JP2018193335A - Skin external preparation - Google Patents

Abstract

To provide a skin external preparation and a hair restorer that have an effect of preventing and improving the pigmentation of skin (including scalp), an effect of improving a blood flow, an anti-aging effect, and a hair growth effect, and have reduced skin irritation and excellent biological safety.SOLUTION: The present invention provides a skin external preparation and a hair restorer containing hydrolysates of plants of Campanulaceae, Codonopsis or extract thereof as an active ingredient.SELECTED DRAWING: None

Description

  The present invention can be added to a skin external preparation or a hair restoration agent, and has effects of preventing and improving skin pigmentation (including scalp), blood flow improvement effect, anti-aging effect and hair growth effect, and skin ( The present invention relates to a novel active ingredient that is less irritating to the scalp (including the scalp) and has excellent biological safety.

  In recent years, research has been conducted on cell aging and external factors (for example, ultraviolet rays, chemical substances such as air pollutants and environmental hormones, allergens such as pollen, environmental stress, etc.), and various cells have been studied. The mechanism of aging and cell damage and repair have been elucidated.

  For example, regarding skin cells, inactivation of cell growth / differentiation associated with aging, decrease in hormone secretion, quantitative decrease in extracellular matrix components (collagen, laminin, etc.) constituting the skin, or internal circulation such as poor circulation Factors and active factors induced by sunlight (ultraviolet rays), chemical substances such as air pollutants and environmental hormones, allergens such as pollen, and external factors such as environmental stress are intricately intertwined to cause aging (wrinkle) , Tarmi, etc.), rough skin, and changes in color tone are known to occur.

  Furthermore, ultraviolet rays, chemical substances, and allergens, which are external factors, damage cells and tissues in the living body to alter biological components or generate active oxygen. Thereby, an antigen is generated in the cell and inflammation occurs in the living body. Furthermore, the above external factors induce abnormal deposition of intracellular melanin pigments, causing spots, freckles, liver spots and the like on the skin.

  Various active ingredients have been proposed for the purpose of preventing and improving the unhealthy and aging of cells as described above, and cosmetics, foods and drinks and pharmaceuticals containing these active ingredients have been put on the market. For example, antioxidants such as vitamin C, vitamin E, and catalase; anti-inflammatory agents such as glycyrrhizic acid or salts thereof, allantoin, tranexamic acid; various ultraviolet absorbers; α-hydroxycarboxylic acid, placenta extract, γ-amino- Cell activation components such as β-hydroxybutyric acid; extracellular matrix components such as collagen, elastin, hyaluronic acid or salts thereof; humectants such as urea; and protein glycation inhibitors such as aminoguanidine. In addition, kojic acid, linoleic acid, and the like are known as substances that suppress the occurrence of pigmentation such as skin spots, buckwheat, and liver spots, and are widely used as active ingredients of whitening agents.

  In view of the problems of the prior art, the present inventors have conducted intensive research to find new active ingredients derived from natural products. As a result, the hydrolyzate of the plant belonging to the genus Zucarinaceae or its extract has an excellent inhibitory effect on pigmentation (stains, buckwheat, liver spots, etc.) caused by ultraviolet rays, and further improves blood flow. A skin external preparation that has excellent skin-healing effect, anti-aging effect, whitening effect, and hair-growth effect by blending the hydrolyzate. It was also found that it is possible to provide a hair restorer.

Conventionally, it has been disclosed in, for example, Patent Document 1 that an extract of a plant belonging to the genus Zucarinaceae has a whitening effect. However, the hydrolyzate of these plants or the extract thereof is pigmented by ultraviolet rays. It has not been known about having a suppressive effect and a blood flow improving effect.
JP 2005-145888

The present invention is an external preparation for skin containing as an active ingredient a hydrolyzate of a plant belonging to the genus Zucarinaceae, or its extract.
The present invention is a hair restorer comprising as an active ingredient a hydrolyzate of a plant belonging to the genus Ceraminaceae, or an extract thereof.

  The present invention is a skin external preparation and hair restorer comprising as an active ingredient a hydrolyzate of a plant belonging to the genus Zucarinaceae, or an extract thereof, and according to the present invention, an extract that is an active ingredient is blended. Further, it is possible to provide an external preparation for skin and a hair-restoring agent that exert a skin-smoothing effect, including a scalp, a whitening effect, and a hair-restoring effect.

Hereinafter, preferred embodiments of the present invention will be described in detail.
In the present invention,

  The plant used in the present invention belongs to the genus Campanulaceae (Codonopsis). ) And Tousan (Codonopsis tangshen).

  The extract should be prepared by washing the target areas (whole grass, roots, flowers, leaves, stems, etc.) of the genus Cultivation in advance with water if necessary to remove foreign substances, and then directly or after drying. It can be performed by chopping or pulverizing and bringing into contact with an extraction solvent according to a conventional method such as dipping or countercurrent extraction.

  As an extraction solvent, water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-propanediol, 1,3-butylene glycol and glycerol; ethyl acetate and acetic acid Examples include esters such as butyl and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform. Alternatively, two or more kinds are used in combination.

  In the case of using a mixed solvent, the mixing ratio is, for example, a mixed solvent of water and 1,3-butylene glycol or 1,3-propanediol. In the case of a mixed solvent of ethanol and ethanol, it is preferably in the range of 1:10 to 25: 1, and in the case of a mixed solvent of water and glycerin, the range is 1: 1 to 20: 1.

  Moreover, the weight ratio of the dry part of each plant to the extraction solvent is preferably in the range of 1: 1 to 1:80, and more preferably in the range of 1: 5 to 1:50.

  In preparing the extract, the pH is not particularly limited, but it is generally preferably in the range of 3-9. In this sense, if necessary, the extraction solvent is blended with an alkaline adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide, or an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid or sulfuric acid. You may adjust so that it may become pH of.

  Extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, water or alcohols (polyhydric alcohol, lower alcohol, etc.), or a mixture of water and alcohols is used as the solvent. In some cases, the extraction temperature is generally in the range of 0 ° C. to 90 ° C., and the extraction time is generally in the range of 0.5 hour to 7 days.

  In the present invention, the extract obtained by the above extraction treatment is subjected to a hydrolysis treatment before or after extraction or in parallel with the extraction. Examples of the hydrolysis treatment include a method using an acid, an alkali, an enzyme, and the like, but a hydrolysis treatment using an enzyme is preferable. In the case where hydrolysis is performed using an enzyme, and when a solvent other than water or a mixed solvent of water and a hydrophilic solvent is used for preparing the extract solution, the extract solvent is once removed from the extract solution, The extract obtained here is preferably subjected to an enzymatic decomposition treatment after redissolving in water or a mixed solvent of water and a hydrophilic solvent.

  Examples of enzymes used for the hydrolysis treatment include proteolytic enzymes, starch degrading enzymes, fibrinolytic enzymes, and lipolytic enzymes. These enzymes may be used alone or in combination with a plurality of enzymes depending on the plant species.

  Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase, Bromelain, microorganism-derived complex proteolytic enzyme [for example, neurase (manufactured by Amano Enzyme Co., Ltd.)] and the like. When using a proteolytic enzyme, any of the above-described enzymes may be used alone or in combination. Further, as the amylolytic enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase and the like can be used. When using a amylolytic enzyme, any of the above-described enzymes may be used alone, or a plurality of them may be combined. Examples of the fibrinolytic enzyme include cellulase, hemicellulase, and pectinase. When a fibrinolytic enzyme is used, any of the above-described enzymes may be used alone, or a plurality may be combined. Examples of the lipolytic enzyme include lipase. In addition, when performing the hydrolysis process with an enzyme, you may use combining 1 or more types of 1 or more types of enzymes chosen from said each enzyme group.

  In the hydrolysis treatment using an enzyme, one or more of the above enzymes are added to the above-described plant extract solution, and the enzyme is reacted under conditions near the optimum pH and temperature of the enzyme used. To be implemented. When two or more kinds of enzymes are used in combination, two or more kinds of enzymes may be allowed to act simultaneously depending on the characteristics of the enzyme used, or may be allowed to act sequentially with or without changing reaction conditions. Good. The amount of the enzyme used is preferably in the range of 0.001 to 50 parts by weight, more preferably 0.1 to 10 parts by weight per one enzyme with respect to 100 parts by weight of the solid content of the plant extract solution. Range. The enzyme treatment time varies depending on the type of enzyme used and the like, but is generally in the range of 0.5 to 24 hours, preferably in the range of 1 to 6 hours.

  After completion of the hydrolysis treatment using the above enzyme, the enzyme is deactivated by using an appropriate method such as a method of heating the enzyme treatment liquid to, for example, 80 ° C. or higher to obtain an enzyme-treated decomposition product solution.

  The hydrolyzed product prepared as described above may generally be adjusted to a pH of 4 to 8 and used as it is as a cosmetic compounding agent, or at a desired concentration by vacuum concentration or the like. May be used as In addition, the extract may be dried by a conventional method such as spray drying.

  In addition, the hydrolyzate prepared as described above may be stored after refrigeration for a certain period of time in order to enhance storage stability and the like, and then the supernatant may be used.

  Examples of the preparation containing the hydrolyzate according to the present invention (including cosmetics and quasi-drugs) include emulsion, cream, lotion, essence, pack, lipstick, foundation, liquid foundation, makeup press powder, cheek red White powder, facial cleansers, body shampoos, hair shampoos, soaps, and the like, and hair growth agents, and further bath agents, but of course are not limited thereto.

  The amount of the extract of the present invention in the external preparation for skin is generally 0.002 to 1.0% by weight (the same applies hereinafter) in the case of basic cosmetics, preferably as the solid content of the hydrolyzate. Is in the range of 0.02 to 0.2% by weight, in the case of makeup cosmetics, generally in the range of 0.002 to 1.0% by weight, preferably in the range of 0.02 to 0.2% by weight. In the case of a material, it is generally in the range of 0.002 to 10.0% by weight, preferably 0.02 to 7.0% by weight. Further, in the case of hair cosmetics or hair restorers, the solid content of the extract is generally 0.0001 to 5.0% by weight, preferably 0.001 to 3.0% by weight. is there.

  In addition to the hydrolyzate, which is an essential component, components usually used for external preparations for skin and hair restorers, such as oily components, surfactants (synthetic and natural products), moisturizers, thickeners, antiseptics -A disinfectant, a powder component, an ultraviolet absorber, an antioxidant, a pigment, a fragrance and the like can be appropriately blended as necessary. Moreover, as long as the effectiveness and characteristics of the extract are not impaired, there may be no combination of other physiologically active ingredients.

  Here, as the oil component, for example, lotus oil, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice bran oil, rice germ oil, palm oil, chamomile oil, palm oil, cacao oil, meadow foam oil, rose Oils derived from plants such as hip oil, lambender oil, sheer butter, tea tree oil, avocado oil, macadamia nut oil, plant-derived squalane; oils derived from animals such as mink oil and turtle oil; beeswax, carnauba wax, rice wax , Waxes such as lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax, squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, eicosenoic acid; lauryl alcohol, cetanol, stearyl Higher alcohols such as alcohol; isopropyl myristate , Isopropyl palmitate, butyl oleate, 2-ethylhexyl glycerides, synthetic esters and synthetic triglycerides such as higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the like.

  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Anions such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates Surfactant; quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, Cationic surfactants such as N-dialkylmorphonium salts and polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene Amphoteric surfactants such as ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.

  Examples of emulsifiers or emulsifiers include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble Polysaccharides, soy-derived protein and polysaccharide complex, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponins and their Derivatives, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, legumes, millet, etc.) and the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, mucopolysaccharides (for example, hyaluron). Acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof, elastin and derivatives thereof, collagen and derivatives thereof, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, silane root (white and white) Examples include extracts, various amino acids, and derivatives thereof.

  Examples of thickeners include, for example, components derived from brown algae, green algae or red algae such as alginic acid, agar, carrageenan and fucoidan; silane root (white) extract; Gums such as tragacanth gum, locust bean gum and guar gum; cellulose derivatives such as carboxymethylcellulose and hydroxyethylcellulose; synthetic polymers such as polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid And its derivatives; polyglutamic acid and its derivatives.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Plants such as Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), Polyphosphoric acid, Propanediol, 1,2-Pentanediol, Various essential oils, Bark distillate, Radish fermentation liquor, Sugar cane Origin of ethanol or 1,3-butylene glycol.

  Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. System powder, cereal (rice, wheat, corn, millet, etc.) powder, legume (soybean, red bean, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, murasakixibub extract, silane root extract, peony extract, vitamin E and its derivatives (eg, vitamin E nicotinate, vitamin E linoleate, etc. ) Etc.

  Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone or derivatives thereof, ellagic acid and derivatives thereof, nicotinic acid and derivatives thereof, resorcinol derivatives, tranexamic acid and derivatives thereof, 4 -Methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended singly or in combination.

  Examples of the kojic acid derivative include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyltocopherylmaleic acid, ascorbyltocopherylphosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as prillyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L -L-ascorbic acid tetrafatty acid esters such as ascorbyl tetralaurate, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or acyl thereof Derivatives, ascorbyl glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl phosphate L-ascorbate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside), and tranexamic acid derivatives include tranexamic acid esters (for example, tranexam). Acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), amides of tranexamic acid (for example, tranexamic acid methylamide) and the like. Examples of resorcinol derivatives include 4-n-butylresorcinol, Examples of 2,5-dihydroxybenzoic acid derivatives such as 4-isoamylresorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxy. Cibenzoic acid and the like, nicotinic acid derivatives include, for example, nicotinic acid amide and benzyl nicotinate, and α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid, and the like. .

  Examples of the physiologically active component include placenta extract, Sakuhakuhi extract, Yukinoshita extract, perilla extract, rice bran extract or hydrolyzate thereof, white coconut extract or hydrolyzate thereof, fermented white coconut, peony Extract or hydrolyzate thereof, lactic acid bacteria fermented rice, murasakixikib extract, lotus seed extract or hydrolyzate thereof, lotus seed fermented product, pearl barley hydrolysate, barley seed fermented product, royal jelly fermented product, sake lees extract or it Examples include ceramides, fermented sake lees, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, chamomile extract and the like. Also, coral grass extract, rice leaf extract or hydrolyzate thereof, eggplant (lotus, long eggplant, Kamo eggplant, rice eggplant etc.) extract or hydrolyzate thereof, apricot fruit extract, catamen giraffe, etc. Seaweed extract of sea urchin, extract of marine flowering plant such as sea cucumber, fermented soymilk, jellyfish water, rice extract or hydrolyzate thereof, rice fermented extract, germinated rice extract or hydrolyzate thereof, germinated rice fermented Products, black bean extract or hydrolyzate thereof, damask rose flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid, etc.), animal or fish collagen and Derivatives thereof, elastin and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salts, etc.), t-cycloamino acid derivatives, vitamin A and derivatives thereof, allantoin, diisopropyl Pyramine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, carrot extract, ginseng extract or fermented product thereof, red ginseng extract, beetroot extract, loofah extract, anaasa extract , Peach extract, peach extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, paudarco bark extract, dairy lily extract or ferment, hibiscus flower extract or ferment, hagoromogusa Extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red rich extract, silane extract, yam peel or seed coat extract or hydrolysate, safflower flower extract, Casablanca Extract, sweet potato extract or fermented product thereof, guava leaf extract, dokudami extract, evening white Extract, aloe extract, fig flower extract, apple extract, there is a white asparagus extract or the like.

  Next, the present invention will be described more specifically with reference to production examples, formulation examples, and test examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all percentages are% by weight.

Production Example 1 Preparation of hydrolyzate (1)
1000 g of purified water was added to 100 g of dried shredded roots of Codonopsis pilosula and extracted at 4 ° C. for 12 hours. 700 u of α-amylase was added to 1000 g of the obtained extract, hydrolyzed at 55 ° C. for 1 hour, and then enzyme-inactivated at 80 ° C. for 1 hour. This liquid was filtered to obtain 720 g of a pale yellow transparent extract solution (solid content concentration: 1.7%).

Production Example 2 Preparation of hydrolyzate (2)
1000 g of purified water was added to 100 g of dried chopped carrot root and extracted at 80 ° C. for 1 hour. 800 g of papain was added to 1000 g of the obtained extract, hydrolyzed at 40 ° C. for 2 hours, and then enzyme-inactivated at 80 ° C. for 1 hour. The obtained extract was filtered to obtain 680 g of a yellow transparent extract solution (solid content concentration: 2.1%). Please add the enzymatic degradation process.

Production Example 3 Preparation of hydrolyzate (3)
1000 g of a 30% 1,3-butylene glycol aqueous solution was added to 100 g of dried shredded whole plant of lacquer carrot and extracted at 80 ° C. for 1 hour. 1500 g of α-amylase was added to 1000 g, hydrolyzed at 50 ° C. for 3 hours, and then enzyme-inactivated at 80 ° C. for 1 hour. The obtained extract was filtered to obtain 780 g of a yellowish brown transparent extract solution (solid content concentration: 2.1%).

Production Example 4 Preparation of hydrolyzate (4)
1000 g of a 50% ethanol aqueous solution was added to 100 g of dried chopped roots of sagenut carrot (Salicornea herbacea) and extracted at 4 ° C. for 12 hours. 500 AUN glucoamylase was added to 1000 g, hydrolyzed at 60 ° C. for 2 hours, and then enzyme-inactivated at 80 ° C. for 1 hour. The obtained extract was filtered to obtain 820 g of a pale yellow transparent extract solution (solid content concentration: 1.9%).

Production Example 5 Preparation of hydrolyzate (5)
In Production Example 1, 730 g of a yellow transparent extract solution (solid content concentration: 1.6%) was prepared in the same manner as in Production Example 1 except that roots of Codonopsis lanceolata were used instead of roots of lacquered carrot. Obtained.

Production Example 6 Preparation of hydrolyzate (6)
In Production Example 1, 680 g of a yellow transparent extract solution (solid content concentration: 2.2%) was prepared in the same manner as in Production Example 1 except that roots of Codonopsis tangshen were used in place of roots of lacquer carrot. Obtained.

Formulation Example 1 Lotion [Ingredients] part Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
Hydrolyzate of Production Example 1 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Potassium hydroxide appropriate amount Purified water Amount to make 100 parts in total Perfume Appropriate amount

Formulation Example 2 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 2 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 3 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 3 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 4 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1 except that 5.0 parts of the hydrolyzate in Production Example 4 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 5 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 5 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 6 Lotion Toner lotion was obtained in the same manner as in Formulation Example 1, except that 5.0 parts of the hydrolyzate in Production Example 6 was used instead of the hydrolyzate in Production Example 1 contained in Formulation Example 1.

Formulation Example 7 Latex [Ingredients] part Liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Lotus essential oil 0.025
Polyoxyethylene (20) sorbitan monostearate 1.0
Lipophilic glyceryl stearate 1.0
Hydrogenated soybean lecithin 1.5
Hydrolyzate of Production Example 1 3.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Water-soluble collagen 0.1
Purified water Total amount of 100 parts Perfume Suitable amount

Formulation Example 8 Emulsion An emulsion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of tranexamic acid was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 7. It was.

Formulation Example 9 Emulsion An emulsion was obtained in the same manner as in Formulation Example 7 except that 3.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide contained in Formulation Example 7. .

Formulation Example 10 Emulsion In the same manner as in Formulation Example 7, except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide contained in Formulation Example 7 are used, and the emulsion is used. Obtained.

Formulation Example 11 Emulsion [Ingredients] part squalane 3.0
Behenyl alcohol 3.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 1.0
Glycerin fatty acid ester 1.0
Soy lecithin 1.5
Hydrolyzate of Production Example 1 5.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Water-soluble collagen 0.1
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts

Formulation Example 12. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 1.0 part of tranexamic acid was used instead of 1.0 part of dipotassium glycyrrhizinate contained in Formulation Example 11.

Formulation Example 13 Lotion [component] part Hydrolyzate of Production Example 4 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Guar gum 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water Amount to make 100 parts in total

Formulation Example 14. Lotion A lotion was obtained in the same manner as in Formulation Example 13 except that 10.0 parts of the hydrolyzate in Production Example 5 was used instead of the hydrolyzate in Production Example 4 contained in Formulation Example 13.

Formulation Example 15. Essence [Ingredients] part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid 0.1
Hydrolyzed hyaluronic acid solution 0.1
Hydrolyzate of Production Example 6 5.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water totaling 100 parts

Example 16 Liquid foundation [ingredient] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Hydrolyzate of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount to be 100 parts Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount

Formulation Example 17. Body shampoo [ingredient] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Hydrolyzate of Production Example 1 5.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Formulation Example 18. Hair shampoo [ingredient] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Hydrolyzate of Production Example 2 2.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Example 19. Hair conditioner [Component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Hydrolyzate of Production Example 3 2.0
1,3-butylene glycol 5.0
Amount of purified water totaling 100 parts

Formulation Example 20. Hair restorer [ingredient] part dipotassium glycyrrhizinate 0.1
Mononitroguaiacol sodium 0.02
Pyridoxine hydrochloride 0.03
l-Menthol 0.8
Japanese cypress raft root extract 0.3
Brown algae extract 0.3
Panax ginseng extract 0.3
Assembly extract 2.0
Hydrolyzate of Production Example 1 3.5
Trimethylglycine 0.5
Lactic acid 0.2
1,3-butylene glycol 10.0
Phenoxyethanol 0.2
Polyoxyethylene hydrogenated castor oil 0.4
L-arginine appropriate amount ethanol 20.0
Amount of purified water totaling 100 parts

Test Example 1 MITF gene expression evaluation test
B16 cells were prepared to 6 × 10 ⁵ cells / mL in Eagle's minimum essential medium containing 10% FBS, 1 mL was seeded in a φ6 cm petri dish, and cultured at 37 ° C. in 5% CO ₂ and saturated steam. After culturing for 24 hours, each hydrolyzate was further added so that the final concentration as a solution was 10% with respect to the total amount of the culture solution containing the sample solution (hydrolyzate of Production Examples 1 to 6). And a theophylline-containing medium adjusted to a final concentration of 1 mM, and cultured under the same conditions for 4 days. In addition, as a comparative control, in place of the sample solution, a test group to which a culture solution containing only a PBS (−) solution (a final concentration of PBS (−) with respect to the total amount of the culture solution was adjusted to 10%) was added ( Control zone) was set. After culturing for 4 days, the cells in each test group were collected with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (manufactured by Wako Pure Chemical Industries, Ltd.) is added to the collected cells, stirred and mixed, and then centrifuged at 15,000 rpm, 4 ° C. for 15 minutes with a centrifuge (TOMY / MX-160) Only 400 μL of the aqueous layer was collected. To the recovered aqueous layer, 500 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, stirred and mixed, and centrifuged at 15,000 rpm at 4 ° C. for 15 minutes to obtain a total RNA precipitate. To this total RNA, 1 mL of 75% ethanol was added, stirred and washed, and centrifuged at 15,000 rpm, 4 ° C. for 15 minutes to collect a precipitate. The recovered total RNA was subjected to reverse transcription using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [manufactured by Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.), and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio Inc.] MITF gene expression and internal standard β-actin gene expression were detected. Here, β-actin is one of housekeeping genes (a gene that is expressed in a certain amount in common in many tissues and cells and is always expressed and essential for cell maintenance and proliferation). Since the expression level is always constant, it is used as an internal standard in PCR experiments. As a test result, the expression level of the MITF gene in each test section was compared when the expression level of the β-actin gene was constant. In this test system, the relative value of the expression level of each gene in other test plots when the expression level of each gene in the control plot was set to 100 was determined.

The results of Test Example 1 are shown in Table 1.
[Table 1]

  As shown in Table 1, the group to which the hydrolyzate according to the present invention was added showed an excellent MITF gene expression suppression effect. Here, MITF acts as a transcription factor and enhances the synthesis of melanin synthesis-related enzymes such as Tyrosinase, TRP1, and TRP2, and an excessively synthesized melanin synthesis-related enzyme eventually causes pigmentation. Therefore, it is suggested that the hydrolyzate according to the present invention has an excellent pigmentation-suppressing effect by suppressing the expression of the MITF gene.

Test Example 2 Evaluation test of blood flow promotion effect In Test Example 2, an evaluation test of blood flow promotion effect was performed using the backs of the left and right hands of the subjects (7 persons). First, wash the left and right hands, and test 2 cm x 2 cm against the left and right back of the subject who was waiting for 15 minutes in an air-conditioning control room (room temperature: 20 ± 2 ° C, humidity; 50 ± 5%). The site was set. Next, the measurer measured the initial surface temperature of each part from the front of the subject three times using a non-contact thermometer (Thermo Focus, manufactured by Tecnimed Srl) and recorded it as an initial value. Next, 700 mL of an aqueous solution containing 10% of the hydrolyzate of Production Examples 1 to 6 was prepared as a test solution, and 30% 1,3-butylene glycol solution was used instead of the hydrolyzate as a comparative control. A 700 mL aqueous solution was prepared. Each solution was put in a plastic container (1 L) and cooled to about 15 ° C. in advance. The subject immersed one hand in the test solution and the other hand in the control solution for 5 minutes to the wrist. After 5 minutes, the subject raises his hand and then wipes it quickly and thoroughly with a Kim towel, etc., and the measurer measures the surface temperature 3 times after 1, 3, 5, 7, 9, and 11 minutes after wiping. Measured and recorded. The amount of change (Δ ° C.) at each time was determined by subtracting the initial value from the average value of the surface temperature at each time.
The results of Test Example 2 are shown in Table 2. In this test example, 6 of 7 subjects showed effects, and the average value of 6 subjects who showed those effects is shown.

The results of Test Example 2 are shown in Table 2.
[Table 2]

  As shown in Table 2, it was clarified that the skin temperature was higher when the hydrolysis according to the present invention was added at any measurement time. Since the skin surface temperature is considered to represent the state of blood flow immediately below, the hydrolysis according to the present invention has the effect of promoting blood flow, prevents skin (scalp) aging, It is suggested that it has a sounding effect.

Claims (2)

  An external preparation for skin comprising a hydrolyzate of a plant of Campanulaceae or Codonopsis or an extract thereof as an active ingredient.   A hair restorer containing as an active ingredient a hydrolyzate of a plant of Campanulaceae or Codonopsis or an extract thereof.