JP2017537146A - Agarwood undiluted solution and its production method and use - Google Patents

Abstract

Agarwood undiluted solution comprising agarwood volatile oil and water, wherein the agarwood volatile oil is 35 to 45 wt.% Aquilaria sinensis, 25 to 35 wt. Extracted from a mixture of ˜35% by weight aquilaria. The manufacturing method includes the following steps. (1) Based on weight measurement, a mixture obtained by mixing Aquilaria sinensis, agaricus agaroca, and aquilaria washed with fresh water at a constant weight ratio to obtain a mixture; (2) Step ( The mixture of agarwood obtained in 1) is put into purified water and soaked at room temperature for 0.5 to 1.5 hours; (3) the mixture of agarwood and water in step (2) is heated to the boil, and components of agarwood volatile oil The steam containing the water passes through the cooling pipe and is cooled to form a concentrated agar liquid.

Description

The present invention relates to agarwood undiluted solution, and a production method and use thereof.

Agarwood ("Nanpaku Abe") belongs to the plant agaria Aquilaria agallocha Roxb, has an oval leaf shape, a needle shape or a reverse needle shape, and has a long tip. The inflorescence is in the form of an umbrella with no trunk or short stem. The flowers are white and the fruits are 3 cm or more. Indonesia, Cambodia, and Vietnam are the origins, but they are also cultivated in Taiwan. Agarwood is a wood that contains the Achillaria sinensis Aquilaria sinensis (lour.) Gilg and agarwood Aquilaria agallocha Roxb resin. Because the wood core is heavy and sinks and smells when placed in water, it is called agarwood. The chemicals are divided into domestic agarwood and imported agarwood, of which imported agarwood is mainly produced in areas such as India and Malaysia, while domestic agarwood, also called Hainan agarwood, is found in areas such as Hainan Island and Guangxi in China. Produced, domestic agar, or agaroca, aquilaria, its taste is bitter and bitter, slightly warm in nature, improved air flow, pain relief, warming the body, sedation and stagnation It is used in cases where the chest and abdomen are difficult to breathe, heavy or painful, gastric reversal / vomiting, renal collapse, and gastric asthma. Healthy aquilaria sinensis plants do not produce agarwood, but agarwood can be produced in aquilaria sinensis plants only by the action of natural events (lightning strikes, fires, worms, animal scratches, artificial swords, etc.) . Since agarwood contains a high amount of volatile oil, it has a strong scent, and the volatile oil is a major component of agarwood's volatile medicinal properties. The action is widely shown.

Traditional Chinese medicine (Aquilaria Resin, Aloeswood, Agarwood or Eaglewood) is a traditional and valuable medicine and natural fragrance from China, Japan, India, Southeast Asia and Middle East countries. Aquila sinensis (Lour.) G has the English name “incenseTree”, “Incense material” (Hainan), “Shiragi incense” (Guangzhou, Yunnan, Fangjiang, Shian), “Dental tree”, It is also known as “Gyokuko” (Guangdong), scented incense (herbs of herbs), blue katsuka, cliff incense, and scented incense (Guangdong). It is a medicinal plant.

Aquilaria sinensis, Pseudomonas agaroca and Aquilaria are equally belonging to the genus Astragalus, a kind of subtropical evergreen oak, and a valuable medicinal plant peculiar to China. Wood containing the resin of Aquilaria sinensis is used for medicinal purposes, the name of the chemical is agarwood, it is a source of genuine Chinese herbal agarwood, and the only plant resource that produces Kampo agarwood in China. Its utility is the same as that of wood containing imported Chinese medicine. Traditional Chinese medicine is a traditional and valuable medicine and natural fragrance in China, Japan, India and other Southeast Asian countries.

According to the description of “Herbalids”, agarwood wood has a strong antibacterial effect, and the scent enters the spleen, strengthens the spirit and fortune, supplements the five organs, coughs, expectorates, warms the stomach and spleen. Because it can be used for medicinal purposes, such as changing the airtightness and being used for pain relief, it is the finest quality medicine. The nature is a little warm, the taste is spicy and bitter. Lower your head and warm your body, warm your kidneys and help your mood. It also improves air flow, pain relief, warms the body, has anti-soreness and nausea, and makes the chest and abdomen suffocating, heavy or painful. Used in cases of asthma. The dose is 1.5-4.5g, and it is better to put it later when cooking with other Chinese medicines. The genus Agaroca contains a volatile oil and a resin, and the volatile oil contains agarospirol, which is effective for sedation. Also, when used medicinally, it has the effects of improving air flow, pain relief, warming the body, making the liver "soft", warming the kidneys and supplementing mood. Mainly corrects the following diseases: qi-asthma, vulgarism / vomiting, abdominal sensation and abdominal pain, coldness of the lower back and knees, large bowel constipation, urination of urine, decreased male semen concentration. In addition, agarwood can control the body by lowering back air, improving the stomach and spleen stagnant, warm in nature but not drying out, and improving the flow of air Does not cause diarrhea. Therefore, agarwood may be taken when a person eats raw or cold food too much and vomiting does not stop, or if he or she eats unclean food and becomes a gastrointestinal disorder. Furthermore, agarwood is a good product for the elderly, and the elderly are likely to have constipation. Therefore, constipation can be avoided by taking agarwood for a long time. When young people are busy with work, dizziness, tinnitus, and insomnia, agarwood helps them sleep well.

Agarwood acetone extract is saponified and distilled to obtain 13% volatile oil, which contains benzylacetone, methoxybenzylacetone, etc., and the residue includes hydrocinnamic acid, methoxyhydrocinnamic acid and the like. Agarwood infected by fungi is agarospirol, agarol, agarofuran, dihydroagarofuran, 4-hydroxyldihydroagarofuran, 3,4-dihydroxydihydroagarofuran. And agarose that is not infected by fungi, including sulfur, Selinane, agarol, and the like, including Nor-ketoaga-rofuran.

Aquilaria sinensis contains volatile oils and also includes agarospirol, bamuxinic acid, baimuxinal, baimu-xinol, dehydrodinoflavinol, dehydrodinoflavinol ), Ainenofuranol, β-agarofuran, dihydrocaranone, isobaimucinol sesquiterpene component. Furthermore, volatile components such as benzylacetone, methoxybenzylacetone, anisic acid, and 6-hydroxy-2- (2-phenylethyl) chromone [6-hydroxy-2- (2-phenylethyl) chromone], AH3, 6-methoxy-2- (2-phenylethyl) chromone [6-methoxy-2- (2-phenylethyl) chromone], that is, AH4,6,7-dimethoxy-2- (2-phenylethyl) chromone [6 , 7-dimethyl-2- (2-phenylethyl) chloroform], that is, AH5, 6-methoxy group-2- [2- (3'-anisole) ethyl group] chromone {6-methoxy-2- [2- (3 ' -Methoxyphenyl) ethyl] chromone} Ahbl, 2- (2-phenylethyl) chromone [2- (2-phenylethyl) chromone], that is, AH8, 6-hydroxyl-2- [2- (4'-anisole) ethyl group] chromone {6-hydroxy-2 -[2- (4'-methoxyphenyl) ethyl] ethyl} chloro}, 5,8-dihydroxy-2- (2-methoxyphenylethyl) chromone {5,8-dihydroxy-2- [2- (p-methoxyphenyl) ethyl] chromone}, 6,7-dimethoxy-2- (2-methoxyphenylethyl) chromone {6,7-dimethyl-2- [2- (p-methoxyphenyl) ethyl] chromone}, 5,8-dihydroxy-2 -(2-Phenylethyl) chromone [5,8-dihydrox 2- containing (2-phenylethyl) chromone], such as more than 2- (2-phenylethyl) chromone compound component.

Domestic agarwood contains volatile oils, and also includes agarospirol, agarol, gmelo-furan, a- and β-agarofuran, dihydroa-garofuran, Nor-ketoaga-rofuran, 4-hydroxydihydroagarofuran, 3,4-dihydroxydiagarofuran, 3,4-dihydroxyagarofuran, a-guaien ), A-brunesene, kusunol, caranone, dihydrocaronone arone), oxoagarospirol, guaia-1 (10), 11-dean-15-al [guaia-1 (10), 11-dien-15-al], serine-3,11-dean- Sesquiskies such as 9-one, 11-dien-9-one, serine-3,11-dien-9-ol, and jinkohheremol Contains terpene component. In addition, volatile components such as benzylacetone, P-methoxybenzylacetone, hydrocinnamic acid, and aqualotine, liodenine are also included. Furthermore, 2- (2-phenylethyl) chromones [2- (2-phenylethyl) chromone] and dimers, trimer components: AH1, AH1a, AH2, AH2a, Ah2b, AH3, AH4, AH5, AH6, AH7, AH8, AH9, A10, AH11, AH12, AH13, AH14, AH15, AH16, AH17, AH18, AH19b, AH19b, AH20, AH23, where AH1 is also called agarotetrol and AH2 is isoarogatrol Also called troll (isoagarotortrol).

There are usually three ways to use agarwood.
a. Use an electric furnace and place the appropriate amount of agarwood in the stainless steel pan of the electric furnace and turn on the power;
b. Use an incense burner;
c. Pour hot water into the agarwood pieces and add tea
It is a method.
When choosing agarwood pieces, it is always better to have a good quality, no coloring and sizing treatment, and especially thick. The agarwood pieces are washed with fresh water and boiled in water. The agarwood water boiled for the first time contains a lot of impurities such as dust, ant secretions such as ants, and rotting plants. The perfume must be thrown away. The agar perfume boiled for the second time may be drunk directly. In addition, tea such as puer tea and plate tea can be added using the agar perfume. Agarwood can not only remove the rotten smell of tea, it can also enhance the flavor of tea. Because the agarwood fragrance material resists water, one fragrance material may usually be soaked in water for a long period of several months.

An object of the present invention is to provide an agarwood undiluted solution, wherein the agarwood undiluted solution includes agarwood volatile oil and water, and the agarwood volatile oil is 35 to 45% by weight of Aquilaria sinensis, 25 to 35% by weight. Extracted from a mixture of 25% by weight Agaroca and 25-35% by weight aquilaria.

In a preferred embodiment of the present invention, the agarwood volatile oil of the agarwood stock solution is obtained by extraction from a mixture of 40% by weight Aquilaria sinensis, 30% by weight Agaroca and 30% by weight aquilaria. .

Another object of the present invention is to provide a method for producing agarwood concentrate, said method comprising the following steps:
(1) Based on weight measurement, a mixture of Aquilaria sinensis, agaricus agaroca, and aquilaria washed with fresh water and dried to obtain a mixture at a constant weight ratio;
(2) Put the agar mixture obtained in step (1) into purified water and soak at room temperature for 0.5 to 1.5 hours;
(3) The mixture of agarwood and purified water in step (2) is heated and boiled, and water vapor containing a component of agarwood volatile oil is cooled through a cooling pipe to form agarwood stock solution.

In one preferred embodiment of the present invention, the purified water is mineral water or purified water, and its dosage is 10 to 15 times the agarwood weight.

In one preferred embodiment of the invention, steps 2 and 3 are performed in a closed container.

As a third object of the present invention, the agar stock solution of the present invention is used for producing drinking water.

In one preferable embodiment of the present invention, the drinking water further contains a fragrance and a seasoning.

In one preferred embodiment of the invention, the drinking water further comprises a natural preservative.

The agarwood stock solution of the present invention can be used for food processing.

The agar stock solution of the present invention can be used as a raw material in processing steps that increase added value such as tea, fragrance, and essential oil.

In the agarwood undiluted solution of the present invention, three types of agarwood extracts of Aquilaria sinensis, Acera spp., And Aquilaria are included, but the chemical components of these three types of agarwood basically belong to the same type, For example, sesquiterpene compounds, 2- (2-phenylethyl) chromone, triterpenes, aromatics, fatty acids, etc. are mainly included, but there are differences in the content of the specific components of these three types of agarwood. Therefore, the agarwood undiluted solution produced using the mixture of the three kinds of agarwood not only contains the volatile oil components of the three kinds of agarwood but also has the advantage that the three kinds of agarwood extracts complement each other's strengths. Have The aroma of the agarwood undiluted solution produced using these 3 types of agarwood is deeper than the aroma of a typical agarwood extract, which has a good effect of protecting against sympathetic nerves, and in particular the effect of improving sleep. GC-MS (gas chromatogram-mass spectrum) was used to measure the components and content of the agar stock solution of the present invention. (Gas chromatogram conditions: the chromatographic column is Varian Cpsi 5CB (30 mx 0.25 nm x 0.25 nm) flexible quartz glass, the injection temperature is 250 ° C, the carrier gas is high purity nitrogen gas, and the flow rate is 1.0 ml / min. Inject without dividing the flow channel, the injection volume is 1 μl, and the temperature raising process is as follows: 90 ° C. for 4 minutes, 1 ° C./min to 150 ° C., 25 minutes, The temperature is raised to 180 ° C. at 2 ° C./min, finally raised to 230 ° C. at 2 ° C./min, and kept for 70 minutes.The mass spectral conditions are that the ion source is the EI source, the electron energy is 70 eV, the solvent Delay time is 4 minutes, mass scanning range is 50-500 m / z full scanning range),
Main components and contents are as follows.

FIG. 1 is a manufacturing flowchart of agarwood undiluted solution of the present invention.

The invention will be further described in the following examples, which are not intended to limit the scope of the invention in any way.

Example 1
Based on the measurement of weight, the mixture is obtained by mixing 40% aquilaria sinensis, 30% agaroca and 30% aquilaria after washing with fresh water to remove impurities and drying. The mixture is put in a boiling pan, 1200 times as much mineral water or purified water is added and soaked at room temperature for 1 hour, so that the beneficial components of agarwood in the mixture are sufficiently infiltrated into the water. Start the boiling device, heat and boil the mixture of the above three kinds of agarwood and water, and the volatile oil component in the agarwood enters the cooling pipe from the flow duct along with water vapor, and after cooling it is taken out from the water intake Obtain 1100 mL of agarwood stock solution.

Add fragrance and seasoning to the agarwood virgin pulp obtained by manufacturing, and after tasting, bottling, testing, and producing results that meet (food) standards, then commercialize as agarwood drinking water Can do.

Example 2
Based on the measurement of weight, it is washed with fresh water to remove impurities, and after drying, 35% of Aquilaria sinensis, 33% of Agaroca genus and 32% of Aquilaria are mixed to obtain a mixture. The mixture is put in a boiling pan, 1500 times as much mineral water or purified water is added and soaked at room temperature for 0.8 hours, so that the beneficial components of agarwood in the mixture are sufficiently infiltrated into the water. Start the boiling device, heat and boil the mixture of the above three kinds of agarwood and water, and the volatile oil component in the agarwood enters the cooling pipe from the flow duct along with water vapor, and after cooling it is taken out from the water intake Obtain 1370 mL of agarwood stock solution.

Add fragrance and seasoning to the agarwood virgin pulp obtained by manufacturing, and after tasting, bottling, testing, and producing results that meet (food) standards, then commercialize as agarwood drinking water Can do.

Example 3
Based on the measurement of weight, the mixture is obtained by mixing with 30% aquilaria sinensis, 35% agaroca genus and 35% aquilaria after washing with fresh water to remove impurities and drying. The mixture is put in a boiling pan, 1000 times the amount of mineral water or purified water is added and soaked at room temperature for 0.5 hour, thereby allowing the beneficial components of agarwood in the mixture to sufficiently penetrate into the water. Start the boiling device, heat and boil the mixture of the above three kinds of agarwood and water, and the volatile oil component in the agarwood enters the cooling pipe from the flow duct along with water vapor, and after cooling it is taken out from the water intake Obtain 855 mL of agarwood stock solution.

Add fragrance and seasoning to the agarwood virgin pulp obtained by manufacturing, and after tasting, bottling, testing, and producing results that meet (food) standards, then commercialize as agarwood drinking water Can do.

Example 4
Based on the measurement of the weight, the mixture is obtained by mixing 45% Aquilaria sinensis, 25% Agaroca and 30% Aquilaria after washing with fresh water to remove impurities and drying. The mixture is put into a boiling pan, 1100 times mineral water or purified water is added and soaked at room temperature for 1.5 hours, so that the beneficial components of agarwood in the mixture are sufficiently infiltrated into the water. Start the boiling device, heat and boil the mixture of the above three kinds of agarwood and water, and the volatile oil component in the agarwood enters the cooling pipe from the flow duct along with water vapor, and after cooling it is taken out from the water intake Obtain 960 mL of agarwood stock solution.

Add fragrance and seasoning to the agarwood virgin pulp obtained by manufacturing, and after tasting, bottling, testing, and producing results that meet (food) standards, then commercialize as agarwood drinking water Can do.

Example 5
Based on the measurement of the weight, the mixture is obtained by mixing 45% aquilaria sinensis, 30% agarica agaroca and 25% aquilaria after washing with fresh water to remove impurities and drying. The mixture is put in a boiling pan, 1400 times mineral water or purified water is added and soaked at room temperature for 1.2 hours, so that the beneficial components of agarwood in the mixture are sufficiently infiltrated into the water. Start the boiling device, heat and boil the mixture of the above three kinds of agarwood and water, and the volatile oil component in the agarwood enters the cooling pipe from the flow duct along with water vapor, and after cooling it is taken out from the water intake Obtain 1290 mL of agarwood stock solution.

Add fragrance and seasoning to the agarwood virgin pulp obtained by manufacturing, and after tasting, bottling, testing, and producing results that meet (food) standards, then commercialize as agarwood drinking water Can do.

The boiling apparatus used in the above-mentioned Examples 1 to 5 is a circular steaming pan and has a heating device at the bottom. There is a pan lid at the top, and there is a hinge device on the pan lid, so that the pan lid is divided into two parts, there is a handle in one part, the user is boiling the steaming pan in use Can be confirmed. A diversion pipe is connected to the upper end of the other portion, the diversion pipe is a pipe for discharging steam, and is connected to a cooling pipe, and an outlet of the cooling pipe is a water intake.

The agar incense solution obtained in Examples 1 to 5 described above can be used as a raw material in processing steps that increase added value, such as tea, fragrance, essential oil, and food.

Example 6
Testing sleep improvement:
Test Objective: To test whether the product has the function of improving sleep.

Materials of test: Stock solution prepared in Example 1, and stock solution of Aquilaria sinensis, A. agaroca and Aquilaria manufactured according to the method of Example 1 Instead of different types of agarwood mixture, collect the same volume of agarwood stock solution at the end without changing other conditions).

The standard for human oral intake is three times a day, 100 mL each (when the weight is 60 kg), 1.7 mL / kg · bw.

Animals to be tested: ICR cage and feed.
Breeding conditions: a barrier system having a temperature of 20 to 25 ° C. and a relative humidity of 40% to 70%.

Test methods: Direct hypnosis test, barbital sleep duration extension test, barbital hypnosis test with dose below threshold, barbital sleep latency period according to the evaluation criteria of “(China) Technical Standards for Health Food Inspection and Evaluation” (2003 edition) Perform the test. The Aquilaria sinensis undiluted solution group, the agaroca undiluted solution group, the aquilaria undiluted solution group, the agaric undiluted solution group and the negative control group (sterile water) of the present invention are installed, and the dose of each group is equivalent to 10 times the maximum standard of human dose. . A fixed dose of the test substance is given orally to the vagina three times daily, and the gavage gavage dose is 17 mL / kg · bw.

As shown in Tables 4, 5 and 6, the test results show that the oral dose to sputum is 17 mL / kg · bw, and Aquilaria sinensis stock solution, Agarica agaroca stock solution, Aquilaria stock solution, and agarwood stock solution of the present invention. Both can reduce the sleep latency period (P <0.05, 0.05, 0.05, 0.05) induced by barbital, but the agarwood concentrate of the present invention is most effective. All of the above four types of agarwood concentrates can increase the incidence of sputum sleep (P <0.05, 0.05, 0.05, 0.05) induced by barbital with a dose below the threshold, The effect of the agarwood undiluted solution of the present invention is most remarkable. All of the four types of agarwood concentrates described above can extend the sleep time (P <0.05, 0.05, 0.05, 0.05) induced by barbital, but the effect of the agarwood stock solution of the present invention is most prominent. is there. The agarwood undiluted solution produced in Example 1 has an effect of improving sleep, and the effect of improving sleep is better than the effect of any kind of agarwood at the same dose. Specific data of test results are as follows.

Table 2 shows the influence of the agarwood undiluted solution on the sleep time of cocoons induced by barbital (n = 15, x ± s).

Table 3 shows the influence (n = 15, x ± s) on the sleep incidence of sputum caused by barbital induction in which the agarwood stock solution has a dose below the threshold.

Table 4 shows the influence (n = 15, x ± s) of the agarwood undiluted solution on the sleep latency of barbs induced by barbital.

For those skilled in the art, the present invention is not limited to the details of the above-described exemplary embodiments, and the present invention can be realized in other specific forms without departing from the spirit or basic features of the present invention. It is clear that you can. Thus, in any event, it should be seen that the examples are all illustrative and not limiting. Since the scope of the present invention is limited not by the above description but by the appended claims, all changes that fall within the concept and scope of requirements equivalent to the claims are included in this application. Any drawing mark in a claim should not be construed as limiting the claim concerned.

Claims (9)

Contains agarwood volatile oil and water,
The agarwood volatile oil is obtained by extraction from a mixture of 35 to 45% by weight of Aquilaria sinensis, 25 to 35% by weight of Agaroca and 25 to 35% by weight of Aquilaria.
Agarwood stock solution characterized by that. The agarwood volatile oil is obtained by extraction from a mixture of 40% by weight Aquilaria sinensis, 30% by weight Agaroca and 30% by weight Aquilaria.
The agarwood undiluted solution according to claim 1. (1) Based on the measurement of weight, a mixture of Aquilaria sinensis, Pseudomonas agaroca and Aquilaria washed with fresh water and dried to obtain a mixture;
(2) Put the agar mixture obtained in step (1) into purified water and soak at room temperature for 0.5 to 1.5 hours;
(3) The mixture of agarwood and purified water in step (2) is heated and boiled, and water vapor containing a component of agarwood volatile oil is cooled through a cooling tube to form agarwood stock solution;
Including steps such as
The method for producing an agarwood undiluted solution according to claim 1 or 2. The purified water is mineral water or purified water, and its dose is 10 to 15 times the agarwood weight.
The manufacturing method of Claim 3 characterized by the above-mentioned. Step 2 and Step 3 are performed in a sealed container.
The manufacturing method of Claim 3 characterized by the above-mentioned.   Use of the agarwood concentrate according to claim 1 or 2 in drinking water production. The potable water further includes a fragrance and a seasoning,
7. Use according to claim 6, characterized in that The potable water further comprises a natural preservative,
7. Use according to claim 6, characterized in that Use of the agarwood concentrate according to claim 1 or 2 in food production.

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