JP2018515103A - ポリメラーゼバリアントおよびその使用 - Google Patents

ポリメラーゼバリアントおよびその使用 Download PDF

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Publication number
JP2018515103A
JP2018515103A JP2017559318A JP2017559318A JP2018515103A JP 2018515103 A JP2018515103 A JP 2018515103A JP 2017559318 A JP2017559318 A JP 2017559318A JP 2017559318 A JP2017559318 A JP 2017559318A JP 2018515103 A JP2018515103 A JP 2018515103A
Authority
JP
Japan
Prior art keywords
polymerase
modified
nucleotide
dna polymerase
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017559318A
Other languages
English (en)
Japanese (ja)
Inventor
エイヤー，アルナ
シュワブ，チャールズ
アーノルド，クレオマ
レダーマン，イリア
タイ，アイリーン
シュルツ，タイラー・オブライエン
マクガー，コリン・アレクサンダー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/012,317 external-priority patent/US10308918B2/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2018515103A publication Critical patent/JP2018515103A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017559318A 2015-05-14 2016-05-13 ポリメラーゼバリアントおよびその使用 Pending JP2018515103A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201562161571P 2015-05-14 2015-05-14
US62/161,571 2015-05-14
US201562202895P 2015-08-09 2015-08-09
US62/202,895 2015-08-09
US15/012,317 2016-02-01
US15/012,317 US10308918B2 (en) 2015-02-02 2016-02-01 Polymerase variants
US15/151,364 US10526588B2 (en) 2015-05-14 2016-05-10 Polymerase variants and uses thereof
US15/151,364 2016-05-10
PCT/US2016/032258 WO2016183403A2 (en) 2015-05-14 2016-05-13 Polymerase variants and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019104273A Division JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用

Publications (1)

Publication Number Publication Date
JP2018515103A true JP2018515103A (ja) 2018-06-14

Family

ID=57248698

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017559318A Pending JP2018515103A (ja) 2015-05-14 2016-05-13 ポリメラーゼバリアントおよびその使用
JP2019104273A Pending JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用
JP2021086806A Pending JP2021121221A (ja) 2015-05-14 2021-05-24 ポリメラーゼバリアントおよびその使用

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2019104273A Pending JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用
JP2021086806A Pending JP2021121221A (ja) 2015-05-14 2021-05-24 ポリメラーゼバリアントおよびその使用

Country Status (6)

Country Link
US (3) US10526588B2 (enExample)
EP (1) EP3294749A4 (enExample)
JP (3) JP2018515103A (enExample)
CN (1) CN107636004A (enExample)
CA (2) CA3028797C (enExample)
WO (1) WO2016183403A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019512217A (ja) * 2016-02-29 2019-05-16 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
EP3377648B1 (en) 2015-11-16 2021-01-06 Stratos Genomics, Inc. Dp04 polymerase variants
US10590480B2 (en) 2016-02-29 2020-03-17 Roche Sequencing Solutions, Inc. Polymerase variants
JP6690005B2 (ja) * 2016-02-29 2020-04-28 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアシーケンシングのためのポリメラーゼ−鋳型複合体
WO2017202917A1 (en) 2016-05-27 2017-11-30 F. Hoffmann-La Roche Ag Tagged multi-nucleotides useful for nucleic acid sequencing
US10669580B2 (en) 2016-08-26 2020-06-02 Roche Sequencing Solutions, Inc. Tagged nucleotides useful for nanopore detection
JP7157048B2 (ja) 2016-09-22 2022-10-19 エフ．ホフマン－ラ ロシュ アーゲー Ｐｏｌ６ポリメラーゼバリアント
ES2987024T3 (es) 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
US11530392B2 (en) 2017-12-11 2022-12-20 Stratos Genomics, Inc. DPO4 polymerase variants with improved accuracy
WO2019166457A1 (en) 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Tagged nucleoside compounds useful for nanopore detection
CN112041331B (zh) 2018-02-28 2024-05-28 豪夫迈·罗氏有限公司 α-溶血素变体及其用途
JP7592731B2 (ja) 2020-02-06 2024-12-02 エフ． ホフマン－ラ ロシュ アーゲー ナノポアへの鋳型の貫通を低減する組成物
ES2999861T3 (en) 2020-08-11 2025-02-27 Hoffmann La Roche Nucleoside-5'-oligophosphates tagged with positively-charged polymers, nanopores incorporating negative charges, and methods and systems using the same
CN118056019A (zh) 2021-06-17 2024-05-17 豪夫迈·罗氏有限公司 具有穿过通道的带负电荷的聚合物的工程化纳米孔
WO2022263489A1 (en) 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
EP4373925A1 (en) 2021-07-21 2024-05-29 F. Hoffmann-La Roche AG Alpha-hemolysin variants forming narrow channel pores and uses thereof
EP4437090A1 (en) 2021-11-22 2024-10-02 F. Hoffmann-La Roche AG Thermostable polymerase variants

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US6346379B1 (en) 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0509508D0 (en) 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
CN102046291B (zh) 2008-04-04 2014-07-16 It-Is国际有限公司 用于化学和生化反应的热控系统和方法
ES2742800T3 (es) * 2008-11-03 2020-02-17 Kapa Biosystems Inc ADN polimerasas de tipo A modificadas
CN103298984B (zh) 2010-02-08 2015-11-25 吉尼亚科技公司 用于在纳米孔中操作分子的系统和方法
GB201002362D0 (en) 2010-02-11 2010-03-31 Isis Innovation Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
US8936911B2 (en) 2010-09-22 2015-01-20 Pacific Biosciences Of California, Inc. Purified extended polymerase/template complex for sequencing
EP2652153B1 (en) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2012173905A1 (en) 2011-06-16 2012-12-20 The Regents Of The Unversity Of California Salt-tolerant dna polymerases
EP2742151B1 (en) 2011-08-10 2017-10-25 Life Technologies Corporation Polymerase compositions
GB2510719A (en) 2012-06-15 2014-08-13 Genia Technologies Inc Chip set-up and high-accuracy nucleic acid sequencing
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
JP2016171215A (ja) 2015-03-12 2016-09-23 株式会社東芝 半導体記憶装置
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
ES2880331T3 (es) 2016-02-29 2021-11-24 Genia Tech Inc Variantes de polimerasa
JP6959931B2 (ja) 2016-02-29 2021-11-05 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PLOS ONE, vol. Vol.7, Issue 5, JPN6018051901, 2012, pages 38283, ISSN: 0003952270 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019512217A (ja) * 2016-02-29 2019-05-16 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Also Published As

Publication number Publication date
JP2019187424A (ja) 2019-10-31
JP2021121221A (ja) 2021-08-26
US20160333327A1 (en) 2016-11-17
US20200087636A1 (en) 2020-03-19
CA2978194A1 (en) 2016-11-17
CA3028797C (en) 2023-01-03
WO2016183403A2 (en) 2016-11-17
US11788073B2 (en) 2023-10-17
US10526588B2 (en) 2020-01-07
CA3028797A1 (en) 2016-11-17
CN107636004A (zh) 2018-01-26
WO2016183403A3 (en) 2017-01-05
EP3294749A4 (en) 2018-10-31
US20210189355A1 (en) 2021-06-24
CA2978194C (en) 2021-11-16
US10968438B2 (en) 2021-04-06
EP3294749A2 (en) 2018-03-21

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