JP2013531992A5 - - Google Patents

Claims (15)

(A) providing a fermentation broth comprising a recombinant microorganism that produces product alcohol from a fermentable carbon source, wherein the recombinant microorganism comprises a reduction or elimination of pyruvate decarboxylase activity;
(B) contacting the fermentation broth with a fermentable carbon source, whereby the recombinant microorganism consumes the fermentable carbon source and produces the product alcohol;
(C) contacting the fermentation broth with a fatty acid derived from biomass in a step in the fermentation process, wherein (i) growth rate and (ii) fermentability of the recombinant microorganism in the presence of the fatty acid A process wherein at least one of the carbon consumption is higher than the growth rate and / or the fermentable carbon consumption of the recombinant microorganism in the absence of the fatty acid.   The method of claim 1, wherein steps (b) and (c) are performed substantially simultaneously.   The method of claim 1, wherein the fermentable carbon source is derived from the biomass. A method for producing a product alcohol comprising:
(A) providing a biomass comprising a fermentable carbon source and oil;
(B) converting at least a portion of the oil into fatty acids to form biomass containing the fatty acids;
(C) contacting the biomass with a fermentation broth containing a recombinant microorganism capable of producing a product alcohol from a fermentable carbon source, wherein the recombinant microorganism reduces or eliminates pyruvate decarboxylase activity. Including a process;
(D) contacting the fatty acid with the fermentation broth, wherein at least one of (i) growth rate and (ii) fermentable carbon consumption of the recombinant microorganism in the presence of the fatty acid is free of the fatty acid. Higher than the growth rate of the recombinant microorganism and / or the fermentable carbon consumption; optionally further fermenting the fermentable carbon source to produce a product alcohol.   The step (b) of converting at least a part of the oil into a fatty acid comprises contacting the oil with one or more substances capable of hydrolyzing a part of the oil into a fatty acid. Preferably, the one or more substances comprise one or more enzymes, more preferably the one or more enzymes comprise a lipase enzyme, optionally prior to step (c), 5. The method of claim 4, further comprising inactivating the one or more enzymes after at least a portion of the oil has been hydrolyzed.   One or more of steps (b), (c), and (d) are performed in the fermentor, or one of steps (b), (c), and (d) 6. A method according to claim 4 or 5, wherein or more are performed substantially simultaneously. Before the step (b) of converting at least a part of the oil into fatty acids, further comprising the step of separating the oil from the biomass, or by liquefying the biomass to produce liquefied biomass And wherein the liquefied biomass comprises an oligosaccharide;
Further comprising the step of contacting the liquefied biomass with a saccharification enzyme capable of converting oligosaccharides into fermentable sugars to form saccharified biomass,
The method according to any one of claims 4 to 6, wherein step (c) comprises contacting the saccharified biomass with the fermentation broth containing recombinant microorganisms. A method for producing a product alcohol comprising:
(A) providing a raw material;
(B) liquefying the raw material to produce a raw material slurry;
(C) separating the raw slurry and producing a product comprising (i) an aqueous layer containing a fermentable carbon source, (ii) an oil layer, and (iii) a solid layer;
(D) obtaining oil from the oil layer and converting at least a portion of the oil into fatty acids;
(E) supplying the aqueous layer of (c) to a fermentor containing a fermentation broth containing a recombinant microorganism capable of producing a product alcohol from a fermentable carbon source, wherein the recombinant microorganism comprises: Comprising reducing or eliminating pyruvate decarboxylase activity;
(F) fermenting the fermentable carbon source of the aqueous layer to produce the product alcohol;
(G) contacting the fermentation broth with the fatty acid, wherein at least one of (i) growth rate and (ii) fermentable carbon consumption of the recombinant microorganism in the presence of the fatty acid is free of the fatty acid The growth rate of said recombinant microorganism and / or a step higher than said fermentable carbon consumption.   The step (d) of converting at least a part of the oil into a fatty acid comprises contacting the oil with one or more substances capable of hydrolyzing a part of the oil into a fatty acid. 9. The method of claim 8, wherein preferably the one or more substances comprise one or more enzymes, more preferably the one or more enzymes comprises a lipase enzyme.   The method according to any one of claims 1 to 9, wherein the fatty acid is selected from oleic acid, palmitic acid, myristic acid, and mixtures thereof. Biomass is crop residue such as corn kernel, corn cobs, corn peel, corn stover, grass, wheat, rye, wheat straw, barley, barley straw, pasture, rice straw, switchgrass, waste paper Sugarcane pomace, sorghum, sugarcane, soy, ingredients obtained from grinding cereals, cellulosic materials, lignocellulosic materials, trees, branches, roots, leaves, wood chips, sawdust, shrubs and shrubs, vegetables, fruits, 8. A method according to any one of claims 1 to 7 derived from flowers, animal manure, and mixtures thereof.   12. A process according to any one of the preceding claims, wherein the product alcohol is butanol.   The method according to claim 1, wherein the fermentation broth further comprises ethanol.   14. A method according to any one of claims 1 to 13, wherein the recombinant microorganism has one or more pyruvate decarboxylase (PDC) gene deletions.   Ingredients are crop residues such as corn kernels, corn cob, corn peel, corn stover, grass, wheat, rye, wheat straw, barley, barley straw, pasture, rice straw, switchgrass, waste paper , Sugarcane pomace, sorghum, sugarcane, soybeans, ingredients obtained from grinding cereals, cellulosic materials, lignocellulosic materials, trees, branches, roots, leaves, wood chips, sawdust, shrubs and shrubs, vegetables, fruits, 10. The method of claim 8 or 9, comprising one or more fermentable sugars derived from flowers, animal manure, and mixtures thereof.