JP5662178B2 - Antioxidant composition - Google Patents
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- JP5662178B2 JP5662178B2 JP2011014001A JP2011014001A JP5662178B2 JP 5662178 B2 JP5662178 B2 JP 5662178B2 JP 2011014001 A JP2011014001 A JP 2011014001A JP 2011014001 A JP2011014001 A JP 2011014001A JP 5662178 B2 JP5662178 B2 JP 5662178B2
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Description
本発明は抗酸化組成物に関するものであり、詳細には、植物油蒸留留出物を有効成分として含有する抗酸化組成物に関するものである。 The present invention relates to an antioxidant composition, and more particularly to an antioxidant composition containing a vegetable oil distillate as an active ingredient.
世界中で広く使用されている飼料用抗酸化剤として、エトキシキン（6-ethoxy-1,2-dihydro-2,2,4-trimethylquinoline）やＢＨＴ（butylated hydroxytoluen）が知られている。エトキシキンおよびＢＨＴは、非常に強い抗酸化能を有する合成抗酸化剤であるが、近年、安全性の観点からその使用を疑問視する声が広まっている。しかし、エトキシキンやＢＨＴと同等の抗酸化能を有し、安全かつ安価な抗酸化剤は存在しない。そのため、エトキシキンやＢＨＴに代わる飼料用抗酸化剤の開発が、食糧の安定供給の観点から非常に重要な課題となっている。 As feed antioxidants widely used all over the world, ethoxyquin (6-ethoxy-1,2-dihydro-2,2,4-trimethylquinoline) and BHT (butylated hydroxytoluen) are known. Ethoxyquin and BHT are synthetic antioxidants having very strong antioxidant ability, but in recent years, there are widespread voices questioning their use from the viewpoint of safety. However, there is no safe and inexpensive antioxidant that has the same antioxidant ability as ethoxyquin and BHT. Therefore, development of feed antioxidants that replace ethoxyquin and BHT has become a very important issue from the viewpoint of stable food supply.
一方、食品用抗酸化剤としては、ビタミンＣ、ビタミンＥなどのビタミン系抗酸化剤が広く使用されている。しかしながら、ビタミン系抗酸化剤は合成品が多いことから、抗酸化能を有する天然の抗酸化組成物の開発が望まれている。 On the other hand, vitamin antioxidants such as vitamin C and vitamin E are widely used as food antioxidants. However, since many vitamin-based antioxidants are synthetic products, it is desired to develop a natural antioxidant composition having antioxidant ability.
植物油の精製工程における脱臭工程等の蒸留工程で多量に排出される蒸留留出物には、植物ステロール、ビタミンＥ類、スクワレンなどの不けん化物が含まれていることが知られているが、その含有量が低いため有効利用されておらず廃棄物扱いとなっている。植物油蒸留留出物の利用としては、例えば特許文献１には、植物油蒸留留出物（脱臭スカム油）を飼料に添加して家畜や家禽に与えることにより、畜産物の低コレステロール化を図ることが記載されている。特許文献２には、米糠油抽出物（米油脱臭留出物）を飼料に添加して家禽に与えることにより、トコトリエノール含量が増加した家禽卵が得られることが記載されている。しかし、植物油蒸留留出物が上記公知の抗酸化剤に匹敵する抗酸化能を有していることは知られていなかった。 It is known that the distillate discharged in a large amount in the distillation process such as the deodorization process in the refining process of vegetable oil contains unsaponifiable substances such as plant sterols, vitamin Es, and squalene. Since its content is low, it is not used effectively and is treated as waste. For example, Patent Document 1 discloses that a vegetable oil distilled distillate (deodorized scum oil) is added to feed and given to livestock and poultry to lower the cholesterol of livestock products. Is described. Patent Document 2 describes that poultry eggs having an increased tocotrienol content can be obtained by adding rice bran oil extract (rice oil deodorized distillate) to feed and giving it to poultry. However, it was not known that the vegetable oil distillate has an antioxidant capacity comparable to the above known antioxidants.
本発明は、エトキシキンやＢＨＴに匹敵する抗酸化能を有し、安価であり、食品や化粧品にも使用できるレベルの安全性があり、家畜や家禽の成長に影響を及ぼさない抗酸化組成物を提供することを目的とする。 The present invention provides an antioxidant composition that has an antioxidant capacity comparable to ethoxyquin and BHT, is inexpensive, has a level of safety that can be used for food and cosmetics, and does not affect the growth of livestock and poultry. The purpose is to provide.
本発明は、上記課題を解決するために、以下の各発明を包含する。
［１］植物油蒸留留出物を有効成分として含有することを特徴とする抗酸化組成物。
［２］植物油が米油である前記［１］に記載の抗酸化組成物。
［３］食品、飼料、化粧品または農薬用である前記［１］に記載の抗酸化組成物。
［４］前記［１］〜［３］のいずれかに記載の抗酸化組成物を食品、飼料、化粧品または農薬用に添加することを特徴とする食品、化粧品、農薬または飼料の酸化抑制方法。
［５］前記［１］〜［３］のいずれかに記載の抗酸化組成物を添加した飼料を家畜または家禽に給餌することを特徴とする食肉の酸化抑制方法。
The present invention includes the following inventions in order to solve the above problems.
[1] An antioxidant composition comprising a vegetable oil distillate as an active ingredient.
[2] The antioxidant composition according to [1], wherein the vegetable oil is rice oil.
[3] The antioxidant composition according to the above [1], which is for food, feed, cosmetics or agricultural chemicals.
[4] A method for inhibiting oxidation of foods, cosmetics, agricultural chemicals or feeds, comprising adding the antioxidant composition according to any one of [1] to [3] to foods, feeds, cosmetics or agricultural chemicals.
[5] A method for suppressing oxidation of meat, comprising feeding a livestock or poultry with a feed to which the antioxidant composition according to any one of [1] to [3] is added.
本発明によれば、エトキシキンやＢＨＴに匹敵する抗酸化能を有し、安価であり、安全性が高く、家畜や家禽の成長に影響を及ぼさない抗酸化組成物を提供することができる。本発明の抗酸化組成物を食品、化粧品、農薬または飼料に添加すれば、これらの酸化を抑制することができ、飼料に添加して家畜または家禽に給餌すれば、食肉の酸化を抑制することができる。 According to the present invention, it is possible to provide an antioxidant composition that has an antioxidant ability comparable to that of ethoxyquin and BHT, is inexpensive, has high safety, and does not affect the growth of livestock and poultry. If the antioxidant composition of the present invention is added to food, cosmetics, agricultural chemicals or feed, these oxidations can be suppressed, and if added to the feed and fed to livestock or poultry, the oxidation of meat is suppressed. Can do.
本発明は、植物油蒸留留出物を有効成分として含有する抗酸化組成物を提供する。
上述のように、植物油蒸留留出物にはビタミンＥ類が含まれていることが知られている。例えば、米油の精製工程における脱臭工程で生じる米油蒸留留出物には、約２％の総トコフェロールと約２％の総トコトリエノールが含まれており、ビタミンＥ含量は約４％である（日本畜産学会報第78巻第2号 p.179-186, 2007）。そして、ビタミンＥが抗酸化能を有することは公知である。しかしながら、本発明者らは、廃食用油に米油蒸留留出物を１００ｐｐｍ添加した場合（ビタミンＥ濃度は約４ｐｐｍ）、廃食用油にビタミンＥを１００ｐｐｍ添加した場合と同等の抗酸化能があることを見出した（実施例３参照）。つまり、本発明者らは、米油蒸留留出物には、自身に内在するビタミンＥ濃度より約２５倍高濃度のビタミンＥを単独で添加した場合と同等の抗酸化活性があることを見出し、米油蒸留留出物の抗酸化能は内在するビタミンＥのみに基づくものでないことの確信を得て、本発明に至ったものである。
The present invention provides an antioxidant composition containing a vegetable oil distillate as an active ingredient.
As described above, it is known that the vegetable oil distillation distillate contains vitamin Es. For example, the rice oil distillate produced in the deodorization process in the rice oil refining process contains about 2% total tocopherol and about 2% total tocotrienol, and has a vitamin E content of about 4% ( The Japanese Society of Animal Science Vol.78, No.2, p.179-186, 2007). And it is well-known that vitamin E has antioxidant ability. However, when the present inventors add 100 ppm of rice oil distillate to waste edible oil (concentration of vitamin E is about 4 ppm), the antioxidant capacity is the same as when 100 ppm of vitamin E is added to waste edible oil. It was found (see Example 3). That is, the present inventors have found that the rice oil distillate has an antioxidant activity equivalent to that obtained by adding vitamin E at a concentration about 25 times higher than the vitamin E concentration inherent in itself. The present invention has been obtained with the conviction that the antioxidant ability of the distillate of rice oil is not based on the inherent vitamin E alone.
植物油蒸留留出物とは、植物油の精製工程における蒸留を伴う工程で発生する留出物を意味する。蒸留方法は特に限定されないが、例えば、分子蒸留（薄膜蒸留）、水蒸気蒸留等を用いることが好ましい。蒸留を伴う工程としては、例えば脱臭工程が挙げられるが、脱臭工程で発生する留出物（「脱臭留出物」、「脱臭スカム油」と称される場合がある。）に限定されるものではない。植物油蒸留留出物は、精製工程において蒸留を伴う工程を行う植物油であれば、いずれの植物油由来の蒸留留出物も取得することができる。なかでも、米油、大豆油、コーン油、綿実油、菜種油由来の蒸留留出物が好ましく、より好ましくは米油蒸留留出物である。 The vegetable oil distillate means a distillate generated in a process involving distillation in a vegetable oil refining process. Although the distillation method is not particularly limited, for example, it is preferable to use molecular distillation (thin film distillation), steam distillation or the like. Examples of the process involving distillation include a deodorization process, but are limited to distillates generated in the deodorization process (sometimes referred to as “deodorized distillate” and “deodorized scum oil”). is not. As long as the vegetable oil distillate is a vegetable oil that undergoes a process involving distillation in the refining process, any vegetable oil-derived distillate can be obtained. Of these, a distillate derived from rice oil, soybean oil, corn oil, cottonseed oil, and rapeseed oil is preferable, and a distillate distillate from rice oil is more preferable.
植物油蒸留留出物は、植物油の精製工程における蒸留を伴う工程で発生する留出物そのものでもよく、これに対して何らかの処理を行ったものでもよい。例えば、脱酸、脱色、脱臭等の処理を行ったものも、植物油蒸留留出物に含まれる。処理の種類は限定されるものではなく、抗酸化能を有する限りにおいて、植物油の精製工程における蒸留を伴う工程で発生する植物油蒸留留出物に対してどのような処理を行ったものでもよい。 The vegetable oil distillation distillate may be a distillate itself generated in a process involving distillation in a vegetable oil refining process, or may be obtained by performing some kind of treatment on the distillate. For example, what processed deacidification, decoloring, deodorizing, etc. is also contained in a vegetable oil distillation distillate. The kind of the treatment is not limited, and any treatment may be performed on the vegetable oil distillate generated in the step involving distillation in the vegetable oil refining step as long as it has antioxidant ability.
植物油蒸留留出物は、通常、酸価が１００以下であり、不ケン化物を２０〜８０質量％程度、ビタミンＥ類（トコフェロール、トコトリエノール）を２〜８質量％程度含んでいる。脂肪酸組成は、パルミチン酸が２４〜３０質量％程度、オレイン酸が３７〜４３質量％程度、リノール酸が２６〜３２質量％程度、リノレン酸が５質量％以下である。このように、植物油蒸留留出物には有用な生理活性成分が含まれているが、その含有量が低いため有効利用されておらず廃棄物扱いとなっている。それゆえ、本発明の抗酸化組成物は、極めて安価な原料を利用できる点で非常に有利である。 The vegetable oil distillate usually has an acid value of 100 or less, contains about 20 to 80% by mass of unsaponifiable matter, and contains about 2 to 8% by mass of vitamin E (tocopherol, tocotrienol). The fatty acid composition is such that palmitic acid is about 24 to 30% by mass, oleic acid is about 37 to 43% by mass, linoleic acid is about 26 to 32% by mass, and linolenic acid is 5% by mass or less. As described above, the distillate of vegetable oil contains useful physiologically active components. However, since the content thereof is low, it is not effectively used and is treated as waste. Therefore, the antioxidant composition of the present invention is very advantageous in that an extremely inexpensive raw material can be used.
本発明の抗酸化組成物は、植物油蒸留留出物のみからなるものでもよいが、食品等に通常使用される公知の担体を用いて製剤化することができる。剤形は特に限定されないが、例えば、液剤、散剤、顆粒剤、丸剤、錠剤等が挙げられる。 The antioxidant composition of the present invention may consist only of a vegetable oil distillate, but can be formulated using a known carrier usually used for foods and the like. Although a dosage form is not specifically limited, For example, a liquid agent, a powder, a granule, a pill, a tablet etc. are mentioned.
本発明の抗酸化組成物は、例えば食品、飼料、化粧品、農薬等に添加することにより、これらの酸化による劣化を抑制することができる。特に、本発明の抗酸化組成物は、植物油の精製過程で副生される植物油蒸留留出物を有効成分とするものであるため安全性に問題がなく、かつ極めて安価に製造できるので、エトキシキンやＢＨＴに代わる飼料用抗酸化組成物として非常に有用である。本発明の抗酸化組成物を添加することによる酸化抑制効果は、例えば、本発明の抗酸化組成物を添加したサンプルと添加していなサンプルについて、酸価または過酸化物価を測定し、比較することによって確認することができる。酸価の測定および過酸化物価の測定は、例えば、基準油脂分析試験法、飼料分析法等の公知の方法を用いて行うことができる。 By adding the antioxidant composition of the present invention to, for example, foods, feeds, cosmetics, agricultural chemicals, and the like, deterioration due to such oxidation can be suppressed. In particular, since the antioxidant composition of the present invention contains a vegetable oil distillate produced as a by-product in the process of refining vegetable oil as an active ingredient, it has no safety problem and can be produced at a very low cost. It is very useful as an antioxidant composition for feed that replaces BHT and BHT. The effect of inhibiting oxidation by adding the antioxidant composition of the present invention is measured, for example, by comparing the acid value or peroxide value of the sample to which the antioxidant composition of the present invention is added and the sample to which the antioxidant composition is not added. Can be confirmed. The acid value and the peroxide value can be measured using a known method such as a standard fat analysis method or a feed analysis method.
本発明の抗酸化組成物を添加する食品は、特に限定されないが、例えば、茶飲料、清涼飲料、炭酸飲料、栄養飲料、果実飲料、乳酸飲料等の飲料、そば、うどん、中華麺、即席麺等の麺類、飴、キャンディー、ガム、チョコレート、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼き菓子、パン等の菓子およびパン類、かまぼこ、ハム、ソーセージ等の水産・畜産加工食品、加工乳、発酵乳等の乳製品、サラダ油、てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ドレッシング等の油脂および油脂加工食品、ソース、たれ等の調味料、カレー、シチュー、丼、お粥、雑炊等のレトルトパウチ食品などを挙げることができる。 The food to which the antioxidant composition of the present invention is added is not particularly limited. For example, beverages such as tea drinks, soft drinks, carbonated drinks, nutrition drinks, fruit drinks, and lactic acid drinks, buckwheat, udon, Chinese noodles, and instant noodles Noodles such as candy, candy, gum, chocolate, snacks, biscuits, jelly, jam, cream, baked goods, bread and other confectionery and breads, fishery products such as kamaboko, ham, sausage, processed foods, processed milk, fermentation Dairy products such as milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and other fats and oils processed foods, sauces, sauces and other seasonings, curry, stew, rice cakes, rice cakes, miscellaneous cooking retort Examples include pouch foods.
本発明の抗酸化組成物を添加する飼料は、特に限定されないが、例えば、ウシ、ウマ、ブタ等の家畜用飼料、ニワトリ等の家禽用飼料、イヌ、ネコ等のペット用飼料、養殖魚用飼料などが挙げられる。 The feed to which the antioxidant composition of the present invention is added is not particularly limited. For example, livestock feed such as cattle, horses and pigs, poultry feed such as chickens, pet feed such as dogs and cats, and cultured fish. For example, feed.
本発明の抗酸化組成物を、食品、飼料、化粧品または農薬に添加する場合、その添加量は特に限定されないが、食品、化粧品または農薬に添加する場合は、通常、約０．００５〜０．０５質量％であり、好ましくは約０．０１〜０．０３質量％である。飼料に添加する場合は、通常、約０．２５〜５．０質量％であり、好ましくは約０．５〜３．０質量％である。 When the antioxidant composition of the present invention is added to foods, feeds, cosmetics or agricultural chemicals, the addition amount is not particularly limited, but when added to foods, cosmetics or agricultural chemicals, it is usually about 0.005 to 0.00. It is 05 mass%, Preferably it is about 0.01-0.03 mass%. When adding to feed, it is about 0.25-5.0 mass% normally, Preferably it is about 0.5-3.0 mass%.
本発明者らは、本発明の抗酸化組成物を添加した飼料を鶏に給餌することにより、当該飼料を摂取した鶏から採取した食肉の酸化を抑制できることを見出した。したがって、本発明は、上記本発明の抗酸化組成物を添加した飼料を家畜または家禽に給餌することを特徴とする食肉の酸化抑制方法を提供する。本発明の食肉の酸化抑制方法は、植物油蒸留留出物を添加した飼料を家畜または家禽に給餌する工程を含むものであればよく、通常、家畜または家禽から食肉を採取する工程をさらに含む。 The present inventors have found that by feeding a chicken with a feed to which the antioxidant composition of the present invention has been added, the oxidation of meat collected from the chicken that has taken the feed can be suppressed. Therefore, the present invention provides a method for inhibiting oxidation of meat, characterized in that the feed containing the antioxidant composition of the present invention is fed to livestock or poultry. The method for suppressing oxidation of meat according to the present invention only needs to include a step of feeding livestock or poultry with feed supplemented with vegetable oil distillate, and usually further includes a step of collecting meat from livestock or poultry.
植物油蒸留留出物を添加した飼料を家畜または家禽に給餌する期間は特に限定されないが、少なくとも食肉採取前７日間以上給餌することが好ましく、１４日間以上給餌することがより好ましい。最も好ましくは、全飼育期間を通して給餌することである。
食肉の酸化が抑制されていることは、例えば、本発明の抗酸化組成物を添加した飼料を給餌した個体から採取した食肉と、本発明の抗酸化組成物を添加していない飼料を給餌した個体から採取した食肉について、一定期間経過後の過酸化物価を測定し、比較することによって確認することができる。過酸化物価の測定は、例えば、基準油脂分析試験法、飼料分析法等の公知の方法を用いて行うことができる。
The period for feeding the feed to which the vegetable oil distillate is added to livestock or poultry is not particularly limited, but it is preferably at least 7 days before meat collection, more preferably 14 days or more. Most preferred is feeding throughout the entire breeding period.
The fact that the oxidation of the meat is suppressed means, for example, that the meat collected from an individual fed with the feed added with the antioxidant composition of the present invention and the feed not added with the antioxidant composition of the present invention were fed. It can be confirmed by measuring and comparing the peroxide value after a certain period of time for meat collected from individuals. The peroxide value can be measured using, for example, a known method such as a standard fat analysis method or a feed analysis method.
以下、実施例により本発明を詳細に説明するが、本発明はこれらに限定されるものではない。 EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited to these.
〔実施例１：米油蒸留留出物の取得ならびに性状と成分組成〕
米油の精製工程における脱臭工程で発生した米油蒸留留出物を取得した。その性状および成分組成は以下の通りであった。
＜酸価、不けん化物＞
酸価 ７７．２（基準油脂分析試験法）
不けん化物 ２８．５（基準油脂分析試験法）
＜ビタミンＥ類＞
総トコフェロール １．９質量％（ＨＰＬＣ法）
総トコトリエノール ２．１質量％（ＨＰＬＣ法）
＜脂肪酸組成＞
パルミチン酸（Ｃ１６：０） ２７質量％（ＧＣ法）
ステアリン酸（Ｃ１８：０） ２質量％（ＧＣ法）
オレイン酸 （Ｃ１８：１） ４０質量％（ＧＣ法）
リノール酸 （Ｃ１８：２） ２９質量％（ＧＣ法）
リノレン酸 （Ｃ１８：３） ２質量％（ＧＣ法）
[Example 1: Acquisition and properties and composition of rice oil distillate]
The rice oil distillation distillate generated in the deodorizing step in the rice oil refining step was obtained. Its properties and component composition were as follows.
<Acid value, unsaponifiable matter>
Acid value 77.2 (standard oil and fat analysis test method)
Unsaponifiable matter 28.5 (standard oil and fat analysis test method)
<Vitamin E>
Total tocopherol 1.9% by mass (HPLC method)
Total tocotrienol 2.1% by mass (HPLC method)
<Fatty acid composition>
Palmitic acid (C16: 0) 27% by mass (GC method)
Stearic acid (C18: 0) 2% by mass (GC method)
Oleic acid (C18: 1) 40% by mass (GC method)
Linoleic acid (C18: 2) 29% by mass (GC method)
Linolenic acid (C18: 3) 2% by mass (GC method)
〔実施例２：植物油蒸留留出物のパーム油に対する抗酸化能の検討〕
（１）材料
油脂として、パーム油を使用した。植物油蒸留留出物として、米油の精製工程における脱臭工程で発生した蒸留留出物を使用した。既知の抗酸化剤として、エトキシキンおよびＢＨＴを使用した。
[Example 2: Examination of antioxidant ability of vegetable oil distillate to palm oil]
(1) Material Palm oil was used as fats and oils. As the vegetable oil distillate, the distillate generated in the deodorizing step in the rice oil refining step was used. Ethoxyquin and BHT were used as known antioxidants.
（２）試験方法
以下の試験区を設定し、酸価および過酸化物価を測定した。
試験区１：パーム油
試験区２：パーム油＋エトキシキン １００ｐｐｍ
試験区３：パーム油＋ＢＨＴ １００ｐｐｍ
試験区４：パーム油＋米油蒸留留出物 １００ｐｐｍ
各試料調製後、ガラス瓶に分注し、６０℃で遮光保存し、保存０週目（調製直後）および９週目に酸価、過酸化物価の測定を実施した。酸価、過酸化物価の測定は「飼料分析法・解説２００４（飼料分析基準研究会編著）」に記載の方法に準じて行った。
(2) Test method The following test sections were set, and the acid value and peroxide value were measured.
Test Zone 1: Palm Oil Test Zone 2: Palm Oil + Ethoxyquin 100ppm
Test Zone 3: Palm oil + BHT 100ppm
Test section 4: Palm oil + rice oil distillate 100ppm
After each sample was prepared, it was dispensed into a glass bottle and stored in a shade at 60 ° C., and acid value and peroxide value were measured at 0 weeks (immediately after preparation) and 9 weeks of storage. The acid value and the peroxide value were measured according to the method described in “Feed Analysis Method / Comment 2004 (edited by the Feed Analysis Standards Study Group)”.
酸価の測定結果を図１に示した。図１から明らかなように、９週目において、米油蒸留留出物を添加した試験区４は、試験区１と比較して有意に酸価が減少した。試験区４の酸価減少の程度は、エトキシキン（試験区２）およびＢＨＴ（試験区３）と同等であった。この結果から、米油蒸留留出物は、エトキシキンおよびＢＨＴと同程度の抗酸化能を有することが明らかとなった。 The measurement result of the acid value is shown in FIG. As is clear from FIG. 1, in the 9th week, the test group 4 to which the rice oil distillate was added had a significantly reduced acid value compared to the test group 1. The degree of acid value reduction in test group 4 was equivalent to that of ethoxyquin (test group 2) and BHT (test group 3). From this result, it was clarified that the rice oil distillate has an antioxidant capacity comparable to that of ethoxyquin and BHT.
過酸化物価の測定結果を図２に示した。図２から明らかなように、９週目において、米油蒸留留出物を添加した試験区４は、試験区１と比較して有意に過酸化物価の上昇を抑制した。一方、エトキシキンを添加した試験区２は、試験区１より過酸化物価を上昇させ、ＢＨＴを添加した試験区３は、試験区１と差がなかった。この結果から、米油蒸留留出物はエトキシキンおよびＢＨＴより優れた過酸化物価上昇抑制効果、すなわち抗酸化能を有することが明らかとなった。 The measurement results of the peroxide value are shown in FIG. As apparent from FIG. 2, the test group 4 to which the rice oil distillate was added at the 9th week significantly suppressed the increase in the peroxide value as compared with the test group 1. On the other hand, test group 2 to which ethoxyquin was added increased the peroxide value from test group 1, and test group 3 to which BHT was added had no difference from test group 1. From this result, it was clarified that the rice oil distillate has an excellent inhibitory effect on increase in the peroxide value, that is, antioxidant ability, superior to ethoxyquin and BHT.
〔実施例３：植物油蒸留留出物およびビタミンＥの抗酸化能の比較〕
（１）材料
廃食用油を使用した。植物油蒸留留出物として、米油の精製工程における脱臭工程で発生した蒸留留出物を使用した。ビタミンＥには、飼料用ビタミンＥを使用した。既知の抗酸化剤として、エトキシキンを使用した。
[Example 3: Comparison of antioxidant capacity of vegetable oil distillate and vitamin E]
(1) Material Waste cooking oil was used. As the vegetable oil distillate, the distillate generated in the deodorizing step in the rice oil refining step was used. For vitamin E, vitamin E for feed was used. Ethoxyquin was used as a known antioxidant.
（２）試験方法
以下の試験区を設定し、ＣＤＭ試験を実施した。
試験区１：廃食用油
試験区２：廃食用油＋エトキシキン １００ｐｐｍ
試験区３：廃食用油＋ビタミンＥ １００ｐｐｍ
試験区４：廃食用油＋米油蒸留留出物 １００ｐｐｍ
ＣＤＭ試験は、「２００３年版基準油脂分析試験法（社団法人日本油化学会編纂）」に記載の方法に従って実施した。具体的には、自動油脂安定性試験装置ランシマット７４３型（メトロームジャパン株式会社、日本油化学会制定基準油脂分析試験法２００３年版のＣＤＭ試験に準拠）を用い、１１０℃の条件で、ＣＤＭ試験を実施した。
(2) Test method The following test plots were set and a CDM test was performed.
Test Zone 1: Waste cooking oil Test Zone 2: Waste cooking oil + ethoxyquin 100ppm
Test Zone 3: Waste cooking oil + Vitamin E 100ppm
Test section 4: Waste cooking oil + rice oil distillate 100ppm
The CDM test was performed according to the method described in “2003 Standard Oil and Fat Analysis Test Method (edited by Japan Oil Chemists' Society)”. Specifically, using an automatic oil stability test apparatus Rancimat 743 type (Metrohm Japan Co., Ltd., based on the Japan Oil Chemical Society established standard oil analysis method 2003 version CDM test), the CDM test was performed at 110 ° C. Carried out.
結果を図３に示した。図３から明らかなように、米油蒸留留出物を添加した試験区４は、試験区１と比較して、ＣＤＭ値の低下を有意に抑制した。試験区４のＣＤＭ値の低下抑制の程度は、エトキシキン（試験区２）およびビタミンＥ（試験区３）と同等であり、米油蒸留留出物は、エトキシキンおよびビタミンＥと同程度の抗酸化能を有することが明らかとなった。
ここで、上述のように、米油蒸留留出物には、ビタミンＥとして、総トコフェロールおよび総トコトリエノールがそれぞれ約２％含まれている。したがって、ビタミンＥを１００ｐｐｍ添加した試験区３と、米油蒸留留出物（ビタミンＥ含量約４％）を１００ｐｐｍ添加した試験区４のビタミンＥ量を比較すると、試験区３は試験区４の約２５倍量のビタミンＥを含有していることになる。しかし、図３から明らかなように、両者の抗酸化能は同等であり、２５倍の差はなかった。したがって、米油蒸留留出物の抗酸化能がビタミンＥの抗酸化能のみに基づくものでないことが明らかとなった。
The results are shown in FIG. As is clear from FIG. 3, the test group 4 to which the rice oil distillate was added significantly suppressed the decrease in the CDM value as compared to the test group 1. The degree of suppression of the decrease in the CDM value in test group 4 is equivalent to that of ethoxyquin (test group 2) and vitamin E (test group 3), and the rice oil distilled distillate has the same level of antioxidant as ethoxyquin and vitamin E. It became clear that it had the ability.
Here, as described above, the rice oil distillate contains about 2% of total tocopherol and total tocotrienol as vitamin E, respectively. Therefore, when comparing the amount of vitamin E in test group 3 to which 100 ppm of vitamin E was added and test group 4 to which 100 ppm of rice oil distilled distillate (vitamin E content of about 4%) was added, test group 3 It contains about 25 times the amount of vitamin E. However, as is clear from FIG. 3, the antioxidant capacity of the two was the same, and there was no difference of 25 times. Therefore, it became clear that the antioxidant ability of the rice oil distillate was not based solely on the antioxidant ability of vitamin E.
〔実施例４：植物油蒸留留出物添加飼料で飼育した鶏における鶏皮オイルの酸化抑制効果の検討〕
通常の鶏用飼料に米油蒸留留出物が０．５質量％となるように添加し、２０羽の鶏に４週間給餌した。コントロールとして、米油蒸留留出物を添加していない通常の鶏用飼料を、２０羽の鶏に４週間給餌した。飼育終了後に鶏から胸肉の皮を採取した。採取した鶏皮を刻んでクロロホルム：メタノール＝１：１（ｖ／ｖ）で油分を抽出した。抽出した鶏皮オイルを５０℃で保存し、経時的にサンプリングして過酸化物価を測定した。過酸化物価の測定は、実施例１と同様の方法で行った。
[Example 4: Examination of Oxidation-Inhibiting Effect of Chicken Skin Oil in Chickens Breeded with Vegetable Oil Distillate-Added Feed]
The rice distillate distillate was added to a normal chicken feed so that the amount was 0.5% by mass, and 20 chickens were fed for 4 weeks. As a control, 20 chickens were fed for 4 weeks with normal chicken feed to which no rice oil distillate was added. After the breeding, the skin of the breast was collected from the chicken. The collected chicken skin was chopped and the oil was extracted with chloroform: methanol = 1: 1 (v / v). The extracted chicken skin oil was stored at 50 ° C. and sampled over time to measure the peroxide value. The peroxide value was measured in the same manner as in Example 1.
いずれの群も摂餌量に差はなく、死亡例もなかったことから、米油蒸留留出物の飼料への添加は鶏の成長に影響を与えず、安全面でも問題がないと認められた。
過酸化物価の測定結果を図４に示した。図４から明らかなように、米油蒸留留出物添加飼料で飼育した鶏から抽出した鶏皮オイルは、通常飼料で飼育した鶏から抽出した鶏皮オイルにおける経時的な過酸化物価の上昇を顕著に抑制した。この結果から、米油蒸留留出物添加飼料を給餌することにより、酸化安定性に優れた食肉を生産できることが明らかとなった。
There was no difference in food consumption in any group, and there were no deaths. Therefore, it was recognized that adding rice oil distillate to feed did not affect chicken growth and there was no problem in terms of safety. It was.
The measurement results of the peroxide value are shown in FIG. As is apparent from FIG. 4, chicken skin oil extracted from chickens fed with rice oil distillate-added feed increased the peroxide value over time in chicken skin oil extracted from chickens fed with normal feed. Remarkably suppressed. From this result, it was clarified that meat with excellent oxidative stability can be produced by feeding rice oil distillate-added feed.
なお本発明は上述した各実施形態および実施例に限定されるものではなく、請求項に示した範囲で種々の変更が可能であり、異なる実施形態にそれぞれ開示された技術的手段を適宜組み合わせて得られる実施形態についても本発明の技術的範囲に含まれる。また、本明細書中に記載された学術文献および特許文献の全てが、本明細書中において参考として援用される。 The present invention is not limited to the above-described embodiments and examples, and various modifications are possible within the scope shown in the claims, and technical means disclosed in different embodiments are appropriately combined. The obtained embodiment is also included in the technical scope of the present invention. Moreover, all the academic literatures and patent literatures described in this specification are incorporated herein by reference.
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| KR102207915B1 (en) * | 2020-03-17 | 2021-01-26 | 다윈그룹(주) | Manufacturing method of cosmetic composition using rice and steam distiller using the same |
| JP7302491B2 (en) | 2020-01-24 | 2023-07-04 | 株式会社サタケ | Rice and soybean voluntary test device |
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| JP4537761B2 (en) * | 2004-05-13 | 2010-09-08 | 築野食品工業株式会社 | Tocotrienol-containing poultry eggs |
| JP2006000049A (en) * | 2004-06-17 | 2006-01-05 | Chubu Shiryo Kk | Mixed feed for beef cattle |
| JPWO2007132688A1 (en) * | 2006-05-15 | 2009-09-24 | 日本水産株式会社 | Fish meal with suppressed oxidation and method for producing the same |
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| JP7302491B2 (en) | 2020-01-24 | 2023-07-04 | 株式会社サタケ | Rice and soybean voluntary test device |
| KR102207915B1 (en) * | 2020-03-17 | 2021-01-26 | 다윈그룹(주) | Manufacturing method of cosmetic composition using rice and steam distiller using the same |
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