JP2018139614A - 細胞観察装置及び細胞観察方法 - Google Patents
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
- G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
- G01N15/1431—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing the electronics being integrated with the analyser, e.g. hand-held devices for on-site investigation
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G01N15/10—Investigating individual particles
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- G—PHYSICS
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- G01N15/10—Investigating individual particles
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
Description
Claims (8)
- 細胞を含む試料を保持するウェルが複数配列されたマイクロプレートによって保持された前記細胞から放出された光を測定する細胞観察装置であって、
前記マイクロプレートを載置するための載置部と、
陽極と陰極とを含む電極対が複数配列された電気刺激部と、
前記マイクロプレートの前記ウェル内に前記電極対が配置されるように、前記電気刺激部の位置を制御する位置制御部と、
前記マイクロプレートの前記ウェル内の前記試料からの光を検出する光検出部と、
前記光検出部による検出結果に応じて得られた光強度分布を対象に、前記ウェルの境界の位置を特定し、前記ウェルの境界の位置を基に解析領域を設定し、前記解析領域の光強度を解析することによって、前記試料に関する解析情報を取得する情報解析部と、
を備える細胞観察装置。 - 前記情報解析部は、前記光強度分布における輝度差に基づいて、前記ウェルの境界の位置を特定する、
請求項1記載の細胞観察装置。 - 前記情報解析部は、前記電極対に印加される電位に応じて、前記解析領域の大きさを設定する、請求項1又は2に記載の細胞観察装置。
- 前記位置制御部は、筋細胞を含む前記試料を保持する前記ウェル内に前記電極対が配置されるように制御し、
前記情報解析部は、前記電極対に繰り返しパルス電圧を印加した状態での前記解析領域の光強度を解析する、
請求項1〜3のいずれか1項に記載の細胞観察装置。 - 細胞を含む試料を保持するウェルが複数配列されたマイクロプレートによって保持された前記細胞から放出された光を測定する細胞観察方法であって、
前記マイクロプレートを載置部に載置する載置ステップと、
陽極と陰極とを含む電極対が複数配列された電気刺激部に対して、前記マイクロプレートの前記ウェル内に前記電極対が配置されるように、位置を制御する位置制御ステップと、
前記マイクロプレートの前記ウェル内の前記試料からの光を検出する光検出ステップと、
前記光検出ステップにおける検出結果に応じて得られた光強度分布を対象に、前記ウェルの境界の位置を特定し、前記ウェルの境界の位置を基に解析領域に設定し、前記解析領域の光強度を解析することによって、前記試料に関する解析情報を取得する情報解析ステップと、
を備える細胞観察方法。 - 前記情報解析ステップでは、前記光強度分布における輝度差に基づいて、前記ウェルの境界の位置を特定する、
請求項5に記載の細胞観察方法。 - 前記情報解析ステップでは、前記電極対に印加される電位に応じて、前記解析領域の大きさを設定する、
請求項5又は6に記載の細胞観察方法。 - 前記位置制御ステップでは、筋細胞を含む前記試料を保持する前記ウェル内に前記電極対が配置されるように制御し、
前記情報解析ステップでは、前記電極対に繰り返しパルス電圧を印加した状態での前記解析領域の光強度を解析する、
請求項5〜7のいずれか1項に記載の細胞観察方法。
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AU2015300778B2 (en) | 2014-08-08 | 2021-02-25 | Quantum-Si Incorporated | Optical system and assay chip for probing, detecting, and analyzing molecules |
US10677709B2 (en) * | 2017-04-28 | 2020-06-09 | Becton, Dickinson And Company | Particle detection cartridges, systems thereof and methods for using the same |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005514909A (ja) * | 2001-07-12 | 2005-05-26 | メルク エンド カムパニー インコーポレーテッド | 真核生物細胞の電場による刺激 |
WO2011132586A1 (ja) * | 2010-04-23 | 2011-10-27 | 浜松ホトニクス株式会社 | 細胞観察装置および細胞観察方法 |
JP2012501646A (ja) * | 2008-09-04 | 2012-01-26 | ガレニア コーポレーション | シナプス小胞サイクリングアッセイおよびシステム |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0581983B1 (en) * | 1992-08-04 | 1996-10-23 | Hewlett-Packard GmbH | Apparatus for performing capillary electrophoresis |
AU729397B2 (en) * | 1997-05-01 | 2001-02-01 | Sophion Bioscience A/S | An automatic electrode positioning apparatus |
JP4327970B2 (ja) * | 1999-12-28 | 2009-09-09 | 浜松ホトニクス株式会社 | 発光測定装置及び蛍光測定装置 |
US7615356B2 (en) | 2000-07-10 | 2009-11-10 | Vertex Pharmaceuticals (San Diego) Llc | Ion channel assay methods |
MXJL03000001A (es) * | 2000-07-10 | 2004-02-09 | Vertex Pharma San Diego Llc | Metodos de ensayo de canales de iones. |
US6783649B2 (en) * | 2000-12-01 | 2004-08-31 | Cetek Corporation | High throughput capillary electrophoresis system |
US20060199168A1 (en) * | 2002-05-31 | 2006-09-07 | Wills Ivan N | Cell recovery |
JP2004147517A (ja) * | 2002-10-29 | 2004-05-27 | Hitachi Ltd | 遺伝子導入装置 |
US8658349B2 (en) * | 2006-07-13 | 2014-02-25 | Seahorse Bioscience | Cell analysis apparatus and method |
JP2007135947A (ja) * | 2005-11-21 | 2007-06-07 | Fujifilm Corp | 光プローブおよび光断層画像化装置 |
WO2009130814A1 (ja) * | 2008-04-21 | 2009-10-29 | 国立大学法人名古屋大学 | 細胞組織磁気信号検出装置 |
JP5219898B2 (ja) | 2009-03-26 | 2013-06-26 | 浜松ホトニクス株式会社 | 光照射装置及び光測定装置 |
JP5219899B2 (ja) | 2009-03-26 | 2013-06-26 | 浜松ホトニクス株式会社 | 光照射装置及び光測定装置 |
CN102449163A (zh) * | 2009-04-03 | 2012-05-09 | 加利福尼亚大学董事会 | 分选细胞和其它生物微粒的方法和装置 |
WO2011063086A1 (en) * | 2009-11-19 | 2011-05-26 | Halliburton Energy Services, Inc. | Downhole optical radiometry tool |
WO2012066747A1 (ja) * | 2010-11-17 | 2012-05-24 | パナソニック株式会社 | 微生物数測定装置 |
US11169086B2 (en) * | 2015-04-10 | 2021-11-09 | Blaze Metrics, LLC | System and method for simultaneously performing multiple optical analyses of liquids and particles in a fluid |
-
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005514909A (ja) * | 2001-07-12 | 2005-05-26 | メルク エンド カムパニー インコーポレーテッド | 真核生物細胞の電場による刺激 |
JP2012501646A (ja) * | 2008-09-04 | 2012-01-26 | ガレニア コーポレーション | シナプス小胞サイクリングアッセイおよびシステム |
WO2011132586A1 (ja) * | 2010-04-23 | 2011-10-27 | 浜松ホトニクス株式会社 | 細胞観察装置および細胞観察方法 |
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