JP2018143246A - Production method of lactic acid fermented bamboo sap - Google Patents

Production method of lactic acid fermented bamboo sap Download PDF

Info

Publication number
JP2018143246A
JP2018143246A JP2018078444A JP2018078444A JP2018143246A JP 2018143246 A JP2018143246 A JP 2018143246A JP 2018078444 A JP2018078444 A JP 2018078444A JP 2018078444 A JP2018078444 A JP 2018078444A JP 2018143246 A JP2018143246 A JP 2018143246A
Authority
JP
Japan
Prior art keywords
bamboo
lactic acid
powder
acid fermented
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2018078444A
Other languages
Japanese (ja)
Other versions
JP6499348B2 (en
Inventor
乃 玉井
Osamu Tamai
乃 玉井
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JP2018143246A publication Critical patent/JP2018143246A/en
Application granted granted Critical
Publication of JP6499348B2 publication Critical patent/JP6499348B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

PROBLEM TO BE SOLVED: To provide a lactic acid fermented bamboo sap which is not bulky, can be utilized easily according to an application, and is excellent in usefulness.SOLUTION: The lactic acid fermented bamboo sap 22 is obtained by combining a bamboo leaf powder 12, a bamboo root powder 13, and an acidic plant 37 having less than pH 7 into a lactic acid fermented bamboo powder 1 and by fermenting it in water and extracting it. The bamboo leaf powder 12 is 90 to 110% by weight of the lactic acid fermented bamboo powders 1, the bamboo root powder 13 is 30 to 50% by weight of the lactic acid fermented bamboo powder 1. The acidic plant 37 may be fruits of berries, citrus, and the like. For the fruits, berries, such as Cyanococcus and Rubus ursinus×idaeus, are suitable.SELECTED DRAWING: Figure 5

Description

本発明は、乳酸発酵竹液及びその製造方法に関するものである。   The present invention relates to a lactic acid fermented bamboo liquid and a method for producing the same.

孟宗竹を微細に切削又は粉砕した竹粉を乳酸発酵させた乳酸発酵竹粉がある。この乳酸発酵竹粉は、乳牛等の家畜用の飼料として良質のサイレージであると共に、農作物等の植物の生育を助ける良質の肥料として利用できる等、飼料用、肥料用、水産用、消臭用、食品用、化粧品用、医薬部外品用、医薬品用等の各種の分野での応用が広く期待されている。   There is a lactic acid fermented bamboo powder obtained by lactic acid fermentation of bamboo powder obtained by finely cutting or pulverizing Miso bamboo. This lactic acid fermented bamboo powder is good quality silage as feed for livestock such as dairy cattle and can be used as high quality fertilizer to help the growth of plants such as crops, for feed, fertilizer, fishery, deodorant etc. Applications in various fields such as foods, cosmetics, quasi-drugs, and pharmaceuticals are widely expected.

従来、この乳酸発酵竹粉は、孟宗竹の比較的若い生竹を500μm程度に切削して竹粉化し、この竹粉を短時間内に袋に詰めて封口し、その状態で2〜3週間静置して乳酸発酵させて製造している(特許文献1)。   Conventionally, this lactic acid fermented bamboo powder is cut into a bamboo powder by cutting a relatively young raw bamboo of Munetake bamboo to about 500 μm, and the bamboo powder is packed in a bag within a short time and sealed for 2 to 3 weeks. It is placed and fermented with lactic acid (Patent Document 1).

特開2006−180832号公報JP 2006-180832 A

従来の乳酸発酵竹粉は乳牛等の家畜用の飼料、農作物等の植物用の肥料、圃場用の土質改良材等として利用しているが、それ自体が粉体状であるため大きく嵩張ると共に、給餌、施肥等で他のものと混ぜ合わせる際の作業が非常に煩雑になる欠点がある。   Conventional lactic acid fermented bamboo powder is used as feed for livestock such as dairy cows, fertilizers for plants such as agricultural crops, soil quality improving materials for fields, etc. There is a drawback that the work when mixing with other things by feeding, fertilizing, etc. becomes very complicated.

また乳酸発酵竹粉は孟宗竹でも2〜3年程度の比較的若い生竹の竹粉を使用するとは云え、硬い竹繊維を多量に含んでいるため、乳牛等の家畜の飼料として給餌する場合には、その竹繊維で家畜が消化不良を招く等の惧れがある。しかし、乳酸発酵竹粉から硬い竹繊維のみを除去して給餌することは事実上不可能である。   In addition, lactic acid fermented bamboo powder is a relatively young raw bamboo powder that is used for 2 to 3 years. However, it contains a large amount of hard bamboo fiber, so it is used as feed for livestock such as dairy cows. The bamboo fiber may cause indigestion of livestock. However, it is practically impossible to remove and feed only hard bamboo fibers from lactic acid fermented bamboo powder.

更に従来の乳酸発酵竹粉は竹粉を一回の発酵工程で乳酸発酵させたに過ぎないため、乳酸と酢酸とを含むものの、多種類の有用な生菌を十分に生成できていない等、乳酸発酵竹粉が本来有する有用性をまだまだ十分に活用できていないという欠点がある。   Furthermore, since conventional lactic acid fermented bamboo powder is only lactic acid fermented with bamboo powder in a single fermentation process, it contains lactic acid and acetic acid, but has not been able to sufficiently produce many kinds of useful viable bacteria, There is a drawback that the usefulness inherent in lactic acid fermented bamboo powder has not been fully utilized.

本発明は、このような従来の問題点に鑑み、嵩張りがなく、用途に応じて容易に活用でき、しかも有用性に優れた乳酸発酵竹液及びその製造方法を提供することを目的とするものである。   In view of such conventional problems, the present invention aims to provide a lactic acid fermented bamboo liquid that is not bulky, can be easily used according to the application, and has excellent usability, and a method for producing the same. Is.

本発明の乳酸発酵竹液は、乳酸発酵竹粉に添加物を配合し水中で二次発酵させて二次発酵エキスを抽出したものである。   The lactic acid fermented bamboo liquid of the present invention is obtained by adding an additive to lactic acid fermented bamboo powder and subjecting it to secondary fermentation in water to extract the secondary fermented extract.

別の発明の乳酸発酵竹液は、乳酸発酵竹粉に竹葉粉と竹根粉とpH7未満の酸性質植物とを配合し水中で発酵させてエキスを抽出したものである。   The lactic acid fermented bamboo liquid of another invention is obtained by blending lactic acid fermented bamboo powder with bamboo leaf powder, bamboo root powder, and an acidic plant having a pH of less than 7, fermented in water and extracting the extract.

前記竹葉粉は前記乳酸発酵竹粉の90〜110重量%であり、前記竹根粉は前記乳酸発酵竹粉の30〜50重量%であることが望ましい。前記酸性質植物はベリー類、柑橘類等の果実でもよい。また前記果実はブルーベリー、ボイセンベリー等のベリー類であることが望ましい。前記ベリー類は前記乳酸発酵竹粉、前記竹葉粉及び前記竹根粉の総重量の3重量%以下であることが望ましい。   The bamboo leaf powder is preferably 90 to 110% by weight of the lactic acid fermented bamboo powder, and the bamboo root powder is preferably 30 to 50% by weight of the lactic acid fermented bamboo powder. The acidic plant may be a fruit such as berries and citrus fruits. The fruit is preferably a berry such as blueberry or boysenberry. The berries are preferably 3% by weight or less of the total weight of the lactic acid fermented bamboo powder, the bamboo leaf powder and the bamboo root powder.

本発明の乳酸発酵竹液の製造方法は、竹粉を一次乳酸発酵させた乳酸発酵竹粉に竹葉粉と竹根粉とpH7未満の酸性質植物とを配合し、この配合後の乳酸発酵竹粉を水中に浸漬して二次発酵させてエキスを抽出するものである。   The method for producing a lactic acid-fermented bamboo liquid according to the present invention comprises blending bamboo leaf powder, bamboo root powder, and an acidic plant having a pH of less than 7 into lactic acid-fermented bamboo powder obtained by subjecting bamboo powder to primary lactic acid fermentation. The bamboo powder is immersed in water and subjected to secondary fermentation to extract the extract.

その場合、一次乳酸発酵を真空下で行うことが望ましい。また前記竹葉粉、前記竹根粉及び酸性質植物を配合した乳酸発酵竹粉を浸漬容器内の水中に浸漬した後、該浸漬容器内の空気を不活性ガスに置換して二次発酵を行うこともある。エキスの抽出後にオートクレーブ滅菌を行うこともある。   In that case, it is desirable to perform primary lactic acid fermentation under vacuum. Moreover, after immersing the lactic acid fermented bamboo powder containing the bamboo leaf powder, the bamboo root powder and the acidity plant in water in an immersion container, the air in the immersion container is replaced with an inert gas to perform secondary fermentation. Sometimes. Autoclave sterilization may be performed after extraction of the extract.

本発明によれば、嵩張りがなく、用途に応じて容易に活用でき、しかも有用性に優れた乳酸発酵竹液を製造できる利点がある。   According to the present invention, there is an advantage that a lactic acid fermented bamboo liquid which is not bulky, can be easily used according to the application, and has excellent usability can be produced.

本発明の第1の実施形態を示す乳酸発酵竹粉の製造方法の説明図である。It is explanatory drawing of the manufacturing method of the lactic acid fermentation bamboo powder which shows the 1st Embodiment of this invention. 本発明の第2の実施形態を示す乳酸発酵竹粉の製造方法の説明図である。It is explanatory drawing of the manufacturing method of the lactic acid fermentation bamboo powder which shows the 2nd Embodiment of this invention. 本発明の第3の実施形態を示す乳酸発酵竹液の製造方法の説明図である。It is explanatory drawing of the manufacturing method of the lactic acid fermented bamboo liquid which shows the 3rd Embodiment of this invention. 同酢酸エチルの生成の有無を確認するデータを示す図である。It is a figure which shows the data which confirms the presence or absence of the production | generation of the same ethyl acetate. 本発明の第4の実施形態を示す乳酸発酵竹液の製造方法の説明図である。It is explanatory drawing of the manufacturing method of the lactic acid fermented bamboo liquid which shows the 4th Embodiment of this invention. 同有機酸の生成のデータを示す図である。It is a figure which shows the production | generation data of the same organic acid. 同アミノ酸の生成のデータを示す図である。It is a figure which shows the production | generation data of the same amino acid. 本発明の第5の実施形態を示す乳酸発酵竹液の製造方法の説明図である。It is explanatory drawing of the manufacturing method of the lactic acid fermented bamboo liquid which shows the 5th Embodiment of this invention. 同有機酸の生成のデータを示す図である。It is a figure which shows the production | generation data of the same organic acid. 同アミノ酸の生成のデータを示す図である。It is a figure which shows the production | generation data of the same amino acid.

以下、本発明の実施形態を図面に基づいて詳述する。   Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

図1は酢酸エチルの生成を防止しながら飼料用(畜産用)の乳酸発酵竹粉1を製造する本発明の第1の実施形態を示す。乳酸発酵竹粉1は図1に示す竹粉化工程2、金属除去工程3、真空乳酸発酵工程4、篩工程5、配合工程6、真空包装工程8を経て製造する。   FIG. 1 shows a first embodiment of the present invention for producing lactic acid fermented bamboo powder 1 for feed (for livestock) while preventing the production of ethyl acetate. The lactic acid fermented bamboo powder 1 is manufactured through the bamboo powdering process 2, the metal removing process 3, the vacuum lactic acid fermentation process 4, the sieving process 5, the blending process 6, and the vacuum packaging process 8 shown in FIG.

竹粉化工程2では2〜3年物の孟宗竹の生竹9を使用し、この生竹9を切削して所定の大きさに竹粉化する。この竹粉化に際しては、例えば生竹9の端面に当接して回転する回転刃10を備えた竹粉化装置を利用し、その回転刃10により生竹9を端面側から長手方向と略直角方向に切削して所定の大きさの竹粉11にする。なお、竹粉11は生竹9を切削したものであって、所定の水分を含有している。   In the bamboo powdering step 2, raw bamboo 9 of 2 years old is used, and this raw bamboo 9 is cut into a predetermined size. For this bamboo powdering, for example, a bamboo powdering device including a rotary blade 10 that rotates in contact with the end face of the raw bamboo 9 is used. The rotary blade 10 causes the raw bamboo 9 to be substantially perpendicular to the longitudinal direction from the end face side. It cuts in the direction to make the bamboo powder 11 of a predetermined size. Bamboo powder 11 is obtained by cutting raw bamboo 9 and contains predetermined moisture.

竹粉11は略500μm程度の厚さで生竹9を横方向に切削してハニカム状にしたものが適当であるが、約50〜600μm程度でもよい。また竹粉11は生竹9を他の方法で粉砕したものでもよい。竹粉化後の竹粉11は、空気搬送等により次の金属除去工程3に搬送する。   The bamboo powder 11 having a thickness of about 500 μm and a raw bamboo 9 cut in the lateral direction to form a honeycomb is suitable, but it may be about 50 to 600 μm. Bamboo powder 11 may be obtained by pulverizing raw bamboo 9 by other methods. The bamboo powder 11 after being made into bamboo powder is conveyed to the next metal removal step 3 by air conveyance or the like.

金属除去工程3では竹粉11を金属除去装置にかけて、竹粉化工程2での回転刃10の刃零れ等により竹粉11中に混入した金属片を磁石により吸着して除去する。これは家畜類に給餌したときに、体内に金属片が入らないようにするためである。この金属除去処理後の竹粉11は、空気搬送等により次の真空乳酸発酵工程4へと搬送する。   In the metal removal step 3, the bamboo powder 11 is applied to a metal removal device, and the metal pieces mixed in the bamboo powder 11 are removed by suction with a magnet by the blade spilling of the rotary blade 10 in the bamboo powdering step 2. This is to prevent metal pieces from entering the body when feeding livestock. The bamboo powder 11 after the metal removal treatment is conveyed to the next vacuum lactic acid fermentation step 4 by air conveyance or the like.

なお、竹粉化工程2と金属除去工程3との間に粗篩工程を設けて、竹粉11を粗い篩にかけて、竹粉化工程2で切削されなかった大きな(例えば数百mm以上の大きさ)未切削片を除去してもよい。この場合には、金属除去工程3での金属除去性能を向上させることができる。粗篩工程は金属除去工程3と真空乳酸発酵工程4との間に設けてもよい。これらに関しても空気搬送を利用してもよい。   In addition, a rough sieving process is provided between the bamboo powdering process 2 and the metal removing process 3, and the bamboo powder 11 is passed through a coarse sieve so that it is not cut in the bamboo powdering process 2 (for example, a size of several hundred mm or more). A) Uncut pieces may be removed. In this case, the metal removal performance in the metal removal step 3 can be improved. The coarse sieving step may be provided between the metal removal step 3 and the vacuum lactic acid fermentation step 4. In these cases, air conveyance may be used.

真空乳酸発酵工程4では真空装置40の真空容器(真空処理室)41内に、適度な水分を含有する竹粉11を入れて所定の真空下で所定期間静置することにより、竹由来の乳酸菌で竹粉11を乳酸発酵(一次乳酸発酵)させて一次乳酸発酵竹粉(以下、乳酸発酵原料竹粉という)14を生成する。   In the vacuum lactic acid fermentation process 4, bamboo powder 11 containing appropriate moisture is placed in a vacuum container (vacuum processing chamber) 41 of a vacuum apparatus 40 and left standing for a predetermined period under a predetermined vacuum, whereby lactic acid bacteria derived from bamboo Then, the bamboo powder 11 is subjected to lactic acid fermentation (primary lactic acid fermentation) to produce a primary lactic acid fermentation bamboo powder (hereinafter referred to as lactic acid fermentation raw material bamboo powder) 14.

真空装置40は、所定の真空圧に十分に耐え得る密封度の高い箱状等の真空容器41と、この真空容器41内の気体を吸引して排出する真空系統42と、真空容器41内に竹粉11を搬入する搬入系統43と、真空容器41内の乳酸発酵原料竹粉14を搬出する搬出系統44とを備えている。   The vacuum device 40 has a highly sealed box-like vacuum container 41 that can sufficiently withstand a predetermined vacuum pressure, a vacuum system 42 that sucks and discharges the gas in the vacuum container 41, and a vacuum container 41. A carry-in system 43 for carrying in the bamboo powder 11 and a carry-out system 44 for carrying out the lactic acid fermentation raw material bamboo powder 14 in the vacuum vessel 41 are provided.

真空容器41はステンレス板等の金属板により箱状、その他の形状に構成されており、側壁、天井等の所要箇所に点検窓45と、この点検窓45を開閉する開閉蓋46とが設けられている。点検窓45は真空容器41の内部の点検用であり、開閉蓋46は点検窓45を経て真空容器41の内部を視認できるようになっている。なお、真空容器41内の真空度が高い場合には、真空容器41は球形状にしてもよい。また真空容器41の容積が大きい場合には、点検窓45を通じて作業員が出入りできるようにしてもよい。   The vacuum vessel 41 is configured in a box shape or other shape by a metal plate such as a stainless steel plate, and an inspection window 45 and an opening / closing lid 46 for opening and closing the inspection window 45 are provided at required portions such as a side wall and a ceiling. ing. The inspection window 45 is for inspecting the inside of the vacuum vessel 41, and the opening / closing lid 46 can visually recognize the inside of the vacuum vessel 41 through the inspection window 45. In addition, when the degree of vacuum in the vacuum vessel 41 is high, the vacuum vessel 41 may be spherical. When the volume of the vacuum vessel 41 is large, an operator may be able to enter and exit through the inspection window 45.

真空系統42は真空容器41の上部等に接続された吸引用配管47を有し、この吸引用配管47にはゴミを除去する二次フィルタ48と、真空容器41内の圧力を測定する圧力計49と、電磁式、電動式、手動式等の開閉弁50と、真空容器41内の空気等を吸引する真空ポンプ51とが設けられ、また真空系統42の真空容器41内には竹粉11等を除去する一次フィルタ52が設けられている。   The vacuum system 42 has a suction pipe 47 connected to the upper part of the vacuum vessel 41 and the like. The suction pipe 47 has a secondary filter 48 for removing dust and a pressure gauge for measuring the pressure in the vacuum vessel 41. 49, an on-off valve 50 such as an electromagnetic type, an electric type, or a manual type, and a vacuum pump 51 for sucking air or the like in the vacuum vessel 41 are provided. In the vacuum vessel 41 of the vacuum system 42, bamboo powder 11 is provided. A primary filter 52 is provided to remove and the like.

搬入系統43は真空容器41の側壁の上部又は天井に形成された入口部54と、入口部54に接続された空気搬送用の搬入用配管55と、搬入用配管55の途中に設けられた電磁式、電動式、手動式等の開閉弁56とを有し、竹粉11を空気搬送により搬入するようになっている。   The carry-in system 43 includes an inlet 54 formed on the top or ceiling of the side wall of the vacuum vessel 41, an air carrying-in pipe 55 connected to the inlet 54, and an electromagnetic provided in the middle of the carry-in pipe 55. It has an on-off valve 56 such as an electric type, an electric type, and a manual type, and the bamboo powder 11 is carried in by air conveyance.

搬出系統44は真空容器41の側壁の下部又は最下部に形成された出口部57と、出口部57に接続された空気搬送用の搬出用配管58と、搬出用配管58の途中に設けられた電磁式、電動式、手動式等の開閉弁59とを有し、真空容器41内の乳酸発酵後の乳酸発酵原料竹粉14を空気搬送により搬出するようになっている。   The unloading system 44 is provided in the middle of the outlet 57 formed at the lower or lowermost side of the side wall of the vacuum vessel 41, the unloading piping 58 connected to the outlet 57, and the unloading piping 58. An open / close valve 59 such as an electromagnetic type, an electric type, and a manual type is provided, and the lactic acid fermentation raw material bamboo powder 14 after lactic acid fermentation in the vacuum vessel 41 is carried out by air conveyance.

なお、真空容器41は、その内部の竹粉11、乳酸発酵原料竹粉14等のpHを検出するpH検出器(図示省略)を備えている。   The vacuum vessel 41 includes a pH detector (not shown) for detecting the pH of the bamboo powder 11 and the lactic acid fermentation raw material bamboo powder 14 inside.

真空装置40では搬入系統43の開閉弁56を開状態にして、搬入用配管55を経て空気搬送により真空容器41内へと竹粉11を搬入する。このとき搬出用配管58の開閉弁59を開状態にするか、又は吸引用配管47の開閉弁50を開状態にして、真空容器41内の空気を容易に排出できるようにする。   In the vacuum device 40, the open / close valve 56 of the carry-in system 43 is opened, and the bamboo powder 11 is carried into the vacuum container 41 by air conveyance through the carry-in piping 55. At this time, the open / close valve 59 of the carry-out pipe 58 is opened or the open / close valve 50 of the suction pipe 47 is opened so that the air in the vacuum vessel 41 can be easily discharged.

真空容器41への竹粉11の空気搬入が終了すると、搬入用配管55の開閉弁56及び搬出用配管58の開閉弁59を閉状態とし、吸引用配管47の開閉弁50を開状態にし、真空ポンプ51により真空容器41内の空気を吸引して減圧する。そして、例えば圧力計49の計測値が絶対圧力50KPa程度、望ましくは0.1Pa程度まで下れば開閉弁50を閉状態にし、真空容器41内を真空(低真空)状態に維持する。この真空下では真空容器41内が低酸素雰囲気下となっている。なお、真空容器41内の圧力は100Pa以上、例えば50KPa程度の低真空でもよいし、また0.1〜100Pa程度の中真空としてもよい。   When the air loading of the bamboo powder 11 into the vacuum vessel 41 is completed, the opening / closing valve 56 of the loading pipe 55 and the opening / closing valve 59 of the unloading pipe 58 are closed, and the opening / closing valve 50 of the suction pipe 47 is opened, The vacuum pump 51 sucks the air in the vacuum container 41 to reduce the pressure. For example, when the measured value of the pressure gauge 49 is reduced to an absolute pressure of about 50 KPa, preferably about 0.1 Pa, the on-off valve 50 is closed and the inside of the vacuum vessel 41 is maintained in a vacuum (low vacuum) state. Under this vacuum, the inside of the vacuum vessel 41 is in a low oxygen atmosphere. The pressure in the vacuum container 41 may be 100 Pa or more, for example, a low vacuum of about 50 KPa, or a medium vacuum of about 0.1 to 100 Pa.

真空容器41内の温度は常温(例えば25°C前後)としている。また真空容器41内のpH検出器(図示省略)で乳酸発酵原料竹粉14のpHが検出可能となっている。真空乳酸発酵工程4での一次乳酸発酵中は、pH検出器により真空容器41内のpH値を計測しており、そのpH値が4になったときに乳酸発酵が十分に進んだと判断でき、真空乳酸発酵工程4を終了する。この一次乳酸発酵に要する期間は例えば5日程度であり、この5日間の乳酸発酵によって乳酸発酵原料竹粉14ができる。   The temperature in the vacuum vessel 41 is set to room temperature (for example, around 25 ° C.). Further, the pH of the lactic acid fermentation raw material bamboo powder 14 can be detected by a pH detector (not shown) in the vacuum container 41. During the primary lactic acid fermentation in the vacuum lactic acid fermentation process 4, the pH value in the vacuum container 41 is measured by the pH detector, and when the pH value becomes 4, it can be determined that the lactic acid fermentation has sufficiently progressed. Then, the vacuum lactic acid fermentation step 4 is completed. The period required for the primary lactic acid fermentation is, for example, about 5 days, and the lactic acid fermentation raw material bamboo powder 14 is formed by the lactic acid fermentation for 5 days.

また乳酸発酵期間中、真空容器41内の乳酸発酵原料竹粉14のpH値を管理することにより、pH異常発酵(つまり、所定静置期間が経過しているにも拘わらずpHが下がらないという異常発酵)を検出できる利点もある。   Further, during the lactic acid fermentation period, by managing the pH value of the lactic acid fermentation raw material bamboo powder 14 in the vacuum vessel 41, it is said that the pH does not drop despite the fact that the predetermined stationary period has elapsed. There is also an advantage that abnormal fermentation) can be detected.

即ち、通常はpH値が4に低下して正常発酵が完了する期間(正常完了期間:前述の5日間)で正常発酵するが、5日間を経過してもpH値が下がらない(或いは上がっている場合もある)異常発酵が生じる場合がある。しかし、真空容器41内の乳酸発酵原料竹粉14のpH値を計測し管理することにより、異常発酵を的確に検出することができ、異常発酵品の製品化を排除できる。   That is, normal fermentation usually takes place during the period when the pH value drops to 4 and normal fermentation is completed (normal completion period: the aforementioned 5 days), but the pH value does not drop (or rises) even after 5 days. In some cases, abnormal fermentation may occur. However, by measuring and managing the pH value of the lactic acid fermentation raw material bamboo powder 14 in the vacuum vessel 41, abnormal fermentation can be accurately detected, and commercialization of abnormal fermentation products can be eliminated.

なお、pH検出器で検出されたpH値を例えばコンピュータ等の制御手段(図示省略)に出力し、そのpH値が予め設定された設定値(例えば「4」)に到達したときに真空乳酸発酵工程4を終了するようにしてもよい。また正常完了期間内にpH値が設定値に到達すれば正常発酵とし、到達していなければ異常発酵と判定することができる。   The pH value detected by the pH detector is output to a control means (not shown) such as a computer, and when the pH value reaches a preset value (for example, “4”), vacuum lactic acid fermentation You may make it complete | finish the process 4. FIG. Moreover, it can be determined as normal fermentation if the pH value reaches the set value within the normal completion period, and it can be determined as abnormal fermentation if not reached.

またコンピュータ等の制御手段に、正常発酵の場合のpH遷移を示す正常遷移パターンデータを予め記憶しておき、計測したpH値のパターンデータが正常遷移パターンデータと一致または許容範囲内にあることに基づいて、真空乳酸発酵工程4の終了と判定するようにしてもよい。   Further, normal transition pattern data indicating a pH transition in the case of normal fermentation is stored in advance in a control means such as a computer, and the measured pH value pattern data is coincident with the normal transition pattern data or within an allowable range. Based on this, the end of the vacuum lactic acid fermentation step 4 may be determined.

真空容器41が大型の場合や複数の区画室を備える場合には、真空容器41の内部のpH値を平均的に満遍なく検出できるように複数のpH検出器を配設することにより、各計測部位毎に乳酸発酵の良否を判定することができる。   When the vacuum container 41 is large or has a plurality of compartments, by arranging a plurality of pH detectors so that the pH value inside the vacuum container 41 can be detected evenly on average, The quality of lactic acid fermentation can be determined every time.

真空容器41はステンレス板等の金属板を真空用材料として使用しており、真空容器41内にヘテロ乳酸菌の発酵による炭酸ガスが発生したときにも、ガス漏れや変形が生じない構成となっている。なお、真空容器41はステンレス板等の金属板を使用したものである必要はなく、耐真空性、密封性を有するものであれば、合成樹脂製の可撓シート材を利用した袋等でもよい。   The vacuum vessel 41 uses a metal plate such as a stainless steel plate as a vacuum material, and does not leak or deform even when carbon dioxide is generated in the vacuum vessel 41 due to fermentation of heterolactic acid bacteria. Yes. The vacuum container 41 does not need to use a metal plate such as a stainless steel plate, and may be a bag using a flexible sheet material made of synthetic resin as long as it has vacuum resistance and sealing performance. .

真空乳酸発酵工程4での乳酸発酵が終了すると、開閉弁50を開状態にし、真空容器41内を大気圧状態に戻した後、搬出用配管58の開閉弁59を開状態として、空気搬送により、真空容器41内の乳酸発酵原料竹粉14を搬出用配管58を通じて次の篩工程5へと搬送する。   When the lactic acid fermentation in the vacuum lactic acid fermentation step 4 is completed, the on-off valve 50 is opened, the inside of the vacuum vessel 41 is returned to the atmospheric pressure state, the on-off valve 59 of the unloading pipe 58 is opened, and air conveyance is performed. Then, the lactic acid fermentation raw material bamboo powder 14 in the vacuum container 41 is conveyed to the next sieving step 5 through the unloading pipe 58.

篩工程5では乳酸発酵原料竹粉14を篩にかけて500μm以下、望ましくは300μm程度以下に揃える。この時点で篩にかけることにより、乳酸発酵原料竹粉14自体の水分の含有量が竹粉11に比較して少ないので、篩の目詰まりが少なくなり能率よく効率的に篩い分けることができる。   In the sieving step 5, the lactic acid fermentation raw material bamboo powder 14 is sieved to 500 μm or less, preferably about 300 μm or less. By sieving at this point, the water content of the lactic acid fermentation raw material bamboo powder 14 itself is less than that of the bamboo powder 11, so that clogging of the sieve is reduced and the sieving can be efficiently and efficiently screened.

配合工程6では篩にかけた後の乳酸発酵原料竹粉14を攪拌機に入れ、この乳酸発酵原料竹粉14に添加物である植物酵素15と竹炭粉16とを添加して配合する。植物酵素15はその分解、合成、吸収作用により家畜に不可欠な新陳代謝を促進させて活性化を図るためであり、この植物酵素15としては例えばパパイン酵素が適当である。竹炭粉16は乳酸発酵竹粉1の臭気を脱臭し、そのpHの中性化を図るためである。   In the blending step 6, the lactic acid fermentation raw material bamboo powder 14 after sieving is put into a stirrer, and the plant enzyme 15 and the bamboo charcoal powder 16 as additives are added to the lactic acid fermentation raw material bamboo powder 14 and blended. The plant enzyme 15 is intended to promote activation by promoting metabolism necessary for livestock by its decomposition, synthesis, and absorption, and for example, papain enzyme is suitable as the plant enzyme 15. The bamboo charcoal powder 16 is for deodorizing the odor of the lactic acid fermented bamboo powder 1 and neutralizing its pH.

飼料用の乳酸発酵竹粉1の場合には対象とする家畜の種類によっても異なるが、例えば乳牛用であれば乳酸発酵原料竹粉14を10kgに対して植物酵素15を60g、竹炭粉16を200g程度配合するのが適当である。   In the case of lactic acid fermented bamboo powder 1 for feed, it varies depending on the type of livestock to be used. For dairy cattle, for example, 10 kg of lactic acid fermented raw material bamboo powder 14 per 60 kg of plant enzyme 15 and bamboo charcoal powder 16 It is appropriate to blend about 200 g.

乳酸発酵後の乳酸発酵原料竹粉14は強い酸性を示し、また特有の強い臭気を有する。しかし、アルカリ性の竹炭粉16を配合することにより、その中和作用によりpHを乳酸発酵終了時点よりも中性に近付けることができ、また特有の臭気を脱臭することができる。なお、竹炭粉16を多量に配合すると、乳酸発酵竹粉1の色合いが黒くなり、乳牛等の食欲を損なうことになる。   The lactic acid fermentation raw material bamboo powder 14 after lactic acid fermentation shows strong acidity and has a unique strong odor. However, by blending the alkaline bamboo charcoal powder 16, the neutralization action makes it possible to bring the pH closer to neutrality than the end of lactic acid fermentation, and it is possible to deodorize a specific odor. In addition, when bamboo charcoal powder 16 is mix | blended in large quantities, the color of lactic acid fermentation bamboo powder 1 will become black, and appetite of dairy cows etc. will be impaired.

このように乳酸発酵原料竹粉14に植物酵素15及び竹炭粉16の添加物を配合すれば、真空包装工程8において、その添加物が配合された乳酸発酵竹粉1を所定量ずつ計量して密封容器等の密封袋17により真空包装して最終の製品18とする。密封袋17には遮光性、密封性を有するアルミ蒸着フィルム製のものを使用する。   Thus, if the additive of the plant enzyme 15 and the bamboo charcoal powder 16 is mix | blended with the lactic-acid-fermentation raw material bamboo powder 14, in the vacuum packaging process 8, the lactic-acid-fermented bamboo powder 1 with which the additive was mix | blended will be measured for every predetermined amount. A final product 18 is obtained by vacuum packaging with a sealed bag 17 such as a sealed container. The sealing bag 17 is made of an aluminum vapor-deposited film having light shielding properties and sealing properties.

このようにして製造した乳酸発酵竹粉1は、本来家畜類の成長促進効果の大きい良質のサイレージの一種であるが、植物酵素15を配合することによって、難点であった竹繊維の問題を解消することができる。   The lactic acid fermented bamboo powder 1 produced in this way is a kind of high-quality silage that originally has a large effect of promoting the growth of livestock, but by incorporating the plant enzyme 15, the problem of bamboo fiber, which was a difficult point, is solved. can do.

即ち、乳酸発酵竹粉1に植物酵素15、取り分けパパイン酵素を配合しているため、家畜の体内でのパパイン酵素の働きにより乳酸発酵竹粉1の竹繊維を容易に軟化又は分解でき、竹繊維が原因の消化不良等を防止できると共に、家畜類の新陳代謝を促進でき、乳酸発酵竹粉1が本来的に有する成長促進効果を更に増大させることができる。   That is, since the lactic acid fermented bamboo powder 1 contains the plant enzyme 15 and, in particular, the papain enzyme, the bamboo fiber of the lactic acid fermented bamboo powder 1 can be easily softened or decomposed by the action of the papain enzyme in the body of the livestock. Can prevent indigestion caused by, and can promote the metabolism of livestock, and can further increase the growth promoting effect inherent in the lactic acid fermented bamboo powder 1.

また乳酸発酵竹粉1には竹炭粉16を配合しているため、特有の臭気、酸性を嫌う家畜類の飼料用としても最適であり、各種の家畜類に広く給餌することが可能である。   Moreover, since the lactic acid fermented bamboo powder 1 is blended with bamboo charcoal powder 16, it is optimal for feed for livestock that dislikes a specific odor and acidity, and can be widely fed to various livestock.

植物酵素15、竹炭粉16の配合割合は、家畜類の嗜好性を考慮して適宜調節可能である。例えば植物酵素15としてのパパイン酵素の配合割合は、乳酸発酵原料竹粉14の10Kgに対して40〜80g(0.004〜0.008重量%)の範囲で調節可能であり、また竹炭粉16の配合割合は、乳酸発酵原料竹粉14の10Kgに対して100〜300g(0.01〜0.03重量%)の範囲で調節可能である。   The mixing ratio of the plant enzyme 15 and the bamboo charcoal powder 16 can be appropriately adjusted in consideration of the palatability of livestock. For example, the blending ratio of the papain enzyme as the plant enzyme 15 can be adjusted within a range of 40 to 80 g (0.004 to 0.008% by weight) with respect to 10 kg of the lactic acid fermentation raw material bamboo powder 14, and the bamboo charcoal powder 16 The blending ratio of can be adjusted in the range of 100 to 300 g (0.01 to 0.03% by weight) with respect to 10 kg of lactic acid fermentation raw material bamboo powder 14.

更に真空乳酸発酵工程4では、耐真空性を有する真空容器41を使用して真空下(低酸素雰囲気下)で竹粉11を乳酸発酵させているため、ヘテロ乳酸菌による乳酸発酵時に炭酸ガスが発生しても、それによる内圧の上昇によって真空容器41が損傷するようなことがない。従って、真空乳酸発酵工程4での乳酸発酵中に外部の雑菌類が進入して内部で増殖する等による、乳酸発酵竹粉1の品質の低下を防止できる。   Furthermore, in the vacuum lactic acid fermentation process 4, since the bamboo powder 11 is lactic acid fermented under vacuum (under a low oxygen atmosphere) using a vacuum vessel 41 having vacuum resistance, carbon dioxide gas is generated during lactic acid fermentation by heterolactic bacteria. Even so, the vacuum vessel 41 is not damaged by the increase in internal pressure. Therefore, the deterioration of the quality of the lactic acid fermented bamboo powder 1 due to the entry of external germs during the lactic acid fermentation in the vacuum lactic acid fermentation step 4 and the proliferation inside can be prevented.

また仮に真空乳酸発酵工程4以前の段階で竹粉11等に、好気性菌であるハンゼヌラ又はピチア等の酵母が混入するようなことがあっても、真空容器41内を真空状態に維持して、その真空下で竹粉11を乳酸発酵させているため、酵母菌の繁殖を抑制することができ、竹粉11の乳酸発酵中に酢酸とエタノールとのエステル反応により生成される酢酸エチルの発生を未然に防止することができる。このため乳酸発酵竹粉1の安全性が著しく向上する利点がある。   In addition, even if yeast such as Hansenula or Pichia, which are aerobic bacteria, may be mixed in the bamboo powder 11 or the like before the vacuum lactic acid fermentation step 4, the inside of the vacuum container 41 is maintained in a vacuum state. Since the bamboo powder 11 is lactic acid fermented under the vacuum, the growth of yeast can be suppressed, and the generation of ethyl acetate generated by the ester reaction of acetic acid and ethanol during the lactic acid fermentation of the bamboo powder 11 Can be prevented in advance. For this reason, there exists an advantage which the safety | security of the lactic acid fermentation bamboo powder 1 improves remarkably.

なお、真空容器41を含む真空装置40を複数設けて、各真空容器41に順次日にちを変えて竹粉11を入れて真空発酵を開始する等、各真空容器41毎に乳酸発酵の開始時期を1日単位で順次ずらすような製造管理方式を採用すれば、乳酸発酵竹粉1の製造に数日の日数を要するにも拘わらず、その生産性の向上を図ることができる。例えば、真空容器41を含む真空装置40を5台設置すれば、その5台を月曜日から金曜日までの5日間に順次割り当てて製造することも可能である。勿論、その設置数は5台以外の複数であってもよい。   It should be noted that a plurality of vacuum devices 40 including the vacuum containers 41 are provided, and the start date of lactic acid fermentation is set for each vacuum container 41, such as changing the date sequentially into each vacuum container 41 and putting the bamboo powder 11 into the vacuum fermentation. By adopting a production management system that sequentially shifts in units of one day, it is possible to improve the productivity even though it takes several days to produce the lactic acid fermented bamboo powder 1. For example, if five vacuum devices 40 including the vacuum vessel 41 are installed, it is also possible to manufacture them by sequentially allocating the five to five days from Monday to Friday. Of course, the number of installations may be plural other than five.

図2は肥料用の乳酸発酵竹粉1を製造する本発明の第2の実施形態を示す。   FIG. 2 shows a second embodiment of the present invention for producing a lactic acid fermented bamboo powder 1 for fertilizer.

肥料用の乳酸発酵竹粉1は、図2に示す竹粉化工程2、金属除去工程3、真空乳酸発酵工程4、篩工程5、配合工程6、二次発酵工程7、包装工程8Aを経て製造する。   The lactic acid fermented bamboo powder 1 for fertilizer passes through the bamboo powdering process 2, the metal removal process 3, the vacuum lactic acid fermentation process 4, the sieving process 5, the compounding process 6, the secondary fermentation process 7 and the packaging process 8A shown in FIG. To manufacture.

なお、竹粉化工程2、金属除去工程3、真空乳酸発酵工程4、篩工程5までは、第1の実施形態と略同じである。肥料用の場合には、金属除去工程3は省略してもよい。   Note that the bamboo powdering step 2, the metal removing step 3, the vacuum lactic acid fermentation step 4, and the sieving step 5 are substantially the same as those in the first embodiment. In the case of fertilizer, the metal removal step 3 may be omitted.

配合工程6では、真空乳酸発酵工程4で一次乳酸発酵させた後の乳酸発酵原料竹粉14に、パパイン酵素等の植物酵素15と竹炭粉16と竹葉粉20との添加物を添加し配合する。この場合、乳酸発酵原料竹粉14を10kgに対してパパイン酵素等の植物酵素15を20g(乳酸発酵原料竹粉14に対して0.002重量%)、竹炭粉16を800g(乳酸発酵原料竹粉14に対して0.08重量%)、竹葉粉20を10kg(乳酸発酵原料竹粉14と略同じ)程度の割合で配合する。   In the blending process 6, additives such as papain enzyme and other plant enzymes 15, bamboo charcoal powder 16, and bamboo leaf powder 20 are added to the lactic acid fermentation raw material bamboo powder 14 after the primary lactic acid fermentation in the vacuum lactic acid fermentation process 4. To do. In this case, 10 g of lactic acid fermentation raw material bamboo powder 14 is 20 g of plant enzyme 15 such as papain enzyme (0.002% by weight with respect to lactic acid fermentation raw material bamboo powder 14), and bamboo charcoal powder 16 is 800 g (lactic acid fermentation raw material bamboo. 0.08 wt% with respect to the powder 14) and the bamboo leaf powder 20 are blended at a ratio of about 10 kg (substantially the same as the lactic acid fermentation raw material bamboo powder 14).

これは、パパイン酵素等の植物酵素15が20g程度でも農作物の発根性が向上し、光合成が良好になる等、農作物の新陳代謝を促進して活性化を十分確保できるからである。また竹炭粉16を800g程度とすることにより、乳酸発酵竹粉1のpHを6±0.5程度に中性化するためである。また竹葉粉20が有する強い殺菌性、抗菌性を利用して、土壌等の殺菌性、抗菌性を高めるためである。   This is because even if the plant enzyme 15 such as papain enzyme is about 20 g, the rooting property of the crop is improved and the photosynthesis is improved, so that the metabolism of the crop can be promoted and the activation can be sufficiently secured. Further, by setting the bamboo charcoal powder 16 to about 800 g, the pH of the lactic acid fermented bamboo powder 1 is neutralized to about 6 ± 0.5. Moreover, it is for improving the bactericidal property and antibacterial property of soil etc. using the strong bactericidal property and antibacterial property which the bamboo leaf powder 20 has.

なお、圃場の土質に応じて植物酵素15は乳酸発酵原料竹粉14を10kgに対して10〜30g(乳酸発酵原料竹粉14に対して0.001〜0.003重量%)、竹炭粉16は500〜1000g(乳酸発酵原料竹粉14に対して0.05〜0.1重量%)の範囲で調整することも可能である。また乳酸発酵原料竹粉14に植物酵素15、竹炭粉16、竹葉粉20の他に米糠を配合すれば、二次発酵工程7での二次発酵を促進させることができる。   In addition, according to the soil quality of a field, the plant enzyme 15 is 10-30 g with respect to 10 kg of lactic-acid-fermentation raw material bamboo powder 14 (0.001-0.003 weight% with respect to lactic-acid-fermenting raw material bamboo powder 14), bamboo charcoal powder 16 Can be adjusted in the range of 500 to 1000 g (0.05 to 0.1% by weight based on the lactic acid fermentation raw material bamboo powder 14). If rice bran is added to the lactic acid fermentation raw material bamboo powder 14 in addition to the plant enzyme 15, bamboo charcoal powder 16 and bamboo leaf powder 20, secondary fermentation in the secondary fermentation step 7 can be promoted.

二次発酵工程7では、乳酸発酵原料竹粉14に植物酵素15と竹炭粉16と竹葉粉20との添加物を配合した配合粉を、好気性雰囲気下で2週間程度の期間をかけて二次発酵させる。この二次発酵工程7では配合粉を盛り上げた状態で二次発酵させるが、所定期間毎に攪拌、静置を繰り返しながら配合粉の全体を略均一に発酵させる。   In the secondary fermentation process 7, the blended powder in which the lactic acid fermentation raw material bamboo powder 14 is blended with the additives of plant enzyme 15, bamboo charcoal powder 16 and bamboo leaf powder 20 is applied over a period of about two weeks in an aerobic atmosphere. Secondary fermentation. In this secondary fermentation step 7, secondary fermentation is performed in a state where the blended powder is raised, but the entire blended powder is fermented substantially uniformly while repeating stirring and standing for each predetermined period.

この二次発酵工程7で配合粉を二次発酵させることにより所定の乳酸発酵竹粉1ができるので、包装工程8Aにおいて、孔19aの空いた通気性のある包装袋19に包装して最終の製品18Aとする。なお、包装袋19は密封性を有するものでもよい。   Since the predetermined lactic acid fermented bamboo powder 1 can be obtained by secondary fermentation of the blended powder in the secondary fermentation process 7, the final packaging is performed in the air-permeable packaging bag 19 having holes 19a in the packaging process 8A. Product 18A is assumed. The packaging bag 19 may have a sealing property.

このように乳酸発酵原料竹粉14に植物酵素15を配合することによって、農作物の発根性が向上し、光合成が良好になる等、農作物の新陳代謝を促進して活性化を十分確保でき、また竹炭粉16を配合することにより全体のpHを中性化する等、土壌、農作物に応じてpHを容易に調整することができる。   In this way, by blending the plant enzyme 15 with the lactic acid fermentation raw material bamboo powder 14, the rooting of the crop is improved, the photosynthesis is improved, and the metabolism of the crop is promoted to ensure sufficient activation. The pH can be easily adjusted according to the soil and crops, such as neutralizing the whole pH by blending the powder 16.

更に乳酸発酵原料竹粉14に竹葉粉20を配合しているため、この竹葉粉20により殺菌性、抗菌性が向上し、しかも乳酸発酵竹粉1の原料となる生竹9の幹の他に、その竹枝を含む竹葉までも使用でき資源の無駄を極力防止することができる。   Furthermore, since bamboo leaf powder 20 is blended with lactic acid fermentation raw material bamboo powder 14, the bamboo leaf powder 20 improves bactericidal and antibacterial properties, and the trunk of raw bamboo 9 which is the raw material of lactic acid fermentation bamboo powder 1. In addition, even bamboo leaves including the bamboo branches can be used, and waste of resources can be prevented as much as possible.

なお、この場合にも真空乳酸発酵工程4での竹粉11の一次乳酸発酵において、第1の実施形態と同様に酢酸エチルの生成を未然に防止できることは云うまでもない。   In this case as well, in the primary lactic acid fermentation of the bamboo powder 11 in the vacuum lactic acid fermentation step 4, it goes without saying that the production of ethyl acetate can be prevented as in the first embodiment.

図3は飼料用、肥料用、水産用、消臭用に用いる乳酸発酵竹液22を製造する本発明の第3の実施形態を示す。   FIG. 3 shows a third embodiment of the present invention for producing a lactic acid fermented bamboo liquid 22 used for feed, fertilizer, fisheries and deodorization.

この乳酸発酵竹液22は、乳酸発酵竹粉1にパパイン酵素等の植物酵素15、竹炭粉16等の添加物を配合し水中で発酵させてエキスを抽出したものであり、図3に示す竹粉化工程2、金属除去工程3、真空乳酸発酵工程(一次乳酸発酵工程)4、篩工程5、配合工程6、竹液発酵工程23、熟成工程24、濾過工程25、容器詰め工程26を経て製造する。   This lactic acid fermented bamboo liquid 22 is an extract obtained by blending lactic acid fermented bamboo powder 1 with additives such as plant enzyme 15 such as papain enzyme, bamboo charcoal powder 16 and the like, fermented in water, and extract the extract shown in FIG. After the powdering process 2, the metal removal process 3, the vacuum lactic acid fermentation process (primary lactic acid fermentation process) 4, the sieving process 5, the blending process 6, the bamboo liquid fermentation process 23, the aging process 24, the filtration process 25, and the container packing process 26 To manufacture.

なお、図3の製造工程では、竹粉化工程2、金属除去工程3、真空乳酸発酵工程4、篩工程5、配合工程6までは、図1に示す第1の実施形態と同じである。   In the manufacturing process of FIG. 3, the bamboo powdering process 2, the metal removing process 3, the vacuum lactic acid fermentation process 4, the sieving process 5, and the blending process 6 are the same as those in the first embodiment shown in FIG.

配合工程6では乳酸発酵原料竹粉14に植物酵素15及び竹炭粉16の添加物を配合する。竹液発酵工程23では、添加物が配合された乳酸発酵竹粉1を不織布製のフィルター27に入れた後、浸漬容器28内の活性水等の水29に浸漬する。なお、このときの乳酸発酵竹粉1と水29との割合は、乳酸発酵竹粉1が約10kgに対して水29は約80リットル程度が適当である。   In the blending step 6, the plant enzyme 15 and bamboo charcoal powder 16 additives are blended with the lactic acid fermentation raw material bamboo powder 14. In the bamboo liquid fermentation step 23, the lactic acid fermented bamboo powder 1 containing the additive is put in a non-woven filter 27 and then immersed in water 29 such as active water in an immersion container 28. In addition, the ratio of the lactic acid fermented bamboo powder 1 and the water 29 at this time is suitably about 80 liters for the water 29 while the lactic acid fermented bamboo powder 1 is about 10 kg.

そして、乳酸発酵竹粉1を水29に浸漬した後、浸漬容器28内の空間部分の空気を抜いて不活性ガス、例えば窒素ガス30に置換し、室温26°C程度の発酵室に所定期間(例えば5〜7日程度)静置して、乳酸発酵竹粉1からその二次発酵エキスである乳酸発酵竹液22の抽出と、窒素ガス雰囲気下で乳酸発酵竹粉1、乳酸発酵竹液22中の乳酸菌及び一般生菌により二次発酵とを並行して行う。   Then, after the lactic acid fermented bamboo powder 1 is immersed in the water 29, the air in the space in the immersion container 28 is evacuated and replaced with an inert gas, for example, nitrogen gas 30, and placed in a fermentation room at a room temperature of about 26 ° C. for a predetermined period. (For example, about 5 to 7 days) Leave the lactic acid fermented bamboo powder 1 as a secondary fermented extract from the lactic acid fermented bamboo powder 1, and lactic acid fermented bamboo powder 1 and lactic acid fermented bamboo liquid under a nitrogen gas atmosphere The secondary fermentation is performed in parallel with the lactic acid bacteria and general live bacteria in No. 22.

浸漬容器28は開閉蓋31を有し、乳酸発酵竹粉1を入れた状態のフィルター27を出し入れでき、また必要に応じて適宜密閉可能である。浸漬容器28にはpH検出器(図示省略)が設けられており、乳酸発酵竹液22のpHを検出できるようになっている。竹液発酵工程23の初期では、浸漬容器28内の水29はpH7程度の中性であり、これにpH4程度の乳酸発酵竹粉1を加えるため、全体としてpHが6弱程度となる。   The immersion container 28 has an open / close lid 31, and can put in and out the filter 27 in a state in which the lactic acid fermented bamboo powder 1 is put, and can be appropriately sealed as necessary. The immersion container 28 is provided with a pH detector (not shown) so that the pH of the lactic acid fermented bamboo liquid 22 can be detected. At the beginning of the bamboo liquid fermentation process 23, the water 29 in the immersion vessel 28 is neutral at about pH 7, and the lactic acid fermented bamboo powder 1 at about pH 4 is added thereto, so that the pH is about 6 as a whole.

竹液発酵工程23において乳酸菌及び一般生菌による二次発酵が進むと、浸漬容器28内のpH値が下がり、例えばpH値が5〜4.5(例えばpH4.7)になれば竹液発酵工程23での二次発酵を終了する。そして、浸漬容器28の開閉弁32を開き、浸漬容器28の乳酸発酵竹粉1から水29に溶出した二次発酵エキスである乳酸発酵竹液22を、容器33を経て又は直接熟成容器34へと取り出す。   When secondary fermentation by lactic acid bacteria and general living bacteria proceeds in the bamboo liquid fermentation process 23, the pH value in the immersion container 28 decreases. For example, if the pH value becomes 5 to 4.5 (for example, pH 4.7), the bamboo liquid fermentation is performed. The secondary fermentation in step 23 is finished. And the on-off valve 32 of the immersion container 28 is opened, and the lactic acid fermentation bamboo liquid 22 which is a secondary fermentation extract eluted from the lactic acid fermentation bamboo powder 1 of the immersion container 28 into the water 29 is passed through the container 33 or directly to the aging container 34. And take out.

熟成工程24では、抽出した乳酸発酵竹液22を熟成容器34に入れた状態で約3〜7日間程度静置して二次発酵させて熟成させる。熟成容器34内は空間部分に空気が充満する好気条件に維持する。なお、熟成容器34内も浸漬容器28と同様に不活性ガス(例えば窒素ガス)に置換してもよい。   In the aging step 24, the extracted lactic acid fermented bamboo liquid 22 is left to stand for about 3 to 7 days in a state where it is put in the aging container 34, and is subjected to secondary fermentation for aging. The inside of the aging container 34 is maintained in an aerobic condition in which the space portion is filled with air. Note that the inside of the aging container 34 may be replaced with an inert gas (for example, nitrogen gas) in the same manner as the immersion container 28.

この熟成工程24での熟成期間中に異常がなければ、濾過工程25にて熟成容器34の乳酸発酵竹液22を濾過して酵母の膜やゴミ等を除去した後、容器詰め工程26で乳酸発酵竹液22を適当な容器35に詰めて最終の製品36とする。乳酸発酵竹液22の品質に異常があれば、熟成工程24での熟成期間中にその異常が顕著になるので、その時点で廃棄その他の適宜措置を講じる。なお、竹液発酵工程23と熟成工程24は、同一容器内で連続して行うようにしてもよい。   If there is no abnormality during the ripening period in the ripening step 24, the lactic acid fermented bamboo liquid 22 in the ripening vessel 34 is filtered in the filtration step 25 to remove yeast membranes and dust, and then the lactic acid in the container filling step 26. The fermented bamboo liquid 22 is packed in a suitable container 35 to obtain a final product 36. If there is an abnormality in the quality of the lactic acid fermented bamboo liquid 22, the abnormality becomes remarkable during the aging period in the aging process 24, and disposal or other appropriate measures are taken at that time. In addition, you may make it perform the bamboo liquid fermentation process 23 and the aging process 24 continuously in the same container.

このようにして製造した乳酸発酵竹液22は、飼料用、肥料用、水産用、消臭用の何れに利用する場合にも、従来の乳酸発酵竹粉1に比較して嵩張りがなく取り扱いが容易であり、用途に応じて容易に活用でき利便性が著しく向上する。例えば、乳牛等の家畜の飼料として粗飼料に配合して給餌する場合にも、乳酸発酵竹液22を粗飼料に容易に混ぜ合わせることができ、従来の竹繊維による問題等を解消できる。   The lactic acid fermented bamboo liquid 22 produced in this way is handled with less bulk compared to the conventional lactic acid fermented bamboo powder 1 when used for feed, fertilizer, aquaculture, or deodorization. It can be easily used according to the application, and the convenience is remarkably improved. For example, even when blended and fed into roughage as feed for livestock such as dairy cows, the lactic acid fermented bamboo liquid 22 can be easily mixed with the roughage, and problems with conventional bamboo fibers can be solved.

大気圧下で一次乳酸発酵させて得た乳酸発酵竹粉(試料1)と、各実施形態での真空乳酸発酵工程4において真空下で一次乳酸発酵させて得た乳酸発酵竹粉1の6種類の試料2〜7について、気圧、pH、一般生菌、酢酸エチルの関係を分析したところ、図4に示すような結果が得られた。   Six types of lactic acid fermented bamboo powder (sample 1) obtained by primary lactic acid fermentation under atmospheric pressure and lactic acid fermented bamboo powder 1 obtained by primary lactic acid fermentation under vacuum in vacuum lactic acid fermentation step 4 in each embodiment. Samples 2 to 7 were analyzed for the relationship among atmospheric pressure, pH, general viable bacteria, and ethyl acetate. The results shown in FIG. 4 were obtained.

この分析結果を見る限りは、大気圧下で乳酸発酵させた試料1では、45mg/100g程度の微量の酢酸エチルの生成が検出されたが、真空下で乳酸発酵させた試料2〜7では、乳酸発酵竹粉1中の酢酸エチルの生成は検出されなかった。従って、竹粉11を乳酸発酵させて乳酸発酵竹粉1を製造するに当たっては、真空容器41内を真空状態に維持して、その真空下で竹粉11を乳酸発酵させることにより、酢酸エチルの生成を防止できることが確認できた。   As far as this analysis result is seen, in Sample 1 fermented with lactic acid under atmospheric pressure, a trace amount of ethyl acetate of about 45 mg / 100 g was detected, but in Samples 2 to 7 fermented with lactic acid under vacuum, Production of ethyl acetate in lactic acid fermented bamboo powder 1 was not detected. Therefore, in producing the lactic acid fermented bamboo powder 1 by fermenting the bamboo powder 11 with lactic acid, the inside of the vacuum vessel 41 is maintained in a vacuum state, and the bamboo powder 11 is subjected to lactic acid fermentation under the vacuum, thereby producing ethyl acetate. It was confirmed that generation could be prevented.

従って、真空乳酸発酵工程4において真空容器41内を低真空状態に維持しておけば、乳酸発酵竹粉1中の酢酸エチルの生成を防止することが可能である。しかし、酢酸エチルのない乳酸発酵竹粉1を安定的に製造するためには、中真空以上であることが望ましい。   Therefore, if the inside of the vacuum vessel 41 is maintained in a low vacuum state in the vacuum lactic acid fermentation step 4, it is possible to prevent the production of ethyl acetate in the lactic acid fermentation bamboo powder 1. However, in order to stably produce the lactic acid fermented bamboo powder 1 free of ethyl acetate, it is desirable that the pressure be medium vacuum or higher.

図4の気圧は大気圧を0.00とし、試料2〜7の製造に供した真空装置40の最大真空度を0.10として、大気圧0.00と真空度0.10との間を10段階に分けた上で、その試料2〜7を製造したときの各段階の気圧を各試料2〜7毎に示すものである。この図4に示すように、真空乳酸発酵工程4での真空容器41内の真空度が高くなる程、乳酸発酵後の乳酸発酵竹粉1のpH値が低くなり、一般生菌数が増える傾向にある。   The atmospheric pressure in FIG. 4 is set to 0.00, the maximum vacuum degree of the vacuum apparatus 40 used for the manufacture of the samples 2 to 7 is 0.10, and between the atmospheric pressure 0.00 and the vacuum degree 0.10. After dividing into 10 stages, the atmospheric pressure at each stage when the samples 2 to 7 are manufactured is shown for each sample 2 to 7. As shown in FIG. 4, the higher the degree of vacuum in the vacuum vessel 41 in the vacuum lactic acid fermentation step 4, the lower the pH value of the lactic acid fermented bamboo powder 1 after lactic acid fermentation, and the number of general viable bacteria increases. It is in.

従って、図3の第3実施形態に例示するように、竹液発酵工程23において乳酸発酵竹粉1を水29に浸漬して二次発酵させるような場合には、その二次発酵を考慮して乳酸菌を含む一般生菌数が多く、しかも真空度の維持コストも安い試料6のpH4.76程度で一次乳酸発酵の終了とするのが適当である。   Therefore, as illustrated in the third embodiment of FIG. 3, in the case where the lactic acid fermented bamboo powder 1 is immersed in the water 29 and subjected to secondary fermentation in the bamboo liquid fermentation step 23, the secondary fermentation is considered. Thus, it is appropriate to end primary lactic acid fermentation at about pH 4.76 of sample 6 having a large number of general viable bacteria containing lactic acid bacteria and low vacuum maintenance cost.

なお、乳酸発酵竹粉1の用途によっては、試料2〜5の何れかのpH値を以て一次乳酸発酵の終了としてもよい。   In addition, depending on the use of the lactic acid fermentation bamboo powder 1, it is good also as completion | finish of primary lactic acid fermentation with the pH value in any one of the samples 2-5.

また竹粉11を一次乳酸発酵させて乳酸発酵原料竹粉14とし、この乳酸発酵原料竹粉14に発酵促進効果を有する植物酵素15を配合して二次発酵させることにより、一次乳酸発酵と二次発酵とで発酵条件を変えることができ、真空乳酸発酵工程4とその後の竹液発酵工程23とで生成される乳酸発酵菌を異ならせることが可能である。   Moreover, the primary lactic acid fermentation and the second are obtained by subjecting the bamboo powder 11 to primary lactic acid fermentation to obtain a lactic acid fermentation raw material bamboo powder 14, and by adding a plant enzyme 15 having a fermentation promoting effect to the lactic acid fermentation raw material bamboo powder 14 and performing secondary fermentation. The fermentation conditions can be changed in the subsequent fermentation, and the lactic acid fermentation bacteria produced in the vacuum lactic acid fermentation process 4 and the subsequent bamboo liquid fermentation process 23 can be made different.

例えば、竹粉11の一次乳酸発酵では、その乳酸発酵原料竹粉14中に乳酸菌ラクトコッカスを生成させて、またその乳酸発酵原料竹粉14にパパイン酵素等の植物酵素15を配合することにより、その後の竹液発酵工程23、熟成工程24での二次発酵により、バクトバチルスブレビス及びラクトバチルスカゼイ等の嫌気性の乳酸菌と、アセトバクター等の好気性の酢酸菌とを生成させることができる。従って、乳酸発酵竹液22は有用性のある多種類の乳酸菌、酢酸菌等を含むものとなり、それ自体の用途を多方面に展開できる利点がある。   For example, in the primary lactic acid fermentation of bamboo powder 11, by producing lactic acid bacteria Lactococcus in the lactic acid fermentation raw material bamboo powder 14 and blending the lactic acid fermentation raw material bamboo powder 14 with plant enzymes 15 such as papain enzyme, Subsequent fermentation in the bamboo liquid fermentation step 23 and the ripening step 24 can generate anaerobic lactic acid bacteria such as Bactobacillus brevis and Lactobacillus casei and aerobic acetic acid bacteria such as Acetobacter. Therefore, the lactic acid fermented bamboo liquid 22 contains many kinds of useful lactic acid bacteria, acetic acid bacteria, and the like, and has an advantage that its own uses can be developed in various fields.

なお、変性剤濃度勾配ゲル電気泳動法(Denaturing Gradient Gel Electrophoresis : DGGE)により、竹液発酵工程23を経た後に抽出した二次発酵エキスである乳酸発酵竹液22の試料(例えば、発酵開始7日目のもの)について解析したところ、嫌気性の乳酸菌(例えばラクトバチルス属(Lactobacillus brevis、Lactobacillus coryniformis、Lactobacillus casei 、Lactobacillus paracasei subsp.paracasei 、Lactobacillus paracollinoides 、))のDNAバンドが検出された。またDGGEにより、熟成工程24終了後の乳酸発酵竹液22の試料を解析したところ、好気性の酢酸菌(例えばアセトバクター・パスツリアナス(Acetobacter pasteurianus))のDNAバンドが検出された。   A sample of lactic acid fermentation bamboo liquor 22, which is a secondary fermentation extract extracted after passing through the bamboo liquor fermentation step 23 by denaturing gradient gel electrophoresis (DGGE) (for example, 7 days from the start of fermentation) As a result of analysis on the eyes, DNA bands of anaerobic lactic acid bacteria (for example, Lactobacillus brevis, Lactobacillus coryniformis, Lactobacillus casei, Lactobacillus paracasei subsp. Paracasei, Lactobacillus paracollinoides) were detected. Further, when a sample of the lactic acid fermented bamboo liquid 22 after completion of the ripening step 24 was analyzed by DGGE, a DNA band of an aerobic acetic acid bacterium (for example, Acetobacter pasteurianus) was detected.

これは窒素ガス置換の竹液発酵工程23では嫌気性の乳酸菌による乳酸発酵があり、好気条件下の熟成工程24では好気性の酢酸菌による熟成発酵があったと推測される。   This is presumed that there was lactic acid fermentation by anaerobic lactic acid bacteria in the bamboo liquid fermentation process 23 with nitrogen gas substitution, and aging fermentation by aerobic acetic acid bacteria in the aging process 24 under aerobic conditions.

このようにして製造した乳酸発酵竹液22は水で適当に希釈化して、家畜の飼料、飼料用の添加剤、肥料用又は水産用として使用することが可能であり、乳酸発酵竹粉1に比較して遥かに優れた有用性を期待することができる。   The lactic acid fermented bamboo liquid 22 produced in this manner can be appropriately diluted with water and used for livestock feed, feed additives, fertilizers or aquatic products. Compared with this, much better utility can be expected.

なお、この第3の実施形態においても、配合工程6において第2の実施形態と同様に竹葉粉20を配合するようにしてもよい。   In the third embodiment, the bamboo leaf powder 20 may be blended in the blending step 6 as in the second embodiment.

図5は食品用、化粧品用、医薬部外品及び医薬品に用いる乳酸発酵竹液22を製造する本発明の第4の実施形態を示す。   FIG. 5 shows a fourth embodiment of the present invention for producing a lactic acid fermented bamboo liquid 22 used for foods, cosmetics, quasi drugs and pharmaceuticals.

この実施形態の乳酸発酵竹液22は、乳酸発酵原料竹粉14に対して、pH7未満の酸性を有する酸性質植物37、取り分け天然果実であるボイセンベリーと、笹を切削又は粉砕した粉状の竹葉粉(笹粉)12と、竹根を切削又は粉砕した粉状の竹根粉13とを配合した後、それらが配合された乳酸発酵竹粉1をフィルター27に入れて水29中に浸漬し二次発酵させてエキス(二次発酵エキス)を抽出したものである。   The lactic acid fermented bamboo liquid 22 of this embodiment is a powdery product obtained by cutting or pulverizing the lactic acid fermentation raw material bamboo powder 14 with acidity plant 37 having an acidity of less than pH 7, especially Boysenberry, which is a natural fruit, and straw. After blending bamboo leaf powder (bamboo powder) 12 and powdered bamboo root powder 13 obtained by cutting or pulverizing bamboo root, lactic acid fermented bamboo powder 1 blended with them is put in filter 27 and put in water 29. The extract (secondary fermentation extract) is extracted by dipping and secondary fermentation.

この乳酸発酵竹液22の製造は、配合工程6において、乳酸発酵原料竹粉14(例えば5kg)に対して、乳酸発酵原料竹粉14と略同じ5kgの竹葉粉12と、2kgの竹根粉13と、1.2kgの酸性質植物37のボイセンベリーとを配合し混合している点で第3の実施形態と相違する。   In the blending step 6, the lactic acid fermented bamboo liquid 22 is produced in the blending process 6 with respect to the lactic acid fermented raw material bamboo powder 14 (for example, 5 kg). It is different from the third embodiment in that the powder 13 and 1.2 kg of the Boysenberry of the acidity plant 37 are blended and mixed.

このように乳酸発酵原料竹粉14に竹葉粉12、竹根粉13及びボイセンペリーを所定の割合で配合した配合粉を水29に浸漬し二次発酵させた後に、そのエキスである二次発酵エキスを抽出することにより、例えば図6に示すように乳酸2300μg/mL、酢酸610μg/mLを代表とする有機酸を含み、且つ図7に示すように多種類のアミノ酸を含む乳酸発酵竹液22を製造することができる。   Thus, the secondary powder which is the extract is obtained by immersing the compounded powder in which the bamboo leaf powder 12, the bamboo root powder 13 and the Boysen Perry are blended at a predetermined ratio into the lactic acid fermentation raw material bamboo powder 14 in water 29 and performing secondary fermentation. By extracting the fermented extract, for example, as shown in FIG. 6, a lactic acid fermented bamboo liquid containing an organic acid typified by 2300 μg / mL lactic acid and 610 μg / mL acetic acid and containing various kinds of amino acids as shown in FIG. 22 can be manufactured.

なお、竹葉粉12は乳酸発酵原料竹粉14と略同じ重量を配合しているが、乳酸発酵原料竹粉14よりも若干少ないか、又は若干多くしてもよい。従って、竹葉粉12は乳酸発酵原料竹粉14に対して85〜120重量%、望ましくは90〜110重量%が適当である。また竹根粉13は乳酸発酵原料竹粉14の約40重量%内外、例えば30〜50重量%程度が適当である。酸性質植物37のボイセンベリーは、乳酸発酵原料竹粉14と竹葉粉12と竹根粉13との総重量の10〜12重量%内外、例えば8〜15重量%程度が適当である。   The bamboo leaf powder 12 contains approximately the same weight as the lactic acid fermentation raw material bamboo powder 14, but may be slightly less than or slightly larger than the lactic acid fermentation raw material bamboo powder 14. Therefore, the bamboo leaf powder 12 is 85 to 120% by weight, preferably 90 to 110% by weight, based on the lactic acid fermentation raw material bamboo powder 14. The bamboo root powder 13 is suitably about 40% by weight or more, for example, about 30 to 50% by weight of the lactic acid fermentation raw material bamboo powder 14. The Boysenberry of the acid property plant 37 is suitably 10 to 12% by weight, for example, about 8 to 15% by weight of the total weight of the lactic acid fermentation raw material bamboo powder 14, bamboo leaf powder 12 and bamboo root powder 13.

ボイセンベリーは例えば乾燥後に切削又は粉砕等した粉状のものが挙げられる。ボイセンベリーは、ポリフェノールの一種アントシアニン、葉酸、エラグ酸など優れた抗酸化作用を示す栄養素や、ミネラル(カルシウムや鉄分)が含まれているため、ボイセンベリーのアントシアニン等の抗酸化物質により活性酸素の生成を抑制することができ、ミネラルを摂取することができる。   Boysen berries include, for example, powders that are cut or crushed after drying. Boysenberry contains nutrients that exhibit excellent antioxidant action such as anthocyanin, folic acid, and ellagic acid, and minerals (calcium and iron), and therefore, active oxygen is absorbed by antioxidants such as anthocyanins in Boysenberry. Production can be suppressed and minerals can be ingested.

竹葉粉12は多量の大腸菌を含む一般生菌を有し、これを配合することにより竹液発酵工程23、熟成工程24での二次発酵を促進させることができる。また竹根粉13は優れた殺菌性・抗菌性を有する。   Bamboo leaf powder 12 has general living bacteria containing a large amount of Escherichia coli, and by blending this, secondary fermentation in bamboo liquid fermentation process 23 and ripening process 24 can be promoted. Bamboo root powder 13 has excellent bactericidal and antibacterial properties.

竹液発酵工程23では、配合工程6でボイセンベリー等が配合された後の乳酸発酵竹粉1(約13kg)を入れた不織布製のフィルター27を、活性水等の水29を約100リットル入れた浸漬容器28内に浸漬し、その状態で第3の実施形態と同様に発酵室にて室温26°C程度で所定期間(例えば10日間)静置して二次発酵させる。   In the bamboo liquid fermentation process 23, a non-woven filter 27 containing the lactic acid fermented bamboo powder 1 (about 13 kg) after mixing the boysenberry in the mixing process 6 is added, and about 100 liters of water 29 such as active water is added. Then, it is immersed in the dipping vessel 28, and in this state, it is left to stand at a room temperature of about 26 ° C. for a predetermined period (for example, 10 days) in the fermentation room to perform secondary fermentation.

熟成工程24では、第3の実施形態と同様に乳酸発酵竹粉1から抽出した乳酸発酵竹液22を熟成容器34に入れた状態で約3日間程度静置して熟成させる。なお、熟成工程24で熟成後の乳酸発酵竹液22は、図5の破線矢印で示すように容器詰め工程26Aにおいて適当な容器35に詰めて最終の製品36としてもよい。なお、この製品36は、畜産及びペット用飲料添加液としての用途のものである。   In the aging step 24, as in the third embodiment, the lactic acid fermented bamboo liquid 22 extracted from the lactic acid fermented bamboo powder 1 is left in the aging container 34 for about 3 days to be aged. Note that the lactic acid fermented bamboo liquid 22 after ripening in the ripening step 24 may be packed in an appropriate container 35 in the container filling step 26A as shown by a broken line arrow in FIG. In addition, this product 36 is a thing of the use as livestock and a drink additive liquid for pets.

また熟成工程24で熟成し濾過工程25で濾過後の乳酸発酵竹液22をオートクレーブ滅菌工程38で滅菌してもよい。例えばオートクレーブ滅菌装置において、120°C以上において高圧(例えば2気圧)で所定時間(例えば30分間)にわたる高圧滅菌処理を行う。この滅菌処理を行うことで乳酸発酵竹液22中の乳酸菌や酵母菌などの菌が死滅し、酵素(アミノ酸)などを残すことができ、食品用、化粧品用、医薬部外品及び医薬品の乳酸発酵竹液22の提供が可能となる。   Alternatively, the lactic acid fermented bamboo liquid 22 ripened in the ripening step 24 and filtered in the filtration step 25 may be sterilized in the autoclave sterilization step 38. For example, in an autoclave sterilizer, high-pressure sterilization is performed at a high pressure (eg, 2 atm) at 120 ° C. or higher for a predetermined time (eg, 30 minutes). By performing this sterilization treatment, bacteria such as lactic acid bacteria and yeast in the lactic acid fermented bamboo liquid 22 can be killed, leaving enzymes (amino acids), etc., and lactic acid for foods, cosmetics, quasi drugs and pharmaceuticals. The fermented bamboo liquid 22 can be provided.

このオートクレーブ滅菌工程38で滅菌処理後の乳酸発酵竹液22を、容器詰め工程26において適当な容器35に詰めて最終の製品36とする。またオートクレーブ滅菌工程38で滅菌処理後の乳酸発酵竹液22を機能水(例えばセラミック水(セラミックに接触させた水))で適当に希釈化してから、容器詰め工程26において適当な容器35に詰めて最終の製品36としてもよい。この製品36は、食品用、化粧品用、医薬部外品及び医薬品等の乳酸発酵竹液22であり、例えば食品添加液(食品用旨み成分向上液など)、化粧品(肌水や保湿ジェルなど)、抗菌液等の用途が挙げられる。   The lactic acid fermented bamboo liquid 22 that has been sterilized in the autoclave sterilization process 38 is packed in a suitable container 35 in the container packing process 26 to obtain a final product 36. The lactic acid fermented bamboo liquid 22 sterilized in the autoclave sterilization process 38 is appropriately diluted with functional water (for example, ceramic water (water in contact with the ceramic)) and then packed in a suitable container 35 in the container packing process 26. The final product 36 may be used. This product 36 is a lactic acid fermented bamboo liquid 22 for foods, cosmetics, quasi-drugs, pharmaceuticals, etc., for example, food additive liquids (such as umami ingredient improving liquids for foods), cosmetics (such as skin water and moisturizing gel). And uses such as an antibacterial liquid.

この第4の実施形態で例示の2種類の乳酸発酵竹液22、つまり、オートクレーブ滅菌工程38を経た製品36(例えば食品用、化粧品用、医薬部外品及び医薬品等の乳酸発酵竹液22)である製品Aと、熟成工程24後の製品36(例えば畜産及びペット用飲料添加液)である製品Bは、第3の実施形態での製品36である製品Cと比べて、殺菌性・抗菌性が顕著に優れた効果を有する。   The two types of lactic acid fermented bamboo liquid 22 exemplified in the fourth embodiment, that is, a product 36 that has undergone an autoclave sterilization step 38 (for example, lactic acid fermented bamboo liquid 22 for food, cosmetics, quasi drugs, pharmaceuticals, etc.) The product A which is the product A and the product 36 after the ripening step 24 (for example, livestock and pet beverage additive liquid) are more bactericidal and antibacterial than the product C which is the product 36 in the third embodiment. The effect is remarkably excellent.

製品A、Cに関して、大腸菌(血清O−157:H7)に対する抗菌力の試験を行った。まず、大腸菌6.2×105 μg/mLを培養した培地に製品Cの試験液1mLを投与すると、1時間後の大腸菌が3.7×105 μg/mLとなり、2時間後の大腸菌が8.4×104 μg/mLとなって、4時間後に大腸菌が死滅した。   The products A and C were tested for antibacterial activity against E. coli (serum O-157: H7). First, when 1 mL of the test solution of product C is administered to a medium in which 6.2 × 10 5 μg / mL of E. coli is cultured, E. coli after 1 hour becomes 3.7 × 10 5 μg / mL, and E. coli after 2 hours becomes 8. At 4 × 10 4 μg / mL, E. coli was killed after 4 hours.

なお、大腸菌6.2×105 μg/mLの培地にボイセンベリー単体の抽出液の試験液1mLを投与した場合には、6時間後にも1.6×105 μg/mLの大腸菌が確認され、大腸菌は死滅しなかった。   In addition, when 1 mL of a test solution of Boysenberry extract was administered to a medium of 6.2 × 10 5 μg / mL of E. coli, 1.6 × 10 5 μg / mL of E. coli was confirmed after 6 hours. Did not die.

これに対して、大腸菌6.1×105 μg/mLの培地に製品Aの試験液1mLを投与すると、30分後に大腸菌が20μg/mLとなり、1時間後に大腸菌が死滅した。この抗菌力試験の結果から、製品Aには大腸菌を死滅させる顕著な抗菌効果を有することが分かった。   On the other hand, when 1 mL of the test solution of product A was administered to the medium of E. coli 6.1 × 10 5 μg / mL, E. coli was 20 μg / mL after 30 minutes, and E. coli was killed after 1 hour. From the results of this antibacterial activity test, it was found that Product A has a remarkable antibacterial effect that kills E. coli.

また製品Bに関して、カンピロバクターに対する抗菌力の試験を行った。カンピロバクター1.7×105 μg/mLを培養した培地に製品Bの試験液1mLを投与したところ、10分後にカンピロバクターが300μg/mLとなり、30分後にカンピロバクターが死滅した。同様にカンピロバクター1.7×105 μg/mLの培地に製品Bの5倍希釈した試験液1mLを投与すると、30分後にカンピロバクターが400μg/mLとなった。この抗菌力試験の結果から、製品Bにはカンピロバクターを死滅させる顕著な抗菌効果を有することが分かった。   Further, the product B was tested for antibacterial activity against Campylobacter. When 1 mL of the test solution of product B was administered to the medium in which Campylobacter 1.7 × 10 5 μg / mL was cultured, Campylobacter became 300 μg / mL after 10 minutes, and Campylobacter died after 30 minutes. Similarly, when 1 mL of a test solution diluted 5-fold of product B was administered to a medium of Campylobacter 1.7 × 10 5 μg / mL, Campylobacter became 400 μg / mL after 30 minutes. From the results of this antibacterial activity test, it was found that Product B has a remarkable antibacterial effect that kills Campylobacter.

また製品A〜Cについてのアミノ酸測定を行った結果、必須アミノ酸であるフェニルアラニンは、製品Cでは2.8μg/mL程度であるのに対して、製品Bでは420μg/mL、製品Aでは470μg/mLと格段に多いことが分かった。このフェニルアラニンは鎮痛効果が高く、慢性的な痛みを軽減する効果を有するものである。   In addition, as a result of amino acid measurement on products A to C, phenylalanine, which is an essential amino acid, is about 2.8 μg / mL in product C, whereas 420 μg / mL in product B and 470 μg / mL in product A. It turned out to be much more. This phenylalanine has a high analgesic effect and has an effect of reducing chronic pain.

例えば、オートクレーブ滅菌工程34を経た製品36を食品用等の乳酸発酵竹液22として使用する場合には、ボイセンベリー由来の抗酸化作用と竹根粉及び竹葉粉由来の殺菌作用により、抗菌液として使用できる他に、含有するアミノ酸により食品の旨み成分を高めることができ、また天然の防腐剤としての利点がある。   For example, when the product 36 that has undergone the autoclave sterilization step 34 is used as the lactic acid fermented bamboo liquid 22 for foods or the like, the antibacterial liquid is obtained by the antioxidative action derived from Boysenberry and the bactericidal action derived from bamboo root powder and bamboo leaf powder. In addition to being able to be used as an amino acid, it is possible to enhance the umami component of foods, and there is an advantage as a natural preservative.

図8は食品用、化粧品用、医薬部外品及び医薬品に用いる乳酸発酵竹液22を製造する第5の実施形態を示す。   FIG. 8 shows a fifth embodiment for producing a lactic acid fermented bamboo liquid 22 used for foods, cosmetics, quasi drugs and pharmaceuticals.

この実施形態は、乳酸発酵原料竹粉14、竹葉粉12、竹根粉13の割合を第4の実施形態と同じとし、ボイセンベリーの配合量を乳酸発酵原料竹粉14、竹葉粉12及び竹根粉13の総重量の1〜3重量%の範囲内の300gに抑えて、乳酸発酵竹液22を製造する点において、第4の実施形態と相違している。   In this embodiment, the proportions of lactic acid fermentation raw material bamboo powder 14, bamboo leaf powder 12 and bamboo root powder 13 are the same as those in the fourth embodiment, and the amount of Boysenberry is blended in lactic acid fermentation raw material bamboo powder 14 and bamboo leaf powder 12 And it is different from 4th Embodiment in the point which restrains to 300 g in the range of 1-3 weight% of the total weight of the bamboo root powder 13, and manufactures the lactic acid fermentation bamboo liquid 22. FIG.

なお、ボイセンベリーの配合量は300gであり、乳酸発酵原料竹粉14、竹葉粉12及び竹根粉13の総重量の2.5重量%としているが、1〜3重量%であればよい。   The blended amount of Boysenberry is 300 g and is 2.5% by weight of the total weight of the lactic acid fermentation raw material bamboo powder 14, bamboo leaf powder 12 and bamboo root powder 13, but it may be 1 to 3% by weight. .

このような割合で乳酸発酵原料竹粉14にボイセンベリーと竹葉粉12と竹根粉13とを配合して配合粉とした後、その配合粉を水29中に浸漬し二次発酵させてエキス(二次発酵エキス)を抽出することによって、例えば図9に示すように乳酸3000μg/mL、酢酸930μg/mLを代表とする有機酸を含み、且つ図10に示すように多種類のアミノ酸を含む乳酸発酵竹液22を製造することができる。   After blending Boysenberry, bamboo leaf powder 12 and bamboo root powder 13 into the lactic acid fermentation raw material bamboo powder 14 at such a ratio to make a blended powder, the blended powder is immersed in water 29 for secondary fermentation. By extracting the extract (secondary fermentation extract), for example, as shown in FIG. 9, it contains an organic acid typified by lactic acid 3000 μg / mL and acetic acid 930 μg / mL, and as shown in FIG. The lactic acid fermented bamboo liquid 22 containing can be manufactured.

第4の実施形態と第5の実施形態では、乳酸発酵原料竹粉14、竹葉粉12及び竹根粉13の配合量は同一であるが、ボイセンベリーの配合量が大きく異なっている。第4の実施形態の場合には、ボイセンベリーを1.2Kgと多く配合しているため、ボイセンベリーの配合量の増加が乳酸発酵竹液中の多量のアミノ酸の生成に大きく寄与し、旨み成分が向上する結果が得られた。従って、第4の実施形態の乳酸発酵竹液22は、旨み向上用の食品添加物として活用することが可能である。   In the fourth embodiment and the fifth embodiment, the blending amounts of the lactic acid fermentation raw material bamboo powder 14, the bamboo leaf powder 12 and the bamboo root powder 13 are the same, but the blending amounts of Boysenberry are greatly different. In the case of the fourth embodiment, since a large amount of Boysenberry is blended with 1.2 kg, the increase in the amount of Boysenberry greatly contributes to the production of a large amount of amino acids in the lactic acid fermentation bamboo liquid, and the umami component Improved results were obtained. Therefore, the lactic acid fermented bamboo liquid 22 of the fourth embodiment can be used as a food additive for improving umami.

一方、ボイセンベリーの配合量の増加によって、有機酸の乳酸が2300μg/mL、酢酸が280μg/mL程度となり、大腸菌、カンピロバクターに対する殺菌性を有するものの、黄色ブドウ球菌等の一部の細菌に対する殺菌性が低下する結果が得られた。   On the other hand, with the increase in the amount of Boysenberry, the organic acid lactic acid is about 2300 μg / mL and acetic acid is about 280 μg / mL, and it has bactericidal properties against Escherichia coli and Campylobacter, but it also has bactericidal properties against some bacteria such as Staphylococcus aureus. The result of decreasing was obtained.

これに対して第5の実施形態に示すように、ボイセンベリーの配合量を乳酸発酵原料竹粉14、竹葉粉12及び竹根粉13の総重量の3重量%以下の2.5重量%程度まで少なくして第4の実施形態と同様の方法で乳酸発酵竹液22を製造し、その乳酸発酵竹液22を分析したところ、図9に示すように有機酸の乳酸が3000μg/mL、酢酸が900μg/mLと増加し、また図10に示すように多種類のアミノ酸を有する結果が得られた。   On the other hand, as shown in the fifth embodiment, the amount of Boysenberry is 2.5% by weight, which is 3% by weight or less of the total weight of the lactic acid fermentation raw material bamboo powder 14, bamboo leaf powder 12 and bamboo root powder 13. The lactic acid fermented bamboo liquid 22 was produced in the same manner as in the fourth embodiment with a reduced amount to the extent, and the lactic acid fermented bamboo liquid 22 was analyzed. As shown in FIG. 9, the organic acid lactic acid was 3000 μg / mL, Acetic acid was increased to 900 μg / mL, and as shown in FIG. 10, a result having many kinds of amino acids was obtained.

そして、この乳酸発酵竹液22を黄色ブドウ球菌の培地に投与して殺菌性を調べたところ、黄色ブドウ球菌に対する殺菌性が確認できた。勿論、第4の実施形態の場合と同様に大腸菌、カンピロバクターに対する殺菌性も確認できた。   And when this lactic-acid-fermented bamboo liquid 22 was administered to the culture medium of Staphylococcus aureus and the bactericidal property was investigated, the bactericidal property with respect to Staphylococcus aureus was confirmed. Of course, as in the case of the fourth embodiment, bactericidal properties against Escherichia coli and Campylobacter were also confirmed.

従って、ボイセンベリーの配合量を乳酸発酵原料竹粉14、竹葉粉12及び竹根粉13の総重量の3重量%以下にして製造した乳酸発酵竹液22は、旨み成分の元であるアミノ酸の点では第4の実施形態の場合に比較して若干低下するものの、殺菌性の点で非常に優れており、広く殺菌剤として活用することが可能である。   Therefore, the lactic acid fermented bamboo liquid 22 produced with the blended amount of Boysenberry with 3% by weight or less of the total weight of the lactic acid fermentation raw material bamboo powder 14, bamboo leaf powder 12 and bamboo root powder 13 is an amino acid that is the source of the umami component. In this respect, although slightly lower than in the case of the fourth embodiment, it is very excellent in terms of bactericidal properties, and can be widely used as a bactericidal agent.

以上、本発明の各実施形態について詳述したが、本発明は各実施形態に限定されるものではなく、本発明の趣旨を逸脱しない範囲で種々の変更が可能である。   As mentioned above, although each embodiment of this invention was explained in full detail, this invention is not limited to each embodiment, A various change is possible in the range which does not deviate from the meaning of this invention.

例えば、各実施形態では、乳酸発酵原料竹粉14、植物酵素15、竹炭粉16、竹葉粉20、竹根粉13を配合する場合の具体的な割合を示しているが、これは一応の目安であって、飼料用、肥料用、水産用、食品用、化粧品用、医薬部外品及び医薬品の何れの場合にも、その対象とする家畜の種類、土壌の性質、農作物の種類、食品の種類、化粧品の種類、医薬部外品及び医薬品の種類等に応じて種々の組み合わせがあり、例示の数値に限定されるものではない。   For example, in each embodiment, the specific ratio in the case of blending the lactic acid fermentation raw material bamboo powder 14, the plant enzyme 15, the bamboo charcoal powder 16, the bamboo leaf powder 20, and the bamboo root powder 13 is shown. In the case of feed, fertilizer, aquaculture, food, cosmetics, quasi-drugs, and pharmaceuticals, the target species of livestock, soil properties, crop types, food There are various combinations depending on the type of cosmetic, the type of cosmetic, the quasi-drug, the type of pharmaceutical, and the like, and are not limited to the exemplified numerical values.

また竹枝を含む竹葉を粉砕したものを竹葉粉20として例示しているが、竹枝を含まず笹を粉状にした笹粉でもよい。また竹葉粉20は孟宗竹以外の竹葉、例えば真竹の竹葉、熊笹その他の笹の葉等を利用することが可能である。従って、竹葉粉は笹を含む広義の竹類の竹葉を粉状にしたものであれば十分である。原料の生竹9は孟宗竹が一般的であるが、孟宗竹以外のものでもよい。   Moreover, although the thing which grind | pulverized the bamboo leaf containing a bamboo branch is illustrated as the bamboo leaf powder 20, the powdered rice cake which does not contain a bamboo branch and is made into a powder form may be sufficient. Further, the bamboo leaf powder 20 can use bamboo leaves other than 孟宗竹, such as bamboo leaves of true bamboo, bear leaves and other bamboo leaves. Accordingly, it is sufficient that the bamboo leaf powder is a powder of bamboo leaves in a broad sense including bamboo shoots. The raw raw bamboo 9 is generally 孟宗竹, but may be other than 孟 孟 bamboo.

各実施形態では、配合工程6で植物酵素15としてパパイン酵素を、酸性質植物37としてボイセンベリーを夫々配合する場合を例示しているが、パパイン酵素以外の植物酵素15でもよいし、ボイセンベリー以外の酸性質植物37でもよい。   In each embodiment, the case where papain enzyme is blended as the plant enzyme 15 and boysenberry is blended as the acidity plant 37 in the blending step 6 is exemplified, but the plant enzyme 15 other than the papain enzyme may be used, or other than boysenberry. The acidity plant 37 may be used.

例えば、酸性質植物37には、ボイセンベリーを含むブルーベリー、ストロベリー等のベリー類、みかん、レモン等の柑橘類、梅、その他の果実類が適当である。しかし、pHが7未満の酸性を有する天然植物であれば、果実類以外の葉物野菜その他の野菜類、草類等も使用可能である。また酸性質植物37には、天然植物を原料とする加工野菜、加工果実等の加工植物類も使用可能である。なお、これらの酸性質植物37はpHが5又は4未満程度のものが望ましい。   For example, blueberries including Boysenberry, berries such as strawberry, citrus fruits such as mandarin orange and lemon, plums, and other fruits are suitable for the acidic plant 37. However, leafy vegetables other than fruits, other vegetables, grasses, and the like can be used as long as they are natural plants having an acidic pH of less than 7. In addition, as the acidic plant 37, processed plants such as processed vegetables and processed fruits made from natural plants can be used. These acidic plants 37 preferably have a pH of about 5 or less than 4.

また各実施形態において、植物酵素15に代えて酸性質植物37を、酸性質植物37に代えて植物酵素15を夫々用いてもよい。従って、第1、第2の実施形態の乳酸発酵竹粉1の製造に際しても、植物酵素15に代えてボイセンベリー等の酸性質植物37を配合することも可能である。植物酵素15、酸性質植物37は粉状、顆粒状で配合するのが望ましいが、細片状、その他の状態で配合してもよい。   In each embodiment, the acidity plant 37 may be used instead of the plant enzyme 15, and the plant enzyme 15 may be used instead of the acidity plant 37. Therefore, in the production of the lactic acid fermented bamboo powder 1 of the first and second embodiments, it is also possible to mix an acidic plant 37 such as Boysenberry instead of the plant enzyme 15. The plant enzyme 15 and the acidity plant 37 are preferably blended in the form of powder or granules, but may be blended in the form of strips or other states.

各実施形態では、一次乳酸発酵過程での酢酸エチルの生成を抑えるために、真空容器41を利用してその内部を真空に維持するようにしている。しかし、発酵容器内に窒素ガス等の不活性ガスを注入して不活性ガス雰囲気中で竹粉1を一次乳酸発酵させても、一次乳酸発酵過程での酢酸エチルの生成を抑えることが可能である。   In each embodiment, in order to suppress the production | generation of ethyl acetate in a primary lactic acid fermentation process, the inside is maintained to a vacuum using the vacuum vessel 41. FIG. However, even if an inert gas such as nitrogen gas is injected into the fermentation vessel and the bamboo powder 1 is subjected to primary lactic acid fermentation in an inert gas atmosphere, it is possible to suppress the production of ethyl acetate during the primary lactic acid fermentation process. is there.

第4、第5の実施形態の配合工程では、用途に応じて竹炭粉を配合してもよい。乳酸発酵竹粉1、乳酸発酵竹液22の用途によっては、微量の酢酸エチルの発生が全く問題にならない場合があるが、そのような用途に使用する乳酸発酵竹粉1、乳酸発酵竹液22の製造に際しては、通常の嫌気条件下で竹粉を乳酸発酵させるようにしてもよい。   In the blending steps of the fourth and fifth embodiments, bamboo charcoal powder may be blended depending on the application. Depending on the uses of the lactic acid fermented bamboo powder 1 and the lactic acid fermented bamboo liquid 22, the generation of a small amount of ethyl acetate may not be a problem at all. In the production of, bamboo powder may be lactic acid fermented under normal anaerobic conditions.

1 乳酸発酵竹粉
4 真空乳酸発酵工程
6 配合工程
7 二次発酵工程
11 竹粉
12 竹葉粉
13 竹根粉
14 乳酸発酵原料竹粉
15 植物酵素
16 竹炭粉
22 乳酸発酵竹液
23 竹液発酵工程
37 酸性質植物
1 Lactic acid fermentation bamboo powder 4 Vacuum lactic acid fermentation process 6 Compounding process 7 Secondary fermentation process 11 Bamboo powder 12 Bamboo leaf powder 13 Bamboo powder 14 Lactic acid fermentation raw material bamboo powder 15 Plant enzyme 16 Bamboo charcoal powder 22 Lactic acid fermentation bamboo liquid 23 Bamboo liquid fermentation Process 37 acid nature plant

本発明は、乳酸発酵竹液の製造方法に関するものである。 The present invention relates to a method for producing a lactic acid fermented bamboo liquid .

本発明は、このような従来の問題点に鑑み、嵩張りがなく、用途に応じて容易に活用でき、しかも有用性に優れた乳酸発酵竹液を製造する乳酸発酵竹液の製造方法を提供することを目的とするものである。 In view of such conventional problems, the present invention provides a method for producing a lactic acid fermented bamboo liquid that is not bulky, can be easily used according to the application, and produces a lactic acid fermented bamboo liquid excellent in utility. It is intended to do.

本発明は、竹粉を一次乳酸発酵させた乳酸発酵竹粉に、竹葉粉と、竹根粉と、pH7未満の酸性を有する粉状、顆粒状又は細片状の天然植物とを配合し、その配合粉を容器内の水中に浸漬し、前記容器内の空気を不活性ガスに置換して静置し、前記水中で前記配合粉を二次発酵させて二次発酵エキスを抽出するものである。 In the present invention , bamboo leaf powder, bamboo root powder , and powdery, granular, or flake shaped natural plants having an acidity of less than pH 7 are blended into lactic acid fermented bamboo powder obtained by subjecting bamboo powder to primary lactic acid fermentation. , Soaking the blended powder in water in the container, replacing the air in the container with an inert gas and allowing to stand, and subjecting the blended powder to secondary fermentation in the water to extract a secondary fermentation extract It is.

Claims (10)

乳酸発酵竹粉に添加物を配合し水中で二次発酵させて二次発酵エキスを抽出したことを特徴とする乳酸発酵竹液。   Lactic acid fermented bamboo liquid, characterized in that an additive is mixed with lactic acid fermented bamboo powder and subjected to secondary fermentation in water to extract a secondary fermented extract. 乳酸発酵竹粉に竹葉粉と竹根粉とpH7未満の酸性質植物とを配合し水中で発酵させてエキスを抽出したことを特徴とする乳酸発酵竹液。   A lactic acid fermented bamboo liquid, characterized in that a lactic acid fermented bamboo powder is blended with bamboo leaf powder, bamboo root powder, and an acidic plant having a pH of less than 7 and fermented in water to extract an extract. 前記竹葉粉は前記乳酸発酵竹粉の90〜110重量%であり、前記竹根粉は前記乳酸発酵竹粉の30〜50重量%であることを特徴とする請求項2に記載の乳酸発酵竹液。   The lactic acid fermentation according to claim 2, wherein the bamboo leaf powder is 90 to 110% by weight of the lactic acid fermented bamboo powder, and the bamboo root powder is 30 to 50% by weight of the lactic acid fermented bamboo powder. Bamboo liquid. 前記酸性質植物はベリー類、柑橘類等の果実であることを特徴とする請求項2又は3に記載の乳酸発酵竹液。   The lactic acid fermented bamboo solution according to claim 2 or 3, wherein the acidic plant is a fruit such as berries and citrus fruits. 前記果実はブルーベリー、ボイセンベリー等のベリー類であることを特徴とする請求項4に記載の乳酸発酵竹液。   The lactic acid fermented bamboo liquid according to claim 4, wherein the fruit is a berry such as a blueberry or a boysenberry. 前記ベリー類は前記乳酸発酵竹粉、前記竹葉粉及び前記竹根粉の総重量の3重量%以下であることを特徴とする請求項5に記載の乳酸発酵竹液。   6. The lactic acid fermented bamboo liquid according to claim 5, wherein the berries are 3% by weight or less of the total weight of the lactic acid fermented bamboo powder, the bamboo leaf powder and the bamboo root powder. 竹粉を一次乳酸発酵させた乳酸発酵竹粉に竹葉粉と竹根粉とpH7未満の酸性質植物とを配合し、この配合後の乳酸発酵竹粉を水中に浸漬して二次発酵させてエキスを抽出することを特徴とする乳酸発酵竹液の製造方法。   Bamboo leaf powder, bamboo root powder, and acidity plant having a pH of less than 7 are blended with lactic acid fermented bamboo powder obtained by primary lactic fermentation of bamboo powder. Extracting the extract, a method for producing a lactic acid fermented bamboo liquid. 前記一次乳酸発酵を真空下で行うことを特徴とする請求項7に記載の乳酸発酵竹液の製造方法。   The method for producing a lactic acid fermented bamboo liquid according to claim 7, wherein the primary lactic acid fermentation is performed under vacuum. 前記竹葉粉、前記竹根粉及び酸性質植物を配合した乳酸発酵竹粉を浸漬容器内の水中に浸漬した後、該浸漬容器内の空気を不活性ガスに置換して二次発酵を行うことを特徴とする請求項7又は8に記載の乳酸発酵竹液の製造方法。   After immersing the lactic acid fermented bamboo powder containing the bamboo leaf powder, the bamboo root powder and the acidity plant in water in an immersion container, the air in the immersion container is replaced with an inert gas to perform secondary fermentation. The method for producing a lactic acid fermented bamboo liquid according to claim 7 or 8. エキスの抽出後にオートクレーブ滅菌を行うことを特徴とする請求項7〜9の何れかに記載の乳酸発酵竹液の製造方法。   The method for producing a lactic acid fermented bamboo liquid according to any one of claims 7 to 9, wherein autoclave sterilization is performed after extraction of the extract.
JP2018078444A 2012-09-04 2018-04-16 Method for producing lactic acid fermented bamboo liquid Active JP6499348B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2012193850 2012-09-04
JP2012193850 2012-09-04

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2013182237A Division JP6371045B2 (en) 2012-09-04 2013-09-03 Method for producing lactic acid fermented bamboo liquid

Publications (2)

Publication Number Publication Date
JP2018143246A true JP2018143246A (en) 2018-09-20
JP6499348B2 JP6499348B2 (en) 2019-04-10

Family

ID=50741622

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2013182236A Pending JP2014064564A (en) 2012-09-04 2013-09-03 Method of manufacturing lactic fermented bamboo powder
JP2013182237A Active JP6371045B2 (en) 2012-09-04 2013-09-03 Method for producing lactic acid fermented bamboo liquid
JP2018078444A Active JP6499348B2 (en) 2012-09-04 2018-04-16 Method for producing lactic acid fermented bamboo liquid

Family Applications Before (2)

Application Number Title Priority Date Filing Date
JP2013182236A Pending JP2014064564A (en) 2012-09-04 2013-09-03 Method of manufacturing lactic fermented bamboo powder
JP2013182237A Active JP6371045B2 (en) 2012-09-04 2013-09-03 Method for producing lactic acid fermented bamboo liquid

Country Status (1)

Country Link
JP (3) JP2014064564A (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016044235A (en) * 2014-08-22 2016-04-04 希望の丘農園株式会社 Soil improving material and plant cultivation method
AU2016279383B9 (en) * 2015-06-15 2019-10-24 Sancho Co., Ltd. Method for producing fermented bamboo extract and immunostimulator
KR102252280B1 (en) * 2018-11-30 2021-05-14 (주)엔에프씨생명과학 Manufacturing method of fermented bamboo shoot with increased dietary fiber and antioxidant activity
KR102327236B1 (en) * 2019-08-05 2021-11-17 구스타 주식회사 Compositions for Anti-microbial and Deodorization Effect Comprising Complex Extract of Plants
TW202120460A (en) * 2019-11-05 2021-06-01 日商東京農大發股份有限公司全國土之會 Seedling culture soil/soil for agriculture and horticulture, and method for producing same
JP7085174B1 (en) * 2021-03-23 2022-06-16 長峰製茶株式会社 Manufacturing method of anaerobic fermented tea

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005058222A (en) * 2003-07-29 2005-03-10 Shinko Engineering Co Ltd Basic feed for livestock having bamboo as raw material, mixed feed, and method for producing the basic feed and the mixed feed
JP2007020561A (en) * 2005-06-16 2007-02-01 Shinko Koki Kk Method for producing bamboo powder pellet
JP2007161604A (en) * 2005-12-09 2007-06-28 Kitajima Shokuhin Kk Method for producing bamboo liquid and bamboo liquid
JP2008081604A (en) * 2006-09-27 2008-04-10 Micro Media Japan Corp Soil improvement method and improved soil
KR20090055525A (en) * 2009-04-03 2009-06-02 조지현 Feed additive containing an extract of bamboo and microorganism cultivate,for prevention of porcinediarrhea caused by enterotoxigenic enterobacteria
JP2010029110A (en) * 2008-07-29 2010-02-12 Anna Probio Kk Lactic fermented bamboo powder and lactic fermented bamboo liquid

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003259835A (en) * 2001-12-28 2003-09-16 Oubiken:Kk Production of fermented product and its utilization
JP2009189294A (en) * 2008-02-14 2009-08-27 Shikoku Kiki Kk Method for producing sealed lactic fermented bamboo powder product
JP4788978B2 (en) * 2008-09-29 2011-10-05 キャタピラー九州株式会社 Raw bamboo powder fermented feed and method for producing the same

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005058222A (en) * 2003-07-29 2005-03-10 Shinko Engineering Co Ltd Basic feed for livestock having bamboo as raw material, mixed feed, and method for producing the basic feed and the mixed feed
JP2007020561A (en) * 2005-06-16 2007-02-01 Shinko Koki Kk Method for producing bamboo powder pellet
JP2007161604A (en) * 2005-12-09 2007-06-28 Kitajima Shokuhin Kk Method for producing bamboo liquid and bamboo liquid
JP2008081604A (en) * 2006-09-27 2008-04-10 Micro Media Japan Corp Soil improvement method and improved soil
JP2010029110A (en) * 2008-07-29 2010-02-12 Anna Probio Kk Lactic fermented bamboo powder and lactic fermented bamboo liquid
KR20090055525A (en) * 2009-04-03 2009-06-02 조지현 Feed additive containing an extract of bamboo and microorganism cultivate,for prevention of porcinediarrhea caused by enterotoxigenic enterobacteria

Also Published As

Publication number Publication date
JP6371045B2 (en) 2018-08-08
JP2014064565A (en) 2014-04-17
JP6499348B2 (en) 2019-04-10
JP2014064564A (en) 2014-04-17

Similar Documents

Publication Publication Date Title
JP6499348B2 (en) Method for producing lactic acid fermented bamboo liquid
KR20170067927A (en) Livestock Nutrient Supplement and Preparing Method Thereof
CN107348099A (en) A kind of graminous pasture and the Silaging method of alfalfa
KR20080040296A (en) The method to develop fertilizer and feed for the consolidate fe(ferrum) agricultural produce by using and processing animal blood
Ashbell et al. Examination of a technology for silage making in plastic bags
Tagirova et al. Food and ecological safety of grape by-products
WO2015128892A1 (en) Lactic acid-fermented bamboo liquid, lactic acid-fermented bamboo agent, and method for manufacturing lactic acid-fermented bamboo liquid
CN107926195A (en) A kind of storage practice for improving wheat seed germinating rate
CN104855506B (en) A kind of mango storage fresh-keeping technique
Müller et al. Effect of anaerobic digestion residue vs. livestock manure and inorganic fertilizer on the hygienic quality of silage and haylage in bales
CN105532754B (en) A kind of infested natural plants combined extracts to go mouldy of prevention grain
CN107668427A (en) One kind is without sucrose corn purple sweet potato juice and preparation method thereof
RU2400964C2 (en) Method of carrots storage
CN109619172A (en) Organic citrus preservation method
Temur et al. Some aspects of recycling and storage of secondary resources of grape
WO2023120663A1 (en) Ruminant feed composition, ruminant feed, and method for manufacturing ruminant feed composition
KR101880672B1 (en) A method for preparing washing solution comprising flaxseed pomace extract for washing fresh-cut vegetables and a microbial decontamination method for fresh-cut vegetables using the same
EP0075614A1 (en) Method for the selective production of mycelia and fruit of basidiomycetes, application of the mycelia and apparatus for carrying out the method
JP2015168622A (en) Anti-microbe agent, flavor promoter, and growth promoter
CN106117867A (en) A kind of bacteriostasis, preservation packaging material for food and preparation method thereof
Lifa et al. Chemical and fermentative characteristics of agricultural byproducts and their mixtures with roughages incubated with rumen fluid from slaughtered dromedaries
CN103444860B (en) Preparation method of tea polysaccharide composite film biological preservative and application of biological preservative in fruits and vegetables
CN107459408A (en) A kind of wine-growing bio-feritlizer and preparation method
Handoko et al. Journal of Applied Food Technology
EP1371297A1 (en) Method for producing a health food additive and said health food additive

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180423

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180423

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20190124

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190219

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190314

R150 Certificate of patent or registration of utility model

Ref document number: 6499348

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250