JP2018515103A - ポリメラーゼバリアントおよびその使用 - Google Patents

ポリメラーゼバリアントおよびその使用 Download PDF

Info

Publication number
JP2018515103A
JP2018515103A JP2017559318A JP2017559318A JP2018515103A JP 2018515103 A JP2018515103 A JP 2018515103A JP 2017559318 A JP2017559318 A JP 2017559318A JP 2017559318 A JP2017559318 A JP 2017559318A JP 2018515103 A JP2018515103 A JP 2018515103A
Authority
JP
Japan
Prior art keywords
polymerase
modified
nucleotide
dna polymerase
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017559318A
Other languages
English (en)
Japanese (ja)
Inventor
エイヤー，アルナ
シュワブ，チャールズ
アーノルド，クレオマ
レダーマン，イリア
タイ，アイリーン
シュルツ，タイラー・オブライエン
マクガー，コリン・アレクサンダー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/012,317 external-priority patent/US10308918B2/en
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2018515103A publication Critical patent/JP2018515103A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2017559318A 2015-05-14 2016-05-13 ポリメラーゼバリアントおよびその使用 Pending JP2018515103A (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201562161571P 2015-05-14 2015-05-14
US62/161,571 2015-05-14
US201562202895P 2015-08-09 2015-08-09
US62/202,895 2015-08-09
US15/012,317 US10308918B2 (en) 2015-02-02 2016-02-01 Polymerase variants
US15/012,317 2016-02-01
US15/151,364 US10526588B2 (en) 2015-05-14 2016-05-10 Polymerase variants and uses thereof
US15/151,364 2016-05-10
PCT/US2016/032258 WO2016183403A2 (en) 2015-05-14 2016-05-13 Polymerase variants and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2019104273A Division JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用

Publications (1)

Publication Number Publication Date
JP2018515103A true JP2018515103A (ja) 2018-06-14

Family

ID=57248698

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2017559318A Pending JP2018515103A (ja) 2015-05-14 2016-05-13 ポリメラーゼバリアントおよびその使用
JP2019104273A Pending JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用
JP2021086806A Pending JP2021121221A (ja) 2015-05-14 2021-05-24 ポリメラーゼバリアントおよびその使用

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2019104273A Pending JP2019187424A (ja) 2015-05-14 2019-06-04 ポリメラーゼバリアントおよびその使用
JP2021086806A Pending JP2021121221A (ja) 2015-05-14 2021-05-24 ポリメラーゼバリアントおよびその使用

Country Status (6)

Country Link
US (3) US10526588B2 (enExample)
EP (1) EP3294749A4 (enExample)
JP (3) JP2018515103A (enExample)
CN (1) CN107636004A (enExample)
CA (2) CA3028797C (enExample)
WO (1) WO2016183403A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019512217A (ja) * 2016-02-29 2019-05-16 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107257854B (zh) * 2015-02-02 2022-02-15 豪夫迈·罗氏有限公司 聚合酶变体
US10526588B2 (en) * 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
ES2846949T3 (es) 2015-11-16 2021-07-30 Stratos Genomics Inc Variantes de la polimerasa DPO4
US20170268052A1 (en) * 2016-02-29 2017-09-21 Genia Technologies, Inc. Polymerase-template complexes
US10590480B2 (en) 2016-02-29 2020-03-17 Roche Sequencing Solutions, Inc. Polymerase variants
US10655174B2 (en) 2016-05-27 2020-05-19 Roche Sequencing Solutions, Inc. Tagged multi-nucleotides useful for nucleic acid sequencing
US10669580B2 (en) 2016-08-26 2020-06-02 Roche Sequencing Solutions, Inc. Tagged nucleotides useful for nanopore detection
CN110114458A (zh) 2016-09-22 2019-08-09 豪夫迈·罗氏有限公司 Pol6聚合酶变体
WO2018204707A1 (en) 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
AU2018383610B2 (en) * 2017-12-11 2024-09-12 F. Hoffmann-La Roche Ag DPO4 polymerase variants with improved accuracy
EP3759116A1 (en) 2018-02-28 2021-01-06 F. Hoffmann-La Roche AG Tagged nucleoside compounds useful for nanopore detection
WO2019166458A1 (en) 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Alpha-hemolysin variants and uses thereof
EP4100415A1 (en) 2020-02-06 2022-12-14 F. Hoffmann-La Roche AG Compositions that reduce template threading into a nanopore
US20240150824A1 (en) 2020-08-11 2024-05-09 Roche Sequencing Solutions, Inc. Nucleoside-5'-oligophosphates tagged with postiviely-charged polymers, nanopores incorporating negative charges, and methods and systems using the same
WO2022263489A1 (en) 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Nucleoside-5 -oligophosphates having a cationically-modified nucleobase
WO2022263496A1 (en) 2021-06-17 2022-12-22 F. Hoffmann-La Roche Ag Engineered nanopore with a negatively charged polymer threaded through the channel
US20250084471A1 (en) 2021-07-21 2025-03-13 Roche Sequencing Solutions, Inc. Alpha-hemolysin variants forming narrow channel pores and uses thereof
WO2023089175A1 (en) 2021-11-22 2023-05-25 F. Hoffmann-La Roche Ag Thermostable polymerase variants

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US6346379B1 (en) 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
GB0509508D0 (en) 2005-05-10 2005-06-15 Solexa Ltd Improved polymerases
CA2720483A1 (en) 2008-04-04 2009-10-08 It-Is International Ltd. Thermal control system and method for chemical and biochemical reactions
EP3101124B1 (en) * 2008-11-03 2019-06-19 Kapa Biosystems, Inc. Modified type a dna polymerases
EP2843405B8 (en) 2010-02-08 2023-10-04 Roche Sequencing Solutions, Inc. Systems and methods for manipulating a molecule in a nanopore
GB201002362D0 (en) 2010-02-11 2010-03-31 Isis Innovation Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds
US8936911B2 (en) 2010-09-22 2015-01-20 Pacific Biosciences Of California, Inc. Purified extended polymerase/template complex for sequencing
US10443096B2 (en) 2010-12-17 2019-10-15 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using modified nucleotides and nanopore detection
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2012173905A1 (en) 2011-06-16 2012-12-20 The Regents Of The Unversity Of California Salt-tolerant dna polymerases
LT2742151T (lt) 2011-08-10 2018-02-12 Life Technologies Corporation Polimerazės kompozicija
GB2510719A (en) 2012-06-15 2014-08-13 Genia Technologies Inc Chip set-up and high-accuracy nucleic acid sequencing
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
JP2016171215A (ja) 2015-03-12 2016-09-23 株式会社東芝 半導体記憶装置
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
ES2880331T3 (es) 2016-02-29 2021-11-24 Genia Tech Inc Variantes de polimerasa
JP6959931B2 (ja) 2016-02-29 2021-11-05 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016124543A1 (en) * 2015-02-02 2016-08-11 F. Hoffmann-La Roche Ag Polymerase variants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PLOS ONE, vol. Vol.7, Issue 5, JPN6018051901, 2012, pages 38283, ISSN: 0003952270 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019512217A (ja) * 2016-02-29 2019-05-16 ジェニア・テクノロジーズ・インコーポレイテッド エキソヌクレアーゼ欠損ポリメラーゼ

Also Published As

Publication number Publication date
CA3028797C (en) 2023-01-03
EP3294749A4 (en) 2018-10-31
JP2021121221A (ja) 2021-08-26
CA2978194A1 (en) 2016-11-17
JP2019187424A (ja) 2019-10-31
CN107636004A (zh) 2018-01-26
WO2016183403A3 (en) 2017-01-05
WO2016183403A2 (en) 2016-11-17
US20200087636A1 (en) 2020-03-19
CA2978194C (en) 2021-11-16
US20160333327A1 (en) 2016-11-17
US20210189355A1 (en) 2021-06-24
US10968438B2 (en) 2021-04-06
EP3294749A2 (en) 2018-03-21
US11788073B2 (en) 2023-10-17
CA3028797A1 (en) 2016-11-17
US10526588B2 (en) 2020-01-07

Similar Documents

Publication Publication Date Title
US10961520B2 (en) Polymerase variants
US11788073B2 (en) Polymerase variants and uses thereof
US11718836B2 (en) Exonuclease deficient polymerases
JP7157048B2 (ja) Ｐｏｌ６ポリメラーゼバリアント

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20171127

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190107

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20190405

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190604

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20191111