JP2015172488A - automatic analyzer and abnormality detection method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To solve the problem in which an abnormality of a diluted specimen used for analysis cannot be detected.SOLUTION: In the automatic analyzer, a sample dilution pipette 7 sucks an analyte from a specimen vessel 21, and dispenses it to a dilution vessel 23 for storing diluted solution to prepare a diluted specimen. A sampling pipette 8 sucks the diluted specimen from the dilution vessel 23, and dispenses the diluted specimen to a plurality of reaction vessels 26 for storing a reagent. A multiwavelength photometer 16 measures the absorbance of the diluted specimens dispensed to the plurality of reaction vessels 26, and a detection unit 32 compares the measurement results with each other and detects an abnormality of the diluted specimens.

Description

  The present invention relates to an automatic analyzer and an abnormality detection method for analyzing a component of a specimen by reacting the specimen with a reagent.

  As an automatic analyzer, a biochemical analyzer that analyzes various components contained in a specimen such as blood and urine is known. In this biochemical analyzer, samples such as serum and urine are diluted under certain conditions, then dispensed into a reaction vessel, and a reagent according to the analysis item and the sample are mixed in the reaction vessel (cuvette). To react. The biochemical analyzer analyzes the measurement target substance contained in the sample by measuring the absorbance of the diluted sample dispensed in the reaction container and converting the absorbance into a concentration. This absorbance is proportional to the concentration of the sample contained in the diluted sample. For this reason, correct analysis cannot be performed unless a certain dispensing accuracy is maintained so as to keep the concentration of the specimen constant.

  Here, the suction of the specimen is performed by the specimen dilution pipette sucking the specimen from the liquid level of the specimen detected by the liquid level sensor. However, if bubbles exist on the liquid level of the specimen, the liquid level sensor misidentifies the bubble surface as the liquid level, and the sample dilution pipette sucks the bubbles instead of the specimen. In this case, the amount of the sample actually aspirated is reduced from the predetermined amount that the sample dilution pipette originally needs to aspirate, and all the measured values of the analysis target items are lowered by the amount of the amount of the sample decreased. Further, when a solid substance such as fibrin is suspended in the specimen, the amount of the specimen to be aspirated is also reduced by clogging the solid substance at the tip of the sample dilution pipette.

  A clot sensor is used to cope with such a decrease in the amount of sample to be aspirated. The clot sensor has a function of monitoring whether the normal specimen is aspirated by monitoring the atmospheric pressure in the sample dilution pipette. When this clot sensor is used, it can be determined that clogging has occurred in the sample dilution pipette when fibrin, blood clot, etc. adhere to the tip of the probe and the internal pressure during suction is lower than normal.

  Here, Patent Document 1 discloses a technique for monitoring that a specimen is discharged into a measurement cell.

JP 2012-37478 A

  By the way, when the sample dilution pipette sucks air bubbles instead of a solid substance, it is considered that the internal pressure of the sample dilution pipette rises because the suction resistance is lower than that of the specimen. However, since the difference in internal pressure at the tip of the sample dilution pipette at the time of sample suction and bubble suction is small, it is difficult to detect the suction of bubbles using only the clot sensor.

  In order to detect bubbles sucked by the sample dilution pipette, for example, it was considered to incorporate a bubble sensor using a microwave. However, when the bubble sensor is incorporated around the sample dilution pipette, the overall size including the sample dilution pipette and the bubble sensor is increased. Moreover, even if the bubble sensor is downsized, the manufacturing cost of the automatic analyzer increases.

  The present invention has been made in view of such a situation, and an object thereof is to enable detection of an abnormality of a diluted sample used for analysis.

  In the automatic analyzer according to the present invention, the first dispensing unit sucks the sample from the sample container, dispenses the diluted sample into the dilution container containing the diluent, and creates the diluted sample, and the second dispensing unit performs the dilution. The diluted sample is aspirated from the container, and the diluted sample is dispensed into a plurality of reaction containers in which reagents are stored. And a detection part compares the measurement result which measured the light absorbency of the diluted sample dispensed to the some reaction container with the photometer, and detects abnormality of a diluted sample.

  According to the present invention, an abnormality of a diluted specimen can be detected by measuring the absorbance of a plurality of reaction containers into which the diluted specimen has been dispensed.

It is explanatory drawing which shows typically the example of 1 embodiment of the automatic analyzer of this invention. It is a block diagram which shows the internal structural example of the computer which concerns on the example of 1 embodiment of this invention. It is explanatory drawing which shows the procedure which transfers the diluted sample produced | generated from the sample which concerns on one embodiment of the automatic analyzer of this invention to a reaction container. It is explanatory drawing which shows the example of the diluted sample to which the reagent which concerns on one embodiment of the automatic analyzer of this invention was added. It is a graph which shows the light absorbency of the diluted specimen which concerns on one embodiment of the automatic analyzer of this invention. It is a flowchart which shows the example of the abnormality detection process of the diluted sample which the detection part which concerns on one embodiment of the automatic analyzer of this invention performs.

  Hereinafter, an automatic analyzer according to an embodiment of the present invention will be described with reference to the accompanying drawings. In the present specification and drawings, components having substantially the same function or configuration are denoted by the same reference numerals, and redundant description is omitted.

[1. Example of embodiment]
<1-1. Configuration of automatic analyzer>
First, the automatic analyzer of this example will be described with reference to FIG.
FIG. 1 is an explanatory view schematically showing the automatic analyzer of this example.
The apparatus shown in FIG. 1 is a biochemical analyzer 1 applied as an example of the automatic analyzer of the present invention. The biochemical analyzer 1 is an apparatus that automatically measures the amount of a specific component contained in a biological sample such as blood or urine.

  As shown in FIG. 1, the biochemical analyzer 1 includes a sample turntable 2, a dilution turntable 3, a first reagent turntable 4, a second reagent turntable 5, and a reaction turntable 6. ing. The biochemical analyzer 1 also includes a sample dilution pipette 7, a sampling pipette 8, a dilution stirring device 9, a dilution washing device 11, a first reagent pipette 12, a second reagent pipette 13, and a first reaction stirring. The apparatus 14, the second reaction stirrer 15, the multiwavelength photometer 16, the thermostatic bath 17, the reaction vessel cleaning device 18, and the calculator 30 are provided.

  The sample turntable 2 (an example of a specimen container holding part) is formed in a substantially cylindrical container shape with one axial end opened. The sample turntable 2 contains a plurality of specimen containers 21 and a plurality of diluent containers 22. The specimen container 21 contains a specimen (sample) made of blood, urine, or the like. The diluent container 22 stores a special diluent other than physiological saline which is a normal diluent.

  The plurality of sample containers 21 are arranged side by side with a predetermined interval in the circumferential direction of the sample turntable 2. Further, two rows of the specimen containers 21 arranged in the circumferential direction of the sample turntable 2 are set at a predetermined interval in the radial direction of the sample turntable 2.

  The plurality of diluent containers 22 are arranged on the inner side in the radial direction of the sample turntable 2 than the row of the plurality of sample containers 21. The plurality of diluent containers 22 are arranged side by side at a predetermined interval in the circumferential direction of the sample turntable 2, similarly to the plurality of sample containers 21. The rows of the diluent containers 22 arranged in the circumferential direction of the sample turntable 2 are set in two rows at predetermined intervals in the radial direction of the sample turntable 2.

  Note that the arrangement of the plurality of specimen containers 21 and the plurality of diluent containers 22 is not limited to two rows, but may be one row or may be arranged in three or more rows in the radial direction of the sample turntable 2. .

  The sample turntable 2 is supported so as to be rotatable along the circumferential direction by a drive mechanism (not shown). The sample turntable 2 is rotated at a predetermined speed in a predetermined angular range in the circumferential direction by a driving mechanism (not shown). A dilution turntable 3 (an example of a dilution container holding unit) is disposed around the sample turntable 2.

  The dilution turntable 3, the first reagent turntable 4, the second reagent turntable 5, and the reaction turntable 6 (an example of a reaction container holding unit) are substantially cylindrical with one end opened in the axial direction, like the sample turntable 2. It is formed in a container shape. The dilution turntable 3 and the reaction turntable 6 are rotated at a predetermined speed by a predetermined angular range in the circumferential direction by a drive mechanism (not shown). The reaction turntable 6 is set so as to rotate more than a half turn by one movement.

  A plurality of dilution containers 23 are accommodated in the dilution turntable 3 side by side in the circumferential direction of the dilution turntable 3. The dilution container 23 accommodates a diluted specimen (hereinafter referred to as “diluted specimen”) that is aspirated from the specimen container 21 disposed on the sample turntable 2.

  In the first reagent turntable 4, a plurality of first reagent containers 24 are accommodated in the circumferential direction of the first reagent turntable 4. A plurality of second reagent containers 25 are accommodated in the second reagent turntable 5 side by side in the circumferential direction of the second reagent turntable 5. The first reagent container 24 stores the concentrated first reagent, and the second reagent container 25 stores the concentrated second reagent.

  Furthermore, the first reagent turntable 4, the first reagent container 24, the second reagent turntable 5, and the second reagent container 25 are kept at a predetermined temperature by a cold insulation mechanism (not shown). Therefore, the first reagent stored in the first reagent container 24 and the second reagent stored in the second reagent container 25 are kept cold at a predetermined temperature.

  The reaction turntable 6 is arranged between the dilution turntable 3, the first reagent turntable 4 and the second reagent turntable 5. A plurality of reaction vessels 26 are accommodated in the reaction turntable 6 side by side in the circumferential direction of the reaction turntable 6. The reaction container 26 includes a diluted sample sampled from the dilution container 23 of the dilution turntable 3, a first reagent sampled from the first reagent container 24 of the first reagent turntable 4, and a second reagent of the second reagent turntable 5. The sampled second reagent is injected from the reagent container 25. In the reaction container 26, the diluted specimen, the first reagent, and the second reagent are agitated to perform the reaction.

  A sample dilution pipette 7 (an example of a first dispensing unit) is disposed around the sample turntable 2 and the dilution turntable 3. The sample dilution pipette 7 is supported by an unillustrated dilution pipette drive mechanism so as to be movable in the axial direction (for example, up and down direction) of the sample turntable 2 and the dilution turntable 3. The sample dilution pipette 7 is supported by a dilution pipette driving mechanism so as to be rotatable along a horizontal direction substantially parallel to the openings of the sample turntable 2 and the dilution turntable 3. The sample dilution pipette 7 reciprocates between the sample turntable 2 and the dilution turntable 3 by rotating along the horizontal direction. When the sample dilution pipette 7 moves between the sample turntable 2 and the dilution turntable 3, the sample dilution pipette 7 passes through a cleaning device (not shown).

Here, the operation of the sample dilution pipette 7 will be described.
When the sample dilution pipette 7 moves to a predetermined position above the opening in the sample turntable 2, the sample dilution pipette 7 descends along the axial direction of the sample turntable 2, and the pipette provided at the tip of the sample dilution pipette 7 is moved to the specimen container 21. Insert inside. At this time, the sample dilution pipette 7 operates a sample pump (not shown) to suck a predetermined amount of the sample stored in the sample container 21. Next, the sample dilution pipette 7 rises along the axial direction of the sample turntable 2 and extracts the pipette from the specimen container 21. Then, the sample dilution pipette 7 rotates along the horizontal direction and moves to a predetermined position above the opening in the dilution turntable 3.

  Next, the sample dilution pipette 7 descends along the axial direction of the dilution turntable 3 and inserts the pipette into a predetermined dilution container 23. Then, the sample dilution pipette 7 discharges the aspirated specimen and a predetermined amount of diluent (for example, physiological saline) supplied from the sample dilution pipette 7 itself into the dilution container 23. As a result, the specimen is diluted to a predetermined multiple concentration in the dilution container 23. Thereafter, the sample dilution pipette 7 is washed by a washing device.

  A sampling pipette 8 (an example of a second dispensing unit) is disposed between the dilution turntable 3 and the reaction turntable 6. The sampling pipette 8 is supported by a sampling pipette drive mechanism (not shown) so as to be movable and rotatable in the axial direction (vertical direction) and the horizontal direction of the dilution turntable 3, similarly to the sample dilution pipette 7. The sampling pipette 8 reciprocates between the dilution turntable 3 and the reaction turntable 6.

  The sampling pipette 8 inserts a pipette into the dilution container 23 of the dilution turntable 3 and sucks a predetermined amount of diluted specimen. Then, the sampling pipette 8 discharges the sucked diluted specimen into the reaction container 26 of the reaction turntable 6.

  The first reagent pipette 12 is disposed between the reaction turntable 6 and the first reagent turntable 4, and the second reagent pipette 13 is disposed between the reaction turntable 6 and the second reagent turntable 5. The first reagent pipette 12 is supported by an unillustrated first reagent pipette drive mechanism so as to be movable and rotatable in the axial direction (vertical direction) and horizontal direction of the reaction turntable 6. The first reagent pipette 12 reciprocates between the first reagent turntable 4 and the reaction turntable 6.

  The first reagent pipette 12 inserts a pipette into the first reagent container 24 of the first reagent turntable 4 and aspirates a predetermined amount of the first reagent. Then, the first reagent pipette 12 discharges the sucked first reagent into the reaction container 26 of the reaction turntable 6.

  Further, the second reagent pipette 13 can be moved and rotated in the axial direction (vertical direction) and the horizontal direction of the reaction turntable 6 by a second reagent pipette drive mechanism (not shown), similarly to the first reagent pipette 12. It is supported. The second reagent pipette 13 reciprocates between the second reagent turntable 5 and the reaction turntable 6.

  The second reagent pipette 13 inserts a pipette into the second reagent container 25 of the second reagent turntable 5 and sucks a predetermined amount of the second reagent. Then, the second reagent pipette 13 discharges the sucked second reagent into the reaction container 26 of the reaction turntable 6.

  The dilution stirring device 9 and the dilution cleaning device 11 are arranged around the dilution turntable 3. The dilution stirrer 9 inserts a stirring bar (not shown) into the dilution container 23 and stirs the specimen and the diluted solution.

  The dilution cleaning device 11 is a device for cleaning the dilution container 23 after the diluted specimen is aspirated by the sampling pipette 8. The dilution cleaning device 11 has a plurality of dilution container cleaning nozzles. The plurality of dilution container cleaning nozzles are connected to a waste liquid pump (not shown) and a detergent pump (not shown). The dilution cleaning device 11 inserts a dilution container cleaning nozzle into the dilution container 23 and drives the waste liquid pump to suck in the diluted specimen remaining in the dilution container 23 by the inserted dilution container cleaning nozzle. Then, the dilution cleaning device 11 discharges the sucked diluted specimen to a waste liquid tank (not shown).

  Thereafter, the dilution cleaning device 11 supplies the detergent from the detergent pump to the dilution container cleaning nozzle, and discharges the detergent into the dilution container 23 from the dilution container cleaning nozzle. The inside of the dilution container 23 is washed with this detergent. Thereafter, the dilution cleaning device 11 sucks the detergent through the dilution container cleaning nozzle and dries the inside of the dilution container 23.

  The first reaction stirring device 14, the second reaction stirring device 15, and the reaction vessel cleaning device 18 are arranged around the reaction turntable 6. The first reaction stirrer 14 inserts a stirring bar (not shown) into the reaction vessel 26 and stirs the diluted specimen and the first reagent. As a result, the reaction between the diluted specimen and the first reagent is performed uniformly and rapidly. In addition, since the structure of the 1st reaction stirring apparatus 14 is the same as the dilution stirring apparatus 9, the description is abbreviate | omitted here.

  The second reaction stirrer 15 inserts a stirring bar (not shown) into the reaction vessel 26 and stirs the diluted specimen, the first reagent, and the second reagent. As a result, the reaction between the diluted specimen, the first reagent, and the second reagent is performed uniformly and rapidly. In addition, since the structure of the 2nd reaction stirring apparatus 15 is the same as the dilution stirring apparatus 9, the description is abbreviate | omitted here.

  The reaction vessel cleaning device 18 is a device for cleaning the inside of the reaction vessel 26 that has been inspected. The reaction container cleaning device 18 has a plurality of reaction container cleaning nozzles. The plurality of reaction container cleaning nozzles are connected to a waste liquid pump (not shown) and a detergent pump (not shown), similarly to the dilution container cleaning nozzle. In addition, since the washing | cleaning process in the reaction container washing | cleaning apparatus 18 is the same as that of the dilution washing | cleaning apparatus 11 mentioned above, the description is abbreviate | omitted.

  The multiwavelength photometer 16 is disposed so as to face the outer wall of the reaction turntable 6 around the reaction turntable 6. The multi-wavelength photometer 16 is injected into the reaction container 26 and optically measures the diluted specimen that has reacted with the first chemical solution and the second chemical solution, and the amounts of various components in the specimen are referred to as “absorbance”. It is output as numerical data to detect the reaction state of the diluted specimen. A computer 30 is connected to the multiwavelength photometer 16.

  Furthermore, a constant temperature bath 17 is disposed around the reaction turntable 6. The thermostatic chamber 17 is configured to always keep the temperature of the reaction vessel 26 provided on the reaction turntable 6 constant.

<1-2. Computer configuration example>
Next, a configuration example of the computer 30 will be described.
FIG. 2 is a block diagram illustrating an internal configuration example of the computer 30.
The computer 30 includes a control unit 31, a recording unit 34, a display unit 35, an input unit 36, and an interface unit 37 connected to the bus 38.

  The control unit 31 is configured by a CPU (Central Processing Unit) or the like, and controls the operation of each unit in the biochemical analyzer 1. The control unit 31 includes a detection unit 32 and an alarm unit 33.

The detector 32 detects an abnormality of the diluted specimen dispensed into the reaction container 26.
The alarm unit 33 outputs an alarm when the detection unit 32 detects an abnormality in dilution, and instructs the user to recreate a diluted sample.

The recording unit 34 is configured by a large-capacity recording device such as an HDD (Hard disk drive), for example, and the control unit 31 program, parameters, calibration curve, detection result of dilution abnormality, and input made by the input unit 36. Record operations etc.
The display unit 35 displays the detection result of dilution abnormality and the alarm output from the alarm unit 33. For the display unit 35, for example, a liquid crystal display device or the like is used.
The input unit 36 receives an operation input to the biochemical analyzer 1 performed by a user and outputs an input signal to the control unit 31. For the input unit 36, for example, a mouse, a keyboard, a touch panel, or the like is used.

  The interface unit 37 is used to pass the measurement result to the detection unit 32 when the measurement result of the diluted specimen measured by the multiwavelength photometer 16 is input. FIG. 2 shows an example in which only the multi-wavelength photometer 16 is connected to the interface unit 37, but each unit in the biochemical analyzer 1 is also connected to the interface unit 37 in the same manner, and control by the computer 30 is performed. Done.

<1-3. Generation of diluted specimen>
Next, a procedure for generating a diluted specimen will be described with reference to FIG.
FIG. 3 shows a procedure for transferring the diluted specimen generated from the specimen to the reaction vessel 26. In FIG. 3, the moving direction of the specimen or diluted specimen is indicated by a thin arrow, and the order of transfer to the container is indicated by a thick thick arrow.

  First, the sample dilution pipette 7 aspirates the specimen from the specimen container 21 accommodated in the sample turntable 2 (S1). At this time, the volume of the sample to be aspirated is, for example, 30 μL. Then, the sample dilution pipette 7 transfers the aspirated specimen to the dilution turntable 3.

  The sample dilution pipette 7 discharges the specimen transferred to the dilution turntable 3 to the dilution container 23. Further, the sample dilution pipette 7 puts a nonionic surfactant (for example, polyoxyethylene alkylphenyl ether 0.04%) as a reaction reagent supplied from a supply unit (not shown) into the dilution container 23 from which the specimen has been discharged. The containing physiological saline is discharged (S2). When the surfactant is added to the diluted specimen in this manner, the diluted specimen is less likely to stick to the inner wall of the reaction vessel 26, and an accurate liquid amount can be measured. Since the volume of the physiological saline is 120 μL, the diluted container 23 accommodates a diluted specimen having a volume of 150 μL, which is a combination of the specimen and the physiological saline.

  The diluted specimen discharged into the dilution container 23 is stirred by a stirrer (not shown) inserted by the dilution stirring device 9. Thereafter, the sampling pipette 8 sucks the diluted sample from the dilution container 23 within the range of 2 to 25 μL, and transfers the diluted sample to the reaction turntable 6 (S3).

  The sampling pipette 8 dispenses an amount of diluted specimen necessary for analysis of each test item into six reaction containers 26 installed on the reaction turntable 6 (S4). Of these reaction vessels 26, optical measurement using the multiwavelength photometer 16 is performed on the diluted specimen dispensed into the two reaction vessels 26.

<1-4. Explanation of diluted specimen>
FIG. 4 shows an example of the sample accommodated in the sample container 21, the dilution container 23, and the reaction container 26 corresponding to each step of FIG.
In step S <b> 1 shown in FIG. 3, for example, three types of samples are accommodated in each sample container 21. In the drawing, the sample container 21 is labeled as “sample 1” to “sample 3” to distinguish the sample container 21. In step S <b> 2, a diluted sample in which the same diluent is used is stored in the dilution container 23 for each sample container 21. Note that at least two dilution containers 23 are provided for each sample, and diluted samples obtained by diluting one sample are accommodated in the two dilution containers 23a and 23b.

  After step S3, in step S4, the diluted specimen transferred from the dilution container 23a is dispensed into the reaction container 26a, and the diluted specimen transferred from the dilution container 23b is dispensed into the reaction container 26b. The reaction containers 26a and 26b are labeled with detection items “D1” and “D2” indicating the same dilution rate. Here, the diluted specimen stored in the dilution container 23a is dispensed into the reaction container 26a as it is. However, a part of the diluted specimen stored in the dilution container 23b is dispensed into the reaction container 26b, and the remaining diluted specimen is dispensed into the reaction container 26c other than the reaction containers 26a and 26b. The reaction container 26c is labeled with various test items related to enzymes and lipids, for example. Then, after the reaction containers 26a and 26b are agitated, the absorbance of the diluted specimen accommodated in the reaction containers 26a and 26b is measured.

<1-5. Explanation of absorbance of diluted specimen>
FIG. 5 is a graph showing the absorbance of the diluted specimen. In this graph, the horizontal axis is a dilution series (unit: 1/10), and the vertical axis is absorbance.

  The diluted specimen has an absorbance derived from the color tone specific to the component contained in the specimen, and this absorbance has dilution linearity. The diluted sample exhibits a constant absorbance before the reagent is added, and is proportional to the amount of the sample contained in the diluted sample. The graph of FIG. 5 shows that the absorbance of the diluted specimen is proportional to the ratio of the specimen contained in the diluted specimen. In other words, the absorbance of the diluted specimen decreases as the concentration decreases as the specimen is diluted with physiological saline. Therefore, when there is some problem in the biochemical analyzer 1 or the diluted specimen, and the specimen content is reduced from a predetermined amount, the absorbance is also considered to be reduced in proportion to the specimen content.

  Here, for each sample, when two diluted samples are continuously prepared from the same sample under the same conditions (for example, the same temperature and the same time), the absorbance of each of the two diluted samples is equal in a normal state. However, if a problem occurs in the suction or dilution of the specimen using the sample dilution pipette 7 and the diluted specimen stored in the reaction containers 26a and 26b is not normally created, the detection items “D1” and “D2” A difference is generated in the absorbance of the diluted specimen labeled "". Therefore, the biochemical analyzer 1 can determine the success or failure of the dilution and the success or failure of the sample aspiration by detecting the difference in absorbance between the diluted samples labeled with the detection items “D1” and “D2”. .

In measuring the absorbance of the diluted specimen, the above-described physiological saline is added to the diluted specimen and measurement is performed in a state suitable for measurement. Then, the dilution abnormality and the suction abnormality can be detected by the following expression (1) given as an example.
(D1 / D2) × 100 = detection value (%) (1)

  Here, the detection unit 32 measures the absorbance using, for example, 10 sets of diluted specimens in which the detection items “D1” and “D2” are labeled from the same specimen. There are 100 combinations of the detection items “D1” and “D2” at the time of measurement, and the normal range (99.73%, ± 3 SD (standard deviation: Standard Deviation) obtained from the statistical value of the detection value (N = 100)) )) Can be set to 105 to 96%, for example. When the detection unit 32 determines that the detected value exceeds the normal range, the alarm unit 33 outputs an alarm. Thereby, it is possible to quickly detect an abnormality in dilution or an abnormality in suction. When the detection unit 32 determines that the diluted sample is abnormal, the alarm unit 33 instructs the re-creation of the diluted sample. Thereafter, by newly measuring the absorbance of the newly prepared diluted specimen, it is possible to prevent erroneous reporting due to an abnormally diluted specimen.

<1-6. Explanation of abnormality detection processing>
FIG. 6 shows an example of diluted specimen abnormality detection processing performed by the detection unit 32.
First, the detection unit 32 acquires the absorbance data of the detection item “D1” labeled on the reaction vessel 26a from the recording unit 34 (S11). Next, the detection part 32 acquires the data measured from all the other test items together with the absorbance data of the detection item “D2” labeled on the reaction vessel 26b (S12).

  Then, the detection unit 32 compares the absorbances of the detection items “D1” and “D2” (S13). If the absorbances of the detection items “D1” and “D2” are substantially equal, measurement data for all the diluted samples is output (S14). If the absorbances of the detection items “D1” and “D2” are different, it can be estimated that an abnormality has occurred in the diluted specimen. Therefore, the detection unit 32 outputs all measurement data with a retest flag in order to instruct retesting of the sample including the recreation of the diluted sample (S15). The alarm unit 33 outputs an alarm when the measurement data to which the reexamination flag is attached by the detection unit 32 is output.

  According to the biochemical analyzer 1 according to the embodiment described above, the absorbance of the diluted specimen dispensed into the reaction vessels 26a and 26b is measured, and the result is compared to determine the abnormality of the diluted specimen. Can be detected. And since the reexamination flag is attached about the diluted sample which detected abnormality, reexamination can be implemented and a correct measured value can be obtained.

[2. Modified example]
In the above-described embodiment, an example in which two diluted specimens created from the same specimen under the same conditions are stored in the dilution container 23 installed on the dilution turntable 3 is shown. However, the dilution turntable 3 is essential. is not. For example, even a biochemical analyzer having a line table that holds the dilution container 23 in a straight line can compare the detection items “D1” and “D2” and detect an abnormality of the diluted sample.

  In addition, the detection of dilution abnormality performed by comparing the absorbances of two diluted specimens may be caused by bubbles or solids. Furthermore, not only the cause on the specimen side but also the cause on the apparatus side (abnormality of the suction pump, abnormality of the flow system, etc.) can be detected.

  In addition, in devices that analyze serum (eg, biochemical automatic analyzers, immune automatic analyzers, etc.), when the amount of serum used for analysis and the measured values (concentration, enzyme activity, etc.) of the measurement target item are in a proportional relationship Can be used. Here, the proportional relationship only needs to have a correlation based on a certain law, and includes a curve and the like.

  Further, not only two diluted samples but also three or more diluted samples may be measured and compared to detect abnormality of the diluted sample.

Further, the present invention is not limited to the above-described embodiments, and various other application examples and modifications can be taken without departing from the gist of the present invention described in the claims.
For example, the above-described embodiments are detailed and specific descriptions of the configuration of the apparatus and the system in order to explain the present invention in an easy-to-understand manner, and are not necessarily limited to those having all the configurations described. Absent. Further, a part of the configuration of one embodiment can be replaced with the configuration of another embodiment, and the configuration of another embodiment can be added to the configuration of one embodiment. Moreover, it is also possible to add, delete, and replace other configurations for a part of the configuration of each embodiment.
Further, the control lines and information lines indicate what is considered necessary for the explanation, and not all the control lines and information lines on the product are necessarily shown. Actually, it may be considered that almost all the components are connected to each other.

  DESCRIPTION OF SYMBOLS 1 ... Biochemical analyzer, 2 ... Sample turntable, 3 ... Dilution turntable, 6 ... Reaction turntable, 7 ... Sample dilution pipette, 8 ... Sampling pipette, 16 ... Multiwavelength photometer, 21 ... Sample container, 23 ... Dilution container, 26 ... reaction container, 30 ... computer

Claims (6)

A first dispensing unit that aspirates the specimen from a specimen container that contains the specimen and dispenses the specimen into a dilution container that contains a diluent;
A second dispensing unit that aspirates the diluted specimen from the dilution container and dispenses the diluted specimen into a plurality of reaction containers;
A photometer for measuring the absorbance of the diluted specimen dispensed in a plurality of reaction containers and outputting the measurement results;
An automatic analyzer comprising: a detection unit that compares measurement results of the plurality of reaction containers and detects an abnormality of the diluted specimen. The automatic analyzer according to claim 1, further comprising an alarm unit that outputs an alarm when the detection unit detects an abnormality of the diluted sample. The automatic analyzer according to claim 2, wherein the alarm unit instructs re-creation of the diluted sample when the detection unit detects an abnormality of the diluted sample. The automatic analyzer according to claim 3, wherein the dilution container is held on a turntable that holds the dilution container on a circumference or a line table that holds the dilution container on a straight line. The automatic analyzer according to claim 4, wherein the diluent is a physiological saline containing a surfactant. A first dispensing unit aspirates the sample from the sample container and dispenses the diluted sample into a dilution container containing a diluent;
A second dispensing unit aspirates the diluted specimen from the dilution container and dispenses the diluted specimen into a plurality of reaction containers containing reagents;
And a step of detecting an abnormality of the diluted sample by comparing a measurement result obtained by measuring the absorbance of the diluted sample dispensed into a plurality of reaction containers by a photometer.

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