JP2019137655A - Skin external preparation - Google Patents

Abstract

To provide a novel active ingredient that can be blended in a skin external preparation and is useful for improving skin aging and rough skin, and preventing and improving skin blotches, freckles and chloasma.SOLUTION: The present invention provides a skin external preparation containing yeast (Saccharomyces mikatae) extract, or a concentrate or dry powder thereof as an active ingredient, and a skin external preparation for whitening containing yeast (Saccharomyces mikatae) extract, or a concentrate or dry powder thereof as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a yeast or processed product thereof that can be blended in an external preparation for skin (including scalp).

  Conventionally, ingredients derived from natural products have been researched and developed as active ingredients to be blended with external preparations for skin. However, when these components derived from natural products are used as an active ingredient of an external preparation for skin, there are problems in terms of effectiveness and stability.

  As a result of intensive studies to solve the above problems, the present inventors have newly found that Saccharomyces mikatae, a yeast of the genus Saccharomyces, has an excellent whitening effect.

Conventionally, as a yeast of the genus Saccharomyces, Saccharomyces mikatae is known as a component of an oral composition from, for example, Patent Document 1. However, it has not been known to use this yeast as an active ingredient of an external preparation for skin.
JP2009-298702

The present invention is an external preparation for skin containing as an active ingredient a yeast (Saccharomyces mikatae) extract, or a concentrate or dry powder thereof.
The present invention also provides a skin whitening external preparation containing, as an active ingredient, a yeast (Saccharomyces mikatae) extract, a concentrate thereof or a dry powder.

  ADVANTAGE OF THE INVENTION According to this invention, the skin external preparation which exhibits an active ingredient with high safety derived from a natural product, and exhibits skin aging, rough skin improvement effect, and whitening effect (prevention and improvement of a spot, a buckwheat, a liver spot, etc.) is provided. be able to.

  Hereinafter, the present invention will be described in detail. The yeast used in the present invention is Saccharomyces mikatae belonging to the genus Saccharomyces, and examples thereof include Saccharomyces mikatae Naumov et al. It is known that this yeast can be collected from, for example, sap such as soil, camellia, shirakashi, beech and mizunara.

  Yeast extract, or a concentrate or dried product thereof can be prepared as follows. For example, the yeast extract production method includes a method of culturing yeast in a culture solution, a hydrolysis method in which the cultured yeast is solubilized with an acid or alkali, a self-degrading enzyme utilizing a proteolytic enzyme in the yeast cell, etc. It can be obtained by a digestion method, an enzyme method using an enzyme agent such as a proteolytic enzyme, or a method combining these.

  In addition, a yeast extract concentrate can be obtained by concentrating a yeast culture solution or a solution obtained by a hydrolysis method, a yeast self-digestion method or an enzyme method using a vacuum concentrator or the like.

  A dried yeast extract can be obtained by drying a yeast culture solution or a concentrated solution thereof by a vacuum drying method, a spray drying method, or the like.

  The yeast according to the present invention or a processed product thereof can be blended in a skin external preparation (cosmetics, quasi-drug, external medicine). Examples of skin external preparations include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, makeup press powders, blushers, white powders, facial cleansers, body shampoos, scalp, hair shampoos, hair conditioners. , Shampoos or tonics for hair growth, hair restoration, and cosmetics for cleaning such as soap, and bath preparations, and the like, but the present invention is not limited thereto.

  The blending amount of the yeast extract or the concentrate or dried product thereof according to the present invention is in the range of 0.002 to 5.0 wt% (solid content wt%, hereinafter the same) in the case of basic cosmetics as its solid content. In the case of makeup cosmetics, the range is 0.002 to 5.0% by weight. In the case of cosmetics for cleaning, the range is 0.002 to 20.0% by weight. In the case of hair cosmetics, the solid content is in the range of 0.0001 to 10.0% by weight.

  When the yeast extract according to the present invention or a concentrate or dried product thereof is used as an external preparation for skin, components that can be blended in the external preparation for skin, such as oil components, surfactants (synthetic systems, natural products), emulsifiers, moisturizing agents, etc. An agent, a thickener, an antiseptic / bactericidal agent, an antifoaming agent, a powder component, an antioxidant, a chelating agent, a pH adjusting agent, a pigment, a fragrance and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and the characteristic of the yeast extract which concerns on this invention, its concentrate, or a dried material are not impaired, it does not interfere with what is combined with another physiologically active ingredient at all.

  Here, as the oil component, for example, lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, coconut oil, chamomile oil, palm oil, cacao oil, meadow foam oil, bergamot Oils such as oil, rosehip oil, Arabian coffee tree seed oil, lambender oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, vanilla oil, plant-derived squalane; animals such as mink oil and turtle oil Fats and oils derived from: waxes such as beeswax, carnauba wax, rice wax, lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, squalane; myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid Fatty acids such as lauryl alcohol, cetanol, stearyl Synthetic esters such as isopropyl myristate, isopropyl palmitate, butyl oleate, cetyl isooctanoate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (eg, octyldodecyl stearate), synthetic triglycerides, etc. Can be mentioned.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyethylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene Nonionic surfactants such as oxyethylene hydrogenated castor oil, polyoxyethylene sorbitol fatty acid ester; fatty acid salt, alkyl sulfate, alkylbenzene sulfonate, polyoxyethylene alkyl ether sulfate, polyoxyethylene fatty amine sulfate, polyoxy Ethylene alkyl phenyl ether sulfate, polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene Anionic surfactants such as alkylphenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxys Cationic surfactants such as ethyl imidazolinium salt, N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, It is possible to use amphoteric surfactants such as N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine, etc. it can.

  In addition, as an emulsifier or an emulsifier, stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean Water-soluble polysaccharides, soy-derived protein-polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins And derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, 2-methacryloyloxyethyl phosphorylcholine / butyl methacrylate copolymer solution. Furthermore, saccharides such as trehalose, mucopolysaccharides (eg, hyaluronic acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, etc.), tuberose polysaccharides, elastin and derivatives thereof, collagen and derivatives thereof, NMF related Substance, hydrolyzed conchiolin, hydrolyzed silk, sphingomonas culture, sphingoglycolipid, ceramide, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Extract, various amino acids and derivatives thereof.

  Examples of the thickener include components derived from brown algae, green algae, or red algae such as alginic acid, agar, carrageenan, and fucoidan; silane root (white and white) extract; pectin, locust bean gum, aloe polysaccharide, alkaline-genes-producing polysaccharide Polysaccharides such as body; gums such as xanthan gum, tragacanth gum, roast bean gum, guar gum; cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose; polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, mastic resin, acrylic acid / methacrylic acid copolymer Synthetic polymers such as coalescence; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride , Benzalkonium chloride, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), polyphosphoric acid, propanediol, 1,2-pentanediol, various essential oils, dry bark, radish fermentation liquor, sugarcane Plant-derived ethanol or 1,3-butylene glycol.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, anhydrous silicic acid, mica, nylon powder, polyethylene powder, silk There are powders, cellulosic powders, powders of cereals (rice, wheat, corn, millet, etc.), powders of beans (soybeans, red beans, etc.), and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2- Hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract Etc.

  Examples of the antifoaming agent include ethanol, isopropanol, disiloxane, dimethylpolycyclosan, dimethicone silicate silica, trisiloxane, silylated silica, dimethicone, trimethylsiloxysilicate, and DPG isobornyl ether.

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, murasakixib extract, silane root extract, peony extract, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E-linoleate).

  Examples of the chelating agent include trisodium ethylenediaminehydroxyethyl triacetate, edetic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyethane diphosphonate, and the like.

  Examples of the pH adjuster include citric acid or a salt thereof, lactic acid or a salt thereof, glycolic acid, succinic acid, hydrochloric acid, monoethanolamine, diethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide, and the like.

  The whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof 4-methoxysalicylic acid potassium salt, magnolignan (5,5′-dipropyl-biphenyl-2,2′-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine mono) Phosphate, adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid glucoside and the like. Examples of ascorbic acid derivatives include derivatives such as L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid Ascorbic acid ester salts such as 2-sulfate magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleic acid, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbyl Acid, ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, acylated 6-positions of these ascorbic acid sugar derivatives (acyl group is hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate L-ascorbic acid tetrafatty acid esters such as L-ascorbic acid tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or Its acylated derivatives, glyceryl derivatives of ascorbic acid such as bisglyceryl ascorbic acid, aminopropyl L-ascorbate phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl asco Examples of hydroquinone derivatives include bilphosphate, arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives such as tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propioni. Luoxybenzoic acid and the like, nicotinic acid derivatives include nicotinic acid amide and nicotinic acid benzyl, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like. .

  Examples of the physiologically active component include placenta extract, Sahakuhi extract, Yukinosita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, and fermented white coconut Peony extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, pearl barley hydrolyzate, pearl barley seed fermented product, royal jelly fermented product, sake lees extract Alternatively, ceramide, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like contained therein. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe, etc. Seaweed extract of sea urchin, extract of marine flowering plant such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzate thereof, germinated rice fermented Product, black bean extract or hydrolyzate thereof, brown sugar extract or fermented product thereof, damask rose flower extract, ylang ylang flower extract, bamboo shoot extract, linoleic acid and its derivatives or processed products (for example, Liposomal linoleic acid, etc.), animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.), t-cycloamino acid derivatives, Tamine A and its derivatives, allantoin, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof, camellia extract, red ginseng extract , Honeycomb extract, Loofah extract, Anahaosa extract, Peach extract, Plum extract, Peach extract, Kiwi extract, Sunflower extract, Zizyphus joazeiro extract, Paudarco bark extract, Lily grass (Daily) Extract or fermented product, Hibiscus flower extract or fermented product, Hagoromogusa extract, Cherimoya extract, Mango extract, Mangosteen extract, Funori extract, Oolong tea extract, Benifu extract, Silane extract, Sweet potato extract , Yam pericarp or seed coat extract or hydrolyzate, safflower Extract, Casablanca extract, sweet potato extract or fermented product thereof, guava leaf extract, olive leaf extract, horse chestnut extract, ginger extract, vanilla extract, dokudami extract, evening birch extract, aloe extract Products, aloe vera extract, fig flower extract, apple extract, white asparagus extract and the like.

  Next, the present invention will be described more specifically with reference to production examples, test examples, and examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of yeast extract powder Yeast (Saccharomyces mikatae) was added to 100 mL of GP medium containing 1.0% glucose and 0.5% peptone that had been autoclaved in advance, followed by aeration and agitation culture at 30 ° C. A preculture was obtained. This preculture was further added to 900 mL of the same GP medium that had been prepared and autoclaved as described above, and cultured at 30 ° C. with aeration and stirring to obtain a yeast slurry. Purified water and 1N hydrochloric acid solution were added to the yeast slurry, and acid hydrolysis treatment was performed for 2 hours while stirring at 90 ° C. Thereafter, the treatment solution was filtered and the pH was adjusted to pH 5.5 with 1N sodium hydroxide solution to obtain a yeast culture acid hydrolyzate. This yeast culture acid hydrolyzate was concentrated and dried with a rotary evaporator to obtain 1.3 g of a dried yeast extract powder.

Production Example 2 Preparation of yeast extract powder Yeast (Saccharomyces mikatae) was added to 100 mL of GP medium containing 1.0% glucose and 0.5% peptone that had been autoclaved in advance, followed by aeration and agitation culture at 30 ° C. A preculture was obtained. This preculture was further added to a GP medium similar to that previously prepared and autoclaved, and cultured at 30 ° C. with aeration and stirring to obtain a yeast slurry. Sterile purified water and 1N hydrochloric acid solution were added to the yeast slurry, and self-digestion treatment was performed for 10 hours while stirring at 50 ° C. Thereafter, the treatment solution was filtered, and the pH was adjusted to 5.5 with a 1N sodium hydroxide solution to obtain a yeast autolysate. This yeast autolysate was concentrated and dried with a rotary evaporator to obtain a yeast extract dry powder.

Production Example 3 Preparation of yeast extract The yeast dry powder obtained in Production Example 1 was added to a mixed solvent of water and 1,3-butylene glycol and stirred, and then the filtrate obtained by filtration was used as a yeast extract.

Production Example 4 Preparation of yeast extract The yeast dry powder obtained in Production Example 2 was added to a mixed solvent of water and concentrated glycerin and stirred, and then the filtrate obtained by filtration was used as a yeast extract.

Formulation Example 1 Lotion [Ingredients] part Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Methylparaben 0.1
Yeast extract powder of Production Example 1 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Sodium citrate 0.2
Amount of purified water totaling 100 parts

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 2.0 parts of the yeast extract of Production Example 2 was used instead of the yeast extract of Production Example 1 contained in Formulation Example 1.

Formulation Example 3 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the yeast extract of Production Example 3 was used instead of the yeast extract of Production Example 1 contained in Formulation Example 1.

Formulation Example 4 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the yeast extract of Production Example 4 was used instead of the yeast extract of Production Example 1 included in Formulation Example 1.

Formulation Example 5 Emulsion [Ingredients] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Camellia oil 1.5
Polyoxyethylene (20) sorbitan monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soybean lecithin 1.5
Yeast extract of Production Example 1 3.0
Plum flower extract 1.0
Hydrolyzed conchiolin 0.5
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Xanthan gum 0.2
Sodium hyaluronate 0.01
Purified water Total amount of 100 parts Perfume Appropriate amount

Formulation Example 6 Emulsion In the ingredients of Formulation Example 5, an emulsion was obtained in the same manner as Formulation Example 5 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. It was.

Formulation Example 7 Emulsion In the same manner as in Formulation Example 5, except that 5.0 parts of nicotinamide is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide Got.

Formulation Example 8 Emulsion In the ingredients of Formulation Example 5, the emulsion was prepared in the same manner as in Formulation Example 5 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide were used instead of 2.0 parts of tranexamic acid. Obtained.

Formulation Example 9 Emulsion In the same manner as in Formulation Example 5, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide are used instead of 2.0 parts of tranexamic acid cetyl ester hydrochloride, the same as in Formulation Example 5. An emulsion was obtained.

Formulation Example 10 Emulsion [Ingredients] part squalane 3.0
Cetyl alcohol 1.0
Polyethylene glycol monostearate 0.2
Dipropylene glycol 1.0
Cetyl isooctanoate 1.0
Dimethylpolycyclohexane 1.0
Yeast extract of Production Example 2 2.0
Lactic acid bacteria fermented rice 2.0
Camellia flower extract 1.0
Seaweed extract 1.0
Raffinose 2.0
1,3-butylene glycol 2.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Acetylated sodium hyaluronate 0.2
Tuberose polysaccharide solution 2.0
Guar gum 3.0
Amount of purified water totaling 100 parts

Formulation Example 11 Emulsion In the ingredients of Formulation Example 10, an emulsion was obtained in the same manner as Formulation Example 10 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. It was.

Formulation Example 12. Emulsion In the same manner as in Formulation Example 8, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are used instead of 2.0 parts of L-ascorbic acid-2-glucoside. Got.

Formulation Example 13 Emulsion In the ingredients of Formulation Example 10, the emulsion was prepared in the same manner as Formulation Example 10 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide were used instead of 2.0 parts of potassium hydroxide. Obtained.

Formulation Example 14. Emulsion In the same manner as in Formulation Example 10, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide are used, 2.0 parts of tranexamic acid cetyl ester hydrochloride is used. An emulsion was obtained.

Formulation Example 15. Cream [ingredient] part olive oil 5.0
Jojoba oil 5.0
Squalane 5.0
Behenyl alcohol 2.0
Palmitic acid 2.5
Yeast extract of Production Example 1 2.0
Lactic acid bacteria fermented rice 2.0
Hydrogenated lecithin 0.5
Carboxyvinyl polymer 0.3
Sodium alginate 0.2
Seaweed extract 2.0
Methylparaben 0.15
Ethylparaben 0.03
Amount of purified water totaling 100 parts

Formulation Example 16. Cream [ingredient] part polyoxyethylene sorbitan monostearate 1.5
Cetyl alcohol 3.0
Glyceryl monostearate 1.0
Squalane 5.0
Olive oil 5.0
Dipropylene glycol 3.0
Yeast extract of Production Example 2 2.0
Camellia flower extract 1.0
Licorice extract 2.0
Citric acid 0.2
Methylparaben 0.2
Amount of purified water totaling 100 parts

Example 17. Liquid foundation [ingredient] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Yeast extract of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Titanium oxide 8.0
Talc 4.0
Coloring pigment Appropriate amount Purified water The total amount is 100 parts

Formulation Example 18. Body shampoo [ingredient] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Yeast extract of Production Example 4 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Formulation Example 19. Hair shampoo [ingredient] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Yeast extract of Production Example 1 2.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Example 20. Hair conditioner [ingredient] part polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.0
Stearyl alcohol 1.0
Methylparaben 0.1
Yeast extract of Production Example 2 2.0
1,3-butylene glycol 5.0
Amount of purified water totaling 100 parts

Test Example 1 Evaluation test of melanin synthesis inhibitory effect Cultured B16 mouse melanoma cells B16-F10 were seeded in a 24-well microplate at 2.4 × 10 ⁴ cells / hole and cultured in 10% FBS-containing RPMI 1640 medium at 37 ° C., 5.0% CO 2. after pre-incubated for 1 day under the conditions of _2, in 10% FBS-containing RPMI1640 medium 1.0% yeast extract of preparation 1, 2.0% concentration becomes thus prepared sample solution and the end A theophylline-containing medium adjusted to a concentration of 1 mM was added and cultured for 2 days under the same conditions. Next, the culture solution was removed, 200 μL of 1N NaOH / 10% dimethyl sulfoxide solution was added per well, sealed, and incubated at 50 ° C. for 2 hours to lyse the cells. 100 μL of this solution was transferred to another 96-well microplate, and the melanin value was measured at a wavelength of 490 nm using a microplate reader (Model 680, manufactured by Bio-Rad). On the other hand, 5 μL of the solution in which the same cells are lysed is transferred to another 96-well microplate, and 200 μL of Dye Reagent Concentrate (Bio-Rad) solution diluted 5-fold with purified water is added to the microplate reader (Model 680, Bio-Rad). The absorbance at a wavelength of 570 nm was measured. Separately, a known amount of bovine serum albumin (manufactured by Sigma) was serially diluted, and the amount of protein equivalent to albumin was measured from a calibration curve obtained in the same manner. The obtained absorbance was gradually reduced by the amount of protein to determine the amount of melanin per protein. In the case of no sample added with 2.0% PBS solution instead of the sample solution (control), the same operation as above was performed, and the amount of melanin per protein obtained here was determined per protein at the time of each sample addition. The relative value of the amount of melanin was determined and used as the melanin synthesis rate (%). For comparison, the same test was performed when 2 mM kojic acid was added instead of the sample solution (positive control).

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, it was confirmed that the yeast extract according to Production Examples 1 and 2 of the present invention has a melanin synthesis inhibitory effect. From this, it was confirmed that the yeast extract which concerns on the manufacture examples 1 and 2 of this invention is useful as an active ingredient of whitening and skin beautification.

Test Example 2 Epidermal cell proliferation promoting effect Human epidermal cells NHEK were seeded at 5 × 10 ³ cells / hole in a 96-well microplate containing HuMedia KG2 medium (manufactured by Kurabo Industries), under conditions of 37 ° C. and 5.0% CO ₂ . After pre-culturing for 1 day, HuMedia KG2 medium containing the sample solution prepared so that the yeast extract of Production Examples 1 and 2 is at a concentration of 1.0% and 2.0% is added, and the same conditions are further applied for 3 days. Cultured. After culturing for 3 days, the supernatant was discarded, washed once with PBS (−), added 100 μL / well of hoechst33342 reagent diluted 100-fold with PBS (−), incubated at 37 ° C. for 1 hour, and DNA was fluorescently stained. . Thereafter, fluorescence intensity (excitation: 355 nm, emission: 460 nm: fluorescence microplate reader [Fluoroscan Ascent, manufactured by Thermo Fisher Scientific)] was measured to determine the amount of DNA. For the group to which PBS (-) was added instead of the sample solution, the group where the same operation was performed was used as a control, and the relative value of the fluorescence intensity of each sample addition group to the fluorescence intensity (DNA amount) obtained here. Was determined as the cell growth rate (%).

The results of Test Example 2 are shown in Table 2.
[Table 2]
As shown in Table 2, it was confirmed that the yeast extract according to Production Examples 1 and 2 of the present invention has an effect of promoting epidermal cell proliferation. This suggests that the yeast extract according to Production Examples 1 and 2 of the present invention exhibits an effect of improving skin turnover. Moreover, in addition to the above-mentioned melanin synthesis inhibitory effect, the improvement of the skin turnover promotes the discharge of melanin, and also exhibits the effect of preventing and improving spots, freckles and liver spots.

Claims (2)

  An external preparation for skin containing, as an active ingredient, yeast (Saccharomyces mikatae) extract, or a concentrate or dry powder thereof.   An external preparation for skin whitening comprising a yeast (Saccharomyces mikatae) extract, a concentrate thereof or a dry powder as an active ingredient.