JP2014501278A - ルテイン含有シルクエキスを抽出する方法 - Google Patents
ルテイン含有シルクエキスを抽出する方法 Download PDFInfo
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- JP2014501278A JP2014501278A JP2013547406A JP2013547406A JP2014501278A JP 2014501278 A JP2014501278 A JP 2014501278A JP 2013547406 A JP2013547406 A JP 2013547406A JP 2013547406 A JP2013547406 A JP 2013547406A JP 2014501278 A JP2014501278 A JP 2014501278A
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- Prior art keywords
- lutein
- solution
- silk
- aqueous phase
- dry residue
- Prior art date
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- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 229910000406 trisodium phosphate Inorganic materials 0.000 description 1
- 235000019801 trisodium phosphate Nutrition 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
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Abstract
【選択図】図1
Description
ルテイン抽出方法100の第1の適用例では、シルクコクーンのサンプル(1±0.0002g)を、30mLの蒸留水によって、121℃で15分間精練した。その精練したコクーン(P1)を、0.1%のブチル化ヒドロキシトルエン(BHT)(w/v)を含む、30mLのヘキサン、エタノール、及び酢酸エチル(3:2:1、v/v/v)により、無色になるまで、4回抽出した。色素セリシン溶液(P2)のため、分割する前に、同じ抽出溶媒60mLを使用して、褐色ガラス分液漏斗で抽出を行ない、上部の相を得た。そして、上部の相を、真空下で乾燥するまで蒸発させ、乾固残渣が残った。乾固残渣は、HPLCグレードのn−ヘキサン及び酢酸エチル(3:1、v/v)に溶解し、0.45μmのフィルター膜でろ過される前に、5mLの最終量になるように調節した。そして、ろ過後の溶液は、後の分析のため、−20℃の窒素ガス内で保持された。精練したコクーンから抽出したカロテノイド、及び色素セリシン溶液は、HPLCにより同定された。色素エキス内のカロテノイド及びルテインの吸収スペクトラム及び濃度は、分光光度計を用い、エタノールにおいて測定された。
ルテイン抽出方法100の第2の最適例では、色素セリシン複合体は、5つの異なる処理により、黄色シルクコクーンから分離した。この5つの異なる処理とは、121℃下で、黄色シルクコクーンを15分間、30分間、60分間、90分間、120分間続いて精練することを含む。それぞれの処理において、黄色シルクコクーンは、暗所において、1:30の比例で、脱イオン水によって精練された。処理のためのそれぞれの持続時間の経過後、混合物を室温まで冷却し、精練溶液を分離した。脱イオン水を使用して、異なる処理から得た各々の色素セリシン溶液を、100mLの最終容積に調整した。すべての処理は3回行われた。色素セリシン溶液内の総タンパク質値は、ビシンコニン酸(BCA)タンパク質分析キットを用いて測定された。分析は、牛血清アルブミン(BSA)を標準品として、37℃で、30分間行われた。
ルテイン抽出方法100を用い、高温下、様々な時間分で、ルテインを抽出した。図9a〜9cは、(A)有機溶媒により抽出したルテインのクロマトグラム、(B)高速抽出器により100barの圧力下、100℃で、10分間抽出したルテインのクロマトグラム、(C)高速抽出器により100barの圧力下、100℃で、30分間抽出したルテインのクロマトグラム、(D)高速抽出器により100barの圧力下、150℃で、10分間抽出したルテインのクロマトグラムを示している。クロマトグラムの結果は、C18RP‐HPLCによって分析され、移動相としてアセトニトリル/メタノール(9:1,volume/volume)、及び酢酸エチルが用いられた。高温高圧におけるルテインの抽出(ルテイン抽出方法100の溶媒を用いた)は、高温高圧が、ルテインエキスを弱めない、または、損傷しないということが観察された。
ルテイン抽出方法から得られたルテインの効果は、ヒト網膜色素上皮(RPE)細胞培養で試験された。RPE細胞株、ARPE‐19(American Type Culture Collection、ATCC)は、DMEM/F‐12細胞培地にて、ウシ胎児血清(FBS)10%並びにペニシリン/ストレプトマイシンを用いて、二酸化炭素5%細胞培養器内で、37℃で培養された(トリプシン/エチレンジアミン四酢酸(EDTA)溶液による二次培養にて、3日〜4日毎に、細胞培地の変更あり)。結果は図10に示される。
マウスを使って、ルテイン抽出方法100を用いて黄色シルクコクーンから抽出したルテインと、他の原料からのルテインとの、比較効果を研究した。マウスは、一般的に、カロテノイド群の免疫調節効果の研究に用いられる実験動物である。この研究には、National Laboratory Animal Center of Mahidol University(タイ、バンコク)からの生後7週間のメスBALB/cマウスが使用され、Laboratory Animal Center of Faculty of Medical Science Naresuan University(タイ、ピトサヌロク(Phitsanulok))において、照明制御された部屋で、12時間:12時間の明暗サイクルで、25±1℃にて行われた。マウスは、この実験のため、滅菌蒸留水を与えた。
グループ1は、ルテインの溶媒のみをマウスに与え(PBS pH7.4の中に1% Tween80、溶剤対象群)、
グループ2は、黄色コクーンから抽出したルテインを、10mg/kg体重で、マウスに与え(sLT10)、
グループ3は、黄色コクーンから抽出したルテインを、20mg/kg体重で、マウスに与え(sLT20)、
グループ4は、マリーゴールドの花から抽出した市販のルテイン(キサントフィル≧95%、中国製)を、10mg/kg体重で、マウスに与え(cLT10)、
グループ5は、マリーゴールドの花から抽出した市販のルテイン(キサントフィル≧95%、中国製)を、20mg/kg体重で、マウスに与えた(cLT20)。
Claims (17)
- ルテインを抽出する方法であって、
シルク繊維を、ヘキサン、エチルアルコール及び酢酸エチルが含まれる複数の溶媒と接触させて第1の溶液を得るステップと、
前記第1の溶液を非水相及び水相に分割するステップと、
前記非水相を乾燥させて乾固残渣を得るステップと、
前記乾固残渣を複数の溶媒のうちの1つ以上の溶媒に溶解させて第2の溶液を得るステップと、
前記第2の溶液をろ過して前記第2の溶液からルテインエキスを得るステップと、
を含む方法。 - 請求項1に記載の方法において、
シルクコクーン、シルク糸及びシルク廃棄物のうちの少なくとも1つを精練してシルク繊維を得るステップ、
をさらに含む方法。 - 請求項2に記載の方法において、
前記シルクコクーン、シルク糸及びシルク廃棄物のうちの少なくとも1つを精練するステップは、
前記シルクコクーン、シルク糸及びシルク廃棄物のうちの少なくとも1つを水に浸漬して、精練溶液を得るステップと、
精練溶液を除去してシルク繊維を得るステップと、
を含む方法。 - 請求項3に記載の方法において、
前記シルクコクーン、シルク糸及びシルク廃棄物のうちの少なくとも1つを精練するステップは、
水に浸漬した前記シルクコクーン、シルク糸及びシルク廃棄物のうちの少なくとも1つを加熱するステップ、
をさらに含む方法。 - 請求項1に記載の方法において、
前記シルク繊維を複数の溶媒と接触させるステップは、
前記シルク繊維を複数の溶媒と接触させることによって、前記シルク繊維から複数の有機溶液部分を抽出するステップと、
前記複数の有機溶液をプールして第1の溶液を得るステップと、
を含む方法。 - 請求項1に記載の方法において、
前記第1の溶液を分割するステップは、
含水塩化ナトリウムを第1の溶液に添加するステップ、
を含む方法。 - 請求項1に記載の方法において、
前記非水相を乾燥させるステップは、
非水相から水相を分離するステップと、
無水硫酸ナトリウムを分離した非水相に添加するステップと、
を含む方法。 - 請求項7に記載の方法において、
前記水相を乾燥させるステップは、
分離した水相を蒸発させて乾燥させるステップ、
をさらに含む方法。 - 請求項1に記載の方法において、
前記乾固残渣を溶解させるステップは、
前記乾固残渣をヘキサン及び酢酸エチルに溶解させるステップ、
を含む方法。 - 請求項1に記載の方法において、
シリンジフィルターによって第2の溶液をろ過する方法。 - ルテインを抽出する方法であって、
シルクコクーンを精練してシルク繊維を得るステップと、
前記シルク繊維を、ヘキサン、エチルアルコール及び酢酸エチルが含まれる1つ以上の溶媒と接触させて第1の溶液を得るステップと、
前記第1の溶液を非水相及び水相に分割するステップと、
前記非水相を乾燥させて乾固残渣を得るステップと、
前記乾固残渣をヘキサン及び酢酸エチルに溶解させて第2の溶液を得るステップと、
前記第2の溶液をろ過して前記第2の溶液からルテインエキスを得るステップと、
を含む方法。 - 請求項11に記載の方法において、
前記第1の溶液を分割するステップは、
含水塩化ナトリウムを第1の溶液に添加するステップ、
を含む方法。 - 請求項11に記載の方法において、
前記水相を乾燥させるステップは、
非水相から水相を分離するステップと、
無水硫酸ナトリウムを分離した非水相に添加するステップと、
を含む方法。 - 請求項13に記載の方法において、
前記非水相を乾燥させるステップは、
分離した非水相を蒸発させて乾燥させるステップ、
をさらに含む方法。 - 請求項11に記載の方法において、
前記乾固残渣を溶解させるステップは、
前記乾固残渣をヘキサン及び酢酸エチルに溶解させるステップ、
を含む方法。 - 請求項11に記載の方法において、
シリンジフィルターによって第2の溶液をろ過する方法。 - ルテインを抽出する方法であって、
シルク繊維を、ヘキサン、エチルアルコール及び酢酸エチルが含まれる1つ以上の溶媒と接触させて第1の溶液を得るステップと、
前記第1の溶液を非水相及び水相に分割するステップと、
前記非水相を乾燥させて乾固残渣を得るステップと、
前記乾固残渣を1つ以上の溶媒に溶解させて第2の溶液を得るステップと、
前記第2の溶液をろ過して前記第2の溶液からルテインエキスを得るステップと、
を含む方法。
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US20050038271A1 (en) * | 2001-11-29 | 2005-02-17 | Frederick Khachik | Process for extraction and purification of lutein, zeaxanthin and rare carotenoids from marigold flowers and plants |
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CN101514177A (zh) * | 2009-03-13 | 2009-08-26 | 沈阳药科大学 | 从万寿菊颗粒中提取叶黄素的方法 |
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US9018422B2 (en) | 2015-04-28 |
AU2010366644B2 (en) | 2017-04-20 |
WO2012091683A1 (en) | 2012-07-05 |
KR20140004674A (ko) | 2014-01-13 |
AU2010366644A1 (en) | 2013-07-11 |
EP2658836A1 (en) | 2013-11-06 |
US20130331615A1 (en) | 2013-12-12 |
SG191379A1 (en) | 2013-08-30 |
JP5767716B2 (ja) | 2015-08-19 |
EP2658836A4 (en) | 2015-12-30 |
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