JP2017529069A - 核酸濃縮用のハイブリダイゼーションカラム - Google Patents

核酸濃縮用のハイブリダイゼーションカラム Download PDF

Info

Publication number
JP2017529069A
JP2017529069A JP2017508557A JP2017508557A JP2017529069A JP 2017529069 A JP2017529069 A JP 2017529069A JP 2017508557 A JP2017508557 A JP 2017508557A JP 2017508557 A JP2017508557 A JP 2017508557A JP 2017529069 A JP2017529069 A JP 2017529069A
Authority
JP
Japan
Prior art keywords
hybridization
column
nucleic acid
solid support
microbead
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017508557A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017529069A5 (https=
Inventor
ミラー，オリバー
スパイト，グラハム
サウザン，エド
マクアンドリュー，マイク
ハード，ダグラス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Gene Technology Operations Ltd
Original Assignee
Oxford Gene Technology Operations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Gene Technology Operations Ltd filed Critical Oxford Gene Technology Operations Ltd
Publication of JP2017529069A publication Critical patent/JP2017529069A/ja
Publication of JP2017529069A5 publication Critical patent/JP2017529069A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B01L2200/147—Employing temperature sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
JP2017508557A 2014-08-14 2015-08-14 核酸濃縮用のハイブリダイゼーションカラム Pending JP2017529069A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1414451.3A GB201414451D0 (en) 2014-08-14 2014-08-14 Hybridisation column for nucleic acid enrichment
GB1414451.3 2014-08-14
PCT/GB2015/052370 WO2016024134A2 (en) 2014-08-14 2015-08-14 Hybridisation column for nucleic acid enrichment

Publications (2)

Publication Number Publication Date
JP2017529069A true JP2017529069A (ja) 2017-10-05
JP2017529069A5 JP2017529069A5 (https=) 2018-09-20

Family

ID=51662437

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017508557A Pending JP2017529069A (ja) 2014-08-14 2015-08-14 核酸濃縮用のハイブリダイゼーションカラム

Country Status (5)

Country Link
US (1) US20170226501A1 (https=)
EP (1) EP3180444A2 (https=)
JP (1) JP2017529069A (https=)
GB (1) GB201414451D0 (https=)
WO (1) WO2016024134A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024534509A (ja) * 2021-09-23 2024-09-20 ジノミク テスティング コーポレーティブ，エルシーエー ｃｆＲＮＡおよびｃｆＴＮＡの標的ＮＧＳシーケンシングのための組成物および方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101799192B1 (ko) * 2016-03-24 2017-11-17 고려대학교 산학협력단 표적 유전자 검출을 위한 미세 유동 장치
EP3649260B1 (en) * 2017-07-07 2022-05-11 Nipd Genetics Public Company Limited Target-enriched multiplexed parallel analysis for assessment of tumor biomarkers
WO2019008167A1 (en) 2017-07-07 2019-01-10 Nipd Genetics Public Company Limited MULTIPLEXED PARALLEL ANALYSIS ENRICHED IN TARGET FOR RISK ASSESSMENT FOR GENETIC DISORDERS
DK3649257T3 (da) 2017-07-07 2022-07-04 Nipd Genetics Public Company Ltd Berigelse af målrettede genomiske regioner til multiplekset parallel analyse
WO2019008153A1 (en) * 2017-07-07 2019-01-10 Nipd Genetics Public Company Limited COMPLETE MULTIPLEXED PARALLEL ANALYSIS FOR THE EVALUATION OF FETAL DNA SAMPLES

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001161400A (ja) * 1999-12-03 2001-06-19 Sumitomo Electric Ind Ltd 固相化ｄｎａプローブによる特定遺伝子の分析法
US20030164335A1 (en) * 1998-10-23 2003-09-04 Grate Jay W. Method for high throughput separations in microfluidic systems using small particles
JP2012018039A (ja) * 2010-07-07 2012-01-26 Sony Corp マイクロ流路と核酸ハイブリダイゼーションのためのマイクロチップとカラム及び装置並びに方法
US20130130917A1 (en) * 2011-01-13 2013-05-23 Halgen Corporation Method for specific enrichment of nucleic acid sequences

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE424927T1 (de) * 1993-10-28 2009-03-15 Houston Advanced Res Ct Mikrofabriziertes poröses durchflussgerät zur diskreten bestimmung von bindungsreaktionen
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6969488B2 (en) * 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
DE60045917D1 (de) * 1999-02-23 2011-06-16 Caliper Life Sciences Inc Sequenzierung durch inkorporation
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
JP5115312B2 (ja) * 2008-05-02 2013-01-09 ソニー株式会社 核酸分離用担体の製造方法、及び核酸分離用担体とマイクロ流路系、並びに核酸分離方法と核酸分離装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030164335A1 (en) * 1998-10-23 2003-09-04 Grate Jay W. Method for high throughput separations in microfluidic systems using small particles
JP2001161400A (ja) * 1999-12-03 2001-06-19 Sumitomo Electric Ind Ltd 固相化ｄｎａプローブによる特定遺伝子の分析法
JP2012018039A (ja) * 2010-07-07 2012-01-26 Sony Corp マイクロ流路と核酸ハイブリダイゼーションのためのマイクロチップとカラム及び装置並びに方法
US20130130917A1 (en) * 2011-01-13 2013-05-23 Halgen Corporation Method for specific enrichment of nucleic acid sequences

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CUI X. ET AL., PLOS ONE, vol. Vol.4 Issue 1 (2009), JPN6020014410, pages 4229, ISSN: 0004254250 *
KIM J. ET AL., LANGMUIR, vol. 22(2006), JPN6019039868, pages 10130 - 10134, ISSN: 0004254249 *
OHNISHI M. ET AL., JOURNAL OF CHROMATOGRAPHY A, vol. 1109 (2006), JPN6019039866, pages 122 - 126, ISSN: 0004254248 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2024534509A (ja) * 2021-09-23 2024-09-20 ジノミク テスティング コーポレーティブ，エルシーエー ｃｆＲＮＡおよびｃｆＴＮＡの標的ＮＧＳシーケンシングのための組成物および方法

Also Published As

Publication number Publication date
US20170226501A1 (en) 2017-08-10
GB201414451D0 (en) 2014-10-01
WO2016024134A3 (en) 2016-04-07
EP3180444A2 (en) 2017-06-21
WO2016024134A2 (en) 2016-02-18

Similar Documents

Publication Publication Date Title
US20220403376A1 (en) Surface-Based Tagmentation
JP6878387B2 (ja) 固形支持体でのサンプル調製
JP5986572B2 (ja) 固定化プライマーを使用した標的ｄｎａの直接的な捕捉、増幅、および配列決定
JP2017529069A (ja) 核酸濃縮用のハイブリダイゼーションカラム
WO2019226659A1 (en) Enrichment of dna comprising target sequence of interest
CN105209639B (zh) 在固相载体扩增核酸的方法
US20080241841A1 (en) Method and apparatus for sample preparation
EP3749782B1 (en) Generation of single-stranded circular dna templates for single molecule sequencing
JP2022503873A (ja) インデックス及びバーコードを使用してアレイ上のリガンドを識別するための方法及び組成物
JP2021176302A (ja) 腫瘍のディープシークエンシングプロファイリング
US20210214781A1 (en) Measurement of nucleic acid
CN102766688A (zh) 一种检测基因序列的方法
WO2013166444A2 (en) Biomarker analysis using scodaphoresis
CN105189780A (zh) 核酸制备和分析的组合物和方法
US20240060066A1 (en) Method for the clustering of dna sequences
CN102766689A (zh) 一种增加测序读长的测序方法
CN111748621A (zh) 一种检测肺癌相关41基因的探针库、试剂盒及其应用
US20220154173A1 (en) Compositions and Methods for Preparing Nucleic Acid Sequencing Libraries Using CRISPR/CAS9 Immobilized on a Solid Support
US20240316556A1 (en) High-throughput analysis of biomolecules
JP2006508687A (ja) 固定化ゲノムｄｎａのハイブリダイゼーション方法
KR20220160661A (ko) 핵산 라이브러리를 제조하기 위한 방법 및 조성물
WO2020169666A1 (en) Improved nucleic acid target enrichment and related methods

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180810

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180810

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20190918

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20190918

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191016

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191115

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200417