CN109425573B - Solution detection device and detection method - Google Patents
Solution detection device and detection method Download PDFInfo
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- CN109425573B CN109425573B CN201710770834.4A CN201710770834A CN109425573B CN 109425573 B CN109425573 B CN 109425573B CN 201710770834 A CN201710770834 A CN 201710770834A CN 109425573 B CN109425573 B CN 109425573B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/031—Multipass arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
Abstract
The invention discloses a solution detection device and a detection method suitable for the detection device. Detection device uses with the test tube cooperation, detection device is provided with the detection box that can be used for installing test tube and chromatography test paper and is used for sealed upper cover and hou gai, the inside mounting groove that is provided with contact pin and drain hole of detection box realizes carrying out the effect that detects to the solution in the test tube under sealed environment, has saved and has used instruments such as burette, pipette to draw solution and has removed the process of chromatography test paper from the test tube. The invention provides a solution detection device with simple structure and high detection accuracy and a corresponding detection method.
Description
Technical Field
The invention relates to the field of solution detection, in particular to a solution detection device and a solution detection method.
Background
For many research, medical and industrial application fields, detection of solutions is often required, including volatile, polluted and toxic reagent solutions such as proteins and enzyme-linked immunosorbent assay, and in the detection process of the volatile solutions, the problems of environmental pollution such as volatile substances overflowing, falling or aerosol volatilization generated by large-area contact with the environment at room temperature need to be prevented.
For example, the PCR product solution is a solution in which one of the substances has a volatile substance. Polymerase Chain Reaction (Polymerase Chain Reaction), PCR for short, also called cell-free molecular cloning or specific DNA sequence in vitro primer directed enzymatic amplification technology, is a method for in vitro enzymatic synthesis of specific DNA fragments, and the obtained PCR product solution contains volatile components such as aerosol, so that the detection accuracy is prevented from being influenced by volatile substances in the detection process as much as possible.
In practical applications, in order to characterize the state of the solution, the solution needs to be tested, and usually a chromatographic test paper is used to test the solution.
For example, for the detection of a specific PCR product solution, except that the product overflows and falls off due to human misoperation, if the detection tube containing the PCR product solution to be detected is uncovered, the product solution contacts with the atmosphere, so that the volatilization of aerosol occurs, and the volatilization is inevitable. Generally, the concentration of the product after the PCR reaction is finished is very high, so that the product solution is very easy to pollute the environment under the condition of uncovering, the probability of false positive of the subsequent PCR detection is greatly improved, the accuracy of the subsequent detection result is influenced, and the polluted environment is very complicated and clean.
After amplification is performed using a conventional PCR reaction tube, if the end point of the product solution is required to be detected, the PCR product solution is usually extracted from the conventional PCR reaction tube by a dropper, a pipette or the like, and then dropped into a chromatographic test paper for detection. In the detection mode, the detection steps are complicated, external impurities can be mixed in the liquid transferring process of the PCR product, the precision of the detection result is influenced, and meanwhile, the problems of volatilization and pollution of aerosol cannot be effectively solved.
Similarly, for other volatile solutions, inaccurate detection results and environmental pollution caused by volatile substances in the detection process are problems to be solved urgently. Therefore, the detection device and the detection method which are simple and convenient to operate, can be used in both laboratory environments and field environments and can be effectively applied to volatile product solutions have important significance in rapid detection.
Disclosure of Invention
In order to overcome the problems in the related art, the invention provides a solution detection device and a detection method which are simple and convenient to operate and high in detection accuracy.
In order to achieve the purpose, the invention adopts the following technical scheme:
a solution detection device, the detection device 2 is used with a detection test tube 1, the detection test tube 1 comprises a tube body 11 and a cover 12, the outer wall of the lower end of the tube body 11 is a sealing film 13 which is coated, the detection device 2 comprises a detection box 21 and an upper cover 22 which are used for installing the detection test tube 1, the upper cover 22 is pivotally installed at the bottom of one end of the detection box 21 and can be buckled on the detection box 21, wherein,
the cartridge 21 is formed in a stepped shape, and includes a bottom plate 211, a sidewall 212 formed at one end of the bottom plate 211, and a top plate 213 formed at the top of the sidewall 212,
the top surface of one end of the top plate 213 adjacent to the upper cover 22 is provided with an installation groove 214 for installing the detection test tube 1, and the edge of the installation groove 214 is communicated with the edge of the detection box 21; a contact pin 2142 for piercing the sealing film 13 is disposed on the mounting groove bottom plate 2141, and a liquid guiding hole 2143 for communicating the mounting groove 214 and the chromatographic test paper 25 is disposed on the mounting groove bottom plate 2141 around the contact pin 2142;
an observation window 215 for observing the state change of the chromatography test paper 25 is further formed in the other end of the top surface of the top plate 213, and a test paper window 23 for sealing is mounted on the observation window 215;
a test strip stopper 216 for mounting a chromatography test strip 25 is provided on the back surface of the top plate 213, that is, inside the cassette 21;
an upper cover 22 is pivotally mounted at one end of the bottom plate 211, an opening 2111 for placing the chromatography test paper 25 is formed at the other end of the bottom plate, a rear cover 24 for sealing is configured, and the rear cover 24 is detachably mounted on the opening 2111.
Preferably, the mounting groove bottom plate 2141 of one end of the mounting groove 214 communicated with the edge of the detection box 21 is provided with a first limiting strip 2144, the connecting part of the side wall of the mounting groove 214 and the top plate 213 extends to the inside of the mounting groove 214 to be provided with a second limiting strip 2145, and a gap between the first limiting strip 2144 and the second limiting strip 2145 forms a socket limiting protrusion for inserting the detection test tube.
Preferably, the two sides of the middle part of the tube body 11 of the detection test tube 1 are provided with first limiting bulges 114,
the side wall of the mounting groove 214 is provided with a limiting groove 2148 matching with the first limiting protrusion 114 along a direction perpendicular to the mounting groove bottom plate 2141.
Preferably, a positioning column 115 is arranged at the end of the tube body 11 of the detection test tube 1;
the end of the mounting groove 214 is provided with a groove matched with the positioning column 115, the side walls of the two sides of the groove are respectively provided with a limiting column 2146, the positioning column 115 is mounted in the groove, and the side wall of the end of the tube body 11 is abutted to the limiting column 2146.
Preferably, one end of the contact pin 2142 connected to the mounting groove bottom plate 2141 is a cylindrical structure, and the other end is a tapered structure, and a liquid guiding groove 2147 for guiding the solution to be detected to flow to the liquid guiding hole 2143 is radially formed on the outer surface of the contact pin 2142.
Preferably, the chromatography test paper 25 may be a colloidal gold test paper, which includes a sample pad 251, a binding pad 252, a chromatography layer 253, and a water-absorbent material pad 254,
the reagent paper stopper 216 includes first supports 2161 provided at both ends of the rear surface of the top plate 213 and second supports 2162 provided at the middle portion,
the first support 2161 and the second support 2162 are positioned on the same straight line, the end where the sample pad 251 of the colloidal gold test paper is positioned is installed on the first support 2161 corresponding to one end of the mounting groove 214, the end where the water absorbing material pad 254 is positioned is installed on the other first support 2161, and the combination pad 252 is in contact with the second support 2162.
Preferably, two ends of the back surface of the top plate 213 are respectively provided with a limiting cylinder 2160, a side wall of each limiting cylinder 2160 is respectively provided with a notch to form the first support 2161, and two side edges of the notch are provided with second limiting protrusions 2163 for clamping the chromatography test paper 25;
a third limiting protrusion 2164 for limiting the initial installation position of the chromatography test paper 25 is disposed on the sidewall of the at least one limiting barrel 2160.
Preferably, the upper cover 22 includes a cover plate 220, a pressing ring 222 is disposed on an inner surface of the cover plate 220 and is matched with the insertion pin 2142, and an anti-slip pattern 227 is disposed on an outer surface of the cover plate 220;
the inner surface of the cover 220 is further provided with a first mounting post 223, the top plate 213 of the cartridge 21 is further provided with a first mounting hole 2131, and the first mounting post 223 is fittable into the first mounting hole 2131.
Preferably, the top plate 213 of the detection box 21 and two side edges of the plate surface at the end where the bottom plate 211 is connected to the upper cover 22 are respectively provided with a first sealing groove 217;
one end pivot of apron 220 is installed on detecting box 21, but the other end overlap joint is in on the roof 213, be provided with between the both ends can the lock be in the curb plate 221 of detecting box 21 both sides, the edge and the overlap joint of curb plate 221 are in the one end edge of roof 213 is provided with respectively and is used for sealed first sealing strip 224, first sealing strip 224 can the lock and the embedding is installed in first seal groove 217.
Preferably, at least one limit strip 226 is disposed on the side plate 221 of the upper cover 22 connected to the bottom plate 211, and the limit strip 226 can abut against the cover 12 of the test tube 1 installed in the installation groove 214.
Preferably, the observation window 215 is a groove formed in the top plate 213, and includes a window 2151 formed in a bottom plate of the groove, a text mark 2152 for representing a detection result, a sealing groove 2153 formed in a bottom edge of the groove, and a second mounting hole 2154 for mounting the test paper window 23;
the test paper window 23 is made of transparent plastic materials and comprises an observation sheet 230, wherein two ends of the inner surface of the observation sheet 230 are provided with second mounting columns 231, and the edge of the inner surface of the observation sheet 230 is provided with a second sealing strip 232 for sealing the observation window 215;
the second mounting post 231 of the viewing window 215 is mounted in the second mounting hole 2154, and the second sealing strip 232 is fittable within the sealing groove 2153.
Preferably, the inner surface of the rear cover 24 is provided with a plurality of third mounting posts 241, a third mounting hole 219 is provided on the back surface of the top plate 213 of the detection box 21, and the third mounting posts 241 can be inserted into the third mounting holes 219;
the two ends of the inner surface of the rear cover 24 are respectively provided with a fixing protrusion 243, and the fixing protrusions 243 can be abutted with the chromatography test paper 25 arranged in the detection box 21;
the edge of the rear cover 24 is further provided with a third sealing strip 242 for sealing, the edge of the opening 2111 of the base plate 211 is provided with a third sealing groove 2112, and the third sealing strip 242 can be embedded in the third sealing groove 2112.
Preferably, one end of the bottom plate 211 is provided with a hemispherical protrusion or a mounting hole, one end of the upper cover 22 is provided with a pivot shaft 228 matched with the hemispherical protrusion or the mounting hole, and the pivot shaft 228 is pivotally mounted at one end of the bottom plate 211.
In addition, the invention also provides a solution detection method, which comprises the following steps,
the solution to be tested is loaded into the test tube 1,
installing one end of the detection test tube 1 provided with the sealing film 13 in the installation groove 214;
the upper cover 22 is fastened with the detection box 21, the pressing ring 222 on the inner surface of the upper cover 22 presses the detection test tube 1, so that the inserting pin 2142 punctures the sealing film 13, and the solution to be detected flows into the sample pad 251 of the chromatography test paper 25 through the liquid guide hole 2143.
The invention provides a solution detection device and a corresponding detection method, wherein the detection device can be directly matched with a detection test tube filled with a solution to be detected for use, the detection device is provided with a detection box for installing the detection test tube and chromatographic test paper and an upper cover and a rear cover for sealing, the detection test tube filled with the solution to be detected can be directly inserted into the detection device, the upper cover is buckled and pressed, a contact pin and a liquid guide hole are arranged in an installation groove, the detection test tube is punctured through the matching of a pressing ring on the inner surface of the upper cover and the contact pin, the solution in the detection test tube is detected in a sealed environment, the process of extracting the solution from the test tube and moving the solution to the chromatographic test paper by using tools such as a dropper, a liquid moving gun and the like is omitted, and the operation mode is quick and convenient. Meanwhile, the detection device assembled by the detection box, the upper cover and the rear cover realizes a closed environment, can be applied to detection of solutions with volatile substances, effectively prevents the volatile substances from overflowing, avoids aerosol pollution, greatly reduces the probability of inaccurate detection results caused by aerosol pollution in subsequent experiments, and simultaneously avoids harm to operators and environmental pollution.
Drawings
In order to more clearly explain the technical solution of the present invention, the drawings needed to be used in the embodiments are briefly described below, and it is obvious for those skilled in the art to obtain other drawings without any inventive exercise.
FIG. 1 is a schematic overall view of an embodiment of the present invention with test tubes installed;
FIG. 2 is a schematic diagram of the overall structure of a test tube configured in accordance with an embodiment of the present invention;
FIG. 3 is a schematic view of the internal cross-section of a test tube configured in accordance with an embodiment of the present invention;
FIG. 4 is a schematic view of the overall structure of a detecting device according to an embodiment of the present invention;
FIG. 5 is a schematic cross-sectional view of a detecting device according to an embodiment of the present invention;
FIG. 6A is a schematic plan view of the top surface of a cartridge of the detecting unit in accordance with the embodiment of the present invention;
FIG. 6B is a schematic perspective view of the top surface of the cartridge of the detecting unit in accordance with one embodiment of the present invention;
FIG. 7 is a schematic view of a test device and test tube assembly according to one embodiment of the present invention;
FIG. 8 is a schematic plan view of a test tube mounted on a test device in accordance with an embodiment of the present invention;
FIG. 9 is a schematic view showing the attachment of the cartridge to the upper cover in one embodiment of the present invention;
FIG. 10A is a schematic view of the outer surface structure of the upper cover according to one embodiment of the present invention;
FIG. 10B is a schematic view of the inner surface of the upper cover according to one embodiment of the present invention;
FIG. 11 is a schematic view showing the engagement of the upper lid with the cartridge in one embodiment of the present invention;
FIG. 12A is a schematic plan view of the bottom surface of the cartridge of the detecting unit in accordance with the embodiment of the present invention;
FIG. 12B is a schematic perspective view of the bottom surface of the cartridge of the detecting unit in accordance with the embodiment of the present invention;
FIG. 13 is a schematic structural view of a chromatographic test strip in accordance with an embodiment of the present invention;
FIG. 14 is a schematic view showing the mounting of the chromatography test paper on the cartridge;
FIG. 15A is a schematic view of the bottom of a test strip window of the detecting device according to one embodiment of the present invention;
FIG. 15B is a schematic top view of a test strip window of the detecting device according to one embodiment of the present invention;
FIG. 16 is a schematic view of the test strip window mounted on the test cassette in accordance with one embodiment of the present invention;
FIG. 17 is a schematic view of the test strip window and the observation window being sealed together in accordance with an embodiment of the present invention;
FIG. 18 is a schematic view of the combination of the text label on the cartridge and the chromatographic strip in one embodiment of the present invention;
FIG. 19 is a schematic illustration of the inner surface configuration of the rear cover in one embodiment of the present invention;
FIG. 20 is a schematic view showing the mounting of the rear cover and the cartridge in one embodiment of the present invention;
FIG. 21 is a schematic view of the back cover and the cartridge in a mated seal in accordance with an embodiment of the present invention;
FIG. 22 is a schematic view of a three-position labyrinth seal in accordance with an embodiment of the present invention.
In the figure: 1-a detection test tube, 2-a detection device, 11-a tube body, 12-a cover, 13-a sealing film, 21-a detection box, 22-an upper cover, 23-a test paper window, 24-a rear cover, 25-chromatography test paper, 111-a sample injection port, 112-a sample injection channel, 113-a sample containing cavity, 114-a first limiting bulge, 115-a positioning column, 211-a bottom plate, 212-a side wall, 213-a top plate, 214-a mounting groove, 215-an observation window, 216-a test paper limiting piece, 217-a first sealing groove, 219-a third mounting hole, 220-a cover plate, 221-a side plate, 222-a pressure ring, 223-a first mounting column, 224-a first sealing strip, 225-a shallow groove, 226-a limiting strip and 227-an anti-skid texture, 228-a pivot shaft, 230-an observation plate, 231-a second mounting column, 232-a second sealing strip, 241-a third mounting column, 242-a third sealing strip, 243-a fixing protrusion, 251-a sample pad, 252-a bonding pad, 253-a chromatography layer, 254-a water absorbent material pad, 2111-an opening, 2112-a third sealing groove, 2131-a first mounting hole, 2141-a mounting groove bottom plate, 2142-a pin, 2143-a liquid guide hole, 2144-a first limit strip, 2145-a second limit strip, 2146-a limit column, 2147-a liquid guide groove, 2148-a limit groove, 2151-a window, 2152-a character mark, 2153-a sealing groove, 2154-a second mounting hole, 2160-a limit barrel, 2161-a first support, 2162-a second support, 2163-a second limit protrusion, 2164-third limit projection.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the present invention provides a solution detection device, where the solution may be a volatile solution or a non-volatile solution, and this embodiment is mainly illustrated by a volatile PCR product solution, and of course, the present invention may also be applied to a volatile, contaminated, and toxic and harmful reagent solution including protein, enzyme-linked immunoassay, and the like, and specific embodiments may be adapted to different solutions and chromatography test strips, and are not limited herein.
Referring to the embodiment shown in fig. 1 to 3, the detection device 2 is used in cooperation with a detection test tube 1, the detection test tube 1 includes a tube 11 and a cover 12, and the outer wall of the lower end of the tube 11 is a sealing film 13 disposed in an enclosing manner. One end of the tube body 11 corresponding to the cover 12 is provided with a sample injection port 111, the other end is provided with a sample cavity 113, and the sample injection port 111 is communicated with the sample cavity 113 through a sample injection channel 112.
Referring to fig. 4 to 8, the detecting device 2 includes a detecting box 21 for mounting the detecting test tube 1, an upper cover 22, the upper cover 22 is pivotally mounted at the bottom of one end of the detecting box 21 and can be buckled on the detecting box 21, wherein the detecting box 21 is of a ladder type and includes a bottom plate 211, a side wall 212 arranged at one end of the bottom plate 211, and a top plate 213 arranged at the top of the side wall 212.
The top surface of one end of the top plate 213 adjacent to the upper cover 22 is provided with an installation groove 214 for installing the detection test tube 1, and the edge of the installation groove 214 is communicated with the edge of the detection box 21; a contact pin 2142 for piercing the sealing film 13 is disposed on the mounting groove bottom plate 2141, and a liquid guiding hole 2143 for communicating the mounting groove 214 and the chromatographic test paper 25 is disposed on the mounting groove bottom plate 2141 around the contact pin 2142. After the plug pin 2142 punctures the sealing film 13 of the test tube 1, the PCR product solution to be detected flows out from the punctured part, and flows onto the chromatography test paper 25 mounted on the test paper limiting member 216 along the liquid guiding hole 2143 for detection, and a detection result is obtained.
Preferably, one end of the contact pin 2142 connected to the mounting groove bottom plate 2141 is a cylindrical structure, the other end of the contact pin 2142 is a tapered structure, a liquid guiding groove 2147 for guiding the solution to be detected to flow to the liquid guiding hole 2143 is radially formed in the outer surface of the contact pin 2142, and after the sealing film 13 is punctured, the solution to be detected can conveniently flow into the liquid guiding hole 2143 through the liquid guiding groove 2147 and then flow onto the chromatography test paper 25 for detection.
An observation window 215 for observing the state change of the chromatography test paper 25 is further formed in the other end of the top surface of the top plate 213, and a test paper window 23 for sealing is mounted on the observation window 215.
A test strip stopper 216 for mounting a chromatography test strip 25 is provided on the back surface of the top plate 213, that is, inside the cassette 21; an upper cover 22 is pivotally mounted at one end of the bottom plate 211, an opening 2111 for placing the chromatography test paper 25 is formed at the other end of the bottom plate, a rear cover 24 for sealing is configured, and the rear cover 24 is detachably mounted on the opening 2111.
The embodiment provides a convenient solution detection device, for avoiding secondary pollution, detection device 2 is disposable form, detection device 2 uses with inside detection test tube 1 cooperation that is equipped with the solution that detects, for example, can directly install detection test tube 1 that is equipped with the PCR product solution after the amplification on detection device 2 and detect, need not to open detection test tube 1 and take a sample, avoided because of opening the influence of reasons such as the volatilization that the lid caused or connect the department with the air to the testing result accuracy to can convenient and fast detect solution.
Referring to fig. 6A to 8, in order to ensure that the test tube 1 is installed at a predetermined position, the test box 21 is further provided with a limiting device. Wherein, mounting groove bottom plate 2141 of mounting groove 214 and the edge of detection box 21 one end that is linked together is provided with first spacing 2144, and the department of being connected of lateral wall and roof 213 is provided with the spacing 2145 of second to the inside extension of mounting groove 214, the clearance of first spacing 2144 and the spacing 2145 of second forms the socket that is used for inserting the detection test tube, detection test tube 1 passes through socket detachable installs on the detection box 21. Of course, the mounting groove 214 may also be provided with other structures for mounting the test tube 1, and is not limited herein.
Preferably, the two sides of the middle part of the tube body 11 of the detection test tube 1 are provided with first limiting protrusions 114, and a limiting groove 2148 matched with the first limiting protrusions 114 is formed in the side wall of the mounting groove 214 along the direction perpendicular to the mounting groove bottom plate 2141. The first limiting protrusion 114 can be clamped in the limiting groove 2148 during installation, and the stability of the installation of the detection test tube 1 is further increased.
In addition, the tip of body 11 of test tube 1 is provided with reference column 115, the tip of mounting groove 214 is provided with the recess that mutually supports with reference column 115, be provided with spacing post 2146 on the lateral wall of recess both sides respectively, reference column 115 installs in the recess and the lateral wall of body 11 tip with spacing post 2146 looks butt.
Referring to fig. 9, which is a schematic view of the installation of the upper cover 22 and the detection box 21, referring to fig. 10A and 10B, the upper cover 22 includes a cover plate 220, and a pressing ring 222 is disposed on an inner surface of the cover plate 220 and is engaged with the insertion pin 2142, as shown in the oval mark of fig. 11, the pressing ring 222 presses an end of the detection tube 1 where the sealing film 13 is disposed, so that the sealing film 13 deforms, and then contacts with the insertion pin 2142 and is punctured by the insertion pin 2142.
The inner surface of the cover 220 is further provided with a first mounting post 223, the top plate 213 of the cartridge 21 is provided with a first mounting hole 2131, and the first mounting post 223 is fittable into the first mounting hole 2131. Meanwhile, the top plate 213 of the detecting box 21 and two side edges of one end of the bottom plate 211 connected to the upper cover 22 are respectively provided with a first sealing groove 217.
Be provided with anti-skidding line 227 on the surface of apron 220, friction when anti-skidding line 227 can increase to press improves staff's operating stability.
One end of the cover plate 220 is pivotally mounted on the detection box 21, the other end of the cover plate can be lapped on the top plate 213, side plates 221 which can be buckled on two sides of the detection box 21 are arranged between two ends of the cover plate, the edges of the side plates 221 and the edges of one end of the top plate 213 are respectively provided with a first sealing strip 224 for sealing, the first sealing strips 224 can be buckled and embedded in the first sealing grooves 217, namely, the sealing between the upper cover 22 and the detection box 21 is realized through a labyrinth sealing mode, the schematic diagram of the labyrinth sealing is shown in a circular mark of fig. 11, and the labyrinth sealing mode is adopted, so that the whole detection box can prevent the solution after the test tube is punctured from contacting with the outside air under normal pressure, and the pollution of volatile substances such as aerosol is generated.
In addition, at least one limit strip 226 is disposed on the side plate 221 of the upper cover 22 connected to the bottom plate 211, and the limit strip 226 can abut against the cover 12 of the test tube 1 installed in the installation groove 214. When the lock upper cover 22, spacing 226 can support the lid 12 tip that detects test tube 1 to prevent detection test tube 1 from withdrawing from the mounting groove 214 when using, realize spacing effect.
In addition, the side plate 221 is provided with a shallow groove 225 on the outer surface thereof, the shallow groove 225 provides a force bearing point, and if the upper cover 22 and the detection box 21 are required to be separated, a worker can open the upper cover 22 and the detection box 21 by applying force through the shallow groove 225 to take out the detection test tube 1.
When the test tube 1 is installed in the mounting groove 214, the upper cover 22 is buckled, the pressing ring 222 presses the one end where the sealing film 13 of the test tube 1 is located, and makes it crooked, the sealing film 13 on the test tube 1 is punctured to the contact pin 2142, and then makes the PCR product solution flow out of the test tube 1 and flow to the chromatography test paper 25 through the drain hole 2143. The first mounting column 223 is matched with the first mounting hole 2131, so that the upper cover 22 can not be separated, and the stability of the detection process is ensured.
The chromatographic test paper 25 is mounted inside the cassette 21, and the test paper stopper 216 is provided on the back surface of the top plate 213. Specifically, as shown in fig. 12A and 12B, in an embodiment of the test strip stopper 216, two ends of the back surface are respectively provided with a stopper barrel 2160, a side wall of each stopper barrel 2160 is respectively provided with a notch and forms the first support 2161, two side edges of the notch are provided with a second stopper protrusion 2163 for clamping the chromatography test strip 25, and a side wall of at least one stopper barrel 2160 is provided with a third stopper protrusion 2164 for limiting an initial installation position of the chromatography test strip 25. The second support 2162 is a support beam disposed between the mounting slot 214 and the observation window 215.
The limiting cylinder 2160 may be configured to be a cylinder or a square column, and the limiting cylinder 2160 of the embodiment shown in fig. 12B is a square column, and is adaptively adjusted according to the structure of the mounting groove 214, so as to ensure that two ends of the chromatography test paper 25 can be stably mounted on the two limiting cylinders. Of course, the test strip stopper 216 may have other structures than the stopper barrel 2160 to mount the chromatographic test strip 25 inside the cassette 21.
As shown in FIG. 13, one embodiment of the chromatographic test strip 25 used for detection is a colloidal gold test strip used for detection of a PCR product solution in this embodiment, which is a wide variety of chromatographic test strips 25. The colloidal gold test paper is used as one of the chromatographic test papers 25, and comprises a sample pad 251, a combination pad 252, a chromatographic layer 253 and a water absorbing material pad 254 which are sequentially arranged, the test paper limiting member 216 comprises a first support 2161 and a second support 2162, the first support 2161 and the second support 2162 are arranged at two ends of the back of the top plate 213, the first support 2161 and the second support 2162 are positioned on the same straight line, one end of the colloidal gold test paper where the sample pad 251 is positioned is arranged on the first support 2161 corresponding to one end of the mounting groove 214, one end of the water absorbing material pad 254 is arranged on the other first support 2161, and the combination pad 252 is in contact with the second support 2162. In addition, other corresponding chromatography test paper 25 may be used for detecting other volatile solutions, and is not limited herein.
Fig. 14 is a schematic view illustrating the installation of the chromatography test paper 25 and the detection box 21, in particular, when the chromatography test paper 25 is installed, one end or both ends of the chromatography test paper 25 are limited by the third limiting protrusion 2164 to an initial position of installation, wherein the sample pad 251 of the chromatography test paper 25 is disposed below the liquid guide hole 2143, the binding pad 252 is in contact with the second support 2162, and the chromatography layer 253 is disposed opposite to the observation window 215. The solution to be detected flows to the sample pad 251 through the liquid guide hole 2143, flows to the middle chromatography layer 253 under the action of the absorbent material pad 254 at the other end of the chromatography test paper 25, and shows the detection result on the chromatography layer 253.
Referring to fig. 6A and 6B, the observation window 215 is a groove formed in the top plate 213, and includes a window 2151 formed in a bottom plate of the groove, a text mark 2152 for representing a detection result, a sealing groove 2153 formed in a bottom edge of the groove, and a second mounting hole 2154 for mounting the test paper window 23.
Referring to fig. 15A and 15B, the test paper window 23 is made of a transparent plastic material, and includes an observation sheet 230, wherein second mounting posts 231 are disposed at two ends of an inner surface of the observation sheet 230, and a second sealing strip 232 for sealing the observation window 215 is disposed at an edge of the inner surface of the observation sheet 230; the second mounting post 231 of the observation window 215 is mounted in the second mounting hole 2154, and the second sealing strip 232 can be embedded in the sealing groove 2153, and fig. 16 is a schematic view illustrating the mounting of the test paper window 23. The construction still employs the principles of labyrinth sealing, as shown in particular in fig. 17. The test paper window 23 can clearly observe the detection result and can seal and protect the observation window 215.
Meanwhile, since the detected targets are different and the matched chromatographic test strips 25 are different, the detected result is represented by the character marks 2152, referring to fig. 18, the display lines of the chromatographic layer 253 correspond to the character marks 2152, that is, C, T lines or C, T1, T2 and the like, one to one, that is, different detected results correspond to different character marks 2152, respectively, and the operator can judge the detected result through the observation window 215 and the test strip window 23.
Meanwhile, in order to secure the mounting stability of the chromatography test paper 25, referring to fig. 19, the inner surface of the rear cover 24 is provided with a plurality of third mounting posts 241, and a third mounting hole 219 is provided on the back surface of the top plate 213 of the cartridge 21, and the third mounting posts 241 can be fitted into the third mounting holes 219. The two ends of the inner surface of the rear cover 24 are respectively provided with a fixing protrusion 243, and the fixing protrusions 243 can be abutted against the chromatography test paper 25 installed in the detection box 21. By matching with the first support 2161 and the second support 2162, the chromatographic test paper 25 can be stably installed in the detection box 21, and the detection process is not affected by the movement of the chromatographic test paper 25 in the detection process. FIG. 20 is a schematic view showing the mounting of the rear cover and the cartridge 21.
In addition, referring to fig. 12A and 12B and fig. 15A, a labyrinth seal structure is also disposed between the bottom plate 211 and the rear cover 24, specifically, the labyrinth seal structure includes a third seal strip 242 disposed at an edge of the rear cover 24, and a third seal groove 2112 is disposed at an edge of an opening 2111 of the bottom plate 211, and the third seal strip 242 is capable of being embedded in the third seal groove 2112. As shown in FIG. 21, the labyrinth seal structure can effectively seal the bottom of the cartridge 21 and prevent the excess PCR product solution from overflowing from the bottom of the cartridge 21.
By combining the three sealing structures, as shown in fig. 22, the sealing of the detection device can be effectively realized, the solution is prevented from overflowing when the sealing film 13 is punctured in the detection process, the harm of the solution to the operator and the pollution to the environment are prevented, and meanwhile, the inaccurate or unstable detection result caused by the change of the content of volatile substances can be avoided.
The application also comprises a solution detection method, wherein the detection method is realized by adopting the detection device, and concretely, the detection method comprises the following steps.
Filling a solution to be detected in a detection test tube 1;
installing one end of the detection test tube 1 provided with the sealing film 13 in the installation groove 214;
the upper cover 22 is buckled with the detection box 21, so that the contact pin 2142 punctures the sealing film 13, and the solution to be detected flows into the sample pad 251 of the chromatographic test paper 25 through the liquid guide hole 2143;
the detection result is judged through the observation window 215. The remaining detection steps consistent with conventional detection methods are not overly limited or illustrated herein.
Taking a PCR product solution as a specific example for explanation, a reagent solution is loaded in the detection test tube 1, and a specific nucleic acid fragment in the reagent solution is proliferated, and the proliferated solution is a PCR product solution to be detected. During detection, the detection test tube 1 filled with the PCR solution can be directly installed in the detection device for detection, the step of opening the detection test tube 1 for sampling is omitted, meanwhile, the condition that the detection result of the PCR product solution is inaccurate at the joint of the opened cover 12 and air can be avoided, the operation steps are simplified, and the detection accuracy is also improved.
The embodiment of the invention provides a solution detection device and a corresponding detection method, wherein the detection device can be directly matched with a detection test tube filled with a solution to be detected for use, the detection device is provided with a detection box for installing the detection test tube and chromatography test paper, and an upper cover and a rear cover for sealing, the detection test tube filled with the solution to be detected can be directly inserted into the detection device, the upper cover is buckled and pressed, the inside of the detection box is provided with an installation groove of a contact pin and a liquid guide hole, the detection of the solution in the detection test tube in a sealed environment is realized, the process of extracting the solution from the test tube and moving the solution to the chromatography test paper by using tools such as a dropper, a liquid transfer gun and the like is omitted, and the operation mode is quick and convenient. Meanwhile, the detection device assembled by the detection box, the upper cover and the rear cover realizes a closed environment, is particularly suitable for detection of solution with volatile substances besides general solution, can effectively prevent the volatile substances from overflowing, avoids aerosol pollution, greatly reduces the probability of inaccurate detection result caused by aerosol pollution in subsequent experiments, and simultaneously avoids causing harm to operators and environmental pollution.
Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It is intended that the specification and examples be considered as exemplary only, with a scope of the invention being limited only by the following claims.
Claims (14)
1. A solution detection device, characterized in that: the detection device (2) is matched with the detection test tube (1) for use, the detection test tube (1) comprises a tube body (11) and a cover (12), the outer wall of the lower end of the tube body (11) is a sealing film (13) which is coated, the detection device (2) comprises a detection box (21) and an upper cover (22) which are used for installing the detection test tube (1), the upper cover (22) is pivotally installed at the bottom of one end of the detection box (21) and can be buckled on the detection box (21), wherein,
the detection box (21) is arranged in a ladder shape and comprises a bottom plate (211), a side wall (212) arranged at one end of the bottom plate (211) and a top plate (213) arranged at the top of the side wall (212),
the top surface of one end of the top plate (213), which is adjacent to the upper cover (22), is provided with a mounting groove (214) for mounting the detection test tube (1), and the edge of the mounting groove (214) is communicated with the edge of the detection box (21); a contact pin (2142) for puncturing the sealing film (13) is arranged on the mounting groove bottom plate (2141), and a liquid guide hole (2143) for communicating the mounting groove (214) and the chromatography test paper (25) is formed in the mounting groove bottom plate (2141) on the peripheral side of the contact pin (2142);
an observation window (215) used for observing the state change of the chromatographic test paper (25) is further formed in the other end of the top surface of the top plate (213), and a test paper window (23) used for sealing is mounted on the observation window (215);
a test strip stopper (216) for mounting a chromatography test strip (25) is arranged on the back surface of the top plate (213), namely inside the detection box (21);
an upper cover (22) is pivotally mounted at one end of the bottom plate (211), an opening (2111) for placing the chromatography test paper (25) is formed in the other end of the bottom plate, a rear cover (24) for sealing is configured, and the rear cover (24) is detachably mounted on the opening (2111).
2. The solution detecting apparatus according to claim 1, characterized in that:
mounting groove bottom plate (2141) of mounting groove (214) and detection box (21) edge one end of being linked together is provided with first spacing (2144), and the lateral wall of mounting groove (214) is provided with second spacing (2145) to the inside extension of mounting groove (214) with the department of being connected of roof (213), the clearance of first spacing (2144) and second spacing (2145) forms the socket that is used for inserting the detection test tube.
3. The solution detecting apparatus according to claim 2, characterized in that:
two sides of the middle part of the tube body (11) of the detection test tube (1) are provided with first limiting bulges (114),
and a limiting groove (2148) matched with the first limiting bulge (114) is formed in the side wall of the mounting groove (214) along the direction vertical to the mounting groove bottom plate (2141).
4. The solution detecting apparatus according to claim 1 or 2, characterized in that:
a positioning column (115) is arranged at the end part of the tube body (11) of the detection test tube (1);
the end of the mounting groove (214) is provided with a groove matched with the positioning column (115), the side walls of two sides of the groove are respectively provided with a limiting column (2146), the positioning column (115) is mounted in the groove, and the side wall of the end of the tube body (11) is abutted to the limiting column (2146).
5. The solution detecting apparatus according to claim 1 or 2, characterized in that:
the liquid guide device is characterized in that one end, connected with the mounting groove bottom plate (2141), of the contact pin (2142) is of a cylindrical structure, the other end of the contact pin is of a conical structure, and a liquid guide groove (2147) used for guiding a solution to be detected to flow to the liquid guide hole (2143) is formed in the outer surface of the contact pin (2142) along the radial direction of the cylinder.
6. The solution detecting apparatus according to claim 1, characterized in that:
the chromatography test paper (25) can be colloidal gold test paper, the colloidal gold test paper comprises a sample pad (251), a combination pad (252), a chromatography layer (253) and a water-absorbing material pad (254) which are arranged in sequence,
the test paper stopper (216) comprises a first support (2161) arranged at two ends of the back of the top plate (213) and a second support (2162) arranged in the middle,
the first support (2161) and the second support (2162) are positioned on the same straight line, one end of the sample pad (251) of the colloidal gold test paper is arranged on the first support (2161) corresponding to one end of the mounting groove (214), one end of the water absorbing material pad (254) is arranged on the other first support (2161), and the combination pad (252) is in contact with the second support (2162).
7. The solution detecting apparatus according to claim 6, characterized in that:
two ends of the back of the top plate (213) are respectively provided with a limiting cylinder (2160), the side wall of each limiting cylinder (2160) is respectively provided with a notch to form the first support (2161), and the edges of two sides of the notch are provided with second limiting bulges (2163) for clamping the chromatography test paper (25);
the side wall of at least one limiting barrel (2160) is provided with a third limiting bulge (2164) used for limiting the initial installation position of the chromatography test paper (25).
8. The solution detecting apparatus according to claim 1, characterized in that:
the upper cover (22) comprises a cover plate (220), a pressing ring (222) matched with the inserting pin (2142) is arranged on the inner surface of the cover plate (220), and anti-skid grains (227) are arranged on the outer surface of the cover plate (220);
the inner surface of the cover plate (220) is also provided with a first mounting column (223), the top plate (213) of the detection box (21) is also provided with a first mounting hole (2131), and the first mounting column (223) can be embedded in the first mounting hole (2131).
9. The solution detecting apparatus according to claim 8, characterized in that:
first sealing grooves (217) are respectively formed in the top plate (213) of the detection box (21) and the edges of two sides of the plate surface at the end, connected with the upper cover (22), of the bottom plate (211);
one end pivot of apron (220) is installed on detecting box (21), but the other end overlap joint is in on roof (213), be provided with between the both ends can the lock detect curb plate (221) of box (21) both sides, the edge and the overlap joint of curb plate (221) are in the one end edge of roof (213) is provided with respectively and is used for sealed first sealing strip (224), first sealing strip (224) can lock and the embedding is installed in first seal groove (217).
10. The solution detecting apparatus according to claim 9, characterized in that:
at least one limit strip (226) is arranged on the side plate (221) connected with the bottom plate (211) of the upper cover (22), and the limit strip (226) can be abutted to the cover (12) of the detection test tube (1) installed in the installation groove (214).
11. The solution detecting apparatus according to claim 1, characterized in that:
the observation window (215) is a groove arranged on the top plate (213) and comprises a window (2151) arranged on a bottom plate of the groove, a character mark (2152) used for representing a detection result, a sealing groove (2153) arranged on the edge of the bottom of the groove and a second mounting hole (2154) used for mounting a test paper window (23);
the test paper window (23) is made of transparent plastic materials and comprises an observation sheet (230), second mounting columns (231) are arranged at two ends of the inner surface of the observation sheet (230), and a second sealing strip (232) used for sealing the observation window (215) is arranged at the edge of the inner surface of the observation sheet (230);
a second mounting post (231) of the viewing window (215) is mounted in the second mounting hole (2154), and the second sealing strip (232) is embeddable mounted within the sealing groove (2153).
12. The solution detecting apparatus according to claim 1, characterized in that:
the inner surface of the rear cover (24) is provided with a plurality of third mounting columns (241), the back surface of the top plate (213) of the detection box (21) is provided with third mounting holes (219), and the third mounting columns (241) can be embedded and mounted in the third mounting holes (219);
the two ends of the inner surface of the rear cover (24) are respectively provided with a fixing bulge (243), and the fixing bulges (243) can be abutted with chromatography test paper (25) arranged in the detection box (21);
the edge of back lid (24) still is provided with third sealing strip (242) that are used for sealed, the edge of opening (2111) of bottom plate (211) is provided with third seal groove (2112), third sealing strip (242) can the embedding install in third seal groove (2112).
13. The solution detecting apparatus according to claim 1, characterized in that:
one end of the bottom plate (211) is provided with a hemispherical protrusion or a mounting hole, one end of the upper cover (22) is provided with a pivot shaft (228) matched with the hemispherical protrusion or the mounting hole, and the pivot shaft (228) is pivotally mounted at one end of the bottom plate (211).
14. A method for performing solution testing using the solution testing device according to any one of claims 1 to 13, comprising,
the solution to be detected is filled in a detection test tube (1),
installing one end, provided with a sealing film (13), of the detection test tube (1) in an installation groove (214);
the upper cover (22) is buckled with the detection box (21), the pressing ring (222) on the inner surface of the upper cover (22) presses the detection test tube (1), so that the inserting needle (2142) punctures the sealing film (13), and the solution to be detected flows into the sample pad (251) of the chromatographic test paper (25) through the liquid guide hole (2143).
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| CN201710770834.4A CN109425573B (en) | 2017-08-31 | 2017-08-31 | Solution detection device and detection method |
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| CN201710770834.4A CN109425573B (en) | 2017-08-31 | 2017-08-31 | Solution detection device and detection method |
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| CN111272744B (en) * | 2020-01-21 | 2022-03-11 | 北京理工大学珠海学院 | Detection apparatus for antibiotic in detection cow's milk |
| CN111965175A (en) * | 2020-08-12 | 2020-11-20 | 武汉普利赛斯科技有限公司 | Preparation method of AIDS blood test paper |
| CN113075396A (en) * | 2021-03-25 | 2021-07-06 | 江苏硕世生物科技股份有限公司 | Detection auxiliary device |
| CN117129671B (en) * | 2023-10-26 | 2024-01-23 | 苏州星安生物科技有限公司 | Excrement sample collection and detection integrated device |
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Address after: 100176 floor 01-5, building 16, yard 25, Jinghai 4th Road, Beijing Economic and Technological Development Zone, Daxing District, Beijing Patentee after: Beijing jinnuomi Technology Co.,Ltd. Address before: 100176 room 407, building 2, No. 19, Yongchang South Road, Yizhuang Economic Development Zone, Daxing District, Beijing Patentee before: BEIJING GENOME BIOTECHNOLOGY CO.,LTD. |