JP2017538115A5 - - Google Patents
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- JP2017538115A5 JP2017538115A5 JP2017527737A JP2017527737A JP2017538115A5 JP 2017538115 A5 JP2017538115 A5 JP 2017538115A5 JP 2017527737 A JP2017527737 A JP 2017527737A JP 2017527737 A JP2017527737 A JP 2017527737A JP 2017538115 A5 JP2017538115 A5 JP 2017538115A5
- Authority
- JP
- Japan
- Prior art keywords
- cartridge
- hybridized
- sample
- affinity matrix
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 48
- 239000011324 bead Substances 0.000 claims 23
- 239000000872 buffer Substances 0.000 claims 21
- 239000011159 matrix material Substances 0.000 claims 21
- 238000000034 method Methods 0.000 claims 15
- 108091034117 Oligonucleotide Proteins 0.000 claims 10
- 238000010828 elution Methods 0.000 claims 8
- 238000003384 imaging method Methods 0.000 claims 8
- 239000012530 fluid Substances 0.000 claims 7
- 239000002699 waste material Substances 0.000 claims 4
- 108090001008 Avidin Proteins 0.000 claims 2
- 108010090804 Streptavidin Proteins 0.000 claims 2
- 230000001419 dependent effect Effects 0.000 claims 2
- 238000010438 heat treatment Methods 0.000 claims 2
- 238000005562 fading Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462083681P | 2014-11-24 | 2014-11-24 | |
| US62/083,681 | 2014-11-24 | ||
| PCT/US2015/062109 WO2016085841A1 (en) | 2014-11-24 | 2015-11-23 | Methods and apparatuses for gene purification and imaging |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020118680A Division JP2020191881A (ja) | 2014-11-24 | 2020-07-09 | 遺伝子精製および画像化のための方法および装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017538115A JP2017538115A (ja) | 2017-12-21 |
| JP2017538115A5 true JP2017538115A5 (https=) | 2019-10-10 |
| JP7144142B2 JP7144142B2 (ja) | 2022-09-29 |
Family
ID=55022671
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017527737A Active JP7144142B2 (ja) | 2014-11-24 | 2015-11-23 | 遺伝子精製および画像化のための方法および装置 |
| JP2020118680A Pending JP2020191881A (ja) | 2014-11-24 | 2020-07-09 | 遺伝子精製および画像化のための方法および装置 |
| JP2022125527A Pending JP2022140792A (ja) | 2014-11-24 | 2022-08-05 | 遺伝子精製および画像化のための方法および装置 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020118680A Pending JP2020191881A (ja) | 2014-11-24 | 2020-07-09 | 遺伝子精製および画像化のための方法および装置 |
| JP2022125527A Pending JP2022140792A (ja) | 2014-11-24 | 2022-08-05 | 遺伝子精製および画像化のための方法および装置 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US10246700B2 (https=) |
| EP (1) | EP3223947B1 (https=) |
| JP (3) | JP7144142B2 (https=) |
| KR (1) | KR102602507B1 (https=) |
| CN (1) | CN107249741B (https=) |
| AU (1) | AU2015353747B2 (https=) |
| CA (1) | CA2968519C (https=) |
| ES (1) | ES2756399T3 (https=) |
| SG (1) | SG11201704177SA (https=) |
| WO (1) | WO2016085841A1 (https=) |
Families Citing this family (57)
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|---|---|---|---|---|
| WO2013055995A2 (en) | 2011-10-14 | 2013-04-18 | President And Fellows Of Harvard College | Sequencing by structure assembly |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| EP3578666A1 (en) | 2013-03-12 | 2019-12-11 | President and Fellows of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
| EP3013983B1 (en) | 2013-06-25 | 2023-02-15 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| JP7144142B2 (ja) | 2014-11-24 | 2022-09-29 | ナノストリング テクノロジーズ，インコーポレイティド | 遺伝子精製および画像化のための方法および装置 |
| US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| CN109415761B (zh) | 2016-04-25 | 2022-09-20 | 哈佛学院董事及会员团体 | 用于原位分子检测的杂交链反应方法 |
| EP4050112A1 (en) | 2016-06-21 | 2022-08-31 | 10X Genomics, Inc. | Nucleic acid sequencing |
| CN109923216B (zh) | 2016-08-31 | 2024-08-02 | 哈佛学院董事及会员团体 | 将生物分子的检测组合到使用荧光原位测序的单个试验的方法 |
| CA3078158A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | Rna templated ligation |
| AU2018353924A1 (en) | 2017-12-29 | 2019-07-18 | Clear Labs, Inc. | Automated priming and library loading device |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| WO2020240025A1 (en) | 2019-05-31 | 2020-12-03 | Cartana Ab | Method of detecting target nucleic acid molecules |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| WO2021155063A1 (en) | 2020-01-29 | 2021-08-05 | Readcoor, Llc | Compositions and methods for analyte detection |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| EP4107284A1 (en) | 2020-02-17 | 2022-12-28 | 10X Genomics, Inc. | In situ analysis of chromatin interaction |
| EP4484571A3 (en) | 2020-02-21 | 2025-03-19 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| GB202003827D0 (en) * | 2020-03-17 | 2020-04-29 | Applied Biotect Ltd | Mircofludic device and method |
| CN116134308B (zh) | 2020-05-19 | 2025-05-27 | 10X基因组学有限公司 | 电泳盒和仪器 |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| WO2021252747A1 (en) | 2020-06-10 | 2021-12-16 | 1Ox Genomics, Inc. | Fluid delivery methods |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| EP4491742B1 (en) | 2020-09-18 | 2026-03-04 | 10X Genomics, Inc. | Sample handling apparatus and image registration methods |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| EP4012046A1 (en) | 2020-12-11 | 2022-06-15 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| WO2022178267A2 (en) | 2021-02-19 | 2022-08-25 | 10X Genomics, Inc. | Modular assay support devices |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| CA3224093A1 (en) | 2021-07-30 | 2023-02-02 | Jorge Ivan Hernandez Neuta | Methods and compositions for synchronizing reactions in situ |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| US12529096B2 (en) | 2021-08-03 | 2026-01-20 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid structures |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| EP4381095A1 (en) | 2021-08-03 | 2024-06-12 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
| US12435364B2 (en) | 2021-08-16 | 2025-10-07 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| USD1064308S1 (en) | 2021-09-17 | 2025-02-25 | 10X Genomics, Inc. | Sample handling device |
| EP4441711A1 (en) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| EP4733410A2 (en) | 2022-01-21 | 2026-04-29 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
| EP4490737A1 (en) | 2022-03-08 | 2025-01-15 | 10X Genomics, Inc. | In situ code design methods for minimizing optical crowding |
| WO2023183881A2 (en) | 2022-03-24 | 2023-09-28 | Digital Biology Inc. | Tissue spatial omics |
| WO2023188896A1 (ja) * | 2022-03-29 | 2023-10-05 | ソニーグループ株式会社 | 生体粒子解析システム、情報処理装置、及び生体粒子解析方法 |
| AU2023243205A1 (en) | 2022-04-01 | 2024-09-19 | 10X Genomics, Inc. | Compositions and methods for targeted masking of autofluorescence |
| EP4519453A1 (en) | 2022-05-06 | 2025-03-12 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| CN119213143A (zh) | 2022-05-11 | 2024-12-27 | 10X基因组学有限公司 | 用于原位测序的组合物和方法 |
| WO2023245190A1 (en) | 2022-06-17 | 2023-12-21 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| EP4558647A2 (en) | 2022-07-19 | 2025-05-28 | Digital Biology Inc. | Barcode diffusion-based spatial omics |
| WO2024040060A1 (en) | 2022-08-16 | 2024-02-22 | 10X Genomics, Inc. | Ap50 polymerases and uses thereof |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| JP2005530127A (ja) * | 2001-11-27 | 2005-10-06 | バースタイン テクノロジーズ，インコーポレイティド | 光磁気バイオディスクおよび関連の方法を含むシステム |
| US7217542B2 (en) | 2002-10-31 | 2007-05-15 | Hewlett-Packard Development Company, L.P. | Microfluidic system for analyzing nucleic acids |
| CA2630974A1 (en) | 2005-11-23 | 2007-05-31 | University Of Utah Research Foundation | Methods and compositions involving intrinsic genes |
| US8986926B2 (en) | 2005-12-23 | 2015-03-24 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
| EP1963531B1 (en) | 2005-12-23 | 2011-09-21 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| JP5254949B2 (ja) * | 2006-03-15 | 2013-08-07 | マイクロニクス， インコーポレイテッド | 一体型の核酸アッセイ |
| JP5081232B2 (ja) | 2006-05-22 | 2012-11-28 | ナノストリング テクノロジーズ， インコーポレイテッド | ナノレポーターを分析するためのシステムおよび方法 |
| US8415102B2 (en) | 2007-04-10 | 2013-04-09 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| EP1990638A1 (en) * | 2007-05-11 | 2008-11-12 | Koninklijke Philips Electronics N.V. | Flow-through biosensor |
| DK2297359T3 (en) | 2008-05-30 | 2014-02-24 | Univ Utah Res Found | Gene expression profiles to predict the outcome of breast cancer |
| US8519115B2 (en) | 2008-08-14 | 2013-08-27 | Nanostring Technologies, Inc. | Stable nanoreporters |
| WO2011047087A2 (en) | 2009-10-13 | 2011-04-21 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
| EP2516681B1 (en) | 2010-02-11 | 2017-10-18 | Nanostring Technologies, Inc | Compositions and methods for the detection of preferably small rnas by bridge hybridisation and ligation |
| US20110229888A1 (en) | 2010-03-16 | 2011-09-22 | Hengen Paul N | Compositions and Methods for the Detection of Genomic Features |
| AU2012229123B2 (en) | 2011-03-15 | 2017-02-02 | British Columbia Cancer Agency Branch | Methods of treating breast cancer with anthracycline therapy |
| US9758834B2 (en) | 2011-03-28 | 2017-09-12 | Nanostring Technologies, Inc. | Compositions and methods for diagnosing cancer |
| US20130017971A1 (en) | 2011-06-24 | 2013-01-17 | Nanostring Technologies, Inc. | Multivariate Diagnostic Assays and Methods for Using Same |
| AU2012345789B2 (en) * | 2011-11-30 | 2018-02-15 | British Columbia Cancer Agency Branch | Methods of treating breast cancer with taxane therapy |
| AU2013266419B2 (en) * | 2012-05-22 | 2018-09-27 | British Columbia Cancer Agency Branch | NANO46 genes and methods to predict breast cancer outcome |
| WO2014005010A2 (en) | 2012-06-29 | 2014-01-03 | Nanostring Technologies, Inc. | Methods of treating breast cancer with gemcitabine therapy |
| US20140200167A1 (en) | 2012-08-01 | 2014-07-17 | Nanomdx, Inc. | Functionally integrated device for multiplex genetic identification |
| US20140154681A1 (en) | 2012-11-12 | 2014-06-05 | Nanostring Technologies, Inc. | Methods to Predict Breast Cancer Outcome |
| AU2014278152A1 (en) | 2013-06-14 | 2015-12-24 | Nanostring Technologies, Inc. | Multiplexable tag-based reporter system |
| EP3044332A1 (en) | 2013-09-09 | 2016-07-20 | British Columbia Cancer Agency Branch | Methods and kits for predicting outcome and methods and kits for treating breast cancer with radiation therapy |
| JP7144142B2 (ja) | 2014-11-24 | 2022-09-29 | ナノストリング テクノロジーズ，インコーポレイティド | 遺伝子精製および画像化のための方法および装置 |
-
2015
- 2015-11-23 JP JP2017527737A patent/JP7144142B2/ja active Active
- 2015-11-23 CA CA2968519A patent/CA2968519C/en active Active
- 2015-11-23 ES ES15816281T patent/ES2756399T3/es active Active
- 2015-11-23 EP EP15816281.8A patent/EP3223947B1/en active Active
- 2015-11-23 AU AU2015353747A patent/AU2015353747B2/en not_active Ceased
- 2015-11-23 WO PCT/US2015/062109 patent/WO2016085841A1/en not_active Ceased
- 2015-11-23 CN CN201580074230.5A patent/CN107249741B/zh active Active
- 2015-11-23 US US14/948,776 patent/US10246700B2/en active Active
- 2015-11-23 SG SG11201704177SA patent/SG11201704177SA/en unknown
- 2015-11-23 KR KR1020177017400A patent/KR102602507B1/ko active Active
-
2019
- 2019-02-11 US US16/272,073 patent/US11098301B2/en not_active Expired - Fee Related
-
2020
- 2020-07-09 JP JP2020118680A patent/JP2020191881A/ja active Pending
-
2022
- 2022-08-05 JP JP2022125527A patent/JP2022140792A/ja active Pending
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