JP2014148549A - ヒトM2eペプチド免疫原 - Google Patents
ヒトM2eペプチド免疫原 Download PDFInfo
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- JP2014148549A JP2014148549A JP2014106974A JP2014106974A JP2014148549A JP 2014148549 A JP2014148549 A JP 2014148549A JP 2014106974 A JP2014106974 A JP 2014106974A JP 2014106974 A JP2014106974 A JP 2014106974A JP 2014148549 A JP2014148549 A JP 2014148549A
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Abstract
【解決手段】本発明は、インフルエンザA型ウイルスのM2e標的ペプチドに対する抗体を誘導することができる合成ペプチド免疫原に関する。特に、本発明のペプチド免疫原は1つ以上のエピトープを含む。場合により、ペプチド免疫原は一般的免疫刺激因子をさらに含む。本発明のこれらのペプチド免疫原は、インフルエンザA型ウイルスによる感染を予防するために有効であり、インフルエンザA型ウイルスに対する抗体を誘導することができる。
【選択図】なし
Description
この出願は、2008年11月12日に出願された、仮出願USSN第61/113,880号(この内容は、その全体が参考として本明細書に援用される)に関する。
本発明はまた、以下の項目を提供する。
(項目1)
[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q−[Xaa8−[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q]n
(式中、m、pおよびqは、独立して0または1であり、
nは0〜4の間の任意の数であり、
Xaa0、Xaa6、Xaa7およびXaa8は、独立して任意のアミノ酸であり、
Xaa1−Xaa2−Xaa3−Xaa4−Xaa5は、S−L−L−T−Eであるか、または上記配列S−L−L−T−Eに1個の置換を有するペプチドであって、上記置換は、
Xaa1がCまたはTであり、
Xaa2がA、C、FまたはKであり、
Xaa3がA、C、E、F、I、K、M、Q、S、TまたはVであり、および
Xaa5がDまたはCである
から成る群より選択される)
の配列を含むペプチド免疫原。
(項目2)
Xaa0がCである、項目1に記載のペプチド免疫原。
(項目3)
Xaa6がVまたはCである、項目1に記載のペプチド免疫原。
(項目4)
Xaa7がEである、項目1に記載のペプチド免疫原。
(項目5)
Xaa8がGまたはAである、項目1に記載のペプチド免疫原。
(項目6)
[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q−[Xaa8−[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q]n
(式中、m、pおよびqは、独立して0または1であり、
nは0〜4の間の任意の数であり、
Xaa0、Xaa6、Xaa7およびXaa8は、独立して任意のアミノ酸であり、
Xaa1−Xaa2−Xaa3−Xaa4−Xaa5は、S−L−L−T−Eであるか、または上記配列S−L−L−T−Eに1個の置換を有するペプチドであって、上記置換は、
Xaa1がA、C、D、L、TまたはVであり、
Xaa2がA、C、F、H、I、K、M、N、Q、R、T、WまたはYであり、
Xaa3が任意のアミノ酸であり、
Xaa4がM、N、Q、SまたはWであり、および
Xaa5がA、D、F、H、I、K、M、N、Q、S、W、YまたはCである
から成る群より選択される)
の配列を含むペプチド免疫原。
(項目7)
Xaa0がCである、項目6に記載のペプチド免疫原。
(項目8)
Xaa6がVまたはCである、項目6に記載のペプチド免疫原。
(項目9)
Xaa7がEである、項目6に記載のペプチド免疫原。
(項目10)
Xaa8がGまたはAである、項目6に記載のペプチド免疫原。
(項目11)
上記ペプチドが非線状である、項目1または6に記載のペプチド免疫原。
(項目12)
上記ペプチドが環状(cylic)である、項目11に記載のペプチド免疫原。
(項目13)
上記ペプチドが環状(cylic)であり、およびnが1〜4の間の任意の数である、項目11に記載のペプチド免疫原。
(項目14)
上記ペプチドが、HuMe2抗体8I10または23K12に特異的に結合する、項目1または6に記載のペプチド免疫原。
(項目15)
上記ペプチドがD−アミノ酸を含む、項目1または6に記載のペプチド免疫原。
(項目16)
上記ペプチドが、配列SLLTEVGSLLTEV(配列番号:320)を有する線状または環状ペプチドである、項目1または6に記載のペプチド免疫原。
(項目17)
上記ペプチドが、配列CSLLTEVGSLLTEV(配列番号:283)を有する線状または環状ペプチドである、項目1または6に記載のペプチド免疫原。
(項目18)
上記ペプチドが、配列CSLLTECGSLLTCV(配列番号:463)を有する線状または環状ペプチドである、項目1または6に記載のペプチド免疫原。
(項目19)
上記ペプチドが抗体Z3G1に結合しない、項目1または6に記載のペプチド免疫原。
(項目20)
上記ペプチドがキャリアに結合している、項目1または6に記載のペプチド免疫原。
(項目21)
上記キャリアが、ヒト血清アルブミン、キーホールリンペットヘモシアニン(KLH)、免疫グロブリン分子、サイログロブリン、オボアルブミン、インフルエンザ赤血球凝集素、PAN−DR結合ペプチド(PADREポリペプチド)、マラリアサーカムスポロゾイド(CS)タンパク質、B型肝炎表面抗原(HBSAg19〜28、熱ショックタンパク質(HSP)65、カルメット‐ゲラン杆菌(BCG)、コレラ毒素、毒性を低下させたコレラ毒素変異体、ジフテリア毒素、ジフテリア毒素と交差反応性であるCRM197タンパク質、組換え連鎖球菌性C5aペプチダーゼ、Streptococcus pyogenes ORF1224、Streptococcus pyogenes ORF1664、Streptococcus pyogenes ORF2452、Chlamydia pneumoniae ORF T367、Chlamydia pneumoniae ORF T858、破傷風トキソイド、HIV gp120 T1、接着マトリックス分子を認識する微生物表面成分(MSCRAMMS)、増殖因子/成長ホルモン、サイトカインまたはケモカインである、項目20に記載のペプチド免疫原。
(項目22)
項目1または6に記載のペプチド免疫原を含むワクチン組成物。
(項目23)
無機塩類、GM−CSF、529SE、IL−12、リン酸アルミニウム、水酸化アルミニウム、結核菌、百日咳菌、細菌リポ多糖類、アミノアルキルグルコサンホスフェート化合物、MPL(商標)(3−O−脱アシル化モノホスホリル脂質A)、ポリペプチド、Quil A、STIMULON(商標)QS−21、百日咳毒素(PT)、大腸菌易熱性毒素(LT)、IL−1α、IL−1β、IL−2、IL−4、IL−5、IL−6、IL−7、IL−8、IL−10、IL−13、IL−14、IL−15、IL−16、IL−17、IL−18、インターフェロン−α、インターフェロン−β、インターフェロン−γ、G−CSF、TNF−αおよびTNF−βならびにフロイント完全アジュバント(FCA)から成る群より選択されるアジュバントをさらに含む、項目22に記載のワクチン組成物。
(項目24)
1つ以上の薬学的に許容され得る賦形剤、希釈剤および/またはアジュバントと共に、項目1または6に記載のペプチド免疫原を含む、免疫原性組成物。
(項目25)
上記薬学的に許容され得るアジュバントおよび/またはキャリアが、ミョウバン、リポシン、サポニン、スクアレン、L121、エマルシゲンモノホスホリル脂質A(MPL)、ポリソルベート80、QS21、Montanide ISA51、ISA35、ISA206およびISA720から成る群より選択される、項目24に記載の免疫原性組成物。
(項目26)
項目1または6に記載のペプチド免疫原を哺乳動物に投与することによってインフルエンザウイルス媒介性疾患を予防するまたは治療する方法。
(項目27)
項目22のいずれかに記載のワクチン組成物を哺乳動物に投与することによってインフルエンザウイルス媒介性疾患を予防するまたは治療する方法。
(項目28)
項目24のいずれかに記載の免疫原性組成物を哺乳動物に投与することによってインフルエンザウイルス媒介性疾患を予防するまたは治療する方法。
(項目29)
項目1または6に記載のペプチド免疫原を含むキット。
ペプチド免疫原をスクリーニングするために使用される抗体を、本明細書ではhuM2e抗体と称する。使用されるモノクローナル抗体は、M2エクトドメイン(M2e)に特異的であり、細胞系統において発現される完全長M2に由来する。huM2e抗体は、M2トランスフェクト細胞上の高次構造決定基、ならびにインフルエンザ感染細胞またはウイルス自体の天然M2に結合する。huM2e抗体は線状M2eペプチドに結合しないが、細胞系統へのcDNAトランスフェクション後に発現されるいくつかの自然M2変異体には結合する。ヒトモノクローナル抗体は、非常に広い範囲のインフルエンザA型ウイルス株に対して特異性を示す。
以下の定義は本発明を理解するうえで有用である:
本明細書で使用される「抗体」(Ab)という用語は、モノクローナル抗体、ポリクローナル抗体、多重特異性抗体(たとえば二重特異性抗体)、および所望の生物活性を示す限り、抗体フラグメントを含む。「免疫グロブリン」(Ig)という用語は、本明細書では「抗体」と交換可能に使用される。
本発明は、HuM2e抗体に結合するペプチド免疫原に関する。1つの実施形態では、抗体は、本明細書で8i10、21B15または23K12と称する抗体である。これらの抗体は、同じ細胞型の非感染対照細胞と比較してインフルエンザA型感染細胞への優先的または特異的結合を示すことが公知である。
特に、HuM2e 23k12/8i10に結合する本発明のペプチド免疫原は、S−L−L−T−Eのコア配列ならびにその変異体、改変型および多量体を含む。結合データから導かれる低ストリンジェンシー(0.01μg/mL)条件(図6A)および高ストリンジェンシー(0.001μg/mL)条件(図6B)についてのコア配列およびその変異体を図6Aおよび6Bに示す。
[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q−[Xaa8−[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q]n
(式中、m、pおよびqは、独立して0または1であり、
nは0〜4の間の任意の数であり、
Xaa0は任意のアミノ酸、好ましくはCであり、
Xaa6は任意のアミノ酸、好ましくはVまたはCであり、
Xaa7は任意のアミノ酸、好ましくはEであり、
Xaa8はプロリンを含まない任意のアミノ酸、好ましくはGまたはAであり、
Xaa1−Xaa2−Xaa3−Xaa4−Xaa5は、S−L−L−T−Eであるか、
または配列S−L−L−T−Eに1個の置換を有するペプチドであって、その置換は、
Xaa1がCまたはTであり、
Xaa2がA、C、FまたはKであり、
Xaa3がA、C、E、F、I、K、M、Q、S、TまたはVであり、そして
Xaa5がDまたはCである
から成る群より選択される)
によって表される。
[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q−[Xaa8−[Xaa0]m−Xaa1−Xaa2−Xaa3−Xaa4−Xaa5−[Xaa6]p−[Xaa7]q]n
(式中、m、pおよびqは、独立して0または1であり、
nは0〜4の間の任意の数であり、
Xaa0は任意のアミノ酸、好ましくはCであり、
Xaa6は任意のアミノ酸、好ましくはVまたはCであり、
Xaa7は任意のアミノ酸、好ましくはEであり、
Xaa8はプロリンを含まない任意のアミノ酸、好ましくはGまたはAであり、
Xaa1−Xaa2−Xaa3−Xaa4−Xaa5は、S−L−L−T−Eであるか、または
配列S−L−L−T−Eに1個の置換を有するペプチドであって、その置換は、
Xaa1がA、C、D、L、TまたはVであり、
Xaa2がA、C、F、H、I、K、M、N、Q、R、T、WまたはYであり、
Xaa3が任意のアミノ酸であり、
Xaa4がM、N、Q、SまたはWであり、そして
Xaa5がA、D、F、H、I、K、M、N、Q、S、W、YまたはCである
から成る群より選択される)
によって表される。
線状ペプチド免疫原は、合成によって調製し、次に様々な生物学的アッセイにおいて特定の特徴に関してスクリーニングすることができる。たとえば、Scott,J.K.とG.P.Smith,Science 249:386,1990;Devlin,J.J.ら、Science 24:404,1990;Furka,A.ら、Int.J.Pept.Protein Res.37:487,1991;Lam,K.S.ら、Nature 354:82,1991。
小さな免疫原性分子を大きな「キャリア」分子に結合することによって、その小さな免疫原性分子の免疫原性を高めるアプローチは、数十年間にわたって成功裏に使用されてきた(たとえばGoebelら(1939)J.Exp.Med.69:53参照)。たとえば、多くの免疫原性組成物が記述されており、精製莢膜ポリマー(capsular polymer)をキャリアタンパク質に結合し、この「キャリア効果」を利用することによってより有効な免疫原性組成物が開発されてきた(Schneersonら(1984)Infect.Immun.45:582−591)。
ペプチド免疫原は、インフルエンザ感染を予防するために抗インフルエンザM2媒介性免疫応答を生じさせるワクチンとして使用できる。合成ペプチドは、インビボで有効な免疫応答を誘導するために安定化とアジュバント添加(adjuvantation)の両方を必要とする。化学的および物理的経路を含む様々なプロセスによって媒介される、インビトロおよびインビボでの分解に対して合成ペプチド免疫原を保護するために様々な方法が用いられてきた。(Manning M Cら、Pharmaceutical Research,1989,6:903−918)。
調査中の免疫原に特異的であることが公知の定義されたエピトープ配列に対応する、抗体結合配列をキットにおいて使用することは特に有用である。このキットは、現在使用されているキットよりも特異的な回答を導き、それ故個々の患者のための免疫原ワクチン療法のより良い選択をもたらす。
M2特異的抗体の同定
ヒト血清において同定されたMAbの3つを発現する単核細胞またはB細胞をクローン集団に希釈し、抗体を産生するように誘導した。抗体を含有する上清を、インフルエンザ株であるインフルエンザH3N2亜型からの完全長M2Eタンパク質で安定にトランスフェクトした293FT細胞への結合に関してスクリーニングした。陽性染色/結合を示した上清を、インフルエンザ株、インフルエンザH3N2亜型からの完全長M2Eタンパク質で安定にトランスフェクトした293FT細胞に関しておよび対照としてベクター単独でトランスフェクトした細胞に関して再びスクリーニングした。
ヒト抗インフルエンザモノクローナル抗体のウイルス結合
UV不活化インフルエンザA型ウイルス(A/PR/8/34)(Applied Biotechnologies)を、PBS中1.2μg/ml、25μl/ウエルで384ウエルMaxiSorpプレート(Nunc)にまき、4℃で一晩インキュベートした。次に、プレートをPBSで3回洗浄し、50μl/ウエルのPBS中1%脱脂粉乳でブロックして、その後室温で1時間インキュベートした。PBSによる2回目の洗浄後、3組のMAbを指示された濃度で添加し、プレートを室温で1時間インキュベートした。PBSでさらに洗浄した後、各々のウエルに、25μlの、PBS/1%乳中の1/5000希釈のホースラディッシュペルオキシダーゼ(HRP)結合ヤギ抗ヒトIgG Fc(Pierce)を添加し、プレートを室温で1時間放置した。最終的なPBS洗浄後、HRP基質である1−Step(商標)Ultra−TMB−ELISA(Pierce)を25μl/ウエルで添加し、暗所にて室温で反応を進行させた。25μl/ウエルの1N H2SO4でアッセイを停止させ、450nmの吸光度(A450)をSpectroMax Plusプレートリーダーで読み取った。データを10μg/mlでのMAb 8I10結合の吸光度に正規化する。結果を図2Aおよび2Bに示す。
完全長M2変異体へのヒト抗インフルエンザモノクローナル抗体の結合
M2変異体(インビボで高病原性表現型を有するものを含む)を分析のために選択した。配列については図3A参照。
エピトープブロッキング
MAb 8I10および23K12が同じ部位に結合するかどうかを決定するために、インフルエンザ株A/HK/483/1997配列を示すM2タンパク質をCHO(チャイニーズハムスター卵巣)細胞株DG44において安定に発現させた。細胞解離バッファー(Invitrogen)で細胞を処理し、採取した。1%FBS、0.2%NaN3を含有するPBS(FACSバッファー)中で細胞を洗浄し、100μg/mlのウサギIgGを添加したFACSバッファー中に107細胞/mlで再懸濁した。細胞を、10μg/mlのMAb(または2N9対照)によって4℃で1時間あらかじめ結合させ、次にFACSバッファーで洗浄した。次に、直接結合したAF647−8I10またはAF647−23K12(AlexaFluor(登録商標)647タンパク質標識キット(Invitrogen)で標識した)を使用して、あらかじめブロックした3つの細胞試料を、試料当たり106細胞について1μg/mlで染色した。フローサイトメトリー分析を、FACSCantoを用いて前記のように進行させた。データは、標準誤差を伴う3つの別々の実験からの平均読み出しである。結果を図4に示す。
M2変異体およびトランケート型M2ペプチドへのヒト抗インフルエンザモノクローナル抗体の結合
他のM2ペプチド変異体に対するmAb 8i10および23K12の交差反応性をELISAによって評価した。ペプチド配列を表7Aおよび7Bに示す。加えて、同様のELISAアッセイを用いてM2トランケート型ペプチドに対する結合活性を測定した。
本明細書において引用したすべての公表文献および特許出願は、各々の公表文献または特許出願が具体的且つ個別に参照により組み込まれると指示されているかのごとくに、参照により本明細書に組み込まれる。
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EP2346529B1 (en) | 2016-02-10 |
US20100135955A1 (en) | 2010-06-03 |
TW201029663A (en) | 2010-08-16 |
CA2743306A1 (en) | 2010-05-20 |
JP2012508753A (ja) | 2012-04-12 |
WO2010056796A1 (en) | 2010-05-20 |
JP5693460B2 (ja) | 2015-04-01 |
US20120258077A1 (en) | 2012-10-11 |
US20150125423A1 (en) | 2015-05-07 |
AU2009314091A1 (en) | 2010-05-20 |
EP2346529A1 (en) | 2011-07-27 |
US8932597B2 (en) | 2015-01-13 |
AU2009314091B2 (en) | 2015-05-14 |
US8329188B2 (en) | 2012-12-11 |
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