JP2016511831A - 生物学的実体等の少なくとも一つの対象物を観察するための方法、及び関連した撮像システム - Google Patents
生物学的実体等の少なくとも一つの対象物を観察するための方法、及び関連した撮像システム Download PDFInfo
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Abstract
Description
ここで、Azは、透過率t(x,y)のフレネル変換である。
その時、再構築の数式(1)を得る可能性を与える。
Claims (11)
- 少なくとも一つの対象物(40)を観察するための方法であって、該方法は、撮像システム(20)を用いて適用されるものであって、以下の工程:
−光源(24)を用いて該対象物(単数又は複数)(40)を照射する工程(110)、
−マトリックス受光器(26)を用いて回折パターンを取得する工程(120)、を含む方法であって、
該回折パターンが、該対象物が照明方向(Z)に沿って照射される時に、該対象物(単数又は複数)(40)によって回折される波の像と一致し、
該対象物(単数又は複数)(40)、例えば生物学的実体等が、該照明方向(Z)に沿って該光源(24)と該マトリックス受光器(26)の間に配置されているものであって、
該方法が、該照射工程(110)の前に、さらに、
−該対象物(単数又は複数)(40)と接触する少なくとも一つのマーカー(50)の添加(100)を含むことを特徴とするものであって、前記又はそれぞれのマーカー(50)が、対応する対象物(40)上に結合することができるものであり、前記対象物(40)上の前記マーカー(50)の結合が、前記対象物の吸収及び光学位相シフトの中から、少なくとも一つの特徴量を増加することができる、方法。 - 前記対象物(40)上の前記マーカー(50)の結合が、少なくとも1%、好適には、少なくとも10%、前記対象物の吸収を増加することができる、請求項1に記載の方法。
- 前記対象物(40)上の前記マーカー(50)の結合が、少なくともπ/10ラジアン前記対象物の光学位相シフトを増加することができる、請求項1又は2に記載の方法。
- 前記又はそれぞれのマーカー(50)が、着色剤、蛍光剤、金属粒子及び有機粒子からなる群から選択される、請求項1〜3のいずれか1項に記載の方法。
- 前記又はそれぞれのマーカー(50)が、前記又はそれぞれの対象物(40)の対応する寸法、例えば直径の半分未満の値、好適には前記寸法の1/3未満、より好適には前記寸法の1/10未満の値の寸法、例えば直径を有する、請求項1〜4のいずれか1項に記載の方法。
- 該照明方法(Z)に沿った該対象物(単数又は複数)(40)と該マトリクス受光器(26)の間の距離(D2)が、10mm未満、好適には5mm未満である、請求項1〜5のいずれか1項に記載の方法。
- 該光源(24)が、空間的な干渉性の光源である、請求項1〜6のいずれか1項に記載の方法。
- 前記又はそれぞれの対象物(40)が、1mm未満の値、好適には100nmと100μmの間に含まれる値の寸法、例えば直径を有する、請求項1〜7のいずれか1項に記載の方法。
- 該方法が、取得工程(120)の後、該マトリックス受光器(26)によって計測される強度(I)から、再構築アルゴリズムに従って対象物(単数又は複数)(40)の光学的特性の再構築(130)工程をさらに含むものであって、該再構築アルゴリズムが、該照明方向(Z)に沿って該対象物(単数又は複数)と該マトリックス受光器(26)の間の距離(D2)に依存する、請求項1〜8のいずれか1項に記載の方法。
- 該方法は、少なくとも一つのマーカー(50)が結合される該対象物(40)を計数する工程をさらに含む、請求項1〜9のいずれか1項に記載の方法。
- −少なくとも一つの対象物(40)を照射することができる光源(24)と、
−照明方向(Z)に沿って対象物が照射される時に該対象物(単数又は複数)(40)によって回折される波の像と一致する回折パターンを取得することができるマトリックス受光器(26)と、
−該照明方向(Z)に沿って、該光源(40)と該マトリックス受光器(26)との間に配置され、生物学的実体等の該対象物(単数又は複数)を受信するための支持体(28)と、を含む撮像システム(20)であって、
該受信支持体(28)が、さらに、該対象物(単数又は複数)(40)と接触して配置される少なくとも一つのマーカー(50)を含むことを特徴づけられるものであって、前記又はそれぞれのマーカー(50)が、対応する対象物(40)上に結合することが可能であって、前記対象物(40)上の前記マーカーの結合が、前記対象物の吸収及び光学位相シフトの中から少なくとも一つの特徴的量を増加することを可能とする、撮像システム(20)。
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FR1351787A FR3002634B1 (fr) | 2013-02-28 | 2013-02-28 | Procede d'observation d'au moins un objet, tel qu'une entite biologique, et systeme d'imagerie associe |
PCT/EP2014/053986 WO2014131897A1 (fr) | 2013-02-28 | 2014-02-28 | Procédé d'observation d'au moins un objet, tel qu'une entité biologique, et système d'imagerie associé |
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EP (1) | EP2772748A1 (ja) |
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CA (1) | CA2901533A1 (ja) |
FR (1) | FR3002634B1 (ja) |
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JP2018514759A (ja) * | 2015-03-24 | 2018-06-07 | コミサリア ア レネルジ アトミク エ オウ エネルジ アルタナティヴ | 粒子の分析方法 |
JP2023519423A (ja) * | 2020-04-08 | 2023-05-10 | ビージーアイ シェンチェン | レンズレス顕微撮像システムとその方法、及び生化学物質検出システムとその方法 |
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FR3017952B1 (fr) * | 2014-02-25 | 2016-05-06 | Commissariat Energie Atomique | Procede pour determiner une concentration en lipides dans un micro-organisme. |
WO2016039722A1 (en) * | 2014-09-08 | 2016-03-17 | Leeo, Inc. | Particulate matter sensor |
FR3047077B1 (fr) | 2016-01-25 | 2020-01-10 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Procede d’observation d’un echantillon par imagerie sans lentille |
FR3054037B1 (fr) * | 2016-07-13 | 2018-08-17 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Dispositif d’observation d’un echantillon |
FR3057068B1 (fr) * | 2016-09-30 | 2020-03-20 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Dispositif d'observation d'un echantillon et procede d'observation d'un echantillon |
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CA2901533A1 (fr) | 2014-09-04 |
WO2014131897A1 (fr) | 2014-09-04 |
FR3002634A1 (fr) | 2014-08-29 |
EP2772748A1 (fr) | 2014-09-03 |
US20140248713A1 (en) | 2014-09-04 |
FR3002634B1 (fr) | 2015-04-10 |
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