In addition, when refrigerated storage is carried out to prevent rancidification, the suspended matter produced by low temperature causes discomfort to people who ingest it.
したがって、本発明では、エゴマを冷圧着で搾油し、搾油したエゴマ油を冷低温で凝固及び沈殿させ、冷低温から生成された凝固浮遊物を除去し、同時に植物性オメガ−３であるリノレン酸の含有を向上させることができるエゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法とエゴマ油ビューティーオイル及び植物性オメガ−３を有効成分として含有する化粧料組成物及びこれを含む化粧品を提供することになる。 Therefore, in the present invention, perilla is squeezed by cold pressure bonding, the squeezed perilla oil is coagulated and precipitated at low and low temperatures, and coagulated suspended matter generated from cold and low temperatures is removed, and at the same time, linolenic acid, which is a vegetable omega-3. Contains perilla oil beauty oil and vegetable omega-3, which can improve the content of perilla oil, which can improve the UV blocking effect, antioxidant effect, moisturize and whiten the skin, and prevent wrinkles, stains and freckles. A method for producing a cosmetic composition, a cosmetic composition containing perilla oil beauty oil and vegetable omega-3 as active ingredients, and a cosmetic containing the same will be provided.
したがって、本発明は、前記のような問題点を解決するために提案された技術として、発明の目的は、エゴマを冷圧着で搾油し、搾油したエゴマ油を冷低温で凝固及び沈殿させ、冷低温から生成された凝固浮遊物を除去し、同時に、人体に有害なトランス脂肪酸とベンゾピレンなどの発癌物質が生成されず、植物性オメガ−３であるリノレン酸の含有を向上させることができる化粧料組成物の製造方法の提供にある。 Therefore, the present invention is a technique proposed to solve the above-mentioned problems, and an object of the present invention is to squeeze perilla by cold pressure bonding, coagulate and precipitate the squeezed perilla oil at low temperature, and cool it. A cosmetic that can remove coagulated suspended matter generated from low temperature, and at the same time, it does not produce carcinogens such as trans fatty acids and benzopyrene that are harmful to the human body, and can improve the content of linolenic acid, which is a vegetable omega-3. A method for producing a composition is provided.
本発明の他の目的は、人体の皮膚に有益ながらも、紫外線遮断効果と抗酸化効能と皮膚の保湿、美白としわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイルと植物性オメガ−３を有効成分として含有する化粧料組成物及びこれを含む化粧品の提供にある。 Another object of the present invention is egoma oil beauty oil and vegetable omega-3, which are beneficial to the human skin but capable of blocking ultraviolet rays, antioxidative effect, moisturizing the skin, whitening and preventing wrinkles, stains and freckles. The present invention is to provide a cosmetic composition containing the above as an active ingredient and cosmetics containing the same.
本発明のもう一つの目的は、エゴマ油の東医宝鑑効能を科学的に立証されたデータの提供にある。 Another object of the present invention is to provide data that scientifically proves the efficacy of Dongui Bogam of perilla oil.
本発明が解決しようとする課題を達成するために、本発明の一実施例に係るエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法は、エゴマの洗浄段階Ｓ１００を介して洗浄されたエゴマを水分率が１〜１５％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させる乾燥段階Ｓ２００と；前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜６０℃の温度で圧力を加えて、１〜６０分間エゴマ油を搾油する搾油段階Ｓ３００と；前記搾油段階Ｓ３００を介して搾油されたエゴマ油が２５℃以下になるように冷低温処理する冷低温処理段階Ｓ５００と；前記低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜５０μｍの食品用濾過網を利用して濾過させる濾過段階Ｓ６００と；を含んで構成されることを特徴とする。 In order to achieve the problem to be solved by the present invention, the method for producing a cosmetic composition containing perilla oil beauty oil and vegetable omega-3 according to an embodiment of the present invention is a perilla cleaning step S100. In the drying step S200, in which the perilla washed through the oil is dried with cold and warm air at 15 to 80 ° C. for 1 to 5 hours so that the moisture content becomes 1 to 15%; the perilla dried through the drying step S200. In the oil squeezing device, pressure is applied at a temperature of 0 to 60 ° C. to squeeze the perilla oil for 1 to 60 minutes; the perilla oil squeezed through the oil squeezing step S300 is reduced to 25 ° C. or lower. A cold / low temperature treatment step S500 for cold / low temperature treatment and a filtration step S600 for filtering perilla oil cold / low temperature treated through the low temperature treatment step S500 using a 1 to 50 μm food filter net. It is characterized by including.
したがって、以上の構成及び作用を有する本発明によるエゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法とエゴマ油ビューティーオイルと植物性オメガ−３を有効成分として含有する化粧料組成物及びこれを含む化粧品は、エゴマを冷圧着で搾油し、搾油したエゴマ油を冷低温で凝固及び沈殿させ、冷低温から生成された凝固浮遊物を除去し、同時に、人体に有害なトランス脂肪酸とベンゾピレンなどの発癌物質が生成されず、植物性オメガ−３であるリノレン酸の含有を向上させることができるようすることによって、冷低温で凝固浮遊物が生成されない化粧品用エゴマ油を提供することになる。 Therefore, perilla oil beauty oil and vegetable omega-3, which have the above-mentioned composition and action, can block the ultraviolet rays of perilla oil, have an antioxidant effect, moisturize and whiten the skin, and prevent wrinkles, stains and freckles. Perilla oil A method for producing a cosmetic composition containing perilla, a perilla composition containing perilla oil and vegetable omega-3 as active ingredients, and cosmetics containing the perilla oil are squeezed perilla by cold pressure bonding. The oil is coagulated and precipitated at cold and low temperatures to remove coagulated suspended matter generated from cold and low temperatures, and at the same time, trans fatty acids and carcinogens such as benzopyrene, which are harmful to the human body, are not produced, and linolene, which is a plant omega-3. By making it possible to improve the content of the acid, it is possible to provide the perilla oil for cosmetics, which does not generate coagulated suspended matter at low temperature.
また、人体の皮膚に有益ながらも、紫外線遮断効果と抗酸化効能と皮膚の保湿、美白としわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイルと植物性オメガ−３を有効成分として含有する化粧料組成物及びこれを含む化粧品を提供することになる。 In addition, it contains egoma oil beauty oil and vegetable omega-3, which are beneficial to the human skin but have UV blocking effect, antioxidant effect, skin moisturizing effect, whitening and wrinkle, spots and freckles prevention. A cosmetic composition and a cosmetic containing the same will be provided.
また、エゴマ油の東医宝鑑効能を科学的に立証するデータを提供する効果がある。 It also has the effect of providing data that scientifically proves the efficacy of Dongui Bogam of perilla oil.
以下、本願の発明が属する技術分野で通常の知識を有する者が容易に実施できるように、本発明の実施の形態を挙げ詳細に説明する。本発明の実施の形態は、当業界で平均的な知識を有する者に本発明をさらに完全に説明するために提供されるものである。したがって、本発明の実施の形態は、いくつかの他の形態に変形することができ、本発明の範囲が以下の説明する実施例に限定されるものではない。 Hereinafter, embodiments of the present invention will be described in detail so that a person having ordinary knowledge in the technical field to which the invention of the present application belongs can easily carry out the invention. Embodiments of the present invention are provided to further fully illustrate the invention to those with average knowledge in the art. Therefore, the embodiments of the present invention can be transformed into some other embodiments, and the scope of the present invention is not limited to the examples described below.
本発明の明細書全体では、どの部分がどのような構成要素を “含む”とする時、これは特に反対される記載がない限り、他の構成要素を除外するのではなく、他の構成要素をさらに含むことができることを意味する。 In the entire specification of the present invention, when which part “contains” what component, this does not exclude other components unless otherwise stated, but other components. Means that can be further included.
本発明の明細書全体では、いくつかの段階が他の段階と“上に”または“前に”位置するとする時、これはどの段階が他の段階と直接時系列的な関係にある場合だけでなく、各段階後の混合する段階のように、２つの段階の順序に系列的な順序が変わることができる間接的、時系列的な関係にある場合と同じ権利を含むことができる。 In the entire specification of the present invention, when some stages are "above" or "previous" to other stages, this is only if which stage has a direct chronological relationship with the other stages. Rather, it can include the same rights as in an indirect, chronological relationship, where the sequence order can be changed to the sequence of the two stages, such as the mixed stages after each stage.
本発明の明細書全体で使用される程度の用語“約”、“実質的に”などは、言及された意味に固有の製造および物質の許容誤差が提示されるとき、その数値でまたはその数値に近い意味で使用され、本発明の理解を助けるために正確または絶対的な数値が記載されて開示内容を非良心的な侵害者が不当に利用することを防止するために使用される。 The terms "about", "substantially", etc., as used throughout the specification of the present invention, are used numerically or numerically when the manufacturing and material tolerances inherent in the referred meaning are presented. It is used in a sense close to, and is used to prevent an unscrupulous infringer from unfairly using the disclosed content with accurate or absolute numerical values to aid the understanding of the present invention.
本願明細書全体で使用される用語である“〜する段階”または“〜の段階”は“〜のための段階”を意味しない。 The terms "step to" or "step to" as used throughout the specification do not mean "step to".
図１は、本発明の第１の実施例によるエゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法を示すフローチャートであり、図２は、本発明の第２の実施例ないし第３の実施例に係るエゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法を示すフローチャートである。 FIG. 1 shows the UV blocking effect of the egoma oil according to the first embodiment of the present invention, the antioxidant effect, the moisturizing and whitening of the skin, and the egoma oil beauty oil and the vegetable omega capable of preventing wrinkles, stains and freckles. 3 is a flowchart showing a method for producing a cosmetic composition containing 3; FIG. 2 shows the ultraviolet blocking effect, antioxidant effect, and skin of the egoma oil according to the second to third embodiments of the present invention. It is a flowchart which shows the manufacturing method of the cosmetic composition containing the egoma oil beauty oil and vegetable omega-3 which can moisturize and whiten, and prevent wrinkles, stains and freckles.
本発明は、エゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法に関するものである。 The present invention is a cosmetic composition containing perilla oil beauty oil and vegetable omega-3 capable of blocking ultraviolet rays of perilla oil, antioxidative effect, moisturizing and whitening skin, and preventing wrinkles, stains and freckles. It is related to the manufacturing method of.
前述した凝固浮遊物とは、エゴマ油を低温で保管したり、エゴマ油を冷蔵保管したとき、室温よりも低い温度で発生する可能性のある凝固浮遊物、結露浮遊物、凍結浮遊物の両方を含むものと理解するのが望ましい。 The above-mentioned solidified suspended matter is both solidified suspended matter, condensed suspended matter, and frozen suspended matter that may be generated at a temperature lower than room temperature when the perilla oil is stored at a low temperature or the perilla oil is stored in a refrigerator. It is desirable to understand that it includes.
以下本発明を、図面を参照して説明すれば、第１の実施例に係る本発明は、図１に示すように、乾燥段階Ｓ２００、搾油段階Ｓ３００、冷低温処理段階Ｓ５００、及び濾過段階Ｓ６００を含んで構成されている。 Hereinafter, the present invention will be described with reference to the drawings. As shown in FIG. 1, the present invention according to the first embodiment has a drying step S200, an oil extraction step S300, a cold / low temperature treatment step S500, and a filtration step S600. Is configured to include.
さらに詳細には、エゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法において、洗浄されたエゴマを水分率が１〜１５％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させる乾燥段階Ｓ２００と；前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜６０℃の温度で圧力を加えて、１〜６０分間エゴマ油を搾油する搾油段階Ｓ３００と；前記搾油段階Ｓ３００を介して搾油されたエゴマ油が２５℃以下になるように冷低温処理する冷低温処理段階Ｓ５００と；前記低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜５０μｍの食品用濾過網を利用して濾過させる濾過段階Ｓ６００と；を含んで構成されている。 More specifically, in the method for producing a cosmetic composition containing perilla oil beauty oil and vegetable omega-3, the washed perilla is dried at 15 to 80 ° C. so that the water content is 1 to 15%. In the drying step S200, which is dried with cold and warm air for 1 to 5 hours; the perilla oil dried through the drying step S200 is put into an oil squeezer, and pressure is applied at a temperature of 0 to 60 ° C., and perilla oil is applied for 1 to 60 minutes. The oil squeezing step S300; the sesame oil squeezed through the oil squeezing step S300 is subjected to cold / low temperature treatment so that the temperature of the perilla oil is 25 ° C. or lower; the cold / low temperature treatment step S500; It is composed of a filtration step S600 in which the perilla oil is filtered using a 1 to 50 μm food filter net;
具体的に説明すれば、前記乾燥段階Ｓ２００は、洗浄されたエゴマを水分率が１〜１５％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させるように構成されている。 Specifically, the drying step S200 is configured to dry the washed perilla with cold and warm air at 15 to 80 ° C. for 1 to 5 hours so that the moisture content is 1 to 15%. ..
前記乾燥段階Ｓ２００は、洗浄されたエゴマの水分率を下げることにより、抽出収率を高めるだけでなく、エゴマを冷温風で炒めることにより、搾油されたエゴマ油の香ばしい香りと風味を生かすことができるようになる。 In the drying step S200, not only the extraction yield is increased by lowering the water content of the washed perilla, but also the fragrant aroma and flavor of the squeezed perilla oil can be utilized by frying the perilla with cold and warm air. become able to.
前記乾燥段階Ｓ２００は、水分率が１〜１５％になるようにすることによって、エゴマの表皮とエゴマの中身との間の水分を乾燥し、エゴマの表皮との間の水分を乾燥させて、エゴマの粒子が等しく配列されるようにすることによって、搾油時、エゴマ粒子が圧力を均一に受けるようにして抽出収率を高めることができるように構成される。 In the drying step S200, the water content between the perilla skin and the contents of the perilla is dried by adjusting the water content to 1 to 15%, and the water between the perilla skin and the perilla skin is dried. By making the perilla particles evenly arranged, the perilla particles are uniformly subjected to pressure during oil extraction, and the extraction yield can be increased.
前記乾燥段階Ｓ２００は、１５〜８０℃の冷温風で１〜５時間乾燥されることによって、強力な熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されている。 The drying step S200 is such that by drying with cold and warm air at 15 to 80 ° C. for 1 to 5 hours, strong heat prevents the production of carcinogens such as trans fatty acids, saturated fats, and benzopyrene. It is configured.
前記乾燥条件を外れた場合には、強い熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどが上昇するので、前述した閾値条件内で乾燥を行うのが望ましい。 When the drying conditions are not met, trans fatty acids, saturated fats, benzopyrene and the like increase due to strong heat, so it is desirable to perform drying within the above-mentioned threshold conditions.
一方、付加的な面に沿って、前記乾燥段階Ｓ２００は、エゴマが等しく混合されるように攪拌する攪拌段階と一緒に遂行されるように構成されることができる。 On the other hand, along the additional surface, the drying step S200 can be configured to be carried out in conjunction with a stirring step of stirring the perilla so that it is equally mixed.
前記搾油段階Ｓ３００は、前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜６０℃の温度で圧力を加えて、１〜６０分間エゴマ油を搾油するように構成されている。 The oil extraction step S300 is configured to put the perilla dried through the drying step S200 into an oil squeezer and apply pressure at a temperature of 0 to 60 ° C. to squeeze the perilla oil for 1 to 60 minutes. There is.
前記搾油段階Ｓ３００は、４００〜９００ｋｇｆ／ｃｍ２の圧力で搾油することができる。 The oil extraction step S300 can be extracted at a pressure of 400 to 900 kgf / cm 2 .
前記搾油段階Ｓ３００は、作業場の温度が０〜６０℃の範囲内になるように温度を設定した後に、遂行されることによって、強力な熱によりトランス脂肪酸と飽和脂肪及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されている。 The oil extraction step S300 is carried out after setting the temperature so that the temperature of the workplace is in the range of 0 to 60 ° C., and by carrying out the process, trans fatty acids and carcinogens such as saturated fat and benzopyrene are produced by strong heat. It is configured to prevent this from happening.
好ましくは、前記作業場の温度は２５℃に設定することになる。これを超過する場合には、強い熱により発癌物質が多量に含まれており、未満の場合には、搾油が正しく遂行されないため、前述した範囲内で、特に最適の温度条件を希望する場合には、２５℃に設定して搾油プロセスを遂行することになる。 Preferably, the temperature of the workplace will be set to 25 ° C. If it exceeds this, a large amount of carcinogen is contained due to strong heat, and if it is less than this, oil extraction will not be performed correctly. Therefore, if the optimum temperature conditions are desired within the above range. Will carry out the oil extraction process at 25 ° C.
また、前記搾油段階Ｓ３００は、エゴマの量に応じて搾油時間を適切に調節するように構成されるのが望ましい。 Further, it is desirable that the oil extraction step S300 is configured so as to appropriately adjust the oil extraction time according to the amount of perilla.
前記搾油段階Ｓ３００は、例えば、２００ｋｇｆ／ｃｍ２まで搾油圧力を継続的に上昇させた後、２００ｋｇｆ／ｃｍ２の圧力に到達すれば、５分間搾油圧力を１０〜５０ｋｇｆ／ｃｍ２の範囲内で段階的に上昇させることによって、時間に応じて段階的に搾油圧力を上昇させるように構成されるのが望ましい。 The oil extraction step S300, for example, after continuously increasing the oil extraction pressure to 200 kgf / cm 2, when reaching a pressure of 200 kgf / cm 2, for 5 minutes expeller pressure in the range of 10~50kgf / cm 2 It is desirable that the oil extraction pressure is gradually increased according to the time by gradually increasing the oil extraction pressure.
これは、搾油圧力に応じてエゴマに発生する可能性のある摩擦熱を最小限に抑えることによって、強い熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されるのが望ましい。 This is to prevent strong heat from producing carcinogens such as trans fatty acids, saturated fats, and benzopyrene by minimizing the frictional heat that can be generated in the perilla in response to oil extraction pressure. It is desirable to be configured in.
また、前記冷低温処理段階Ｓ５００は、前記搾油段階Ｓ３００を介して搾油されたエゴマ油が２５℃以下になるように冷低温処理するものである。 Further, the cold / low temperature treatment step S500 is a cold / low temperature treatment so that the perilla oil squeezed through the oil extraction step S300 is 25 ° C. or lower.
前記冷低温処理段階Ｓ５００は、搾油されたエゴマ油を冷低温処理することにより、エゴマ油に含まれる様々な成分が、それぞれの融点以下に到達するようにすることによって、人為的にエゴマ油に含まれている様々な成分の凝固浮遊物が生成されるように構成される。 In the cold / low temperature treatment step S500, the squeezed perilla oil is artificially made into perilla oil by cold / low temperature treatment so that various components contained in the perilla oil reach below their respective melting points. It is configured to produce coagulated suspended matter of various components contained.
即ち、凝固浮遊物を除去する過程である。 That is, it is a process of removing solidified suspended matter.
そして、エゴマ油に含有された植物性オメガ−３であるリノレン酸の含有を向上させることになる。 Then, the content of linolenic acid, which is a vegetable omega-3 contained in perilla oil, will be improved.
このとき、最適の温度条件は、搾油されたエゴマ油が５℃以下になるように冷低温処理するものである。 At this time, the optimum temperature condition is that the squeezed perilla oil is subjected to cold and low temperature treatment so as to be 5 ° C. or lower.
そして、前記冷低温処理段階Ｓ５００は、エゴマ油の温度が常温よりも低い温度になるようにするもので、エゴマ油を冷低温加工して、エゴマ油の温度が常温よりも低い温度になるようにする冷低温処理、エゴマ油を冷蔵加工してエゴマ油の温度を下げる冷蔵処理、エゴマ油を冷凍加工してエゴマ油を凍結させる凍結処理のうち少なくともいずれか一つまたは複数の加工処理方法で冷低温処理するのが望ましい。 Then, in the cold / low temperature treatment step S500, the temperature of the perilla oil is set to be lower than the normal temperature, and the perilla oil is cold-low processed so that the temperature of the perilla oil is lower than the normal temperature. At least one or more of the cold and low temperature treatment, the cold treatment to lower the temperature of the perilla oil by refrigerating the perilla oil, and the freezing treatment to freeze the perilla oil and freeze the perilla oil. Cold and low temperature treatment is desirable.
このとき、他の付加的な面に沿って、前記冷低温処理段階Ｓ５００の後に、冷低温処理されたエゴマ油を沈殿タンクに投入し、５分以上、自然沈殿させる沈殿段階Ｓ４００を再遂行するように構成することができる。 At this time, along with other additional surfaces, after the cold / low temperature treatment step S500, the cold / low temperature treated perilla oil is put into the settling tank, and the settling step S400 for spontaneous precipitation is performed again for 5 minutes or more. It can be configured as follows.
また、前記濾過段階Ｓ６００は、前記低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜５０μｍの食品用濾過網を利用して濾過させるように構成されている。 Further, the filtration step S600 is configured to filter perilla oil that has been subjected to cold / low temperature treatment through the low temperature treatment step S500 using a 1 to 50 μm food filtration net.
前記濾過段階Ｓ６００は、前記冷低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油から生成された凝固浮遊物を濾過して、今後、冷低温でもエゴマ油が凝固浮遊物なしでクリアな状態を維持できるように構成されている。 The filtration step S600 filters the coagulated suspended matter produced from the perilla oil that has been subjected to the cold / low temperature treatment through the cold / low temperature treatment step S500, and the perilla oil is in a state of being clear without the coagulated suspended matter even at a cold / low temperature in the future. It is configured to be able to maintain.
前記濾過段階Ｓ６００は、作業場の温度が２５℃以下になるように温度を設定した後に遂行されることによって、エゴマ油に含まれる様々な成分のそれぞれの融点以下である状態で濾過して、エゴマ油に含まれるさまざまな成分から生成された凝固浮遊物が融点に達していない状態で、すべて濾過されるように構成されるのが望ましい。 The filtration step S600 is carried out after setting the temperature so that the temperature of the workplace is 25 ° C. or lower, so that the perilla is filtered in a state of being equal to or lower than the melting point of each of the various components contained in the perilla oil. It is desirable that all the solidified suspensions produced from the various components contained in the oil have not reached the melting point and are all filtered.
このとき、冷低温で凝固されたエゴマ油を常温や冷低温処理の段階で高い温度で濾過する場合に凝固が再び溶けて元の状態になるものである。 At this time, when the perilla oil coagulated at cold and low temperatures is filtered at a high temperature at the stage of normal temperature or cold and low temperature treatment, the coagulation melts again and returns to the original state.
一方、第２の実施例に係る本発明は、図２に示すように、エゴマの洗浄段階Ｓ１００、乾燥段階Ｓ２００、搾油段階Ｓ３００、沈殿段階Ｓ４００、冷低温処理段階 Ｓ５００、及び濾過段階Ｓ６００を含んで構成されている。 On the other hand, as shown in FIG. 2, the present invention according to the second embodiment includes a perilla washing step S100, a drying step S200, an oil extraction step S300, a precipitation step S400, a cold / low temperature treatment step S500, and a filtration step S600. It is composed of.
さらに詳細には、エゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法において、エゴマの洗浄器に投入して１〜３０分間洗浄するエゴマの洗浄段階Ｓ１００と；前記エゴマの洗浄段階Ｓ１００を介して洗浄されたエゴマを水分率が１〜１５％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させる乾燥段階Ｓ２００と；前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜６０℃の温度で圧力を加えて、１〜６０分間エゴマ油を搾油する搾油段階Ｓ３００と；前記搾油段階Ｓ３００を介して搾油されたエゴマ油を沈殿タンクに投入し、１分以上、自然沈殿させる沈殿段階Ｓ４００と；前記沈殿段階Ｓ４００を介して沈殿させたエゴマ油が２５℃以下になるように冷低温処理する冷低温処理段階Ｓ５００と；前記低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜５０μｍの食品用濾過網を利用して濾過させる濾過段階Ｓ６００と；を含んで構成されている。 More specifically, a cosmetic composition containing perilla oil beauty oil and vegetable omega-3 capable of blocking the ultraviolet rays of perilla oil, antioxidative effect, moisturizing and whitening the skin, and preventing wrinkles, stains and freckles. In the method for producing a product, the perilla is put into a perilla washer and washed for 1 to 30 minutes with the perilla cleaning step S100; the perilla washed through the perilla cleaning step S100 has a water content of 1 to 15%. In the drying step S200, which is dried with cold and warm air at 15 to 80 ° C. for 1 to 5 hours; the perilla dried through the drying step S200 is put into an oil squeezer, and pressure is applied at a temperature of 0 to 60 ° C. The perilla oil is squeezed for 1 to 60 minutes in the oil squeezing step S300; the perilla oil squeezed through the squeezing step S300 is put into a settling tank and naturally settled for 1 minute or more. In the cold / low temperature treatment step S500 in which the perilla oil precipitated via S400 is cold / low temperature treated so as to be 25 ° C. or lower; the perilla oil cold / low temperature treated through the low temperature treatment step S500 is used for food of 1 to 50 μm. It is configured to include a filtration step S600 and; which are filtered using a filtration net.
具体的に説明すれば、前記エゴマの洗浄段階Ｓ１００は、エゴマを洗浄器に投入して１〜３０分間洗浄するように構成されている。 Specifically, the cleaning step S100 of the perilla is configured to put the perilla into a washing device and wash it for 1 to 30 minutes.
前記乾燥段階Ｓ２００は、洗浄されたエゴマを水分率が１〜１５％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させるように構成されている。 The drying step S200 is configured to dry the washed perilla with cold / warm air at 15 to 80 ° C. for 1 to 5 hours so that the moisture content is 1 to 15%.
前記乾燥段階Ｓ２００は、洗浄されたエゴマの水分率を下げることにより、抽出収率を高めるだけでなく、エゴマを熱風で炒めることにより、搾油されたエゴマ油の香ばしい香りと風味を生かすことができるよう構成される。 In the drying step S200, not only the extraction yield is increased by lowering the water content of the washed perilla, but also the fragrant aroma and flavor of the squeezed perilla oil can be utilized by frying the perilla with hot air. Is configured.
前記乾燥段階Ｓ２００は、水分率が１〜１５％になるようにすることによって、エゴマの表皮とエゴマの中身との間の水分を乾燥し、エゴマの表皮との間の水分を乾燥させて、エゴマの粒子が等しく配列されるようにすることによって、搾油時、エゴマ粒子が圧力を均一に受けるようにして抽出収率を高めることができるよう構成されている。 In the drying step S200, the water content between the perilla skin and the contents of the perilla is dried by adjusting the water content to 1 to 15%, and the water between the perilla skin and the perilla skin is dried. By making the perilla particles evenly arranged, the perilla particles are uniformly pressured during oil extraction, and the extraction yield can be increased.
前記乾燥段階Ｓ２００は、１５〜８０℃の冷温風で１〜５時間乾燥されることによって、強力な熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されている。 The drying step S200 is such that by drying with cold and warm air at 15 to 80 ° C. for 1 to 5 hours, strong heat prevents the production of carcinogens such as trans fatty acids, saturated fats, and benzopyrene. It is configured.
この時、前記乾燥段階Ｓ２００は、エゴマが等しく混合されるように攪拌する攪拌段階と一緒に遂行されるように構成されることができる。 At this time, the drying step S200 can be configured to be carried out together with a stirring step of stirring so that the perilla is equally mixed.
また、前記搾油段階Ｓ３００は、前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜６０℃の温度で圧力を加えて、１〜６０分間エゴマ油を搾油するように構成されている。 Further, the oil extraction step S300 is configured such that the perilla oil dried through the drying step S200 is put into an oil press and pressure is applied at a temperature of 0 to 60 ° C. to squeeze the perilla oil for 1 to 60 minutes. Has been done.
前記搾油段階Ｓ３００は、４００〜９００ｋｇｆ／ｃｍ２の圧力で搾油することができる。前記搾油段階Ｓ３００は、作業場の温度が０〜６０℃の範囲内になるように温度を設定した後に遂行されることによって、強力な熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されている。 The oil extraction step S300 can be extracted at a pressure of 400 to 900 kgf / cm 2 . The oil extraction step S300 is carried out after setting the temperature so that the temperature of the workplace is in the range of 0 to 60 ° C., so that strong heat produces carcinogens such as trans fatty acids, saturated fats, and benzopyrene. It is configured to prevent this from happening.
前記搾油段階Ｓ３００は、エゴマの量に応じて搾油時間を適切に調節するように構成されるのが望ましい。 It is desirable that the oil extraction step S300 is configured so that the oil extraction time is appropriately adjusted according to the amount of perilla.
前記搾油段階Ｓ３００は、例えば、２００ｋｇｆ／ｃｍ２まで搾油圧力を継続的に上昇させた後、２００ｋｇｆ／ｃｍ２の圧力に到達すれば、５分間搾油圧力を１０〜５０ｋｇｆ／ｃｍ２の範囲内で段階的に上昇させることによって、時間に応じて段階的に搾油圧力を上昇させるように構成されるのが望ましい。 The oil extraction step S300, for example, after continuously increasing the oil extraction pressure to 200 kgf / cm 2, when reaching a pressure of 200 kgf / cm 2, for 5 minutes expeller pressure in the range of 10~50kgf / cm 2 It is desirable that the oil extraction pressure is gradually increased according to the time by gradually increasing the oil extraction pressure.
これは、搾油圧力に応じてエゴマから発生する可能性のある摩擦熱を最小限に抑えることによって、強い熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されているものと理解されるのが望ましい。 This is to prevent strong heat from producing carcinogens such as trans fatty acids, saturated fats, and benzopyrene by minimizing the frictional heat that can be generated from the perilla in response to oil extraction pressure. It is desirable to understand that it is composed of.
前記沈殿段階Ｓ４００は、前記搾油段階３００を介して搾油されたエゴマ油を沈殿タンクに投入し、１分以上、自然沈殿させるように構成されている。 The precipitation step S400 is configured such that the perilla oil squeezed through the oil extraction step 300 is put into a settling tank and naturally settled for 1 minute or more.
前記冷低温処理段階Ｓ５００は、前記沈殿段階Ｓ４００を介して沈殿したエゴマ油が、エゴマ油が２５℃以下になるように冷低温処理する過程である。 The cold / low temperature treatment step S500 is a process in which the perilla oil precipitated through the precipitation step S400 is cold / low temperature treated so that the perilla oil becomes 25 ° C. or lower.
一方、付加的な面に沿って、前記冷低温処理段階Ｓ５００は、前記沈殿段階Ｓ４００を介して沈殿したエゴマ油では、下層に沈殿した沈殿層を除いた上層のエゴマ油のみを抽出する沈殿物除外エゴマ油の抽出段階後に遂行されるように構成することができる。 On the other hand, along the additional surface, the cold / low temperature treatment step S500 is a precipitate that extracts only the perilla oil in the upper layer excluding the settling layer precipitated in the lower layer in the perilla oil precipitated through the precipitation step S400. It can be configured to be carried out after the extraction stage of the excluded perilla oil.
前記冷低温処理段階Ｓ５００は、沈殿したエゴマ油を冷低温処理することにより、エゴマ油に含まれる様々な成分が、それぞれの融点以下に到達するようにすることによって、人為的にエゴマ油に含まれている様々な成分の凝固浮遊物が生成されるように構成される。 In the cold / low temperature treatment step S500, the precipitated perilla oil is artificially contained in the perilla oil by subjecting the precipitated perilla oil to a cold / low temperature treatment so that various components contained in the perilla oil reach below their respective melting points. It is configured to produce solidified suspended matter of various components.
この時、前記冷低温処理段階Ｓ５００は、前記沈殿物除外エゴマ油の抽出段階を経て、下層に沈殿した沈殿層を除いて、上層から抽出されたエゴマ油を冷低温処理することにより、エゴマ油に含まれる様々な成分が、それぞれの融点以下に到達するようにして、人為的にエゴマ油に含まれている様々な成分の凝固浮遊物が生成されるように構成されることができる。 At this time, in the cold / low temperature treatment step S500, the perilla oil extracted from the upper layer is subjected to the cold / low temperature treatment by removing the precipitate layer precipitated in the lower layer through the extraction step of the deposit-excluded perilla oil. It can be configured so that the various components contained in the perilla oil reach below their respective melting points to artificially generate coagulated suspended matter of various components contained in the perilla oil.
このとき、他の付加的な面に沿って、前記冷低温処理段階Ｓ５００の後に、低温処理されたエゴマ油を沈殿タンクに投入し、５分以上、自然沈殿させる沈殿段階Ｓ４００を再遂行するように構成されることができる。 At this time, along with other additional surfaces, after the cold / low temperature treatment step S500, the low temperature treated perilla oil is put into the settling tank, and the settling step S400 for spontaneous precipitation is performed again for 5 minutes or more. Can be configured in.
前記濾過段階Ｓ６００は、前記冷低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜５０μｍの食品用濾過網を利用して濾過させるように構成されている。 The filtration step S600 is configured to filter the perilla oil that has been subjected to the cold / low temperature treatment step S500 through a cold / low temperature treatment step S500 using a 1 to 50 μm food filtration net.
前記濾過段階Ｓ６００は、前記冷低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油から生成された凝固浮遊物を濾過して、今後、冷低温でもエゴマ油が凝固浮遊物なしでクリアな状態を維持できるように構成されている。 The filtration step S600 filters the coagulated suspended matter produced from the perilla oil that has been subjected to the cold / low temperature treatment through the cold / low temperature treatment step S500, and the perilla oil is in a state of being clear without the coagulated suspended matter even at a cold / low temperature in the future. It is configured to be able to maintain.
前記濾過段階Ｓ６００は、作業場の温度が２５℃以下になるように温度を設定した後に遂行されることによって、エゴマ油に含まれる様々な成分のそれぞれの融点以下である状態で濾過して、エゴマ油に含まれるさまざまな成分から生成された凝固浮遊物が融点に達していない状態で、すべて濾過されるように構成されるのが望ましい。 The filtration step S600 is carried out after setting the temperature so that the temperature of the workplace is 25 ° C. or lower, so that the perilla is filtered in a state of being equal to or lower than the melting point of each of the various components contained in the perilla oil. It is desirable that all the solidified suspensions produced from the various components contained in the oil have not reached the melting point and are all filtered.
一方、第３の実施例に係る本発明は、図２に示すように、エゴマの洗浄段階Ｓ１００、乾燥段階Ｓ２００、搾油段階Ｓ３００、沈殿段階Ｓ４００、冷低温処理段階 Ｓ５００、及び濾過段階Ｓ６００を含んで構成されている。 On the other hand, as shown in FIG. 2, the present invention according to the third embodiment includes a perilla washing step S100, a drying step S200, an oil extraction step S300, a precipitation step S400, a cold / low temperature treatment step S500, and a filtration step S600. It is composed of.
さらに詳細には、エゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法において、エゴマの洗浄器に投入して、５〜２０分間洗浄するエゴマの洗浄段階Ｓ１００と；前記エゴマの洗浄段階Ｓ１００を介して洗浄されたエゴマを水分率が４〜７％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させる乾燥段階Ｓ２００と；前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜４９℃の温度で圧力を加えて、５〜２０分間エゴマ油を搾油する搾油段階Ｓ３００と；前記搾油段階Ｓ３００を介して搾油されたエゴマ油を沈殿タンクに投入し、１０分以上、自然沈殿させる沈殿段階Ｓ４００と；前記沈殿段階Ｓ４００を介して沈殿させたエゴマ油が８℃以下になるように冷低温処理する冷低温処理段階Ｓ５００と；前記冷低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜１０μｍの食品用濾過網を利用して濾過させる濾過段階Ｓ６００と；を含んで構成されている。 More specifically, in the method for producing a cosmetic composition containing perilla oil beauty oil and vegetable omega-3, the perilla washing step S100, which is put into a perilla washer and washed for 5 to 20 minutes; A drying step S200 in which the perilla washed through the washing step S100 of the perilla is dried for 1 to 5 hours with cold and warm air at 15 to 80 ° C. so that the water content becomes 4 to 7%; The perilla dried perilla is put into an oil squeezer and pressure is applied at a temperature of 0 to 49 ° C. to squeeze the perilla oil for 5 to 20 minutes; the perilla oil squeezed through the oil squeezing step S300. A precipitation step S400 in which the oil is put into a precipitation tank and naturally precipitated for 10 minutes or more; a cold / low temperature treatment step S500 in which the perilla oil precipitated through the precipitation step S400 is subjected to cold / low temperature treatment so as to be 8 ° C. or lower. It is configured to include a filtration step S600 in which the perilla oil that has been subjected to the cold / low temperature treatment step S500 is filtered using a 1 to 10 μm food filter net.
具体的に説明すれば、前記のエゴマの洗浄段階Ｓ１００は、エゴマを洗浄器に投入して、５〜２０分間洗浄するように構成されている。 Specifically, the cleaning step S100 of the perilla is configured to put the perilla into a washing device and wash it for 5 to 20 minutes.
前記乾燥段階Ｓ２００は、エゴマの洗浄段階Ｓ１００を介して洗浄されたエゴマを水分率が４〜７％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させるように構成されている。 The drying step S200 is configured to dry the perilla washed through the washing step S100 of the perilla with cold and warm air at 15 to 80 ° C. for 1 to 5 hours so that the moisture content is 4 to 7%. There is.
前記乾燥段階Ｓ２００は、１５〜８０℃の冷温風で１〜５時間乾燥させるように構成されるが、エゴマの量に応じて、乾燥時間を適切に調節して、水分率が４〜７％となるように構成されるのが好ましく、絶対的な時間ではなく、水分率を重要事項として置いて、水分率に基づいて、乾燥時間を調整して遂行するように構成されるのが望ましい。 The drying step S200 is configured to be dried with cold / warm air at 15 to 80 ° C. for 1 to 5 hours, but the drying time is appropriately adjusted according to the amount of perilla, and the moisture content is 4 to 7%. It is preferable that the drying time is adjusted based on the moisture content, with the moisture content as an important matter, not the absolute time.
前記乾燥段階Ｓ２００は、洗浄されたエゴマの水分率を下げることにより、抽出収率を高めるだけでなく、エゴマを熱風で炒めることにより、搾油されたエゴマ油の香ばしい香りと風味を生かすことができるように構成されている。 In the drying step S200, not only the extraction yield is increased by lowering the water content of the washed perilla, but also the fragrant aroma and flavor of the squeezed perilla oil can be utilized by frying the perilla with hot air. It is configured as follows.
前記乾燥段階Ｓ２００は、水分率が４〜７％になるようにすることによって、エゴマの表皮とエゴマの中身との間の水分を乾燥し、エゴマの表皮との間の水分を乾燥させて、エゴマの粒子が等しく配列されるようにすることによって、搾油時、エゴマ粒子が圧力を均一に受けるようにして抽出収率を高めることができるように構成されている。 In the drying step S200, the water content between the perilla skin and the contents of the perilla is dried by adjusting the water content to 4 to 7%, and the water between the perilla skin and the perilla skin is dried. By making the perilla particles evenly arranged, it is configured so that the perilla particles are uniformly subjected to pressure during oil extraction and the extraction yield can be increased.
前記乾燥段階Ｓ２００は、１５〜８０℃の冷温風で１〜５時間乾燥することにより、強い熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されている。 The drying step S200 is configured to prevent the production of carcinogens such as trans fatty acids, saturated fats, and benzopyrene by strong heat by drying with cold and warm air at 15 to 80 ° C. for 1 to 5 hours. ing.
この時、前記乾燥段階Ｓ２００は、エゴマが等しく混合されるように攪拌する攪拌段階と一緒に遂行されるように構成されることができる。 At this time, the drying step S200 can be configured to be carried out together with a stirring step of stirring so that the perilla is equally mixed.
したがって、前記乾燥段階Ｓ２００において、前記攪拌段階が同時に遂行されることによって、熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止することができるようになる。 Therefore, in the drying step S200, by simultaneously performing the stirring step, it becomes possible to prevent the production of carcinogens such as trans fatty acids, saturated fats, and benzopyrene by heat.
そして、前記搾油段階Ｓ３００は、前記乾燥段階Ｓ２００を介して乾燥されたエゴマを搾油器に投入し、０〜４９℃の温度で圧力を加えて、５〜２０分間エゴマ油を搾油するように構成されている。 Then, the oil extraction step S300 is configured to put the perilla dried through the drying step S200 into an oil press and apply pressure at a temperature of 0 to 49 ° C. to squeeze the perilla oil for 5 to 20 minutes. Has been done.
前記搾油段階Ｓ３００は、４００〜７００ｋｇｆ／ｃｍ２の圧力で搾油することができる。 The oil extraction step S300 can be extracted at a pressure of 400 to 700 kgf / cm 2 .
前記搾油段階Ｓ３００は、作業場の温度が０〜４９℃の範囲内になるように温度を設定したあと遂行されることによって、強力な熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されている。 The oil extraction step S300 is carried out after setting the temperature so that the temperature of the workplace is in the range of 0 to 49 ° C., so that strong heat produces carcinogens such as trans fatty acids, saturated fats, and benzopyrene. It is configured to prevent this from happening.
前記搾油段階Ｓ３００は、エゴマの量に応じて搾油時間を適切に調節するように構成されるのが望ましい。 It is desirable that the oil extraction step S300 is configured so that the oil extraction time is appropriately adjusted according to the amount of perilla.
前記搾油段階Ｓ３００は、例えば、３００ｋｇｆ／ｃｍ２まで搾油圧力を継続的に上昇させた後、３００ｋｇｆ／ｃｍ２の圧力に到達すれば、５分間搾油圧力を１０〜５０ｋｇｆ／ｃｍ２の範囲内で段階的に上昇させることによって、時間に応じて段階的に７００ｋｇｆ／ｃｍ２まで搾油圧力を上昇させるように構成することができる。 The oil extraction step S300, for example, after continuously increasing the oil extraction pressure to 300 kgf / cm 2, when reaching a pressure of 300 kgf / cm 2, for 5 minutes expeller pressure in the range of 10~50kgf / cm 2 By gradually increasing the oil extraction pressure, the oil extraction pressure can be increased stepwise up to 700 kgf / cm 2 according to the time.
これは、搾油圧力に応じてエゴマから発生する可能性のある摩擦熱を最小限に抑えることによって、強い熱によりトランス脂肪酸、飽和脂肪、及びベンゾピレンなどの発癌物質が生成されることを防止するように構成されているものと理解されることが望ましい。 This is to prevent strong heat from producing carcinogens such as trans fatty acids, saturated fats, and benzopyrene by minimizing the frictional heat that can be generated from the perilla in response to oil extraction pressure. It is desirable to be understood as being configured in.
そして、前記沈殿段階Ｓ４００は、前記搾油段階３００を介して搾油されたエゴマ油を沈殿タンクに投入し、１０分以上、自然沈殿させるように構成されている。 Then, the precipitation step S400 is configured such that the perilla oil squeezed through the oil extraction step 300 is put into a settling tank and naturally settled for 10 minutes or more.
そして、前記冷低温処理段階Ｓ５００は、沈殿段階Ｓ４００を介して沈殿させたエゴマ油が８℃以下になるように冷低温処理することになる。 Then, in the cold / low temperature treatment step S500, the cold / low temperature treatment is performed so that the perilla oil precipitated through the precipitation step S400 is 8 ° C. or lower.
前記冷低温処理段階Ｓ５００は、前記沈殿段階Ｓ４００を介して沈殿したエゴマ油では、下層に沈殿した沈殿層を除いた上層のエゴマ油のみを抽出する沈殿物除外エゴマ油の抽出段階の後に遂行されるように構成することができる。 The cold / low temperature treatment step S500 is performed after the precipitation-excluded perilla oil extraction step, which extracts only the upper layer of perilla oil excluding the sedimentation layer precipitated in the lower layer of the perilla oil precipitated through the precipitation step S400. Can be configured to
前記冷低温処理段階Ｓ５００は、沈殿したエゴマ油を冷低温処理することにより、エゴマ油に含まれる様々な成分が、それぞれの融点以下に到達するようにすることによって、人為的にエゴマ油に含まれている様々な成分の凝固浮遊物が生成されるように構成される。 In the cold / low temperature treatment step S500, the precipitated perilla oil is artificially contained in the perilla oil by subjecting the precipitated perilla oil to a cold / low temperature treatment so that various components contained in the perilla oil reach below their respective melting points. It is configured to produce solidified suspended matter of various components.
この時、前記冷低温処理段階Ｓ５００は、前記沈殿物除外エゴマ油の抽出段階を経て、下層に沈殿した沈殿層を除いて、上層から抽出されたエゴマ油を低温処理することにより、エゴマ油に含まれる様々な成分が、それぞれの融点以下に到達するようにして、人為的にエゴマ油に含まれている様々な成分の凝固浮遊物が生成されるように構成されることができる。 At this time, in the cold / low temperature treatment step S500, the perilla oil extracted from the upper layer is subjected to the extraction step of the sediment-excluded perilla oil, and the perilla oil extracted from the upper layer is subjected to the low temperature treatment to obtain the perilla oil. The various components contained can be configured to reach below their respective melting points so that coagulated suspensions of the various components contained in the perilla oil are artificially produced.
この時、前記冷低温処理段階Ｓ５００の後に、冷低温処理されたエゴマ油を沈殿タンクに投入し、５分以上、自然沈殿させる沈殿段階を再遂行するように構成されることができる。 At this time, after the cold / low temperature treatment step S500, the cold / low temperature treated perilla oil can be put into the settling tank, and the settling step of spontaneous precipitation can be performed again for 5 minutes or more.
そして、前記濾過段階Ｓ６００は、前記冷低温処理段階Ｓ５００を介して冷低温処理されたエゴマ油を１〜１０μｍの食品用濾過網を利用して濾過させるように構成されている。 Then, the filtration step S600 is configured to filter the perilla oil that has been subjected to the cold / low temperature treatment step S500 through the cold / low temperature treatment step S500 using a 1 to 10 μm food filtration net.
前記濾過段階Ｓ６００は、前記冷低温処理段階Ｓ５００を介して低温処理されたエゴマ油から生成された凝固浮遊物を濾過して、今後、低温でもエゴマ油が凝固浮遊物なしでクリアな状態を維持できるように構成されているものである。 The filtration step S600 filters the coagulated suspended matter produced from the perilla oil treated at low temperature through the cold / low temperature treatment step S500, and the perilla oil will maintain a clear state without the coagulated suspended matter even at a low temperature in the future. It is configured so that it can be done.
前記濾過段階Ｓ６００は、作業場の温度が１０℃以下になるように温度を設定した後に遂行されることによって、エゴマ油に含まれている様々な成分の各々が融点以下状態で濾過して、エゴマ油に含まれるさまざまな成分から生成された凝固浮遊物が融点に達していない状態で、すべて濾過されるように構成されるのが望ましい。 The filtration step S600 is carried out after setting the temperature so that the temperature of the workplace is 10 ° C. or lower, so that each of the various components contained in the perilla oil is filtered in a state of being below the melting point, and the perilla is filtered. It is desirable that all the solidified suspensions produced from the various components contained in the oil have not reached the melting point and are all filtered.
以下、本発明の一実施形態に基づいて、実施例を通じて本発明をより具体的に説明するが、これは本発明の特異な目的をより明確にし、本発明による顕著な効果をより容易に理解できるようにするための例示であるだけで、本発明の実施において、その範囲を制限することがないので、本発明の権利範囲を制限しないものと理解されるのが望ましい。 Hereinafter, the present invention will be described in more detail through examples based on one embodiment of the present invention, which clarifies the peculiar purpose of the present invention and more easily understands the remarkable effect of the present invention. It is desirable that it is understood that it does not limit the scope of rights of the present invention because it is merely an example for enabling the present invention and does not limit the scope thereof in the practice of the present invention.
実施例
エゴマ２０ｋｇを洗浄器に投入し、１５分間洗浄し、ふるいを利用して１０分間水気を除去した後、洗浄されたエゴマを水分率が５％になるまで、３０℃の冷温風でエゴマを均等に混ぜて撹拌し、炒めて乾燥させた。以降、作業場の温度を２５℃に設定し、乾燥したエゴマを搾油器に投入し、搾油器の搾油圧力を４００ｋｇｆ／ｃｍ２まで継続的に上昇させて搾油し、４００ｋｇｆ／ｃｍ２ の搾油圧力の後に、５分間搾油圧力を５０ｋｇｆ／ｃｍ２ずつ上昇させ、搾油圧力が６００ｋｇｆ／ｃｍ２に到達するまで、約２０分間さらに加圧しながら搾油した。以降、搾油されたエゴマ油を沈殿タンクに投入し、沈殿層が生成されるまで約３０分間自然沈殿させ、沈殿層の上層に形成されたエゴマ油だけを抽出して抽出されたエゴマ油が１０℃以下になるまで冷低温処理した後、作業場の温度を１０℃に設定し、冷低温処理されたエゴマ油を５μｍの食品用濾過網に濾過して、濾過されたエゴマ油を容器に充填した。以後、容器に充填されたエゴマ油を冷蔵庫で１０時間程度保管した後、下記のように官能検査を実施した。
Example 20 kg of perilla is put into a washing machine, washed for 15 minutes, and after removing water for 10 minutes using a sieve, the washed perilla is kept at 30 ° C. until the water content reaches 5%. The perilla was mixed evenly with cold and warm air, stirred, fried and dried. Later, to set the temperature of the workplace to 25 ° C., the dried green perilla were placed in oil extraction unit, continuously crush by increasing the oil extraction pressure of oil extraction instrument to the 400 kgf / cm 2, the crush pressure of 400 kgf / cm 2 after the 5 minutes crush pressures ramped at 50 kgf / cm 2, expeller pressure until it reaches the 600 kgf / cm 2, and crush while further pressurized to about 20 minutes. After that, the squeezed egoma oil is put into a settling tank and naturally precipitated for about 30 minutes until a settling layer is formed, and only the egoma oil formed on the upper layer of the settling layer is extracted to obtain 10 egoma oils. After cold-low temperature treatment until the temperature became below ° C, the temperature of the workplace was set to 10 ° C, the cold-low temperature treated egoma oil was filtered through a 5 μm food filter net, and the filtered egoma oil was filled in a container. .. After that, the perilla oil filled in the container was stored in the refrigerator for about 10 hours, and then the sensory test was carried out as follows.
比較例１
ゴマとエゴマを粉末化して、トウモロコシ油に投入し、加熱して油を抽出し、これを通常のエゴマ油の抽出方法に代入してエゴマ油を搾油するが、粉末化する段階を省略してエゴマ油を製造した。
Comparative Example 1
The sesame and perilla are pulverized, put into corn oil, heated to extract the oil, and this is substituted into the usual method of extracting perilla oil to squeeze the perilla oil, but the step of pulverizing is omitted. Produced perilla oil.
エゴマ２０ｋｇを１９０℃の温度で１０分間熱を加えた後、遠心分離器で１次濾過して固形分を除去した後、フィルタープレスで２次濾過して、微細な固形分を除去し、室温で冷却した後、ポリフェノックス６ｇを添加して容器にエゴマ油を充填した。以後、容器に充填されたエゴマ油を冷蔵庫で１０時間程度保管した後、下記のように官能評価を実施した。 After heating 20 kg of perilla at a temperature of 190 ° C. for 10 minutes, the perilla is first filtered with a centrifuge to remove solids, and then secondarily filtered with a filter press to remove fine solids at room temperature. After cooling with, 6 g of polyphenox was added and the container was filled with perilla oil. After that, the perilla oil filled in the container was stored in the refrigerator for about 10 hours, and then the sensory evaluation was carried out as follows.
比較例２
エゴマ油中の飽和脂肪酸を除去した食用油の製造方法に基づいてエゴマ油を製造した。
Comparative Example 2
Perilla oil was produced based on the method for producing edible oil from which saturated fatty acids in perilla oil were removed.
１０５℃で加熱したシリコーンオイルバス（ｓｉｌｉｃｏｎｅ−ｏｉｌ ｂａｔｈ）で約３０分間減圧して、徹底的に脱水したエゴマ油５０．３３ｇｍをとり、無水エタノール２５０ｍｌと混合し、２８％−ナトリウム‐メトキシド（ｓｏｄｉｕｍ−ｍｅｔｈｏｘｉｄｅ）４００μｌを添加した後に磁気攪拌器で約５時間攪拌して、トランスエステリフィケーション反応を経た油層とエタノール層の界面がなくなった透明な溶液を獲得した。反応を停止させるために使用されたナトリウム‐メトキシドについて氷酢酸１１０μｌを加え中和し、別に尿素（ｕｒｅａ）８０ｇｍを無水エタノール２５０ｍｌに加熱溶解させた溶液を混合した後に、保温してなるべく徐々に冷却させながら一晩放置した。以後、飽和脂肪酸のエチルエステルが尿素複合体を形成して結晶に析出されば、結晶を吸引及び濾過して除去し、その濾液を減圧濃縮して無水エタノールを回収してリサイクルするようにして濃縮した後、残留物に少量の水とｄ−ＨＣｌを加え、不飽和脂肪酸の尿素−複合体を破壊させて不飽和脂肪酸を析出させた後、ヘキサンで抽出し、ヘキサンを濃縮して不飽和脂肪酸のエチル−エステルを獲得し、得られた不飽和脂肪酸に対してエチルエステルをよく乾燥させた後、トリセチン（ｇｌｙｃｅｒｉｎ−ｔｒｉａｃｅｔａｔｅ）１０．５５ｇｍと２８％ナトリウム‐メトキシド溶液４００μｌを混合し、インターエステリフィケーション反応によって不飽和脂肪酸のトリグリセリドを再合成するためにシリコーンオイルバス中で９０〜１２５℃で加熱しながら５０ｍｍ／Ｈｇ以下に減圧してインターエステリフィケーション反応によりエチルアセテート（ｅｔｈｙｌ−ａｃｅｔａｔｅ）を除去し、不飽和脂肪酸だけで構成されたトリグリセリドを獲得し、反応が完結して、もはや気泡発生がないとき、反応物に１１０μｌの氷酢酸を加えて中和し、水を加えた後にヘキサンで繰り返し抽出し、濃縮して飽和脂肪酸を除去し、不飽和脂肪酸だけで構成されたエゴマ油を抽出して容器にエゴマ油を充填した。以後、容器に充填されたエゴマ油を冷蔵庫で１０時間程度保管した後、下記のように官能評価を実施した。 Reduce the pressure in a silicone oil bath heated at 105 ° C. for about 30 minutes to take 50.33 gm of thoroughly dehydrated egoma oil, mix with 250 ml of absolute ethanol, and 28% -sodium-methoxide (sodium). After adding 400 μl of −methoxide), the mixture was stirred with a magnetic stirrer for about 5 hours to obtain a transparent solution in which the interface between the oil layer and the ethanol layer that had undergone the transestheticization reaction had disappeared. 110 μl of glacial acetic acid was added to neutralize the sodium-methoxide used to stop the reaction, and a solution of 80 gm of urea (urea) dissolved in 250 ml of absolute ethanol by heating was mixed, followed by heat retention and cooling as gradually as possible. I left it overnight. After that, when the ethyl ester of the saturated fatty acid forms a urea complex and is precipitated in the crystal, the crystal is removed by suction and filtration, and the filtrate is concentrated under reduced pressure to recover anhydrous ethanol and concentrated by recycling. After that, a small amount of water and d-HCl were added to the residue to destroy the urea-complex of unsaturated fatty acids to precipitate unsaturated fatty acids, and then extracted with hexane, and the hexane was concentrated to concentrate unsaturated fatty acids. After obtaining the ethyl-ester of the above and drying the ethyl ester well against the obtained unsaturated fatty acid, 10.55 gm of tricetin (glycerin-triacete) and 400 μl of a 28% sodium-methoxyde solution are mixed and interesterify. In order to resynthesize the triglyceride of unsaturated fatty acid by the application reaction, ethyl acetate is removed by the interesthetic reaction reaction by reducing the pressure to 50 mm / Hg or less while heating at 90 to 125 ° C. in a silicone oil bath. Then, when the triglyceride composed only of unsaturated fatty acids is obtained and the reaction is completed and no bubbles are generated anymore, 110 μl of glacial acetic acid is added to the reaction product to neutralize it, water is added, and then the reaction is repeated with hexane. It was extracted and concentrated to remove saturated fatty acids, and egoma oil composed only of unsaturated fatty acids was extracted and the container was filled with egoma oil. After that, the perilla oil filled in the container was stored in the refrigerator for about 10 hours, and then the sensory evaluation was carried out as follows.
比較例３
エゴマ２０ｋｇを１８℃の冷水で洗浄し、ふるいを利用して水気を除去した。作業場者の温度を１９℃以下に設定し、除湿機を使用して、相対湿度を２０％に維持するようにした。１５ないし１６℃に設定された冷風器を利用して、約３時間の間にエゴマを十分に乾燥した。冷風の乾燥時、エゴマを手でもんで簡単に壊れているか確認し、乾燥されたエゴマをポリエステル製のメッシュ構造を有する布に入れて、５８０ｋｇｆ／ｃｍ２の圧力で１０分間予圧した後、超音波振動を加え、再び６００ｋｇｆ／ｃｍ２の圧力で加圧して油を絞る。得られた油を容器に充填して、冷蔵庫に１０時間程度保管した後、下記のように官能評価を実施した。
Comparative Example 3
20 kg of perilla was washed with cold water at 18 ° C., and water was removed using a sieve. The temperature of the workers was set to 19 ° C. or lower, and a dehumidifier was used to maintain the relative humidity at 20%. The perilla was sufficiently dried in about 3 hours using a cool air blower set at 15 to 16 ° C. When the cold air dries, check if the perilla is easily broken by hand, put the dried perilla in a cloth with a polyester mesh structure, prepress it at a pressure of 580 kgf / cm 2 for 10 minutes, and then ultrasonic waves. Add vibration and pressurize again with a pressure of 600 kgf / cm 2 to squeeze the oil. The obtained oil was filled in a container and stored in a refrigerator for about 10 hours, and then a sensory evaluation was carried out as follows.
比較例４
エゴマ２０ｋｇを２％濃度の炭酸水素ナトリウムの混合溶液で３５℃の温度で５分間攪拌して洗浄した後、高速脱水機を用いて、エゴマの水分含水量が１５％になるように乾燥した。以後、炒め釜を３１０℃の温度に維持して、エゴマを２００℃の温度で２０分間加熱して炒めた後、９５℃の温度で６３０ｋｇｆ／ｃｍ２の圧力を加えてエゴマ油を製造した。続いて、搾油されたエゴマ油に５０メッシュ（ｍｅｓｈ）の粒子の大きさに粉砕された堅炭の活性炭粉末２００ｇを混合した後、３５分間攪拌し、堅炭の活性炭粉末が混合されたエゴマ油を０．１μｍのプレスフィルターを用いて濾過した。容器に濾過されたエゴマ油を充填し、容器に充填されたエゴマ油を冷蔵庫で１０時間程度保管した後、下記のように官能評価を実施した。
Comparative Example 4
After washing 20 kg of perilla with a mixed solution of 2% sodium hydrogen carbonate at a temperature of 35 ° C. for 5 minutes, the perilla was dried using a high-speed dehydrator so that the water content of the perilla was 15%. After that, the frying pot was maintained at a temperature of 310 ° C., the perilla was heated at a temperature of 200 ° C. for 20 minutes to fry, and then a pressure of 630 kgf / cm 2 was applied at a temperature of 95 ° C. to produce perilla oil. Subsequently, 200 g of hard charcoal activated carbon powder crushed to a particle size of 50 mesh (mesh) was mixed with the squeezed perilla oil, and then stirred for 35 minutes to remove the perilla oil mixed with the hard charcoal activated carbon powder. . Filtered using a 1 μm press filter. The container was filled with filtered perilla oil, and the perilla oil filled in the container was stored in a refrigerator for about 10 hours, and then a sensory evaluation was carried out as follows.
＜官能評価１＞
前記の“実施例”と“比較例１”ないし“比較例４”を使用して製造されたエゴマ 油の味、香り、視覚的選好度、総合選好度等について官能評価を実施し、その結果を下記の［表２］に示し、官能評価は、食品関連の専門家と一般消費者５０人を対象にして９点の採点法を利用して実施し、下記の［表１］に示した。
<Sensory evaluation 1>
Sensory evaluation was performed on the taste, aroma, visual preference, overall preference, etc. of the egoma oil produced using the above-mentioned "Example" and "Comparative Example 1" to "Comparative Example 4", and the result was obtained. Was shown in [Table 2] below, and the sensory evaluation was performed using a 9-point scoring method for 50 food-related experts and general consumers, and is shown in [Table 1] below. ..
味：エゴマ油の香ばしい味が生きており、苦味が最小に感じられるときに、９点満点とし、官能評価を実施する。 Taste: When the fragrant taste of perilla oil is alive and the bitterness is felt to the minimum, a maximum of 9 points is given and a sensory evaluation is performed.
香り：エゴマ油の香ばしい香りが生きており、生臭いにおいが最小限に感じられるときに、９点満点とし、官能評価を実施する。 Fragrance: When the fragrant scent of perilla oil is alive and the fishy odor is felt to the minimum, a maximum of 9 points is given and a sensory evaluation is performed.
視覚的選好度：肉眼で観察して残渣が観察されていないクリアな状態であり、透明な色が感じられるときを９点満点とし、官能評価を実施する。 Visual preference: A sensory evaluation is performed with a maximum of 9 points when a clear state is observed with the naked eye and no residue is observed and a transparent color is felt.
総合選好度：味、香り、視覚的選好度を総合的に判断して、総合的に優れていたときには、９点満点とし、官能評価を実施する。 Overall Preference: Taste, aroma, and visual preference are comprehensively judged, and if the overall preference is excellent, a maximum of 9 points is given and a sensory evaluation is performed.
これは、１５〜８０℃の冷温風で１〜５時間乾燥してエゴマを撹拌し、乾燥することにより、エゴマがエゴマ油の製造に適した状態になることによって、搾油時エゴマ油の風味と香りが存続し、搾油されたエゴマ油を自然沈殿および冷低温処理して、沈殿物及び凝固浮遊物を濾過することにより、透明なエゴマ油を製造して、冷蔵保管しても凝固される浮遊物が発生していないので、消費者の選好度を倍加させたからであると判断される。 This is because the perilla is dried with cold and warm air at 15 to 80 ° C. for 1 to 5 hours, the perilla is agitated, and the perilla is in a state suitable for the production of perilla oil. A clear perilla oil is produced by natural precipitation and cold / low temperature treatment of the squeezed perilla oil, and the sediment and coagulated perilla are filtered, and the perilla oil is coagulated even when stored in a refrigerator. It is judged that this is because the product has not been generated and the consumer's preference has been doubled.
最後に、前記のような実施例１を介して製造されたエゴマ油が含有された化粧料組成物を化粧品全体の重量に対して０．００１〜３０．０重量部含有されるようにして化粧品を製造することならば、本発明で提示された効果を提供することができるようになる。 Finally, the cosmetic composition containing perilla oil produced through Example 1 as described above is contained in an amount of 0.001 to 3.0 parts by weight based on the total weight of the cosmetic. The production of the above makes it possible to provide the effects presented in the present invention.
＜ベンゾピレン含有量照射＞
ベンゾピレン（Ｂｅｎｚｏｐｙｒｅｎｅ）は、多環芳香族炭化水素（ＰＡＨ：Ｐｏｌｙｃｙｃｌｉｃ Ａｒｏｍａｔｉｃ Ｈｙｄｒｏｃａｒｂｏｎ）の一種であり、人体に蓄積された場合、様々な癌を誘発し突然変異を起こす環境ホルモンとして、食品のベンゾピレン（ｂｅｎｚｏｐｙｒｅｎｅ）の生成は、食品を加熱する過程から生成されることができるし、食品を高温で調理したり、加工時の食品の主成分である炭水化物、タンパク質、脂肪などが不完全燃焼されて生成されることができる。
<Irradiation of benzopyrene content>
Benzopyrene is a type of polycyclic aromatic hydrocarbon (PAH) that, when accumulated in the human body, is a food benzopyrene as an environmental hormone that induces various cancers and causes mutations. ) Can be produced from the process of heating foods, and is produced by cooking foods at high temperatures or by incompletely burning carbohydrates, proteins, fats, etc., which are the main components of foods during processing. Can be
前記の「実施例」及び「比較例１」ないし「比較例４」を使用して製造されたエゴマ油のベンゾピレン含量に対して食品医薬品安全処で告示した実験方法で含有量を 照射し、実験装置は、ＹＯＵＮＧＬＩＮ機器株式会社の高速液体クロマトグラフィー（ＭＯＤＥＬ：ＳＰ９２５Ｓ）、蛍光検出器は、ＪＡＳＣＯＦＰ−２０２０ＰＬＵＳ、オートサンプラーを使用し、内部の物質として３−メチルコラントレンを使用してＮ、Ｎ−ジメチルホルムアミド−水（９：１）とヘキサンで抽出した後、ＳＰＥ（Ｓｏｌｉｄ Ｐｈａｓｅ Ｅｘｔｒａｃｔｉｏｎ）カートリッジで精製して、高速液体クロマトグラフィー／蛍光検出器で分析し、分析された結果を下記の［表３］に示す。 The benzopyrene content of the egoma oil produced using the above-mentioned "Example" and "Comparative Example 1" to "Comparative Example 4" was irradiated with the content by the experimental method notified by the Food and Drug Administration, and the experiment was conducted. The device uses high performance liquid chromatography (MODEL: SP925S) of YOUNGLIN Equipment Co., Ltd., the fluorescence detector uses JASCOFP-2020PLUS, an autosampler, and 3-methylcholanthrene is used as the internal substance. After extraction with dimethylformamide-water (9: 1) and hexane, it was purified with an SPE (Solid Phase Laboratory) cartridge, analyzed with a high performance liquid chromatography / fluorescence detector, and the results of the analysis were shown in [Table 3] below. ] Shown.
前記［表３］を参照すれば、「実施例」に基づいて製造されたエゴマ油組成物の場合には、ベンゾピレンの検出量は、法的基準値である２．０ｐｐｂの以下として、基準値をずっと下回る優れたレベルに判定され、これは、弱い熱風即ち、１５〜８０℃の冷温風を利用して、エゴマを撹拌し、乾燥することにより、抽出収率が優れているだけでなく、強力な熱により有害物質が生成されることを最小限に抑えたからであると判断される。 With reference to the above [Table 3], in the case of the perilla oil composition produced based on "Example", the detected amount of benzopyrene is set to be less than or equal to the legal standard value of 2.0 ppb, which is the standard value. It was judged to be an excellent level well below, which is not only excellent in extraction yield by stirring and drying the perilla using weak hot air, that is, cold air at 15-80 ° C. It is judged that this is because the production of harmful substances by strong heat was minimized.
しかしながら、前記「比較例１」、「比較例２」および「比較例４」は、強い熱により加工されることによって、項目の判定として不適合判定を受け、食品として摂取するには不適合であることがわかった。 However, the above-mentioned "Comparative Example 1", "Comparative Example 2" and "Comparative Example 4" are processed by strong heat and are judged to be non-conforming as an item judgment, and are non-conforming to be ingested as food. I understood.
一方、「比較例３」は、ベンゾピレンの検出量が０．３ｐｐｂで最も少なく検出されたが、「比較例３」は、生エゴマから抽出したエゴマ油であるだけに、官能検査では、味と香りが、本発明の「実施例」に従って製造されたエゴマ油組成物に比べて低い。よって、本発明に基づいて化粧品に配合されているエゴマ油組成物を製造することが、従来の案出された他の技術に比べて味、香り、視覚的評価、総合評価、食品としての無害性などを総合的に判断した場合に最も適していることが分かった。 On the other hand, in "Comparative Example 3", the amount of benzopyrene detected was the smallest at 0.3 ppb, but in "Comparative Example 3", since it was perilla oil extracted from raw perilla, the sensory test showed the taste. The aroma is lower than that of the perilla oil composition produced according to "Examples" of the present invention. Therefore, producing the perilla oil composition blended in cosmetics based on the present invention is harmless as a food, taste, aroma, visual evaluation, comprehensive evaluation, as compared with other conventionally devised techniques. It was found that it is most suitable when the sex is comprehensively judged.
一方、図３ないし図４を参照すれば、図３ないし図４は、現在市販されている従来の製造方法によって製造されたエゴマ油の常温保管時の様子と、同じエゴマ油の冷蔵保管時の様子とを示す写真図である。図３ないし図４に示すように、従来の製造方法により製造されたエゴマ油は、冷蔵保管時、凝固・結露・凍結の浮遊物が発生していることを肉眼でも容易に確認することができる。即ち、黒塗りの部分をガラスびん後側に位置させた場合に、黒に着色された部分が見えないほどの凝固・結露・凍結の浮遊物がひどく発生したことを確認することができる。 On the other hand, referring to FIGS. 3 to 4, FIGS. 3 to 4 show the state of the perilla oil produced by the conventional manufacturing method currently on the market at room temperature and the state of the same perilla oil during refrigeration storage. It is a photographic figure which shows the state. As shown in FIGS. 3 to 4, the perilla oil produced by the conventional production method can be easily confirmed with the naked eye that coagulation, dew condensation, and frozen suspended matter are generated during refrigerated storage. .. That is, when the black-painted portion is positioned on the rear side of the glass bottle, it can be confirmed that the floating matter of solidification, dew condensation, and freezing is so severely generated that the black-colored portion cannot be seen.
一方、図５を参照すれば、図５は、本発明の製造方法に応じて、化粧品に配合されているエゴマ油組成物を製造したときの様子を示す写真図であり、付着番号１〜５は、本発明の製造段階中、冷低温処理段階を経ずに沈殿段階と濾過段階だけ経た後に、冷蔵保管したエゴマ油の様子を示すものである。沈殿段階と濾過段階とを経ても、エゴマ油に含まれる様々な成分が凝固、凝結、凍結され、浮遊物が発生している様子が成分に基づいてそれぞれ異なって現れるので、澄んだエゴマ油を得るためには、低温処理段階が不可欠な段階であることがわかり、付着番号６は、冷低温処理工程を含む本発明の製造方法により製造が完成されたエゴマ油の様子を示すものであり、付着番号６のエゴマ油は、冷蔵保管しても、図面のように凝固浮遊物なしですっきりとした状態を維持していることを確認できる。総合的に分析したとき、冷低温処理段階が重要な段階として、きれいですっきりとしたエゴマ油を得るために必須的に冷低温処理段階を実行する必要があることがわかった。 On the other hand, with reference to FIG. 5, FIG. 5 is a photographic view showing a state when the perilla oil composition blended in the cosmetics is produced according to the production method of the present invention, and the adhesion numbers 1 to 5 are shown. Shows the state of perilla oil stored in a refrigerated state after undergoing only a precipitation step and a filtration step without going through a cold / low temperature treatment step during the production step of the present invention. Even after going through the precipitation stage and the filtration stage, the various components contained in the perilla oil are coagulated, condensed, frozen, and the appearance of suspended matter is generated differently depending on the components, so clear perilla oil should be used. It was found that the low-temperature treatment step was an indispensable step in order to obtain the perilla oil, and the adhesion number 6 indicates the state of the perilla oil whose production was completed by the production method of the present invention including the cold-low temperature treatment step. It can be confirmed that the perilla oil having the adhesion number 6 maintains a neat state without solidified suspended matter as shown in the drawing even when stored in a refrigerator. Comprehensive analysis revealed that the cold and low temperature treatment step was an important step and that the cold and low temperature treatment step was essential to obtain a clean and clean perilla oil.
一方、一般的に、エゴマ油は「酸敗が速い」という認識で冷蔵保管を勧めているが、エゴマ油を低温（冷蔵庫温度程度）で保管する時、凝固や結露が生じ、消費者は、製品が腐敗したり、異常があるものと考えることができる。 On the other hand, in general, we recommend refrigerating perilla oil because it recognizes that it has a fast rancidity. However, when perilla oil is stored at a low temperature (about the refrigerator temperature), coagulation and dew condensation occur, and consumers are asked to use the product. Can be considered to be rotten or abnormal.
したがって、本発明は、このような現象を除去するための技術として、低温で保管しても、消費者に澄んだエゴマ油を提供することができる技術である。 Therefore, the present invention is a technique for removing such a phenomenon, which can provide consumers with clear perilla oil even when stored at a low temperature.
従来のエゴマ油に加えて、その他の穀物、例えば、ティーシードオイルを凍って結晶化させるタンクに入れ、６℃で８時間の間維持する技術が存在しているが、エゴマ油の場合には、凍らせて結晶化するべきでない。 In addition to conventional perilla oil, there is a technique for placing other grains, such as tea seed oil, in a freezing and crystallizing tank and maintaining it at 6 ° C for 8 hours, but in the case of perilla oil, , Should not be frozen and crystallized.
即ち、ティーシードオイルは、前記のように凍らせて結晶化する過程が必要であるが、本発明のエゴマ油は、凍らせて結晶化する過程があってはならない。 That is, the tea seed oil needs a process of freezing and crystallizing as described above, but the perilla oil of the present invention must not have a process of freezing and crystallizing.
具体的には、エゴマ油を冷凍すると、完全に固く結晶化し固体となるため、使用できない。即ち、１０℃程度でよく溶けず、高い３０℃以上の熱を加えなければ、液体化せずフィルタリング処理することが困難な問題が発生することになる。 Specifically, when perilla oil is frozen, it cannot be used because it crystallizes completely and becomes a solid. That is, it does not melt well at about 10 ° C., and unless high heat of 30 ° C. or higher is applied, there arises a problem that it is difficult to perform filtering without liquefying.
したがって、エゴマ油で低温（冷蔵温度）で生じる凝固現象を除去するためには、２５℃以下で、好ましくは１０℃以下で凝固させなければならない。 Therefore, in order to remove the coagulation phenomenon that occurs at a low temperature (refrigerating temperature) with perilla oil, it must be coagulated at 25 ° C. or lower, preferably 10 ° C. or lower.
この時には、固化ではなく、ゲルの形態で脂肪酸が凝固されて白い斑点などが発生することになる。前記ゲルの形態は若干の物理的圧力を加えると、すぐにゲルがなくなるが、例えば、指で揉んだ場合、すぐになくなる程度である。 At this time, fatty acids are coagulated in the form of gel instead of solidification, and white spots and the like are generated. The form of the gel disappears immediately when a slight physical pressure is applied, but it disappears immediately when rubbed with a finger, for example.
また、冷低温処理では、固化させると、濾過自体ができない。よって、固体を分解するためには、高い熱を加えなければならないので、従来の技術とは異なる製造プロセスを持つことになる。 Further, in the cold / low temperature treatment, when solidified, filtration itself cannot be performed. Therefore, in order to decompose the solid, high heat must be applied, so that the manufacturing process is different from that of the conventional technology.
そして、本発明の濾過段階では、１〜１０μｍの食品用濾過網を利用する。ここで、１０μｍを超える場合は、凝固されたゲルの形態が流出され、１μｍ未満の場合には、ほとんど濾過されなくなる（濾過網の過剰微細により）。 Then, in the filtration stage of the present invention, a 1 to 10 μm food filtration net is used. Here, if it exceeds 10 μm, the form of the coagulated gel flows out, and if it is less than 1 μm, it is hardly filtered (due to excessive fineness of the filtration net).
したがって、ゲル形態のエゴマ油を濾過するために前記のような数値に限定したものである。 Therefore, it is limited to the above-mentioned numerical values in order to filter the perilla oil in the gel form.
もし一般的な油圧フィルタを使用すれば、ゲル形態のエゴマ油の脂肪酸が圧力で濾過網上で溶けるので、本発明では、油圧フィルタを使用せずに、前述した１〜１０μｍの食品用濾過網を利用することになる。 If a general hydraulic filter is used, the fatty acids of the perilla oil in the gel form are dissolved on the filter net by pressure. Therefore, in the present invention, the above-mentioned 1 to 10 μm food filter net is used without using the hydraulic filter. Will be used.
前述した限定事項及び製造過程は、当業者が容易に考えることができない事項であり、反復的な実験を通じて確認した結果である。 The above-mentioned limitations and manufacturing process are matters that cannot be easily considered by those skilled in the art, and are the results confirmed through repeated experiments.
すなわち、本発明では、このような製造過程を経る理由及び限定された数値は、どのようにすれば搾油したエゴマ油を冷低温で凝固及び沈殿させ、冷低温から生成された凝固浮遊物を除去し、発癌性物質の発生を最小限に抑えることができるだろうかという動機から出発したので、従来エゴマ油の製造方法とは全く異なる製造方法である。 That is, in the present invention, the reason for undergoing such a manufacturing process and the limited numerical value are how to solidify and precipitate the squeezed perilla oil at low temperature and low temperature, and remove the solidified suspended matter generated from the cold and low temperature. However, since it started from the motive of whether the generation of carcinogenic substances could be minimized, it is a completely different production method from the conventional method for producing perilla oil.
一方、本発明の実施例１を介して製造されたエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物は、化粧品全体の重量に対して０．００１〜３０．０重量部（重量％）が含有されていることを特徴とする。 On the other hand, the cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 produced through Example 1 of the present invention is 0.001 to 30.0 parts by weight based on the total weight of the cosmetic product. It is characterized by containing (% by weight).
これにより、エゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物を含有した化粧品を製造することができるようになる。 This makes it possible to produce cosmetics containing a cosmetic composition containing perilla oil beauty oil and vegetable omega-3.
このとき、エゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の剤形は、化粧水、ジェル、水溶性リキッド、クリーム、エッセンス、水中油（Ｏ／Ｗ）型と油中水（Ｗ／Ｏ）型からなる基礎化粧料剤形及び；水中油型または油中水型のメイクアップベース、ファンデーション、スキンカバー、リップスティック、リップグロス、フェイスパウダー、ツーウェイケーキ、アイシャドウ、チークカラー、およびアイブロウペンシル類からなる色調化粧料剤形から選択されるいずれか一つであることを特徴とする。 At this time, the dosage form of the cosmetic composition containing egoma oil beauty oil and vegetable omega-3 is a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and in oil. Basic cosmetics form consisting of water (W / O) type and; make-up base of oil-in-water type or water-in-oil type, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, teak It is characterized in that it is any one selected from a color and a color-tone cosmetic formulation consisting of eyebrow pencils.
また、エゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物は、紫外線遮断用化粧品に含まれることができ、抗酸化用化粧品に含まれることができ、皮膚の保湿用化粧品に含まれることができ、皮膚の美白のための化粧品に含まれることができ、しわ、シミ及びそばかすの予防のための化粧品に含まれることができる。 In addition, cosmetic compositions containing freckle oil beauty oil and vegetable omega-3 can be included in UV-blocking cosmetics, can be included in antioxidant cosmetics, and can be used in skin moisturizing cosmetics. It can be included, it can be included in cosmetics for skin whitening, it can be included in cosmetics for the prevention of wrinkles, stains and freckles.
このとき、本発明の化粧料組成物は、化粧品全体の重量に対して０．００１〜３０．０重量部（重量％）が含有されていることを特徴とする。 At this time, the cosmetic composition of the present invention is characterized by containing 0.001 to 30.0 parts by weight (% by weight) with respect to the total weight of the cosmetic product.
例えば、本発明のエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物は、基本的に、皮膚に塗布されているので、当業界の化粧料組成物を参照して、通常製造されるいかなる剤型でも製造することができる。 For example, a cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 of the present invention is basically applied to the skin, so that the cosmetic composition of the art is usually referred to. Any dosage form produced can be produced.
例えば、溶液、懸濁液、乳濁液、ペースト、ゲル、クリーム、ローション、パウダー、石鹸、界面活性剤含有クレンジング、オイル、粉末状のファンデーション、乳濁液ファンデーション、ワックスファンデーション及びスプレーなどで剤形化することができるが、これに限定されるものではない。 For example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powdered foundations, emulsion foundations, wax foundations and sprays. However, it is not limited to this.
より詳細には、柔軟化粧水、栄養化粧水、栄養クリーム、マッサージクリーム、エッセンス、アイクリーム、クレンジングクリーム、クレンジングフォーム、クレンジングウォーター、マスクパック、スプレーまたはパウダーの剤形に製造することができる。 More specifically, it can be produced in the form of soft lotions, nourishing lotions, nourishing creams, massage creams, essences, eye creams, cleansing creams, cleansing foams, cleansing waters, mask packs, sprays or powders.
本発明の剤形がペースト、クリームまたはゲルである場合には、担体成分として動物性油、植物性油、ワックス、パラフィン、デンプン、トラカント、セルロース誘導体、ポリエチレングリコール、シリコン、ベントナイト、シリカ、タルク、酸化亜鉛などが含むことができる。 When the dosage form of the present invention is a paste, cream or gel, the carrier components include animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, etc. It can contain zinc oxide and the like.
本発明の剤形がパウダーまたはスプレーである場合には、担体成分としてラクトース、タルク、シリカ、アルミニウムヒドロキシド、カルシウムシリケート、ポリアミドパウダーなどが含まれることができ、特にスプレーである場合には、さらにクロロフルオロヒドロカーボン、プロパン／ブータン、ジメチルエーテルなどの推進体を含むことができる。 When the dosage form of the present invention is a powder or spray, the carrier component can include lactouse, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc., and further, especially when it is a spray. It can include propellants such as chlorofluorohydrocarbon, propane / butane, dimethyl ether and the like.
本発明の剤形が溶液または乳濁液である場合には、担体成分として、溶媒、溶解剤、油濁剤などが含まれることができ、具体的に水、エタノール、イソプロパノール、エチルカーボネート、エチルアセテート、ベンジルアルコール、ベンジルベンゾエート、プロピレングリコール、１，３−ブチルグリコールオイル、グリセロール脂肪族エステル、ポリエチレングリコール、ソルビタンの脂肪酸エステルなどが含まれることができる。 When the dosage form of the present invention is a solution or an emulsion, the carrier component may include a solvent, a solubilizer, an oil turbidant, and the like, and specifically, water, ethanol, isopropanol, ethyl carbonate, and ethyl. Acetates, benzyl alcohols, benzyl benzoates, propylene glycols, 1,3-butyl glycol oils, glycerol aliphatic esters, polyethylene glycols, fatty acid esters of sorbitan and the like can be included.
本発明の剤形が懸濁液である場合には、担体成分として、水、エタノール、プロピレングリコールなどの液状希釈剤；エトキシ化イソステアリルアルコール、ポリオキシエチレンソルビトールエステル、ポリオキシエチレンソルビタンエステルなどの懸濁剤；微小結晶性セルロース、アルミニウムメタヒドロキシド、ベントナイト、寒天またはトラガカントなどが含まれることができる。 When the dosage form of the present invention is a suspension, the carrier component is a liquid diluent such as water, ethanol, propylene glycol; ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, etc. Suspensions; microcrystalline cellulose, aluminum metahydroxyde, bentonite, agar or tragacant and the like can be included.
本発明の剤形が界面活性剤含有クレンジングである場合には、担体成分として脂肪族アルコール硫酸、脂肪族アルコールエーテル硫酸、スルホンコハク酸モノエステル、イセチオネート、イミダゾニウム誘導体、メチルタウレート、サルコシネート、脂肪酸アミドエーテル硫酸、アルキルアミドベタイン、脂肪族アルコール、脂肪酸グルリセリドゥ、脂肪酸ジエタノールアミド、植物性油、ラノリン誘導体、エトキシ化グリセロール脂肪酸エステルなどが含まれることができる。 When the dosage form of the present invention is a surfactant-containing cleansing, fatty alcohol sulfuric acid, fatty alcohol ether sulfuric acid, sulfone succinic acid monoester, isetionate, imidazolium derivative, methyl taurate, sarcosinate, fatty acid as carrier components. Amid ether sulfuric acid, alkylamide betaine, fatty alcohols, fatty acid gluliceridu, fatty acid diethanolamide, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters and the like can be included.
下記には、本発明で提示する効能実験を具体的に説明するようにする。本発明のエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物の製造方法によって製造されたエゴマ油化粧料組成物を用いて、人の皮膚細胞にＵＶ紫外線を照射及び誘導して皮膚の老化に対するエゴマ油の治療効果を評価したものである。 The efficacy experiments presented in the present invention will be specifically described below. Using the perilla oil cosmetic composition produced by the method for producing a perilla oil cosmetic composition containing the perilla oil beauty oil and vegetable omega-3 of the present invention, human skin cells are irradiated and induced with UV ultraviolet rays. This is an evaluation of the therapeutic effect of perilla oil on skin aging.
大韓民国の代表の油脂作物の一つであるエゴマは、シソ科に属する一年生の植物であって、大韓民国を含む東南アジアが原産であり、昔から単純な食用だけでなく、健康食品、高血圧の予防などの食品として多様に利用してきた。 Perilla frutescens, one of the representative oil and fat crops of the Republic of Korea, is an annual plant belonging to the Labiatae family and is native to Southeast Asia including the Republic of Korea. It has long been not only a simple food, but also a health food and prevention of high blood pressure. It has been used in various ways as a food product.
大韓民国でのエゴマの利用は、統一新羅時代にゴマと一緒に栽培した記録があり、全国の麓や道端の湿地で簡単に育ち、全国どこでも分布している。 The use of perilla in the Republic of Korea has a record of being cultivated with sesame during the Unified Silla era, and it grows easily at the foot of the country and in wetlands on the roadside, and is distributed everywhere in the country.
エゴマの効能は昔から東医宝鑑によれば、エゴマは性質が暖かく、毒がなく、味は酸っぱく、気を降りてくれるならない。 According to Dongui Bogam, the efficacy of perilla is warm, non-poisonous, sour in taste, and must be disheartening.
また、肝を豊かにし、その種は粥を沸かして食べると私たちの体を滑らかに美しく作ってくれて元気を回復させてくれる。「方薬合編」には、精髄を保護してくれて、渇きと咳嗽をなくし、体内の毒素を除去し、血液をきれいにしてくれる効能があり、他の多くの文献によれば、エゴマはリノレン酸とビタミンＥ、Ｆがたくさん入っているので、女性の健康と美肌に絶対に必要なものであり、肌が粗くそばかすやシミが多くの人と、日焼け後なかなか回復がない人に良いと紹介されている。 It also enriches the liver, and when the seeds are boiled and eaten, it makes our body smooth and beautiful and restores energy. "Pharmaceutical combination" has the effect of protecting the essence, eliminating thirst and coughing, removing toxins in the body, and cleaning the blood. According to many other documents, freckles Contains a lot of linolenic acid and vitamins E and F, so it is absolutely necessary for women's health and beautiful skin, and it is good for people with rough skin and many freckles and age spots, and those who do not recover easily after sunburn. Is introduced.
しかしながら、今までエゴマの効能中、皮膚効果のための科学的な研究が全く行われていないのが実情であり、これを基に製造されたエゴマ油とエゴマ油ビューティー化粧品がなく、効能評価実験を本発明で実施することになった。 However, the fact is that no scientific research has been conducted on the effects of perilla among the effects of perilla, and there are no perilla oil and perilla oil beauty cosmetics manufactured based on this, and efficacy evaluation experiments have been conducted. Was to be carried out in the present invention.
本実験は、人の皮膚細胞からエゴマ油がＵＶ照射で誘発された皮膚の老化を治療する有効性を評価して作用機序を分析しようとした。 This experiment attempted to evaluate the efficacy of perilla oil from human skin cells in treating UV-irradiated skin aging and analyze its mechanism of action.
実験方法としてエゴマ油の有効性を評価するために使用された人の皮膚細胞（Ｈｕｍａｎ ｄｅｒｍａｌ ｆｉｂｒｏｂｌａｓｔ）であるｎＨＤＦ細胞株は、１０％ ｆｅｔａｌ ｂｏｖｉｎｅ ｓｅｒｕｍ（ＦＢＳ、Ｇｉｂｃｏ、Ｃａｒｌｓｂａｄ、ＣＡ、ＵＳＡ）、１％ Ｌ−ｇｌｕｔａｍｉｎｅ、ｐｅｎｉｃｉｌｌｉｎ及びｓｔｒｅｐｔｏｍｙｃｉｎ（Ｃａｍｂｒｅｘ）を含有したＤｕｌｂｅｃｃｏ Ｍｏｄｉｆｉｅｄ Ｅａｇｌｅ Ｍｅｄｉｕｍ（ＤＭＥＭ、Ｗｅｌｇｅｎｅ）に３７℃、５％、ＣＯ２の条件のｉｎｃｕｂａｔｏｒで培養した。 The nHDF cell line, which is a human skin cell (Human dermal fibroblast) used to evaluate the effectiveness of egoma oil as an experimental method, is 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA), 1 The cells were cultured in Dulvecco Modified Eagle's Medium (DMEM, Welgene) containing% L-glutamine, penicillin and streptomycin (Cambrex) at 37 ° C., 5%, CO 2 incubator.
人の皮膚細胞のエゴマ油の細胞毒性を評価するために、ｎＨＤＦ細胞株を、９６ ｗｅｌｌ ｐｌａｔｅに３×１０４ｃｅｌｌｓ／ｗｅｌｌほど分注し、２４時間の間培養器で培養した。Ｎｏ処理群、五倍子処理群、エゴマ油処理群（０．５、１、１．５、２、２．５、３％）に分類して、各処理群は、様々な濃度で処理した後、２４時間経過後に培地を除去し、新しい培地２００ｕｌに乗り換えた後、５０μｌのＭＴＴ溶液（２ｍｇ／ｍｌｉｎＰＢＳ）を分注した後、ＣＯ２培養器で２４時間培養する。 To assess the cytotoxicity of perilla oil in human skin cells, nHDF cell lines were dispensed into 96-well plates at about 3 × 10 4 cells / well and cultured in incubators for 24 hours. Classified into No-treated group, quintuple-treated group, and perilla oil-treated group (0.5, 1, 1.5, 2, 2.5, 3%), and each treated group was treated with various concentrations and then treated. After 24 hours, the medium is removed, the medium is replaced with 200 ul of new medium, 50 μl of MTT solution (2 mg / mlin PBS) is dispensed, and the cells are cultured in a CO 2 incubator for 24 hours.
バッジを削除した後ｆｏｍａｚａｎを分解するために、１５０μｌのＤＭＳＯ（ｄｉｍｅｔｈｙｌ ｓｕｌｆｏｘｉｄｅ）を添加した後、１０分間２３０ｒｐｍで振盪培養し、５７０ｎｍでＭｏｌｅｃｕｌａｒ Ｄｅｖｉｃｅｓ ＶＥＲＳＡ ｍａｘ ｐｌａｔｅ ｒｅａｄｅｒ（Ｓｕｎｎｙｖａｌｅ、ＣＡ、ＵＳＡ）を用いて吸光度を測定した。 After removing the badge, 150 μl of DMSO (dimethyl sulfoxide) was added to decompose the fomasan, followed by shaking culture at 230 rpm for 10 minutes, and using a Molecular Devices VERSA max plate reader (Sunnyvale, CA, USA) at 570 nm. The absorbance was measured.
その結果、細胞の生存率は、様々な濃度のエゴマ油処理群で有意な変化を誘導しなかった（図７参照）。 As a result, cell viability did not induce significant changes in the perilla oil-treated groups at various concentrations (see FIG. 7).
このような結果は、エゴマ油が人の皮膚細胞に有意な毒性がないことを示している。 These results indicate that perilla oil is not significantly toxic to human skin cells.
また、肌の細胞株で老化を誘導する適切なＵＶ濃度を設定するために、ｎＨＤＦ細胞株を、９６ ｗｅｌｌ ｐｌａｔｅに３×１０４ｃｅｌｌｓ／ｗｅｌｌほど分注し、２４時間の間培養器で培養した。 In addition, in order to set an appropriate UV concentration that induces aging in the skin cell line, the nHDF cell line was dispensed into a 96-well plate by about 3 × 10 4 cells / well and cultured in an incubator for 24 hours. did.
ＵＶランプと細胞との間の距離が１３ｃｍになるように維持しながら、細胞の２５、５０、１００、２００、４００ｍＪまでの５つの部分を照射し、２４時間経過後ＭＴＴ ａｓｓａｙを介して細胞生存率を確認した。 Irradiate 5 parts of cells up to 25, 50, 100, 200, 400 mJ while maintaining the distance between the UV lamp and the cells at 13 cm, and after 24 hours, cell survival via MTT assay. I confirmed the rate.
ＵＶ−Ｂ照射装置は３６５／３００ｎｍの波長のＵＶ−Ｂを発光するＨａｎｄｈｅｌｄ ＵＶ ｌａｍｐ（Ｕｌｔｒａ−Ｌｕｍ、Ｃｌａｒｅｍｏｎｔ、ＣＡ）を使用し、照射量はＵＶ ｘ Ｒａｄｉｏｍｅｔｅｒ（ＵＶＰ、Ｕｐｌａｎｄ、ＣＡ、ＵＳＡ）を用いて測定した。 The UV-B irradiation device uses a Handheld UV lamp (Ultra-Lum, Claremont, CA) that emits UV-B having a wavelength of 365/300 nm, and the irradiation amount is UV x Radiometer (UVP, Upland, CA, USA). Measured using.
その結果、２５、５０、１００、２００、４００ｍＪで８５、７０、６０、５６、５２％の細胞生存率を見せ、適正ＵＶ ｄｏｓｅを７０％の細胞生存率を示した５０ｍＪに設定した（図８参照）。 As a result, the cell viability of 85, 70, 60, 56, 52% was shown at 25, 50, 100, 200, 400 mJ, and the appropriate UV dose was set to 50 mJ, which showed a cell viability of 70% (FIG. 8). reference).
皮膚細胞でエゴマ油がＵＶ照射によって誘発された細胞死滅を抑制する効能を評価するために、ｎＨＤＦ細胞株を、９ ６ｗｅｌｌ ｐｌａｔｅに３×１０４ｃｅｌｌｓ／ｗｅｌｌほど分注し、２４時間の間培養器で培養した。 To evaluate the efficacy of perilla oil in suppressing UV irradiation-induced cell death in skin cells, nHDF cell lines were dispensed into 96-well plates at about 3 × 10 4 cells / well and cultured for 24 hours. It was cultured in a vessel.
Ｎｏ処理群を除いた残りの群に５０ｍＪのＵＶを照射した後Ｎｏ処理群、五倍子処理群、エゴマ油処理群（０．６２５、１．２５、２．５％）に分類して試料を処理した後、２４時間経過し後ろＭＴＴ ａｓｓａｙを実施した。 After irradiating the remaining group excluding the No treatment group with 50 mJ of UV, the samples were treated by classifying them into the No treatment group, the quintuplet treatment group, and the perilla oil treatment group (0.625, 1.25, 2.5%). After that, 24 hours later, a posterior MTT assy was performed.
その結果、ＵＶ照射は皮膚細胞の死滅を誘導したが、エゴマ油を処理した群で細胞生存率が１２％、１６％、１９％増加した（図９ 参照）。 As a result, UV irradiation induced the death of skin cells, but the cell viability increased by 12%, 16%, and 19% in the group treated with perilla oil (see FIG. 9).
このような結果は、エゴマ油がＵＶ照射によって誘発された細胞死滅を有意に抑制することを提示しており、紫外線遮断の効果および皮膚の保護効果が大きいことが分かる。 Such results suggest that perilla oil significantly suppresses UV irradiation-induced cell death, and it can be seen that the effect of blocking ultraviolet rays and the effect of protecting the skin are great.
また、皮膚の細胞からエゴマ油がＵＶ照射によって誘発された活性酸素種の生成を抑制する効能を評価するために、ＵＶ照射されたｎＨＤＦ細胞株でエゴマ油を処理した後、活性酸素種の生成を分析した。 In addition, in order to evaluate the efficacy of perilla oil from skin cells to suppress the production of reactive oxygen species induced by UV irradiation, after treating perilla oil with a UV-irradiated nHDF cell line, the production of reactive oxygen species Was analyzed.
まず、ｎＨＤＦ細胞株を、９６ ｗｅｌ ｌｐｌａｔｅに３×１０４ｃｅｌｌｓ／ｗｅｌｌほど分注し、２４時間の間培養器で培養した。 First, the nHDF cell line was dispensed into 96-well llate in an amount of about 3 × 10 4 cells / well and cultured in an incubator for 24 hours.
Ｎｏ処理群を除いた残りの群に５０ｍＪのＵＶを照射した後、Ｎｏ処理群、五倍子処理群、エゴマ油処理群（０．６２５、１．２５、２．５％）に分類して試料を処理した。 After irradiating the remaining group excluding the No treatment group with 50 mJ of UV, the samples were classified into the No treatment group, the quintuplet treatment group, and the perilla oil treatment group (0.625, 1.25, 2.5%). Processed.
２４時間経過後に培地を除去し、１００μＭの２′、７′−ｄｉｃｈｌｏｒｏｆｌｕｏｒｅｓｃｅｉｎ ｄｉａｃｅｔａｔｅ（ＤＣＦＨ−ＤＡ）（Ｓｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．）分注した後、ＣＯ２インキュベーターで３０分間培養した。溶液を除去し、ＰＢＳで３回洗浄した後、ｆｌｕｏｒｅｓｃｅｎｔ ｍｉｃｒｏｓｃｏｐｅ（Ｅｃｌｉｐｓｅ ＴＸ１００、Ｎｉｋｏｎ、Ｔｏｋｙｏ、Ｊａｐａｎ）で観察した。 After 24 hours, the medium was removed, 100 μM of 2', 7'-dichlorofluorescein diacenate (DCFH-DA) (Sigma-Aldrich Co.) was dispensed, and the cells were cultured in a CO 2 incubator for 30 minutes. The solution was removed, washed 3 times with PBS, and then observed with a fluorescence microscope (Eclipse TX100, Nikon, Tokyo, Japan).
その結果、ＵＶ照射群でＲＯＳが増加することを示し、エゴマ油を処理した群では、全体的にＲＯＳが減少する傾向を見せ、高濃度のグループで有意に減少した（図１０参照）。 As a result, it was shown that ROS increased in the UV-irradiated group, the ROS tended to decrease overall in the group treated with perilla oil, and significantly decreased in the high-concentration group (see FIG. 10).
このような結果は、エゴマ油がＵＶ照射によって誘導される活性酸素種の生成を抑制する効能があることを提示しており、シミやそばかすの予防になって美白効果があることが分かる。 Such a result suggests that perilla oil has an effect of suppressing the production of active oxygen species induced by UV irradiation, and it can be seen that it has a whitening effect by preventing spots and freckles.
また、皮膚の細胞からエゴマ油がＵＶ照射によって誘発された抗酸化酵素活性の回復に及ぼす影響を評価するために、ＳＯＤ活性を分析した。 SOD activity was also analyzed to assess the effect of perilla oil on the recovery of UV irradiation-induced antioxidant enzyme activity from skin cells.
皮膚細胞でＳＯＤ酵素活性は、ＳＯＤ ａｓｓａｙ Ｋｉｔ−ＷＳＴ（Ｄｏｊｉｎｄｏ Ｍｏｌｅｃｕｌａｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ、Ｒｏｃｋｖｉｌｌｅ、ＭＤ）を用いて、企業の推奨法に基づいて測定した。 SOD enzyme activity in skin cells was measured using SOD assay Kit-WST (Dojindo Molecular Technologies, Rockville, MD) according to company recommendations.
ｎＨＤＦ細胞株を、９６ ｗｅｌｌ ｐｌａｔｅに３×１０４ ｃｅｌｌｓ／ｗｅｌｌほど分注し、２４時間の間培養器で培養した。 The nHDF cell line was dispensed into a 96-well plate at about 3 × 10 4 cells / well and cultured in an incubator for 24 hours.
Ｎｏ処理群を除いた残りの群に５０ｍＪのＵＶを照射した後、Ｎｏ処理群、五倍子処理群、エゴマ油処理群（０．６２５、１．２５、２．５％）に分類して試料を処理した。 After irradiating the remaining group excluding the No treatment group with 50 mJ of UV, the samples were classified into the No treatment group, the quintuplet treatment group, and the perilla oil treatment group (0.625, 1.25, 2.5%). Processed.
２４時間経過後に、細胞をｆｒｅｅｚｉｎｇ−ｔｈａｗｉｎｇ方法で均質化し、遠心分離して上層液を収穫した。 After 24 hours, the cells were homogenized by the freezing-thwing method and centrifuged to harvest the upper layer fluid.
細胞均質液を９６ ｗｅｌｌプレートに入れ、ＷＳＴ−１（２−（４−ｉｏｄｏｐｈｅｎｙｌ）−３−（４−ｎｉｔｒｏｐｈｅｎｙｌ）−５−２，４−ｄｉｓｕｌｆｏｐｈｅｎｙｌ）−２Ｈ−ｔｅｔｒａｚｏｌｉｕｍ、ｍｏｎｏ−ｓｏｄｉｕｍ ｓａｌｔ）とｘａｎｔｈｉｎｅ ｏｘｉｄａｓｅ ｗｏｒｋｉｎｇ ｓｏｌｕｔｉｏｎを添加した後、３７℃で２０分間培養した後、Ｓｏｆｔ Ｍａｘ Ｐｒｏ ５ ｓｐｅｃｔｒｏｐｈｏｔｏｍｅｔｅｒ（Ｍｏｌｅｃｕｌａｒ Ｄｅｖｉｃｅｓ、Ｓｕｎｎｙｖａｌｅ、ＣＡ、ＵＳＡ）を用いて４５０ｎｍで測定した。 Put the cell homogenic solution in a 96-well plate, and put WST-1 (2- (4-iodopheneyl) -3- (4-nitrophenol) -5-2,4-disulphophenyl) -2H-tetrazolium, mono-sodium salt) and xanthine. After adding an oxidase working solution, the cells were cultured at 37 ° C. for 20 minutes, and then measured at 450 nm using Soft Max Pro 5 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA).
その結果、ＳＯＤ活性は、ＵＶを照射した後、急激に増加したが、エゴマ油を処理した群では４９％、５１％、２６％に減少した（図１１参照）。 As a result, the SOD activity increased sharply after UV irradiation, but decreased to 49%, 51%, and 26% in the group treated with perilla oil (see FIG. 11).
このような結果は、エゴマ油が抗酸化酵素の活性を阻害する効能があることを示している。 Such results indicate that perilla oil has the effect of inhibiting the activity of antioxidant enzymes.
一般的に、コラーゲンは、皮膚の弾力性と回復を誘導する重要な成分でＭＭＰのようなコラーゲン分解酵素によって分解される。 In general, collagen is an important component that induces skin elasticity and recovery and is degraded by collagen-degrading enzymes such as MMPs.
このような酵素は、ＵＶ照射でによって増加する。 Such enzymes are increased by UV irradiation.
皮膚細胞でエゴマ油がＵＶ照射によって誘発された抗コラーゲン分解酵素の発現を抑制する効能を評価するために、人の皮膚細胞からＭＭＰ１タンパク質の発現をｗｅｓｔｅｒｎ ｂｌｏｔを用いて確認した。 In order to evaluate the effect of perilla oil on suppressing the expression of UV-induced anti-collagen degrading enzyme in skin cells, the expression of MMP1 protein from human skin cells was confirmed using Western blotting.
ｎＨＤＦ細胞株を１．５×１０５ ｃｅｌｌｓ／ｗｅｌｌほど分注し、２４時間の間培養器で培養した。 The nHDF cell line was dispensed at about 1.5 × 10 5 cells / well and cultured in an incubator for 24 hours.
Ｎｏ処理群を除いた残りの群に５０ｍＪのＵＶを照射した後、Ｎｏ処理群、五倍子処理群、エゴマ油処理群（０．６２５、１．２５、２．５％）に分類して試料を処理した。 After irradiating the remaining group excluding the No treatment group with 50 mJ of UV, the samples were classified into the No treatment group, the quintuplet treatment group, and the perilla oil treatment group (0.625, 1.25, 2.5%). Processed.
２４時間経過後にＰｒｏｔｅｉｎ Ｅｘｔｒａｃｔｉｏｎ Ｓｏｌｕｔｉｏｎ（ＩＮｔＲＯＮ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ、Ｓｅｏｎｇｎａｍ、Ｋｏｒｅａ）を利用して粉砕した後、１３，０００ｒｐｍで５分間遠心分離してタンパク質を分離し、ＳＭＡＲＴＴＭ ＢＣＡ Ｐｒｏｔｅｉｎ Ａｓｓａｙ Ｋｉｔ（ｉＮｔＲＯＮ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ）を用いてタンパク質を定量してｗｅｓｔｅｒｎ ｂｌｏｔで使用した。 After 24 hours, the protein was pulverized using Protein Assay Solution (INTRON Biotechnology, Seongnam, Korea), and then centrifuged at 13,000 rpm for 5 minutes to separate the protein, and SMART TM BCA Protein Assay Kit (iN) was used. The protein was quantified and used in Western blot.
１０％ＳＤＳ−ＰＡＧＥ ｇｅｌに３０μｇのタンパク質を電気泳動した後、ＥＣＬ ｍｅｍｂｒａｎｅ（ＲＰＮ２０２０Ｄ、Ａｍｅｒｓｈａｍ Ｌｉｆｅ Ｓｃｉｅｎｃｅ、Ｐｉｓｃａｔａｗａｙ、ＮＪ、ＵＳＡ）に転移して、３％ ｓｋｉｍ ｍｉｌｋで１時間ブロッキングした。 After electrophoresis of 30 μg of protein on 10% SDS-PAGE gel, it was transferred to ECL membrane (RPN2020D, Amersham Life Science, Piscataway, NJ, USA) and blocked on 3% skim milk for 1 hour.
各ｍｅｍｂｒａｎｅは、次のようなａｎｔｉ−ＭＭＰ−１／８ ａｎｔｉｂｏｄｙ（Ｈ−１２９、ＳａｎｔａＣｒｕｚ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ）、ａｎｔｉ−β−ａｃｔｉｎ ａｎｔｉｂｏｄｙ（Ｓｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．）に４℃で一晩培養した後、ＨＲＰ−ｃｏｎｊｕｇａｔｅｄされたｓｅｃｏｎｄａｒｙ ａｎｔｉｂｏｄｙを添加してＥＣＬ Ｋｉｔ（Ａｍｅｒｓｈａｍ Ｌｉｆｅ Ｓｃｉｅｎｃｅ）を用いてｄｅｖｅｌｏｐした。 Each membrane was cultured overnight at 4 ° C. in the following anti-MMP-1 / 8 antibody (H-129, Santa Cruz Biotechnology) and anti-β-actin antibody (Sigma-Aldrich Co.), and then HRP-. Conjugated secondary antibody was added and developed using ECL Kit (Amersham Life Science).
その結果、ＭＭＰ−１の発現は、Ｎｏ処理群に比べてＵＶ照射群で発現が増加したが、エゴマ油を濃度別に処理した群では１２％、１７％、２４％に減少した（図１２参照）。 As a result, the expression of MMP-1 increased in the UV-irradiated group as compared with the No-treated group, but decreased to 12%, 17%, and 24% in the group treated with perilla oil by concentration (see FIG. 12). ).
このような結果は、エゴマ油が肌の老化を促進するコラーゲン分解酵素の発現を抑制することを示している。 These results indicate that perilla oil suppresses the expression of collagen-degrading enzymes that accelerate skin aging.
この結果として、皮膚のしわの予防と角質除去効果があることがわかる。 As a result, it can be seen that it has the effect of preventing wrinkles on the skin and removing dead skin cells.
前記のような効能実験結果を要約すれば、エゴマ油が人の皮膚細胞からＵＶ照射によって誘発された細胞の死滅、活性酸素種の生成、ＳＯＤ活性、コラーゲン分解酵素の発現を効果的に抑制することにより、エゴマ油の抗酸化効果と皮膚の保湿、美白としわやシミやそばかすを防ぐことがわかり、本発明を使用して製造されたエゴマ油ビューティーオイル及び植物性オメガ−３が含有された化粧料組成物を化粧品に配合させると、本発明で提示した人体の皮膚に有益であり、紫外線遮断効果と抗酸化効能と皮膚の保湿、美白としわ、シミ及びそばかすの予防が可能になる。 To summarize the results of the above efficacy experiments, egoma oil effectively suppresses UV irradiation-induced cell death, active oxygen species production, SOD activity, and collagen degrading enzyme expression from human skin cells. As a result, it was found that the antioxidant effect of ultraviolet oil, moisturizing the skin, whitening and preventing wrinkles, stains and freckles were found, and the ultraviolet oil beauty oil and vegetable omega-3 produced using the present invention were contained. Incorporating the cosmetic composition into cosmetics is beneficial to the human skin presented in the present invention, and enables ultraviolet blocking effect, antioxidant effect, skin moisturizing effect, whitening and wrinkles, stains and freckles.
前記は本発明を実施例によって詳細に説明したが、本発明は実施例によって限定されず、本発明が属する技術分野において通常の知識を有するものであれば本発明の思想と精神を離れることなく、本発明を修正または変更できるであろう。 Although the present invention has been described in detail by way of examples, the present invention is not limited to the above examples, and any person who has ordinary knowledge in the technical field to which the present invention belongs does not depart from the idea and spirit of the present invention. , The present invention could be modified or modified.
本発明の範囲は、前記の詳細な説明ではなく、後述する特許請求の範囲によって示され、特許請求の範囲の意味及び範囲そしてその等価概念から導き出されるすべての変更または変形された形態が本発明の範囲に含まれるものと解釈されるべきでする。 The scope of the present invention is shown not by the above detailed description but by the scope of claims described later, and all modified or modified forms derived from the meaning and scope of claims and their equivalent concepts are the present invention. Should be construed as being included in the scope of.
Ｓ１００ エゴマの洗浄段階
Ｓ２００ 乾燥段階
Ｓ３００ 搾油段階
Ｓ４００ 沈殿段階
Ｓ５００ 冷低温処理段階
Ｓ６００ 濾過段階
S100 Perilla washing stage S200 Drying stage S300 Oil extraction stage S400 Precipitation stage S500 Cold and low temperature treatment stage S600 Filtration stage
Claims (10)
エゴマを洗浄器に投入して、５〜２０分間洗浄するエゴマの洗浄段階S１００と；
前記エゴマの洗浄段階S１００を介して洗浄されたエゴマを水分率が４〜７％になるように、１５〜８０℃の冷温風で１〜５時間乾燥させる乾燥段階S２００と；
記乾燥段階S２００を介して乾燥されたエゴマを搾油器に投入し、０〜４９℃の温度で圧力を加えて、５〜２０分間エゴマ油を搾油する搾油段階S３００と；
前記搾油段階S３００を介して搾油されたエゴマ油を沈殿タンクに投入し、１０分以上、自然沈殿させる沈殿段階S４００と；
前記沈殿段階S４００を介して沈殿させたエゴマ油が８℃以下になるように冷低温処理する冷低温処理段階S５００と；
前記冷低温処理段階S５００を介して冷低温処理されたエゴマ油を１〜１０μｍの食品用濾過網を利用して濾過させる濾過段階S６００と;を含んで構成されるが、
前記濾過段階S６００は、作業場の温度が１０℃以下になるように温度を設定した後に遂行されることによって、エゴマ油に含まれている様々な成分の各々が融点以下状態で濾過することにより、冷低温から生成された凝固浮遊物を除去し、植物性オメガ-３であるリノレン酸含有を向上させることを特徴とするエゴマ油の紫外線遮断効果、抗酸化効能、皮膚の保湿と美白、及びしわ、シミ及びそばかすの予防が可能なエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物の製造方法。 In the method for producing a cosmetic composition containing perilla oil beauty oil and vegetable omega-3, which can block the ultraviolet rays of perilla oil, have an antioxidant effect, moisturize and whiten the skin, and prevent wrinkles, stains and freckles. ,
With the perilla cleaning step S100, the perilla is placed in a washer and washed for 5 to 20 minutes;
With the drying step S200, in which the perilla washed through the washing step S100 of the perilla is dried with cold and warm air at 15 to 80 ° C. for 1 to 5 hours so that the moisture content becomes 4 to 7%;
Note: The perilla oil dried through the drying step S200 is put into an oil press, and pressure is applied at a temperature of 0 to 49 ° C. to squeeze the perilla oil for 5 to 20 minutes.
The perilla oil squeezed through the oil extraction step S300 is put into a settling tank and naturally settled for 10 minutes or more with the settling step S400;
With the cold / low temperature treatment step S500, in which the perilla oil precipitated through the precipitation step S400 is cold / low temperature treated so as to be 8 ° C. or lower;
It is composed of a filtration step S600 and; in which the perilla oil that has been subjected to the cold / low temperature treatment step S500 is filtered using a 1 to 10 μm food filtration net.
The filtration step S600 is carried out after setting the temperature so that the temperature of the workplace is 10 ° C. or lower, so that each of the various components contained in the perilla oil is filtered in a state of being below the melting point. Perilla oil, which is characterized by removing coagulated suspended matter generated from cold and low temperatures and improving the content of linolenic acid, which is a vegetable omega-3, has an ultraviolet blocking effect, an antioxidant effect, moisturizing and whitening of the skin, and wrinkles. A method for producing a cosmetic composition containing perilla oil beauty oil and vegetable omega-3 capable of preventing stains and freckles.
化粧品１００重量％に対して０.００１〜３０.０重量％が含有されていることを特徴とするエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 produced by the production method according to claim 1 is
A cosmetic composition containing perilla oil beauty oil and vegetable omega-3, which is characterized by containing 0.001 to 30.0% by weight based on 100% by weight of cosmetics.
化粧水、ジェル、水溶性リキッド、クリーム、エッセンス、水中油(O/W)型と油中水(W/O)型からなる基礎化粧料剤形及び;水中油型または油中水型のメイクアップベース、ファンデーション、スキンカバー、リップスティック、リップグロス、フェイスパウダー、ツーウェイケーキ、アイシャドウ、チークカラー、およびアイブロウペンシル類からなる色調化粧料剤形から選択されるいずれか一つであることを特徴とするエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The dosage form of the cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 produced by the production method according to claim 1 is
Basic cosmetics consisting of lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type and; oil-in-water type or water-in-oil type makeup Characterized by being one of the color cosmetic formulations selected from up-base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, teak color, and eyebrow pencils. A cosmetic composition containing Egoma oil beauty oil and vegetable omega-3.
紫外線遮断用であることを特徴とする請求項２に記載のエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 is
The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 according to claim 2, which is for blocking ultraviolet rays.
抗酸化用であることを特徴とする請求項２に記載のエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 is
The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 according to claim 2, which is for antioxidant use.
皮膚の保湿用であることを特徴とする請求項２に記載のエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 is
The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 according to claim 2, which is for moisturizing the skin.
皮膚の美白用であることを特徴とする請求項２に記載のエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 is
The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 according to claim 2, which is for whitening the skin.
シワ、シミ及びそばかすの予防用であることを特徴とする請求項２に記載のエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 The cosmetic composition containing the perilla oil beauty oil and the vegetable omega-3 is
The cosmetic composition containing the perilla oil beauty oil and vegetable omega-3 according to claim 2, which is for preventing wrinkles, blemishes and freckles.
エゴマ油を冷低温で凝固及び沈殿させて、冷低温で生成されている凝固浮遊物を除去した植物性オメガ-３であるリノレン酸を含有することを特徴とし、
化粧品１００重量％に対して０.００１〜３０.０重量％が含有されていることを特徴とするエゴマ油ビューティーオイル及び植物性オメガ-３が含有された化粧料組成物。 In a cosmetic composition containing perilla oil beauty oil and vegetable omega-3, which can block the ultraviolet rays of perilla oil, have an antioxidant effect, moisturize and whiten the skin, and prevent wrinkles, stains and freckles.
It is characterized by containing linolenic acid, which is a vegetable omega-3, obtained by coagulating and precipitating perilla oil at low temperature and low temperature to remove coagulated suspended matter generated at low temperature.
A cosmetic composition containing perilla oil beauty oil and vegetable omega-3, which is characterized by containing 0.001 to 30.0% by weight based on 100% by weight of cosmetics.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102459946B1 (en) | 2020-08-11 | 2022-10-28 | 진바이오셀 주식회사 | Composition for preventing skin aging comprising perilla seed oil ethanol extract, Anemone Rivularis or Cyperi Rhizoma |
| KR102496423B1 (en) | 2020-08-11 | 2023-02-06 | 진바이오셀 주식회사 | Composition for preventing skin aging comprising perilla seed oil ethanol extract |
| KR20230011167A (en) * | 2021-07-13 | 2023-01-20 | 임수복 | composition for preventing or treating retinal epithelial cell disease and manufacturing method of it |
| KR102371272B1 (en) | 2021-08-05 | 2022-03-07 | 진바이오셀 주식회사 | Composition for improving skin trouble comprising of perilla seed oil and cyperi rhizoma extract |
| KR20230143017A (en) | 2022-04-04 | 2023-10-11 | 주식회사 흑설란 | Functional cosmetic composition and its production |
| KR102469550B1 (en) | 2022-09-22 | 2022-11-22 | 주식회사 올바른 | Cosmetic composition with uv protection, whitening and wrinkle improvement effects |
| KR102639495B1 (en) | 2023-11-28 | 2024-02-22 | 주식회사 부쉬맨 | Waterproof sunscreen composition |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20180011552A (en) * | 2016-07-25 | 2018-02-02 | 현대모비스 주식회사 | Apparatus and method for generating around view |
| KR20180068359A (en) * | 2016-12-13 | 2018-06-22 | 원광대학교산학협력단 | A cosmetic composition manufactured by using raw perilla seed oil, and the cosmetic manufactured by the composition |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3170700B2 (en) * | 1992-11-12 | 2001-05-28 | 株式会社コーセー | Cosmetic for preventing skin aging |
| KR101370668B1 (en) * | 2012-04-04 | 2014-03-04 | 정정은 | Composition for health foods for memory enhancement and learning capabilities |
| KR20150027327A (en) * | 2013-08-29 | 2015-03-12 | 영농조합법인 대평 | Gel-type mask pack compounds containing perilla seed, gel-type maskpack prepared by using the same, and method for manufacturing the gel-type maskpack |
| KR20170053407A (en) | 2015-11-06 | 2017-05-16 | 부산대학교 산학협력단 | Composition containing alpha linoleic acid isolated from perilla seed oil |
| KR101828406B1 (en) * | 2016-07-25 | 2018-02-13 | 임수복 | Perilla oil and Method for manufacturing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180011552A (en) * | 2016-07-25 | 2018-02-02 | 현대모비스 주식회사 | Apparatus and method for generating around view |
| KR20180068359A (en) * | 2016-12-13 | 2018-06-22 | 원광대학교산학협력단 | A cosmetic composition manufactured by using raw perilla seed oil, and the cosmetic manufactured by the composition |
Non-Patent Citations (1)
| Title |
|---|
| 安部隆: "必須脂肪酸と健康", フレグランス ジャーナル, vol. 43, no. 11, JPN6021006936, 15 November 2015 (2015-11-15), pages 94 - 95, ISSN: 0004624604 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114504523A (en) * | 2022-01-05 | 2022-05-17 | 郑重远 | A compound liquid containing Perilla seed oil and oleum Rosae Rugosae for caring skin |
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