JP2016217847A - Liquid sample inspection tool - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a liquid sample inspection tool capable of improving development precision on a development substrate.SOLUTION: A liquid sample inspection tool displays and inspects the antigen antibody reaction of a liquid sample and includes a casing for accommodating a development substrate for developing the liquid sample. The casing includes a top wall having a drop hole for dropping the sample liquid onto the development substrate. A wall section for surrounding the periphery of the drop hole is formed at the rear surface side of the top wall of the casing. A groove section directed from an inner end to an outer end at the drop hole side in an end surface is provided in part of the end surface at the development substrate side in the wall section.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a liquid sample inspection tool used when a liquid sample is inspected by immunochromatography.

  Conventionally, a liquid sample inspection tool using an immunochromatography method is known as an inspection tool such as an influenza test tool (see, for example, Patent Document 1). The liquid sample inspection tool of Patent Document 1 includes a housing, and a housing for developing a liquid sample is accommodated in the housing. The top wall of the casing of the liquid sample inspection tool of Patent Document 1 is provided with a dropping hole for a liquid sample (developing liquid) and a detection window for knowing the reaction of the liquid sample.

  In the liquid sample inspection tool of Patent Document 1, when the liquid sample dropped into the dropping hole is developed on the development substrate, if the antigen as the object to be measured is present in the liquid sample, the antigen-antibody reaction is developed. It appears as a colored marker signal on the membrane of the material, and the inspection result is confirmed by viewing this colored marker through the detection window.

JP 2010-117213 A

  By the way, the liquid sample is developed on the development base material by capillary action, and in order to obtain the required development accuracy, the liquid sample needs to be smoothly absorbed into the development base material and developed. In this type of liquid sample inspection tool, further improvement in development accuracy is required.

  Then, this invention aims at provision of the liquid sample test | inspection tool which can improve the expansion | deployment precision on a expansion | deployment base material.

  The present invention relates to a liquid sample inspection tool for displaying and inspecting an antigen-antibody reaction of a liquid sample, comprising a housing for accommodating a developing substrate for developing the liquid sample, and the case having a ceiling wall. The ceiling wall is provided with a dropping hole for dripping the liquid sample onto the developing substrate, and a wall portion surrounding the dropping hole is formed on the back surface side of the top wall of the housing, and the developing base on the wall portion is formed. A part of the end surface on the material side is provided with a groove portion from the inner end on the drip hole side toward the outer end on the end surface.

  In the liquid sample inspection tool with the above configuration, when the liquid sample dropped into the dropping hole comes into contact with the development base material, the liquid sample inspection device is developed in the development direction on the development base material. Since a groove portion from the inner end on the drip hole side to the outer end on the end surface is formed on a part of the end surface on the base material side, a part of the liquid sample dripped into the drip hole preferentially enters the groove portion. The liquid sample is transferred to the developing substrate by the groove, and the liquid sample is reliably developed in the developing direction on the developing substrate.

  In the liquid sample inspection tool of the present invention, it is possible to adopt a configuration in which the groove portion is provided along the development direction of the liquid sample, which is the longitudinal direction of the development substrate.

  As in the liquid sample inspection tool having the above-described configuration, if the groove portion is along the development direction which is the longitudinal direction of the development substrate, the liquid sample can be easily developed in the development direction.

  In the liquid sample inspection tool of the present invention, a configuration in which the width of the groove is 0.5 to 2.5 mm can be employed. By setting the width of the groove to such a dimension, the liquid sample that has entered the groove is smoothly transferred to the developing substrate.

  In the liquid sample inspection tool of the present invention, a configuration in which the depth of the groove is 0.1 to 1.0 mm can be employed. By setting the depth of the groove to such a dimension, the liquid sample that has entered the groove is smoothly transferred to the developing substrate.

  In the liquid sample inspection tool of the present invention, the end surface of the wall portion on the developing substrate side is a pressing surface that presses the developing substrate, and a configuration in which a groove is formed on the pressing surface can be employed. In this configuration, the developing base material is pressed at the end face of the wall portion on the side of the developing base material, and the developing base material is not pressed at the groove portion. Therefore, a part of the liquid sample easily enters the groove portion.

  In the liquid sample inspection tool of the present invention, the wall portion includes a weir portion that is on the opposite side of the drip hole with respect to the groove portion and protrudes from the wall portion toward the developing base material, and between the weir portion and the wall portion. A configuration in which a liquid sample reservoir is formed can be employed.

  If the wall is provided with a dam portion as in the liquid sample inspection tool having the above configuration, the liquid sample is blocked by the dam portion in the liquid sample reservoir and moves further to the opposite side in the deployment direction. This suppresses the inhibition of induction by the groove.

  The present invention is a liquid sample inspection tool for performing an inspection by displaying an antigen-antibody reaction of a liquid sample, and includes a housing that accommodates a developing substrate for developing the liquid sample, and the casing has a ceiling wall. Provided with a dropping hole for dripping the liquid sample onto the developing base material on the top wall, and a wall portion surrounding the periphery of the dropping hole is formed on the back surface side of the top wall of the housing. The end face on the base material side is provided with a space forming surface that forms a liquid sample inflow space between the end face and the development base material by being separated from the development base material with respect to other portions of the end face. Yes.

  In the liquid sample inspection tool with the above configuration, when the liquid sample dropped into the dropping hole comes into contact with the development base material, the liquid sample inspection device is developed in the development direction on the development base material. Since the end surface on the base material side is provided with a space forming surface that forms a liquid sample inflow space with the development base material by being separated from the development base material with respect to the other part of the end face, the drip hole Part of the liquid sample dropped onto the liquid sample preferentially enters the liquid sample inflow space, the liquid sample is transferred from the liquid sample inflow space to the development base material, and the liquid sample is reliably developed in the development direction on the development base material. Is done.

  According to the liquid sample inspection tool of the present invention, since the groove portion is continuously formed on the end surface on the developing substrate side in the wall portion, a part of the liquid sample dropped into the dropping hole enters the groove portion, and the groove portion Since the liquid sample that has entered is transferred from the groove portion to the developing substrate and developed in the developing direction, the developing accuracy of the liquid sample on the developing substrate can be improved.

It is a liquid sample test | inspection tool showing 1st embodiment of this invention, Comprising: It is a longitudinal cross-sectional view of the state which accommodated the expansion | deployment base material. It is a single top view of the lower housing. It is a whole single-piece top view of the liquid sample inspection tool. It is a single unit rear view of the upper case. It is a principal part enlarged view in FIG. It is the sectional view on the AA line in FIG. It is the BB sectional view taken on the line in FIG. It is a principal part expanded back view of the upper housing | casing in the liquid sample inspection tool of 2nd embodiment of this invention. It is the sectional view on the AA line in FIG. It is the BB sectional view taken on the line in FIG. It is a principal part expanded back view of the upper housing | casing in the liquid sample inspection tool of 3rd embodiment of this invention. It is the sectional view on the AA line in FIG. It is the BB sectional view taken on the line in FIG. It is a principal part expanded back view of the upper housing | casing in the liquid sample test | inspection tool of 4th embodiment of this invention. It is the sectional view on the AA line in FIG. It is the BB sectional view taken on the line in FIG.

  Hereinafter, a liquid sample inspection tool according to the present invention will be described with reference to the drawings. First, the liquid sample inspection tool according to the first embodiment of the present invention will be described. As shown in FIG. 1, the liquid sample testing tool 1 according to the first embodiment is used for mounting and holding a development substrate (also referred to as a test strip) 2 for testing the presence or absence of influenza virus infection or the like. belongs to.

  As shown in FIGS. 1 and 2, the development base material 2 is formed in an elongated rectangular sheet shape. The developing base material 2 has a sample pad 20, a membrane 22 and an absorption pad 23 in order from one end side (a sample dropping part 52 side described later) on a base card formed in a strip shape, and from the upstream side in the developing direction to the downstream in the developing direction. It is prepared on the side. Further, the developing substrate 2 has a configuration in which the end portion of the sample pad 20 is laminated on the end portion of the membrane 22 with the conjugate pad 21 sandwiched therebetween.

  When a liquid sample is dropped on the sample pad 20 from the sample dropping unit 52 on the development substrate 2, this sample penetrates into the conjugate pad 21 and a specific antibody (gold colloid, color latex, etc.) carried on the pad 21. The membrane 22 penetrates while reacting with an antibody labeled with a substance (colored label). When an antigen as an object to be measured is present in the liquid sample, this antigen and the antibody immobilized on the membrane 22 cause an antigen-antibody reaction, and a colored label appears on the membrane 22 as a signal. The inspection result can be confirmed by visually recognizing the colored marker from a detection window 57 described later, for example.

  As shown in FIG. 1, the liquid sample inspection tool 1 includes a housing 3 that accommodates a development substrate 2. The housing 3 includes a pair of divided housings. The entire housing 3 is made of synthetic resin. Hereinafter, the pair of divided housings are referred to as a lower housing 4 and an upper housing 5. The lower housing 4 is for placing the development base material 2 thereon. The upper housing 5 is a lid for the lower housing 4. Both the lower housing 4 and the upper housing 5 are formed in a rectangular plate shape having the longitudinal direction L as the same direction as the longitudinal direction L of the developing base material 2. The short direction B is a width direction orthogonal to the longitudinal direction L in a virtual plane including the longitudinal direction L. A thickness direction t (see FIG. 1) of the housing 3 is a direction orthogonal to a virtual plane including the longitudinal direction L and the short direction B. A direction from one side of the longitudinal direction to the other side of the longitudinal direction, that is, a direction from the sample pad 20 to the absorption pad 23 is a sample developing direction.

  As shown in FIGS. 1 and 2, the lower housing 4 is integrated from four sides of the bottom wall 40 at a position shifted from the peripheral end of the bottom wall 40 toward the center side in a plan view. A lower standing wall 41 is provided. The upper end surface 41a of the lower standing wall 41 is formed flat. In the liquid sample inspection tool 1 according to the present invention, the upper housing 5 and the lower housing 4 are assembled by fitting the pins 6 and the holes 7 so that the lower housing 4 and the upper housing 5 are assembled. The housing 3 is assembled. In the present embodiment, the hole 7 is disposed on the lower housing 4 side, and the pin 6 is disposed on the upper housing 5 side.

  As shown in FIG. 2, the hole 7 is formed at the center of the boss 7 </ b> A provided upright on the bottom wall 40 of the lower housing 4. In the present embodiment, the boss portions 7 </ b> A are formed at eight locations on the bottom wall 40. Specifically, the boss portions 7A are arranged at four positions on the lower standing wall 41 on one side in the short direction at intervals in the longitudinal direction L, and the longitudinal direction on the lower standing wall 41 on the other side in the short direction. It is arrange | positioned at four places at intervals in L.

  One set of the boss portions 7A is arranged to face along the short direction B. Among the boss portions 7A, the other three sets are arranged to face each other while being displaced in the longitudinal direction L. As shown in FIG. 2, the hole 7 includes a guide recess 70 and a hole 71. The guide recess 70 is formed by a pyramid surface that is inclined downward toward the lower side (the bottom wall 40 side). A hole 71 is continuously formed below the guide recess 70.

  As shown in FIG. 2, on the upper surface of the bottom wall 40 of the lower housing 4, a mounting portion for mounting the development base material 2 is formed so as to protrude. The mounting unit includes a first mounting unit 8 disposed closer to one side in the longitudinal direction, a second mounting unit 9 disposed in the middle in the longitudinal direction, and a third mounting unit disposed on the other side in the longitudinal direction. 10 is provided. The upper surface of the first mounting portion 8 is formed as a circular (or oval) flat surface, and this flat surface is provided with a mounting surface 80 for mounting one side portion (sample pad 20) in the longitudinal direction of the developing substrate 2. Has been. The second placement portion 9 and the third placement portion 10 are each composed of a plurality of ridges spaced apart in the short direction B. The second placement portion 9 is a portion on which the middle portion (conjugate pad 21 and membrane 22) of the development base material 2 in the longitudinal direction is placed. The third placement portion 10 is a portion on which the other side portion (absorption pad 23) in the longitudinal direction of the development base material 2 is placed.

  The height to the upper end of the 2nd mounting part 9 and the 3rd mounting part 10 on the basis of the upper surface of the bottom wall 40 is set equally. The second placement portion 9 is formed longer than the longitudinal length of the third placement portion 10. The height from the top surface of the bottom wall 40 to the placement surface 80 of the first placement portion 8 is slightly smaller than the height to the upper ends of the second placement portion 9 and the third placement portion 10. Is set high.

  On the bottom wall 40 of the lower housing 4, a positioning member for specifying a region for accommodating the developing base material 2 is formed so as to protrude. The positioning members are longitudinal positioning members 11 and 12 for specifying a region for accommodating the developing base material 2 in the longitudinal direction L, and a short for specifying the region for accommodating the developing base material 2 in the short direction B. Directional positioning members 13 and 14 are provided. The longitudinal positioning members 11 and 12 are erected in a pair so as to face each other in the longitudinal direction L. The longitudinal positioning members 11 and 12 are formed in a wall shape along the short direction B. One longitudinal positioning member 11 of the longitudinal positioning members 11 and 12 is disposed closer to one side in the longitudinal direction than the first placement portion 8. The other longitudinal direction positioning member 12 is disposed closer to the other side in the longitudinal direction with respect to the third placement portion 10. The height to the upper end of the longitudinal positioning members 11 and 12 with respect to the upper surface of the bottom wall 40 is set higher than the height of the mounting surface 80 of the first mounting portion 8.

  The short direction positioning members 13 and 14 are erected in pairs so as to face each other in the short direction B. Two sets of the short direction positioning members 13 and 14 are provided apart from each other in the longitudinal direction L. The lateral direction positioning members 13 and 14 are formed in a wall shape along the longitudinal direction L. The lateral direction positioning member 13 on one side in the longitudinal direction is disposed between the first placement portion 8 and the second placement portion 9. The short side direction positioning member 14 on the other side in the longitudinal direction is disposed between the second placement portion 9 and the third placement portion 10. The height to the upper ends of the lateral direction positioning members 13 and 14 with respect to the upper surface of the bottom wall 40 is set to be the same as the longitudinal direction positioning members 11 and 12.

  As shown in FIGS. 1 and 3, the upper housing 5 is integrated with the top wall 50 formed in a rectangular shape in plan view, and at the peripheral edge (edge) of the top wall 50 from the four sides of the top wall 50. And an upper standing wall 51 that is erected so as to hang down. In a state where the lower housing 4 and the upper housing 5 are assembled, the lower standing wall 41 is inside the upper standing wall 51 and faces in the longitudinal direction L and the short direction B.

  As shown in FIG. 4, the pin 6 that fits into the hole 71 is integrally formed on the back surface of the top wall 50 inside the upper standing wall 51 in the upper housing 5 so as to protrude. The pin 6 is disposed at a position corresponding to the hole 71. As shown in FIGS. 6 and 7, each pin 6 has a body 60, a head 61 formed larger in diameter than the body 60, and further extended from the head, compared to the head 61. And a small-diameter tip 63. Further, the pin 6 is reinforced by an inclined reinforcing rib 64 integrally formed on the back surface of the top wall 50. The reinforcing rib 64 is set such that the height dimension and the separation dimension enter the guide recess 70 of the hole 7.

  The maximum height of the pin 6 from the back surface of the top wall 50 of the upper housing 5, that is, the height from the back surface of the top wall 50 to the end of the tip 63 is the maximum depth of the hole 7, that is, the boss. The depth is set smaller than the depth from the upper end of the portion 7A to the bottom surface of the hole 71. In other words, when the pin 6 including the reinforcing rib 64 is fitted into the hole 7, a dimensional relationship that creates a gap between the end of the tip 63 of the pin 6 and the bottom surface of the hole 71 is set. ing.

  As shown in FIGS. 1 and 3, the sample dropping unit 52 for dropping a liquid sample is formed in a region corresponding to the sample pad 20 of the developing base material 2 on the top wall (upper surface) 50 of the upper housing 5. Is formed. An elliptical dropping hole 53 is formed in the sample dropping part 52, and an outer peripheral region of the dropping hole 53 is an inclined wall (corresponding to a wall part) 54 that is inclined toward the dropping hole 53. The upper surface of the inclined wall 54 is an upper inclined surface 54a. As shown in FIGS. 1 and 6, the region corresponding to the upper inclined surface 54 a on the back surface of the top wall 50 of the upper housing 5 is a lower inclined surface 54 b inclined downward so as to follow the upper inclined surface 54 a. The lower end portion of the inclined wall 54 is a flat surface parallel to the top wall 50. This flat surface is a pressing surface 55 that presses the sample pad 20 from above.

  The lower end portion of the inclined wall 54 is a lower wall portion 54A that surrounds the periphery of the dripping hole 53, and the inner peripheral surface of the lower wall portion 54A is a surface along the thickness direction t. An end surface of the lower wall portion 54 </ b> A on the developing base material 2 side is the pressing surface 55, and a groove portion 53 </ b> A directed in the developing direction is continuous with the dropping hole 53 at a part corresponding to the developing direction side. (See, for example, FIG. 5). The groove 53A is guiding means for guiding the liquid sample to be developed in the development direction. In this case, “toward the development direction” means a form extending in the development direction, and the groove 53A is formed to extend from the inner end to the outer end on the drip hole 53 side.

  Specifically, the groove 53A is formed along the developing direction of the liquid sample, and the groove 53A is arranged on the pressing surface 55 at the center in the width direction orthogonal to the developing direction. The groove 53A is formed to a depth that does not reach the height of the lower wall 54A. That is, the groove 53A is set to a height (depth) smaller than the height corresponding to the thickness direction t of the inner peripheral surface of the lower wall portion 54A. In other words, the groove 53 </ b> A is formed to a depth that does not reach the height of the dropping hole 53 with respect to the pressing surface 55. The wall surface that forms the groove 53 </ b> A is formed in an arc shape, and the wall surface is formed in parallel with the pressing surface 55. The position of the apex of the arc-shaped wall surface that forms the groove 53A corresponds to the height (depth) of the groove 53A. The width of the groove 53A is set in the range of 0.5 to 2.5 mm. The depth of the groove 53A is set in the range of 0.1 to 1.0 mm. In the present embodiment, the groove 53A has a width of 1.5 mm and a depth of 0.5 mm.

  A detection unit 56 is formed in a region of the top wall 50 of the upper housing 5 corresponding to the membrane 22 of the development base material 2. A rectangular detection window 57 is formed in the detection unit 56. The detection window 57 is an opening for knowing the reaction of the liquid sample on the development substrate 2. The detection window 57 is arranged away from the dropping hole 53 in a direction corresponding to the longitudinal direction of the developing substrate, which is the developing direction of the liquid sample. An outer peripheral region of the detection window 57 is an inclined surface 58 that is inclined toward the detection window 57. On the back surface of the top wall 50 of the upper housing 5, a region corresponding to the inclined surface 58 is inclined downward so as to follow the inclined surface 58, and a lower end portion thereof is a flat surface. The flat surface is a membrane pressing surface 59 that presses the membrane 22.

  On the back surface of the top wall 50 of the upper housing 5, a pressing wall 15 for pressing the conjugate pad 21 of the development base material 2 is formed so as to protrude closer to one side in the longitudinal direction of the membrane pressing surface 59. . The pressing wall 15 is a wall that is long in the short-side direction B, and the lower surface thereof is a flat surface. The flat surface is a conjugate pad pressing surface 150 that presses the conjugate pad 21. The top wall 50 of the upper housing 5 is integrally formed with a sample dropping number instruction display part, an infection presence / absence result display part, and an end confirmation display part (not shown) by engraving or the like at the time of molding. .

  The housing 3 in the present embodiment is configured by assembling the upper housing 5 and the lower housing 4 having the above-described configuration. Specifically, first, in the assembling work, for example, an operator places the developing base material 2 on the lower housing 4. At this time, the operator places the developing substrate 2 so as to be inserted between the longitudinal positioning members 11 and 12 and the lateral positioning members 13 and 14. In other words, in order to place the development base material 2, the development base material 2 can be placed using the longitudinal direction positioning members 11, 12 and the short direction positioning members 13, 14 as marks.

  When the deployment base material 2 has been installed in the lower casing 4, the lower casing 4 and the upper casing 5 are assembled so that the upper side of the lower casing 4 is covered with the upper casing 5. At this time, the operator tries to fit each pin 6 and each hole 7, but one set of the boss portions 7 </ b> A is arranged to face along the short direction B. However, among the boss portions 7A, the other three sets are arranged to face each other while being displaced in the longitudinal direction L.

  When the boss portion 7A is disposed so as not to be displaced, even if the operator mistakes the longitudinal direction L of the lower housing 4 and the longitudinal direction L of the upper housing 5, each pin 6 and each hole portion 7 can be fitted, so there is a possibility that the upper housing 5 is assembled in the wrong direction with respect to the lower housing 4. However, in this embodiment, even if the operator mistakes the longitudinal direction L of the lower housing 4 and the longitudinal direction L of the upper housing 5, the pins 6 and the holes 7 are misaligned and cannot be fitted. Therefore, an appropriate liquid sample inspection tool 1 can be manufactured without assembling the longitudinal direction L of the lower housing 4 and the longitudinal direction L of the upper housing 5 in an incorrect state.

  If the sample pad 20 is compressed too much by the pressing surface 55, the sample is difficult to flow, and if there is an excessive gap between the pressing surface 55 and the sample pad 20, the liquid sample leaks from the sample pad 20. The conjugate pad pressing surface 150 is configured to suppress the floating of the membrane 22 of the developing substrate 2 and the conjugated pad 21. The membrane pressing surface 59 prevents the membrane 22 and the absorption pad 23 of the development substrate 2 from floating so that the flow of the liquid sample does not deteriorate. )

  By fitting the head 61 of the pin 6 into the hole 7, the upper housing 5 does not loosen with respect to the lower housing 4. For this reason, the development base material 2 can be reliably pressed at a predetermined position in the housing 3, and the liquid sample dropped on the development base material 2 is developed while being surely infiltrated, and an accurate and prompt inspection result is obtained. can get. In addition, the sample pad 20 placed on the placement surface 80 is pressed by the pressing surface 55 which is a holding portion, and the conjugated pad 21 placed on the second placement portion 9 is the conjugate pad which is the holding portion. The membrane 22 that is pressed by the pressing surface 150 and placed on the second placement portion 9 is pressed by the membrane pressing surface 59 that is a pressing portion. Thereby, since the expansion | deployment base material 2 hold | maintains an exact position in the longitudinal direction L, it becomes the structure from which an exact and quick test result can be obtained.

  With the configuration as described above, in the liquid sample inspection tool 1 according to the present embodiment, when a liquid sample is dropped into the dropping hole 53, the liquid sample is absorbed by the developing base material 2 by capillary action, and the developing base material 2 over time. Expanded to As described above, when an antigen as an object to be measured is present in a liquid sample, an antigen-antibody reaction occurs between the antigen and an antibody fixed on the membrane 22, and a colored label appears on the membrane 22 as a signal. The inspection result can be confirmed by visually recognizing the colored mark from the detection window 57.

  By the way, although the liquid sample dropped on the developing substrate 2 is developed on the developing substrate 2, in the liquid sample inspection tool 1 of this embodiment, the liquid sample is more reliably developed to improve the developing accuracy. Are provided with guiding means. As described above, this guiding means is the groove 53A.

  That is, when the liquid sample dropped into the dropping hole 53 comes into contact with the developing substrate 2, it is developed in the developing direction on the developing substrate 2, while the lower wall portion 54A has a lower wall portion on the end surface on the developing substrate 2 side. Since the groove portion 53A extending in the developing direction is continuously formed in the dropping hole 53 in 54A, a part of the liquid sample dropped into the dropping hole 53 preferentially enters the groove portion 53A. Since the groove portion 53A has a depth that does not reach the height of the lower wall portion 54A, the bottom of the groove portion 53A approaches the development base material 2 by that amount, and the liquid sample that has entered the groove portion 53A reaches the development base material 2. Easy to move. Then, the liquid sample that has entered the groove 53A is transferred from the groove 53A to the developing substrate 2 by capillary action, and is developed in the developing direction. As a result, the liquid sample can be developed more reliably.

  Further, the groove 53A is provided along the developing direction of the liquid sample, and is disposed at the center in the width direction. With this configuration, the groove portion 53A can assist the development of the liquid sample so that the liquid sample can be easily developed, and further, the development is performed so as to spread uniformly from the center in the width direction to both sides in the width direction. .

  Moreover, the width of the groove 53A is 0.5 to 2.5 mm, and the liquid sample that has entered the groove 53A is smoothly transferred to the developing substrate 2 by setting the width of the groove 53A to such a dimension. I found out. Furthermore, it was found that the liquid sample that entered the groove 53A was smoothly transferred to the development substrate 2 by setting the depth of the groove 53A to 0.1 to 1.0 mm.

  By the way, in order for the liquid sample to be smoothly spread on the spreading substrate 2, in the first place, the amount of the liquid sample dropped into the dropping hole 53 is appropriate, the dropping speed is appropriate, Various conditions such as appropriate viscosity are involved. For example, if 3 drops are specified as the drop volume of the liquid sample, 4 drops or 5 drops may be dropped, or the drop speed may be too fast (from drop to next If the time until dropping is too short), until now, the liquid sample may leak from the gap between the assembly surfaces of the upper housing 5 and the lower housing 4. If the liquid sample leaks from the inside of the liquid sample inspection tool 1, a development failure of the liquid sample on the development substrate 2 occurs, and an accurate inspection cannot be performed. When the liquid sample leaks from the gap between the assembly surfaces of the upper housing 5 and the lower housing 4, the liquid sample that could not be absorbed by the developing base material 2 may be absorbed by the top of the upper housing 5 due to capillary action. There is a case where a phenomenon occurs in which the air leaks from the gap between the assembly surfaces along the back surface of the wall 50.

  On the other hand, the liquid sample inspection tool 1 of the present embodiment includes a groove 53A. For this reason, even when the dropping amount of the liquid sample is too large or the dropping speed is too high, the liquid sample is guided to the developing substrate 2 by the guide of the groove 53A, and moved on the developing substrate 2 in the developing direction. It is. For this reason, the movement of the liquid sample to the gap between the assembly surfaces of the upper housing 5 and the lower housing 4 can be suppressed to prevent leakage of the liquid sample, and the liquid sample can move in a direction other than the original deployment direction. Therefore, the absolute amount of the liquid sample developed on the development substrate 2 increases.

  As described above, in the liquid sample inspection tool 1 of the present embodiment, even when the conditions for dropping the liquid sample are not appropriate, the liquid sample is effectively prevented from leaking to the outside, and the development accuracy is improved. Be made. Further, by improving the development accuracy, development failures can be suppressed and false negatives can be avoided.

  The liquid sample inspection tool 1 according to the present invention is not limited to the above embodiment, and various modifications can be made without departing from the gist of the present invention. 8, 9 and 10 show a second embodiment of the present invention. In the liquid sample inspection tool 1 according to the second embodiment, a weir portion 54B that protrudes downward (toward the development substrate 2 side) is provided in a portion of the inclined wall 54 that corresponds to a position that avoids the groove portion 53A. ing. The weir portion 54B is formed integrally with the inclined wall 54. Specifically, the dam portion 54B is a wall formed in the middle of the inclined direction of the lower inclined surface 54b of the inclined wall 54 so as to be erected downward. The lower end surface of the weir portion 54B is at a height position flush with the pressing surface 55. This dam portion 54B is a guidance assisting means for assisting the guidance in the groove 53A (guidance means). The weir portion 54B has a region that is detached from the development base material 2. In this case, it is dislocated in the width direction B with respect to the development base material 2.

  The weir portion 54B extends along the circumferential direction of the outer peripheral surface (lower inclined surface 54b) of the inclined wall 54, and is provided to the extent that the inclined wall 54 is half-circulated on the opposite side in the development direction with respect to the groove portion 53A. In other words, the weir portion 54B is formed in a horseshoe shape with its development direction side opened. Moreover, the cross section of the dam part 54B is formed in the rectangle (trapezoid shape).

  The inner peripheral surface 514 of the weir portion 54B is used as guidance assisting means for guiding and assisting the development of the liquid sample in the deployment direction together with the groove portion 53A. That is, by forming the dam portion 54B, a liquid sample reservoir 516 is formed between the inner peripheral surface 514 of the dam portion 54B and the outer peripheral surface of the inclined wall 54. The inner peripheral surface 514 is a surface that follows the vertical direction. Since other configurations are the same as those of the first embodiment, components having the same functions are denoted by reference numerals, and description thereof will not be repeated.

  According to the liquid sample inspection tool 1 according to the second embodiment, particularly when the amount of liquid sample dripped is too large, a part of the liquid sample dripped into the drip hole 53 is blocked by the dam portion 54B. The liquid sample pool 516 is collected. If the liquid sample is accumulated in the liquid sample reservoir 516, a phenomenon that the liquid sample further travels on the back surface of the top wall 50 of the upper housing 5 and leaks from a gap between the assembling surfaces can be effectively suppressed. Further, since the liquid sample travels along the back surface of the ceiling wall 50, it is possible to suppress the liquid sample from being obstructed from flowing in the original deployment direction.

  A part of the liquid sample once stored in the liquid sample reservoir 516 moves in the developing direction on the developing base material 2 as the liquid sample on the developing base material 2 is developed. Therefore, the absolute amount of the liquid sample developed on the development substrate 2 increases, and the development accuracy can be improved more reliably.

  FIG. 11, FIG. 12, and FIG. 13 show a third embodiment. In the liquid sample inspection tool 1 according to the third embodiment, the dam portion 54B has a cross-sectional shape. In the second embodiment, the outer peripheral surface of the dam portion 54B is an inclined surface that is inclined radially outward toward the upper side. Has been. The base end of the outer peripheral surface 515 of the weir portion 54B substantially coincides with the base end of the inclined wall 54. The inner peripheral surface 514 of the weir portion 54B is a surface that follows the vertical direction.

  Since other configurations are the same as those of the second embodiment, components having the same functions are denoted by the same reference numerals, and description thereof is not repeated. Moreover, the liquid sample test | inspection tool 1 in 3rd embodiment can show | play the effect similar to the liquid sample test | inspection tool 1 in 2nd embodiment.

  FIG. 14, FIG. 15 and FIG. 16 show a fourth embodiment. The liquid sample tester 1 according to the fourth embodiment differs from the liquid sample tester 1 of the second and third embodiments in the position of the weir part 54B and the height of the weir part 54B.

  In the liquid sample inspection tool 1 according to the fourth embodiment, the weir portion 54B (the inner peripheral surface 514) thereof is the proximal end in the inclination direction of the inclined wall 54, as compared with the liquid sample inspection tool 1 according to the first embodiment. Arranged on the side. In the second embodiment, the lower end surface of the weir portion 54B is flush with the pressing surface 55, whereas in the fourth embodiment, the lower end surface of the weir portion 54B is compared to the pressing surface 55. It is arranged above. That is, the height of the weir portion 54B is lower than that in the second embodiment. Even if the weir portion 54B is low, the liquid sample reservoir 516 is formed, so that a part of the liquid sample stored in the liquid sample reservoir 516 is developed with the development of the liquid sample on the development substrate 2. Since the absolute amount of the liquid sample that moves in the developing direction on 2 and developed on the developing substrate 2 increases, the developing accuracy can be improved.

  In each of the above embodiments, the case where the groove 53A is formed so as to extend from the inner end to the outer end on the drip hole 53 side is illustrated. However, the present invention is not limited to this configuration, and may be formed from the inner end on the drip hole 53 side toward the outer end of the wall portion (for example, the inclined wall 54). You may form so that it may not reach an outer end toward an outer end from an end. Even in this configuration, as in the above embodiments, a part of the liquid sample dropped into the dropping hole 53 preferentially enters the groove 53A and is transferred from the groove 53A to the developing substrate 2 by capillary action. And expanded in the expansion direction. As a result, the liquid sample can be reliably developed.

  In each said embodiment, the case where the wall part was made into the inclined wall was illustrated. However, the wall portion is not limited to the inclined configuration, and although not shown, the wall portion may have a configuration along the vertical direction, and in this case, the present invention can be applied. Specifically, in the configuration in which the wall portion is formed in a cylindrical shape surrounding the dropping hole, the groove portion is formed in a part of the lower end surface (pressing surface). When the dam portion is provided, the dam portion is provided so as to form a liquid sample reservoir in the middle of the wall portion in the height direction (thickness direction t) or the base end portion of the wall portion.

  In each of the above embodiments, the groove 53A is formed along the developing direction of the liquid sample, and the groove 53A is arranged at the center of the pressing surface 55 in the width direction orthogonal to the developing direction, and the height of the lower wall portion 54A. The case where the wall surface forming the groove 53A is formed in an arc shape and the wall surface is formed in parallel with the pressing surface 55 is illustrated.

  However, a plurality of groove portions may be arranged by being displaced at the center of the pressing surface in the width direction or by being displaced from one side in the width direction or both sides in the width direction with respect to the center in the width direction. Moreover, the depth corresponding to the back surface of a top wall may be sufficient if the depth of a groove part is in the range of 0.1-1.0 mm. Further, the wall surface of the groove portion may not be parallel to the pressing surface but may be inclined upward or downward in the developing direction with respect to the pressing surface. Furthermore, the direction of the groove portion does not need to be along the development direction (longitudinal direction of the development substrate 2), and can be formed in a direction inclined with respect to the development direction.

  The casing 3 is formed in an elongated rectangular shape, but may be formed in an elliptical shape. Further, the groove portion of the liquid sample inspection tool 1 is arranged at the center in the width direction orthogonal to the longitudinal direction of the developing substrate, and the dropping hole is formed in a circular shape or an oval shape with its center aligned with the center in the width direction of the groove portion. Was adopted. However, the present invention is not limited to this configuration, and a rectangular shape or a triangular shape can also be used. Furthermore, the use of the present liquid sample inspection tool 1 is not limited to the presence or absence of influenza virus infection, but can also be used for the presence or absence of various virus infections, fecal occult blood, and the like.

  Although not shown, a groove (single or plural) is formed on the back surface of the ceiling wall 50 on the back side of the drop hole 53 so as to guide the liquid sample that has passed through the groove 53A to the detection window 57 side. You can also The liquid sample can be prevented from obstructing the flow of the liquid sample in the original deployment direction by being transmitted through the back surface of the top wall 50 so as to be guided by the groove.

  Furthermore, in the liquid sample inspection tool of the present invention, the end surface of the wall portion on the side of the developing base material 2 is separated from the developing base material 2 with respect to the other part of the end surface, so that it is between the developing base material 2. You may provide the space formation surface which forms a liquid sample inflow space. Specifically, as a space forming surface, the pressure surface 55 is inclined upward in the development direction, so that the height increases between the pressure surface 55 and the upper surface of the development base material 2 toward the development direction side. A liquid sample inflow space is formed. The liquid sample that has flowed into the liquid sample inflow space is transferred to the developing substrate 2 in the same manner as the groove 53A of each of the above embodiments. The space forming surface may be a surface that is continuously inclined upward in the development direction, or may be a stepped surface.

  DESCRIPTION OF SYMBOLS 1 ... Liquid sample test tool, 2 ... Development | deployment base material, 3 ... Housing | casing, 4 ... Lower housing | casing, 5 ... Upper housing | casing, 20 ... Sample pad, 21 ... Conjugate pad, 22 ... Membrane, 23 ... Absorption pad, DESCRIPTION OF SYMBOLS 50 ... Top wall, 52 ... Sample dropping part, 53 ... Dropping hole, 53A ... Groove part, 54 ... Inclined wall, 54A ... Lower wall part, 54B ... Weir part, 54a ... Upper inclined surface, 54b ... Lower inclined surface, 57 ... Detection window, 514 ... inner peripheral surface, 515 ... outer peripheral surface, 516 ... liquid sample reservoir, B ... short side direction, L ... long side direction, t ... thickness direction

Claims (8)

A liquid sample tester for displaying and inspecting an antigen-antibody reaction of a liquid sample,
A housing that houses a developing substrate for developing a liquid sample,
The housing includes a ceiling wall, and the ceiling wall includes a dropping hole for dropping a liquid sample onto the developing substrate,
A wall portion surrounding the periphery of the dropping hole is formed on the back side of the top wall of the housing,
A liquid sample inspection tool, wherein a groove portion is provided in a part of an end surface on the developing substrate side of the wall portion from an inner end on the drip hole side toward an outer end of the end surface.   The liquid sample inspection tool according to claim 1, wherein the groove portion is provided so as to extend from the inner end to the outer end on the dropping hole side in the end face.   The liquid sample inspection tool according to claim 1, wherein the groove portion is provided so as to be along a development direction of the liquid sample which is a longitudinal direction of the development base material.   The liquid sample inspection tool according to any one of claims 1 to 3, wherein the groove has a width of 0.5 to 2.5 mm.   The liquid sample inspection tool according to any one of claims 1 to 4, wherein a depth of the groove portion is 0.1 to 1.0 mm.   The liquid sample inspection tool according to any one of claims 1 to 5, wherein an end surface of the wall portion on the side of the developing substrate is a pressing surface that presses the developing substrate, and a groove is formed on the pressing surface. .   A wall provided with a dam part on the opposite side of the drip hole with respect to the groove part and projecting from the wall part toward the developing base material, and a liquid sample reservoir is formed between the dam part and the wall part. The liquid sample inspection tool according to any one of claims 1 to 6. A liquid sample tester for displaying and inspecting an antigen-antibody reaction of a liquid sample,
A housing that houses a developing substrate for developing a liquid sample,
The housing includes a ceiling wall, and the ceiling wall includes a dropping hole for dropping a liquid sample onto the developing substrate,
A wall portion surrounding the periphery of the dropping hole is formed on the back side of the top wall of the housing,
The end surface on the developing substrate side of the wall portion includes a space forming surface that forms a liquid sample inflow space with the developing substrate by being separated from the developing substrate with respect to other portions of the end surface. A liquid sample inspection tool characterized by that.

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