JP2016508371A - ヌクレオチド配列の処理 - Google Patents
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Classifications
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
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- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00608—DNA chips
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J2219/00583—Features relative to the processes being carried out
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Abstract
Description
− 電極のアレイ、
− 関心のあるヌクレオチド配列の複製を含む少なくとも1つのナノボール、
を含み、上記ナノボールは電極に付着し、その電極には、2つ以上のそのサイズのナノボールを一度に付着させることができない。
Claims (15)
- ヌクレオチド配列の処理のための装置であって、
− 電極のアレイ、
− 関心のあるヌクレオチド配列の複製を含む少なくとも1つのナノボール、
を含み、前記ナノボールは電極に付着し、該電極には、2つ以上のそのサイズのナノボールを付着させることができない、装置。 - ヌクレオチド配列の処理のための方法であって、
関心のあるヌクレオチド配列の複製を含む少なくとも1つのナノボールが、電極のアレイのうち1つの電極に付着し、該電極には、2つ以上のそのサイズのナノボールを付着させることができない、方法。 - 前記ナノボールの内径が、関連する電極の内径の約40%よりも大きい、請求項1に記載の装置又は請求項2に記載の方法。
- 前記電極のアレイが置かれる反応チャンバを有する容器を含む、請求項1に記載の装置であって、該容器が入口を有し、該入口に、少なくとも2つの異なる試薬リザーバを選択的に連結させることができる、装置。
- 前記試薬リザーバは、異なる誘電特徴を有する溶液を含む、請求項4に記載の装置。
- 前記電極に対する選択的な電位の印加を可能にする処理回路を含む、請求項1に記載の装置。
- 前記アレイの電極が、関心のあるヌクレオチド配列の複製を含む複数のナノボールに曝露され、前記ナノボールは、実質的に該ナノボールのうち1つのみが同時に1つの電極に付着することができるようにサイズを有する、請求項1に記載の装置又は請求項2に記載の方法。
- 前記アレイの電極が、関心のあるヌクレオチド配列の複製を含む複数のナノボールに曝露され、前記電極は、個々に又は全体として扱って、表面に浮かぶ溶液から前記ナノボールを特異的に引き寄せることができる、請求項1に記載の装置又は請求項2に記載の方法。
- 前記ナノボールは、ローリングサークル増幅法によって生成される、請求項1に記載の装置又は請求項2に記載の方法。
- 電位が、前記アレイの電極に選択的に印加されて、ナノボール及び/若しくは他の成分を引き寄せる並びに/又ははね返す、請求項1に記載の装置又は請求項2に記載の方法。
- 電極の静電容量が測定されるか又は測定することができる、請求項1に記載の装置又は請求項2に記載の方法。
- 電極へのナノボールの結合が、前記電極での関連する静電容量の変化を介して検出されるか又は検出することができる、請求項11に記載の装置又は方法。
- 電極に付着するナノボールへのヌクレオチド及び/又はオリゴヌクレオチドの添加が、前記電極での関連する静電容量の変化を介して検出されるか又は検出することができる、請求項11に記載の装置又は方法。
- 前記電極のアレイは、モノヌクレオチド又はオリゴヌクレオチドの異なる溶液に順次曝露される、請求項1に記載の装置又は請求項2に記載の方法。
- 核酸を配列決定するため、分子診断のため、生物学的サンプル分析のため、化学的サンプル分析のため、食物分析のため及び/又は法医学分析のための請求項1に記載の装置の使用。
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US201361761827P | 2013-02-07 | 2013-02-07 | |
US61/761,827 | 2013-02-07 | ||
PCT/IB2014/058480 WO2014122548A2 (en) | 2013-02-07 | 2014-01-23 | Processing of nucleotide sequences |
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JP2016508371A true JP2016508371A (ja) | 2016-03-22 |
JP6430406B2 JP6430406B2 (ja) | 2018-11-28 |
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US (1) | US20150369772A1 (ja) |
EP (1) | EP2954315A2 (ja) |
JP (1) | JP6430406B2 (ja) |
KR (1) | KR20150115013A (ja) |
CN (1) | CN104969065A (ja) |
BR (1) | BR112015018674A2 (ja) |
RU (1) | RU2661784C2 (ja) |
WO (1) | WO2014122548A2 (ja) |
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CN111656179B (zh) | 2017-11-13 | 2023-11-03 | 豪夫迈·罗氏有限公司 | 用于使用表位电泳进行样品分析的装置 |
WO2020074742A1 (en) | 2018-10-12 | 2020-04-16 | F. Hoffmann-La Roche Ag | Detection methods for epitachophoresis workflow automation |
US11841310B2 (en) * | 2018-12-17 | 2023-12-12 | Illumina, Inc. | Flow cells and sequencing kits |
EP3969583A1 (en) | 2019-05-14 | 2022-03-23 | F. Hoffmann-La Roche AG | Devices and methods for sample analysis |
EP4308723A1 (en) | 2021-03-15 | 2024-01-24 | F. Hoffmann-La Roche AG | Targeted next-generation sequencing via anchored primer extension |
WO2023096674A1 (en) * | 2021-11-23 | 2023-06-01 | Pleno, Inc. | Encoded assays |
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- 2014-01-23 US US14/764,601 patent/US20150369772A1/en not_active Abandoned
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Also Published As
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US20150369772A1 (en) | 2015-12-24 |
RU2015137824A (ru) | 2017-03-14 |
CN104969065A (zh) | 2015-10-07 |
RU2661784C2 (ru) | 2018-07-19 |
BR112015018674A2 (pt) | 2017-07-18 |
WO2014122548A3 (en) | 2014-11-20 |
WO2014122548A2 (en) | 2014-08-14 |
KR20150115013A (ko) | 2015-10-13 |
EP2954315A2 (en) | 2015-12-16 |
JP6430406B2 (ja) | 2018-11-28 |
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