JP2019511208A - ウイルスを作製する方法 - Google Patents
ウイルスを作製する方法 Download PDFInfo
- Publication number
- JP2019511208A JP2019511208A JP2018541216A JP2018541216A JP2019511208A JP 2019511208 A JP2019511208 A JP 2019511208A JP 2018541216 A JP2018541216 A JP 2018541216A JP 2018541216 A JP2018541216 A JP 2018541216A JP 2019511208 A JP2019511208 A JP 2019511208A
- Authority
- JP
- Japan
- Prior art keywords
- virus
- cell culture
- japanese eel
- genus
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 229960005486 vaccine Drugs 0.000 claims abstract description 13
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- 241000252084 Anguilla Species 0.000 claims description 45
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- 229930182566 Gentamicin Natural products 0.000 description 5
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- 229930182816 L-glutamine Natural products 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
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- 229940030156 cell vaccine Drugs 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 208000009663 Acute Necrotizing Pancreatitis Diseases 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
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- 239000000725 suspension Substances 0.000 description 2
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- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 1
- 241000252073 Anguilliformes Species 0.000 description 1
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- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 241000721654 Lepomis macrochirus Species 0.000 description 1
- 102100038750 Myc-associated zinc finger protein Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 241000524174 Nova virus Species 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 241000709664 Picornaviridae Species 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
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- 230000002068 genetic effect Effects 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229940031551 inactivated vaccine Drugs 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000001320 lysogenic effect Effects 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
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- 238000012360 testing method Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000007502 viral entry Effects 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/10011—Birnaviridae
- C12N2720/10061—Methods of inactivation or attenuation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/30011—Nodaviridae
- C12N2770/30034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/30011—Nodaviridae
- C12N2770/30051—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2796/00—Viruses not covered by groups C12N2710/00 - C12N2795/00
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1601861.6A GB201601861D0 (en) | 2016-02-02 | 2016-02-02 | Methods of producing viruses |
| GB1601861.6 | 2016-02-02 | ||
| GB1618549.8 | 2016-11-03 | ||
| GB201618549 | 2016-11-03 | ||
| PCT/GB2017/050259 WO2017134441A1 (en) | 2016-02-02 | 2017-02-02 | Methods of producing viruses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019511208A true JP2019511208A (ja) | 2019-04-25 |
| JP2019511208A5 JP2019511208A5 (enExample) | 2020-03-12 |
Family
ID=58018136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018541216A Abandoned JP2019511208A (ja) | 2016-02-02 | 2017-02-02 | ウイルスを作製する方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10450544B2 (enExample) |
| EP (1) | EP3411064B1 (enExample) |
| JP (1) | JP2019511208A (enExample) |
| CN (1) | CN109414494A (enExample) |
| AU (1) | AU2017215287A1 (enExample) |
| BR (1) | BR112018015527A2 (enExample) |
| CA (1) | CA3011920A1 (enExample) |
| CL (1) | CL2018002042A1 (enExample) |
| ES (1) | ES2795040T3 (enExample) |
| SG (1) | SG11201806182RA (enExample) |
| WO (1) | WO2017134441A1 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111118171A (zh) * | 2019-12-31 | 2020-05-08 | 广东省测试分析研究所（中国广州分析测试中心） | 一种鉴别鱼翅种的dna条形码引物对、试剂盒及方法 |
| CN113122510A (zh) * | 2021-04-12 | 2021-07-16 | 中国水产科学研究院黑龙江水产研究所 | 一种传染性造血器官坏死病疫苗及其病毒在胖头鱥肌肉细胞上扩增的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5780448A (en) * | 1995-11-07 | 1998-07-14 | Ottawa Civic Hospital Loeb Research | DNA-based vaccination of fish |
| TWI220904B (en) * | 1998-12-03 | 2004-09-11 | Nat Science Council | An immortal cell line derived from grouper Epinephelus coioides and its applications therein |
| TWI347364B (en) * | 2003-02-19 | 2011-08-21 | Novartis Ag | Inactivated nodavirus vaccine |
| NO332608B1 (no) | 2011-03-16 | 2012-11-19 | Norwegian School Of Veterinary Science | Levende avirulent IPNV og vaksine for profylakse eller behandling av IPN-sykdom hos fisk |
| CN104531608A (zh) * | 2015-01-12 | 2015-04-22 | 福建省农业科学院生物技术研究所 | 一种欧洲鳗鲡肝脏细胞系及其构建方法和应用 |
| CN105192298A (zh) * | 2015-11-11 | 2015-12-30 | 浙江锦天生物科技有限公司 | 一种日本鳗鲡用复合微生态饲料添加剂 |
-
2017
- 2017-02-02 ES ES17704811T patent/ES2795040T3/es active Active
- 2017-02-02 CA CA3011920A patent/CA3011920A1/en not_active Abandoned
- 2017-02-02 AU AU2017215287A patent/AU2017215287A1/en not_active Abandoned
- 2017-02-02 WO PCT/GB2017/050259 patent/WO2017134441A1/en not_active Ceased
- 2017-02-02 EP EP17704811.3A patent/EP3411064B1/en active Active
- 2017-02-02 JP JP2018541216A patent/JP2019511208A/ja not_active Abandoned
- 2017-02-02 BR BR112018015527A patent/BR112018015527A2/pt not_active IP Right Cessation
- 2017-02-02 CN CN201780009494.1A patent/CN109414494A/zh active Pending
- 2017-02-02 US US16/074,501 patent/US10450544B2/en not_active Expired - Fee Related
- 2017-02-02 SG SG11201806182RA patent/SG11201806182RA/en unknown
-
2018
- 2018-07-30 CL CL2018002042A patent/CL2018002042A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017134441A1 (en) | 2017-08-10 |
| BR112018015527A2 (pt) | 2018-12-26 |
| US10450544B2 (en) | 2019-10-22 |
| CA3011920A1 (en) | 2017-08-10 |
| EP3411064A1 (en) | 2018-12-12 |
| CN109414494A (zh) | 2019-03-01 |
| CL2018002042A1 (es) | 2019-03-22 |
| ES2795040T3 (es) | 2020-11-20 |
| SG11201806182RA (en) | 2018-08-30 |
| US20190040354A1 (en) | 2019-02-07 |
| AU2017215287A1 (en) | 2018-08-16 |
| EP3411064B1 (en) | 2020-02-26 |
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