AR083153A1 - PRODUCTION OF ESTERES OF ALCOHOLS AND IN-SITU SEPARATION OF THE PRODUCT DURING THE FERMENTATION OF ALCOHOL, FERMENTATION SOIL AND YEAST CELL - Google Patents

PRODUCTION OF ESTERES OF ALCOHOLS AND IN-SITU SEPARATION OF THE PRODUCT DURING THE FERMENTATION OF ALCOHOL, FERMENTATION SOIL AND YEAST CELL

Info

Publication number
AR083153A1
AR083153A1 ARP110102142A ARP110102142A AR083153A1 AR 083153 A1 AR083153 A1 AR 083153A1 AR P110102142 A ARP110102142 A AR P110102142A AR P110102142 A ARP110102142 A AR P110102142A AR 083153 A1 AR083153 A1 AR 083153A1
Authority
AR
Argentina
Prior art keywords
alcohol
fermentation
carboxylic acid
alcohol esters
product
Prior art date
Application number
ARP110102142A
Other languages
Spanish (es)
Inventor
Keith H Burlew
Michael Dauner
Michael Charles Grady
Douglas Robert Anton
Robert Dicosimo
Brian Michael Roesch
Original Assignee
Butamax Tm Advanced Biofuels
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/160,766 external-priority patent/US9012190B2/en
Application filed by Butamax Tm Advanced Biofuels filed Critical Butamax Tm Advanced Biofuels
Publication of AR083153A1 publication Critical patent/AR083153A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C29/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom not belonging to a six-membered aromatic ring
    • C07C29/74—Separation; Purification; Use of additives, e.g. for stabilisation
    • C07C29/76—Separation; Purification; Use of additives, e.g. for stabilisation by physical treatment
    • C07C29/86—Separation; Purification; Use of additives, e.g. for stabilisation by physical treatment by liquid-liquid treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Liquid Carbonaceous Fuels (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Fodder In General (AREA)
  • Fats And Perfumes (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Proceso de fermentación alcohólica y composición que incluye la producción de ésteres de alcohol por esterificación del producto de alcohol en un medio de fermentación con un ácido carboxílico (por ejemplo, ácido graso) y un catalizador (por ejemplo, lipasa) capaz de esterificar el producto de alcohol, tal como butanol, con el ácido carboxílico para formar los ésteres de alcohol. Los ésteres de alcohol pueden extraerse del medio de fermentación, y el producto de alcohol recuperarse de los ésteres de alcohol. El ácido carboxílico puede servir, además, como un extractante para separar los ésteres de alcohol del medio de fermentación.Reivindicación 1: Un método para producir ésteres de butilo que comprende poner en contacto butanol producido en un proceso de fermentación con al menos un ácido carboxílico y al menos un catalizador capaz de esterificar el ácido carboxílico con el butanol para formar ésteres de butilo del ácido carboxílico; en donde el ácido carboxílico en el proceso de fermentación está presente en una concentración suficiente para producir una mezcla de dos fases. Reivindicación 4: El método de acuerdo con la reivindicación 1, en donde la materia prima en el proceso de fermentación comprende uno o más azúcares fermentables derivados del grano de maíz, mazorca de maíz, residuos de cultivos tales como cascarillas, rastrojos de maíz, hierbas, trigo, centeno, paja de trigo, cebada, paja de cebada, heno, paja de arroz, pasto varilla, residuos de papel, bagazo de caña de azúcar, sorgo, caña de azúcar, soya, componentes obtenidos de la molienda de granos, material celulósico, material lignocelulósico, árboles, ramas, raíces, hojas, astillas de madera, serrín, arbustos y malezas, vegetales, frutas, flores, estiércol animal, y mezclas de éstos. Reivindicación 164: Un caldo de fermentación que comprende: (a) un microorganismo recombinante capaz de producir un producto de alcohol; (b) un sustrato de carbono fermentable; (c) un catalizador capaz de esterificar los ácidos grasos extracelularmente con dicho producto de alcohol en ésteres de alcohol de ácido graso; y (d) ésteres de alcohol de ácido graso, en donde los ésteres de alcohol de ácido graso se producen durante la fermentación. Reivindicación 174: Una célula de levadura recombinante capaz de producir isobutanol, la célula de levadura comprende una ruta biosintética del isobutanol en el cual dicha ruta biosintética del isobutanol comprende al menos un enzima que cataliza cada una de las siguientes conversiones de sustrato a producto: i) piruvato en acetolactato; ii) acetolactato en 2,3-dihidroxiisovalerato; iii) 2,3-dihidroxiisovalerato en a-cetoisovalerato; iv) a-cetoisovalerato en isobutiraldehído; y v) isobutiraldehído en isobutanol; y en donde al menos una de la enzima de iii) o la enzima de v) se codifican por los polinucleótidos heterólogos integrados en el cromosoma.Alcoholic fermentation process and composition that includes the production of alcohol esters by esterification of the alcohol product in a fermentation medium with a carboxylic acid (for example, fatty acid) and a catalyst (for example, lipase) capable of esterifying the product of alcohol, such as butanol, with the carboxylic acid to form the alcohol esters. The alcohol esters can be extracted from the fermentation medium, and the alcohol product can be recovered from the alcohol esters. The carboxylic acid can also serve as an extractant to separate the alcohol esters from the fermentation medium. Claim 1: A method of producing butyl esters comprising contacting butanol produced in a fermentation process with at least one carboxylic acid and at least one catalyst capable of esterifying the carboxylic acid with butanol to form carboxylic acid butyl esters; wherein the carboxylic acid in the fermentation process is present in a concentration sufficient to produce a two-phase mixture. Claim 4: The method according to claim 1, wherein the raw material in the fermentation process comprises one or more fermentable sugars derived from corn grain, corn cob, crop residues such as husks, corn stubble, herbs , wheat, rye, wheat straw, barley, barley straw, hay, rice straw, rod grass, paper waste, sugarcane bagasse, sorghum, sugarcane, soy, components obtained from grain milling, cellulosic material, lignocellulosic material, trees, branches, roots, leaves, wood chips, sawdust, shrubs and weeds, vegetables, fruits, flowers, animal manure, and mixtures thereof. Claim 164: A fermentation broth comprising: (a) a recombinant microorganism capable of producing an alcohol product; (b) a fermentable carbon substrate; (c) a catalyst capable of esterifying fatty acids extracellularly with said alcohol product in fatty acid alcohol esters; and (d) fatty acid alcohol esters, wherein fatty acid alcohol esters are produced during fermentation. Claim 174: A recombinant yeast cell capable of producing isobutanol, the yeast cell comprises a biosynthetic route of isobutanol in which said biosynthetic route of isobutanol comprises at least one enzyme that catalyzes each of the following substrate to product conversions: ) pyruvate in acetolactate; ii) acetolactate in 2,3-dihydroxyisovalerate; iii) 2,3-dihydroxyisovalerate in a-ketoisovalerate; iv) a-ketoisovalerate in isobutyraldehyde; and v) isobutyraldehyde in isobutanol; and wherein at least one of the iii) enzyme or the v) enzyme is encoded by the heterologous polynucleotides integrated into the chromosome.

ARP110102142A 2010-06-18 2011-06-21 PRODUCTION OF ESTERES OF ALCOHOLS AND IN-SITU SEPARATION OF THE PRODUCT DURING THE FERMENTATION OF ALCOHOL, FERMENTATION SOIL AND YEAST CELL AR083153A1 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US35629010P 2010-06-18 2010-06-18
US36844410P 2010-07-28 2010-07-28
US36845110P 2010-07-28 2010-07-28
US36842910P 2010-07-28 2010-07-28
US36843610P 2010-07-28 2010-07-28
US37954610P 2010-09-02 2010-09-02
US201161440034P 2011-02-07 2011-02-07
US13/160,766 US9012190B2 (en) 2011-06-15 2011-06-15 Use of thiamine and nicotine adenine dinucleotide for butanol production

Publications (1)

Publication Number Publication Date
AR083153A1 true AR083153A1 (en) 2013-02-06

Family

ID=45348555

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110102142A AR083153A1 (en) 2010-06-18 2011-06-21 PRODUCTION OF ESTERES OF ALCOHOLS AND IN-SITU SEPARATION OF THE PRODUCT DURING THE FERMENTATION OF ALCOHOL, FERMENTATION SOIL AND YEAST CELL

Country Status (13)

Country Link
EP (4) EP2582827A2 (en)
JP (4) JP2013533742A (en)
KR (4) KR20130045330A (en)
CN (4) CN102947457B (en)
AR (1) AR083153A1 (en)
AU (4) AU2011268201A1 (en)
BR (3) BR112012032166A2 (en)
CA (4) CA2801498A1 (en)
MX (4) MX2012014683A (en)
NZ (3) NZ603467A (en)
RU (1) RU2013102308A (en)
WO (4) WO2011159998A2 (en)
ZA (3) ZA201208372B (en)

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