JP2017529069A5 - - Google Patents
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- JP2017529069A5 JP2017529069A5 JP2017508557A JP2017508557A JP2017529069A5 JP 2017529069 A5 JP2017529069 A5 JP 2017529069A5 JP 2017508557 A JP2017508557 A JP 2017508557A JP 2017508557 A JP2017508557 A JP 2017508557A JP 2017529069 A5 JP2017529069 A5 JP 2017529069A5
- Authority
- JP
- Japan
- Prior art keywords
- hybridization
- solid support
- channel
- nucleic acid
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000009396 hybridization Methods 0.000 claims description 74
- 239000007787 solid Substances 0.000 claims description 44
- 239000000523 sample Substances 0.000 claims description 42
- 150000007523 nucleic acids Chemical class 0.000 claims description 41
- 238000000034 method Methods 0.000 claims description 33
- 239000011325 microbead Substances 0.000 claims description 33
- 108020004707 nucleic acids Proteins 0.000 claims description 31
- 102000039446 nucleic acids Human genes 0.000 claims description 31
- 230000000295 complement effect Effects 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 108091034117 Oligonucleotide Proteins 0.000 claims description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- 238000003825 pressing Methods 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000035475 disorder Diseases 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000012165 high-throughput sequencing Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1414451.3A GB201414451D0 (en) | 2014-08-14 | 2014-08-14 | Hybridisation column for nucleic acid enrichment |
| GB1414451.3 | 2014-08-14 | ||
| PCT/GB2015/052370 WO2016024134A2 (en) | 2014-08-14 | 2015-08-14 | Hybridisation column for nucleic acid enrichment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017529069A JP2017529069A (ja) | 2017-10-05 |
| JP2017529069A5 true JP2017529069A5 (https=) | 2018-09-20 |
Family
ID=51662437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017508557A Pending JP2017529069A (ja) | 2014-08-14 | 2015-08-14 | 核酸濃縮用のハイブリダイゼーションカラム |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20170226501A1 (https=) |
| EP (1) | EP3180444A2 (https=) |
| JP (1) | JP2017529069A (https=) |
| GB (1) | GB201414451D0 (https=) |
| WO (1) | WO2016024134A2 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101799192B1 (ko) * | 2016-03-24 | 2017-11-17 | 고려대학교 산학협력단 | 표적 유전자 검출을 위한 미세 유동 장치 |
| ES2920280T3 (es) | 2017-07-07 | 2022-08-02 | Nipd Genetics Public Company Ltd | Enriquecimiento de regiones genómicas blanco para análisis paralelo multiplexado |
| ES2925394T3 (es) | 2017-07-07 | 2022-10-17 | Nipd Genetics Public Company Ltd | Análisis paralelo multiplexado con enriquecimiento de blancos para la evaluación del riesgo de portar alteraciones genéticas |
| PT3649258T (pt) | 2017-07-07 | 2022-05-25 | Nipd Genetics Public Company Ltd | Análise paralela multiplexada enriquecida com alvo para avaliação de amostras de adn fetal |
| WO2019008172A1 (en) | 2017-07-07 | 2019-01-10 | Nipd Genetics Public Company Limited | ENRICHED MULTIPLEXED PARALLEL ANALYSIS IN TARGET FOR TUMOR BIOMARKER EVALUATION |
| CN118139987A (zh) * | 2021-09-23 | 2024-06-04 | 基因组检测合作社公司 | 用于cfrna和cftna靶向ngs测序的组合物和方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE214633T1 (de) * | 1993-10-28 | 2002-04-15 | Houston Advanced Res Ct | Mikrofabriziertes poröses durchflussgerät |
| US5763594A (en) * | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
| US6969488B2 (en) * | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US20030164335A1 (en) * | 1998-10-23 | 2003-09-04 | Grate Jay W. | Method for high throughput separations in microfluidic systems using small particles |
| ATE556149T1 (de) * | 1999-02-23 | 2012-05-15 | Caliper Life Sciences Inc | Manipulation von mikropartikeln in mikrofluidischen systemen |
| JP2001161400A (ja) * | 1999-12-03 | 2001-06-19 | Sumitomo Electric Ind Ltd | 固相化ｄｎａプローブによる特定遺伝子の分析法 |
| US20030207300A1 (en) * | 2000-04-28 | 2003-11-06 | Matray Tracy J. | Multiplex analytical platform using molecular tags |
| JP5115312B2 (ja) * | 2008-05-02 | 2013-01-09 | ソニー株式会社 | 核酸分離用担体の製造方法、及び核酸分離用担体とマイクロ流路系、並びに核酸分離方法と核酸分離装置 |
| JP2012018039A (ja) * | 2010-07-07 | 2012-01-26 | Sony Corp | マイクロ流路と核酸ハイブリダイゼーションのためのマイクロチップとカラム及び装置並びに方法 |
| US20130130917A1 (en) * | 2011-01-13 | 2013-05-23 | Halgen Corporation | Method for specific enrichment of nucleic acid sequences |
-
2014
- 2014-08-14 GB GBGB1414451.3A patent/GB201414451D0/en not_active Ceased
-
2015
- 2015-08-14 WO PCT/GB2015/052370 patent/WO2016024134A2/en not_active Ceased
- 2015-08-14 US US15/503,480 patent/US20170226501A1/en not_active Abandoned
- 2015-08-14 EP EP15753158.3A patent/EP3180444A2/en not_active Withdrawn
- 2015-08-14 JP JP2017508557A patent/JP2017529069A/ja active Pending
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