JP2016188184A - Production method of bee products extract, and bee products extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To naturally exhaustively obtain extracts abundantly containing the content component of bee products, such as bee pollen and propolis, in a form that can be absorbed and used effectively in a body such as a human body.SOLUTION: Bee products are frozen with liquefied gas and milled; water is added to the obtained milled products and a suspension is obtained; and the suspension is treated by a supervoltage homogenizer and the content component of the bee products is extracted. Preferably, from the treated solution obtained in the extraction, coarse particles are removed, cyclodextrin is mixed, and dry pulverization is performed.SELECTED DRAWING: None

Description

  The present invention relates to a method for producing a bee product extract using bee products such as pollen and propolis, and a bee product extract produced by the method.

  The bees collect pollen from their bodies when they visit the flowers in a pollen basket (pollen basket) in the hind legs, mix them with the nectar, take them into dumplings, take them back to the nest, and store them as food for themselves and their offspring. This pollen dumpling is called pollen load and contains a balanced amount of nutrients such as protein, carbohydrates, lipids, minerals and vitamins, and is the main nutrient source for bees. In addition, the bee is digested and metabolized to become the royal jelly, the staple food of the queen bee.

  Propolis is a resinous natural product, also called bee ani, applied to the nests and gaps in the nest, consisting of sap, plant essential oil, plant fibers, bee saliva, beeswax, etc. collected by the bees outdoors. Has been. It is thought to play a role such as reinforcing the nest structurally, preventing decay in the nest, and protecting the nest from external enemies such as microorganisms and insects. Propolis also has reports on various physiological activities such as anti-aging, anti-obesity, anti-allergy, and anti-cold.

  Conventionally, nutritional supplements, health foods, feeds, and the like in which the above-mentioned bee products are ingested by humans and animals are known. However, pollen has a hard outer membrane, and propolis is also a resinous natural product, and it has been difficult to prepare so that the content components contained therein can be used effectively.

  Regarding such a problem, for example, Patent Document 1 discloses a royal jelly-like fermented pollen having an improved digestion and absorption rate produced by fermenting purified pollen produced by crushing and refining the shell wall of nectar pollen. Nutritional preparations and powdered fermented pollen nutritional preparations obtained by drying them are described.

  Patent Document 2 discloses a propolis composition containing a hydrophilic organic solvent extraction propolis, a water extraction propolis, and a supercritical extraction propolis, wherein the hydrophilic organic solvent is added to 1 part by weight of the supercritical extraction propolis. A propolis composition containing 1-20 parts by weight of solvent-extracted propolis and 0.5-6 parts by weight of water-extracted propolis is described.

  In Patent Document 3, the pollen grains are immersed in a low-temperature liquefied gas and frozen to freeze, and in this state, the pollen grains are crushed by an impact pulverizer and the contents are extracted. Is described.

  Patent Document 4 discloses that a flow of pollen and liquid flows into an ultrafine atomization device under high pressure, and the pollen shell is pulverized by the device to form a fine pollen component and liquid. It is described that it is allowed to flow out as a fluid, and liquid is removed from the fluid and taken out as a pollen component.

  In Patent Document 5, fine pollen particles obtained by washing, pulverizing, and sterilizing a bee pollen load are added and mixed in electrolytic reduced water having a reducing property and containing active hydrogen. Then, the mixture is adjusted to form a fluid, and the fluid is pumped to an ultrafine atomizer, and active oxygen generated during the ultrafine particle process by active hydrogen in the electrolytic reduced water is reduced. It is described that the pollen component is extracted from the ultrafinely divided pollen particles by erasing and making the entire pollen ultrafine and crushing the shell without deteriorating the pollen component.

  Further, Patent Document 6 discloses that a powder of propolis powder, tap water, or natural water obtained by electrolysis is not containing chlorine, is alkaline with a pH of about 8 to 10, and has a redox potential of 200 mV or less. In addition, by introducing a fluid mixture of electroreduction water having characteristics including active hydrogen into an ultrafine powdering device, the action of the electroreduction water does not degrade the active ingredients of propolis, It is described that the active ingredient of the propolis in the propolis powder is extracted into the electrolytic reduced water, and the fluid is used as it is as a beverage.

JP-A-5-30926 JP 2003-61593 A JP 59-146574 A JP 2001-119 A JP 2007-37529 A JP 2007-244316 A

  However, in the method of fermenting pollen using koji molds, yeasts, lactic acid bacteria, vitamins and the like as in Patent Document 1, the content components of the bee products are altered by fermentation and cannot be extracted as they are.

  Moreover, in the method of combining hydrophilic organic solvent extraction, water extraction, and supercritical extraction as in Patent Document 2, it is difficult to extract the whole content components of bee products. Moreover, the removal of the organic solvent and the work of different extraction processes are complicated.

  In addition, it is possible to perform effective pulverization even if it is a resinous material such as pollen or propolis having a hard outer film by impact pulverization at extremely low temperatures as in Patent Document 3. Only by the pulverization by such a method, it is difficult to say that the content components of the bee products remain contained in the pulverized pieces and are effectively absorbed and used by the human body.

  In addition, as in Patent Documents 4 to 6, it is possible to perform crushing and extraction together by impact pulverization under high pressure while suspended in a solvent such as water, but depending on the energy for crushing. It was an inevitable method of deterioration and deterioration such as decomposition of the contents of bee products.

  Therefore, an object of the present invention is to obtain an extract containing abundantly the content components of bee products such as pollen cargo and propolis in a form that is effectively absorbed and utilized in the body of humans and the like. .

  In order to solve the above problems, the present invention includes a freeze pulverization step of freezing and pulverizing bee products with a liquefied gas, a hydrolyzing step of adding water to the obtained pulverized product to obtain a suspension, and an obtained suspension. And an extraction step of extracting the content component of the bee product by treating the suspension with an ultra-high pressure homogenizer.

  According to the present invention, a bee product is frozen with a liquefied gas and pulverized, thereby suppressing deterioration and deterioration of the content components of the bee product due to oxidation or the like, for example, a resin such as pollen or propolis having a hard outer film. Even if it is a shape, it can be effectively crushed. Next, water is added to the crushed material to form a suspension, and the suspension is treated with an ultra-high pressure homogenizer, for example, to promote separation of the pollen outer membrane and its contents, or to finely homogenize the propolis components. The content component of bee products can be effectively absorbed and utilized in the body of humans and the like, and the extract can be obtained in an abundant manner by extracting it in its natural form.

  The method for producing a bee product extract of the present invention preferably further includes a pulverization step of removing coarse particles from the treatment liquid obtained in the extraction step, mixing with cyclodextrin, and forming a dry powder. According to this, the component extracted in the extraction process is included in cyclodextrin and pulverized, thereby suppressing deterioration and deterioration of the component and improving the efficiency of absorption and use in the human body. It can be further improved. Moreover, the powder extract of the form which is easy to process into a tablet, a capsule, etc. can be provided.

  In addition, the water addition step is performed by adding drinking water to the ion exchange resin to remove minerals, then bringing it into contact with obsidian to form reduced water, and further adding active reduced water obtained by contacting with tourmaline. It is preferable. According to this, the process after a hydration process can be performed, suppressing the quality change and deterioration of the content component of bee products by the high reducibility of the active reduction water. Moreover, since the active reduced water is excellent also in the surface activity-like characteristic, the efficiency of extracting the content component of the bee product can be further improved.

  In the extraction step, the suspension is preferably treated with an ultrahigh pressure homogenizer having a pressure of 2000 atmospheres or more.

  Moreover, it is preferable that the said bee products are pollen load and / or propolis.

  On the other hand, another aspect of the present invention provides a bee product extract produced by the above production method.

  ADVANTAGE OF THE INVENTION According to this invention, the extract which contains abundantly the content component of bee products, such as pollen load and a propolis, in the form effectively absorbed and utilized by human bodies etc. without leaving a natural remains.

It is process drawing of one Embodiment of the manufacturing method of the bee product extract of this invention. It is process drawing of other embodiment of the manufacturing method of the bee product extract of this invention.

  Examples of the bee product to which the present invention is applied include pollen cargo, propolis, and bees. In particular, it is preferably applied to pollen cargo and propolis, which have conventionally been difficult to produce extracts. The pollen load was equipped with a filter with holes through which the worker bees must pass using their legs at the nest gate of the beehive, and the pollen load that the worker bees returned to the nest dropped on their legs was prepared at the bottom. It can be collected by collecting in a saucer. When propolis is scraped off the natural formation in a beehive box or sprayed with a barb ingredient that bees don't like on the board, the honeycomb will be propolis on the board to keep the nest clean. It can be collected by scraping it off.

  Hereinafter, the method for producing the bee product extract of the present invention will be specifically described with reference to the process diagram of FIG.

(1) Freezing and grinding step (S1)
The raw material can be appropriately coarsely crushed, adjusted in water content, or tested for the number of general viable bacteria, if necessary. The size of coarse pulverization varies depending on the apparatus used, but is typically about 0.1 to 5 mm square, more typically about 1 to 3 mm square. . Conventionally, an apparatus well known to those skilled in the art can be used for freeze grinding. A typical configuration is described as follows: a liquefied gas supply tank, a raw material hopper, a raw material cooler, a pulverizer, and a sieve are provided. The raw material hopper raw material is charged and the raw material is supplied to the raw material cooler. The product temperature is about −180 ° C. to −50 ° C., more typically −130 ° C. to −50 by being immersed, contacted, or indirectly contacted with the liquefied gas supplied from the liquefied gas supply tank. It becomes frozen at about ℃. Examples of the liquefied gas include liquid nitrogen (boiling point: -196 ° C). And if it grind | pulverizes with a grinder, maintaining the state, since the raw material has become low-temperature embrittlement, even if it is a resinous material such as pollen or propolis having a hard outer film, it can be effectively crushed. . The pulverization method is not particularly limited, and for example, an airflow pulverizer such as a jet mill or an impact pulverizer such as a power mill can be used. After pulverization, a sieve having a predetermined particle size is selected by sieving as necessary and used in the subsequent steps. In addition, what exceeds a predetermined particle size may be returned to the raw material hopper or the raw material cooler again and reused as the raw material. The particle size of the pulverized product should be such that 85% by mass or more of the total particle size falls within the range of 1 to 100 μm (for example, a sieve having a mesh opening of 100 μm and turning on a sieve having a mesh size of 1 μm). More preferably, 85% by mass or more of the total particle size falls within the range of 10 to 75 μm (for example, a sieve having an opening of 75 μm and turning on a sieve of 10 μm). Is more preferably 95% by mass or more of particles having a particle diameter in the range of 1 to 100 μm (for example, a sieve having a mesh opening of 100 μm and a mesh opening of 1 μm is turned on). It is most preferable that the mass% or more falls within the range of 10 to 75 μm in particle diameter (for example, a sieve having a mesh opening of 75 μm and turning on a sieve having a mesh size of 10 μm). If the particle size of the pulverized product exceeds the range, further pulverization of the pulverized product occurs when it is processed with an ultra-high pressure homogenizer in the subsequent process. Because it leads, it is not preferable. Further, if the particle size of the pulverized product is less than the range, it is not preferable since it only wastes time and energy for the pulverization and does not improve the extraction efficiency when processed with an ultra-high pressure homogenizer in the subsequent step. The pulverized product is preferably stored in a frozen state until a subsequent step is performed. According to this, deterioration and deterioration of the content component of bee products can be suppressed.

(2) Water addition step (S2)
Water is added to the pulverized product obtained in the freeze pulverization step (S1) to obtain a suspension. The amount of water added is preferably about 400 to 1000 mL and more preferably about 500 to 800 mL with respect to 100 g of the pulverized product. If the amount of water added is outside the above range, the efficiency of extracting the contents of the bee products tends to deteriorate, such being undesirable.

  As the water to be added, it is preferable to use active reduced water obtained by passing drinking water through an ion exchange resin to remove minerals, then contacting with obsidian to obtain reduced water, and further contacting with tourmaline. More specifically, active reduced water having a pH of 7 ± 0.5 and an oxidation-reduction potential of +250 to −250 mV obtained by passing through a resin tank, an obsidian cartridge, and a tourmaline-dispersed ceramic cartridge described below in this order is used. It is preferable to use it.

<Resin tank>
The method of removing minerals through an ion exchange resin can be conventionally performed by methods well known to those skilled in the art. For example, a strong acidic cation resin such as a sulfonated styrene vinyl benzene spherical copolymer is put into a tank of about 30 L and drinking water is passed therethrough, and iron, copper, calcium, which are cations contained in the drinking water, Magnesium or the like can be adsorbed on the resin, sodium can be released from the cation exchange resin, and the water can be softened. What is necessary is just to set suitably the kind of ion exchange resin, the capacity | capacitance of a resin, etc. of the range well-known to those skilled in the art.

<Obsidian cartridge>
Obsidian is crushed to an appropriate size, and the obsidian is placed in a holding container having a water circulation hole or directly in a cartridge, and the water passed through the resin tank is brought into contact therewith. Thereby, from the surface of obsidian, electrons are supplied into the water at any time, and the water can be reduced water. In addition, chlorine in water can be ionized and non-brominated. Furthermore, the cluster structure of water can be cut and monodispersed by the action of far infrared rays emitted by obsidian.

<Tourmaline dispersed ceramic cartridge>
By pulverizing tourmaline to a thickness of several millimeters, mixing 10 parts by weight with 90 parts by weight of an insulating ceramic extracted from basalt, and processing at about 800-1100 ° C. A ceramic sphere having a diameter of about 3 mm containing 10% by mass of tourmaline can be obtained. The insulating ceramic is used for the purpose of preventing tourmalines from being too close to each other and electrodes having opposite signs canceling each other out. A range of ones may be used. The tourmaline-dispersed ceramic sphere is placed in a holding container having a water circulation hole or directly in a cartridge, and the water passed through the obsidian cartridge is passed from the bottom to the top. Is stirred at optimum conditions. This allows contact with many water molecules. The surface electric field of the tourmaline-dispersed ceramic sphere is limited to a range of 10 to 20πm, but within that range, it exhibits a strong electrostatic field, and passes through this, preferably imparts a hydroxyl group to monodispersed water, Hydroxyl negative ions (H ₃ O ₂ ⁻ ) can be formed. Moreover, if harmful metals are contained, they can be adsorbed on the surface of the ceramic sphere and removed from the water by the electrodeposition phenomenon.

In the active reduced water thus obtained, the water molecules are monodispersed and form hydroxyl anions (H ₃ O ₂ ⁻ ), so that they have high reducing properties and surface active-like properties. It is also excellent. Therefore, the process after a hydration process can be performed, suppressing deterioration and deterioration of the content component of bee products by the high reducibility of the active reduction water. Moreover, the efficiency of extracting the content component of bee products can be further improved by the surface active-like characteristics of the active reduced water.

(3) Extraction step (S3)
The suspension obtained in the hydration step (S2) is treated with an ultrahigh pressure homogenizer to extract the content components of the bee product.

  The treatment with the ultra-high pressure homogenizer can be performed by means such as a high-pressure homogenizer disclosed in, for example, Japanese Patent No. 4594744, Japanese Patent No. 4966344, Japanese Patent No. 5165691, and the like. In this apparatus, a raw material is suspended in a liquid, and the pressure is increased by a piston / cylinder mechanism, and the material is finely homogenized by passing it through a small-diameter orifice at a high speed. According to this apparatus, the suspension containing the raw material can be processed to an ultra-high pressure of 2000 atm or more, and by passing a small-diameter orifice at a high speed from the high-pressure state, a high pressure difference generated at that time, Thus, the raw material can be finely homogenized to an average particle size of 10 microns or less, while applying little load to the components contained in the raw material, and thus suppressing denaturation and deterioration. Therefore, while suppressing the alteration and deterioration of the content components of the bee products, the content of the bee products can be improved by, for example, promoting the separation of the pollen outer membrane and its content, or promoting the fine homogenization of the propolis components. Ingredients can be extracted.

  The bee product extract obtained as described above can be appropriately concentrated, dried or powdered by an appropriate drying means. In addition, tablets, granules, powders, liquids, powders, granules, capsules, jelly-forms, cream-forms, foam-forms, ointments in combination with base materials and carriers that are acceptable for living organisms as appropriate Or the like.

  On the other hand, in FIG. 2, the flowchart of other embodiment of the manufacturing method of the bee product extract of this invention is shown. In this embodiment, in the embodiment described in FIG. 1 above, a step of removing coarse particles from the treatment liquid obtained in the extraction step (S3), mixing cyclodextrin, and forming a dry powder. Including. This will be specifically described below as a powdering step (S4).

(4) Powdering step (S4)
In the treatment liquid that has undergone the freeze pulverization step (S1) to the extraction step (S3), coarse particles that have not been processed in those steps or that have occurred during those steps remain. In the present invention, the coarse particles are preferably removed by a filter or the like. According to this, quality stability can be improved. There is no restriction | limiting in particular in the magnitude | size of openings, such as a filter, For example, a thing with a hole diameter of about 25-250 micrometers path | pass can be used. In addition, it is preferable to prepare filters with different pore diameters and apply the filters in two or more stages, such as an initial pore diameter of 150 μm pass and a second pore diameter of 50 μm pass. According to this, clogging of the filter is prevented and coarse particles can be efficiently removed. Further, it is preferable to perform filtration while applying a shearing force due to friction to the treatment liquid component remaining on the filter during filtration. According to this, clogging of the filter is prevented and coarse particles can be efficiently removed.

  Moreover, in the process liquid which passed through the freeze pulverization process (S1)-extraction process (S3), there are not a few which exist unstablely among the components extracted at those processes. Therefore, in this invention, it is preferable to mix a cyclodextrin with the process liquid obtained at the extraction process (S3). According to this, the component which exists unstable can be stabilized by inclusion with cyclodextrin. In addition, the efficiency of absorption and utilization into the body of a human or the like can be further improved. At this time, the mixing ratio of cyclodextrin is not particularly limited, but it is preferable to add 30 to 80 parts by mass with respect to 100 parts by mass of the dry solid content of the treatment liquid obtained in the extraction step (S3). It is more preferable to add -60 mass parts. Examples of the cyclodextrin include α-cyclodextrin having a degree of polymerization of 6, β-cyclodextrin having a degree of polymerization of 7, and γ-cyclodextrin having a degree of polymerization of 8. Moreover, the derivative | guide_body in which substituents, such as methylation, maltosylation, hydroxypropylation, and hydroxybutylation, were introduce | transduced may be sufficient. The cyclodextrin is preferably mixed after removing the coarse particles in order to eliminate non-specific binding with the coarse particles. According to this, the efficiency of inclusion can be improved. Further, after mixing the cyclodextrin, coarse particles may be further removed through a filter having an appropriate pore size.

  In the powdering step (S4), as described above, coarse particles are removed from the treatment liquid obtained in the extraction step (S3), and cyclodextrin is mixed to form a dry powder. By making it dry powder, the storage stability is good and it can be made a material that is easy to prepare even when blended with other materials. There is no restriction | limiting in particular in the method of dry powdering, It can carry out by the method well-known to those skilled in the art conventionally. Examples thereof include hot air drying, spray drying, drum drying, vacuum drying, freeze drying, and the like. Of these, lyophilization is preferred because of its low thermal denaturation.

  After dry powdering, it is preferable to sieve through a sieve. For example, it is preferable that 80% by mass or more of the whole is prepared in the form of a powder that passes 60 mesh (aperture 250 μm) using a standard sieve according to JIS standard, and 90% by mass or more of the standard sieve according to JIS standard is used. More preferably, it is prepared in the form of a powder that passes 60 mesh (aperture 250 μm).

  The bee product extract produced by the method of the present invention is an extract that contains abundant contents of bee products such as pollen and propolis in a form that is effectively absorbed and utilized in the body of humans, etc. Therefore, various beneficial effects on the living body can be expected. Thus, for example, health foods, functional foods, nutritional supplements, supplements, food additives, pharmaceuticals, quasi drugs, animal health foods, functional animal foods, animal nutritional supplements, animal supplements, feed It is suitable as a material used in various product forms such as additives, veterinary drugs, quasi-drugs for animals, or in combination with these products.

Example 1
A 12 kg pollen load was frozen with liquid nitrogen and pulverized with an impact pulverizer such that the particle size became 1 to 100 μm.

  According to the method described above, minerals are removed by passing drinking water through an ion exchange resin to this pulverized product, and then contacted with obsidian to form reduced water, and then 72 L of active reduced water obtained by contacting with tourmaline is added and mixed. The mixture was finely homogenized (emulsified) at a treatment pressure of 240 MPa using an ultra-high pressure homogenizer.

  The treatment solution thus obtained was dried and powdered by freeze-drying to obtain 6 kg of pollen load extract.

  This pollen loaded extract had a uniform fine powder, smooth texture and good flavor.

Example 2
The treatment liquid by the ultrahigh pressure homogenizer in Example 1 was filtered through a filter having a pore size of 150 μm, and the filtrate was further filtered through a filter having a pore size of 50 μm to obtain a filtrate from which coarse particles were removed.

  6 L of α-cyclodextrin was added to and mixed with 12 L of this filtrate (solid content concentration 14% by mass), and lyophilized to obtain 7.7 kg of pollen load extract.

  This pollen loaded extract had a uniform fine powder, smooth texture and good flavor.

Example 3
12 kg of propolis was frozen with liquid nitrogen and pulverized with an impact pulverizer such that the particle size became 1 to 100 μm.

  According to the method described above, minerals are removed by passing drinking water through an ion exchange resin to this pulverized product, and then contacted with obsidian to form reduced water, and then 72 L of active reduced water obtained by contacting with tourmaline is added and mixed. The mixture was finely homogenized (emulsified) at a treatment pressure of 240 MPa using an ultra-high pressure homogenizer.

  The treatment solution thus obtained was dried and powdered by freeze-drying to obtain 6 kg of propolis extract.

  This propolis extract had a uniform fine powder, smooth texture and good flavor. In addition, it was easy to drink with little irritation when eating.

Example 4
The treatment liquid using the ultrahigh pressure homogenizer in Example 3 was filtered through a filter having a pore size of 150 μm, and the filtrate was further filtered through a filter having a pore size of 50 μm to obtain a filtrate from which coarse particles were removed.

  6 L of α-cyclodextrin was added to and mixed with 12 L of this filtrate (solid content concentration 14% by mass), and lyophilized to obtain 7.7 kg of propolis extract.

  This propolis extract had a uniform fine powder, smooth texture and good flavor. In addition, it was easy to drink with little irritation when eating.

S1 Freezing and grinding step S2 Water addition step S3 Extraction step S4 Powdering step

Claims (6)

  Freezing and pulverizing step of freezing and pulverizing bee products with liquefied gas, hydrolyzing step of adding water to the obtained pulverized product to obtain a suspension, and treating the obtained suspension with an ultra-high pressure homogenizer A method for producing a bee product extract, comprising an extraction step of extracting a content component of the bee product.   The method for producing a bee product extract according to claim 1, further comprising a powdering step of removing coarse particles from the treatment liquid obtained in the extraction step, mixing cyclodextrin, and forming a dry powder.   The hydration step is carried out by adding drinking water through an ion exchange resin to remove minerals, then bringing it into contact with obsidian to obtain reduced water, and further adding active reduced water obtained by contacting with tourmaline. Item 3. A method for producing a bee product extract according to Item 1 or 2.   The method for producing a bee product extract according to any one of claims 1 to 3, wherein in the extraction step, the suspension is treated with an ultra-high pressure homogenizer of 2000 atm or higher.   The method for producing a bee product extract according to any one of claims 1 to 4, wherein the bee product is pollen cargo and / or propolis. A bee product extract produced by the method according to any one of claims 1 to 5.