JP2016032448A - Feeds for farmed fishes and culture method of farmed fishes - Google Patents

Feeds for farmed fishes and culture method of farmed fishes Download PDF

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JP2016032448A
JP2016032448A JP2014156641A JP2014156641A JP2016032448A JP 2016032448 A JP2016032448 A JP 2016032448A JP 2014156641 A JP2014156641 A JP 2014156641A JP 2014156641 A JP2014156641 A JP 2014156641A JP 2016032448 A JP2016032448 A JP 2016032448A
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cultured fish
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山下 浩史
Hiroshi Yamashita
浩史 山下
大典 稲井
Taisuke Inai
大典 稲井
正彦 首藤
Masahiko Shudo
正彦 首藤
正樹 山内
Masaki Yamauchi
正樹 山内
邦明 菅原
Kuniaki Sugawara
邦明 菅原
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EHIME INRYO KK
Ehime Prefecture
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Abstract

PROBLEM TO BE SOLVED: To provide feeds for farmed fishes which can add the aroma of citrus to the fish meat of farmed fishes.SOLUTION: The feeds for farmed fishes according to the embodiment of the invention is obtained by adding peel oil, which is collected from citrus peel by milling process and is rough-purified without distilling. The culture method for farmed fishes according to the embodiment of the invention comprises feeding a given amount of the peel oil to the farmed fishes as the feeds for farmed fishes added with peel oil collected from citrus peel by milling process and rough-purified without distilling.SELECTED DRAWING: Figure 5

Description

本発明は、養殖魚用の飼料及び養殖魚の養殖方法に関する。   The present invention relates to a feed for cultured fish and a method for culturing cultured fish.

従来から、ブリやマダイ等の養殖魚の養殖が盛んに行われており、様々な養殖魚用の飼料が提供されている。また、近年、養殖魚の肉質を改善したり、養殖魚の成長を促進させたりするために、色々な飼料が開発されており、例えば、植物由来成分を混合した養殖魚用飼料が提供されている。   Conventionally, cultured fish such as yellowtail and red sea bream have been actively cultivated, and various types of feed for cultured fish have been provided. In recent years, various feeds have been developed in order to improve the quality of cultured fish and promote the growth of cultured fish. For example, feed for cultured fish mixed with plant-derived components is provided.

例えば、下記特許文献1には、梅調味液を混合したマグロ用飼料が開示され、下記特許文献2には、脱塩濃縮梅酢を含有する養殖魚用飼料が開示され、下記特許文献3には、ローズヒップを含有する魚介類用飼料が開示されている。   For example, the following Patent Document 1 discloses a tuna feed mixed with a plum seasoning liquid, the following Patent Document 2 discloses a cultured fish feed containing desalted and concentrated plum vinegar, and the following Patent Document 3 discloses A feed for seafood containing rosehips is disclosed.

特開2010−246417号公報JP 2010-246417 A 特開2008−206501号公報JP 2008-206501 A 特開2008−173044号公報JP 2008-173044 A

このように、従来、養殖魚の肉質を向上させるための飼料が種々提供されているが、養殖魚に柑橘の香りを付けるための飼料は提供されていない。   As described above, various feeds for improving the quality of cultured fish have been conventionally provided, but no feed for adding a citrus scent to the cultured fish is provided.

本発明は、このような課題に鑑みてなされたものであり、養殖魚の魚肉に柑橘の香り付けをすることのできる養殖魚用飼料を提供することを目的とする。   This invention is made | formed in view of such a subject, and it aims at providing the feed for cultured fish which can scent citrus to the fish meat of cultured fish.

上記課題を解決するための本発明に係る養殖魚用飼料は、圧搾法により柑橘の果皮から採取して、蒸留することなく粗精製した果皮油を添加したことを特徴とする。   The feed for cultured fish according to the present invention for solving the above-mentioned problems is characterized in that percutaneous oil extracted from citrus peel by a pressing method and roughly purified without distillation is added.

また、本発明に係る養殖魚の養殖方法は、圧搾法により柑橘の果皮から採取して、蒸留することなく粗精製した果皮油を添加した養殖魚用飼料を、養殖魚の水揚げ直前に少なくとも7日以上に分けて所定量与える果皮油給餌工程を備えることを特徴とする。   In addition, the cultured fish culture method according to the present invention is a culture fish feed that is collected from citrus peel by the compression method and added with crude oil that has been roughly purified without distillation, and at least 7 days or more immediately before landing the cultured fish It is characterized by comprising a skin oil feeding step for dividing and giving a predetermined amount.

本発明に係る養殖魚用飼料によれば、養殖魚の魚肉に柑橘果皮油成分を移行させて、柑橘の香りのする養殖魚を提供することができる。   According to the feed for cultured fish which concerns on this invention, the citrus peel oil component can be transferred to the fish meat of cultured fish, and the cultured fish which has a citrus fragrance can be provided.

また、本発明に係る養殖魚の養殖方法によれば、養殖魚の魚肉に柑橘果皮油成分を効率的に移行させて、柑橘の香りのする養殖魚を提供することができる。   Moreover, according to the cultivation method of the cultured fish which concerns on this invention, the citrus peel oil component can be efficiently transferred to the fish meat of cultured fish, and the cultured fish which has a citrus fragrance can be provided.

図1は、本発明の実施形態に係る飼育試験1の各試験区の平均魚体重の推移を示す図である。FIG. 1 is a diagram showing the transition of the average fish weight in each test section of the breeding test 1 according to the embodiment of the present invention. 図2は、本発明の実施形態に係る飼育試験1の各試験区における果皮油成分の含有量を分析した結果を示す図である。FIG. 2 is a diagram showing the results of analyzing the content of the peel oil component in each test section of the breeding test 1 according to the embodiment of the present invention. 図3は、本発明の実施形態に係る飼育試験2の試験結果を示す図である。FIG. 3 is a diagram showing test results of breeding test 2 according to the embodiment of the present invention. 図4は、本発明の実施形態に係る飼育試験3の試験結果を示す図である。FIG. 4 is a diagram showing test results of breeding test 3 according to the embodiment of the present invention. 図5は、本発明の実施形態に係る各飼育試験において養殖魚に与えられる伊予柑果皮油の総給餌量を示す図である。FIG. 5 is a diagram showing the total amount of Iyo citrus peel oil given to cultured fish in each breeding test according to the embodiment of the present invention.

以下、本発明の実施の形態について説明する。本実施形態では、柑橘として伊予柑を使用し、伊予柑の果皮から採取し、加熱して蒸留することなく粗精製した伊予柑果皮油(粗精製精油)をブリ及びマダイの養殖魚用飼料(モイストペレット)に添加した場合を例に挙げて説明する。   Embodiments of the present invention will be described below. In this embodiment, Iyokan is used as a citrus fruit, and Iyokan peel oil (crude refined essential oil), which is collected from the skin of Iyokan and is roughly refined without heating and distillation, is a feed for cultured fish of yellowtail and red sea bream ( The case of adding to a moist pellet will be described as an example.

このように、伊予柑の果皮から採取した伊予柑果皮油を養殖魚用飼料に混ぜることで、養殖魚の魚臭さが低減されると共に、伊予柑の香り成分が養殖魚の魚肉に移行し、ほのかに伊予柑の香りのする養殖魚を提供することができる。   In this way, mixing Iyo citrus peel oil collected from the skin of Iyokan into the feed for cultured fish reduces the fishy odor of the cultured fish, and the scent component of Iyokan migrates to the fish meat of the cultured fish. Can provide cultured fish with the scent of Iyokan.

表1は、本実施形態に係る養殖魚用飼料(実施例1)の組成を示している。実施例1は、質量比で、配合飼料(粉末)を40質量%、生魚(マアジ)を48質量%、アミエビを4質量%、フィードオイル(魚油)を6質量%、伊予柑果皮油を0.2質量%、蒸留水を1.8質量%混合して生成したモイストペレットである。   Table 1 shows the composition of the feed for cultured fish according to the present embodiment (Example 1). Example 1 has a mass ratio of 40% by mass of mixed feed (powder), 48% by mass of raw fish (maji), 4% by mass of shrimp, 6% by mass of feed oil (fish oil), and 0.2% of Iyo citrus peel oil. It is a moist pellet produced by mixing 1.8% by mass of mass% and distilled water.

Figure 2016032448
Figure 2016032448

ここで、本実施形態に係る伊予柑果皮油は、インライン搾汁機に水の噴霧装置を取り付け、伊予柑果汁を搾った残りの果皮及び残液からなる含油泥状液を遠心分離機にかけて取り出す(粗精製)ことで得られる。   Here, the Iyo citrus peel oil according to the present embodiment attaches a water spray device to the inline juicer, and takes out the oil-containing mud liquid consisting of the remaining peel and the remaining liquid obtained by squeezing the Iyo citrus juice through a centrifuge. (Crude purification).

このように、本実施形態に係る伊予柑果皮油は、精製にあたって蒸留(加熱)を行っていないので、伊予柑の果皮に含まれる精油の特定成分が飛んで失われることなく、そのまま残っている。柑橘類の果皮油は、D−リモネンを主成分とし、テルピネン(α−テルピネン、β−テルピネン、γ−テルピネン等)等の他の成分を少量含んでおり、これらの配合比の違いにより各柑橘類特有の香りを構成している。   As described above, the Iyo citrus peel oil according to the present embodiment is not distilled (heated) during the purification, so that the specific components of the essential oil contained in the Iyokan peel are not lost and are left as they are. . Citrus peel oil contains D-limonene as the main component and contains a small amount of other components such as terpinene (α-terpinene, β-terpinene, γ-terpinene, etc.). The scent of make up.

したがって、加熱により特定の精油成分が失われてしまうと、抽出した精油の香りが元の柑橘類の香りと異なる香りになってしまうおそれがあるが、本実施形態のように蒸留することなく遠心分離等の粗精製により伊予柑果皮油を抽出することで、伊予柑の香りと同じ香りのする伊予柑果皮油を得ることができる。   Therefore, if a specific essential oil component is lost due to heating, the scent of the extracted essential oil may be different from the scent of the original citrus, but it is centrifuged without distillation as in this embodiment. By extracting Iyo citrus peel oil by rough purification such as Iyo citrus peel oil, the scent of Iyokan can be obtained.

比較例1は、実施例1の伊予柑果皮油の代わりに、伊予柑果汁を搾った後の残りの果皮をそのまま粉砕して混ぜたものである。比較例1は、質量比で、配合飼料(粉末)を40質量%、生魚(マアジ)を40質量%、アミエビを4質量%、フィードオイルを6質量%、粉砕果皮を10質量%混合して生成したモイストペレットである。   In Comparative Example 1, instead of the Iyo citrus peel oil of Example 1, the remaining peel after squeezing Iyo citrus juice is pulverized and mixed as it is. Comparative Example 1 is a mass ratio of 40% by mass of mixed feed (powder), 40% by mass of raw fish (maji), 4% by mass of shrimp, 6% by mass of feed oil, and 10% by mass of ground skin. It is the produced | generated moist pellet.

すなわち、比較例1は、伊予柑果皮油を含んでいるが、伊予柑果皮油を果皮ごと粉砕して飼料に混ぜている点で実施例1と異なる。なお、比較例1の粉砕果皮には、質量比で約2.5%程度の伊予柑果皮油が含まれており、比較例1の飼料には、実施例1の0.2質量%よりも若干多い0.25質量%程度の伊予柑果皮油が含まれている。   That is, Comparative Example 1 includes Iyo citrus peel oil, but differs from Example 1 in that Iyo citrus peel oil is pulverized together with the peel and mixed with feed. The ground skin of Comparative Example 1 contains about 2.5% Iyo citrus peel oil in a mass ratio, and the feed of Comparative Example 1 has a 0.25 mass that is slightly higher than the 0.2 mass% of Example 1. About 1% Iyo citrus peel oil is included.

比較例2は、従来の一般的な養殖魚用飼料であり、質量比で、配合飼料(粉末)を40質量%、生魚(マアジ)を50質量%、アミエビを4質量%、フィードオイルを6質量%混合して生成したモイスペレットである。   Comparative Example 2 is a conventional general aquaculture fish feed, which is 40% by mass of mixed feed (powder), 50% by mass of raw fish (maji), 4% by mass of shrimp, and 6% of feed oil. It is a moisture pellet produced by mixing mass%.

続いて、実施例1、比較例1、比較例2の養殖魚用飼料を使って養殖した飼育試験1の結果について、図1及び図2を参照しながら説明する。本飼育試験1では、水揚げ時の魚体重が約5kgとなるブリの2歳魚を10尾ずつ3つの試験区に分け、各試験区において、実施例1、比較例1、比較例2の養殖魚用飼料を8週間にわたって1kgずつ40回(合計40kg)給餌した。   Then, the result of the breeding test 1 cultured using the cultured fish feed of Example 1, Comparative Example 1, and Comparative Example 2 will be described with reference to FIGS. 1 and 2. In this breeding test 1, the 2 year old fish of the yellowtail whose fish weight at the time of landing is about 5 kg is divided into 3 test sections of 10 fish, and in each test section, the culture of Example 1, Comparative Example 1 and Comparative Example 2 is carried out. Fish feed was fed 40 times per kg (total 40 kg) over 8 weeks.

図1は、飼育試験1の各試験区における平均魚体重の推移を示しており、給餌開始時と、8週間給餌後の平均魚体重を示している。図2は、飼育試験1の2週間給餌(給餌回数10回。合計10kg)後に各試験区においてサンプリングした養殖魚(ブリ)の筋肉中における伊予柑果皮油の成分(D−リモネン、γ−テルピネン)の含有量を分析(HS-GC-MS:ヘッドスペースガスクロマト質量分析)した結果を示している。   FIG. 1 shows the transition of the average fish weight in each test section of the breeding test 1, and shows the average fish weight at the start of feeding and after feeding for 8 weeks. Fig. 2 shows components of Iyo citrus peel oil (D-limonene, γ-terpinene) in the muscles of cultured fish (yellowtail) sampled in each test area after feeding for 2 weeks in breeding test 1 (10 times of feeding, total 10 kg) ) Content (HS-GC-MS: headspace gas chromatography mass spectrometry).

図1に示すように、飼育試験1の開始前には、全ての試験区において平均魚体重が約4.1kgと同じであったのに対して、8週間経過後には、実施例1及び比較例2においては、平均魚体重が4.5〜4.6kg程度と順調に養殖魚が成長しているのに対して、比較例1では、平均魚体重が約4.3kg程度と、他の試験区と比較して成長が大幅に鈍化している。   As shown in FIG. 1, before the start of breeding test 1, the average fish weight was about 4.1 kg in all test plots, whereas after 8 weeks, Example 1 and Comparative Example In 2, the average fish weight is about 4.5 to 4.6 kg, while the cultured fish is growing steadily. In Comparative Example 1, the average fish weight is about 4.3 kg, which is compared with other test areas. Growth has slowed significantly.

比較例1の試験区において体重増加率が低下するのは、比較例1では、果汁を搾った後の果皮を粉砕して含めているため、その果皮の質量分だけ相対的に飼料全体に占める配合飼料や魚(マアジ)の質量が減っていること、果皮には食物繊維が多く含まれ、果皮を食べた養殖魚の飼料摂取量が他の試験区と比べて減少すること等が起因すると考えられる。   The reason why the weight gain rate decreases in the test section of Comparative Example 1 is that, in Comparative Example 1, since the fruit skin after squeezing the fruit juice is included by pulverization, it is relatively occupied by the mass of the fruit skin. This is due to the fact that the mass of mixed feed and fish (Maji) has decreased, the peel contains a lot of dietary fiber, and the intake of cultured fish that have eaten the peel has decreased compared to other test plots. It is done.

よって、図1に示す結果から、養殖魚用飼料に伊予柑果皮油を混ぜる場合には、伊予柑果皮を粉砕したものをそのまま添加するよりも、実施例1のように、粗精製した果皮油を添加するほうが、養殖魚の成長の面で望ましいことが分かる。養殖魚の売価は、基本的に水揚げ時の魚体重に比例するため、比較例1のように、8週間にわたって飼料を与えても魚体重の増加が少ないと、養殖業者にとっては、コスト的なデメリットが大きい。   Therefore, from the results shown in FIG. 1, when Iyo citrus peel oil is mixed with the cultured fish feed, it is roughly refined as shown in Example 1 rather than adding the crushed Iyo citrus peel as it is. It can be seen that the addition of is preferable in terms of the growth of cultured fish. The selling price of aquaculture fish is basically proportional to the fish body weight at the time of landing. Therefore, as in Comparative Example 1, if the fish body weight increases little even if feed is given over 8 weeks, it is a cost disadvantage for the farmer. Is big.

また、図2に示す結果から、実施例1及び比較例1のように、伊予柑果皮油を混ぜた養殖魚用飼料をブリに与えることで、ブリの筋肉中にはD−リモネン、γ−テルピネンが含まれるようになる。すなわち、ブリの魚肉に伊予柑の香りの主成分が移行し、ブリの魚肉から伊予柑の香りがするようになる。   Further, from the results shown in FIG. 2, as shown in Example 1 and Comparative Example 1, by giving the yellowtail a feed for cultured fish mixed with Iyo citrus peel oil, D-limonene, γ- Terpinene is included. That is, the main component of the scent of Iyokan is transferred to the fish meat of the yellowtail, and the scent of Iyokan comes from the fish of the yellowtail.

また、上述したように、比較例1の飼料には、0.25質量%程度の伊予柑果皮油が含まれており、実施例1の飼料よりも伊予柑果皮油の含有量が若干多い。これに対して、図2に示すように、実施例1のほうがブリの筋肉中に含まれる伊予柑果皮油成分が多くなっている。すなわち、本実施形態のように、粉砕した果皮をそのまま飼料に混合するよりも、粗精製した伊予柑果皮油を飼料に添加したほうが、伊予柑果皮油成分を効率的に養殖魚に移行させることができる。   Further, as described above, the feed of Comparative Example 1 contains about 0.25% by mass of Iyo citrus peel oil, and the content of Iyo citrus peel oil is slightly higher than that of the feed of Example 1. On the other hand, as shown in FIG. 2, Example 1 has more Iyo citrus peel oil component contained in the muscle of yellowtail. That is, it is more efficient to transfer the Iyo citrus peel oil component to the cultured fish when the crudely refined Iyo citrus peel oil is added to the feed rather than mixing the crushed peel directly into the feed as in this embodiment. Can do.

なお、本実施形態のように粗精製した伊予柑果皮油を用いれば、果皮の場合と比べて長期保存ができ、1年を通して利用可能であり、保管スペースも小さくて済むというメリットがある。また、果皮油であれば、モイストペレットだけでなく、ドライペレットにも混合することができるといったメリットがある。   It should be noted that the use of roughly refined Iyo citrus peel oil as in the present embodiment has the advantage that it can be stored for a long period of time compared to the case of the peel, can be used throughout the year, and the storage space can be reduced. Moreover, if it is pericarp oil, there exists an advantage that it can be mixed not only with a moist pellet but with a dry pellet.

このように、実施例1に係る養殖魚用飼料を用いれば、養殖魚の成長を鈍化させることなく、一般的な飼料(比較例2)と同様に養殖魚の体重を増加させながら、魚肉に伊予柑の香り成分を効率的に移行させることができ、魚臭さを低減させてほのかに伊予柑の香りのする身を有するブリを提供することができる。すなわち、水揚げ高を減少させることなく、品質の高いブリを提供することができる。   As described above, when the cultured fish feed according to Example 1 is used, the weight of the cultured fish is increased in the same manner as general feed (Comparative Example 2) without slowing the growth of the cultured fish. The scent component can be efficiently transferred, and the fish odor can be reduced to provide a yellowtail having a faint scent of Iyokan. That is, high quality yellowtail can be provided without reducing the landing height.

次に、本実施形態に係る養殖魚用飼料の実施例2〜7について説明する。表2は、実施例2〜7及び比較例3の養殖魚用飼料の組成を示している。ここで、実施例2〜4は、ブリ用の飼料であり、実施例5〜7は、マダイ用の飼料である。   Next, Examples 2 to 7 of the feed for cultured fish according to the present embodiment will be described. Table 2 shows the composition of the feed for cultured fish of Examples 2 to 7 and Comparative Example 3. Here, Examples 2 to 4 are feeds for yellowtail, and Examples 5 to 7 are feeds for red sea bream.

Figure 2016032448
Figure 2016032448

実施例2〜7及び比較例3は、質量比で配合飼料を50質量%、魚(マアジ)を40質量%、総合ビタミン剤を2質量%含んでおり、各実施例2〜7は、さらにフィードオイル及び伊予柑果皮油を所定量ずつ含み、比較例3は、さらにフィードオイルのみを8質量%含んでいる。   Examples 2 to 7 and Comparative Example 3 contain 50% by mass of mixed feed, 40% by mass of fish (maji), and 2% by mass of a multivitamin preparation in terms of mass ratio. A predetermined amount of feed oil and Iyo citrus peel oil are included, and Comparative Example 3 further includes only 8% by mass of feed oil.

詳細には、ブリ用の養殖魚用飼料である実施例2〜4において、実施例2は、さらにフィードオイルを7.98質量%、伊予柑果皮油を0.02質量%含み、実施例3は、さらにフィードオイルを7.96質量%、伊予柑果皮油を0.04質量%含み、実施例4は、さらにフィードオイルを7.94質量%、伊予柑果皮油を0.06質量%含んでいる。   Specifically, in Examples 2 to 4, which are feed for cultured fish for yellowtail, Example 2 further includes 7.98% by mass of feed oil and 0.02% by mass of Iyo citrus peel oil, and Example 3 further includes feed. Oil contains 7.96 mass%, Iyo citrus peel oil 0.04 mass%, and Example 4 further contains 7.94 mass% feed oil and Iyo citrus peel oil 0.06 mass%.

また、マダイ用の養殖魚用飼料である実施例5〜7において、実施例5は、さらにフィードオイルを7.9質量%、伊予柑果皮油を0.1質量%含み、実施例6は、さらにフィードオイルを7.8質量%、伊予柑果皮油を0.2質量%含み、実施例7は、さらにフィードオイルを7.7質量%、伊予柑果皮油を0.3質量%含んでいる。   Moreover, in Examples 5 to 7 which are cultured fish feed for red sea bream, Example 5 further includes 7.9% by mass of feed oil and 0.1% by mass of Iyo citrus peel oil, and Example 6 further includes feed oil. 7.8% by mass, 0.2% by mass of Iyo citrus peel oil, Example 7 further contains 7.7% by mass of feed oil and 0.3% by mass of Iyo citrus peel oil.

続いて、実施例2〜4及び比較例3の養殖魚用飼料を使ってブリを養殖した飼育試験2及び実施例5〜7及び比較例3の養殖魚用飼料を使ってマダイを養殖した飼育試験3の結果について、図3及び図4を参照しながら説明する。   Subsequently, breeding test 2 in which yellowtail was cultured using the cultured fish feed of Examples 2 to 4 and Comparative Example 3, and breeding in which red sea bream was cultured using the cultured fish feed of Examples 5 to 7 and Comparative Example 3 The results of Test 3 will be described with reference to FIGS.

飼育試験2では、水揚げ時の魚体重が約5kgとなるブリを10尾ずつ4つの試験区に分け、各試験区において、実施例2、実施例3、実施例4、比較例3の養殖魚用飼料を2週間にわたって、1kgずつ10回(合計10kg)給餌した。   In breeding test 2, yellowtails with a fish weight of about 5 kg when landing are divided into four test zones of 10 fish, and in each test zone, the cultured fish of Example 2, Example 3, Example 4 and Comparative Example 3 The feed was fed 10 times per 1 kg (total 10 kg) for 2 weeks.

また、飼育試験3では、水揚げ時の魚体重が約2kgとなるマダイを25尾ずつ4つの試験区に分け、各試験区において、実施例5、実施例6、実施例7、比較例3の養殖魚用飼料を2週間にわたって、1kgずつ10回(合計10kg)給餌した。   Also, in breeding test 3, red sea bream with a fish weight of about 2 kg when landing is divided into 4 test sections of 25 fish, and in each test section, Example 5, Example 6, Example 7, and Comparative Example 3 Cultured fish feed was fed 10 times per kg (total 10 kg) over 2 weeks.

図3は、飼育試験2の試験結果を示しており、図3(a)は、各試験区における給餌開始から2週間経過後の各試験区においてサンプリングしたブリの筋肉中における伊予柑果皮油の主成分(D−リモネン)の含有量を分析(HS-GC-MS)した結果を示しており、図3(b)は、同じくサンプリングしたブリの官能試験結果を示している。   Fig. 3 shows the test results of breeding test 2, and Fig. 3 (a) shows the Iyo citrus peel oil in the yellowtail muscle sampled in each test group after two weeks from the start of feeding in each test group. The result of analyzing the content of the main component (D-limonene) (HS-GC-MS) is shown, and FIG. 3B shows the sensory test result of the sampled yellowtail.

図4は、飼育試験3の試験結果を示しており、図4(a)は、給餌開始から2週間経過後の各試験区においてサンプリングしたマダイの筋肉中における伊予柑果皮油の主成分(D−リモネン)の含有量を分析(HS-GC-MS)した結果を示しており、図4(b)は、同じくサンプリングしたマダイの官能試験結果を示している。   FIG. 4 shows the test results of breeding test 3, and FIG. 4 (a) shows the main component of Iyo citrus peel oil (D) in the muscles of red sea bream sampled in each test section 2 weeks after the start of feeding. -The result of analyzing the content of (limonene) (HS-GC-MS) is shown, and Fig. 4 (b) shows the sensory test result of red sea bream sampled.

なお、図3(b)及び図4(b)に結果を示した官能試験では、20代男性、30代男性、30代女性、40代男性、40代女性、50代男性、50代女性、各一人ずつの合計7人に各試験区の水揚げしたブリ、マダイの魚肉を生で食してもらい、伊予柑果皮油の香りを感じたかどうか5段階(1:感じない、2:弱く感じる、3:普通に感じる、4:強く感じる、5:強すぎる)で回答してもらった。   In the sensory test shown in FIG. 3 (b) and FIG. 4 (b), the males in their 20s, males in their 30s, females in their 30s, males in their 40s, females in their 40s, females in their 50s, A total of 7 people, one each, eats freshly caught yellowtail and red sea bream fish from each test area, and whether or not the scent of Iyo citrus peel oil is felt (1: not felt, 2: feel weak, 3 : Feel normal, 4: Feel strong, 5: Too strong).

図3(a)及び図4(a)に示すように、伊予柑果皮油を添加していない比較例3の試験区では、水揚げされた養殖魚の筋肉に伊予柑果皮油の主成分が含まれていないのに対して、伊予柑果皮油を所定量添加した実施例2〜4の試験区では、添加量に比例した量のD−リモネンが筋肉中に含まれており、飼料に添加された伊予柑果皮油が養殖魚の筋肉に良好に移行している。   As shown in FIG. 3 (a) and FIG. 4 (a), in the test area of Comparative Example 3 to which Iyo citrus peel oil was not added, the main component of Iyo citrus peel oil was contained in the muscles of the fished cultured fish. In contrast, in the test sections of Examples 2 to 4 in which a predetermined amount of Iyo citrus peel oil was added, an amount of D-limonene proportional to the amount added was contained in the muscle and added to the feed. Iyo citrus peel oil is well transferred to the muscles of cultured fish.

図3(b)に示すように、飼育試験2では、実施例3,4の養殖魚用飼料を与えた試験区のブリについて、全員が伊予柑の香りを普通以上に感じている。また、図4(b)に示すように、飼育試験3では、実施例6,7の養殖魚用飼料を与えた試験区のマダイについて、全員が伊予柑の香りを普通以上に感じている。   As shown in FIG.3 (b), in the breeding test 2, about the yellowtail of the test section which supplied the feed for cultured fish of Example 3, 4, all feel the scent of Iyokan more than usual. Moreover, as shown in FIG.4 (b), in the breeding test 3, about the red sea bream of the test area which gave the feed for cultured fish of Example 6, 7, all feel the scent of Iyokan more than usual.

このように、図3及び図4に示す結果から、養殖魚用飼料に伊予柑果皮油を添加すると、養殖魚の筋肉に伊予柑果皮油の成分が良好に移行し、所定量以上の伊予柑果皮油を養殖魚に与えることで、養殖魚の魚肉から伊予柑の香りを感じることができることが分かる。よって、本実施形態に係る養殖魚用飼料を用いることで、魚の臭みを低減させ、食する際に伊予柑の香りの漂う養殖魚を提供することができる。   Thus, from the results shown in FIG. 3 and FIG. 4, when Iyo citrus peel oil is added to the feed for cultured fish, the components of Iyo citrus peel oil migrate well to the muscles of the cultured fish, and a predetermined amount or more of Iyo citrus peel It can be seen that by giving oil to the cultured fish, the scent of Iyokan can be felt from the fish meat of the cultured fish. Therefore, by using the cultured fish feed according to the present embodiment, it is possible to reduce the smell of the fish and provide a cultured fish with a scent of Iyokan when eating.

続いて、養殖魚用飼料に添加する伊予柑果皮油の適量について検討する。上述した飼育試験結果2,3によれば、飼料に加える伊予柑果皮油の量が多くなるほど、養殖魚に移行する伊予柑の香りも強くなっている。以下、養殖魚の魚肉から伊予柑の香りがするようにするためには、伊予柑果皮油をどれくらいの量、養殖魚に与えれば良いかを検討する。   Subsequently, the appropriate amount of Iyo citrus peel oil to be added to cultured fish feed will be examined. According to the breeding test results 2 and 3 described above, as the amount of Iyo citrus peel oil added to the feed increases, the scent of Iyokan migrating to cultured fish becomes stronger. In the following, we will examine how much Iyokan peel oil should be given to the cultured fish in order to make the Iyokan fragrance from the fish meat of the cultured fish.

図5は、上記各飼育試験1〜3において、養殖魚用飼料に混合することで、最終的に養殖魚に与えられた伊予柑果皮油の総給餌量を示した図であり、養殖魚の水揚げ時の魚体重1kgあたりの総給餌量で表している。   FIG. 5 is a diagram showing the total amount of Iyo citrus peel oil fed to the cultured fish by mixing with the cultured fish feed in each of the breeding tests 1 to 3, and landing the cultured fish. Expressed as the total amount of food per kg of fish weight per hour.

まず、飼育試験1において、給餌期間が2週間(伊予柑成分の分析時)の場合、給餌1回分の1kgの養殖魚用飼料には、2gの伊予柑果皮油が含まれ、合計10回の給餌で、20gの果皮油が養殖魚に与えられる。ここで、試験区には、水揚げ時5kgのブリが10尾含まれており、水揚げ時の総魚体重は50kgである。よって、飼育試験1の10回給餌を行った時点での果皮油総給餌量は、魚体重1kgあたり、0.4gとなる。   First, in the breeding test 1, when the feeding period is 2 weeks (when analyzing the Iyokan ingredients), 1 kg of cultured fish feed for each feeding contains 2 g of Iyokan peel oil for a total of 10 times By feeding, 20g of peel oil is given to farmed fish. Here, the test zone contains 10 5kg yellowtails when landing, and the total fish weight when landing is 50kg. Therefore, the total amount of peel oil at the time of feeding 10 times in the breeding test 1 is 0.4 g per kg of fish body weight.

また、飼育試験1において、給餌期間が8週間(平均魚体重の測定)の場合、すなわち、飼育試験1の40回給餌を行った時点での果皮油総給餌量は、魚体重1kgあたり、1.6gとなる。   In the breeding test 1, when the feeding period is 8 weeks (measuring the average fish weight), that is, when the 40 feedings of the breeding test 1 are performed, the total peel oil supply is 1.6 per kg of fish body weight. g.

同じように、飼育試験2の実施例2〜4について、給餌完了時(給餌回数10回)の果皮油総給餌量は、魚体重1kgあたり、0.04g(実施例2)、0.08g(実施例3)、0.12g(実施例4)となる。また、飼育試験3の実施例5〜7について、給餌完了時(給餌回数10回)の果皮油総給餌量は、魚体重1kgあたり、0.2g(実施例5)、0.4g(実施例6)、0.6g(実施例7)となる。   Similarly, for Examples 2 to 4 of Breeding Test 2, the total amount of peel oil at the completion of feeding (10 times of feeding) is 0.04 g (Example 2) and 0.08 g (Example) per kg of fish body weight. 3), 0.12 g (Example 4). Moreover, about Examples 5-7 of breeding test 3, the total amount of peel oil at the time of completion of feeding (10 times of feeding) is 0.2 g (Example 5) and 0.4 g (Example 6) per 1 kg of fish body weight. 0.6 g (Example 7).

ここで、上述した飼育試験2についての官能試験において、実施例2(0.04g/1kg)では、伊予柑の香りを感じない人が7名中2名いるのに対して、実施例3(0.08g/1kg)では、7名全員が伊予柑の香りを感じている。よって、ブリの飼育に伊予柑果皮油入りの養殖魚用飼料を用いる場合には、水揚げ時の魚体重1kgあたり、合計で0.04g以上の伊予柑果皮油を与えるのが望ましく、さらに望ましくは、0.08g以上とするのが望ましい。   Here, in the sensory test for the breeding test 2 described above, in Example 2 (0.04 g / 1 kg), there are 2 out of 7 people who do not feel the scent of Iyokan, while Example 3 (0.08 In g / 1kg), all seven people feel the scent of Iyokan. Therefore, when using cultured fish feed containing Iyo citrus peel oil for breeding yellowtail, it is desirable to give a total of 0.04 g or more of Iyo citrus peel oil per kg of fish weight at the time of landing, more preferably Desirably 0.08g or more.

また、上述した飼育試験3についての官能試験において、実施例5(0.2g/1kg)では、伊予柑の香りを感じない人が7名中4名いるのに対して、実施例6(0.4g/1kg)では、7名全員が伊予柑の香りを感じている。よって、マダイの飼育に伊予柑果皮油入りの養殖魚用飼料を用いる場合には、水揚げ時の魚体重1kgあたり、合計で0.2g以上の養殖魚用飼料を与えるのが望ましく、さらに望ましくは、0.4g以上とするのが望ましい。   In the sensory test for breeding test 3 described above, in Example 5 (0.2 g / 1 kg), there were 4 out of 7 people who did not feel the scent of Iyokan, whereas Example 6 (0.4 g / 1kg), all 7 people feel the scent of Iyokan. Therefore, when using cultured fish feed containing Iyo citrus peel oil for raising red sea bream, it is desirable to give a total of 0.2 g or more of cultured fish feed per 1 kg of fish weight at the time of landing, Desirably 0.4 g or more.

このように、ブリよりもマダイのほうが多くの果皮油を与える必要があるのは、飼料の添加された伊予柑果皮油が養殖魚の魚肉に移行する際の効率は、養殖魚の脂肪含量に比例すると考えられるからである。マダイよりもブリのほうが脂肪含量が多いため、マダイと比べてブリのほうが少ない量の伊予柑果皮油で効率的に香り付けを行うことができる。   In this way, red sea bream needs to give more peel oil than yellowtail because the efficiency of Iyo citrus peel oil added with feed to the fish meat of the cultured fish is proportional to the fat content of the cultured fish. It is possible. Since yellowtail has a higher fat content than red sea bream, it can be efficiently scented with a small amount of yellowtail oil compared to red sea bream.

なお、伊予柑果皮油を添加した養殖魚用飼料を給餌するタイミングについて、水揚げよりも1年以上前に与えたのでは、養殖魚の魚肉に移行した伊予柑果皮油がその後の成長過程で失われてしまうおそれもあるため、水揚げの直前である必要があり、水揚げ直前の三ヶ月以内、より望ましくは二ヶ月以内に与えるのが望ましい。   In addition, if the feed for cultured fish supplemented with Iyo citrus peel oil was given more than one year before landing, Iyo citrus peel oil that had been transferred to the fish of the cultured fish was lost during the subsequent growth process. Therefore, it is necessary to give it within 3 months, more preferably within 2 months immediately before landing.

また、伊予柑果皮油を添加した養殖魚用飼料を給餌する回数は、少なくとも5日以上に分けて与えるのが望ましく、望ましくは、14日(2週間)以上に分けて与えるのが望ましい。1回の給餌では、食べる魚と食べない魚が出てくるので、個体による給餌量のばらつき、すなわち伊予柑果皮油の摂取量のばらつきを抑えるため、5日以上に分けて与える必要がある。   Moreover, the number of times of feeding the cultured fish feed to which Iyo citrus peel oil is added is preferably divided into at least 5 days, and desirably divided into 14 days (2 weeks) or more. In one feeding, fish that eat and fish that do not eat come out, so it is necessary to divide them into five or more days in order to suppress variations in the amount of food fed by individuals, that is, variations in the intake of Iyo citrus peel oil.

また、伊予柑果皮油の給餌量の上限について、果皮であれば、混ぜる果皮の量が増えると相対的に配合飼料等の量が少なくなって養殖魚の成長が阻害されてしまうため、混ぜることのできる量に限界がある。これに対して、粗精製した伊予柑果皮油であれば、香りが強くなりすぎて養殖魚が食べなくなる場合を除いて、所望の量の伊予柑果皮油を混ぜることができる。   In addition, regarding the upper limit of the amount of Iyo citrus peel oil to be fed, if it is pericarp, increasing the amount of pericarp to mix will reduce the amount of compounded feed and the like, and the growth of cultured fish will be hindered. There is a limit to the amount that can be achieved. On the other hand, if it is crudely refined Iyo citrus peel oil, a desired amount of Iyo citrus peel oil can be mixed except when the scent becomes too strong to stop eating cultured fish.

以上、本実施形態について詳細に説明したが、本実施形態に係る伊予柑果皮油を添加した養殖魚用飼料によれば、蒸留することなく粗精製した伊予柑果皮油を添加することで、養殖魚の成長を阻害することなく、養殖魚の魚肉に伊予柑の香り付けをし、魚臭さを低減させることができる。これにより、高品質の養殖魚を養殖し、養殖業者の売上向上に貢献することができる。   As mentioned above, although this embodiment was described in detail, according to the feed for cultured fish which added the Iyo citrus peel oil which concerns on this embodiment, by adding the Iyo citrus peel oil refine | purified roughly without distilling, aquaculture Without inhibiting the growth of fish, the fish meat of cultured fish can be scented with Iyokan to reduce the smell of fish. Thereby, it is possible to cultivate high-quality cultured fish and contribute to the improvement of the sales of the aquaculture company.

また、本実施形態では、粗精製した伊予柑果皮油を飼料に混ぜており、果皮ごと混ぜる場合と違って、モイストペレットだけでなく、ドライペレットであっても容易に伊予柑果皮油を混ぜることができる。   Moreover, in this embodiment, roughly refined Iyo citrus peel oil is mixed with the feed, and unlike the case of mixing the whole peel, Iyo citrus peel oil can be easily mixed with not only moist pellets but also dry pellets. Can do.

以上、本発明の実施の形態について説明したが、本発明の実施の形態は、上記実施形態に限定されるものではなく、本発明の主旨を逸脱しない範囲内で種々の変形が可能である。例えば、養殖魚用飼料の配合成分や配合比は適宜変更可能であり、養殖魚用飼料を与えるタイミングや量、回数等も適宜変更可能である。   Although the embodiments of the present invention have been described above, the embodiments of the present invention are not limited to the above-described embodiments, and various modifications can be made without departing from the gist of the present invention. For example, the composition component and composition ratio of the feed for cultured fish can be changed as appropriate, and the timing, amount, number of times, and the like of giving the feed for cultured fish can be changed as appropriate.

また、上記実施形態では、養殖魚として、ブリやマダイの場合を例に挙げて説明したが、カンパチやマグロ等、適宜他の魚種に本発明を適用することができる。この場合、給餌する柑橘果皮油の量は、魚種の脂肪含量に合わせて適宜調整するのが望ましい。   Moreover, in the said embodiment, although the case of a yellowtail and a red sea bream was mentioned as an example as a cultured fish, it demonstrated and this invention is applicable to other fish species, such as amberjack and a tuna. In this case, it is desirable that the amount of citrus peel oil to be fed is appropriately adjusted according to the fat content of the fish species.

また、上記実施形態では、柑橘として伊予柑を使用し、養殖魚用飼料に伊予柑果皮油を添加したが、柑橘としては、温州みかん、ゆず、オレンジ、グレープフルーツ等、他の柑橘を適宜使用することができ、これらの果皮から採取した果皮油を飼料に添加すれば良い。   Moreover, in the said embodiment, Iyokan was used as citrus, and Iyo citrus peel oil was added to the feed for cultured fish, but other citrus such as Wenzhou mandarin orange, citron, orange, grapefruit, etc. are used as appropriate. The peel oil collected from these peels may be added to the feed.

Claims (6)

圧搾法により柑橘の果皮から採取して、蒸留することなく粗精製した果皮油を添加したことを特徴とする養殖魚用飼料。   A feed for cultured fish, characterized in that it is extracted from the peel of citrus fruits by a pressing method, and crudely purified peel oil is added without distillation. 圧搾法により柑橘の果皮から採取して、蒸留することなく粗精製した果皮油を添加した養殖魚用飼料を、養殖魚に所定量与える果皮油給餌工程を備えることを特徴とする養殖魚の養殖方法。   A method for cultivating cultured fish, comprising a step of feeding the cultured fish with a predetermined amount of cultured fish feed that has been extracted from citrus peel by pressing and added with crude oil that has been roughly purified without distillation . 前記果皮油給餌工程は、前記養殖魚の水揚げ直前の三ヶ月以内に行われる工程であることを特徴とする請求項2記載の養殖魚の養殖方法。   The method for culturing cultured fish according to claim 2, wherein the skin oil feeding step is a step performed within three months immediately before landing of the cultured fish. 前記果皮油給餌工程は、少なくとも5日以上に分けて与える工程であることを特徴とする請求項2又は3記載の養殖魚の養殖方法。   The method for culturing cultured fish according to claim 2 or 3, wherein the skin oil feeding step is a step of giving at least 5 days. 前記養殖魚はブリであり、
前記果皮油給餌工程は、水揚げ時の魚体重1kgあたり合計0.04g以上の伊予柑果皮油を前記ブリに与える工程であることを特徴とする請求項2乃至4何れか1項記載の養殖魚の養殖方法。
The farmed fish is yellowtail,
The cultured fish according to any one of claims 2 to 4, wherein the pericarp oil feeding step is a step of giving 0.04 g or more of Iyo citrus pericarp oil to the yellowtail per 1 kg of fish weight at the time of landing. Method.
前記養殖魚はマダイであり、
前記果皮油給餌工程は、水揚げ時の魚体重1kgあたり合計0.2g以上の伊予柑果皮油を前記マダイに与える工程であることを特徴とする請求項2乃至4何れか1項記載の養殖魚の養殖方法。
The farmed fish is red sea bream,
The cultured fish according to any one of claims 2 to 4, wherein the pericarp oil feeding step is a step of providing the red sea bream with a total of 0.2 g or more of Iyo citrus pericarp oil per kg of fish weight at the time of landing. Method.
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