JP2014528964A - バチルス種のサーファクタント、それを含む組成物、それを得るための方法およびその使用 - Google Patents
バチルス種のサーファクタント、それを含む組成物、それを得るための方法およびその使用 Download PDFInfo
- Publication number
- JP2014528964A JP2014528964A JP2014533967A JP2014533967A JP2014528964A JP 2014528964 A JP2014528964 A JP 2014528964A JP 2014533967 A JP2014533967 A JP 2014533967A JP 2014533967 A JP2014533967 A JP 2014533967A JP 2014528964 A JP2014528964 A JP 2014528964A
- Authority
- JP
- Japan
- Prior art keywords
- mycosubtilin
- bacillus subtilis
- medium
- membrane
- air
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 77
- 239000000203 mixture Substances 0.000 title claims abstract description 60
- 241000193830 Bacillus <bacterium> Species 0.000 title description 9
- 239000004094 surface-active agent Substances 0.000 title description 4
- 239000003876 biosurfactant Substances 0.000 claims abstract description 101
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 82
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 79
- 239000012528 membrane Substances 0.000 claims description 157
- 108700030603 mycosubtiline Proteins 0.000 claims description 132
- RCIPRGNHNAEGHR-ZLHAWHIKSA-N 3-[(3s,6s,13s,16r,19r,22r,25r,28s)-6,13,19,22-tetrakis(2-amino-2-oxoethyl)-16-(hydroxymethyl)-25-[(4-hydroxyphenyl)methyl]-10-(11-methyltridecyl)-2,5,8,12,15,18,21,24,27-nonaoxo-1,4,7,11,14,17,20,23,26-nonazabicyclo[26.3.0]hentriacontan-3-yl]propanamide Chemical compound C([C@H]1NC(=O)[C@@H]2CCCN2C(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)CC(NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](CO)NC(=O)[C@@H](CC(N)=O)NC(=O)[C@@H](CC(N)=O)NC1=O)CCCCCCCCCCC(C)CC)C1=CC=C(O)C=C1 RCIPRGNHNAEGHR-ZLHAWHIKSA-N 0.000 claims description 130
- 239000007788 liquid Substances 0.000 claims description 87
- 244000005700 microbiome Species 0.000 claims description 50
- 238000000108 ultra-filtration Methods 0.000 claims description 46
- 238000001471 micro-filtration Methods 0.000 claims description 28
- AFWTZXXDGQBIKW-UHFFFAOYSA-N C14 surfactin Natural products CCCCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 AFWTZXXDGQBIKW-UHFFFAOYSA-N 0.000 claims description 19
- 239000012510 hollow fiber Substances 0.000 claims description 19
- NJGWOFRZMQRKHT-UHFFFAOYSA-N surfactin Natural products CC(C)CCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-UHFFFAOYSA-N 0.000 claims description 19
- NJGWOFRZMQRKHT-WGVNQGGSSA-N surfactin C Chemical compound CC(C)CCCCCCCCC[C@@H]1CC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-WGVNQGGSSA-N 0.000 claims description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 239000000758 substrate Substances 0.000 claims description 14
- 239000011148 porous material Substances 0.000 claims description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 230000000903 blocking effect Effects 0.000 claims description 9
- 229940121375 antifungal agent Drugs 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 239000003429 antifungal agent Substances 0.000 claims description 7
- 230000000813 microbial effect Effects 0.000 claims description 7
- 101100257702 Bacillus subtilis (strain 168) srfAA gene Proteins 0.000 claims description 6
- 101100257706 Dictyostelium discoideum srfA gene Proteins 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 5
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 4
- 235000001014 amino acid Nutrition 0.000 claims description 4
- 229940024606 amino acid Drugs 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 125000004432 carbon atom Chemical group C* 0.000 claims description 4
- 150000004665 fatty acids Chemical group 0.000 claims description 4
- 230000008520 organization Effects 0.000 claims description 4
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 229960001230 asparagine Drugs 0.000 claims description 3
- 235000009582 asparagine Nutrition 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 2
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 claims description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 2
- 235000001727 glucose Nutrition 0.000 claims description 2
- 235000011187 glycerol Nutrition 0.000 claims description 2
- 150000007524 organic acids Chemical class 0.000 claims description 2
- 235000005985 organic acids Nutrition 0.000 claims description 2
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 claims 2
- 235000013681 dietary sucrose Nutrition 0.000 claims 1
- 229960004793 sucrose Drugs 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 38
- 239000002537 cosmetic Substances 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 3
- 239000003208 petroleum Substances 0.000 abstract description 3
- 230000000853 biopesticidal effect Effects 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 78
- 239000002609 medium Substances 0.000 description 77
- 239000000243 solution Substances 0.000 description 44
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 39
- 108010028921 Lipopeptides Proteins 0.000 description 30
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 30
- 239000001301 oxygen Substances 0.000 description 30
- 229910052760 oxygen Inorganic materials 0.000 description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 30
- 238000004458 analytical method Methods 0.000 description 23
- 210000004027 cell Anatomy 0.000 description 20
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 19
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 18
- 239000012634 fragment Substances 0.000 description 14
- 239000013612 plasmid Substances 0.000 description 14
- 239000002028 Biomass Substances 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 238000001914 filtration Methods 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 11
- 230000002572 peristaltic effect Effects 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 10
- 229920002274 Nalgene Polymers 0.000 description 10
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 10
- 239000007789 gas Substances 0.000 description 10
- 238000004128 high performance liquid chromatography Methods 0.000 description 10
- 238000000746 purification Methods 0.000 description 10
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
- 230000000843 anti-fungal effect Effects 0.000 description 9
- 229910000029 sodium carbonate Inorganic materials 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 239000002585 base Substances 0.000 description 8
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 7
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 7
- 239000007993 MOPS buffer Substances 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 229940041514 candida albicans extract Drugs 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 7
- 238000001228 spectrum Methods 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 6
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 6
- 239000004695 Polyether sulfone Substances 0.000 description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 238000011026 diafiltration Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- -1 fatty acid esters Chemical class 0.000 description 6
- 239000006260 foam Substances 0.000 description 6
- 235000013922 glutamic acid Nutrition 0.000 description 6
- 239000004220 glutamic acid Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000012071 phase Substances 0.000 description 6
- 229920006393 polyether sulfone Polymers 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000004627 regenerated cellulose Substances 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 238000005303 weighing Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 5
- 108010029485 Protein Isoforms Proteins 0.000 description 5
- 102000001708 Protein Isoforms Human genes 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 5
- 239000012141 concentrate Substances 0.000 description 5
- 239000012228 culture supernatant Substances 0.000 description 5
- 238000001704 evaporation Methods 0.000 description 5
- 230000008020 evaporation Effects 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 229920001903 high density polyethylene Polymers 0.000 description 5
- 239000004700 high-density polyethylene Substances 0.000 description 5
- 230000006801 homologous recombination Effects 0.000 description 5
- 238000002744 homologous recombination Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 5
- 235000019341 magnesium sulphate Nutrition 0.000 description 5
- 239000000693 micelle Substances 0.000 description 5
- 108091008146 restriction endonucleases Proteins 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 229930193140 Neomycin Natural products 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 230000003698 anagen phase Effects 0.000 description 4
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 108010002015 fengycin Proteins 0.000 description 4
- CUOJDWBMJMRDHN-VIHUIGFUSA-N fengycin Chemical compound C([C@@H]1C(=O)N[C@H](C(=O)OC2=CC=C(C=C2)C[C@@H](C(N[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C)C(=O)N2CCC[C@H]2C(=O)N[C@@H](CCC(N)=O)C(=O)N1)[C@@H](C)O)=O)NC(=O)[C@@H](CCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)C[C@H](O)CCCCCCCCCCCCC)[C@@H](C)CC)C1=CC=C(O)C=C1 CUOJDWBMJMRDHN-VIHUIGFUSA-N 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000009629 microbiological culture Methods 0.000 description 4
- 229960004927 neomycin Drugs 0.000 description 4
- 230000036284 oxygen consumption Effects 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 229920002492 poly(sulfone) Polymers 0.000 description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 229960000268 spectinomycin Drugs 0.000 description 4
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 4
- 229910001220 stainless steel Inorganic materials 0.000 description 4
- 239000010935 stainless steel Substances 0.000 description 4
- 101150118377 tet gene Proteins 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 3
- 241000589516 Pseudomonas Species 0.000 description 3
- 239000003929 acidic solution Substances 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000000035 biogenic effect Effects 0.000 description 3
- 235000010633 broth Nutrition 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000010924 continuous production Methods 0.000 description 3
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 3
- 108010082754 iturin A Proteins 0.000 description 3
- 230000005291 magnetic effect Effects 0.000 description 3
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 3
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 239000003921 oil Substances 0.000 description 3
- 238000012261 overproduction Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 239000001103 potassium chloride Substances 0.000 description 3
- 235000011164 potassium chloride Nutrition 0.000 description 3
- 238000002953 preparative HPLC Methods 0.000 description 3
- 239000012521 purified sample Substances 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 239000008223 sterile water Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000003260 vortexing Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- VLKSXJAPRDAENT-OWGHDAAGSA-N 3-[(3r,6r,9s,16s,19r,22s,25s)-3,9-bis(2-amino-2-oxoethyl)-16-[(1r)-1-hydroxyethyl]-19-(hydroxymethyl)-6-[(4-hydroxyphenyl)methyl]-13-octyl-2,5,8,11,15,18,21,24-octaoxo-1,4,7,10,14,17,20,23-octazabicyclo[23.3.0]octacosan-22-yl]propanoic acid Chemical compound C([C@H]1NC(=O)[C@H](CC(N)=O)NC(=O)CC(NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H]2CCCN2C(=O)[C@@H](CC(N)=O)NC1=O)CCCCCCCC)C1=CC=C(O)C=C1 VLKSXJAPRDAENT-OWGHDAAGSA-N 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 2
- 241000193388 Bacillus thuringiensis Species 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- NZNMSOFKMUBTKW-UHFFFAOYSA-N Cyclohexanecarboxylic acid Natural products OC(=O)C1CCCCC1 NZNMSOFKMUBTKW-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 229930186217 Glycolipid Natural products 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- PCLIMKBDDGJMGD-UHFFFAOYSA-N N-bromosuccinimide Chemical compound BrN1C(=O)CCC1=O PCLIMKBDDGJMGD-UHFFFAOYSA-N 0.000 description 2
- 239000002033 PVDF binder Substances 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 229920001774 Perfluoroether Polymers 0.000 description 2
- 241000223252 Rhodotorula Species 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 241000193624 Wickerhamiella Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- AFVLVVWMAFSXCK-VMPITWQZSA-N alpha-cyano-4-hydroxycinnamic acid Chemical compound OC(=O)C(\C#N)=C\C1=CC=C(O)C=C1 AFVLVVWMAFSXCK-VMPITWQZSA-N 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 229940097012 bacillus thuringiensis Drugs 0.000 description 2
- 230000000443 biocontrol Effects 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 229960005091 chloramphenicol Drugs 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 238000004868 gas analysis Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 108010084762 mycosubtilin synthetase Proteins 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- DCBSHORRWZKAKO-UHFFFAOYSA-N rac-1-monomyristoylglycerol Chemical compound CCCCCCCCCCCCCC(=O)OCC(O)CO DCBSHORRWZKAKO-UHFFFAOYSA-N 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 150000003388 sodium compounds Chemical class 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000004885 tandem mass spectrometry Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- AFVLVVWMAFSXCK-UHFFFAOYSA-N α-cyano-4-hydroxycinnamic acid Chemical compound OC(=O)C(C#N)=CC1=CC=C(O)C=C1 AFVLVVWMAFSXCK-UHFFFAOYSA-N 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- ZQVJBRJGDVZANE-MXDMHAPNSA-N (2s)-2-[(3s,6s,9z,12s,15s,18s,21r,24r,27s)-18,21-bis(2-aminoethyl)-12-benzyl-3-[(1s)-2-chloro-1-hydroxyethyl]-15-[3-(diaminomethylideneamino)propyl]-9-ethylidene-27-[[(3s)-3-hydroxydodecanoyl]amino]-24-(hydroxymethyl)-2,5,8,11,14,17,20,23,26-nonaoxo-1-oxa Chemical compound N1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCN)NC(=O)[C@@H](CCN)NC(=O)[C@@H](CO)NC(=O)[C@@H](NC(=O)C[C@@H](O)CCCCCCCCC)COC(=O)[C@H]([C@H](O)CCl)NC(=O)[C@H]([C@H](O)C(O)=O)NC(=O)\C(=C\C)NC(=O)[C@@H]1CC1=CC=CC=C1 ZQVJBRJGDVZANE-MXDMHAPNSA-N 0.000 description 1
- USPSDZQQNLMVMK-UHFFFAOYSA-N 1-Monolinolein Natural products CCCCCC=CC=CCCCCCCCC(=O)OCC(O)CO USPSDZQQNLMVMK-UHFFFAOYSA-N 0.000 description 1
- WECGLUPZRHILCT-GSNKCQISSA-N 1-linoleoyl-sn-glycerol Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(=O)OC[C@@H](O)CO WECGLUPZRHILCT-GSNKCQISSA-N 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 description 1
- 241000589291 Acinetobacter Species 0.000 description 1
- 102100022089 Acyl-[acyl-carrier-protein] hydrolase Human genes 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000611272 Alcanivorax Species 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 241000186063 Arthrobacter Species 0.000 description 1
- HXMCERBOSXQYRH-KSVGBCIHSA-N Arthrofactin Chemical compound CCCCCCCC1CC(=O)N[C@H](CC(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H]([C@H](C)O)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(O)=O)C(=O)O1 HXMCERBOSXQYRH-KSVGBCIHSA-N 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000304886 Bacilli Species 0.000 description 1
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 1
- 241000498991 Bacillus licheniformis DSM 13 = ATCC 14580 Species 0.000 description 1
- 241001249117 Bacillus mojavensis Species 0.000 description 1
- 241000194103 Bacillus pumilus Species 0.000 description 1
- 101100221537 Bacillus subtilis (strain 168) comK gene Proteins 0.000 description 1
- 241000276408 Bacillus subtilis subsp. subtilis str. 168 Species 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- 241001453380 Burkholderia Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- DWGWCWDDNTVOGF-UHFFFAOYSA-N Cl.Cl.Cl.Cl.Cl.Cl Chemical compound Cl.Cl.Cl.Cl.Cl.Cl DWGWCWDDNTVOGF-UHFFFAOYSA-N 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108010039731 Fatty Acid Synthases Proteins 0.000 description 1
- 241000589565 Flavobacterium Species 0.000 description 1
- 241000028652 Impages bacillus Species 0.000 description 1
- 241000222661 Kurtzmanomyces Species 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 101710152191 Low molecular weight antigen MTB12 Proteins 0.000 description 1
- 239000006391 Luria-Bertani Medium Substances 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- 241000179039 Paenibacillus Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical group N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 108010040201 Polymyxins Proteins 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 241001508466 Pseudomonas cichorii Species 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- 241000589614 Pseudomonas stutzeri Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 241001148199 Pseudomonas tolaasii Species 0.000 description 1
- 241000893045 Pseudozyma Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- 241000893092 Schizonella Species 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- ZQVJBRJGDVZANE-UHFFFAOYSA-N Syringomycin Natural products N1C(=O)C(CCCN=C(N)N)NC(=O)C(CCN)NC(=O)C(CCN)NC(=O)C(CO)NC(=O)C(NC(=O)CC(O)CCCCCCCCC)COC(=O)C(C(O)CCl)NC(=O)C(C(O)C(O)=O)NC(=O)C(=CC)NC(=O)C1CC1=CC=CC=C1 ZQVJBRJGDVZANE-UHFFFAOYSA-N 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- 229930190196 Tolaasin Natural products 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- 241000221566 Ustilago Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 239000012871 anti-fungal composition Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000010936 aqueous wash Methods 0.000 description 1
- 108010066374 arthrofactin Proteins 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 108700021360 bacillomycin L Proteins 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003637 basic solution Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- JYYOBHFYCIDXHH-UHFFFAOYSA-N carbonic acid;hydrate Chemical compound O.OC(O)=O JYYOBHFYCIDXHH-UHFFFAOYSA-N 0.000 description 1
- 101150055766 cat gene Proteins 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011437 continuous method Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- DKAGJZJALZXOOV-UHFFFAOYSA-N hydrate;hydrochloride Chemical compound O.Cl DKAGJZJALZXOOV-UHFFFAOYSA-N 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005040 ion trap Methods 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- UOEFDXYUEPHESS-QMGXLNLGSA-N isopropyl beta-D-galactopyranoside Chemical compound CC(C)O[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O UOEFDXYUEPHESS-QMGXLNLGSA-N 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000028744 lysogeny Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 101150052009 mycC gene Proteins 0.000 description 1
- 101150114277 mycb gene Proteins 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 230000001937 non-anti-biotic effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000000424 optical density measurement Methods 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- CUOJDWBMJMRDHN-UHFFFAOYSA-N plipastatin Chemical compound N1C(=O)C(CCC(N)=O)NC(=O)C2CCCN2C(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)O)NC(=O)C(NC(=O)C(CCCN)NC(=O)C(CCC(O)=O)NC(=O)CC(O)CCCCCCCCCCCCC)CC(C=C2)=CC=C2OC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 CUOJDWBMJMRDHN-UHFFFAOYSA-N 0.000 description 1
- 108010069329 plipastatin Proteins 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 150000003112 potassium compounds Chemical class 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- GGJRAQULURVTAJ-PDBXOOCHSA-N rac-1-alpha-linolenoylglycerol Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(=O)OCC(O)CO GGJRAQULURVTAJ-PDBXOOCHSA-N 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 108010034149 surfactin synthetase Proteins 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 108010078552 syringomycin Proteins 0.000 description 1
- 238000010846 tandem mass spectrometry analysis Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 238000013060 ultrafiltration and diafiltration Methods 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/64—Cyclic peptides containing only normal peptide links
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Sustainable Development (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
参照文献1:(Ongena and Jacques, 2008 Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol. 16, 115-125 [1]
参照文献2:チェコ特許公開公報第20011620号(CZ20011620) [2]
参照文献3:ドイツ特許出願第102005050123号(DE 102005050123) [3]
参照文献4:フランス特許公報第2578552号(FR 2578552) [4]
参照文献5:(Guez et al., 2007. Setting up and modelling of overflowing fed-batch cultures of Bacillus subtilis for the production and continuous removal of lipopeptides. J Biotechnol, 131, 67-75 [5])
参照文献6:(Davis, Lynch and Varley. 1999. The production of surfactin in batch culture by Bacillus subtilis ATCC 21332 is strongly influenced by the conditions of nitrogen metabolism. Enzyme Microb. Technol. 25, 322-329. [6]
参照文献7:国際公開02/26961号公報(WO 0226961)[7]
参照文献8:欧州特許第1320595号公報(EP 1320595)[8]
本発明は、枯草菌の菌株、ミコサブチリン(mycosubtilins)、これらのミコサブチリンを含む組成物、およびこれらのミコサブチリンを得るための方法を提供することによって、前述の要求を正確に満たす。
参照文献9:(Landy et al. 1948. Bacillomycin; an antibiotic from Bacillus subtilis active against pathogenic fungi. Proc. Soc. Exp. Biol. Med. 67, 539-541) [9]
参照文献10:(Guez et al., 2008. Respiration activity monitoring system (RAMOS), an efficient tool to study the influence of the oxygen transfer rate on the synthesis of lipopeptide by Bacillus subtilis. J. Biotechnol. 134, 121-126 [10])
参照文献11:(Remize and Cabassud 2003, A novel bubble-free oxidation reactor: the G/L membrane contactor. Recent progress in method engineering. Integration of membranes in the methods 2. Lavoisier Tec and Doc. [11])
− GEヘルスケアヨーロッパ社(Munich, Germany)からの(CFP-6-D-45)、
− CFPで始まる参照記号によって定義される型式に従ってGEヘルスケアから供給される中空糸濾過モジュール(中空糸のカテゴリ)
− KMで始まる参照記号によって定義される型式に従ってスペクトラムラボから供給される中空糸濾過モジュール(中空糸モジュール)。
(b) ステップ(a)で得られた培地を、その培地から微生物を分離するために精密濾過するステップ、および/または
(c) ステップ(a)または(b)で得られた培地を、ステップ(a)または(b)で得られた培地からバイオサーファクタントを分離するために限外濾過するステップ、を含む。
− 0.2μmの孔径を有する中空ポリスルホン糸または中空ポリエーテルスルホン糸、CFP-2-E-4X2MAを参照(GE-Healthcare Europe GmbH, Munich, Germany)、
− 0.45μmの孔径を有する中空ポリスルホン糸または中空ポリエーテルスルホン糸、CFP-4-E-4X2MAを参照、または、0.65μmの孔径を有する中空ポリスルホン糸または中空ポリエーテルスルホン糸、CFP-2-E-6X2MAを参照(GE-Healthcare Europe GmbH, Munich, Germany)
− CFPで始まる参照記号によって定義される型式に従ってGEヘルスケア社から提供される中空糸精密濾過モジュールまたは中空糸限外濾過モジュール(中空糸カテゴリ)、
− KMで始まる参照記号によって定義される型式に従ってスペクトラムラボ(Spectrum Labs) (Rancho Dominguez, CA, USA)から提供される中空糸濾過モジュール(中空糸モジュール)、
− SPCから始まる参照記号によって定義される型式に従ってザルトリウス・ステディム(Sartorius Stedim)(Aubagne, France)から提供されるSartocon精密濾過カセット。
− 再生セルロースから製造された10kDaの遮断しきい値を有する限外濾過膜、3051443901E-SWを参照(Sartorius, Goettingen, Germany),
− 再生セルロースから製造された10kDaの遮断しきい値を有する限外濾過膜、P2C010C01を参照(Millipore Headquarters, 290 Concord Road, Billerica, MA, USA)。
− ミコサブチリンC18:次の式(I)によって示され、18個の炭素原子を有するミコサブチリンの脂肪酸鎖。
− ミコサブチリンGln3 C17:次の式(II)によって示され、17個の炭素原子を有するとともに、そのペプチド環における3位のアスパラギン(asparagine)に代えてグルタミン(glutamine)を有するミコサブチリンの脂肪酸鎖。
実施例1:枯草菌BBG125株の構築
参照文献12:(Duitman et al, 1999. The mycosubtilin synthetase of Bacillus subtilis ATCC 6633: a multifunctional hybrid between a peptide synthetase, an amino transferase, and a fatty acid synthase. Proc. Natl. Acad. Sci. USA, 96, 13294-13299 [12])
1.1 εpbp-P repU -neo-εfenFおよびrep(R6K)を含むハイブリッドプラスミドpBG200を構築するためのプロトコル
プラスミドpBG106(参照文献13)は、下記(参照文献14)に述べるプロトコルに従って配列番号11の塩基配列の2.6キロ塩基対(kb)のεpbp-PrepU-neo-εfenF断片を分離するとともに精製するために、制限酵素SphI(Fermentas, Villebon sur Yvette, France; reference ER0601)および制限酵素SacI(Fermentas, Villebon sur Yvette, France; reference ER1131)によって消化された。
参考文献13:Lecle're et al, 2005. Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism’s antagonistic and biocontrol activities. Appl. Environ. Microbiol., 71, 4577-4584 [13]
参考文献14:Sambrook and Russell, 2001. Molecular cloning: a laboratory manual, 3rd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York [14]。
参照文献14:(Sambrook and Russell, 2001 [14])
参照文献15:(Dennis and Zylstra, 1998. Plasposons: modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes. Appl. Environ. Microbiol. 64, 2710-2715 [15])
− Sphl: GCATGC(塩基配列の配列番号11の1位)
− Xbal: TCTAGA(塩基配列の配列番号11の743位および2088位)
− Sacl: GAGCTC(塩基配列の配列番号11の2630位)
− カセットεpbp:Sphl から Xbalへ(配列番号12)
− カセットPrepU-neo: Xbal からXbalへ (配列番号13)
− カセットεfenF: XbalからSaclへ (配列番号14)
参照文献16:(Herrero, de Lorenzo and Timmis, 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172: 6557-67 [16])
参照文献17:(Bertani, 2003, Lysogeny at mid-twentieth century: P1, P2 and other experimental systems. J. Bacteriol. 186, 595-600 [17])
枯草菌RFB102株(枯草菌ATCC 6633に由来する菌株)は、amyEの Pspac-comKカセットの挿入によって得られた。Pspacは、IPTGによって誘導され得るプラスミドpA-spac(Bacillus Genetic Stock Center, Columbus, OH, USA)に由来するプロモータを指定し、comKはバチルス属の生来のコンピテンスに対して必須の遺伝子を指定する。これはスペクチノマイシンへの耐性遺伝子(Pspac-comk-spc)に関連しており、染色体遺伝子amyEに統合されている。RFB102株は、IPTG (isopropyl-β-D-galactopyranoside)によって誘導される生来のコンピテンスによって、形質変換のための能力が増進されている。それは、プラスミド増幅システムTempliPhi (GE Healthcare)によって前処理されたpBG200によって形質変換され、その後、Dubnauによって 1982年に記述されたプロトコル(参照文献18)に従ってネオマイシンへの耐性を用いて選択された。
参照文献18:(Genetic transformation of Bacillus subtilis p148-178. In D. Dubnau (Ed) The molecular biology of the Bacilli, vol. I. Bacillus subtilis. Academic Press, Inc. New York [18])
プラスミドpGEM-T Easy(Promega Corp, Charbonnie'res, France)に予め挿入された枯草菌168株(Accession NCBI PRJNA76)、すなわち、枯草菌亜種のゲノムDNAから、プライマーSRF-FO ACAGGAATATGCTCAATCGAAG (配列番号3)およびプライマーSRF-REV AAATTCGCTTCCAGGCTTCTG (配列番号4)を用いて、カセットεsrfAA (2.2 kb)がPCRによって生成された。
(なお、修正E培地は、Clark E培地におけるグルコース濃度を40g/lから20g/lに減少する修正をしたものである。この(修正E)培地の組成は以下である。リン酸二水素カリウム(KH2PO4)2.7g/l、リン酸水素二カリウム(K2HPO4)18.9g/l、酵母エキス0.5g/l、グルコース20g/l、EDTA0.05g/l、硫酸マグネシウム0.61g/l、硫酸マンガン(II)0.056g/l、塩化ナトリウム0.1g/l、塩化カルシウム0.012g/l、硫酸亜鉛0.018g/l、硫酸鉄(II)(FeSO4)0.018g/l、硫酸銅(II)0.002g/l、モリブデン酸ナトリウム0.001g/l、ホウ酸0.001g/l、亜硫酸ナトリウム0.001g/l、塩化ニッケル(II)0.0037g/l、硝酸アンモニウム4g/l、硫酸マグネシウム1g/l。溶液のpHは、10%の塩酸溶液を用いて6.5に調整されている。)
溶液は、その後、渦流によって均質にされた。予め得られた培養液の体積は1.5mlであり、修正E培地を48.5ml含む500mlの三角フラスコ内に加えられた。全体が温度30℃で、120rpmの撹拌下で24時間培養された。この第1の手順P1は複製であった。
なお、nutritive Luria-Bertaniゲロースは(トリプトン(Tryptone)10g/l、酵母エキス5g/l、塩化ナトリウム10g/l、pH7.2)であり、Mosselゲロースは(肉エキス1g/l、ペプトン1g/l、D−マンニトール10g/l、塩化ナトリウム10g/l、フェノールレッド0.025g/l、寒天12g/l、卵黄10ml/l、ポリキシミン(polymyxin)5ml/l、pH7.1)である。
培養液上清、または、ODSカートリッジ(Grace Davison-Alltech, Deerfield, IL USA)で精製されたサンプル、または、ODSカートリッジおよび分取用高速液体クロマトグラフィによって精製されたサンプルが用いられた。
TA緩衝液が、CH3CN/水/トリフルオロ酢酸を体積比33/67/0.1で混合することにより製造されて準備された。CHCA緩衝液は、TA緩衝液におけるα-シアノ-4-ヒドロキシケイ皮酸(alpha-cyano-4-hydroxycinnamic acid)の飽和溶液である。この緩衝液は、α-シアノ-4-ヒドロキシケイ皮酸/TA緩衝液の遠心分離後に上清を回収することにより準備された。解析されるべきサンプルは、サンプル1μlとCHCA緩衝液9μlを混合させて準備された。サンプルの溶液は、MALDI−TOF解析によって0.5μlの体積を示して沈殿した。大気中で乾燥が行われた。質量が標準ペプチド類の混合物によって測定された。
処理前の同位体の質量:M=1098.6
79BR2を用いて処理後の同位体の質量:M=1270.4
断片yは、ミコサブチリンanteiso C17と同一
断片bは、ミコサブチリンanteiso C17の断片bに関して14より大きい
このことは、開放配列中の最初の2つのアミノ酸のうちの1つ(NQPSNvNwのうちのNまたはQ)が、修正されていることを高い確率で意味する。
処理前の同位体の質量:M=1098.6
79Br2を用いて処理後の同位体の質量:M=1270.4
断片yは、ミコサブチリンanteiso C17と同一であるが、14以上を含む。他のサンプル全てが1015のピークであるのに対し、代わりに1029でy6の強烈なピークを含んでいる。
断片bは、ミコサブチリンanteiso C17と同一な断片b6より小さい。
断片bは、14またはそれ以上のミコサブチリンと同一のb6に比べて同等以上。
これらの結果は、対象分子は、C18を有するミコサブチリンというよりは、C17脂肪酸を有するミコサブチリンであろうことを示す。
参照文献19:(Besson et al. 1979. Antifungal activity upon Saccharomyces cerevisiae of iturin A, mycosubtilin, bacillomycin L and of their derivatives; inhibition of this antifungal activity by lipid antagonists. J. Antiobiot. (Tokyo) 32, 828-833 [19])。
− ポンプP1、P2、P3、P4、P5、P6、P7、P8、P9、P10、P11、P12、P13およびP14は、マスターフレックスL/S蠕動ポンプのコンパクト駆動モデル(Masterflex L/S peristaltic pumps compact drive model)、(Cole Parmer, Vernon Hills, IL, U.S.A.))であった。
− ポンプP11は、型式N820.3 FT.18のダイヤフラム式真空ポンプ(KNF Neuberger Laboport, Freiburg, Germany)であった。
− 弁V1、V4およびV5は、ポリテトラフルオロエチレン(PTFE)から作られたストップ弁(W3250Y, Thermo Fisher Scientific, Roskilde, Germany)であった。
− 弁V2、V3、V6 およびV7は、ポリテトラフルオロエチレン(PTFE)から作られた3方向ストップ弁(W3250Z, Thermo Fisher Scientific, Roskilde, Germany)であった。
− タンクを構成する、タンク2およびタンク4は、ナルゲン(Nalgene)タンクであり、高密度ポリエチレンから作られたもので有効容積が4リットルであった(2125-4000 Heavy Duty Bottles, Nalgene, Thermo Fisher Scientific, Roskilde, Germany)。
− タンクを構成する、タンク1、タンク3、タンク5、タンク6およびタンク7は、ナルゲン(Nalgene)タンクであり、高密度ポリエチレンから作られたもので、有効容積が10または20リットルであった(2250 Autoclavable Carboys, Nalgene, Thermo Fisher Scientific, Roskilde, Germany)。
− 計量装置B1、B2、B3、B4、B5、B6およびB7は、Ohaus 社のCKW-55型であった(Ohaus Corporation, Pine Brook, NJ, U.S.A.)。
− 枯草菌の菌株は、枯草菌BBG125株であった。
Europe GmbH, Munich, Germany)によって供給されている商品コードCFP-6-D-45である。これは、2つの区画C1およびC2を含む外部モジュールを構成する。区画C1では、酸素を含む滅菌ガスが循環している。区画C2では、種菌を含む培地が、ポンプP4により付勢された膜に対して、24リットル/h/m2の割合で循環させられている。
− 限外濾過:濃縮物はタンク4へ、マスターフレックスL/S蠕動ポンプP8のコンパクト駆動モデル(Cole Parmer, Vernon Hills, IL, U.S.A.)の制御下で送られる。その後、マスターフレックスL/S蠕動ポンプP9のコンパクト駆動モデル(Cole Parmer, Vernon Hills, IL, U.S.A.)の制御下で、膜M4で精製され、タンク4に集められた。マスターフレックスL/S蠕動ポンプP10のコンパクト駆動モデル(Cole Parmer, Vernon Hills, IL, U.S.A.)は、培地の構成物の残りを、区画8およびタンク5へ移動可能にする。この工程は、タンク4内の含有体積が、タンク4の初期体積の10%に減量されるまで継続される。
なお、タンク5は、高密度ポリエチレンから作られた有効容積が10または20リットルのナルゲン(Nalgene)タンク(2250 Autoclavable Carboys, Nalgene, Thermo Fisher Scientific, Roskilde, Germany)であった。
− 透析濾過:このステップは、限外濾過膜M4を通過する残留物質の通過を容易にするために培養液を希釈する。弁V2を開けてタンク4に、水がタンク4の初期の水準を取り戻すまで加えられる。それから、上記に説明した限外濾過ステップが引き続く。この透析濾過ステップは、連続的に4回行われる。
− メタノール(MeOH)の存在下における限外濾過:透析濾過ステップに引き続いて、メタノールが、タンク6からタンク4へマスターフレックスL/S蠕動ポンプP12のコンパクト駆動モデル(Cole Parmer, Vernon Hills, IL, U.S.A.)および弁V2を介して加えられる。メタノールのこの追加は、この時点でタンク4が70重量%のメタノールを含むように、計量装置B4、B5およびB6によって制御される。それから、限外濾過ステップがすでに述べたように続く。このステップは、ミセルを破壊し、膜M4の孔を通してミコサブチリンのモノマーを通過させるであろう。
なお、タンク6は、高密度ポリエチレンから作られた有効容積が10または20リットルのナルゲン(Nalgene)タンク(2250 Autoclavable Carboys, Nalgene, Thermo Fisher Scientific, Roskilde, Germany)であった。
参照文献20:(Chen, Chen and Juang, 2007. Separation of surfactin from fermentation broths by acid precipitation and two-stage dead-end ultrafiltration processes. J. Membr. Sci. 299, 114-121 [20])
参照文献21:(Chen, Chen, and Juang, 2008. Flux decline and membrane cleaning in cross-flow ultrafiltration of treated fermentation broths for surfactin recovery. Sep. Purif. Technol. 62, 47-55 [21])
− 2つの洗浄が、30℃で30分間、滅菌水3リットルを用いて行われた。これらは、固定されたバイオマスをわずかに分離した。
− 30℃での滅菌水を用いた第2洗浄によって、酸素移動係数を測定することができた。
− 2つの洗浄が50℃で1時間、0.1Mの水酸化ナトリウムの3リットルを用いて行われた。これらは、強く固定されていたバイオマスを分離し、リポペプチドの大部分が脱着された。
− 膜は、その後、50℃で0.5M水酸化ナトリウム溶液を用いて1時間、そしてその後、50℃で100ppmの次亜塩素酸溶液を用いて1時間、さらに、25℃で滅菌水を用いて膜の中が中性になるまで洗浄されることにより、再生された。
− pHは7に維持された。
− 空気/液体の膜接触器の空気流量は、1vvmであった。
− 培地の体積は、3リットルであり、前記膜のファイバーのなかを0.021m/sの速度で循環していた。
− システムの全体の圧力は、空気入口以外は大気圧であり、空気入口は0.4バール大気圧より高かった。
− 培地の酸化条件は、約40h-1の酸素輸送容量係数に固定された。
(gm-2)=(OUR-(X* OURspe cl))*V/(OURspe ci *a)
ここで、
OURは酸素消費率、
OURspe cl は自由細胞に特有の酸素消費率、
OURspe ci は固定された細胞に特有の酸素消費率、
Vは、反応体積、
Xは自由バイオマスの濃度、
aは膜の表面積、をそれぞれ示す。
− 実施例6に記載された内容に従った、前記基質の所定の流量を前記反応装置のなかで連続的に供給するとともに取り除くための装置、
− 実施例6に記載されたことに従って、0.45m2の表面積と0.2μmの孔径を有する精密濾過膜M2、
− 3つのタンク:実施例6に記載された内容に従った、供給(タンク1)、濃縮(タンク2)および排出(タンク3)、および
− 実施例6に記載されたことに従った、0.1m2の表面積と10kDaの遮断しきい値を有する限外濾過膜M3と、が存在する。
Claims (15)
- サーファクチンをコードするためのオペロンsrfAが中断されており、およびミコサブチリンをコードするためのオペロンmycが構成的プロモータPrepUによって置換されていることを特徴とする、枯草菌ATCC 6633株からなる枯草菌株。
- パスツール研究所(フランス・パリ)の国立培養微生物収集機関(CNCM)に番号CNCM I- 445で2011年3月10日に寄託された枯草菌株からなる、請求項1に記載の枯草菌株。
- ミコサブチリンを得るための方法であって、請求項1または2に記載の枯草菌株を培養するステップと、得られた前記ミコサブチリンを回収するステップと、を有する方法。
- (a)有機基材を有する培地のなかで前記枯草菌株を培養するステップであって、前記培養は、空気/液体の膜接触器の表面で行われる、請求項3に記載の方法。
- さらに、
(b) 前記培地から微生物を分離するために、ステップ(a)で得られた培地の精密濾過ステップ、および/または
(c) ステップ(a)または(b)で得られた培地からバイオサーファクタントを分離するために、ステップ(a)または(b)で得られた培地の限外濾過ステップを有する、請求項4に記載の方法。 - 前記有機基材が、でんぷん、グルコース、サッカロース、キシロース、グリセロール、有機酸、アミノ酸、および、これら有機基材の混合物を含む群から選択される、請求項4または5に記載の方法。
- 前記空気/液体の膜接触器は、0.01μmと2μmの間の大きさの孔を有する、請求項4から6のいずれかに記載の方法。
- 前記精密濾過ステップ(b)で使用される中空糸膜が、0.1マイクロメートルから0.45マイクロメートルの遮断しきい値を有する、請求項4から7のいずれかに記載の方法。
- 前記限外濾過ステップ(c)で使用される膜が、5kDaと20kDaとの間の遮断しきい値を有する、請求項4から8のいずれかに記載の方法。
- 請求項10に記載の少なくとも1つのミコサブチリンC18、および/または請求項11に記載の少なくとも1つのミコサブチリンGln3 C17を有する組成物。
- さらに、ミコサブチリンiso-C16、ミコサブチリンn-C16、ミコサブチリンanteiso-C17、ミコサブチリンiso-C17、およびこれらの混合物を含む群から選択される少なくとも1つのミコサブチリンを含む、請求項12に記載の構成物。
- ミコサブチリンiso-C16を1%から60%、ミコサブチリンGln3 C17を1%から20%、ミコサブチリンn-C16を1%から10%、ミコサブチリンanteiso-C17を20%から95%、ミコサブチリンiso-C17を5%から30%、ミコサブチリンC18を1%から5%含む、請求項12または13に記載の組成物。
- 請求項10または11に記載のミコサブチリン、または請求項12から14に記載の組成物であって、抗真菌薬として使用されるミコサブチリンまたは組成物。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1158922A FR2980802B1 (fr) | 2011-10-03 | 2011-10-03 | Procede de production de biosurfactants et dispositif de mise en œuvre |
FR1158922 | 2011-10-03 | ||
PCT/FR2012/052234 WO2013050700A2 (fr) | 2011-10-03 | 2012-10-03 | Biosurfactants de bacillus sp., composition les comprenant, procédé d'obtention et application |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2014528964A true JP2014528964A (ja) | 2014-10-30 |
JP6297493B2 JP6297493B2 (ja) | 2018-03-20 |
Family
ID=47089059
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2014533967A Active JP6297493B2 (ja) | 2011-10-03 | 2012-10-03 | 組成物および抗真菌性組成物 |
Country Status (14)
Country | Link |
---|---|
US (2) | US9688725B2 (ja) |
EP (1) | EP2764126B1 (ja) |
JP (1) | JP6297493B2 (ja) |
CN (2) | CN106867950B (ja) |
AU (1) | AU2012320330C1 (ja) |
BR (1) | BR112014007939B1 (ja) |
CA (1) | CA2851033C (ja) |
ES (1) | ES2666279T3 (ja) |
FR (1) | FR2980802B1 (ja) |
HU (1) | HUE036553T2 (ja) |
PL (1) | PL2764126T3 (ja) |
PT (1) | PT2764126T (ja) |
RU (1) | RU2605625C2 (ja) |
WO (1) | WO2013050700A2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019103491A (ja) * | 2017-12-12 | 2019-06-27 | 水ing株式会社 | 枯草菌の単離方法、その枯草菌、枯草菌を含む微生物製剤、枯草菌単離用培地セット |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX2015015252A (es) * | 2013-05-03 | 2016-06-06 | Univ Eafit | Proceso de produccion de biomasa y metabolitos de microorganismos de la especie bacillus sp. y sus composiciones para el control biologico de plagas. |
CN103865855B (zh) * | 2014-03-25 | 2017-03-29 | 南京工业大学 | 枯草芽孢杆菌菌株及其应用 |
TWI537388B (zh) * | 2014-12-01 | 2016-06-11 | 南京莎菲特生物科技有限公司 | 一種利用高產量枯草桿菌突變株進行半固態發酵生產表面素之方法 |
CN106282041A (zh) * | 2015-05-12 | 2017-01-04 | 南京莎菲特生物科技有限公司 | 一株高产表面素的枯草芽孢杆菌突变株及利用该突变株进行半固态发酵生产表面素的方法 |
CN106260502A (zh) * | 2015-05-12 | 2017-01-04 | 南京莎菲特生物科技有限公司 | 一种制备含表面素饲料添加剂的方法 |
US10576519B2 (en) | 2015-09-10 | 2020-03-03 | Locus Oil Ip Company, Llc | Enhanced microbial production of biosurfactants and other products, and uses thereof |
CN105274045A (zh) * | 2015-10-29 | 2016-01-27 | 山东省科学院中日友好生物技术研究中心 | 枯草芽孢杆菌芽孢和表面活性素同时高产的液体发酵方法 |
WO2018049182A2 (en) | 2016-09-08 | 2018-03-15 | Locus Solutions, Llc | Distributed systems for the efficient production and use of microbe-based compositions |
WO2018107162A1 (en) | 2016-12-11 | 2018-06-14 | Locus Solutions, Llc | Microbial products and their use in bioremediation and to remove paraffin and other contaminating substances from oil and gas production and processing equipment |
FR3061410B1 (fr) * | 2016-12-30 | 2020-07-10 | Lipofabrik | Composition biostimulante de la croissance des plantes obtenue a partir de surnageant de culture de souches bacillus sp. |
MX2019008057A (es) * | 2017-01-06 | 2019-09-10 | Locus Ip Co Llc | Sistemas y metodos novedosos de fermentacion. |
BR112019015100B1 (pt) | 2017-02-07 | 2022-11-29 | Locus Oil Ip Company, Llc | Método para reduzir a viscosidade do óleo e método para a recuperação de óleo de areias petrolíferas |
CN106854669B (zh) * | 2017-02-09 | 2020-04-03 | 南京工业大学 | 一种脂肽类生物表面活性剂的制备方法 |
MX2019009439A (es) | 2017-02-09 | 2019-10-07 | Locus Oil Ip Company Llc | Composiciones y metodos para reducir el sulfuro de hidrogeno y la corrosion de influencia microbiana en el petroleo crudo, el gas natural y en el equipo asociado. |
WO2018191172A1 (en) | 2017-04-09 | 2018-10-18 | Locus Oil Ip Company, Llc | Microbial products and uses thereof to improve oil recovery |
EP3609629A4 (en) | 2017-04-09 | 2020-12-16 | Locus IP Company, LLC | MATERIALS AND PROCESSES FOR MAINTAINING INDUSTRIAL, MECHANICAL AND RESTORATION EQUIPMENT |
CN106978372B (zh) * | 2017-04-26 | 2020-07-17 | 中国科学院海洋研究所 | 海洋芽孢杆菌及其产生脂肽的应用 |
AU2018266546B2 (en) | 2017-05-07 | 2024-06-13 | Locus Ip Company, Llc | Cosmetic compositions for skin health and methods of using same |
WO2018231791A1 (en) | 2017-06-12 | 2018-12-20 | Locus Oil Ip Company, Llc | Remediation of rag layer and other disposable layers in oil tanks and storage equipment |
US10907106B2 (en) | 2017-06-21 | 2021-02-02 | Locus Oil Ip Company, Llc | Treatment for upgrading heavy crude oil |
WO2019067356A1 (en) | 2017-09-27 | 2019-04-04 | Locus Oil Ip Company, Llc | MATERIALS AND METHODS FOR RECOVERING PETROLEUM IN BITUMINOUS SANDS |
EP3694523A4 (en) | 2017-10-13 | 2021-07-07 | Locus IP Company, LLC | METHODS AND SUBSTANCES FOR PREVENTION AND TREATMENT OF NEURODEGENERATIVE DISEASES |
EP3704260A4 (en) | 2017-10-31 | 2021-10-13 | Locus IP Company, LLC | MATRIX FERMENTATION SYSTEMS AND METHODS FOR MANUFACTURING PRODUCTS BASED ON MICROBES |
CN107828687B (zh) * | 2017-11-17 | 2020-09-29 | 中国科学院海洋研究所 | 海洋芽孢杆菌及其应用 |
MX2020006748A (es) | 2017-12-26 | 2020-11-09 | Locus Ip Co Llc | Composiciones de conservacion de alimentos organicos. |
CA3085343A1 (en) | 2017-12-28 | 2019-07-04 | Locus Ip Company, Llc | Oral health composition comprising purified biosurfactants and/or their derivatives |
US20200318051A1 (en) * | 2017-12-28 | 2020-10-08 | Locus Ip Company, Llc | Reactors and Submerged Fermentation Methods for Producing Microbe-Based Products |
CN107988122B (zh) * | 2018-01-16 | 2021-05-07 | 江苏龙蟠科技股份有限公司 | 枯草芽孢杆菌lpb-3、脂肽类生物表面活性剂及生物降解环保型玻璃清洗液 |
AU2019225229A1 (en) | 2018-02-26 | 2020-09-24 | Locus Agriculture Ip Company, Llc | Materials and methods for control of insect pests using entomopathogenic fungi |
WO2019217548A1 (en) | 2018-05-08 | 2019-11-14 | Locus Agriculture Ip Company, Llc | Microbe-based products for enhancing plant root and immune health |
WO2020069172A1 (en) * | 2018-09-28 | 2020-04-02 | Locus Ip Company, Llc | Hybrid solid state-submerged fermentation using a matrix |
BR112021020454A2 (pt) | 2019-04-12 | 2021-12-14 | Locus Ip Co Llc | Tratamentos de pastagem para sequestro de carbono e redução de emissões de gases de efeito estufa produzidas pela pecuária melhorados |
US20220364126A1 (en) * | 2019-11-01 | 2022-11-17 | Locus Ip Company, Llc | Three-Vessel Reactor System for Producing Microbial Biosurfactants and Other Metabolites |
CN110982746B (zh) * | 2019-12-25 | 2022-03-18 | 陕西紫瑞生物科技有限公司 | 芽孢菌株及应用 |
CN113174352B (zh) * | 2021-04-09 | 2022-04-29 | 中国科学院遗传与发育生物学研究所 | 枯草芽孢杆菌hf1突变体及其构建方法与应用 |
CN113980872A (zh) * | 2021-12-08 | 2022-01-28 | 中国矿业大学 | 一株生产脂肽类生物表面活性剂的莫海威芽孢杆菌菌株 |
CN114774497B (zh) * | 2022-04-22 | 2024-02-09 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | 一种利用辣椒秸秆制备脂肽类抗生素iturin A的方法 |
FR3142491A1 (fr) | 2022-11-29 | 2024-05-31 | Lipofabrik | Nouvelles souches de bacillus subtilis produisant de nouvelles isoformes de mycosubtiline |
CN116496357B (zh) * | 2023-06-26 | 2023-09-22 | 南京益纤生物科技有限公司 | 一种伊枯草菌素a抗菌脂肽及其制备方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63264513A (ja) * | 1987-04-22 | 1988-11-01 | Lion Corp | 化粧料 |
JP2002136804A (ja) * | 2000-06-02 | 2002-05-14 | Celgard Inc | 膜接触装置による液体の脱ガス |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2578552B1 (fr) | 1985-03-05 | 1987-05-22 | Inst Fs Rech Expl Mer | Procedes et dispositifs pour produire en continu des biosurfactants |
ES2082039T3 (es) * | 1990-06-22 | 1996-03-16 | Eniricerche Spa | Un mutante de bacillus subtilis y un metodo para producir surfactina utilizando el mutante. |
TW455591B (en) * | 1993-06-08 | 2001-09-21 | Hoechst Ag | Lipopeptides from actinoplanes sp. with pharmacological action, process for their production and the use thereof |
US6103228A (en) | 1997-05-09 | 2000-08-15 | Agraquest, Inc. | Compositions and methods for controlling plant pests |
EP1320595B1 (en) | 2000-09-29 | 2009-09-02 | Showa Denko K.K. | Production process of surfactin |
DE102005050123B4 (de) | 2005-10-18 | 2015-08-20 | Clemens Belter | Verwendung von Zusammensetzungen bei der Tätowierung |
-
2011
- 2011-10-03 FR FR1158922A patent/FR2980802B1/fr active Active
-
2012
- 2012-10-03 WO PCT/FR2012/052234 patent/WO2013050700A2/fr active Application Filing
- 2012-10-03 CA CA2851033A patent/CA2851033C/en active Active
- 2012-10-03 US US14/349,130 patent/US9688725B2/en active Active
- 2012-10-03 BR BR112014007939-0A patent/BR112014007939B1/pt active IP Right Grant
- 2012-10-03 EP EP12779108.5A patent/EP2764126B1/fr active Active
- 2012-10-03 HU HUE12779108A patent/HUE036553T2/hu unknown
- 2012-10-03 JP JP2014533967A patent/JP6297493B2/ja active Active
- 2012-10-03 CN CN201610885087.4A patent/CN106867950B/zh active Active
- 2012-10-03 RU RU2014116248/10A patent/RU2605625C2/ru active
- 2012-10-03 CN CN201280057896.6A patent/CN104114710B/zh active Active
- 2012-10-03 AU AU2012320330A patent/AU2012320330C1/en active Active
- 2012-10-03 PT PT127791085T patent/PT2764126T/pt unknown
- 2012-10-03 PL PL12779108T patent/PL2764126T3/pl unknown
- 2012-10-03 ES ES12779108.5T patent/ES2666279T3/es active Active
-
2017
- 2017-05-18 US US15/599,022 patent/US20170355732A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS63264513A (ja) * | 1987-04-22 | 1988-11-01 | Lion Corp | 化粧料 |
JP2002136804A (ja) * | 2000-06-02 | 2002-05-14 | Celgard Inc | 膜接触装置による液体の脱ガス |
Non-Patent Citations (2)
Title |
---|
APPL. ENVIRON. MICROBIOL., 2005, VOL. 71, NO. 8, PP. 4577-4584, JPN6016022550 * |
APPL. ENVIRON. MICROBIOL., 2009, VOL. 75, NO. 13, PP. 4636-4640, JPN6016022549 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2019103491A (ja) * | 2017-12-12 | 2019-06-27 | 水ing株式会社 | 枯草菌の単離方法、その枯草菌、枯草菌を含む微生物製剤、枯草菌単離用培地セット |
Also Published As
Publication number | Publication date |
---|---|
US20150045290A1 (en) | 2015-02-12 |
CN104114710B (zh) | 2016-10-26 |
AU2012320330C1 (en) | 2017-03-09 |
FR2980802B1 (fr) | 2014-12-26 |
CN106867950B (zh) | 2022-02-18 |
CN104114710A (zh) | 2014-10-22 |
ES2666279T3 (es) | 2018-05-03 |
US20170355732A1 (en) | 2017-12-14 |
RU2014116248A (ru) | 2015-11-10 |
FR2980802A1 (fr) | 2013-04-05 |
WO2013050700A3 (fr) | 2013-12-19 |
EP2764126A2 (fr) | 2014-08-13 |
AU2012320330B2 (en) | 2015-09-24 |
AU2012320330A1 (en) | 2014-05-01 |
US9688725B2 (en) | 2017-06-27 |
BR112014007939B1 (pt) | 2021-11-23 |
RU2605625C2 (ru) | 2016-12-27 |
PT2764126T (pt) | 2018-03-06 |
HUE036553T2 (hu) | 2018-08-28 |
CN106867950A (zh) | 2017-06-20 |
CA2851033A1 (en) | 2013-04-11 |
WO2013050700A2 (fr) | 2013-04-11 |
BR112014007939A2 (pt) | 2017-04-04 |
PL2764126T3 (pl) | 2018-08-31 |
JP6297493B2 (ja) | 2018-03-20 |
EP2764126B1 (fr) | 2017-11-29 |
CA2851033C (en) | 2018-07-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6297493B2 (ja) | 組成物および抗真菌性組成物 | |
Chen et al. | Applications of a lipopeptide biosurfactant, surfactin, produced by microorganisms | |
Shaligram et al. | Surfactin–a review on biosynthesis, fermentation, purification and applications | |
Bouassida et al. | Improved biosurfactant production by Bacillus subtilis SPB1 mutant obtained by random mutagenesis and its application in enhanced oil recovery in a sand system | |
Manivasagan et al. | Optimization, production and characterization of glycolipid biosurfactant from the marine actinobacterium, Streptomyces sp. MAB36 | |
Al-Ajlani et al. | Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium | |
WO2017044953A1 (en) | Enhanced microbial production of biosurfactants and other products, and uses thereof | |
Lo Cantore et al. | Biological characterization of white line-inducing principle (WLIP) produced by Pseudomonas reactans NCPPB1311 | |
Zeng et al. | A Streptomyces globisporus strain kills Microcystis aeruginosa via cell-to-cell contact | |
Marchant et al. | Production of biosurfactants from nonpathogenic bacteria | |
JP2021506575A (ja) | 廃水中の有機化合物の量の削減のための継続的方法 | |
US20140308733A1 (en) | Method for inducing germination of spore forming bacterium | |
US20200396991A1 (en) | Production of mel-like glycolipids and lipopeptides using a bacillus sp. microorganism | |
Olmedo et al. | Antifungal potential of biosurfactants produced by strains of Bacillus spp.(Bacillales: Bacillaceae) selected by molecular screening | |
KR101728605B1 (ko) | 신규 미생물 | |
Zobaer et al. | Isolation of biosurfactant producing bacteria from oil-spilled soil and characterization of their secreted biosurfactants in pathogen-inhibition and oil-emulsification | |
Patel | Isolation and characterization of detergent degrading bacteria from soil | |
Mulango et al. | Isolation and characterization of haloalkaliphilic bacteria from the hot springs of Lake Magadi | |
RU2492231C2 (ru) | Способ выделения бактериоцинов | |
Prabakaran et al. | Research Article Studies On The Biosurfactant Producing Pseudomonas Aeruginosa And Its Antimicrobial Activity On Selected Human Pathogens | |
DAS et al. | In vitro antimicrobial activity and molecular characterization of Bacillus amyloliquefaciens isolated from similipal biosphere reserve, Odisha, India | |
Denich et al. | Fluorescent methods to study DNA, RNA, proteins and cytoplasmic membrane polarization in the pentachlorophenol-mineralizing bacterium Sphingomonas sp. UG30 during nutrient starvation in water | |
Gagik et al. | METABOLIC AND GENETICAL FEATURES OF BIODEGRADATION AND RESISTANCE POTENTIAL OF SOIL PSEUDOMONAS SP. FROM THE NATIONAL CULTURE COLLECTION OF MICROORGANISMS, REPUBLIC OF ARMENIA | |
Donio et al. | Antagonistic Bacillus cereus TC-1 Isolated from Solar Salt Work in Southern India | |
Muhyaddin et al. | Isolation and identification of antifungal substances producer Bacillus |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20150715 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20160621 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160920 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20161121 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20170411 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20170710 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20170908 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20180206 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20180221 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6297493 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |