JP2014128210A5 - - Google Patents
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- JP2014128210A5 JP2014128210A5 JP2012286978A JP2012286978A JP2014128210A5 JP 2014128210 A5 JP2014128210 A5 JP 2014128210A5 JP 2012286978 A JP2012286978 A JP 2012286978A JP 2012286978 A JP2012286978 A JP 2012286978A JP 2014128210 A5 JP2014128210 A5 JP 2014128210A5
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- JP
- Japan
- Prior art keywords
- ips cells
- cells
- hepatocytes
- group
- human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 210000004027 cell Anatomy 0.000 claims 25
- 238000000034 method Methods 0.000 claims 8
- 210000003494 hepatocyte Anatomy 0.000 claims 7
- 210000002966 serum Anatomy 0.000 claims 7
- 238000012258 culturing Methods 0.000 claims 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 4
- 230000002440 hepatic effect Effects 0.000 claims 3
- 210000000130 stem cell Anatomy 0.000 claims 3
- 102000009024 Epidermal Growth Factor Human genes 0.000 claims 2
- 101800003838 Epidermal growth factor Proteins 0.000 claims 2
- 102100035961 Hematopoietically-expressed homeobox protein HHEX Human genes 0.000 claims 2
- 101001021503 Homo sapiens Hematopoietically-expressed homeobox protein HHEX Proteins 0.000 claims 2
- 101001062347 Homo sapiens Hepatocyte nuclear factor 3-beta Proteins 0.000 claims 2
- 101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 claims 2
- 102000004877 Insulin Human genes 0.000 claims 2
- 108090001061 Insulin Proteins 0.000 claims 2
- 102000004140 Oncostatin M Human genes 0.000 claims 2
- 108090000630 Oncostatin M Proteins 0.000 claims 2
- 102100021380 Transcription factor GATA-4 Human genes 0.000 claims 2
- 102000004338 Transferrin Human genes 0.000 claims 2
- 108090000901 Transferrin Proteins 0.000 claims 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 claims 2
- 229960003957 dexamethasone Drugs 0.000 claims 2
- 230000004069 differentiation Effects 0.000 claims 2
- 229940116977 epidermal growth factor Drugs 0.000 claims 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 2
- 229940125396 insulin Drugs 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 229930002330 retinoic acid Natural products 0.000 claims 2
- 239000012581 transferrin Substances 0.000 claims 2
- 229960001727 tretinoin Drugs 0.000 claims 2
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 claims 2
- 239000007952 growth promoter Substances 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012286978A JP6057418B2 (ja) | 2012-12-28 | 2012-12-28 | 人工多能性幹細胞から分化した肝細胞を含む細胞群から、肝細胞からなる細胞培養物を得る方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012286978A JP6057418B2 (ja) | 2012-12-28 | 2012-12-28 | 人工多能性幹細胞から分化した肝細胞を含む細胞群から、肝細胞からなる細胞培養物を得る方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014128210A JP2014128210A (ja) | 2014-07-10 |
| JP2014128210A5 true JP2014128210A5 (enrdf_load_stackoverflow) | 2016-02-18 |
| JP6057418B2 JP6057418B2 (ja) | 2017-01-11 |
Family
ID=51407288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012286978A Expired - Fee Related JP6057418B2 (ja) | 2012-12-28 | 2012-12-28 | 人工多能性幹細胞から分化した肝細胞を含む細胞群から、肝細胞からなる細胞培養物を得る方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6057418B2 (enrdf_load_stackoverflow) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10006005B2 (en) | 2014-06-27 | 2018-06-26 | National University Corporation Chiba University | Culture medium and method for inducing differentiation of pluripotent stem cells to hepatoblasts |
| WO2020075822A1 (ja) | 2018-10-10 | 2020-04-16 | 国立大学法人鳥取大学 | 外来染色体を含むヒト人工多能性幹細胞の製造方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011133599A2 (en) * | 2010-04-20 | 2011-10-27 | International Stem Cell Corporation | Physiological methods for isolation of high purity cell populations |
| JP5845533B2 (ja) * | 2010-10-12 | 2016-01-20 | 株式会社オンチップ・バイオテクノロジーズ | 特定の細胞内に選択的に取り込まれる蛍光色素を利用して多種類の細胞から特定の細胞を純化濃縮する方法 |
| US20190194607A1 (en) * | 2011-01-31 | 2019-06-27 | Dnavec Corporation | Highly functional liver cells derived from pluripotent stem cells, method for producing same, and method for testing metabolism/toxicity of drug |
-
2012
- 2012-12-28 JP JP2012286978A patent/JP6057418B2/ja not_active Expired - Fee Related
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