CN115197884A - Microbial agent for degrading waste starch and application method thereof - Google Patents
Microbial agent for degrading waste starch and application method thereof Download PDFInfo
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- CN115197884A CN115197884A CN202211017871.5A CN202211017871A CN115197884A CN 115197884 A CN115197884 A CN 115197884A CN 202211017871 A CN202211017871 A CN 202211017871A CN 115197884 A CN115197884 A CN 115197884A
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- 229920002472 Starch Polymers 0.000 title claims abstract description 42
- 239000008107 starch Substances 0.000 title claims abstract description 42
- 235000019698 starch Nutrition 0.000 title claims abstract description 42
- 230000000593 degrading effect Effects 0.000 title claims abstract description 26
- 239000002699 waste material Substances 0.000 title claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 17
- 230000000813 microbial effect Effects 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 13
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 27
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 27
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 27
- 240000005384 Rhizopus oryzae Species 0.000 claims abstract description 27
- 235000013752 Rhizopus oryzae Nutrition 0.000 claims abstract description 27
- 241000187747 Streptomyces Species 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 6
- 239000007857 degradation product Substances 0.000 claims abstract description 6
- 230000003213 activating effect Effects 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims description 22
- 235000013379 molasses Nutrition 0.000 claims description 10
- 241001655322 Streptomycetales Species 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000187392 Streptomyces griseus Species 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 abstract description 13
- 238000006731 degradation reaction Methods 0.000 abstract description 13
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 239000003337 fertilizer Substances 0.000 abstract 1
- 239000002131 composite material Substances 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000003895 organic fertilizer Substances 0.000 description 3
- 229920000945 Amylopectin Polymers 0.000 description 2
- 229920000856 Amylose Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 2
- 102100022624 Glucoamylase Human genes 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- 229940024171 alpha-amylase Drugs 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/07—Bacillus
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/66—Aspergillus
- C12R2001/69—Aspergillus oryzae
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/845—Rhizopus
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Abstract
The invention discloses a microbial agent for degrading waste starch and an application method thereof, and the microbial agent comprises the following steps: selecting strains; activating strains; carrying out expanded culture on strains; using microbial inoculum fermentation liquor; and (4) treating the degradation product. Aspergillus oryzae, rhizopus oryzae, bacillus amyloliquefaciens and streptomyces are selected as strains, four strains with starch degradation capability are separately cultured and then mixed and fermented into a compound bacterial liquid according to a certain proportion, the compound bacterial liquid is used for degrading starch, the problems of slow degradation period and low degradation efficiency of starch substances at present are solved, the problem that starch is slowly degraded under natural conditions can be solved, the starch degradation efficiency is effectively improved, and the degraded residues have no pollution to the environment and contain rich organic matters and can be further utilized as organic fertilizers.
Description
Technical Field
The invention relates to the technical field of waste starch degradation, in particular to a microbial agent for degrading waste starch and an application method thereof.
Background
Along with the development of economy in China and the improvement of the living standard of people, people increasingly recognize the importance of recycling of biological resources, a plurality of biomasses (such as starch and cellulose) and the like are slowly degraded under natural conditions, and the recycling efficiency of the biomasses can be improved by improving the degradation efficiency of the biomasses.
Starch is a polysaccharide stored by higher plants, is a renewable natural raw material, can be degraded by microorganisms or enzymes, and is difficult to degrade without microorganisms or enzymes; some microorganisms can produce starch degrading enzymes such as alpha-amylase, glucoamylase and the like, and thus starch can be degraded by the starch degrading enzyme producing microorganism.
Disclosure of Invention
The invention aims to provide a microbial agent for degrading waste starch and an application method thereof, and aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a microbial agent for degrading waste starch comprises the following preparation steps:
1) Selecting strains;
2) Activating strains;
3) And (5) performing amplification culture on the strains.
On the basis of the technical scheme, the invention also provides the following optional technical scheme:
in one alternative: in the step 1), the strains are selected from aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021).
In one alternative: in the step 2), a Chase culture medium is selected for aspergillus oryzae (CICC 2195) and rhizopus oryzae (CICC 3153), a beef extract peptone culture medium is selected for bacillus amyloliquefaciens (CICC 10074), and a Gao's No. one culture medium is selected for streptomyces (CICC 11021) to culture.
In one alternative: in step 2), the culture temperature of the strain: aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153) and Bacillus amyloliquefaciens (CICC 10074) were cultured at 30-35 deg.C, and Streptomyces (CICC 11021) was cultured at 45-50 deg.C.
In one alternative: in the step 3), the expanding culture method of the strains comprises the following steps: preparing a molasses culture medium, respectively inoculating the activated seed liquid of the aspergillus oryzae (CICC 2195), the rhizopus oryzae (CICC 3153), the bacillus amyloliquefaciens (CICC 10074) and the streptomyces (CICC 11021) obtained in the step 2) into the molasses culture medium according to the inoculation amount of 2-20%, and carrying out expanded culture to obtain the compound fermentation liquid of the aspergillus oryzae (CICC 2195), the rhizopus oryzae (CICC 3153), the bacillus amyloliquefaciens (CICC 10074) and the streptomyces (CICC 11021).
An application method of a microbial agent for degrading waste starch comprises the following steps:
1) Using microbial inoculum fermentation liquor;
2) And (4) treating the degradation product.
In one alternative: in step 1), the fermentation liquor is used by the following method: and uniformly spraying the microbial inoculum fermentation liquor on the waste starch, and degrading for 24-144 hours at room temperature.
Compared with the prior art, the invention has the following beneficial effects:
1. the problem that the starch is slowly degraded under natural conditions can be solved, and a simple, efficient and low-cost technical method is provided for degrading starch substances in the nature.
2. Four strains with starch degradation capability are cultured separately and then mixed and fermented into a compound bacterial liquid, and the compound bacterial liquid is used for degrading starch, so that the starch degradation efficiency is effectively improved, and the problems of slow degradation period and low degradation efficiency of the existing starch substances are solved.
3. The degraded residue has no pollution to environment and contains abundant glucose and oligosaccharide, and can be further utilized.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In the embodiment of the invention, an application method of a microbial agent for degrading waste starch comprises the following specific steps:
1) Selecting strains: the strains are selected from Aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021);
2) Activation of strains: aspergillus oryzae (CICC 2195) and Rhizopus oryzae (CICC 3153) adopt a Chachi culture medium, bacillus amyloliquefaciens (CICC 10074) adopts a beef extract peptone culture medium, streptomyces (CICC 11021) adopts a Gao's I culture medium, aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153) and Bacillus amyloliquefaciens (CICC 10074) are cultured at the temperature of 30 ℃, and streptomyces (CICC 11021) is cultured at the temperature of 45 ℃;
3) And (3) expanding culture of strains: preparing a molasses culture medium, respectively inoculating the activated seed liquid of aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021) obtained in the step 2) into the molasses culture medium according to the inoculation amount of 2%, and performing expanded culture to obtain a composite fermentation liquid of the aspergillus oryzae (CICC 2195), the rhizopus oryzae (CICC 3153), the bacillus amyloliquefaciens (CICC 10074) and the streptomyces (CICC 11021);
4) The use of the microbial inoculum: uniformly spraying the composite bacteria fermentation liquor obtained in the step 3) on waste starch, and degrading for 24 hours at room temperature;
5) And (3) treatment of degradation products: and the product obtained after degradation is used as an organic fertilizer to provide a nutrient medium for crops.
Example 2
1) Selecting strains: the strains are selected from Aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021);
2) Activation of strains: aspergillus oryzae (CICC 2195) and Rhizopus oryzae (CICC 3153) adopt Chachi culture medium, bacillus amyloliquefaciens (CICC 10074) adopts beef extract peptone culture medium, streptomycete (CICC 11021) adopts Gao's No. I culture medium, aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153) and Bacillus amyloliquefaciens (CICC 10074) are cultured at 33 ℃, and streptomycete (CICC 11021) is cultured at 42 ℃;
3) And (3) expanding culture of strains: preparing a molasses culture medium, respectively inoculating the activated seed liquid of aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021) obtained in the step 2) into the molasses culture medium according to the inoculation amount of 10%, and performing expanded culture to obtain a composite fermentation liquid of aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021);
4) The use of the microbial inoculum: uniformly spraying the composite bacteria fermentation liquor obtained in the step 3) on waste starch, and degrading for 100 hours at room temperature;
5) And (3) treatment of degradation products: and the residue obtained after degradation is used as an organic fertilizer to provide a nutrient medium for crops.
Example 3
1) Selecting strains: the strains are selected from Aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021);
2) And (3) activation of strains: aspergillus oryzae (CICC 2195) and Rhizopus oryzae (CICC 3153) adopt Chachi culture medium, bacillus amyloliquefaciens (CICC 10074) adopts beef extract peptone culture medium, streptomycete (CICC 11021) adopts Gao's No. I culture medium, aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153) and Bacillus amyloliquefaciens (CICC 10074) are cultured at 35 ℃, and streptomycete (CICC 11021) is cultured at 50 ℃;
3) And (3) expanding culture of strains: preparing a molasses culture medium, respectively inoculating the activated seed liquid of aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021) obtained in the step 2) into the molasses culture medium according to the inoculation amount of 20%, and performing expanded culture to obtain a composite fermentation liquid of aspergillus oryzae (CICC 2195), rhizopus oryzae (CICC 3153), bacillus amyloliquefaciens (CICC 10074) and streptomyces (CICC 11021);
4) The use of the microbial inoculum: uniformly spraying the composite bacteria fermentation liquor obtained in the step 3 on waste starch, and degrading for 144 hours at room temperature;
5) And (3) treatment of degradation products: and the residue obtained after degradation is used as an organic fertilizer to provide a nutrient medium for crops.
The embodiment of the invention provides a microbial agent for degrading waste starch and an application method thereof, wherein the starch is polysaccharide stored by higher plants and is a renewable and biodegradable natural raw material, the naturally-occurring starch contains 20-30% of amylose and 70-80% of amylopectin, the amylose is a linear glucose polymer mainly connected by alpha-1, 4-glycosidic bonds, and the amylopectin is a branched glucose polymer generated by the alpha-1, 6-glycosidic bonds. Amylases are enzymes that hydrolyze alpha-1, 4-and alpha-1, 6-glucosidic bonds in the starch molecule to yield glucose, oligosaccharides, or dextrins, among other products. The CICC 2195 Aspergillus oryzae, CICC 10074 Bacillus amyloliquefaciens and CICC 11021 streptomyces used in the patent produce alpha amylase, and CICC 3153 Rhizopus oryzae produces glucoamylase, and the combined action of the four microorganisms can effectively degrade starch.
The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (7)
1. The microbial agent for degrading the waste starch is characterized by comprising the following preparation steps:
1) Selecting strains;
2) Activating strains;
3) And (5) performing enlarged culture of the strain.
2. The microbial agent for degrading waste starch as claimed in claim 1, wherein in the step 1), the strain is selected from aspergillus oryzae, rhizopus oryzae, bacillus amyloliquefaciens and streptomyces.
3. The microbial agent for degrading waste starch according to claim 2, wherein in the step 2), aspergillus oryzae and Rhizopus oryzae are selected from Chachi's medium, bacillus amyloliquefaciens is selected from beef extract peptone medium, and Streptomyces griseus is selected from Gao's No. one medium.
4. The microbial agent for degrading waste starch according to claim 2, wherein in the step 2), the culture temperature of the strain is: aspergillus oryzae, rhizopus oryzae and Bacillus amyloliquefaciens are cultured at 30-35 deg.C, and Streptomyces is cultured at 45-50 deg.C.
5. The microbial agent for degrading waste starch as claimed in claim 2, wherein in the step 3), the method for expanding the culture of the strain comprises: preparing a molasses culture medium, respectively inoculating the activated seed liquid of aspergillus oryzae, rhizopus oryzae, bacillus amyloliquefaciens and streptomycete obtained in the step 2) into the molasses culture medium according to the inoculation amount of 2-20%, and performing expanded culture to obtain the compound fermentation liquid of aspergillus oryzae, rhizopus oryzae, bacillus amyloliquefaciens and streptomycete.
6. The use of the microbial agent for degrading waste starch according to claims 1 to 5, comprising the steps of:
1) Using microbial inoculum fermentation liquor;
2) And (4) treating the degradation product.
7. The method for using microbial agents for degrading waste starch according to claim 6, wherein in the step 1), the fermentation broth is used by the following method: uniformly spraying the microbial inoculum fermentation liquor on the waste starch, and degrading for 24-144 hours at room temperature.
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|---|---|---|---|---|
| CN106068327A (en) * | 2014-02-27 | 2016-11-02 | 纳幕尔杜邦公司 | α glucosidase is used to come enzyme hydrolysis disaccharides and compound sugar |
| CN106701603A (en) * | 2017-03-18 | 2017-05-24 | 云南博隆生物科技开发有限公司 | Preparation method of high-efficient decay-promoting agent |
| CN113444645A (en) * | 2021-04-26 | 2021-09-28 | 上海又然生态科技有限公司 | Multifunctional composite fermentation inoculant and preparation method and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106068327A (en) * | 2014-02-27 | 2016-11-02 | 纳幕尔杜邦公司 | α glucosidase is used to come enzyme hydrolysis disaccharides and compound sugar |
| CN106701603A (en) * | 2017-03-18 | 2017-05-24 | 云南博隆生物科技开发有限公司 | Preparation method of high-efficient decay-promoting agent |
| CN113444645A (en) * | 2021-04-26 | 2021-09-28 | 上海又然生态科技有限公司 | Multifunctional composite fermentation inoculant and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 江连洲: "酶在大豆制品中的应用", 31 August 2015, 中国轻工业出版社, pages: 103 * |
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