JP2014204698A - Air supply system, and microorganism culture apparatus including the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an air supply system and a microorganism culture apparatus including the system which are capable of surely preventing occurrence of clogging in an air feeding pipe or the like.SOLUTION: An air supply system B for supplying gas G into liquid 1 containing soluble substance comprises: an air feeding pipe 4 which is disposed such that the inside of the pipe is communicated into the liquid 1; air feeding means 5 for circulating the gas G inside the air feeding pipe 4; and clogging preventing liquid supply means 7 for supplying clogging preventing liquid 8 into the air feeding pipe 4.

Description

  The present invention relates to an air supply system and a microorganism culture apparatus equipped with the same.

  For example, it has been noted that microorganisms such as Chlorella, Chlamydomonas, Donariella, Senedesmus, Synecococcus, and Spirulina microalgae are used for foods and fuels (see, for example, Patent Document 1). In general, an apparatus for cultivating this type of microorganism is used by supplying air from the bottom side of the culture tank by an air supply system to aerate the culture solution, and stirring the culture solution with a stirring blade. It is configured to supply oxygen and nutrient sources in sufficient contact with microorganisms in the liquid.

  On the other hand, when a large amount of microorganisms such as microalgae are cultured as described above, the microorganisms adhere to the inner wall of the culture tank or the structure inside the culture tank, or agglomerates of microorganisms are formed and settled. In many cases, the culture efficiency is reduced. On the other hand, when cultivating microorganisms, a technique has been proposed and put into practical use to prevent microorganisms from adhering to the inside of the culture tank and sedimentation due to the formation of agglomerates by intermittently applying ultrasonic waves to the culture tank. (For example, refer to Patent Document 2).

JP 2012-239423 A JP 7-298869 A

  Here, for example, when cultivating halophilic microorganisms, the salinity (dissolved substance dissolved in the liquid) in the culture solution causes the air supply system air pipes, air stones, aeration balls (aeration means), etc. It is deposited / adhered to the air and its air supply capacity is lowered. In particular, when cultivating moderately halophilic microorganisms that grow favorably at a salinity of 0.5 to 2.5M, and highly halophilic microorganisms that favorably grow at a salinity of 2.5M or more, The higher the salinity concentration, the higher the rate of decrease in the air supply capacity, and the shorter the time until the air supply capacity decreases.

  Further, it is difficult to sufficiently remove solid substances such as salt deposited / adhered to an air supply pipe or an air stone of such an air supply system even if ultrasonic waves are used. As a result, conventionally, there has been a problem in that it is necessary to take measures to replace the parts of the air supply system when the air supply capacity is reduced.

  In view of the above circumstances, an object of the present invention is to provide an air supply system that can reliably prevent clogging of an air supply pipe and the like, and a microorganism culture apparatus including the air supply system.

  In order to achieve the above object, the present invention provides the following means.

  An air supply system according to the present invention is an air supply system for supplying a gas into a liquid containing a soluble substance, the air supply pipe being arranged in communication with the liquid, and the air supply pipe. It is characterized by comprising air supply means for circulating gas inside and clogging prevention liquid supply means for supplying clogging prevention liquid inside the air supply pipe.

  In the present invention, by providing the clogging prevention liquid supply means, it becomes possible to distribute the clogging prevention liquid together with the gas inside the air supply pipe so as to be supplied and discharged into the liquid. Then, not only the gas but also the liquid for preventing clogging flows through the inside of the air supply pipe as in the conventional case, so that the soluble substance contained in the liquid is deposited / attached inside the air supply pipe of the air supply system. Can be prevented.

  In the air supply system of the present invention, the clogging prevention liquid supply means includes a clogging prevention liquid storage tank for storing clogging prevention liquid, and the inside of the air supply pipe is formed by the air supply means. The flowing gas is supplied into the clogging prevention liquid in the clogging prevention liquid storage tank, and the gas containing the clogging prevention liquid is supplied from the clogging prevention liquid storage tank to the inside of the air supply pipe. It is desirable to be configured to be circulated and fed into the liquid.

  In this invention, the gas flowing through the inside of the air supply pipe is supplied into the clogging prevention liquid in the clogging prevention liquid storage tank by an air supply means such as an air supply pump, thereby clogging the gas. A liquid for prevention can be included. For example, the humidity of the air can be increased by circulating gaseous air through the clogging-preventing liquid water. As a result, it becomes possible to distribute the clogging prevention liquid together with the gas inside the air supply pipe and supply / release it into the liquid, so that the soluble substances contained in the liquid are contained inside the air supply pipe of the air supply system. Can be prevented from depositing / adhering to the surface.

  Furthermore, in the air supply system of the present invention, the clogging prevention liquid supply means includes liquid supply means for supplying the clogging prevention liquid to the inside of the air supply pipe, and the gas is supplied together with the clogging prevention liquid. It is more desirable that the liquid is supplied to the liquid by circulating it in the air pipe.

  In the present invention, the liquid for clogging is supplied from the liquid supply means to the inside of the air supply pipe, whereby the liquid for clogging prevention (clogging prevention liquid) is circulated along with the gas inside the air supply pipe. It is possible to supply and discharge inside. As a result, it is possible to prevent the soluble substance contained in the liquid from being deposited / attached to the inside of the air supply pipe of the air supply system.

  Further, in the air supply system of the present invention, the clogging prevention liquid supply means supplies the clogging prevention liquid so that the clogging prevention liquid is contained at 0.025% by volume or more with respect to the gas. It is further desirable to supply the inside of the air pipe.

  In this invention, by supplying the clogging prevention liquid so that it becomes 0.025 volume% or more with respect to the amount of gas flowing through the inside of the air supply pipe, the soluble substance contained in the liquid is reliably supplied. It is possible to prevent deposition / adhesion inside the air pipe of the air system.

  The microorganism culturing apparatus of the present invention is a microorganism culturing apparatus for culturing microorganisms in a culture solution stored in a culture tank, and any of the above as an air supply system for supplying a gas containing oxygen into the culture solution It is characterized by having such an air supply system.

  In this invention, in order to supply a gas containing oxygen, such as air, into a culture solution for culturing microorganisms, any one of the above-described air supply systems is used to deposit inside the air supply pipe of the air supply system. / Can be prevented from adhering, and microorganisms can be suitably cultured.

  Moreover, in the microorganism culture apparatus of this invention, it is desirable that it is an apparatus which culture | cultivates a halophilic microorganism using the said culture solution containing salt.

  In the present invention, salt deposits / adheres to the inside of the air supply pipe of the air supply system, and conventionally, there is no means for preventing / removing this salt deposition / adhesion, and the air supply capacity is maintained over a long period of time. By applying one of the above-mentioned air supply systems to a halophilic microorganism culture apparatus that has been impossible to prevent, salt deposits / adheres to the inside of the air supply pipe of the air supply system reliably and effectively. This can be prevented, and culturing can be suitably performed for halophilic microorganisms.

  In the air supply system of the present invention and the microorganism culturing apparatus equipped with the same, the air supply system includes clogging prevention liquid supply means, whereby the clogging prevention liquid and the gas are circulated inside the air supply pipe. Thus, it can be supplied and discharged into the liquid. Then, not only the gas but also the liquid for preventing clogging flows through the inside of the air supply pipe as in the conventional case, so that the soluble substance contained in the liquid is deposited / attached inside the air supply pipe of the air supply system. Can be prevented.

  As a result, for example, this air supply system can be reliably and effectively applied to culture of halophilic microorganisms that need to supply a gas containing oxygen such as air to a culture solution having a high salinity concentration. In addition, salt can be prevented from depositing / adhering to the inside of the air supply pipe of the air supply system, and culturing can be suitably performed even for halophilic microorganisms.

It is a figure which shows the air supply system and microorganism culture apparatus which concern on 1st Embodiment of this invention. It is a figure which shows the air supply system and microorganism culture apparatus which concern on 2nd Embodiment of this invention. It is a figure which shows the result of the verification experiment which confirmed the predominance of the air supply system which concerns on this invention. It is a figure which shows the result of the verification experiment which confirmed the predominance of the air supply system which concerns on this invention.

  Hereinafter, an air supply system according to an embodiment of the present invention and a microorganism culture apparatus including the same will be described with reference to FIGS. 1 to 4.

Here, the present embodiment relates to a microbial culture apparatus for culturing halophilic microorganisms that do not grow under low salinity conditions, or that do not grow well and grow well in a culture solution with high salinity. In particular, the present invention relates to a microorganism culture apparatus provided with an air supply system that makes it possible to supply a gas (air) containing oxygen into a culture solution while preventing clogging due to salt.
The air supply system according to the present invention need not be limited to application to the microorganism culture apparatus as in the present embodiment, and can be used in any case where it is necessary to supply a gas to a liquid containing a soluble substance. It is applicable, and it is possible to obtain the same operation effect as this embodiment described below.

[First Embodiment]
First, as shown in FIG. 1, a microorganism culturing apparatus A according to the first embodiment includes a culture tank 2 for storing a culture solution (liquid containing a soluble substance) 1 containing halophilic microorganisms together with salt, and in the culture solution. And an air supply system B for supplying air (a gas containing oxygen) G.

  In addition, the air supply system B according to the present embodiment has an air supply pipe disposed by disposing an air diffuser 3 such as an air stone attached to the tip in the culture solution 1 and communicating the inside with the culture solution 1. 4 (4a), an air supply means 5 such as an air supply pump for supplying air G to the inside of the air supply pipe 4 (4b), and an air supply pipe 4 between the air supply means 5 and the air stone 3 And a sterilization filter 6 for sterilizing the air G flowing through the air pipe 4 and an eye for supplying clogging prevention liquid to the inside of the air pipe 4 (4a) (including the air stone 3) The clogging prevention liquid supply means 7 is provided.

  Further, in this air supply system B, the clogging prevention liquid supply means 7 includes a clogging prevention liquid storage tank 9 for storing clogging prevention liquid 8 such as pure water. The air G1 flowing through the inside of the trachea 4 (4b) is supplied into the clogging prevention liquid 8 in the clogging prevention liquid storage tank 9, and the air G2 containing the clogging prevention liquid is used to prevent clogging. The liquid storage tank 9 is configured to be circulated into the air supply tube 4 (4a) and fed into the culture solution 1.

  That is, in the air supply system B of the microorganism culture apparatus A of the present embodiment, water such as pure water (clogging prevention) is provided between the air supply pump (air supply means) 5 and the air stone 3 in the culture tank 2. Bubbling tank (clogging prevention liquid storage tank) 9 for storing air 8 in a sealed state is provided, and air G1 sent from the air supply pump 5 through the sterilization filter 6 is water 8 of the bubbling tank 9. The water 8 is aerated and is aerated. Further, the water 8 in the bubbling tank 9 is aerated, and the air G2 that has floated from the water 8 to the gas phase above the bubbling tank 9 is sent from the bubbling tank 9 to the air stone 3 through the air supply pipe 4 (4a) and cultured. It is configured to be supplied into the culture solution 1 in the tank 2.

  In the microorganism culture apparatus A and the air supply system B of the present embodiment configured as described above, the air G sent from the air supply pump 5 to the air stone 3 passes through the water 8 in the bubbling tank 9 in the middle thereof. Increases the humidity (including clogging prevention liquid). Then, the humidified air G2 flows through the air supply pipe 4 and is sent to the air stone 3, and is supplied to and discharged from the culture solution 1, so that the air supply pipe 4 of the air supply system B is compared with the conventional case. (4a) and the amount of salt deposited / attached in the air stone 3 are greatly reduced.

[Second Embodiment]
Next, as shown in FIG. 2, the microorganism culturing apparatus C according to the second embodiment includes a culture tank 2 for storing a culture solution (liquid containing a soluble substance) 1 containing halophilic microorganisms together with salt, and a culture solution. And an air supply system D for supplying air (a gas containing oxygen) G therein.

  In addition, the air supply system D of the present embodiment is provided with an air supply pipe arranged by disposing an air diffuser 3 such as an air stone attached to the tip in the culture solution 1 and communicating the inside with the culture solution 1. 4, an air supply means 5 such as an air supply pump for supplying and distributing air G inside the air supply pipe 4, and an air supply pipe 4 between the air supply means 5 and the air stone 3. A sterilization filter 6 for sterilizing the air G flowing through 4 and a clogging prevention liquid supply for supplying a clogging prevention liquid to the inside of the air supply pipe 4 (4a) (including the air stone 3) And means 10.

  Further, in this air supply system D, the clogging prevention liquid supply means 10 is provided with a liquid supply means 11 for supplying a clogging prevention liquid 8 such as pure water into the inside of the air supply pipe 4 for clogging prevention. Along with the liquid 8, the air G is circulated inside the air supply pipe 4 and fed into the culture solution 1.

  The liquid supply means 11 of the present embodiment includes a clogging prevention liquid storage tank 9 that stores the clogging prevention liquid 8, and a water supply pipe 12 that is connected to the air supply pipe 4 from the clogging prevention liquid storage tank 9. A liquid supply means 13 such as a water supply pump provided in the middle of the water supply pipe 12 for circulating the clogging prevention liquid 8 from the clogging prevention liquid storage tank 9 to the water supply pipe 12 and supplying it to the air supply pipe 4; And a filter 14 for sterilizing and cleaning the clogging prevention liquid 8 flowing through the water pipe 12.

  That is, in the air supply system D of the microorganism culture apparatus C of the present embodiment, the air G1 sent from the air supply pump (air supply means) 5 through the sterilization filter 6 is directly supplied to the air stone 3. It is comprised so that. On the other hand, in the air supply system D of the microorganism culture apparatus C, liquid supply means 11 for supplying water (clogging prevention liquid) 8 such as pure water into the air supply pipe 4 between the sterilization filter 6 and the air stone 3. Is provided. The liquid supply means 11 includes a water tank (clogging prevention liquid storage tank) 9 for storing water 8 such as pure water, and a water supply pump for supplying the water 8 in the water tank 9 into the air supply pipe 4 through the filter 14. (Liquid feeding means) 13. Further, the water pump 13 is a metering pump capable of supplying a predetermined amount of water as appropriate.

  In the microorganism culture apparatus C and the air supply system D of the present embodiment configured as described above, the air G1 is supplied to the air supply pipe 4 through which the air G1 flows while the air G1 is being supplied from the air supply pump 5 to the air stone 3. An appropriate amount of water 8 is supplied from the liquid means 11. As a result, the water 8 together with the air G flows through the inside of the air pipe 4 and is supplied / released from the air stone 3 into the culture solution 1. In this way, the air G2 containing the water 8 flows through the air supply pipe 4 and is sent to the air stone 3, so that the air G2 in the air supply system D and the air stone 3 in the air supply system D is compared with the conventional case. The amount of salt deposited / attached is greatly reduced.

  Here, the demonstration experiment which confirmed the superiority of the microorganism culture apparatus A and the air supply system B of the first embodiment and the microorganism culture apparatus C and the air supply system D of the second embodiment will be described.

  In this demonstration experiment, first, the aeration rate was 0.2 L / min, the temperature of the culture solution 1 was 40 ° C., and the salt concentration of the culture solution 1 was 20%. Further, a Kinoshita type glass ball filter (air stone) was used as the air diffusion means 3 of the air supply systems B and D.

  Then, a ventilation test was performed for each of the conventional case where direct ventilation was performed, the invention case 1 shown in the first embodiment where pure water bubbling was performed, and the invention case 2 shown in the second embodiment where pure water was added. went. Further, in Invention Case 2, Invention Case 2-1 in which the amount of pure water 8 added is 0.1 mL / min and Invention Case 2-2 in which the amount of pure water 8 added is 0.05 mL / min. A ventilation test was conducted.

  FIG. 3 shows the change over time in the air flow rate in each of the above conditions and in each case. From the results shown in FIG. 3, first, in the conventional case where direct ventilation was performed, the ventilation rate began to decrease immediately after ventilation, and after 24 hours, suddenly decreased to about half of the initially set ventilation rate (0.2 L / min). After 48 hours, clogging occurred due to the salt deposited / adhered to the air stone 3, and almost no aeration was observed. In addition, when the air supply pipe 4 was observed when almost no ventilation was observed (after about 55 hours), it was confirmed that a large amount of salt was deposited inside the pipe and clogging occurred. Moreover, the tube surface was also changed to white, and the adhesion to the tube surface by salt and the accompanying deterioration were confirmed.

  On the other hand, in Invention Case 1 (microorganism culturing apparatus A, air supply system B of the first embodiment) in which pure water bubbling is performed, a slight decrease in aeration (a decrease of about 25%) is first confirmed. It was confirmed that a stable air flow rate can be maintained by ventilating air G2 bubbled with pure water 8 and humidified.

  Next, Invention Case 2 in which a small amount of pure water 8 is added to the air G, Furthermore, Invention Case 2-1 in which the amount of pure water added is 0.1 mL / min, and the amount of pure water added is 0.05 mL / min. It was confirmed that in both cases of the invention case 2-2 that was set to min (microorganism culturing apparatus C, air supply system D of the second embodiment), the amount of aeration during the test period hardly decreased.

  FIG. 4 shows the result of stopping the addition of pure water 8 in the middle of Invention Case 2-2. From this result, it was confirmed that the amount of air flow decreased immediately after the stop, and about 3 hours. Later, it was confirmed that the air flow decreased to about 15% of the set air flow rate.

  Here, when aeration is performed on a liquid such as the culture solution 1, a gas G such as air is sent in a state where the liquid 1 to be aerated is pressurized. For this reason, normally, when the gas G is continuously supplied, the liquid 1 does not flow backward into the air supply pipe 4, and salt (soluble substance) is deposited or adhered in the air supply pipe 4. I don't think it will happen. However, as confirmed in the above-described demonstration experiment, in the conventional case where the ventilation is merely performed, salt is precipitated in the air supply pipe 4 even though the ventilation is continuously performed. Yes.

  On the other hand, the inventor of the present application thought that water vapor containing salt and the like gradually entered the inside of the air supply pipe 4 even in a pressurized state. Then, after ingenuity, as in Invention Case 1 and Invention Case 2, a humidified gas G2 is sent, or a liquid 8 such as water is added to the gas G1, and the gas G2 is sent together with the liquid component 8. It is possible to maintain the humidity in the air supply pipe 4 at a high level by gassing, and the salt (soluble substance) can be obtained by circulating the gas G2 and the liquid component 8 having high humidity in the air supply pipe 4. It was possible to discharge it so that it was washed away, and succeeded in obtaining a particularly remarkable effect of preventing salt from being precipitated inside the air pipe 4.

  Therefore, in the air supply systems B and D of the present embodiment and the microorganism culture apparatuses A and C including the air supply systems B and D, the liquid supply means 7 and 10 for preventing clogging are provided so that air ( It is possible to supply and release the clogging prevention liquid 8 together with the gas G) into the culture liquid 1. And the water 8 of the liquid for clogging prevention as well as the air G distribute | circulates the inside of the air supply pipe 4 conventionally, and the salt content of the soluble substance contained in the culture solution 1 is the air supply system B, It is possible to prevent deposition / adhesion in the inside of the air supply pipe 4 of D.

  Further, the air G flowing through the inside of the air supply pipe 4 by the air supply means 5 such as an air supply pump is supplied into the water (clogging prevention liquid) 8 in the clogging prevention liquid storage tank 9, Moisture (liquid component for preventing clogging) can be included in the air G. That is, the humidity of air can be raised. As a result, moisture can be circulated in the air supply tube 4 together with the air G to be supplied to and discharged from the culture solution 1, and the salt contained in the culture solution 1 is contained in the air supply tube 4 of the air supply system B. Can be prevented from depositing / adhering to the surface.

  Further, by supplying water (clogging prevention liquid) 8 from the liquid supply means 11 to the inside of the air supply pipe 4, the water (clogging prevention liquid part) 8 is circulated along with the air G inside the air supply pipe 4. Thus, it can be supplied to and released from the culture solution 1. Thereby, it can prevent that the salt content contained in the culture solution 1 precipitates / attaches to the inside of the air supply pipe | tube 4 of the air supply system D, etc. FIG.

  At this time, the culture is reliably performed by supplying water (liquid for clogging prevention) 8 so as to be 0.025% by volume or more with respect to the air amount (aeration amount) flowing through the inside of the air supply pipe 4. It is possible to prevent the salt contained in the liquid 1 from being deposited / attached to the inside of the air supply pipe 4 of the air supply system D. That is, when a very small amount of water 8 is added and supplied together with the air G, a remarkable airflow maintaining effect can be obtained with a very small amount of water 8 added. At this time, when water 8 is added at 0.025% of the aeration rate, a remarkable aeration rate maintaining effect can be obtained and the influence on the salinity concentration of the culture solution 1 (reduction in salinity concentration, dilution) ) Is less. By the way, in 5 L culture solution (medium), the amount of water 8 added to the aeration rate of 0.2 L / min is 0.05 mL / min, and this amount is about 72 mL / day and is very small. .

  As a result, by applying the air supply systems B and D to the culture of halophilic microorganisms that need to supply a gas containing oxygen such as air G to the culture solution 1 having a high salinity, In addition, it is possible to effectively prevent salt from depositing / adhering to the inside of the air supply pipes 4 of the air supply systems B and D, and it is possible to perform culturing suitably even for halophilic microorganisms.

  That is, the air supply capacity (air flow capacity) does not decrease at an early stage as in the prior art, and every time the air supply capacity decreases, the air supply pipes 4 of the air supply systems B and D from the culture tank 2, the air stone 3, etc. It is possible to eliminate the need to take out and replace the battery. Alternatively, the frequency of taking out and replacing the air supply systems B and D can be greatly reduced. And since exchange of air supply system B and D becomes unnecessary in this way, it becomes possible to eliminate the blur of culture conditions accompanying this, and it becomes possible to perform stable culture continuously. In addition, it is possible to reduce replacement part costs and culture product loss due to instability of culture conditions.

  Furthermore, since salt content is deposited, clogging does not occur and the aeration volume does not gradually decrease, and the quantitativeness of the aeration volume during culture is maintained. It becomes possible to do. In addition, since the quantitativeness is maintained, the accuracy of the calculated values can be improved and a highly reliable system can be constructed even when engineering design such as ventilation efficiency and biological reaction calculation is performed. .

  Whether the humidification method by bubbling (apparatus A, air supply system B of the first embodiment) or the pure water addition process (apparatus C, air supply system D of the second embodiment) is used. The concentration may be selected according to the concentration of a soluble substance such as the salt content of the liquid 1 to be aerated. For example, when it is applied to supply air in a seawater appreciation water tank or the like, the salinity concentration is relatively low at about 3%, so that a sufficient effect can be obtained even by using a humidified ventilation method. In addition, when applying to air supply under high salinity conditions for research and industrial purposes, it is also effective to apply a method using pure water addition.

  As mentioned above, although one embodiment of the air supply system according to the present invention and the microorganism culturing apparatus provided with the same has been described, the present invention is not limited to the one embodiment described above, and may be appropriately selected without departing from the spirit thereof. It can be changed.

  For example, in the present embodiment, the sterilization filter 6 is provided, but it is not necessary to be limited to the sterilization filter 6 in particular. In particular, when the air G is supplied by the air supply systems B and D to a viewing water tank or the like that does not require contamination or sterilization, the sterilization filter 6 is not necessary.

  Moreover, although the microorganism culture apparatus A and C which perform biological culture were demonstrated, the air supply system B and D which concerns on this invention is gas with respect to the liquid stored in the tank, such as a chemical reaction and a water treatment process. Applicable in any case to supply. That is, it can be applied as various gas aeration means for high salt solution. Needless to say, the present invention can be applied when air is supplied not only to a salt content but also to a liquid having a high concentration of a soluble substance.

1 Culture solution (liquid containing soluble substances)
2 Culture tank 3 Air stone (aeration means)
4 Air supply pipe 5 Air supply pump (air supply means)
6 Disinfection filter 7 Clogging prevention liquid supply means 8 Water (water, clogging prevention liquid, clogging prevention liquid)
9 Bubbling tank, water tank (liquid storage tank for clogging prevention)
10 Liquid supply means 11 for preventing clogging 11 Liquid supply means 12 Water supply pipe 13 Water supply pump (liquid supply means)
14 Filter A Microorganism culture device B Air supply system C Microorganism culture device D Air supply system G Air (gas)

Claims (6)

An air supply system for supplying a gas into a liquid containing a soluble substance,
An air supply pipe disposed in communication with the liquid inside;
Air supply means for circulating gas inside the air supply pipe;
An air supply system comprising: an anti-clogging liquid supply means for supplying an anti-clogging liquid to the inside of the air supply pipe. The air supply system according to claim 1,
The clogging prevention liquid supply means comprises a clogging prevention liquid storage tank for storing clogging prevention liquid,
The gas flowing through the air supply pipe by the air supply means is supplied into the clogging prevention liquid in the clogging prevention liquid storage tank, and the gas containing the clogging prevention liquid is prevented from clogging. An air supply system configured to circulate from the liquid storage tank to the inside of the air supply pipe and supply the liquid into the liquid. The air supply system according to claim 1,
The clogging prevention liquid supply means includes a liquid supply means for supplying clogging prevention liquid into the air supply pipe,
An air supply system configured to circulate gas inside the air supply pipe together with the clogging prevention liquid and supply the gas into the liquid. The air supply system according to claim 3,
The clogging prevention liquid supply means supplies the clogging prevention liquid to the inside of the air supply pipe so that the clogging prevention liquid is contained in an amount of 0.025% by volume or more with respect to the gas. And air supply system. A microorganism culture apparatus for culturing microorganisms in a culture solution stored in a culture tank,
A microorganism culture apparatus comprising the air supply system according to any one of claims 1 to 4 as an air supply system for supplying a gas containing oxygen into the culture solution. The microorganism culture apparatus according to claim 5,
A microorganism culturing apparatus, which is an apparatus for culturing halophilic microorganisms using the culture solution containing salt.

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