JP2016132641A - Food taking method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a food taking method in which an effect provided by food containing useful components derived from Gramineae plant seeds is maximally enhanced.SOLUTION: In a food taking method of the present invention, food containing a peak component in distribution chromatography of alcohol extract from Gramineae plant seeds as an active ingredient is fed to a living body requiring the food within 4 hours after awakening from a sleep. The Gramineae plant is preferably Triticum. The alcohol is preferably ethanol. It is preferable that the peak component contains alkylresorcinol represented by the following general formula (I).SELECTED DRAWING: None

Description

  The present invention relates to a method for ingesting foods containing useful ingredients derived from grass seeds.

  In recent years, an increasing number of patients have a pathological condition called metabolic syndrome in which symptoms such as diabetes, hypertension, and arteriosclerosis overlap. It is thought that obesity is deeply involved in the metabolic syndrome. Obesity refers to a state in which fat is excessively accumulated due to an increase in the number of fat cells or an enlargement of the fat cells due to excessive intake of energy due to overeating or a decrease in energy consumption due to lack of exercise. Adipose cells are a huge tissue that occupies more than 10% of the human body by weight. In addition to storing energy as neutral fat, adipocytokine (adipose tissue-derived physiologically active substance) is secreted to maintain biological homeostasis. It is believed that they are actively participating. Various adipocytokines are secreted from hypertrophic fat cells that have accumulated fat excessively, and as a result, insulin resistance, hypertension, hyperlipidemia, and the like are induced. In order to control obesity and metabolic syndrome, it is important to elucidate the mechanism of differentiation of fat precursor cells into hypertrophic adipocytes and the mechanism of fat accumulation and to suppress them.

  Patent Document 1 describes the relationship between the secretion promoting action of adiponectin, which is a kind of adipocytokine, the action of inhibiting the differentiation of preadipocytes, and the suppression of fat accumulation in adipocytes, and changes in gene expression of anti-obesity related genes and blood The relationship between adiponectin and neutral fat is suggested, and it is effective to induce secretion of adiponectin in the prevention or treatment of arteriosclerosis, obesity, diabetes, etc., and a specific component extracted from a specific plant Induces the secretion of adiponectin, and those containing a resorcinol derivative as the specific component are disclosed.

  The present inventors previously proposed an anti-obesity agent containing as an active ingredient a peak component of partition chromatography of an alcoholic extract of a grass plant seed as an anti-obesity agent having an excellent anti-obesity effect and high safety ( Patent Document 2). According to the anti-obesity agent described in Patent Document 2, differentiation of preadipocytes into hypertrophic adipocytes and accumulation of fat in the cells are effectively suppressed. Patent Document 2 does not specifically mention the intake timing of the anti-obesity agent described in Patent Document 2.

JP 2005-68132 A JP 2013-40108 A

  The subject of this invention is related with the food intake method which can heighten the effect by the foodstuff containing the useful ingredient derived from a Gramineae plant seed to the maximum.

  The present invention relates to food intake, wherein a food containing a peak component of partition chromatography of an alcohol extract of a grass plant seed as an active ingredient is ingested within 4 hours after a living body that needs it wakes up from sleep. Is the method.

  The present invention also includes a food containing, as an active ingredient, a peak component of partition chromatography of an alcoholic extract of a grass seed and an instruction for the food, wherein the food is used to prevent obesity. Or a commercial package that describes or can be used for improvement and that the food is consumed within 4 hours after waking from sleep.

  According to the food intake method of the present invention, the action and effect of a food containing a useful ingredient derived from a grass family seed is maximized. The foods used in the present invention include those having an adipocyte differentiation inhibitory action and a fat accumulation inhibitory action, and these actions and effects are maximized by the implementation of the food intake method of the present invention. It can lead to the prevention or improvement of obesity in mammals including the same.

FIG. 1 is a chromatogram when a peak component of partition chromatography of a wheat ethanol extract used in Examples is obtained. FIG. 2 is a graph showing the weight gain rate of mice in Examples and Comparative Examples. FIG. 3 is a graph showing blood leptin concentrations in mice in Examples and Comparative Examples.

  The food used in the present invention is a specific food containing, as an active ingredient, a peak component of partition chromatography of an alcohol extract of a grass plant seed. This peak component contains one or more alkylresorcinol represented by the following general formula (I). The alkylresorcinol represented by the following general formula (I) has an adipocyte differentiation inhibitory action and a fat accumulation inhibitory action, and a specific food containing the same has an effect of preventing or improving obesity. The food intake method of the present invention includes a method implemented for so-called non-medical purposes for the purpose of prevention or improvement of bulimia or obesity, health maintenance, and beauty.

Regarding R ₁ in the general formula (I), examples of the saturated alkyl group having 15 to 25 carbon atoms include n-pentadecyl, n-heptadecyl, n-nonadecyl, n-henicosyl, n-tricosyl, n- Examples include linear ones such as pentacosyl and n-heptacosyl, and besides these, branched or cyclic ones may be used. Among these, a saturated alkyl group having 15 to 23 carbon atoms is preferable.

Moreover, regarding R ₁ in the general formula (I), examples of the unsaturated alkyl group having 15 to 25 carbon atoms include those corresponding to the saturated alkyl group having 15 to 25 carbon atoms. There is no restriction | limiting in particular in the number and position of the unsaturated bond contained in an unsaturated alkyl group.

In the general formula (I), R ₂ is preferably a hydrogen atom, and R ₁ is preferably bonded to R ₂ at the para position.

Specific examples of the alkylresorcinol represented by the general formula (I) include the following.
1,3-dihydroxy-5-n-pentadecylbenzene (C15: 0)
1,3-dihydroxy-5-n-heptadecylbenzene (C17: 0)
1,3-dihydroxy-5-n-nonadecylbenzene (C19: 0)
1,3-dihydroxy-5-n-henicosylbenzene (C21: 0)
1,3-dihydroxy-5-n-tricosylbenzene (C23: 0)
1,3-dihydroxy-5-n-pentacosylbenzene (C25: 0)

As a preferred example of the specific food used in the present invention, one containing the following 6 types of alkylresorcinol can be mentioned.
1) Alkyl resorcinol (hereinafter, also referred to as AR15) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 15 carbon atoms.
2) Alkyl resorcinol (hereinafter, also referred to as AR17) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 17 carbon atoms.
3) Alkyl resorcinol (hereinafter, also referred to as AR19) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 19 carbon atoms.
4) Alkyl resorcinol (hereinafter, also referred to as AR21) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 21 carbon atoms.
5) Alkyl resorcinol (hereinafter, also referred to as AR23) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 23 carbon atoms.
6) Alkyl resorcinol (hereinafter, also referred to as AR25) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 25 carbon atoms.

Particularly preferred as AR15 is one in which R ₁ is a saturated alkyl group having 15 carbon atoms and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-pentadecylbenzene (C15 : 0).
Particularly preferred as AR17 is one in which R ₁ is a saturated alkyl group having 17 carbon atoms and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-heptadecylbenzene (C17 : 0).
Particularly preferred as AR19 is one in which R ₁ is a saturated alkyl group having 19 carbon atoms and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-nonadecylbenzene (C19 : 0).
Particularly preferred as AR21 is one in which R ₁ is a saturated alkyl group having 21 carbon atoms and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-henecosylbenzene ( C21: 0).
Particularly preferred as AR23 is one in which R ₁ is a saturated alkyl group having 23 carbon atoms and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-tricosylbenzene (C23 : 0).
Particularly preferred as AR25 is one in which R ₁ is a C 25 saturated alkyl group and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-pentacosylbenzene (C25 : 0).

In the specific food used in the present invention, the contents of AR15, AR17, AR19, AR21, AR23, and AR25 are within the following ranges, respectively, from the viewpoint of further improving the action effect of the specific food, particularly the anti-obesity effect. It is preferable.
The content of AR15 is preferably 0.1 to 10.0% by mass, more preferably 0.1 to 5.0% by mass, and particularly preferably 0.5 to 1.5% by mass in the specific food used in the present invention. %.
The content of AR17 is preferably 1.0 to 20.0% by mass, more preferably 5.0 to 15.0% by mass, and particularly preferably 8.0 to 12.0% by mass in the specific food used in the present invention. %.
The content of AR19 is preferably 25.0 to 40.0% by mass, more preferably 27.5 to 37.5% by mass, and particularly preferably 30.0 to 35.0% by mass in the specific food used in the present invention. %.
The content of AR21 is preferably 40.0 to 55.0% by mass, more preferably 42.5 to 52.5% by mass, and particularly preferably 45.0 to 50.0% by mass in the specific food used in the present invention. %.
The content of AR23 is preferably 1.0-15.0 mass%, more preferably 2.5-12.5 mass%, particularly preferably 5.0-10.0 mass% in the specific food used in the present invention. %.
The content of AR25 is preferably 0 to 5.0% by mass, more preferably 0 to 2.0% by mass, and particularly preferably 0 to 1.5% by mass in the specific food used in the present invention.

The specific food used in the present invention may contain one or more alkylresorcinols other than AR15, AR17, AR19, AR21, AR23 and AR25. Examples of the other alkylresorcinol include alkylresorcinol (hereinafter, also referred to as AR27) in which R ₁ in the general formula (I) is a saturated or unsaturated alkyl group having 27 carbon atoms. Particularly preferred as AR27 is one in which R ₁ is a saturated alkyl group having 27 carbon atoms and R ₂ is a hydrogen atom. Specifically, 1,3-dihydroxy-5-n-heptacosylbenzene ( C27: 0).

  Alkylresorcinol is known to be contained in various plants as a resorcinol lipid, which is a natural non-isoterpenoid phenolic amphiphilic compound. In addition, for example, Urushiaceae, Ginkgoaceae, Porcupineaceae, Yabukodiidae, Primulaceae, Stigmaceae, Iridaceae, Araceae, Artemisia, Asteraceae, Legume, and the like are known. Among these plants, since gramineous plants have been studied for alkylresorcinol as an edible active ingredient, in the present invention, as a source of alkylresorcinol represented by the general formula (I), Employs gramineous plants.

  The gramineous plant that can be used as a source of the alkylresorcinol represented by the general formula (I) is not particularly limited, and examples thereof include wheat, durum wheat, rye, rye wheat, barley, oats, and wheat. Examples include cereals such as corn, rice, millet, millet, millet, and the like can be used alone or in combination of two or more. Among these cereals, wheat plants such as wheat and durum wheat are particularly preferable from the viewpoint of obtaining high activity, and wheat is particularly preferable. The gramineous plant seed may be any form of gramineous plant seed, for example, gramineous plant seed (preferably seed hull; moss) itself; a product obtained by cutting, crushing or pulverizing the gramineous plant seed; It may be a product obtained by drying the grass plant seed; a product obtained by drying or crushing or powdering the grass seed. Preferable examples including grass seed hulls include bran, powder, rice husk, bran and the like, and seeds with hulls.

  The method for extracting grass seeds with alcohol is not particularly limited, and examples thereof include a supercritical fluid extraction method in addition to a method of immersing, stirring, or refluxing various kinds of grass seeds in alcohol. In the case of the method of immersing, stirring or refluxing grass seeds in alcohol, the extraction temperature (alcohol liquid temperature) is preferably 2 to 100 ° C., the extraction time is preferably 0.5 to 72 hours, and the amount of alcohol used is 50-2000 mass parts is preferable with respect to 100 mass parts of gramineous plant seeds.

  Examples of the alcohol used for the extraction of grass seeds include monovalent lower alcohols (preferably those having 1 to 4 carbon atoms) such as methanol, ethanol, n-propanol, isopropanol, and n-butanol, and 1 , 3-butylene glycol, propylene glycol, polyhydric alcohols such as glycerin, and the like, which are liquids at room temperature (25 ° C.). Among these alcohols, ethanol is preferable from the viewpoint of operability and environmental properties. In addition, as alcohol used for the extraction of gramineous plant seeds, water-containing ethanol containing aqueous components (water, pure water, distilled water, tap water, acidic water, alkaline water, neutral water, etc.) other than alcohol Can also be used. The alcohol content in the hydrous alcohol is usually 70% by volume or more, preferably 80% by volume or more, more preferably 90% by volume or more.

  The alcoholic extract of gramineous seeds is purified by partition chromatography. The partition chromatography may be of any type as long as it is a technique for obtaining the active ingredient of the specific food used in the present invention, that is, the alkylresorcinol represented by the general formula (I), but a nonaqueous solvent is used as the mobile phase. A normal phase chromatography method is preferable, and a known method such as an open column method, a medium pressure column method, or a high performance liquid chromatography can be appropriately selected.

  As the mobile phase in the partition chromatography of the alcoholic extract of the grass seed, monovalent lower alcohols such as methanol, ethanol, n-propanol, isopropanol, n-butanol (preferably having 1 to 4 carbon atoms) And alcohols that are liquid at room temperature (25 ° C.) such as polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, and glycerin; ethers such as diethyl ether and propyl ether; esters such as butyl acetate and ethyl acetate; acetone And ketones such as ethyl methyl ketone; hexane; methylene chloride; acetonitrile; and chloroform. These solvents can be used alone or in combination of two or more. When a plurality of solvents are combined to form a mobile phase, an isocratic mode in which the mixing ratio of the plurality of solvents is kept constant during partition chromatography (purification of the gramineous alcohol extract), or A gradient mode in which the mixing ratio is changed may be used. Any carrier can be used as the carrier in the partition chromatography as long as it can carry and release the target active ingredient, and generally includes silica gel, polyacrylamide gel, dextran gel and the like. . The detection wavelength in the partition chromatography of the alcoholic extract of the grass plant seed may be 170 to 320 nm, preferably 190 to 280 nm.

Examples of partition chromatography suitable for purification of an alcohol extract (preferably an ethanol extract) of a grass seed (preferably a plant of the genus Wheat) include the following partition chromatography A and B.
Partition chromatography A: using a medium pressure column method (medium pressure chromatography) using silica gel as a carrier and a hexane-ethyl acetate mixed solvent as a mobile phase, and during the execution of the partition chromatography, -Changed from "relatively high hexane content in ethyl acetate mixed solvent" to "relatively low hexane content in hexane-ethyl acetate mixed solvent" And a peak component at a detection wavelength of 254 nm is fractionated.
Partition chromatography B: Using high performance liquid chromatography (HPLC) using silica gel as a carrier and methanol as a mobile phase, a peak component at a detection wavelength of 215 nm is fractionated.

  In the specific food used in the present invention, the content of the active ingredient (alkylresorcinol represented by the general formula (I)) is not particularly limited, and the specific food may be composed only of the active ingredient, Or you may contain other components other than the said active ingredient in the range which does not prevent the effect by the said active ingredient. Other ingredients include, for example, various cosmetic and nutritional ingredients that can be incorporated into general foods, excipients, disintegrants, binders, lubricants, surfactants, water-soluble polymers, diluents, Osmotic pressure adjuster, pH adjuster, emulsifier, preservative, buffer, stabilizer, antioxidant, thickener, UV absorber, activity enhancer, colorant, sweetener, flavoring agent, flavoring agent, acidity A material etc. are mentioned, These 1 type can be used individually or in combination of 2 or more types.

  The form of the specific food used in the present invention is not particularly limited, and may be solid, semi-solid, or liquid, and examples thereof include tablet form, pill form, capsule form, liquid form, syrup form, powder form, and granule form. Beverages are also included in the specific food used in the present invention, and examples of the specific food in the form of beverage include tea beverages, coffee beverages, milk beverages, fruit juice beverages, carbonated beverages, alcoholic beverages, and soft drinks. Specific forms of the specific food used in the present invention include breads, noodles, jelly-like foods and various snacks, baked goods, cakes, chocolate, gum, rice cake, tablets, capsules, soups, dairy products, frozen products. Examples include foods, instant foods, supplements, other processed foods, seasonings, and materials thereof.

  The specific foods used in the present invention include all foods, in addition to general foods including so-called health foods, health functional foods such as foods for specific health and nutritional functional foods defined in the health functional food system of the Ministry of Health, Labor and Welfare, supplements Etc., and also feeds fed to animals.

  The present invention relates to the above-described method for ingesting a specific food, and one of its main features is that within 4 hours, preferably within 3 hours, more preferably after a living body that requires the specific food awakens from sleep. The point of ingesting within 2 hours is mentioned. Thereby, as demonstrated in the Example mentioned later, the effect by a specific foodstuff, especially an anti-obesity effect are maximized, and prevention or improvement of obesity can be promoted greatly.

  The “living body” as used in the present invention includes mammals, and includes humans, for example, dogs, cats, mice, rats, rabbits, cows, horses, monkeys, and the like. That is, the food intake method of the present invention can be applied not only to humans but also to pets (companion animals), livestock, and the like, and can be implemented for non-medical purposes on all mammals.

  In addition, “sleep” as used in the present invention is a natural state that is accompanied by a decrease in the response to surrounding stimuli and is not conscious but can be easily awakened, and when there are a plurality of such states within 24 hours a day Of these, only the one in which the state lasts for the longest time corresponds to “sleep” in the present invention. That is, the sleep in the present invention is only once in 24 hours a day, and for example, apart from sleep (sleeping) over several hours that a person usually takes at night, a comparatively short time taken in the daytime or the like. The so-called nap over the range is not sleep in the present invention. In the food intake method of the present invention, the sleep time is not particularly limited, but is preferably 3 hours or more, and more preferably 4 to 10 hours.

  In the food intake method of the present invention, the specific food described above may be taken within 4 hours after waking up from sleep, and may be taken once within 4 hours after waking up. May be taken separately.

  In addition, in the food intake method of the present invention, the intake of the specific food may be adjusted as appropriate according to the age or weight of the ingested living body or the form of the specific food, and is not particularly limited. The intake of the active ingredient is preferably adjusted to be 0.01 to 10 g per person per day. That is, as a preferred embodiment of the food intake method of the present invention, within 10 hours after waking up from sleep, 0.01 to 10 g of the specific food per day is divided into one or more times and allowed to be ingested by humans. An embodiment is mentioned.

In addition, when a living body other than a human is targeted, the preferred intake of the specific food is as follows. That is, as a preferred embodiment of the food intake method of the present invention, within 4 hours after waking up from sleep, the following specific amount of the specific food is divided into one time or a plurality of times and taken into a living body other than human beings. Is mentioned.
For dogs: 0.2 mg to 20 g per day
・ For cats: 1 mg to 10 g per day
For hamsters: 0.02 mg to 40 mg per day
-For rabbits: 0.4 mg to 4 g per day

  In addition, the period for carrying out the method for ingesting the food of the present invention is not particularly limited, but it is preferably carried out continuously for a certain period. From the viewpoint of sufficiently exhibiting the above-described effects of the specific food, particularly the anti-obesity effect, 2 It is preferable to carry out continuously for more than a week, particularly for more than 7 weeks.

  The present invention includes a commercial package including the above-described specific food and instructions for the specific food. The instructions state that the specific food can or should be used for the prevention or amelioration of obesity and that the specific food is consumed within 4 hours after waking from sleep. The form of this commercial package is not particularly limited, for example, a form in which instructions are affixed to a packaging container containing specific food, a form in which instructions are enclosed with the specific food in the packaging container, or the packaging container itself The form in which the description content of the instruction is printed on (the packaging container is in the form of the instruction) and the like.

The present invention includes the following <1>.
<1> Prevention of obesity, wherein a food containing a peak component of partition chromatography of an alcohol extract of a grass plant seed as an active ingredient is ingested within 4 hours after a living body in need thereof awakes from sleep or How to improve.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated further in detail, this invention is not restrict | limited by an Example.

By the following <extraction purification method>, the peak component of the partition chromatography of wheat ethanol extract containing the alkylresorcinol represented by the general formula (I) was obtained. The composition of the obtained peak component is as follows.
-1,3-dihydroxy-5-n-pentadecylbenzene (C15: 0) 1.2 mass%.
-1,3-dihydroxy-5-n-heptadecylbenzene (C17: 0) 10.9 mass%.
-1,3-dihydroxy-5-n-nonadecylbenzene (C19: 0) 33.9 mass%.
-1,3-dihydroxy-5-n-henicosylbenzene (C21: 0) 46.4 mass%.
-7.5 mass% of 1, 3- dihydroxy-5-n-tricosyl benzene (C23: 0).
-1,3-dihydroxy-5-n-pentacosylbenzene (C25: 0) 0.1 mass%.

<Extraction purification method>
Ethanol in an amount of 5 times by mass was added to wheat bran, and the mixture was extracted by stirring for 16 hours under conditions of 600 rpm and room temperature. The extract was filtered to remove unnecessary substances and an ethanol extract was collected, and then ethanol was distilled off to obtain a wheat ethanol extract.
The wheat ethanol extract was then purified by medium pressure chromatography. Medium pressure chromatography conditions are as follows. The peak component appearing 31 to 36 minutes after the start of elution was collected, and the solvent was distilled off to obtain the peak component of partition chromatography of the wheat ethanol extract.
(Medium pressure chromatography conditions)
Column: silica gel (injection column 3 L, high flash column 5 L, 60 mm, 40 μm, manufactured by Yamazen Corporation)
・ Mobile phase: Hexane / ethyl acetate mixed solvent (volume ratio) = 9/10 at 90/10, 15 minutes at 80/20, 16 minutes at 60/40 ・ Detection wavelength: 254 nm

In addition, the purification of the wheat ethanol extract in the above <Extraction and purification method> can be performed by HPLC instead of medium pressure chromatography. In this case, methanol is added to the wheat ethanol extract to prepare a methanol additive solution having a concentration of 200 ug / ml, and the methanol additive solution is passed through a filter having a pore size of 0.45 μm. Are HPLC samples. HPLC conditions are as follows.
(HPLC conditions)
Column: silica gel (ODS-80A, 5 μm, 4.6 × 250 mm, manufactured by GL Sciences Inc.)
Guard column: ODS-80A, 5 μm, 4.6 × 50 mm,
-Column temperature: 30 ° C
-Mobile phase: 100% methanol
・ Detection wavelength: 215 nm

[Example 1]
As a high fat high sucrose diet (hereinafter also referred to as HFHSD), F2HFHSD (manufactured by Oriental Yeast Co., Ltd.) is used, and 0.5% by mass of the peak component of the above-mentioned wheat ethanol extract partition chromatography is added to HFHSD. Thus, a feed corresponding to the above-mentioned specific food (hereinafter also referred to as HFHSDAR) was prepared.
C57BL / 6JJmsSlc strain mice (11-week-old male, Japan SLC, Inc.) bred under a light-dark cycle of 12 hours light period and 12 hours dark period (lit at 0:00, unlit at 12:00) HFHSDAR was ingested for 8 weeks. During the test period, the animals were fed twice a day, one of which was fed with HFHSDAR within 4 hours after the mice woke up from sleep, and the other one was the bedtime of the mice. HFHSD was fed within 4 hours before ingestion.

[Comparative Example 1]
The procedure was the same as Example 1 except that HFHSD was used as the feed fed within 4 hours after the mice woke up from sleep. That is, during the test period, the mice were ingested only with HFHSD.

[Comparative Example 2]
Example 1 except that HFHSD was fed and consumed within 4 hours after the mice woke up from sleep, and HFHSDAR was fed and consumed within 4 hours before the mice went to sleep. .

(Evaluation test)
About the mouse | mouth of an Example and a comparative example, the weight of the mouse | mouth was measured every week during the said test period, and the weight increase rate (%) with respect to the body weight of the mouse | mouth before a test was computed. The result is shown in FIG. In addition, after completion of the test period, blood was collected from the mice of Examples and Comparative Examples, and blood leptin concentration was measured. Leptin was measured using a mouse leptin measurement kit (Morinaga Biochemical Laboratories) according to the attached protocol. The result is shown in FIG. Leptin is a hormone that suppresses eating and has a lipolytic effect. It is known that the metabolic syndrome living body has a reduced leptin control function, and leptin is secreted into the blood more than necessary, resulting in an increase in blood leptin concentration. This is called leptin resistance. There is a correlation between the pathological condition of metabolic syndrome and the blood leptin concentration, and the metabolic syndrome pathological condition tends to progress as the blood leptin concentration increases. In FIG. 3, # indicates that a significant difference between Example 1 and Comparative Example 1 was recognized at the significance level p <0.01, and * indicates that Example 1 and Comparative Example 2 It is shown that a significant difference was recognized at a significance level p <0.05.

It can be seen that Example 1 has a significantly lower body weight gain rate (see FIG. 2) and blood leptin concentration (see FIG. 3) than Comparative Examples 1 and 2, and has a high anti-obesity effect. As in Example 1, Comparative Example 2 resulted in inferior anti-obesity effect as compared to Example 1, despite regularly ingesting the peak component of the partition chromatography of the wheat ethanol extract. .
From the above, 1) the peak component of the partition chromatography of the alcoholic extract of the grass plant seed has an anti-obesity effect, and 2) the anti-obesity effect depends on the timing of ingesting the peak component (specific food) In contrast, it can be seen that it is more effective to ingest the living body within 4 hours after waking up from sleep.

Claims (5)

  A method of ingesting food, wherein a food containing a peak component of partition chromatography of an alcoholic extract of a grass plant seed as an active ingredient is ingested within 4 hours after the living body that needs it wakes up from sleep.   The food intake method according to claim 1, wherein the gramineous plant belongs to the genus Wheat.   The food intake method according to claim 1 or 2, wherein the alcohol is ethanol. The food intake method according to any one of claims 1 to 3, wherein the peak component contains an alkylresorcinol represented by the following general formula (I).

  A food containing a peak component of partition chromatography of an alcoholic extract of a grass plant seed as an active ingredient, and instructions for the food, wherein the food is used for prevention or amelioration of obesity. A commercial package that states that it should be obtained or used and that the food is consumed within 4 hours after waking from sleep.

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