JP2017518025A5 - - Google Patents
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- JP2017518025A5 JP2017518025A5 JP2016554321A JP2016554321A JP2017518025A5 JP 2017518025 A5 JP2017518025 A5 JP 2017518025A5 JP 2016554321 A JP2016554321 A JP 2016554321A JP 2016554321 A JP2016554321 A JP 2016554321A JP 2017518025 A5 JP2017518025 A5 JP 2017518025A5
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- Prior art keywords
- alpha
- sugar
- fraction
- glucosyl
- glucosidase enzyme
- Prior art date
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- 229920001503 Glucan Polymers 0.000 claims description 18
- 108010028144 alpha-Glucosidases Proteins 0.000 claims description 18
- 238000003786 synthesis reaction Methods 0.000 claims description 18
- BJHIKXHVCXFQLS-UYFOZJQFSA-N Fructose Natural products OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 claims description 16
- 239000008103 glucose Substances 0.000 claims description 14
- 239000005715 Fructose Substances 0.000 claims description 8
- 229920001542 oligosaccharide Polymers 0.000 claims description 8
- 150000002482 oligosaccharides Polymers 0.000 claims description 8
- 230000003301 hydrolyzing Effects 0.000 claims description 6
- 150000002016 disaccharides Chemical class 0.000 claims description 4
- 238000000855 fermentation Methods 0.000 claims description 4
- 230000004151 fermentation Effects 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 125000000600 disaccharide group Chemical group 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 238000010422 painting Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 description 8
- 229920000310 Alpha glucan Polymers 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 101700034878 setA Proteins 0.000 description 2
- 230000002153 concerted Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
Description
以上、本発明を要約すると以下のとおりである。
１．少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を含む糖においてアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を加水分解する方法であって、前記糖が二糖またはオリゴ糖であり、前記方法が、適切な条件下で前記糖をアルファ−グルコシダーゼ酵素と接触させることを含み、前記アルファ−グルコシダーゼ酵素が、前記糖の少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を加水分解し、前記糖の量が、前記接触前に存在した前記糖の量と比較して減少している、方法。
２．前記アルファ−グルコシダーゼ酵素が固定化されている、前記１に記載の方法。
３．加水分解前の前記糖の重合度が３〜７である、前記１に記載の方法。
４．前記接触工程後の前記糖の濃度が、前記接触前に存在した前記糖の濃度の５０％未満である、前記１に記載の方法。
５．前記適切な条件が、
（ｉ）グルカン合成反応、または
（ｉｉ）前記グルカン合成反応から得られる分画
を含み、前記糖が前記グルカン合成反応の副産物である、前記１に記載の方法。
６．前記グルカン合成反応が、少なくとも１つの不溶性アルファ−グルカン生成物を生成させる、前記５に記載の方法。
７．前記分画が前記グルカン合成反応のろ液である、前記６に記載の方法。
８．前記グルカン合成反応が、
（ｉ）グルコシルトランスフェラーゼの生成物、または
（ｉｉ）１つ以上のアルファ−１，３−グリコシド結合または１つ以上のアルファ−１，６−グリコシド結合を有するグルカンポリマーを加水分解することが可能なグルコシルトランスフェラーゼおよびアルファ−グルカノヒドロラーゼの両方の協奏作用の生成物である、少なくとも１つの可溶性アルファ−グルカン生成物を生成させる、前記５に記載の方法。
９．前記分画が前記グルカン合成反応のクロマトグラフィー分画である、前記８に記載の方法。
１０．前記アルファ−グルコシダーゼ酵素がトランスグルコシダーゼである、前記１に記載の方法。
１１．糖をアルファ−グルコシダーゼ酵素と接触させることにより生成される組成物であって、前記糖が二糖またはオリゴ糖であり、かつ少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を含み、前記酵素が、前記糖の少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を加水分解し、前記組成物が、前記接触前に存在した前記糖の量と比較して減少した量の前記糖を含む、組成物。
１２．加水分解前の前記糖の重合度が３〜７である、前記１１に記載の組成物。
１３．前記糖が（ｉ）グルカン合成反応、または（ｉｉ）前記グルカン合成反応から得られる分画にあり、前記糖が前記グルカン合成反応の副産物である、前記１１に記載の組成物。
１４．グルカン合成反応の分画に存在するフルクトースを富化する方法であって、
（ａ）グルカン合成反応から得られる分画を適切な条件下でアルファ−グルコシダーゼ酵素と接触させることであって、前記アルファ−グルコシダーゼ酵素が、前記分画内に含まれる二糖またはオリゴ糖の少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を加水分解する、接触させることと、
（ｂ）工程（ａ）の前記分画のフルクトース濃度と比較してより高いフルクトース濃度を有する組成物を得るために、工程（ａ）の前記加水分解分画からフルクトースを分離することと
を含む、方法。
１５．（ａ）グルカン合成反応から得られる分画を適切な条件下でアルファ−グルコシダーゼ酵素と接触させることであって、前記アルファ−グルコシダーゼ酵素が、前記分画内に含まれる二糖またはオリゴ糖の少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を加水分解する、接触させることと、
（ｂ）生成物を得るために微生物を用いて工程（ａ）の前記分画を発酵させることであって、前記発酵が、工程（ａ）の後または工程（ａ）と同時に行われる、発酵させることと、
（ｃ）任意選択により（ｂ）の前記生成物を単離することと
を含み、（ｂ）の前記生成物の収率が、前記アルファ−グルコシダーゼ酵素と接触していない前記グルカン合成反応の分画を発酵させる生成物の収率と比較して上昇している、発酵方法。
The present invention is summarized as follows.
1. A method of hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose bond in a sugar comprising at least one alpha-1,3 or alpha-1,6 glucosyl-glucose bond, said sugar being A sugar or an oligosaccharide, wherein the method comprises contacting the sugar with an alpha-glucosidase enzyme under suitable conditions, wherein the alpha-glucosidase enzyme is at least one alpha-1,3 or alpha of the sugar. A method wherein the 1,6-glucosyl-glucose bond is hydrolyzed and the amount of the sugar is reduced compared to the amount of the sugar present before the contact.
2. 2. The method according to 1 above, wherein the alpha-glucosidase enzyme is immobilized.
3. 2. The method according to 1 above, wherein the degree of polymerization of the sugar before hydrolysis is 3-7.
4). 2. The method according to 1, wherein the sugar concentration after the contacting step is less than 50% of the sugar concentration present before the contacting.
5. The appropriate condition is
2. The method according to 1 above, comprising (i) a glucan synthesis reaction, or (ii) a fraction obtained from the glucan synthesis reaction, wherein the sugar is a byproduct of the glucan synthesis reaction.
6). 6. The method of claim 5, wherein the glucan synthesis reaction produces at least one insoluble alpha-glucan product.
7). 7. The method according to 6 above, wherein the fraction is a filtrate of the glucan synthesis reaction.
8). The glucan synthesis reaction is
It is possible to hydrolyze a product of (i) a glucosyltransferase, or (ii) one or more alpha-1,3-glycosidic bonds or one or more alpha-1,6-glycosidic bonds 6. The method of claim 5, wherein at least one soluble alpha-glucan product is produced that is the product of the concerted action of both glucosyltransferase and alpha-glucanohydrolase.
9. 9. The method according to 8 above, wherein the fraction is a chromatographic fraction of the glucan synthesis reaction.
10. 2. The method according to 1 above, wherein the alpha-glucosidase enzyme is transglucosidase.
11. A composition produced by contacting a sugar with an alpha-glucosidase enzyme, wherein the sugar is a disaccharide or oligosaccharide and at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage Wherein the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose bond of the sugar and the composition is compared to the amount of sugar present prior to the contact. And a reduced amount of said sugar.
12 12. The composition according to 11 above, wherein the degree of polymerization of the sugar before hydrolysis is 3-7.
13. 12. The composition according to 11, wherein the sugar is (i) a glucan synthesis reaction or (ii) a fraction obtained from the glucan synthesis reaction, and the sugar is a byproduct of the glucan synthesis reaction.
14 A method for enriching fructose present in a fraction of a glucan synthesis reaction,
(A) contacting a fraction obtained from a glucan synthesis reaction with an alpha-glucosidase enzyme under appropriate conditions, wherein the alpha-glucosidase enzyme is at least a disaccharide or an oligosaccharide contained in the fraction. Hydrolyzing one alpha-1,3 or alpha-1,6 glucosyl-glucose bond, contacting;
(B) separating fructose from the hydrolyzed fraction of step (a) to obtain a composition having a higher fructose concentration compared to the fructose concentration of the fraction of step (a). ,Method.
15. (A) contacting a fraction obtained from a glucan synthesis reaction with an alpha-glucosidase enzyme under appropriate conditions, wherein the alpha-glucosidase enzyme is at least a disaccharide or an oligosaccharide contained in the fraction. Hydrolyzing one alpha-1,3 or alpha-1,6 glucosyl-glucose bond, contacting;
(B) Fermenting the fraction of step (a) with a microorganism to obtain a product, wherein the fermentation is performed after step (a) or simultaneously with step (a) And letting
(C) optionally isolating the product of (b), wherein the yield of the product of (b) is a fraction of the glucan synthesis reaction not contacted with the alpha-glucosidase enzyme. The fermentation method, which is increased compared to the yield of the product that ferments the painting.
Claims (4)
（ａ）グルカン合成反応から得られる分画を適切な条件下でアルファ−グルコシダーゼ酵素と接触させることであって、前記アルファ−グルコシダーゼ酵素が、前記分画内に含まれる二糖またはオリゴ糖の少なくとも１つのアルファ−１，３またはアルファ−１，６グルコシル−グルコース結合を加水分解する、接触させることと、
（ｂ）工程（ａ）の前記分画のフルクトース濃度と比較してより高いフルクトース濃度を有する組成物を得るために、工程（ａ）の前記加水分解分画からフルクトースを分離することと
を含む、方法。 A method for enriching fructose present in a fraction of a glucan synthesis reaction,
(A) contacting a fraction obtained from a glucan synthesis reaction with an alpha-glucosidase enzyme under appropriate conditions, wherein the alpha-glucosidase enzyme is at least a disaccharide or an oligosaccharide contained in the fraction. Hydrolyzing one alpha-1,3 or alpha-1,6 glucosyl-glucose bond, contacting;
(B) separating fructose from the hydrolyzed fraction of step (a) to obtain a composition having a higher fructose concentration compared to the fructose concentration of the fraction of step (a). ,Method.
（ｂ）生成物を得るために微生物を用いて工程（ａ）の前記分画を発酵させることであって、前記発酵が、工程（ａ）の後または工程（ａ）と同時に行われる、発酵させることと、
（ｃ）任意選択により（ｂ）の前記生成物を単離することと
を含み、（ｂ）の前記生成物の収率が、前記アルファ−グルコシダーゼ酵素と接触していない前記グルカン合成反応の分画を発酵させる生成物の収率と比較して上昇している、発酵方法。 (A) contacting a fraction obtained from a glucan synthesis reaction with an alpha-glucosidase enzyme under appropriate conditions, wherein the alpha-glucosidase enzyme is at least a disaccharide or an oligosaccharide contained in the fraction. Hydrolyzing one alpha-1,3 or alpha-1,6 glucosyl-glucose bond, contacting;
(B) Fermenting the fraction of step (a) with a microorganism to obtain a product, wherein the fermentation is performed after step (a) or simultaneously with step (a) And letting
(C) optionally isolating the product of (b), wherein the yield of the product of (b) is a fraction of the glucan synthesis reaction not contacted with the alpha-glucosidase enzyme. The fermentation method, which is increased compared to the yield of the product that ferments the painting.
Applications Claiming Priority (19)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461945241P | 2014-02-27 | 2014-02-27 | |
| US201461945233P | 2014-02-27 | 2014-02-27 | |
| US61/945,233 | 2014-02-27 | ||
| US61/945,241 | 2014-02-27 | ||
| US201462004312P | 2014-05-29 | 2014-05-29 | |
| US201462004290P | 2014-05-29 | 2014-05-29 | |
| US201462004305P | 2014-05-29 | 2014-05-29 | |
| US201462004300P | 2014-05-29 | 2014-05-29 | |
| US201462004308P | 2014-05-29 | 2014-05-29 | |
| US201462004314P | 2014-05-29 | 2014-05-29 | |
| US62/004,312 | 2014-05-29 | ||
| US62/004,308 | 2014-05-29 | ||
| US62/004,314 | 2014-05-29 | ||
| US62/004,305 | 2014-05-29 | ||
| US62/004,290 | 2014-05-29 | ||
| US62/004,300 | 2014-05-29 | ||
| CN2015073269 | 2015-02-25 | ||
| CNPCT/CN2015/073269 | 2015-02-25 | ||
| PCT/US2015/017644 WO2015130881A1 (en) | 2014-02-27 | 2015-02-26 | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017518025A JP2017518025A (en) | 2017-07-06 |
| JP2017518025A5 true JP2017518025A5 (en) | 2018-03-29 |
| JP7011393B2 JP7011393B2 (en) | 2022-02-10 |
Family
ID=52633687
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016554321A Active JP7011393B2 (en) | 2014-02-27 | 2015-02-26 | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzyme |
| JP2016554480A Active JP6929499B2 (en) | 2014-02-27 | 2015-02-26 | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzyme |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016554480A Active JP6929499B2 (en) | 2014-02-27 | 2015-02-26 | Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzyme |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP3110958A1 (en) |
| JP (2) | JP7011393B2 (en) |
| CN (2) | CN106068327A (en) |
| AU (2) | AU2015223027B2 (en) |
| BR (1) | BR112016019823B1 (en) |
| CA (2) | CA2938144A1 (en) |
| WO (2) | WO2015130883A1 (en) |
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| EP3237454B1 (en) | 2014-12-22 | 2020-08-12 | DuPont Industrial Biosciences USA, LLC | Polymeric blend containing poly alpha-1,3-glucan |
| BR112017012481B1 (en) | 2015-02-06 | 2021-10-19 | Nutrition & Biosciences USA 4, Inc. | COLLOIDAL DISPERSION, PERSONAL CARE PRODUCT, FOOD PRODUCT AND PROCESS OF MANUFACTURING A COLLOIDAL DISPERSION |
| US10294466B2 (en) | 2015-02-25 | 2019-05-21 | Danisco Us Inc. | Alpha-glucosidase, compositions and methods |
| CN107995923B (en) | 2015-06-01 | 2021-11-02 | 营养与生物科学美国4公司 | Structured liquid compositions comprising colloidal dispersions of poly alpha-1, 3-glucan |
| US11118312B2 (en) | 2015-06-01 | 2021-09-14 | Nutrition & Biosciences USA 4, Inc. | Poly alpha-1,3-glucan fibrids and uses thereof and processes to make poly alpha-1,3-glucan fibrids |
| JP7365117B2 (en) | 2015-10-26 | 2023-10-19 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | Polysaccharide coating for paper |
| US10731297B2 (en) | 2015-10-26 | 2020-08-04 | Dupont Industrial Biosciences Usa, Llc | Water insoluble alpha-(1,3-glucan) composition |
| JP6997706B2 (en) | 2015-11-13 | 2022-01-18 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | Glucan fiber composition for use in laundry care and textile care |
| US10844324B2 (en) | 2015-11-13 | 2020-11-24 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
| JP2019504932A (en) | 2015-11-13 | 2019-02-21 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ | Glucan fiber composition for use in laundry and textile care |
| US10266861B2 (en) * | 2015-12-14 | 2019-04-23 | E. I. Du Pont De Nemours And Company | Production and composition of fructose syrup |
| US10895028B2 (en) | 2015-12-14 | 2021-01-19 | Dupont Industrial Biosciences Usa, Llc | Nonwoven glucan webs |
| US20200095614A1 (en) * | 2016-12-15 | 2020-03-26 | Danisco Us Inc. | Method for increasing the production of ethanol from corn fiber in a starch hydrolysis process |
| JP2018139554A (en) * | 2017-02-28 | 2018-09-13 | 日本食品化工株式会社 | Compositions comprising water soluble dietary fiber and methods of making same |
| CN110651049A (en) | 2017-05-23 | 2020-01-03 | 纳幕尔杜邦公司 | Enzymatic production of alpha-1, 3-glucan |
| EP3415622A1 (en) * | 2017-06-14 | 2018-12-19 | Evonik Degussa GmbH | Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases |
| JP7405742B2 (en) * | 2017-09-13 | 2023-12-26 | ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド | Genetically engineered glucosyltransferase |
| JP2021502436A (en) * | 2017-11-10 | 2021-01-28 | デュポン・インダストリアル・バイオサイエンシーズ・ユーエスエイ・エルエルシー | Unique form of polysaccharide |
| US10808269B2 (en) * | 2018-02-23 | 2020-10-20 | Danisco Us Inc | Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes |
| CN115197884A (en) * | 2022-08-24 | 2022-10-18 | 吉林大学 | Microbial agent for degrading waste starch and application method thereof |
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| JP2005185182A (en) * | 2003-12-25 | 2005-07-14 | Lion Corp | METHOD FOR PRODUCING alpha-1,3-GLUCOOLIGOSACCHARIDE, THE RESULTING alpha-1,3-GLUCOOLIGOSACCHARIDE, AND PREPARATION COMPOSITION CONTAINING THE SAME |
| US20080121227A1 (en) * | 2004-09-01 | 2008-05-29 | Novozymes A/S | Liquefaction and Saccharification Process |
| WO2009152285A1 (en) * | 2008-06-11 | 2009-12-17 | Syngenta Participations Ag | Compositions and methods for producing fermentable carbohydrates in plants |
| US20100268000A1 (en) * | 2009-04-20 | 2010-10-21 | Qteros, Inc. | Compositions and Methods for Fermentation of Biomass |
| WO2010129839A1 (en) * | 2009-05-07 | 2010-11-11 | Tate & Lyle Ingredients France SAS | Compositions and methods for making alpha-(1,2)-branched alpha-(1,6) oligodextrans |
| JP2012055307A (en) * | 2010-08-09 | 2012-03-22 | Tottori Univ | Development of system for efficient ethanol production from biomass using mushroom |
| US8642757B2 (en) * | 2011-09-09 | 2014-02-04 | E I Du Pont De Nemours And Company | High titer production of highly linear poly (α 1,3 glucan) |
| US9080195B2 (en) * | 2011-09-09 | 2015-07-14 | E I Du Pont De Nemours And Company | High titer production of poly (α 1,3 glucan) |
| US8828689B2 (en) * | 2011-12-19 | 2014-09-09 | E I Du Pont De Nemours And Company | Increased poly (α 1, 3 glucan) yield using boric acid |
| WO2013096511A1 (en) * | 2011-12-19 | 2013-06-27 | E. I. Du Pont De Nemours And Company | Increased poly (alpha - 1, 3 - glucan) yield using tetraborate |
-
2015
- 2015-02-26 JP JP2016554321A patent/JP7011393B2/en active Active
- 2015-02-26 EP EP15709031.7A patent/EP3110958A1/en active Pending
- 2015-02-26 AU AU2015223027A patent/AU2015223027B2/en active Active
- 2015-02-26 EP EP15713257.2A patent/EP3110959A1/en active Pending
- 2015-02-26 AU AU2015223025A patent/AU2015223025B2/en active Active
- 2015-02-26 CN CN201580010441.2A patent/CN106068327A/en active Pending
- 2015-02-26 WO PCT/US2015/017648 patent/WO2015130883A1/en active Application Filing
- 2015-02-26 BR BR112016019823-9A patent/BR112016019823B1/en active IP Right Grant
- 2015-02-26 CN CN201580010439.5A patent/CN106460023A/en active Pending
- 2015-02-26 CA CA2938144A patent/CA2938144A1/en not_active Abandoned
- 2015-02-26 CA CA2940778A patent/CA2940778A1/en not_active Abandoned
- 2015-02-26 WO PCT/US2015/017644 patent/WO2015130881A1/en active Application Filing
- 2015-02-26 JP JP2016554480A patent/JP6929499B2/en active Active
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