JP2017532047A5 - - Google Patents

Info

Publication number

JP2017532047A5

JP2017532047A5 JP2017519905A JP2017519905A JP2017532047A5 JP 2017532047 A5 JP2017532047 A5 JP 2017532047A5 JP 2017519905 A JP2017519905 A JP 2017519905A JP 2017519905 A JP2017519905 A JP 2017519905A JP 2017532047 A5 JP2017532047 A5 JP 2017532047A5

Authority

JP

Japan

Prior art keywords

keratinocyte

medium

stem cells

culture

culturing

Prior art date

2015-10-13

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims 16
• 210000002510 keratinocyte Anatomy 0.000 claims 15
• 210000000130 stem cell Anatomy 0.000 claims 12
• 239000002609 medium Substances 0.000 claims 9
• 238000012258 culturing Methods 0.000 claims 6
• 210000004027 cell Anatomy 0.000 claims 4
• 210000001778 pluripotent stem cell Anatomy 0.000 claims 4
• 102000009016 Cholera Toxin Human genes 0.000 claims 3
• 108010049048 Cholera Toxin Proteins 0.000 claims 3
• 102000003971 Fibroblast Growth Factor 1 Human genes 0.000 claims 3
• 108090000386 Fibroblast Growth Factor 1 Proteins 0.000 claims 3
• 230000015572 biosynthetic process Effects 0.000 claims 3
• CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
• 102100024505 Bone morphogenetic protein 4 Human genes 0.000 claims 2
• IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical class C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 claims 2
• DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 claims 2
• 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 claims 2
• 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 claims 2
• 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims 2
• 230000011748 cell maturation Effects 0.000 claims 2
• 230000001413 cellular effect Effects 0.000 claims 2
• 229940043355 kinase inhibitor Drugs 0.000 claims 2
• 239000011159 matrix material Substances 0.000 claims 2
• 229960003966 nicotinamide Drugs 0.000 claims 2
• 235000005152 nicotinamide Nutrition 0.000 claims 2
• 239000011570 nicotinamide Substances 0.000 claims 2
• 239000003757 phosphotransferase inhibitor Substances 0.000 claims 2
• 239000002243 precursor Substances 0.000 claims 2
• 238000004114 suspension culture Methods 0.000 claims 2
• 108010049955 Bone Morphogenetic Protein 4 Proteins 0.000 claims 1
• OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
• 102000009024 Epidermal Growth Factor Human genes 0.000 claims 1
• 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims 1
• 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims 1
• 101000762379 Homo sapiens Bone morphogenetic protein 4 Proteins 0.000 claims 1
• 101000994365 Homo sapiens Integrin alpha-6 Proteins 0.000 claims 1
• 101000712674 Homo sapiens TGF-beta receptor type-1 Proteins 0.000 claims 1
• 102100032816 Integrin alpha-6 Human genes 0.000 claims 1
• 102100040445 Keratin, type I cytoskeletal 14 Human genes 0.000 claims 1
• 102100040443 Keratin, type I cytoskeletal 15 Human genes 0.000 claims 1
• 108010066321 Keratin-14 Proteins 0.000 claims 1
• 108010066330 Keratin-15 Proteins 0.000 claims 1
• 102100033456 TGF-beta receptor type-1 Human genes 0.000 claims 1
• SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims 1
• 229960005070 ascorbic acid Drugs 0.000 claims 1
• 235000010323 ascorbic acid Nutrition 0.000 claims 1
• 239000011668 ascorbic acid Substances 0.000 claims 1
• 239000007640 basal medium Substances 0.000 claims 1
• 229910052791 calcium Inorganic materials 0.000 claims 1
• 239000011575 calcium Substances 0.000 claims 1
• 230000004069 differentiation Effects 0.000 claims 1
• 210000002744 extracellular matrix Anatomy 0.000 claims 1
• 229940029303 fibroblast growth factor-1 Drugs 0.000 claims 1
• 230000000977 initiatory effect Effects 0.000 claims 1
• 239000002811 myosin light chain kinase inhibitor Substances 0.000 claims 1
• 229930002330 retinoic acid Natural products 0.000 claims 1
• 239000012679 serum free medium Substances 0.000 claims 1
• 230000023895 stem cell maintenance Effects 0.000 claims 1
• 229960001727 tretinoin Drugs 0.000 claims 1
• VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 1