JP2019513387A5 - - Google Patents
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- Publication number
- JP2019513387A5 JP2019513387A5 JP2018553370A JP2018553370A JP2019513387A5 JP 2019513387 A5 JP2019513387 A5 JP 2019513387A5 JP 2018553370 A JP2018553370 A JP 2018553370A JP 2018553370 A JP2018553370 A JP 2018553370A JP 2019513387 A5 JP2019513387 A5 JP 2019513387A5
- Authority
- JP
- Japan
- Prior art keywords
- nanopore
- fluorescent
- polymer
- optical
- labeled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims description 33
- 239000002773 nucleotide Substances 0.000 claims description 26
- 125000003729 nucleotide group Chemical group 0.000 claims description 26
- 230000003287 optical effect Effects 0.000 claims description 24
- 102000040430 polynucleotide Human genes 0.000 claims description 21
- 108091033319 polynucleotide Proteins 0.000 claims description 21
- 239000002157 polynucleotide Substances 0.000 claims description 21
- 239000007850 fluorescent dye Substances 0.000 claims description 20
- 239000000178 monomer Substances 0.000 claims description 16
- 229920000642 polymer Polymers 0.000 claims description 12
- 230000005284 excitation Effects 0.000 claims description 10
- 238000010791 quenching Methods 0.000 claims description 7
- 230000000171 quenching effect Effects 0.000 claims description 7
- 239000002777 nucleoside Substances 0.000 claims description 6
- -1 nucleoside triphosphates Chemical class 0.000 claims description 6
- 235000011178 triphosphate Nutrition 0.000 claims description 6
- 239000001226 triphosphate Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 2
- 108020004707 nucleic acids Proteins 0.000 claims description 2
- 150000007523 nucleic acids Chemical class 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 230000003993 interaction Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662322343P | 2016-04-14 | 2016-04-14 | |
| US62/322,343 | 2016-04-14 | ||
| PCT/US2017/024314 WO2017180319A1 (en) | 2016-04-14 | 2017-03-27 | Mixed optical signals in polymer analysis with nanopores |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019513387A JP2019513387A (ja) | 2019-05-30 |
| JP2019513387A5 true JP2019513387A5 (enExample) | 2020-05-07 |
Family
ID=60042715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018553370A Pending JP2019513387A (ja) | 2016-04-14 | 2017-03-27 | ナノポアを用いるポリマー分析における混合された光学的信号 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US20190078158A1 (enExample) |
| EP (1) | EP3443122B1 (enExample) |
| JP (1) | JP2019513387A (enExample) |
| CN (1) | CN109415765A (enExample) |
| WO (1) | WO2017180319A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109804087A (zh) * | 2016-08-19 | 2019-05-24 | 昆塔波尔公司 | 使用猝灭剂的基于光学的纳米孔测序 |
| GB201902630D0 (en) * | 2019-02-27 | 2019-04-10 | Imperial Innovations Ltd | Analyte detection method |
| US20230193376A1 (en) | 2020-04-24 | 2023-06-22 | Quantapore, Inc. | Fluorescent polynucleotide sequencing methods and compositions |
| WO2023029044A1 (zh) * | 2021-09-06 | 2023-03-09 | 百图生科(北京)智能技术有限公司 | 用于单细胞测序的方法、装置、设备、介质和程序产品 |
| GB202207267D0 (en) * | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7575865B2 (en) * | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| WO2011040996A1 (en) * | 2009-09-30 | 2011-04-07 | Quantapore, Inc. | Ultrafast sequencing of biological polymers using a labeled nanopore |
| WO2012121756A1 (en) * | 2011-03-04 | 2012-09-13 | Quantapore, Inc. | Apparatus and methods for performing optical nanopore detection or sequencing |
| US20130040827A1 (en) * | 2011-08-14 | 2013-02-14 | Stephen C. Macevicz | Method and compositions for detecting and sequencing nucleic acids |
| WO2014066905A2 (en) * | 2012-10-28 | 2014-05-01 | Quantapore, Inc. | Reducing background fluorescence in mems materials by low energy ion beam treatment |
| US9862997B2 (en) * | 2013-05-24 | 2018-01-09 | Quantapore, Inc. | Nanopore-based nucleic acid analysis with mixed FRET detection |
| EP3204519B1 (en) * | 2014-10-10 | 2020-04-29 | Quantapore Inc. | Nanopore-based polymer analysis with mutually-quenching fluorescent labels |
| US20170132361A1 (en) * | 2015-10-28 | 2017-05-11 | Pacific Biosciences Of California, Inc. | Sequence assembly method |
| CN108463560B (zh) * | 2016-01-15 | 2022-09-20 | 昆塔波尔公司 | 具有降低的背景的基于光学的纳米孔分析 |
| JP2019528776A (ja) * | 2016-10-10 | 2019-10-17 | クアンタポール， インコーポレイテッド | 光学に基づくナノポア配列決定における蛍光標識組成物の制御 |
-
2017
- 2017-03-27 CN CN201780023542.2A patent/CN109415765A/zh active Pending
- 2017-03-27 WO PCT/US2017/024314 patent/WO2017180319A1/en not_active Ceased
- 2017-03-27 US US16/085,974 patent/US20190078158A1/en not_active Abandoned
- 2017-03-27 EP EP17782828.2A patent/EP3443122B1/en active Active
- 2017-03-27 JP JP2018553370A patent/JP2019513387A/ja active Pending
-
2021
- 2021-09-08 US US17/447,143 patent/US20220170088A1/en not_active Abandoned
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