JP2019180345A - Vitamin A metabolism promoter - Google Patents

Abstract

To provide a vitamin A metabolism promoter that improves vitamin A metabolism in liver and can also improve dyslipidemia.SOLUTION: The present invention provides a vitamin A metabolism promoter that contains pregelatinized brown rice as an active ingredient.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a vitamin A metabolism promoter that enhances the metabolism of vitamin A in the liver.

  Obesity due to westernization and lack of exercise not only causes dyslipidemia, hypertension, and type 2 diabetes, but obesity affects non-alcoholic fatty liver (NAFLD) and affects 10-30% of Japanese people. It has been estimated that it is transformed into liver cancer via non-alcoholic steatohepatitis (NASH). Lifestyle-related diseases are a general term for diseases in which lifestyle-related diseases are thought to be deeply involved in the cause of onset, such as diabetes, dyslipidemia, hypertension, and hyperuricemia. Since it is said that the risk of three major causes of death, cancer, cerebrovascular disease and heart disease, is urgently required.

  In particular, liver dysfunction is a factor that causes damage to various functions such as metabolic function, detoxification, energy storage, bile production, etc., and so far has been studied from various aspects and proposed solutions. ing.

  For example, Japanese Patent Application Laid-Open No. 2009-221193 provides a new use of a rice-derived peptide by a method comprising a step of treating sake lees with cellulase and β-amylase and then treating with protease N Amano G and Sumiteam AP. A liver dysfunction inhibitor comprising a peptide mixture obtained by a method comprising treating a peptide mixture or sake lees obtained with cellulase and β-amylase and then treating with samoylase is disclosed (Patent Document 1).

JP 2009-221193 A

  It is known that abnormal lipid metabolism is caused by vitamin A deficiency. Therefore, if vitamin A metabolism in the liver can be improved, various diseases caused by abnormal vitamin A metabolism are expected to be improved.

  Accordingly, an object of the present invention is to provide a vitamin A metabolism promoter that improves vitamin A metabolism in the liver.

  As a result of intensive studies to solve the above problems, the present inventors have found that ingestion of pregelatinized brown rice increases vitamin A metabolism and improves lipid metabolism abnormalities, and has completed the present invention. It was.

  The present invention has been made based on such findings, and provides a vitamin A metabolism promoter characterized by containing pregelatinized brown rice as an active ingredient.

  According to the present invention, since vitamin A metabolism in the liver is increased by ingesting a vitamin A metabolism promoter, various diseases (visual effects, dermatitis, poor growth, lipids) caused by a decrease in vitamin A metabolism are caused. It can be applied to prevention, improvement, treatment, etc. of metabolic abnormalities).

It is a figure which shows the result of having measured the liver alpha SMA gene expression level in the rat which ingested pregelatinized brown rice. It is a figure which shows the result of having measured the liver retinyl palmitate density | concentration (a) and the LRAT gene expression level (b) in the rat which ingested pregelatinized brown rice. It is a figure which shows the result of having measured the Raldh1 gene expression level (a) and RAR (beta) gene expression level (b) in the rat which ingested pregelatinized brown rice. It is a figure for demonstrating the CPT1 and MTP effect with respect to (beta) oxidation and steatosis.

  Hereinafter, embodiments according to the present invention will be described in detail. In the present embodiment, “brown rice” is rice in a state in which the rice husk covering the outside is removed, and is made of rice bran (fruit skin, seed coat, glue layer), endosperm (white rice), and germ. . The varieties and types of rice are not particularly limited, and glutinous rice, glutinous rice, germinated brown rice, germinated rice, split rice, black rice, red rice, and blended rice obtained by blending two or more of these can be used.

  In this embodiment, the brown rice used is pregelatinized brown rice. There are no particular limitations on the method of pregelatinization, and those used in ordinary brown rice cooking can be used, and examples include direct fire heating and steam heating. Heat processing conditions should just be the conditions which make brown rice into a rice cooking state (alpha-ized state) uniformly, and are 10 to 60 minutes (preferably 20 to 50 minutes) at 90-135 degreeC (preferably 98-125 degreeC). Although preferred, this range is not limited.

  In the present embodiment, the brown rice may be the same as maintaining the shape of the brown rice, but may be a pulverized product such as a coarsely pulverized or finely pulverized brown rice, a mixture of brown rice and pulverized material, and a mixture of various pulverized products. But you can.

  The vitamin A metabolism promoter according to the present embodiment can be formulated using a known formulation method, particularly a formulation technique suitable for oral intake. Examples of the preparation include tablet-coated tablets, capsules, granules, fine granules, powders, emulsions, syrups and the like.

  The vitamin A metabolism promoter of this embodiment can be blended with various food ingredients or additives as long as the object of the present invention is not impaired. Preferred food ingredients or additives include, for example, beta-carotene, which is a precursor of vitamin A, oil that promotes absorption of vitamin A, white rice (because white rice has some effects and is easier to eat than brown rice alone) Etc.

1. Materials and methods
6 weeks old male Zucker fatty rats, wild type male Zucker Lean rats were pre-bred for 4 days with AIN93G, then divided into 4 experimental groups: Lean group, control group, WR (white rice), BR (brown rice), They were raised for 10 weeks (Table 1). Feed composition is Lean group, control group is AIN93G, WR or BR is AIN93G as basic composition. % By weight) was adjusted. Powders obtained by using alpha food products supplied from Alpha Foods Co., Ltd. and pulverizing them to a particle size of 150 μm or less (100 mesh pass) using a jet crusher (Seishin Industry Co., Ltd.). Was used for feed. Zucker fatty rat is an obese model animal that develops NAFLD, and AIN-93G is a standard refinement for nutritional research for mice and rats published in 1993 by the American Institute of Nutrition. Feed composition.

  Retinyl palmitate of vitamin A metabolite was measured by HPLC (manufactured by Shimadzu Corporation, type CTO-10ASVP). For HPLC conditions, column: Wakosil II 5C18AR; 4.6 mm x 250 mm, Mightysil RP-18GP II 150-4.6, flow rate 0.8 ml / min, solvent ethanol: water = 95: 5, detection is fluorescence excitation wavelength (325 nm) absorption Performed at a wavelength (470 nm). Liver αSMA, LRAT, vitamin A metabolizing enzyme Raldh1, retinoic acid receptor β (RARβ), β-oxidation-related factor nuclear receptor PPARα, mitochondrial β-oxidation rate-limiting enzyme CPT1a and insulin response factor The gene expression level of MTP was analyzed by real-time PCR (Applied Biotechnology, format StepOne).

2. Results FIG. 1 is a diagram showing the results of measuring the expression level of liver αSMA gene in rats fed with pregelatinized brown rice. BR liver αSMA gene expression was significantly reduced compared to the control group (p <0.05).

  FIG. 2 is a graph showing the results of measurement of liver retinyl palmitate concentration (a) and LRAT (b) in rats fed pregelatinized brown rice. FIG. 3 shows Raldh1 gene expression level (a) and RARβ gene. It is a figure which shows the result of having measured the expression level (b).

  As shown in FIGS. 2 and 3, liver retinyl palmitate concentration, Raldh1, and RARβ gene expression were significantly increased compared to the control group (p <0.05). Moreover, it turned out that the metabolism of vitamin A is accelerated | stimulated by the intake of pregelatinized brown rice from the result of FIGS.

  FIG. 4 is a diagram for explaining the effects of CPT1 and MTP on β-oxidation and steatosis. There is a report that an agonist of RARβ2, which is a receptor for retinoic acid, which is a vitamin A active body, activates PPARα and activates fatty acid β oxidation to reduce NAFLD. The RARβ gene expression level correlated with the amount of retinoic acid is significantly increased by brown rice intake compared to the control group (FIG. 2b). In other words, pre-gelatinized brown rice intake increases RARβ, whose expression is controlled by vitamin A metabolism, especially retinoic acid level, and increases the expression levels of β-oxidation-related genes such as PPARα and CPT1a, and is involved in fatty liver improvement The possibility was suggested (Fig. 4a, b). In addition, brown rice intake increases MTP gene expression and promotes the release of neutral fat (TG) into the blood as very low density lipoprotein (VLDL), which is thought to improve NAFLD ( FIG. 4c).

  From the above results, it was found that a vitamin A metabolism improving agent containing pregelatinized brown rice as an active ingredient can improve vitamin A storage in the liver and thereby improve liver lipid metabolism accompanying obesity.

Claims (3)

  A vitamin A metabolism promoter characterized by containing pregelatinized brown rice as an active ingredient.   The vitamin A metabolism promoter according to claim 1, wherein the brown rice is a pulverized pulverized product.   Use of pregelatinized brown rice in which pregelatinized brown rice is used as a vitamin A metabolism promoter.