JP2013523181A5 - - Google Patents
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- Publication number
- JP2013523181A5 JP2013523181A5 JP2013505078A JP2013505078A JP2013523181A5 JP 2013523181 A5 JP2013523181 A5 JP 2013523181A5 JP 2013505078 A JP2013505078 A JP 2013505078A JP 2013505078 A JP2013505078 A JP 2013505078A JP 2013523181 A5 JP2013523181 A5 JP 2013523181A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- cell
- endogenous protein
- human
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 26
- 102000004169 proteins and genes Human genes 0.000 claims 13
- 108090000623 proteins and genes Proteins 0.000 claims 13
- 238000000034 method Methods 0.000 claims 11
- 108010042407 Endonucleases Proteins 0.000 claims 10
- 102000004533 Endonucleases Human genes 0.000 claims 10
- 230000002759 chromosomal effect Effects 0.000 claims 7
- 238000003776 cleavage reaction Methods 0.000 claims 6
- 230000007017 scission Effects 0.000 claims 6
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 4
- 102000040430 polynucleotide Human genes 0.000 claims 4
- 108091033319 polynucleotide Proteins 0.000 claims 4
- 239000002157 polynucleotide Substances 0.000 claims 4
- 238000011144 upstream manufacturing Methods 0.000 claims 4
- NPOAOTPXWNWTSH-UHFFFAOYSA-N 3-hydroxy-3-methylglutaric acid Chemical compound OC(=O)CC(O)(C)CC(O)=O NPOAOTPXWNWTSH-UHFFFAOYSA-N 0.000 claims 2
- 102000007469 Actins Human genes 0.000 claims 2
- 108010085238 Actins Proteins 0.000 claims 2
- 102000009012 HMGA Proteins Human genes 0.000 claims 2
- 108010049069 HMGA Proteins Proteins 0.000 claims 2
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 claims 2
- 108010047294 Lamins Proteins 0.000 claims 2
- 102000006835 Lamins Human genes 0.000 claims 2
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 claims 2
- 102000004243 Tubulin Human genes 0.000 claims 2
- 108090000704 Tubulin Proteins 0.000 claims 2
- 230000005782 double-strand break Effects 0.000 claims 2
- 210000005053 lamin Anatomy 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
Applications Claiming Priority (15)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32369810P | 2010-04-13 | 2010-04-13 | |
| US32371910P | 2010-04-13 | 2010-04-13 | |
| US32370210P | 2010-04-13 | 2010-04-13 | |
| US61/323,702 | 2010-04-13 | ||
| US61/323,698 | 2010-04-13 | ||
| US61/323,719 | 2010-04-13 | ||
| US36701710P | 2010-07-23 | 2010-07-23 | |
| US61/367,017 | 2010-07-23 | ||
| US39066810P | 2010-10-07 | 2010-10-07 | |
| US61/390,668 | 2010-10-07 | ||
| US40885610P | 2010-11-01 | 2010-11-01 | |
| US61/408,856 | 2010-11-01 | ||
| US201161431957P | 2011-01-12 | 2011-01-12 | |
| US61/431,957 | 2011-01-12 | ||
| PCT/US2011/032218 WO2011130346A1 (en) | 2010-04-13 | 2011-04-13 | Methods for generating endogenously tagged protein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013523181A JP2013523181A (ja) | 2013-06-17 |
| JP2013523181A5 true JP2013523181A5 (cg-RX-API-DMAC7.html) | 2014-05-08 |
| JP5841997B2 JP5841997B2 (ja) | 2016-01-13 |
Family
ID=44799004
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013505077A Active JP5841996B2 (ja) | 2010-04-13 | 2011-04-13 | 異種タンパク質を発現するための内因性プロモーターの使用 |
| JP2013505078A Active JP5841997B2 (ja) | 2010-04-13 | 2011-04-13 | 内因性にタグ付けされたタンパク質を生成するための方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013505077A Active JP5841996B2 (ja) | 2010-04-13 | 2011-04-13 | 異種タンパク質を発現するための内因性プロモーターの使用 |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US20130059388A1 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2558574B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP5841996B2 (cg-RX-API-DMAC7.html) |
| KR (2) | KR20130055588A (cg-RX-API-DMAC7.html) |
| CN (3) | CN107805278A (cg-RX-API-DMAC7.html) |
| BR (1) | BR112012026379A2 (cg-RX-API-DMAC7.html) |
| CA (2) | CA2795636C (cg-RX-API-DMAC7.html) |
| DK (1) | DK2558575T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2565216T3 (cg-RX-API-DMAC7.html) |
| WO (3) | WO2011130346A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3489359A1 (en) | 2010-07-23 | 2019-05-29 | Sigma Aldrich Co. LLC | Genome editing using targeting endonucleases and single-stranded nucleic acids |
| US9012617B2 (en) * | 2011-04-11 | 2015-04-21 | The Trustees Of The University Of Pennsylvania | Detection reagents for tyrosine kinase activity and methods of use thereof |
| KR20250035015A (ko) * | 2011-12-05 | 2025-03-11 | 팩터 바이오사이언스 인크. | 세포를 형질감염시키는 방법들 및 생성물들 |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US10274499B2 (en) | 2012-03-27 | 2019-04-30 | The University Of Western Ontario | SH2 domain variants |
| WO2013158309A2 (en) * | 2012-04-18 | 2013-10-24 | The Board Of Trustees Of The Leland Stanford Junior University | Non-disruptive gene targeting |
| EP4556564A3 (en) * | 2012-10-23 | 2025-09-03 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| AU2014235794A1 (en) | 2013-03-14 | 2015-10-22 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| CA2939847C (en) | 2014-03-21 | 2023-09-05 | The Board Of Trustees Of The Leland Stanford Junior University | Genome editing without nucleases |
| RS58627B2 (sr) * | 2014-04-01 | 2022-06-30 | Biontech Cell&Gene Therapies Gmbh | Klaudin-6-specifični imuno receptori i t-ćelijski epitopi |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| EP3268394B1 (en) * | 2015-03-13 | 2020-05-27 | University of Maryland, Baltimore | Universal antibody-mediated biosensor |
| US11667911B2 (en) | 2015-09-24 | 2023-06-06 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/CAS-mediated genome editing |
| PT3371314T (pt) * | 2015-11-04 | 2023-08-31 | Fate Therapeutics Inc | Modificação genómica de células pluripotentes |
| EP3394245A1 (en) * | 2015-12-23 | 2018-10-31 | Teknologian Tutkimuskeskus VTT OY | A method for obtaining indicator signals from a cell |
| WO2017165826A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP4047092B1 (en) | 2016-04-13 | 2025-07-30 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| CN106800602B (zh) * | 2017-01-22 | 2020-05-01 | 中国科学院生物物理研究所 | 一种可视化标记基因组位点的方法 |
| WO2018148603A1 (en) * | 2017-02-09 | 2018-08-16 | Allen Institute | Genetically-tagged stem cell lines and methods of use |
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| WO2019118879A1 (en) * | 2017-12-14 | 2019-06-20 | Donald Danforth Plant Science Center | Homologous recombination via transcriptional activation |
| KR20200141473A (ko) * | 2018-05-04 | 2020-12-18 | 시그마-알드리치 컴퍼니., 엘엘씨 | 숙주 세포 단백질의 감소된 수준을 갖는 재조합 단백질 생산 |
| WO2020141109A1 (en) * | 2018-12-30 | 2020-07-09 | F. Hoffmann-La Roche Ag | Method for the selection of cells based on crispr/cas-controlled integration of a detectable tag to a target protein |
| EP4031583A4 (en) * | 2019-09-16 | 2023-11-15 | Fred Hutchinson Cancer Center | CHIMERIC RECEPTOR PROTEINS AND THEIR USES |
| CN113684228B (zh) * | 2021-10-26 | 2022-03-11 | 天九再生医学(天津)科技有限公司 | 利用内参快速准确表征蛋白质相对丰度的方法 |
| CN114703174B (zh) * | 2022-04-12 | 2023-10-24 | 中国科学院海洋研究所 | 快速获得基因型和表型突变的CRISPR/Cas9基因敲除方法及应用 |
| CN115948401A (zh) * | 2022-12-19 | 2023-04-11 | 中国科学技术大学 | 一种多靶点rna靶向敲低系统及应用 |
| CN120177781B (zh) * | 2025-05-22 | 2025-08-12 | 南京师范大学 | 生物传感器及其应用和高通量筛选花姜酮生产菌的方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7192772B1 (en) * | 1988-08-31 | 2007-03-20 | The University Of Florida Research Foundations, Inc. | Recombinant cells that highly express chromosomally-integrated heterologous gene |
| US7090976B2 (en) * | 1999-11-10 | 2006-08-15 | Rigel Pharmaceuticals, Inc. | Methods and compositions comprising Renilla GFP |
| US6495664B1 (en) * | 1998-07-24 | 2002-12-17 | Aurora Biosciences Corporation | Fluorescent protein sensors of post-translational modifications |
| US6534261B1 (en) * | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
| US6710170B2 (en) * | 1999-09-10 | 2004-03-23 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of ovarian cancer |
| US7125660B2 (en) * | 2000-09-13 | 2006-10-24 | Archemix Corp. | Nucleic acid sensor molecules and methods of using same |
| WO2003027253A2 (en) * | 2001-09-26 | 2003-04-03 | University Of North Carolina At Chapel Hill | Variants of alpha-fetoprotein coding and expression sequences |
| CA2534296C (en) * | 2003-08-08 | 2013-03-26 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| US7402436B2 (en) * | 2004-05-03 | 2008-07-22 | City Of Hope | Lentiviral vectors for site-specific gene insertion |
| JP2008507995A (ja) * | 2004-08-02 | 2008-03-21 | セルーメン、インコーポレイテッド | 細胞内での分子相互作用の検出方法 |
| CA2579677A1 (en) * | 2004-09-16 | 2006-03-30 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
| US20060171929A1 (en) * | 2005-01-31 | 2006-08-03 | The University Of Washington | Regulation of dendritic cell functions by the DCAL-2 receptor |
| WO2006094073A2 (en) * | 2005-03-02 | 2006-09-08 | Acadia Pharmaceuticals Inc. | Functional bioluminescence energy resonance transfer (bret) assay to screen, identify and characterize receptor tyrosine kinase ligands |
| EP1869206A4 (en) * | 2005-03-02 | 2011-08-10 | Einstein Coll Med | ENZYME SENSORS WITH ENVIRONMENTAL SENSITIVE OR FLUORESCENT LABELS AND APPLICATIONS THEREOF |
| US8114964B2 (en) * | 2005-05-19 | 2012-02-14 | Centocor, Inc. | Anti-MCP-1 antibodies, compositions, methods and uses |
| US20070155014A1 (en) * | 2005-07-20 | 2007-07-05 | Invitrogen Corporation | Methods for increasing efficiency of homologous recombination |
| US20080305519A1 (en) * | 2006-02-23 | 2008-12-11 | Qing Lin | Biochemical method for specific protein labeling |
| WO2007139895A2 (en) * | 2006-05-24 | 2007-12-06 | Cellumen, Inc. | Method for modeling a disease |
| AU2008317354B2 (en) * | 2007-10-25 | 2014-04-10 | Ospedale San Raffaele S.R.L. | Methods and compositions for targeted integration |
| EP2281050B1 (en) * | 2008-04-14 | 2014-04-02 | Sangamo BioSciences, Inc. | Linear donor constructs for targeted integration |
| CA2734235C (en) * | 2008-08-22 | 2019-03-26 | Sangamo Biosciences, Inc. | Methods and compositions for targeted single-stranded cleavage and targeted integration |
-
2011
- 2011-04-13 WO PCT/US2011/032218 patent/WO2011130346A1/en not_active Ceased
- 2011-04-13 US US13/641,023 patent/US20130059388A1/en not_active Abandoned
- 2011-04-13 KR KR1020127028401A patent/KR20130055588A/ko not_active Ceased
- 2011-04-13 CN CN201711044283.XA patent/CN107805278A/zh active Pending
- 2011-04-13 ES ES11769489.3T patent/ES2565216T3/es active Active
- 2011-04-13 CN CN2011800291603A patent/CN102971421A/zh active Pending
- 2011-04-13 KR KR1020127028946A patent/KR20130054955A/ko not_active Ceased
- 2011-04-13 BR BR112012026379A patent/BR112012026379A2/pt not_active Application Discontinuation
- 2011-04-13 DK DK11769489.3T patent/DK2558575T3/en active
- 2011-04-13 CN CN201180029129.XA patent/CN102959078B/zh active Active
- 2011-04-13 US US13/641,050 patent/US20130059362A1/en not_active Abandoned
- 2011-04-13 CA CA2795636A patent/CA2795636C/en active Active
- 2011-04-13 WO PCT/US2011/032214 patent/WO2011130343A1/en not_active Ceased
- 2011-04-13 WO PCT/US2011/032216 patent/WO2011130345A1/en not_active Ceased
- 2011-04-13 EP EP11769488.5A patent/EP2558574B1/en active Active
- 2011-04-13 US US13/641,036 patent/US20130065310A1/en not_active Abandoned
- 2011-04-13 CA CA2795643A patent/CA2795643C/en active Active
- 2011-04-13 JP JP2013505077A patent/JP5841996B2/ja active Active
- 2011-04-13 JP JP2013505078A patent/JP5841997B2/ja active Active
- 2011-04-13 EP EP11769489.3A patent/EP2558575B1/en active Active
-
2017
- 2017-05-31 US US15/609,983 patent/US20180105564A1/en not_active Abandoned
- 2017-07-19 US US15/654,419 patent/US20180134759A1/en not_active Abandoned
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