JP2018531013A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018531013A5 JP2018531013A5 JP2018518522A JP2018518522A JP2018531013A5 JP 2018531013 A5 JP2018531013 A5 JP 2018531013A5 JP 2018518522 A JP2018518522 A JP 2018518522A JP 2018518522 A JP2018518522 A JP 2018518522A JP 2018531013 A5 JP2018531013 A5 JP 2018531013A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- engineered nucleic
- acid construct
- acid encoding
- engineered
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000039446 nucleic acids Human genes 0.000 claims 29
- 108020004707 nucleic acids Proteins 0.000 claims 29
- 150000007523 nucleic acids Chemical class 0.000 claims 29
- 101710163270 Nuclease Proteins 0.000 claims 9
- 230000004568 DNA-binding Effects 0.000 claims 7
- 108091033409 CRISPR Proteins 0.000 claims 4
- 102000015694 estrogen receptors Human genes 0.000 claims 4
- 108010038795 estrogen receptors Proteins 0.000 claims 4
- 238000011144 upstream manufacturing Methods 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108020005004 Guide RNA Proteins 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 3
- 238000011830 transgenic mouse model Methods 0.000 claims 3
- 241000699666 Mus <mouse, genus> Species 0.000 claims 2
- 241000699660 Mus musculus Species 0.000 claims 2
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 2
- NKANXQFJJICGDU-QPLCGJKRSA-N Tamoxifen Chemical compound C=1C=CC=CC=1C(/CC)=C(C=1C=CC(OCCN(C)C)=CC=1)/C1=CC=CC=C1 NKANXQFJJICGDU-QPLCGJKRSA-N 0.000 claims 2
- 102000034356 gene-regulatory proteins Human genes 0.000 claims 2
- 108091006104 gene-regulatory proteins Proteins 0.000 claims 2
- 239000012212 insulator Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 101001000998 Homo sapiens Protein phosphatase 1 regulatory subunit 12C Proteins 0.000 claims 1
- 102100035620 Protein phosphatase 1 regulatory subunit 12C Human genes 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000004043 responsiveness Effects 0.000 claims 1
- 229960001603 tamoxifen Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562242884P | 2015-10-16 | 2015-10-16 | |
| US62/242,884 | 2015-10-16 | ||
| PCT/IB2016/001902 WO2017064566A2 (en) | 2015-10-16 | 2016-10-14 | Inducible modification of a cell genome |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2018531013A JP2018531013A (ja) | 2018-10-25 |
| JP2018531013A6 JP2018531013A6 (ja) | 2018-12-13 |
| JP2018531013A5 true JP2018531013A5 (https=) | 2019-11-28 |
| JP6936952B2 JP6936952B2 (ja) | 2021-09-22 |
Family
ID=58054378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018518522A Active JP6936952B2 (ja) | 2015-10-16 | 2016-10-14 | 細胞ゲノムの誘導性改変 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10947559B2 (https=) |
| EP (1) | EP3362561A2 (https=) |
| JP (1) | JP6936952B2 (https=) |
| CN (1) | CN108431225A (https=) |
| AU (1) | AU2016337408B2 (https=) |
| CA (1) | CA3001518A1 (https=) |
| WO (1) | WO2017064566A2 (https=) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| JP6936952B2 (ja) * | 2015-10-16 | 2021-09-22 | アストラゼネカ アクチボラグ | 細胞ゲノムの誘導性改変 |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| WO2017078631A1 (en) | 2015-11-05 | 2017-05-11 | Agency For Science, Technology And Research | Chemical-inducible genome engineering technology |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US12054733B2 (en) | 2017-06-16 | 2024-08-06 | Lonza Ltd | Universal self-regulating mammalian cell line platform for the production of biologics |
| KR101928659B1 (ko) * | 2017-06-19 | 2018-12-13 | 서울대학교병원 | Dna 변형 유도 단백질 복합체 및 이를 암호화하는 유전자를 포함하는 재조합 벡터 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020028533A1 (en) * | 2018-08-01 | 2020-02-06 | Yale University | Compositions and methods for identification of membrane targets for enhancement of t cell activity against cancer |
| CA3113325A1 (en) * | 2018-10-11 | 2020-04-16 | Eidgenossische Technische Hochschule Zurich | A method to treat disease using a nucleic acid vector encoding a highly compact multi-input logic gate |
| US11851663B2 (en) | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021111280A1 (en) * | 2019-12-04 | 2021-06-10 | Crispr Therapeutics Ag | Regulatable expression systems |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| CN111705080A (zh) * | 2020-05-14 | 2020-09-25 | 广州市第八人民医院 | Hbv非人动物模型的构建方法及其应用 |
| CN116262927B (zh) * | 2021-12-13 | 2024-04-26 | 中国科学院微生物研究所 | 基于CRISPR/Cas系统调控基因表达的方法及其应用 |
| WO2024231559A1 (en) | 2023-05-11 | 2024-11-14 | Astrazeneca Ab | Methods of treatment of chronic kidney disease |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| EP1761630A2 (en) | 2004-06-21 | 2007-03-14 | Novo Nordisk Health Care AG | Glycosylation-disrupted factor vii variants |
| WO2005123918A1 (ja) * | 2004-06-22 | 2005-12-29 | Tokai University Educational System | 外来遺伝子の誘導発現の制御が可能な発現ベクター |
| AU2013293270B2 (en) * | 2012-07-25 | 2018-08-16 | Massachusetts Institute Of Technology | Inducible DNA binding proteins and genome perturbation tools and applications thereof |
| EP2840140B2 (en) * | 2012-12-12 | 2023-02-22 | The Broad Institute, Inc. | Crispr-Cas based method for mutation of prokaryotic cells |
| JP6936952B2 (ja) * | 2015-10-16 | 2021-09-22 | アストラゼネカ アクチボラグ | 細胞ゲノムの誘導性改変 |
-
2016
- 2016-10-14 JP JP2018518522A patent/JP6936952B2/ja active Active
- 2016-10-14 US US15/768,482 patent/US10947559B2/en active Active
- 2016-10-14 EP EP16837979.0A patent/EP3362561A2/en active Pending
- 2016-10-14 CA CA3001518A patent/CA3001518A1/en active Pending
- 2016-10-14 CN CN201680074288.4A patent/CN108431225A/zh active Pending
- 2016-10-14 WO PCT/IB2016/001902 patent/WO2017064566A2/en not_active Ceased
- 2016-10-14 AU AU2016337408A patent/AU2016337408B2/en active Active
-
2021
- 2021-02-03 US US17/166,823 patent/US12509702B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018531013A5 (https=) | ||
| AU2025263805A1 (en) | Novel crispr enzymes and systems | |
| Lone et al. | CRISPR/Cas9 system: a bacterial tailor for genomic engineering | |
| Senís et al. | CRISPR/Cas9‐mediated genome engineering: an adeno‐associated viral (AAV) vector toolbox | |
| CN108690845B (zh) | 基因组编辑系统和方法 | |
| Piatek et al. | RNA‐guided transcriptional regulation in planta via synthetic dC as9‐based transcription factors | |
| JP6799586B2 (ja) | 遺伝子操作ＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ | |
| JP2021192624A (ja) | 特定のゲノム遺伝子座へのゲノムおよびエピゲノム調節タンパク質のｒｎａ誘導型標的化 | |
| US20170349905A1 (en) | Genome editing with split cas9 expressed from two vectors | |
| IL295858B1 (en) | gRNA molecules including TRACR and crRNA, compositions containing them and their use | |
| CN105886498A (zh) | CRISPR-Cas9特异性敲除人PCSK9基因的方法以及用于特异性靶向PCSK9基因的sgRNA | |
| JP2016500262A5 (https=) | ||
| HRP20201443T1 (hr) | Postupci i pripravci za liječenje genetskog stanja | |
| Bayoumi et al. | Potential use of CRISPR/Cas13 machinery in understanding virus–host interaction | |
| Nishizawa‐Yokoi et al. | A piggyBac‐mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice | |
| JP2022526646A5 (https=) | ||
| WO2013030176A3 (en) | Live attenuated influenza virus | |
| MX356630B (es) | Uso del promotor rd29 o fragmentos del mismo para expresión de transgenes inducible por estrés en algodón. | |
| Pflueger et al. | Harnessing targeted DNA methylation and demethylation using dCas9 | |
| Lu et al. | CRISPR/Cas9-mediated hitchhike expression of functional shRNAs at the porcine miR-17-92 cluster | |
| NZ608144A (en) | Sugarcane bacilliform viral (scbv) enhancer and its use in plant functional genomics | |
| Wang et al. | Complete genome sequence of a hemagglutination-negative avian paramyxovirus type 4 isolated from China | |
| Okamura et al. | The H19 imprinting control region mediates preimplantation imprinted methylation of nearby sequences in yeast artificial chromosome transgenic mice | |
| JP2024501892A (ja) | 新規の核酸誘導型ヌクレアーゼ | |
| Tang et al. | In vitro CRISPR-Cas9-mediated efficient Ad5 vector modification |