JP2016501011A - 非溶出試料の一段階核酸増幅法 - Google Patents

非溶出試料の一段階核酸増幅法 Download PDF

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Publication number
JP2016501011A
JP2016501011A JP2015541121A JP2015541121A JP2016501011A JP 2016501011 A JP2016501011 A JP 2016501011A JP 2015541121 A JP2015541121 A JP 2015541121A JP 2015541121 A JP2015541121 A JP 2015541121A JP 2016501011 A JP2016501011 A JP 2016501011A
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Japan
Prior art keywords
nucleic acid
solid support
amplification
group
guanidine
Prior art date
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JP2015541121A
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English (en)
Japanese (ja)
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JP2016501011A5 (enExample
Inventor
タトネル，ピーター・ジェームズ
ラマートン，キャサリン・ルイーズ
ピアース，アラン・スチュアート
アッシュマン，エリザベス
Original Assignee
ジーイー・ヘルスケア・ユーケイ・リミテッド
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Priority claimed from GBGB1220240.4A external-priority patent/GB201220240D0/en
Priority claimed from GBGB1301344.6A external-priority patent/GB201301344D0/en
Application filed by ジーイー・ヘルスケア・ユーケイ・リミテッド filed Critical ジーイー・ヘルスケア・ユーケイ・リミテッド
Publication of JP2016501011A publication Critical patent/JP2016501011A/ja
Publication of JP2016501011A5 publication Critical patent/JP2016501011A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2015541121A 2012-11-09 2013-11-06 非溶出試料の一段階核酸増幅法 Pending JP2016501011A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB1220240.4A GB201220240D0 (en) 2012-11-09 2012-11-09 Methods for one step nucleic acid amplification of non-eluated samples
GB1220240.4 2012-11-09
GBGB1301344.6A GB201301344D0 (en) 2013-01-25 2013-01-25 Methods for one step nucleic acid amplification of Non-Eluted samples
GB1301344.6 2013-01-25
US201361789788P 2013-03-15 2013-03-15
US61/789,788 2013-03-15
PCT/EP2013/073189 WO2014072354A1 (en) 2012-11-09 2013-11-06 Methods for one step nucleic acid amplification of non-eluted samples

Publications (2)

Publication Number Publication Date
JP2016501011A true JP2016501011A (ja) 2016-01-18
JP2016501011A5 JP2016501011A5 (enExample) 2016-12-22

Family

ID=50684085

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015541121A Pending JP2016501011A (ja) 2012-11-09 2013-11-06 非溶出試料の一段階核酸増幅法

Country Status (5)

Country Link
US (1) US20150299770A1 (enExample)
EP (1) EP2917344B1 (enExample)
JP (1) JP2016501011A (enExample)
CN (1) CN104769111B (enExample)
WO (1) WO2014072354A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016512045A (ja) * 2013-03-15 2016-04-25 ジーイー・ヘルスケア・ユーケイ・リミテッド 非溶出試料のワンステップ核酸増幅方法

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150111214A1 (en) 2013-10-17 2015-04-23 Life Technologies Corporation Direct quantification of unprocessed nucleic acid samples
US9212388B1 (en) * 2014-06-30 2015-12-15 Life Technologies Corporation Direct quantitative PCR absent minor groove binders
GB201411624D0 (en) 2014-06-30 2014-08-13 Ge Healthcare Uk Ltd Spatial molecular profiling of solid biological masses and profile storage
GB201515355D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Ltd A method and kit for analyte detection
US10000742B2 (en) 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
GB201706680D0 (en) 2017-04-27 2017-06-14 Ge Healthcare Uk Ltd Device and method for sample isolation
CN116769579A (zh) * 2018-12-04 2023-09-19 尹特根埃克斯有限公司 控制用于str分析的dna浓度
KR102789582B1 (ko) 2019-07-16 2025-03-31 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
WO2022058859A1 (en) * 2020-09-18 2022-03-24 Avant Meats Company Limited Quantification of cells embedded in a 3d scaffold

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005532827A (ja) * 2002-07-12 2005-11-04 ブリティッシュ・バイオセル・インターナショナル・リミテッド 側方フローアッセイ用のデバイスおよびその方法
JP2008099622A (ja) * 2006-10-20 2008-05-01 Japan Health Science Foundation 体液中の遺伝子増幅方法
JP2011526788A (ja) * 2008-06-30 2011-10-20 ライフ テクノロジーズ コーポレーション 粗製核酸サンプルからの直接増幅のための方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756126A (en) * 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
DE96915910T1 (de) * 1995-06-07 2004-04-22 Whatman Plc, Maidstone Trockenes festes medium zur lagerung und analyse von genetischem material
US5939259A (en) * 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6746841B1 (en) * 1999-04-14 2004-06-08 Whatman Inc. FTA- coated media for use as a molecular diagnostic tool
WO2014143714A2 (en) * 2013-03-15 2014-09-18 Ge Healthcare Uk Limited Methods for one step nucleic acid amplification of non-eluted samples

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005532827A (ja) * 2002-07-12 2005-11-04 ブリティッシュ・バイオセル・インターナショナル・リミテッド 側方フローアッセイ用のデバイスおよびその方法
JP2008099622A (ja) * 2006-10-20 2008-05-01 Japan Health Science Foundation 体液中の遺伝子増幅方法
JP2011526788A (ja) * 2008-06-30 2011-10-20 ライフ テクノロジーズ コーポレーション 粗製核酸サンプルからの直接増幅のための方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ABDELWHAB, E. M. ET AL., JOURNAL OF VIROLOGICAL METHODS, vol. 174, JPN6017031226, 2011, pages 120 - 122, ISSN: 0004015667 *
PEZZOLI, N. ET AL., LEUKEMIA RESEARCH, vol. 31, JPN6017031225, 2007, pages 1175 - 1183, ISSN: 0004015668 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016512045A (ja) * 2013-03-15 2016-04-25 ジーイー・ヘルスケア・ユーケイ・リミテッド 非溶出試料のワンステップ核酸増幅方法

Also Published As

Publication number Publication date
US20150299770A1 (en) 2015-10-22
CN104769111A (zh) 2015-07-08
EP2917344B1 (en) 2018-08-22
EP2917344A1 (en) 2015-09-16
CN104769111B (zh) 2021-04-27
WO2014072354A1 (en) 2014-05-15

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