JP2019510494A5 - - Google Patents
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- Publication number
- JP2019510494A5 JP2019510494A5 JP2018549769A JP2018549769A JP2019510494A5 JP 2019510494 A5 JP2019510494 A5 JP 2019510494A5 JP 2018549769 A JP2018549769 A JP 2018549769A JP 2018549769 A JP2018549769 A JP 2018549769A JP 2019510494 A5 JP2019510494 A5 JP 2019510494A5
- Authority
- JP
- Japan
- Prior art keywords
- fluorescent
- protein
- seq
- amino acid
- acid sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003550 marker Substances 0.000 claims description 52
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 37
- 108020001507 fusion proteins Proteins 0.000 claims description 22
- 102000037865 fusion proteins Human genes 0.000 claims description 22
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 108090000623 proteins and genes Proteins 0.000 claims description 21
- 102000029749 Microtubule Human genes 0.000 claims description 20
- 108091022875 Microtubule Proteins 0.000 claims description 20
- 210000004688 microtubule Anatomy 0.000 claims description 20
- 108091006047 fluorescent proteins Proteins 0.000 claims description 16
- 102000034287 fluorescent proteins Human genes 0.000 claims description 16
- 239000000758 substrate Substances 0.000 claims description 15
- 239000007850 fluorescent dye Substances 0.000 claims description 14
- 238000000799 fluorescence microscopy Methods 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 102000004243 Tubulin Human genes 0.000 claims description 12
- 108090000704 Tubulin Proteins 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 9
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims description 8
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims description 8
- 101710172711 Structural protein Proteins 0.000 claims description 8
- 239000005090 green fluorescent protein Substances 0.000 claims description 8
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
- 102000035181 adaptor proteins Human genes 0.000 claims description 4
- 108091005764 adaptor proteins Proteins 0.000 claims description 4
- 208000036815 beta tubulin Diseases 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 4
- 102000040430 polynucleotide Human genes 0.000 claims description 4
- 108091033319 polynucleotide Proteins 0.000 claims description 4
- 239000002157 polynucleotide Substances 0.000 claims description 4
- 210000000979 axoneme Anatomy 0.000 claims description 3
- 210000003495 flagella Anatomy 0.000 claims description 3
- 239000000833 heterodimer Substances 0.000 claims description 2
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
- 210000003050 axon Anatomy 0.000 claims 1
- 238000000386 microscopy Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662312772P | 2016-03-24 | 2016-03-24 | |
| US62/312,772 | 2016-03-24 | ||
| PCT/US2017/024051 WO2017165788A1 (en) | 2016-03-24 | 2017-03-24 | Quantitative flagellar fluorescent markers and standards |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019510494A JP2019510494A (ja) | 2019-04-18 |
| JP2019510494A5 true JP2019510494A5 (https=) | 2020-04-30 |
Family
ID=59899747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018549769A Pending JP2019510494A (ja) | 2016-03-24 | 2017-03-24 | 定量的鞭毛蛍光マーカーおよび標準物質 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20190011368A1 (https=) |
| EP (1) | EP3433263A4 (https=) |
| JP (1) | JP2019510494A (https=) |
| CN (1) | CN109071609A (https=) |
| AU (1) | AU2017237163B2 (https=) |
| CA (1) | CA3018572A1 (https=) |
| WO (1) | WO2017165788A1 (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20230340036A1 (en) * | 2020-02-03 | 2023-10-26 | The Regents Of The University Of California | Heterologous proteins with axonemal proteins |
| CN115991757B (zh) * | 2022-07-15 | 2023-08-11 | 南宁师范大学 | 信号增强型检测汞离子的荧光蛋白探针及其制备与应用 |
| CN116309723A (zh) * | 2023-03-20 | 2023-06-23 | 中国科学院深圳先进技术研究院 | 一种基于细菌鞭毛染色图像的轨迹确定方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US5264618A (en) | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
| WO1991017424A1 (en) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
| US5948677A (en) * | 1996-12-09 | 1999-09-07 | Jarvik; Jonathan W. | Reading frame independent epitope tagging |
| EP1009853B1 (en) * | 1997-09-04 | 2006-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Assays for detecting modulators of cytoskeletal function |
| US7396664B2 (en) | 1999-06-08 | 2008-07-08 | Regeneron Pharmaceuticals, Inc. | VEGF-binding fusion proteins and nucleic acids encoding the same |
| JP2005537784A (ja) * | 2002-04-23 | 2005-12-15 | ザ スクリップス リサーチ インスティテュート | 葉緑体におけるポリペプチドの発現、ならびにそのポリペプチドを発現するための組成物および方法 |
| US20040180378A1 (en) * | 2002-07-19 | 2004-09-16 | Eileen Tozer | Fluorescent proteins, nucleic acids encoding them and methods for making and using them |
| EP1997885A1 (en) * | 2007-05-29 | 2008-12-03 | AXXAM S.p.A. | Fluorescent proteins and methods of use thereof |
| GB201718294D0 (en) * | 2017-11-03 | 2017-12-20 | Nextera As | Vector construct |
-
2017
- 2017-03-24 WO PCT/US2017/024051 patent/WO2017165788A1/en not_active Ceased
- 2017-03-24 CN CN201780025441.9A patent/CN109071609A/zh active Pending
- 2017-03-24 AU AU2017237163A patent/AU2017237163B2/en not_active Ceased
- 2017-03-24 CA CA3018572A patent/CA3018572A1/en not_active Abandoned
- 2017-03-24 EP EP17771246.0A patent/EP3433263A4/en not_active Withdrawn
- 2017-03-24 JP JP2018549769A patent/JP2019510494A/ja active Pending
-
2018
- 2018-09-24 US US16/139,508 patent/US20190011368A1/en not_active Abandoned
-
2021
- 2021-01-05 US US17/142,090 patent/US20210148826A1/en not_active Abandoned
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