JP2016501382A - マイクロ流動チップベースの血小板の機能と薬物反応検査装置及び方法 - Google Patents
マイクロ流動チップベースの血小板の機能と薬物反応検査装置及び方法 Download PDFInfo
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- JP2016501382A JP2016501382A JP2015547872A JP2015547872A JP2016501382A JP 2016501382 A JP2016501382 A JP 2016501382A JP 2015547872 A JP2015547872 A JP 2015547872A JP 2015547872 A JP2015547872 A JP 2015547872A JP 2016501382 A JP2016501382 A JP 2016501382A
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Abstract
Description
11:サンプル貯留室
12:マイクロチャンネル
13a:光源
13b:光計測センサ
14:計測チャンバ
15a:光源
15b:光センサ
16:栓部
18:センサ部
20:流体駆動装置
21:リニアアクチュエータ
22:ピストン
24:シリンダ
30:A/D変換器
40:マイクロプロセッサ
Claims (20)
- 血液サンプルが収容され、前記血液サンプルの流動によって発生した血小板の付着と凝集を計測するための計測装置;及び
前記計測装置に連結され、前記血液サンプルに往復せん断流動を発生させる流体駆動装置を含むマイクロ流動チップベースの血小板の機能と薬物反応検査装置。 - 前記計測装置は、
血液サンプルが内部に収容されるサンプル貯留室;
前記のサンプル貯留室に連結され、前記血液サンプルのせん断流動を案内するマイクロチャンネル;
前記マイクロチャンネルと連結され、前記血小板の付着と凝集が発生する計測チャンバ;及び
前記計測チャンバで発生する血小板の付着と凝集を計測するためのセンサ部を含むことを特徴とする、請求項1に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。 - 前記流体駆動装置は、
リニアアクチュエータ;
前記リニアアクチュエータの駆動により直線往復運動するピストン;及び
前記ピストンの直線往復運動が可能なように設置されるシリンダを含むことを特徴とする、請求項1に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。 - 前記計測装置と流体駆動装置の間には、駆動圧力の伝達および遮断を制御するバルブが設置されることを特徴とする、請求項1に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記バルブは周期的な開閉により脈動(Pulsatile)の流動を作ることを特徴とする、請求項4に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記マイクロチャンネルは複数が並列に配置されることを特徴とする、請求項2に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記並列に配置されたマイクロチャンネルに連結された計測チャンバには異なる薬物が提供されることを特徴とする、請求項6に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記センサ部は、電気インピーダンスを測定する電極センサ、濁度を測定する光センサ、揺れ圧力を測定する圧力センサ、前記血液サンプルの往復移動距離を測定するイメージセンサの中の少なくとも一つを含むことを特徴とする、請求項2に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記電極センサは、前記計測チャンバ内の両端に2つの電極で構成され、前記計測チャンバとの間の血小板の付着と凝集に応じて変化する電気インピーダンスの変化を測定することを特徴とする、請求項8に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記光センサは、前記計測チャンバの外部に光源と対向するように構成され、前記計測チャンバとの間の血小板の付着と凝集に応じて変化する光学的濁度の変化を測定することを特徴とする、請求項8に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記圧力センサは、前記計測装置と流体駆動装置を連結するバルブ、または前記サンプル貯留室に備えられ、前記計測チャンバで血小板の付着と凝集に応じて変化する揺れ圧力変化を測定することを特徴とする、請求項8に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記イメージセンサは、
前記マイクロチャンネルに光を照射する光源;及び
前記マイクロチャンネル内の血液を透過した光を受信して電気信号に変えて血液の流れの進行距離を測定する光計測センサを含むことを特徴とする、請求項8に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。 - 前記光源はLED(Light Emitting Diode)であり、前記光計測センサはCCD(Charge Coupled Device)センサアレイであることを特徴とする、請求項12に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記計測チャンバは前記マイクロチャンネルの中央部に位置し、前記計測チャンバには前記血液サンプルの流動速度を低減させるための流路の急拡張部が形成されていることを特徴とする、請求項2に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記計測チャンバには、血小板の付着と凝集を促進し、これによる流動の減少を誘導するための複数のマイクロフィラーが設けられることを特徴とする、請求項2に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記計測チャンバの内部には、複数の玉が備えられることを特徴とする、請求項2に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記計測チャンバには、フィブリノゲン、アラキドン酸(Arachidonic acid)、コラーゲン、エピネフリン、アデノシンニリン酸(ADP)、PGE1(Prostaglandin E1)、TRAP(Thrombin Receptor Activating Peptide)、P2Y1レセプターアンタゴニスト(antagonist)、P2Y12レセプターアンタゴニストの中から選択される1種以上の薬物が提供されることを特徴とする、請求項2に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 前記P2Y1レセプターアンタゴニストはMRS2179、MRS2279、MRS2500、A2P5P、A3P5P、A3P5PSの中から選択される1種以上であり、
前記P2Y12レセプターアンタゴニストはクロピドグレル(clopidogrel)、チクロピジン(ticlopidine)、プラスグレル(prasugrel)、AR−C67085MX、カングレロル(cangrelor)、C1330−7、MRS2395、2−methylthioadenosine−5’−monophosphateの中から選択される1種以上であり、
前記薬物は、表面コーティング及び液体注入の中の少なくとも一つの形で供給されることを特徴とする、請求項17に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。 - 前記流体駆動装置によって発生するせん断流動で、最小せん断率が5000(s−1)以上であるか、最小せん断力が8Pa以上であることを特徴とする、請求項1に記載のマイクロ流動チップベースの血小板の機能と薬物反応検査装置。
- 血液サンプルを両側に配置したサンプル貯留室に注入するステップ;
前記サンプル貯留室を連結するマイクロチャンネルを介して前記血液サンプルが往復せん断流動をするようにするステップ;
前記マイクロチャンネルの中央部に位置する計測チャンバに薬物を提供して血小板と反応するようにするステップ;及び
前記計測チャンバ内で血小板の付着と凝集により発生する変化を計測するステップを含む、マイクロ流動チップベースの血小板の機能と薬物反応検査方法。
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JP2019060772A (ja) * | 2017-09-27 | 2019-04-18 | 国立大学法人東北大学 | せん断応力負荷時のフォンウィルブランド因子高分子マルチマーの保持率のインビトロ測定法 |
JP7120558B2 (ja) | 2017-09-27 | 2022-08-17 | 国立大学法人東北大学 | せん断応力負荷時のフォンウィルブランド因子高分子マルチマーの保持率のインビトロ測定法 |
JP2020060488A (ja) * | 2018-10-11 | 2020-04-16 | 国立大学法人東北大学 | 血液、血球又は凝固関連因子へ定量的にずり応力を加える剪断発生装置 |
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WO2014104761A1 (ko) | 2014-07-03 |
EP2940476A1 (en) | 2015-11-04 |
JP6091648B2 (ja) | 2017-03-08 |
CN104903728A (zh) | 2015-09-09 |
EP2940476A4 (en) | 2016-12-14 |
US9983220B2 (en) | 2018-05-29 |
KR20140094036A (ko) | 2014-07-30 |
KR101481240B1 (ko) | 2015-01-19 |
US20150338424A1 (en) | 2015-11-26 |
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