JP2018502575A5 - - Google Patents

Info

Publication number

JP2018502575A5

JP2018502575A5 JP2017535832A JP2017535832A JP2018502575A5 JP 2018502575 A5 JP2018502575 A5 JP 2018502575A5 JP 2017535832 A JP2017535832 A JP 2017535832A JP 2017535832 A JP2017535832 A JP 2017535832A JP 2018502575 A5 JP2018502575 A5 JP 2018502575A5

Authority

JP

Japan

Prior art keywords

cells

tgfβ

superfamily

cell

medium

Prior art date

2015-12-30

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims description 54
• 210000004027 cell Anatomy 0.000 claims description 42
• DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical group NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims description 36
• 102000004887 Transforming Growth Factor beta Human genes 0.000 claims description 31
• 108090001012 Transforming Growth Factor beta Proteins 0.000 claims description 31
• 229960003966 nicotinamide Drugs 0.000 claims description 18
• 235000005152 nicotinamide Nutrition 0.000 claims description 18
• 239000011570 nicotinamide Substances 0.000 claims description 18
• 230000004069 differentiation Effects 0.000 claims description 15
• 210000001671 embryonic stem cell Anatomy 0.000 claims description 14
• 239000003795 chemical substances by application Substances 0.000 claims description 11
• 238000012258 culturing Methods 0.000 claims description 11
• 229920001184 polypeptide Polymers 0.000 claims description 10
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 10
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 10
• ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 claims description 10
• 102100033356 Lecithin retinol acyltransferase Human genes 0.000 claims description 8
• 102100028001 Retinaldehyde-binding protein 1 Human genes 0.000 claims description 8
• 101710101931 Retinaldehyde-binding protein 1 Proteins 0.000 claims description 8
• 108010084957 lecithin-retinol acyltransferase Proteins 0.000 claims description 8
• 210000001778 pluripotent stem cell Anatomy 0.000 claims description 8
• 239000000126 substance Substances 0.000 claims description 7
• 208000002111 Eye Abnormalities Diseases 0.000 claims description 6
• 102100035846 Pigment epithelium-derived factor Human genes 0.000 claims description 6
• 208000030533 eye disease Diseases 0.000 claims description 6
• 108090000102 pigment epithelium-derived factor Proteins 0.000 claims description 6
• GSCPDZHWVNUUFI-UHFFFAOYSA-N 3-aminobenzamide Chemical compound NC(=O)C1=CC=CC(N)=C1 GSCPDZHWVNUUFI-UHFFFAOYSA-N 0.000 claims description 4
• 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 4
• 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 4
• 108010023082 activin A Proteins 0.000 claims description 4
• 230000004186 co-expression Effects 0.000 claims description 4
• 239000003814 drug Substances 0.000 claims description 4
• 210000002950 fibroblast Anatomy 0.000 claims description 4
• 235000020945 retinal Nutrition 0.000 claims description 4
• 239000011604 retinal Substances 0.000 claims description 4
• 229940124597 therapeutic agent Drugs 0.000 claims description 4
• 230000001225 therapeutic effect Effects 0.000 claims description 4
• NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 claims description 4
• 102100022430 Melanocyte protein PMEL Human genes 0.000 claims description 2
• 101710130208 Melanocyte protein PMEL Proteins 0.000 claims description 2
• NCYCYZXNIZJOKI-OVSJKPMPSA-N Retinaldehyde Chemical compound O=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-OVSJKPMPSA-N 0.000 claims description 2
• 230000005856 abnormality Effects 0.000 claims description 2
• 230000001464 adherent effect Effects 0.000 claims description 2
• 102000023732 binding proteins Human genes 0.000 claims description 2
• 108091008324 binding proteins Proteins 0.000 claims description 2
• 210000002919 epithelial cell Anatomy 0.000 claims description 2
• 210000001316 polygonal cell Anatomy 0.000 claims description 2
• 102000004169 proteins and genes Human genes 0.000 claims description 2
• 108090000623 proteins and genes Proteins 0.000 claims description 2
• 230000002207 retinal effect Effects 0.000 claims description 2
• 230000028327 secretion Effects 0.000 claims description 2
• 102000009618 Transforming Growth Factors Human genes 0.000 claims 1
• 108010009583 Transforming Growth Factors Proteins 0.000 claims 1
• 239000000790 retinal pigment Substances 0.000 claims 1
• 239000000463 material Substances 0.000 description 3
• 238000012744 immunostaining Methods 0.000 description 1