JP2015507930A5 - - Google Patents
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- Publication number
- JP2015507930A5 JP2015507930A5 JP2014557769A JP2014557769A JP2015507930A5 JP 2015507930 A5 JP2015507930 A5 JP 2015507930A5 JP 2014557769 A JP2014557769 A JP 2014557769A JP 2014557769 A JP2014557769 A JP 2014557769A JP 2015507930 A5 JP2015507930 A5 JP 2015507930A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- atn
- thio
- amino
- dna polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 11
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 6
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 claims 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 4
- NOLHIMIFXOBLFF-KVQBGUIXSA-N (2r,3s,5r)-5-(2,6-diaminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol Chemical compound C12=NC(N)=NC(N)=C2N=CN1[C@H]1C[C@H](O)[C@@H](CO)O1 NOLHIMIFXOBLFF-KVQBGUIXSA-N 0.000 claims 2
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims 2
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 2
- PISWNSOQFZRVJK-XLPZGREQSA-N 1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-2-sulfanylidenepyrimidin-4-one Chemical compound S=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 PISWNSOQFZRVJK-XLPZGREQSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000005549 deoxyribonucleoside Substances 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261599119P | 2012-02-15 | 2012-02-15 | |
| US61/599,119 | 2012-02-15 | ||
| US13/446,474 | 2012-04-13 | ||
| US13/446,474 US9353393B2 (en) | 2012-02-15 | 2012-04-13 | Methods and kits for reducing non-specific nucleic acid amplification |
| PCT/US2013/026136 WO2013123187A1 (en) | 2012-02-15 | 2013-02-14 | Methods and kits for reducing non-specific nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015507930A JP2015507930A (ja) | 2015-03-16 |
| JP2015507930A5 true JP2015507930A5 (OSRAM) | 2017-08-03 |
| JP6225123B2 JP6225123B2 (ja) | 2017-11-01 |
Family
ID=48945882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557769A Active JP6225123B2 (ja) | 2012-02-15 | 2013-02-14 | 非特異的核酸増幅を低減するための方法及びキット |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9353393B2 (OSRAM) |
| EP (1) | EP2814976B1 (OSRAM) |
| JP (1) | JP6225123B2 (OSRAM) |
| CN (1) | CN104093850B (OSRAM) |
| CA (1) | CA2864676C (OSRAM) |
| WO (1) | WO2013123187A1 (OSRAM) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2877593B1 (en) | 2012-07-26 | 2018-07-18 | Illumina, Inc. | Compositions and methods for the amplification of nucleic acids |
| US9938568B2 (en) | 2013-07-26 | 2018-04-10 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
| US9587263B2 (en) * | 2014-03-26 | 2017-03-07 | General Electric Company | Isothermal amplification under low salt condition |
| US10683534B2 (en) * | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| WO2016053638A1 (en) * | 2014-09-30 | 2016-04-07 | Ge Healthcare Bio-Sciences Corp. | Method for nucleic acid analysis directly from an unpurified biological sample |
| CN108473932B (zh) | 2015-09-09 | 2022-07-15 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
| US10077459B2 (en) * | 2016-05-04 | 2018-09-18 | General Electric Company | Cell-free protein expression using rolling circle amplification product |
| WO2018209092A1 (en) * | 2017-05-10 | 2018-11-15 | Board Of Regents, The University Of Texas System | Methods and devices related to amplifying nucleic acid at a variety of temperatures |
| US11001868B2 (en) * | 2017-08-11 | 2021-05-11 | Global Life Sciences Solutions Operations UK Ltd | Cell-free protein expression using double-stranded concatameric DNA |
| CN111334562A (zh) * | 2018-12-19 | 2020-06-26 | 合肥铼科生物科技有限公司 | 一种含有修饰引物的核酸扩增方法和试剂盒 |
| CN110295241A (zh) * | 2019-07-11 | 2019-10-01 | 深圳易致生物科技有限公司 | 用于检测尿路感染的引物组和包含该引物组的试剂盒 |
| FR3110602B1 (fr) | 2020-05-20 | 2025-03-07 | Anova Plus | Methode de detection sur le terrain de la presence et de la mutliresistance d’un bio-agresseur, notamment dezymo-septoria tritici,dans des culturescerealieres |
| CN112899346A (zh) * | 2021-01-05 | 2021-06-04 | 南京普济生物有限公司 | 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用 |
| FR3129407B1 (fr) | 2021-11-25 | 2024-12-13 | Anova Plus | Methode de detection de la presence d’un pathogene et determination de ses caracteristiques en sante humaine et animale |
| CN114277103A (zh) * | 2022-01-21 | 2022-04-05 | 杭州飞时达生物科技有限公司 | 一种基于六聚体随机引物高效滚环扩增方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040086880A1 (en) * | 1999-07-20 | 2004-05-06 | Sampson Jeffrey R | Method of producing nucleic acid molecules with reduced secondary structure |
| WO2002042490A1 (en) | 2000-11-24 | 2002-05-30 | Asper OÜ | Method of optimising the sequences of synthetic nucleic acids |
| US7993839B2 (en) * | 2001-01-19 | 2011-08-09 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| US20020197618A1 (en) * | 2001-01-20 | 2002-12-26 | Sampson Jeffrey R. | Synthesis and amplification of unstructured nucleic acids for rapid sequencing |
| US6617137B2 (en) | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US7176002B2 (en) | 2002-05-16 | 2007-02-13 | Applera Corporation | Universal-tagged oligonucleotide primers and methods of use |
| EP1426448A1 (en) | 2002-12-03 | 2004-06-09 | bioMérieux BV | Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay |
| US8871469B1 (en) | 2004-11-13 | 2014-10-28 | Steven Albert Benner | Self-avoiding molecular recognition systems in DNA priming |
| CN101213311B (zh) | 2005-04-29 | 2013-02-13 | 合成基因组股份有限公司 | 利用滚环扩增扩增和克隆单个dna分子 |
| DE602007013223D1 (de) | 2006-06-01 | 2011-04-28 | Trilink Biotechnologies San Diego | Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation |
| AU2008272421B2 (en) | 2007-07-03 | 2014-09-04 | Genaphora Ltd. | Chimeric primers for improved nucleic acid amplification reactions |
| WO2009046149A1 (en) | 2007-10-01 | 2009-04-09 | Applied Biosystems Inc. | Chase ligation sequencing |
| AU2010307991A1 (en) | 2009-10-13 | 2012-05-10 | Syntezza Molecular Detection Israel Ltd. | Methods and compositions for amplifying target sequences from nucleic acid samples |
| US20110195457A1 (en) | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
-
2012
- 2012-04-13 US US13/446,474 patent/US9353393B2/en active Active
-
2013
- 2013-02-14 JP JP2014557769A patent/JP6225123B2/ja active Active
- 2013-02-14 CA CA2864676A patent/CA2864676C/en active Active
- 2013-02-14 CN CN201380009386.6A patent/CN104093850B/zh active Active
- 2013-02-14 WO PCT/US2013/026136 patent/WO2013123187A1/en not_active Ceased
- 2013-02-14 EP EP13749671.7A patent/EP2814976B1/en active Active
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