JP2018520706A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018520706A5 JP2018520706A5 JP2018522857A JP2018522857A JP2018520706A5 JP 2018520706 A5 JP2018520706 A5 JP 2018520706A5 JP 2018522857 A JP2018522857 A JP 2018522857A JP 2018522857 A JP2018522857 A JP 2018522857A JP 2018520706 A5 JP2018520706 A5 JP 2018520706A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- compartment
- library
- cartridge
- qpcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 32
- 108020004707 nucleic acids Proteins 0.000 claims 26
- 102000039446 nucleic acids Human genes 0.000 claims 26
- 238000011529 RT qPCR Methods 0.000 claims 12
- 239000012472 biological sample Substances 0.000 claims 9
- 239000000523 sample Substances 0.000 claims 9
- 238000000034 method Methods 0.000 claims 7
- 238000007481 next generation sequencing Methods 0.000 claims 7
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 238000003908 quality control method Methods 0.000 claims 4
- 238000005382 thermal cycling Methods 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 108091093088 Amplicon Proteins 0.000 claims 1
- 238000004891 communication Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15178158.0 | 2015-07-23 | ||
| EP15178158 | 2015-07-23 | ||
| PCT/EP2016/067149 WO2017013103A1 (en) | 2015-07-23 | 2016-07-19 | Automated sample to ngs library preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018520706A JP2018520706A (ja) | 2018-08-02 |
| JP2018520706A5 true JP2018520706A5 (enExample) | 2019-08-15 |
Family
ID=53785468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018522857A Withdrawn JP2018520706A (ja) | 2015-07-23 | 2016-07-19 | 試料採取からngsライブラリ調製までの自動化 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20180243719A1 (enExample) |
| EP (1) | EP3325152B1 (enExample) |
| JP (1) | JP2018520706A (enExample) |
| CN (1) | CN108136389A (enExample) |
| CA (1) | CA2991267A1 (enExample) |
| WO (1) | WO2017013103A1 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018522588A (ja) | 2015-07-23 | 2018-08-16 | バイオカルティス エン フェー | 最適化された臨床試料のシーケンシング |
| US12492430B2 (en) * | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| CA3044782A1 (en) | 2017-12-29 | 2019-06-29 | Clear Labs, Inc. | Automated priming and library loading device |
| WO2021236328A1 (en) * | 2020-05-22 | 2021-11-25 | Novartis Ag | Cdna library generation |
| KR20230098259A (ko) * | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| CN112725905A (zh) * | 2020-12-31 | 2021-04-30 | 张研 | 一种数字微流控文库构建系统 |
| WO2024102996A1 (en) * | 2022-11-10 | 2024-05-16 | National Resilience, Inc. | Systems, methods, and compositions for generation of therapeutic cells |
| CN117309825B (zh) * | 2023-11-30 | 2024-04-09 | 四川蜀道建筑科技有限公司 | 一种透光检测亚甲蓝mb值的设备 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050208539A1 (en) * | 2003-12-31 | 2005-09-22 | Vann Charles S | Quantitative amplification and detection of small numbers of target polynucleotides |
| EP2315848B1 (en) * | 2008-07-18 | 2014-12-10 | Canon U.S. Life Sciences, Inc. | Methods and systems for microfluidic dna sample preparation |
| CN103003449A (zh) * | 2010-05-06 | 2013-03-27 | 艾比斯生物科学公司 | 集成样品制备系统和稳定的酶混合物 |
| WO2012012779A2 (en) * | 2010-07-23 | 2012-01-26 | Beckman Coulter Inc. | System and method including analytical units |
| WO2014199336A2 (en) * | 2013-06-13 | 2014-12-18 | Vela Operations Pte.Ltd. | Improved ngs workflow |
| US9498778B2 (en) * | 2014-11-11 | 2016-11-22 | Genmark Diagnostics, Inc. | Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system |
-
2016
- 2016-07-19 EP EP16744343.1A patent/EP3325152B1/en active Active
- 2016-07-19 WO PCT/EP2016/067149 patent/WO2017013103A1/en not_active Ceased
- 2016-07-19 CN CN201680042820.4A patent/CN108136389A/zh active Pending
- 2016-07-19 JP JP2018522857A patent/JP2018520706A/ja not_active Withdrawn
- 2016-07-19 CA CA2991267A patent/CA2991267A1/en not_active Abandoned
- 2016-07-19 US US15/578,161 patent/US20180243719A1/en not_active Abandoned
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018520706A5 (enExample) | ||
| Takahashi et al. | 5′ end–centered expression profiling using cap-analysis gene expression and next-generation sequencing | |
| EP4234717A3 (en) | High throughput multiomics sample analysis | |
| JP2016525344A5 (enExample) | ||
| JP2015156872A5 (enExample) | ||
| JP2019501641A (ja) | ナノポア技術を用いた短いdna断片の迅速な配列決定 | |
| Carroll et al. | RNA-sequencing of Staphylococcus aureus messenger RNA | |
| US20240287596A1 (en) | Method for sequencing rna oligonucleotides | |
| JP2013543370A5 (enExample) | ||
| Matsumura et al. | SuperSAGE: powerful serial analysis of gene expression | |
| JP2016520326A (ja) | マルチプレックス配列決定のための分子バーコード化 | |
| JP2019536441A5 (enExample) | ||
| JP2017521086A5 (enExample) | ||
| CN119790151A (zh) | 制备标准化核酸样品的方法、用于该方法的试剂盒和装置 | |
| Benjak et al. | Whole-transcriptome sequencing for high-resolution transcriptomic analysis in Mycobacterium tuberculosis | |
| EP3099812B1 (en) | Method and kit with internal amplification control | |
| Lin et al. | Mod-seq: a high-throughput method for probing RNA secondary structure | |
| Maatz et al. | Transcriptome‐wide Identification of RNA‐binding protein binding sites using photoactivatable‐ribonucleoside‐enhanced crosslinking immunoprecipitation (PAR‐CLIP) | |
| EP3191603B1 (en) | Method of obtaining paired-end sequencing information | |
| Spealman et al. | Exploring ribosome positioning on translating transcripts with ribosome profiling | |
| Matteau et al. | Precise identification of genome-wide transcription start sites in bacteria by 5′-rapid amplification of cDNA ends (5′-RACE) | |
| EP3749779B1 (en) | Library preparation | |
| WO2012083845A1 (zh) | 用于除去测序文库中载体片段的方法及其用途 | |
| Shell et al. | RNA sequencing for transcript 5′-end mapping in mycobacteria | |
| CN115478098A (zh) | 一种单细胞转录组及染色质可及性双组学测序文库构建方法及测序方法 |