JP2019500910A5 - - Google Patents
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- JP2019500910A5 JP2019500910A5 JP2018555445A JP2018555445A JP2019500910A5 JP 2019500910 A5 JP2019500910 A5 JP 2019500910A5 JP 2018555445 A JP2018555445 A JP 2018555445A JP 2018555445 A JP2018555445 A JP 2018555445A JP 2019500910 A5 JP2019500910 A5 JP 2019500910A5
- Authority
- JP
- Japan
- Prior art keywords
- ebna
- reprogramming
- vector
- cell population
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000008672 reprogramming Effects 0.000 claims 57
- 239000013598 vector Substances 0.000 claims 45
- 210000004027 cell Anatomy 0.000 claims 43
- 108010031111 EBV-encoded nuclear antigen 1 Proteins 0.000 claims 34
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 25
- 108090000623 proteins and genes Proteins 0.000 claims 19
- 238000012258 culturing Methods 0.000 claims 16
- 238000000034 method Methods 0.000 claims 12
- 102000040945 Transcription factor Human genes 0.000 claims 11
- 108091023040 Transcription factor Proteins 0.000 claims 11
- 230000010076 replication Effects 0.000 claims 8
- 210000001082 somatic cell Anatomy 0.000 claims 7
- 210000001671 embryonic stem cell Anatomy 0.000 claims 6
- 238000004519 manufacturing process Methods 0.000 claims 6
- 101100257359 Caenorhabditis elegans sox-2 gene Proteins 0.000 claims 5
- 102000000311 Cytosine Deaminase Human genes 0.000 claims 5
- 108010080611 Cytosine Deaminase Proteins 0.000 claims 5
- 101100257363 Mus musculus Sox2 gene Proteins 0.000 claims 5
- 239000004098 Tetracycline Substances 0.000 claims 5
- 102000006601 Thymidine Kinase Human genes 0.000 claims 5
- 108020004440 Thymidine kinase Proteins 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 5
- 108091033319 polynucleotide Proteins 0.000 claims 5
- 239000002157 polynucleotide Substances 0.000 claims 5
- 229960002180 tetracycline Drugs 0.000 claims 5
- 229930101283 tetracycline Natural products 0.000 claims 5
- 235000019364 tetracycline Nutrition 0.000 claims 5
- 150000003522 tetracyclines Chemical class 0.000 claims 5
- 108020005091 Replication Origin Proteins 0.000 claims 4
- 230000001276 controlling effect Effects 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 101100239628 Danio rerio myca gene Proteins 0.000 claims 3
- 101001139134 Homo sapiens Krueppel-like factor 4 Proteins 0.000 claims 3
- 102100020677 Krueppel-like factor 4 Human genes 0.000 claims 3
- 101150111214 lin-28 gene Proteins 0.000 claims 3
- 230000001105 regulatory effect Effects 0.000 claims 3
- 238000012216 screening Methods 0.000 claims 3
- -1 Nanog Proteins 0.000 claims 2
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 claims 2
- 241000700605 Viruses Species 0.000 claims 2
- 230000004913 activation Effects 0.000 claims 2
- 230000032823 cell division Effects 0.000 claims 2
- 238000011529 RT qPCR Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 210000002950 fibroblast Anatomy 0.000 claims 1
- 230000002496 gastric effect Effects 0.000 claims 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims 1
- 210000003494 hepatocyte Anatomy 0.000 claims 1
- 210000002510 keratinocyte Anatomy 0.000 claims 1
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662277784P | 2016-01-12 | 2016-01-12 | |
| US62/277,784 | 2016-01-12 | ||
| PCT/US2017/013229 WO2017123789A1 (en) | 2016-01-12 | 2017-01-12 | Methods and vectors to produce vector free induced pluripotent stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2019500910A JP2019500910A (ja) | 2019-01-17 |
| JP2019500910A5 true JP2019500910A5 (https=) | 2020-02-20 |
Family
ID=59311564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018555445A Pending JP2019500910A (ja) | 2016-01-12 | 2017-01-12 | ベクターを含まない人工多能性幹細胞を作製するための方法およびベクター |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170226483A1 (https=) |
| EP (1) | EP3402496A4 (https=) |
| JP (1) | JP2019500910A (https=) |
| KR (1) | KR20180105670A (https=) |
| CN (1) | CN108778299A (https=) |
| CA (1) | CA3010764A1 (https=) |
| IL (1) | IL260452A (https=) |
| WO (1) | WO2017123789A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017123789A1 (en) * | 2016-01-12 | 2017-07-20 | Lonza Walkersville, Inc. | Methods and vectors to produce vector free induced pluripotent stem cells |
| JP7554670B2 (ja) * | 2017-10-11 | 2024-09-20 | フェイト セラピューティクス，インコーポレイテッド | 一時的かつ一過性プラスミドベクター発現システムを用いる細胞のリプログラミング |
| CN108373998B (zh) * | 2018-02-10 | 2019-08-27 | 安徽中盛溯源生物科技有限公司 | 一种通过蝾螈Oct4将人类血液细胞重编程为iPSC的方法 |
| GB201805683D0 (en) * | 2018-04-05 | 2018-05-23 | Touchlight Ip Ltd | Reprogramming vectors |
| US12385047B2 (en) | 2018-06-18 | 2025-08-12 | Pearl Kogyo Co., Ltd. | Transformed cell production method |
| CN109679994B (zh) * | 2018-12-13 | 2021-02-02 | 湖北汇智铭传生物科技股份有限公司 | 通过四环素诱导表达外源基因的游离载体及其构建方法 |
| AU2021353586A1 (en) * | 2020-10-02 | 2023-05-11 | Fate Therapeutics, Inc. | Improved reprogramming, maintenance and preservation for induced pluripotent stem cells |
| WO2023064572A2 (en) * | 2021-10-15 | 2023-04-20 | Agex Therapeutics, Inc. | Methods for the temporal regulation of reprogramming factors in mammalian cells |
| CN114277190B (zh) * | 2021-12-31 | 2024-07-23 | 安徽中盛溯源生物科技有限公司 | 一种hiPSC中外源基因残留检测用特异性DNA片段、引物、试剂盒和检测方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2008295441A1 (en) * | 2007-09-04 | 2009-03-12 | Queensland University Of Technology | A feeder cell-free culture medium and system |
| EP3279314A1 (en) * | 2008-06-04 | 2018-02-07 | Cellular Dynamics International, Inc. | Methods for the production of ips cells using non-viral approach |
| JP2012518409A (ja) * | 2009-02-20 | 2012-08-16 | ベントリア・バイオサイエンス | タンパク質の組み合わせを含有する細胞培養培地 |
| WO2010141801A2 (en) * | 2009-06-05 | 2010-12-09 | Cellular Dynamics International, Inc. | Reprogramming t cells and hematophietic cells |
| WO2011056971A2 (en) * | 2009-11-04 | 2011-05-12 | Cellular Dynamics International, Inc. | Episomal reprogramming with chemicals |
| EP2582793B1 (en) * | 2010-06-15 | 2017-09-06 | Cellular Dynamics International, Inc. | A compendium of ready-built stem cell models for interrogation of biological response |
| CA2806858C (en) * | 2010-08-04 | 2021-06-15 | Cellular Dynamics International, Inc. | Reprogramming immortalized b cells |
| JP2014520551A (ja) * | 2011-07-11 | 2014-08-25 | セルラー ダイナミクス インターナショナル， インコーポレイテッド | 細胞のリプログラミング方法およびゲノムの改変方法 |
| WO2013177228A1 (en) * | 2012-05-22 | 2013-11-28 | Loma Linda University | Generation of integration/transgene-free stem cells |
| AU2013264708B2 (en) * | 2012-05-23 | 2019-03-07 | Kyoto University | Highly efficient method for establishing artificial pluripotent stem cell |
| US10738323B2 (en) * | 2013-07-12 | 2020-08-11 | Cedars-Sinai Medical Center | Generation of induced pluripotent stem cells from normal human mammary epithelial cells |
| CA2941004A1 (en) * | 2014-03-04 | 2015-09-11 | Peter Flynn | Improved reprogramming methods and cell culture platforms |
| US20160362705A1 (en) * | 2015-06-12 | 2016-12-15 | Lonza Walkersville, Inc. | Methods for Nuclear Reprogramming Using Synthetic Transcription Factors |
| AU2016318774B2 (en) * | 2015-09-08 | 2022-08-18 | FUJIFILM Cellular Dynamics, Inc. | MACS-based purification of stem cell-derived retinal pigment epithelium |
| US10947502B2 (en) * | 2015-10-20 | 2021-03-16 | FUJIFILM Cellular Dynamics, Inc. | Methods for directed differentiation of pluripotent stem cells to immune cells |
| WO2017123789A1 (en) * | 2016-01-12 | 2017-07-20 | Lonza Walkersville, Inc. | Methods and vectors to produce vector free induced pluripotent stem cells |
| JP7554670B2 (ja) * | 2017-10-11 | 2024-09-20 | フェイト セラピューティクス，インコーポレイテッド | 一時的かつ一過性プラスミドベクター発現システムを用いる細胞のリプログラミング |
| CN108410823B (zh) * | 2018-03-26 | 2019-11-01 | 安徽中盛溯源生物科技有限公司 | 一种微环游离型载体高效重编程血液细胞生成iPSC的方法 |
-
2017
- 2017-01-12 WO PCT/US2017/013229 patent/WO2017123789A1/en not_active Ceased
- 2017-01-12 CN CN201780016762.2A patent/CN108778299A/zh active Pending
- 2017-01-12 CA CA3010764A patent/CA3010764A1/en not_active Abandoned
- 2017-01-12 EP EP17738957.4A patent/EP3402496A4/en not_active Withdrawn
- 2017-01-12 JP JP2018555445A patent/JP2019500910A/ja active Pending
- 2017-01-12 US US15/404,960 patent/US20170226483A1/en not_active Abandoned
- 2017-01-12 KR KR1020187023128A patent/KR20180105670A/ko not_active Withdrawn
-
2018
- 2018-07-06 IL IL260452A patent/IL260452A/en unknown
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