JP2014518875A - 特定のキトサン系ナノ複合体を用いてsiRNAを効果的かつ安全に送達するための組成物及び方法 - Google Patents

特定のキトサン系ナノ複合体を用いてsiRNAを効果的かつ安全に送達するための組成物及び方法 Download PDF

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JP2014518875A
JP2014518875A JP2014511695A JP2014511695A JP2014518875A JP 2014518875 A JP2014518875 A JP 2014518875A JP 2014511695 A JP2014511695 A JP 2014511695A JP 2014511695 A JP2014511695 A JP 2014511695A JP 2014518875 A JP2014518875 A JP 2014518875A
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cancer
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chitosan
nucleic acid
rnai
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アブデラザク メルゾーキ
マイケル ディー ブッシュマン
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ポリヴァロール ソシエテ アン コマンディト
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JP2014511695A 2011-05-24 2012-05-24 特定のキトサン系ナノ複合体を用いてsiRNAを効果的かつ安全に送達するための組成物及び方法 Pending JP2014518875A (ja)

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US201161489306P 2011-05-24 2011-05-24
US201161489302P 2011-05-24 2011-05-24
US61/489,306 2011-05-24
US61/489,302 2011-05-24
PCT/CA2012/050342 WO2012159215A1 (en) 2011-05-24 2012-05-24 Compositions and methods for efficacious and safe delivery of sirna using specific chitosan-based nanocomplexes

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JP2014518875A true JP2014518875A (ja) 2014-08-07
JP2014518875A5 JP2014518875A5 (enExample) 2015-07-09

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Cited By (1)

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WO2017078054A1 (ja) * 2015-11-04 2017-05-11 株式会社ステリック再生医科学研究所 RNAi分子とN-アセチル化キトサンとを含む複合体

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ES2918452T3 (es) 2007-02-19 2022-07-15 Marine Polymer Tech Inc Composiciones hemostáticas y regímenes terapéuticos
US20150025017A1 (en) * 2012-02-28 2015-01-22 Fred Hutchinson Cancer Research Center Compositions and methods for treating cancer
KR101601035B1 (ko) * 2013-02-28 2016-03-08 주식회사 종근당 키토산 및 액상결정 형성 물질을 포함하는 유전자 전달용 조성물
JP6300212B2 (ja) * 2013-03-21 2018-03-28 国立大学法人 東京医科歯科大学 二重鎖核酸結合剤、当該結合剤−二重鎖核酸複合体、当該複合体を含有する医薬品組成物、及び、当該複合体の製造方法
CA2915131A1 (en) * 2013-06-10 2014-12-18 Polyvalor, Societe En Commandite Freeze-dried polyelectrolyte complexes that maintain size and biological activity
WO2016138132A1 (en) * 2015-02-25 2016-09-01 The Trustees Of Columbia University In The City Of New York Dipeptidyl peptidase-iv(dpp4) inhibitors, methods and compositions for suppressing adipose tissue inflammation
BR112018071997A2 (pt) 2016-04-26 2019-02-12 Viaqua Therapeutics Ltd nanopartícula de quitosana-rna, nanopartícula de quitosana-dsrna, composição nutracêutica, crustáceo criado em cativeiro, e método de tratamento ou prevenção de uma doença ou condição em um crustáceo criado em cativeiro associado a um vírus patogênico
JP7179715B2 (ja) * 2016-07-27 2022-11-29 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 核酸送達用の崩壊性細胞透過性複合体
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WO2019226612A1 (en) * 2018-05-21 2019-11-28 The United States Government As Represented By The Department Of Veterans Affairs Compositions and methods for treating rsv-infections
EP3586851A1 (en) * 2018-06-27 2020-01-01 Nextraresearch S.r.l. Compositions for oral administration of pentosan polysulfate and chitosan in form of nanoparticles with improved intestinal absorption
CN110396524B (zh) * 2019-05-31 2023-05-02 海南大学 一种用于蚊虫的RNAi纳米颗粒和制备方法及用途
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CN115748011B (zh) * 2022-11-16 2024-06-25 中国科学技术大学 检测miRNA-21的荧光壳聚糖纤维的制备方法及检测miRNA-21的方法
CN118304423B (zh) * 2023-05-25 2024-10-11 无锡市第二人民医院 多层级壳聚糖明胶颗粒载P4HA1 siRNA通过抑制细胞EMT治疗脑胶质瘤的研究
CN118615495B (zh) * 2024-05-08 2026-03-31 山东大学 用于骨肉瘤术后防复发与促进骨修复的凝胶及其制备方法与应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007059605A1 (en) * 2005-11-04 2007-05-31 Bio Syntech Canada Inc. Composition and method for efficient delivery of nucleic acids to cells using chitosan
WO2008020318A2 (en) * 2006-03-30 2008-02-21 Engene, Inc. Non-viral compositions and methods for transfecting gut cells in vivo
JPWO2006054625A1 (ja) * 2004-11-19 2008-05-29 株式会社ジーンケア研究所 癌細胞特異的細胞増殖抑制剤
JP2009530319A (ja) * 2006-03-23 2009-08-27 サンタリス ファーマ アー/エス 低分子内部セグメント化干渉rna
US20100015232A1 (en) * 2006-07-07 2010-01-21 Aarhus Universitet Nanoparticles for nucleic acid delivery

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1326630E (pt) * 2000-09-18 2008-09-02 Sanos Bioscience As Utilização de péptidos glp-2
US20030171310A1 (en) * 2001-02-23 2003-09-11 Isis Pharmaceuticals Inc. Antisense modulation of RECQL expression
US20050153914A1 (en) * 2001-05-18 2005-07-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MDR P-glycoprotein gene expression using short interfering nucleic acid (siNA)
CA2518475C (en) * 2003-03-07 2014-12-23 Alnylam Pharmaceuticals, Inc. Irna agents comprising asymmetrical modifications

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2006054625A1 (ja) * 2004-11-19 2008-05-29 株式会社ジーンケア研究所 癌細胞特異的細胞増殖抑制剤
WO2007059605A1 (en) * 2005-11-04 2007-05-31 Bio Syntech Canada Inc. Composition and method for efficient delivery of nucleic acids to cells using chitosan
JP2009530319A (ja) * 2006-03-23 2009-08-27 サンタリス ファーマ アー/エス 低分子内部セグメント化干渉rna
WO2008020318A2 (en) * 2006-03-30 2008-02-21 Engene, Inc. Non-viral compositions and methods for transfecting gut cells in vivo
US20100015232A1 (en) * 2006-07-07 2010-01-21 Aarhus Universitet Nanoparticles for nucleic acid delivery

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6016006710; Oligonucleotides vol.20, no.4, 2010, p.183-190 *
JPN6016006711; International Journal of Nanomedicine vol.5, no.1, 20100703, p.473-481 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017078054A1 (ja) * 2015-11-04 2017-05-11 株式会社ステリック再生医科学研究所 RNAi分子とN-アセチル化キトサンとを含む複合体
JPWO2017078054A1 (ja) * 2015-11-04 2018-08-30 株式会社ステリック再生医科学研究所 RNAi分子とN−アセチル化キトサンとを含む複合体
US10646579B2 (en) 2015-11-04 2020-05-12 Tme Therapeutics Inc. Complex comprising RNAi molecule and N-acetylated chitosan
JP2020079270A (ja) * 2015-11-04 2020-05-28 株式会社ステリック再生医科学研究所 RNAi分子とN−アセチル化キトサンとを含む複合体
JP2022068371A (ja) * 2015-11-04 2022-05-09 株式会社ステリック再生医科学研究所 RNAi分子とN-アセチル化キトサンとを含む複合体
JP7424661B2 (ja) 2015-11-04 2024-01-30 株式会社ステリック再生医科学研究所 RNAi分子とN-アセチル化キトサンとを含む複合体

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