JP2017538115A5 - - Google Patents
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- Publication number
- JP2017538115A5 JP2017538115A5 JP2017527737A JP2017527737A JP2017538115A5 JP 2017538115 A5 JP2017538115 A5 JP 2017538115A5 JP 2017527737 A JP2017527737 A JP 2017527737A JP 2017527737 A JP2017527737 A JP 2017527737A JP 2017538115 A5 JP2017538115 A5 JP 2017538115A5
- Authority
- JP
- Japan
- Prior art keywords
- cartridge
- hybridized
- sample
- affinity matrix
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 48
- 239000011324 bead Substances 0.000 claims 23
- 239000000872 buffer Substances 0.000 claims 21
- 239000011159 matrix material Substances 0.000 claims 21
- 238000000034 method Methods 0.000 claims 15
- 108091034117 Oligonucleotide Proteins 0.000 claims 10
- 238000010828 elution Methods 0.000 claims 8
- 238000003384 imaging method Methods 0.000 claims 8
- 239000012530 fluid Substances 0.000 claims 7
- 239000002699 waste material Substances 0.000 claims 4
- 108090001008 Avidin Proteins 0.000 claims 2
- 108010090804 Streptavidin Proteins 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 238000005562 fading Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462083681P | 2014-11-24 | 2014-11-24 | |
| US62/083,681 | 2014-11-24 | ||
| PCT/US2015/062109 WO2016085841A1 (en) | 2014-11-24 | 2015-11-23 | Methods and apparatuses for gene purification and imaging |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020118680A Division JP2020191881A (ja) | 2014-11-24 | 2020-07-09 | 遺伝子精製および画像化のための方法および装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017538115A JP2017538115A (ja) | 2017-12-21 |
| JP2017538115A5 true JP2017538115A5 (https=) | 2019-10-10 |
| JP7144142B2 JP7144142B2 (ja) | 2022-09-29 |
Family
ID=55022671
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017527737A Active JP7144142B2 (ja) | 2014-11-24 | 2015-11-23 | 遺伝子精製および画像化のための方法および装置 |
| JP2020118680A Pending JP2020191881A (ja) | 2014-11-24 | 2020-07-09 | 遺伝子精製および画像化のための方法および装置 |
| JP2022125527A Pending JP2022140792A (ja) | 2014-11-24 | 2022-08-05 | 遺伝子精製および画像化のための方法および装置 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020118680A Pending JP2020191881A (ja) | 2014-11-24 | 2020-07-09 | 遺伝子精製および画像化のための方法および装置 |
| JP2022125527A Pending JP2022140792A (ja) | 2014-11-24 | 2022-08-05 | 遺伝子精製および画像化のための方法および装置 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US10246700B2 (https=) |
| EP (1) | EP3223947B1 (https=) |
| JP (3) | JP7144142B2 (https=) |
| KR (1) | KR102602507B1 (https=) |
| CN (1) | CN107249741B (https=) |
| AU (1) | AU2015353747B2 (https=) |
| CA (1) | CA2968519C (https=) |
| ES (1) | ES2756399T3 (https=) |
| SG (1) | SG11201704177SA (https=) |
| WO (1) | WO2016085841A1 (https=) |
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| EP3604555B1 (en) | 2011-10-14 | 2024-12-25 | President and Fellows of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP2971184B1 (en) | 2013-03-12 | 2019-04-17 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
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| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| KR102602507B1 (ko) | 2014-11-24 | 2023-11-14 | 나노스트링 테크놀로지스, 인크. | 유전자 정제 및 이미징을 위한 방법 및 장치 |
| EP3901282B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| EP4613756A3 (en) | 2016-04-25 | 2025-11-12 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| US11352667B2 (en) | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
| CN118853848A (zh) | 2016-08-31 | 2024-10-29 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
| CN120210336A (zh) | 2017-10-06 | 2025-06-27 | 10X基因组学有限公司 | Rna模板化连接 |
| EP3731959B1 (en) | 2017-12-29 | 2025-10-08 | Clear Labs, Inc. | Automated priming and library loading device |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN119220641A (zh) | 2019-05-31 | 2024-12-31 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| EP4097251A1 (en) | 2020-01-29 | 2022-12-07 | 10X Genomics, Inc. | Compositions and methods for analyte detection |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| CN115485391A (zh) | 2020-02-17 | 2022-12-16 | 10X基因组学有限公司 | 染色质相互作用的原位分析 |
| EP4484571A3 (en) | 2020-02-21 | 2025-03-19 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| GB202003827D0 (en) * | 2020-03-17 | 2020-04-29 | Applied Biotect Ltd | Mircofludic device and method |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4414459B1 (en) | 2020-05-22 | 2025-09-03 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| WO2022061150A2 (en) | 2020-09-18 | 2022-03-24 | 10X Geonomics, Inc. | Sample handling apparatus and image registration methods |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US12509717B2 (en) | 2020-12-11 | 2025-12-30 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| EP4421491A3 (en) | 2021-02-19 | 2024-11-27 | 10X Genomics, Inc. | Method of using a modular assay support device |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| AU2022317053B2 (en) | 2021-07-30 | 2025-01-02 | 10X Genomics, Inc. | Methods and compositions for synchronizing reactions in situ |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| US12529096B2 (en) | 2021-08-03 | 2026-01-20 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid structures |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| US12553079B2 (en) | 2021-08-03 | 2026-02-17 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
| EP4446426B1 (en) | 2021-08-16 | 2026-03-04 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| USD1064308S1 (en) | 2021-09-17 | 2025-02-25 | 10X Genomics, Inc. | Sample handling device |
| WO2023122033A1 (en) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| EP4733410A2 (en) | 2022-01-21 | 2026-04-29 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
| CN118974836A (zh) | 2022-03-08 | 2024-11-15 | 10X基因组学有限公司 | 将光学拥挤最小化的原位代码设计方法 |
| WO2023183881A2 (en) | 2022-03-24 | 2023-09-28 | Digital Biology Inc. | Tissue spatial omics |
| US20250271415A1 (en) * | 2022-03-29 | 2025-08-28 | Sony Group Corporation | Bioparticle analysis system, information processing device, and bioparticle analysis method |
| EP4505177A1 (en) | 2022-04-01 | 2025-02-12 | 10x Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| WO2023215603A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| US20240084378A1 (en) | 2022-05-11 | 2024-03-14 | 10X Genomics, Inc. | Compositions and methods for in situ sequencing |
| EP4540607A1 (en) | 2022-06-17 | 2025-04-23 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| AU2023308992A1 (en) | 2022-07-19 | 2025-01-30 | Digital Biology Inc. | Barcode diffusion-based spatial omics |
| WO2024040060A1 (en) | 2022-08-16 | 2024-02-22 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| EP1585957A2 (en) | 2001-11-27 | 2005-10-19 | Burstein Technologies, Inc. | Magneto-optical bio-discs and systems including related methods |
| US7217542B2 (en) * | 2002-10-31 | 2007-05-15 | Hewlett-Packard Development Company, L.P. | Microfluidic system for analyzing nucleic acids |
| EP1954708A4 (en) | 2005-11-23 | 2009-05-13 | Univ Utah Res Found | Methods and compositions involving intrinsic genes |
| JP5700911B2 (ja) | 2005-12-23 | 2015-04-15 | ナノストリング テクノロジーズ，インコーポレーテッド | 配向され、固定化された巨大分子を含む組成物とその製造法 |
| WO2007076128A2 (en) | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| ES2393758T3 (es) | 2006-03-15 | 2012-12-27 | Micronics, Inc. | Ensayos integrados de ácidos nucleicos |
| CA2653095C (en) | 2006-05-22 | 2013-07-16 | Nanostring Technologies, Inc. | Systems and methods for analyzing nanoreporters |
| CA2687292C (en) | 2007-04-10 | 2017-07-04 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| EP1990638A1 (en) | 2007-05-11 | 2008-11-12 | Koninklijke Philips Electronics N.V. | Flow-through biosensor |
| ES2457534T3 (es) | 2008-05-30 | 2014-04-28 | The University Of North Carolina At Chapel Hill | Perfiles de expresión génica para predecir desenlaces en cáncer de mama |
| US8519115B2 (en) | 2008-08-14 | 2013-08-27 | Nanostring Technologies, Inc. | Stable nanoreporters |
| WO2011047087A2 (en) | 2009-10-13 | 2011-04-21 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
| EP2516681B1 (en) | 2010-02-11 | 2017-10-18 | Nanostring Technologies, Inc | Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation |
| US20110229888A1 (en) | 2010-03-16 | 2011-09-22 | Hengen Paul N | Compositions and Methods for the Detection of Genomic Features |
| EP2685988A4 (en) | 2011-03-15 | 2014-08-20 | Univ North Carolina | Methods of treating breast cancer with anthracycline therapy |
| US9758834B2 (en) | 2011-03-28 | 2017-09-12 | Nanostring Technologies, Inc. | Compositions and methods for diagnosing cancer |
| CA2839705A1 (en) * | 2011-06-24 | 2012-12-27 | Nanostring Technologies, Inc. | Multivariate diagnostic assays and methods for using same |
| JP6144695B2 (ja) * | 2011-11-30 | 2017-06-07 | ユニバーシティー オブ ノースカロライナ アット チャペル ヒル | タキサン療法を用いて乳癌を処置する方法 |
| CA2874492C (en) * | 2012-05-22 | 2021-10-19 | British Columbia Cancer Agency Branch | Nano46 genes and methods to predict breast cancer outcome |
| US20140037620A1 (en) | 2012-06-29 | 2014-02-06 | British Columbia Cancer Agency Branch | Methods of Treating Breast Cancer with Gemcitabine Therapy |
| US20140200167A1 (en) * | 2012-08-01 | 2014-07-17 | Nanomdx, Inc. | Functionally integrated device for multiplex genetic identification |
| WO2014075067A1 (en) | 2012-11-12 | 2014-05-15 | Nanostring Technologies, Inc. | Methods to predict breast cancer outcome |
| AU2014278152A1 (en) | 2013-06-14 | 2015-12-24 | Nanostring Technologies, Inc. | Multiplexable tag-based reporter system |
| CA2923166A1 (en) | 2013-09-09 | 2015-03-12 | British Columbia Cancer Agency Branch | Methods and kits for predicting outcome and methods and kits for treating breast cancer with radiation therapy |
| KR102602507B1 (ko) | 2014-11-24 | 2023-11-14 | 나노스트링 테크놀로지스, 인크. | 유전자 정제 및 이미징을 위한 방법 및 장치 |
-
2015
- 2015-11-23 KR KR1020177017400A patent/KR102602507B1/ko active Active
- 2015-11-23 EP EP15816281.8A patent/EP3223947B1/en active Active
- 2015-11-23 US US14/948,776 patent/US10246700B2/en active Active
- 2015-11-23 WO PCT/US2015/062109 patent/WO2016085841A1/en not_active Ceased
- 2015-11-23 CA CA2968519A patent/CA2968519C/en active Active
- 2015-11-23 ES ES15816281T patent/ES2756399T3/es active Active
- 2015-11-23 CN CN201580074230.5A patent/CN107249741B/zh active Active
- 2015-11-23 JP JP2017527737A patent/JP7144142B2/ja active Active
- 2015-11-23 SG SG11201704177SA patent/SG11201704177SA/en unknown
- 2015-11-23 AU AU2015353747A patent/AU2015353747B2/en not_active Ceased
-
2019
- 2019-02-11 US US16/272,073 patent/US11098301B2/en not_active Expired - Fee Related
-
2020
- 2020-07-09 JP JP2020118680A patent/JP2020191881A/ja active Pending
-
2022
- 2022-08-05 JP JP2022125527A patent/JP2022140792A/ja active Pending
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