JP2010111602A - Antioxidant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant which inhibits the generation of active oxygen and radicals in vivo, inhibits the production of lipid peroxides, and can thereby prevent and treat aging of skins such as deterioration in the elasticity of the skins, the inflammation of the skins, and the pigmentation of the skins. <P>SOLUTION: There is provided the antioxidant characterized by containing an extract of one or more plants selected from Iris ensata var. ensata, Iris sanguinea Hornem., and Iris laevigata Fisch. as an active ingredient. The antioxidant has an active oxygen-scavenging action and/or a radical-scavenging action, and can be used as a radical scavenger, an active oxygen-scavenger. Also, there is provided a cosmetic containing the antioxidant. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cosmetic containing an antioxidant, an active oxygen scavenger and a radical scavenger obtained from a natural plant, and an extract from the plant. More specifically, the skin active ingredient is an extract of one or more plants selected from Iris ensata var. Ensata, Iris sanguinea Hornem. And Iris laevigata Fisch. The present invention relates to a cosmetic that can renew an antioxidant function, eliminate active oxygen and / or radicals, prevent skin aging, and maintain a youthful skin state.

  As for the use of Iris genus plants in cosmetics, skin external preparations for skin whitening containing a plant extract of Iris genus (Ayame genus) (Patent Document 1), relaxation of skin / subcutaneous tissue containing Ayame extract Cosmetics (patent document 2) for treating ordinary wrinkles and small wrinkles for relieving, drugs intended for stimulating immune defenses including extracts of iridaceae plants (patent document 3), extracts of genus iris A female hormone production promoter (Patent Document 4) is disclosed. However, it is not known that the extract of the genus Iris, especially the flower of the iris, Iris, oyster butterfly, exhibits remarkable antioxidant action, active oxygen scavenging action and radical scavenging action.

In recent years, active oxygen has attracted attention as a factor that particularly oxidizes biological components, and its adverse effect on living organisms has become a problem. Active oxygen is thought to decompose, denature or crosslink biological tissues such as collagen, and to oxidize fats and oils to produce lipid peroxides that damage cells, and these disorders caused by active oxygen However, it is considered to cause aging such as skin wrinkle formation and skin elasticity reduction, inflammation, skin pigmentation, and the like (see Non-Patent Document 1).
Therefore, prevention and treatment of skin aging such as reduced skin elasticity, skin inflammation, skin inflammation, skin pigmentation, etc. through inhibition of generation of active oxygen and in vivo radicals, suppression of lipid peroxide production, etc. It is considered possible.
Japanese Patent Laid-Open No. 9-30954 Japanese Patent Laid-Open No. 10-7545 JP 2002-80383 A JP 2005-171098 A Pregrance Journal Extra Number No. 14156-161, 1995

  The present invention has been made in view of the above-described circumstances, and its purpose is to inhibit the generation of lipid peroxides by inhibiting the generation of active oxygen and in vivo radicals, thereby reducing the elasticity of the skin and the like. It is to provide a substance capable of preventing and treating skin aging, skin inflammation, skin pigmentation, and the like.

In order to achieve the above object, the antioxidant according to the first invention is selected from one kind selected from Japanese iris (Iris ensata var. Ensata), iris (Iris sanguinea Hornem.) And oyster (Iris laevigata Fisch.) It contains two or more kinds of plant extracts as active ingredients.
In the present invention, the extract preferably has an active oxygen scavenging action and / or a radical scavenging action.
Moreover, it is preferable that it is a radical scavenger which contains the said antioxidant as an active ingredient.
Moreover, it is preferable that it is an active oxygen scavenger which contains the said antioxidant as an active ingredient.
Moreover, it is preferable that it is cosmetics containing the said antioxidant.

  According to the present invention, by inhibiting the generation of active oxygen and in vivo radicals, the production of lipid peroxide is suppressed, and skin aging such as skin elasticity reduction, skin inflammation, skin pigmentation, etc. We can provide something that can be prevented and treated.

  Next, embodiments of the present invention will be described with reference to the drawings. However, the technical scope of the present invention is not limited by these embodiments, and various forms can be made without changing the gist of the invention. Can be implemented. Further, the technical scope of the present invention extends to an equivalent range.

Antioxidants, active oxygen scavengers and radical scavengers according to the present invention all contain an extract from hanashobu, iris, oyster butterfly as active ingredients.
Here, the "extract" from hanashobu, iris, oyster butterfly, the extract obtained from each plant by the extraction process, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, Any of these crudely purified products or purified products are included.
Hanashobu, iris, and oyster are all plants belonging to the genus Iridaceae, which are native and cultivated in Japan and are readily available.
Examples of the constituent parts of the flowering plant, iris, oyster butterfly used as the extraction raw material include a leaf part, a stem part, and a root part, and any part thereof can be used in the present invention.

  Details of the antioxidant, active oxygen scavenging action and / or radical scavenging action contained in extracts from honeybee, iris, oyster butterfly are unknown, but depending on the extraction method commonly used for plant extraction An extract having an active oxygen scavenging action and / or a radical scavenging action can be obtained from Japanese red pepper, iris, oyster grasshopper. For example, the raw material for extraction can be obtained by chopping or grinding it as it is and subjecting it to extraction with an extraction solvent. If necessary, it may be extracted after drying with sunlight or a dryer. In addition, hanashobu, iris, oyster butterfly may be used as an extraction raw material after pretreatment such as degreasing and removal of colored components such as chlorophyll with a nonpolar solvent such as hexane and benzene. By performing a pretreatment such as degreasing, extraction with polar solvents such as honeybee, iris, and oyster can be efficiently performed.

As the extraction solvent, it is preferable to use a polar solvent, and it is particularly preferable to use water or a hydrophilic organic solvent or a mixture thereof at room temperature, a low temperature, or a temperature not higher than the boiling point of the solvent.
Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, in the present invention, water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

Examples of hydrophilic organic solvents that can be used as the extraction solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.
When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed solution of water and a lower alcohol, 1 to 90 parts by weight of a lower alcohol with respect to 10 parts by weight of water, and when using a mixed solution of water and a lower aliphatic ketone, When using a mixed liquid of 1 to 40 parts by weight of a lower ketone and 10 parts by weight of water and a polyhydric alcohol, 1 to 90 parts by weight of a polyhydric alcohol is mixed with 10 parts by weight of water. preferable.

The extraction treatment is not particularly limited as long as it can elute the soluble components contained in Japanese iris, iris, and oyster butter into the extraction solvent, and can be performed according to a conventional method. In the extraction process, it is not necessary to adopt a special extraction method, and any apparatus can be used at room temperature, low temperature or reflux heating.
Specifically, the extraction raw material is put into a processing container filled with the extraction solvent, and after standing for 30 minutes to 96 hours to elute soluble components while stirring occasionally as necessary, filtration or centrifugation is performed. To remove the solid matter to obtain an extract. The amount of extraction solvent is usually 2 to 20 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 96 hours at 0 to 95 ° C. when water is used as the extraction solvent, and usually 0 to 80 when a mixed solvent of water and ethanol is used as the extraction solvent. It is about 30 minutes to 48 hours at ° C. Moreover, extraction efficiency can be raised by repeating the same operation.

When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a solid extract is obtained. However, the extract from hanashobu, iris, oyster butterfly does not have to be a solid extract, and may be in the state of the extract or its concentrate. Extracts from these flowers, iris, oyster butterfly are purified by various methods such as filtration, activated carbon treatment, adsorption resin treatment, ion exchange 4 resin, liquid-liquid countercurrent distribution, etc., as long as the physiological activity is not reduced. It may be used after that.
Extracts from Hanashobu, iris, oyster butterfly have no specific odor and are effective in small amounts, so they do not affect the color of cosmetics and are used as antioxidants, active oxygen scavengers or radical scavengers However, if necessary, it can be treated for the purpose of improving the activity, decolorizing and deodorizing, or can be mixed with an optional auxiliary agent and used.

The extract from honeybee, iris, oyster butterfly obtained as described above has an active oxygen scavenging action and / or a radical scavenging action, and using these actions, an antioxidant, an active oxygen scavenger, or a radical It can be used as an active ingredient of an erasing agent in skin cosmetics, hair cosmetics, quasi drugs and the like.
Here, the antioxidant action of the extract from hanashobu, iris, oyster butterfly is exhibited based on the active oxygen scavenging action and / or the radical scavenging action. “Active oxygen” includes superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like. The term “radical” means a molecule or atom having one or more unpaired electrons, and includes superoxide, hydroxy radical, DPPH and the like.

Extracts from Japanese iris, iris, oyster butterfly, and antioxidants, active oxygen scavengers and radical scavengers containing such extracts as active ingredients prevent and / or prevent skin aging, skin inflammation and skin pigmentation Or it can be improved, and since it is excellent in use feeling and safety when applied to the skin, it is suitable for blending into skin and hair cosmetics.
There are no particular limitations on the types of skin cosmetics that can be blended with the extract from hanashobu, iris, oyster butterfly. , Hair lotion, soap, body shampoo and the like.

The blending amount of the extract from hanashobu, iris, oyster butterfly in the skin cosmetic of the present invention can be adjusted as appropriate depending on the type of skin cosmetic, physiological activity of the extract, etc. In the case of a simple extract, it is about 0.001 to 10% by mass in terms of dry matter.
The skin cosmetic of the present invention is usually used for the production of skin cosmetics unless it interferes with the antioxidant, active oxygen scavenging action and / or radical scavenging action of the extract from hanashobu, iris, oyster butterfly Various main agents and auxiliaries, and other optional auxiliaries can be used. The skin cosmetic composition of the present invention is not limited to the one in which only an extract from hanashoubu, iris, oyster butterfly is used as the main agent with regard to prevention and improvement of skin aging.

  Examples of skin cosmetics of the present invention that can be used as constituents of skin cosmetics together with extracts from Japanese iris, iris, and oyster grass include, for example, astringents, bactericides / antibacterial agents, whitening agents, ultraviolet absorbers, and moisturizers. , Cell activators, anti-inflammatory / anti-allergic agents, anti-oxidant / active oxygen scavengers, and the like. The synergistic action between the components used together with the components may lead to better use effects than would normally be expected.

When producing skin cosmetics containing extracts from honeybees, iris, oyster butterflies, the choice of other raw materials is almost unrestricted, such as fats and oils, waxes, hydrocarbons, fatty acids, alcohols Any of general substrates and auxiliaries such as esters, surfactants and fragrances can be used.
The antioxidants, active oxygen scavengers, radical scavengers, and cosmetics containing these described above according to the present invention are preferably applied to humans, but have their respective effects. As long as it is applicable to animals other than humans.

Next, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not limited to each following example at all.
<Production Example 1>
Extraction solvent 1000mL was added to each 100g of chopped roots, stems, and leaves of cherries, extracted at 5 ° C for 48 hours, and filtered to obtain an extract. The residue was further subjected to the same extraction treatment, and the obtained extract and the above extract were combined. The stem / leaf extract was extracted three times with an equal amount of hexane to remove hexane solubles. Each extraction solvent was concentrated under reduced pressure and further dried to obtain an extract. Table 1 shows the extraction rates when water, 50% ethanol (mass ratio of water to ethanol of 1: 1), and ethanol were used as the extraction solvent.

<Production Example 2>
Extraction solution was obtained by adding 1000 mL of extraction solvent to 100 g of each of the roots and stems and leaves of shrimp cut into pieces, and extracting at 5 ° C. for 48 hours, followed by filtration. The residue was further subjected to the same extraction treatment, and the obtained extract and the above extract were combined. The stem / leaf extract was extracted three times with an equal amount of hexane to remove hexane solubles. Each extraction solvent was concentrated under reduced pressure and further dried to obtain an extract. Table 1 shows extraction rates when water, 50% ethanol (mass ratio of water to ethanol of 1: 1), and ethanol were used as the extraction solvent.

<Production Example 3>
To 100 g of oyster grass roots and stems and leaves cut into pieces, 1000 mL of extraction solvent was added, extracted at 5 ° C. for 48 hours, and filtered to obtain an extract. The residue was further subjected to the same extraction treatment, and the obtained extract and the above extract were combined. The stem / leaf extract was extracted three times with an equal amount of hexane to remove hexane solubles. Each extraction solvent was concentrated under reduced pressure and further dried to obtain an extract. Table 1 shows the extraction amount (g) of each extract when water, 50% ethanol (mass ratio of water to ethanol of 1: 1), and ethanol were used as the extraction solvent.

<Test Example 1> Superoxide elimination test (NBT method)
The superoxide scavenging action of the extracts (hereinafter referred to as “samples”) from honey shovel, iris, and oyster butterfly obtained in Production Examples 1, 2, and 3 was tested by the following test method.
3 mM xanthine, 0.05 M sodium carbonate (Na ₂ CO ₃ ) buffer (pH 10.2, 3 mM EDTA, 1.5 mg / mL BSA solution, and 0.75 mM nitroblue tetrazolium (NBT), respectively. Add 0.1 mL of the sample solution to each 0.1 mL test tube, and let stand for 10 minutes at 25 ° C. Next, add 0.1 mL of the xanthine oxidase solution, stir quickly, and let stand at 25 ° C. for 20 minutes. Thereafter, 0.1 mL of 6 mM copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured, which is hereinafter referred to as “absorbance at the time of adding a sample solution and an enzyme solution”.

Further, the same operation and measurement of absorbance were performed without adding the enzyme solution. The absorbance measured at this time is referred to as “absorbance when the sample solution is added and the enzyme solution is not added”.
On the other hand, the same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as “absorbance when no sample solution is added and the enzyme solution is added”.
The same measurement was performed when distilled water was added without adding the enzyme solution and without adding the sample solution. The absorbance measured at this time is referred to as “absorbance when no sample solution is added and no enzyme solution is added”.

The superoxide elimination rate was calculated | required by following Formula using the light absorbency obtained by the said measurement.
Erase rate (%) = (1− (A−B) / (C−D)) × 100
In the above formula, “A” is “absorbance when sample solution is added and enzyme solution is added”, “B” is “absorbance when sample solution is added and enzyme solution is not added”, and “C” is “sample solution is not added and enzyme is added” “Absorptivity when solution is added” and “D” represent “absorbance when sample solution is not added and enzyme solution is not added”.
The above-mentioned erasure rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm: μg / mL) at which the superoxide erasure rate was 50% was determined by interpolation. The results are shown in Table 2.

表２に示す結果から、ハナショウブ、アヤメ、カキツバタの根部、茎・葉部からの抽出物がスーパーオキサイド消去作用を有することが確認された。

From the results shown in Table 2, it was confirmed that extracts from roots, stems, and leaves of Japanese iris, iris, oyster butterfly have superoxide scavenging action.

<Test Example 2> Radical scavenging test for DPPH The following tests were conducted on extracts (hereinafter referred to as “samples”) from the roots, stems, and leaves of Japanese red pepper, iris, oyster butterfly obtained in Production Examples 1, 2, and 3. The radical scavenging action was tested using DPPH, a very stable radical.
3 mL of the sample solution was added to 3 mL of 1.5 × 10 ⁻⁴ M DPPH ethanol solution, and the container was immediately sealed, shaken and allowed to stand for 30 minutes. Thereafter, the absorbance at a wavelength of 526 nm was measured. As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 526 nm was measured. As a blank, after adding 3 mL of the sample solution to ethanol, the absorbance at a wavelength of 526 nm was measured immediately.

From each measured absorbance, the radical scavenging rate (%) was calculated by the following formula.
Erase rate (%) = (1− (B−C) / A) × 100
In the above formula, “A” represents “control absorbance”, “B” represents “absorbance when a sample solution is added”, and “C” represents “blank absorbance”.
The above-mentioned erasure rate was measured by gradually decreasing the sample concentration, and the sample concentration (ppm; μg / mL) at which the DPPH radical erasure rate was 50% was determined by interpolation. The results are shown in Table 3.

表３に示す結果から、ハナショウブ、アヤメ、カキツバタの根部、茎・葉部からの抽出物がラジカル消去作用を有することが確認された。

From the results shown in Table 3, it was confirmed that the extracts from roots, stems and leaves of Japanese iris, iris, oyster butterfly have a radical scavenging action.

<Formulation example 1>
An emulsion having the following composition was produced by a conventional method.
Extract of root of honey shovel 1.0g
Jojoba oil 4.0g
Placenta extract 0.1g
0.1g magnesium ascorbate phosphate
Prune extract 1.0g
1.0 g of Tonin extract
Kyonin extract 1.0g
Taio extract 0.5g
Cousin extract 0.5g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
2.0g glyceryl monostearate
2.5 g of polyoxyethylene cetyl ether (20E.O.)
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.0g
1,3-butylene glycol 3.0 g
Hinokitiol 0.15g
Fragrance 0.15g
Purified water balance (total amount 100 g)
In place of the “Hanashobu root extract” having the above composition, “Hanashobu stalk / leaf extract”, “Iris root extract”, “Iris stalk / leaf extract”, “Oyster butterfly extract”, and “Oyster butterfly extract” The “stem / leaf extract” was prepared in the same manner as the other composition.

<Formulation example 2>
A cream having the following composition was produced by a conventional method.
5.0g ethanol extract of root of honey shovel
Liquid paraffin 5.0g
Salami beeswax 4.0g
Cetanol 3.0g
Squalane 10.0g
Lanolin 2.0g
Arbutin 0.1g
Lucinol 0.1g
Twilight extract 0.1g
Ginkgo biloba extract 0.1g
Conchiolin 0.1g
Oat extract 0.1g
Stearic acid 1.0g
1.5 g of polyoxyethylene sorbitan oleate (20E.O.)
3.0g glyceryl monostearate
1,3-butylene glycol 6.0 g
Methyl paraoxybenzoate 0.05g
Fragrance 0.1g
Purified water balance (total amount 100 g)
Instead of “50% ethanol extract of red potato root” having the above composition, “50% ethanol extract of red shoot stem and leaf”, “50% ethanol extract of iris root”, “50% ethanol extract of iris shoot and leaf” The same composition was used in the same manner as “Oyster beetle root 50% ethanol extract” and “Oyster beetle stem and leaf 50% ethanol extract”.

<Formulation example 3>
A pack having the following composition was produced by a conventional method.
0.5 g of ethanol extract from roots of hanashobu
Polyvinyl alcohol 15.0g
Polyethylene glycol 3.0g
Propylene glycol 7.0g
Ethanol 10.0g
Chamomile extract 0.1g
Ellagic acid 0.1g
Oil-soluble licorice extract 0.1g
Seaweed extract 0.1g
Yeast extract 0.1g
Perilla extract 0.1g
0.1g of bitumen extract
Linden extract 0.1g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water balance (total amount is 100 g)
In place of the “Hanashobu root ethanol extract” having the above composition, “Hanashobu stem / leaf ethanol extract”, “Iris root ethanol extract”, “Iris stem / leaf ethanol extract”, “Oyster root extract ethanol” ”And“ Oyster butterfly stem / leaf ethanol extract ”were prepared in the same manner except that the other compositions were the same.

<Formulation Example 1> formulated with "Hanashobu Root Ethanol Extract", <Formulation Example 1> formulated with "Hanashobu stem / leaf ethanol extract" instead of "Hanashobu Root Ethanol Extract" in <Formulation Example 1>(2)>,<Formulation example 1- (3)> blended with “Iris root extract”, “Formulation 1- (4)>” blended with “Iris stem / leaf ethanol extract”, From <Formulation Example 1- (5)> formulated with "Root ethanol extract", <Formulation Example 1- (6)>, and <Formulation example 1> blended with "Oyster stalk / leaf ethanol extract" For each sample of <Comparative Example 1> excluding the “ethanol extract”, a group of 20 male and female panelists in the 40-60s who have noticeable skin sagging, skin firmness, rough skin, and skin blemishes. , Let it be used for one month in a blind, Changes were evaluated before and after the skin condition by visual observation. As skin symptom indicators, skin sagging, firmness, rough skin, and skin blemishes were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”.
Table 4 shows a summary of the number of panelists obtained for each of the above indices. Table 5 shows the average value ± standard deviation when calculated with 2 points for “improvement”, 1 point for “slightly improved”, and 0 point for “no change”.

表４及び表５に示すように、配合例１〜配合例１−(6)と比較例１との間に有意な差違が認められた。

As shown in Table 4 and Table 5, a significant difference was recognized between Formulation Example 1 to Formulation Example 1- (6) and Comparative Example 1.

  As described above, according to the present embodiment, the production of lipid peroxide is suppressed by inhibiting the generation of active oxygen and in vivo radicals by using the extract from hanashobu, iris, oyster butterfly, and the elasticity of the skin is reduced. We were able to provide a product capable of preventing and treating skin aging, skin inflammation, skin pigmentation, etc.

  Antioxidants, active oxygen scavengers and radical scavengers of the present invention prevent skin aging, skin inflammation and skin pigmentation through prevention of oxidation of biological components by active oxygen scavenging action and in vivo radical scavenging action And / or useful to improve.

Claims (5)

It contains an extract of one or more plants selected from Iris ensata var. Ensata, Iris sanguinea Hornem. And Iris laevigata Fisch. As an active ingredient. Antioxidants. The antioxidant according to claim 1, wherein the extract has an active oxygen scavenging action and / or a radical scavenging action. A radical scavenger comprising the antioxidant according to claim 2 as an active ingredient. An active oxygen scavenger comprising the antioxidant according to claim 2 as an active ingredient. A cosmetic comprising the antioxidant according to any one of claims 1 to 4.